Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	3,007,831	G→T	A108S (GCC→TCC)	ygeX →	2,3‑diaminopropionate ammonia‑lyase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	3,007,831	0	G	T	100.0%	217.5 / NA	62	A108S (GCC→TCC)	ygeX	2,3‑diaminopropionate ammonia‑lyase
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base T (33/29);  total (33/29)

TCGTCAAAGACGAATCAAAACGATTCGGTCTGAACGCCTTCAAAATGCTTGGCGGTGCGTACGCCATCGCTCAATTATTGTGTGAAAAATATCATCTTGATATCGAAACGCTGTCATTTGAGCACCTGAAAAATGCCATCGGCGAAAAAATGACTTTCGCGACCACCACCGACGGCAACCACGGGCGCGGTGTGGCGTGGGCAGCACAGCAACTCGGACAGAATGCGGTGATTTACATGCCGAAAGGTTCTGCTCAGGAACGCGTTGACGC  >  NC_000913/3007697‑3007967                                                                                                                                       |                                                                                                                                         tcGTCAAAGACGAATCAAAACGATTCGGTCTGAACGCCTTCAAAATGCTTGGCGGTGCGTACGCCATCGCTCAATTATTGTGTGAAAAATATCATCTTGATATCGAAACGCTGTCATTTGAGCACCTGAAAAATTCCATc                                                                                                                                     >  2:1074283/1‑140 (MQ=255)                   aaCGATTCGGTCTGAACGCCTTCAAAATGCTTGGCGGTGCGTACGCCATCGCTCAATTATTGTGTGAAAAATATCATCTTGATATCGAAACGCTGTCATTTGAGCACCTGAAAAATTCCATCGGCGAAAAAATGACTTTc                                                                                                                   <  2:1157313/140‑1 (MQ=255)                        ttCGGTCTGAACGCCTTCAAAATGCTTGGCGGTGCGTACGCCATCGCTCAATTATTGTGTGAAAAATATCATCTTGATATCGAAACGCTGTCATTTGAGCACCTGAAAAATTCCATCGGCGAAAAAATGACTTTCGCGac                                                                                                              <  1:99528/140‑1 (MQ=255)                                gAACGCCTTCAAAATGCTTGGCGGTGCGTACGCCATCGCTCAATTATTGTGTGAAAAATATCATCTTGATATCGAAACGCTGTCATTTGAGCACCTGAAAAATTCCATCGGCGAAAAAATGACTTTCGCGACCACCACCg                                                                                                      >  2:355835/1‑140 (MQ=255)                                   cGCCTTCAAAATGCTTGGCGGTGCGTACGCCATCGCTCAATTATTGTGTGAAAAATATCATCTTGATATCGAAACGCTGTCATTTGAGCACCTGAAAAATTCCATCGGCGAAAAAATGACTTTCGCGACCACCACCGACg                                                                                                   <  2:899138/140‑1 (MQ=255)                                      cTTCAAAATGCTTGGCGGTGCGTACGCCATCGCTCAATTATTGTGTGAAAAATATCATCTTGATATCGAAACGCTGTCATTTGAGCACCTGAAAAATTCCATCGGCGAAAAAATGACTTTCGCGACCACCACCGACGGCa                                                                                                >  1:362888/1‑140 (MQ=255)                                       ttCAAAATGCTTGGCGGTGCGTACGCCATCGCTCAATTATTGTGTGAAAAATATCATCTTGATATCGAAACGCTGTCATTTGAGCACCTGAAAAATTCCATCGGCGAAAAAATGACTTTCGCGACCACCACCGACGGCaa                                                                                               >  2:610637/1‑140 (MQ=255)                                          aaaaTGCTTGGCGGTGCGTACGCCATCGCTCAATTATTGTGTGAAAAATATCATCTTGATATCGAAACGCTGTCATTTGAGCACCTGAAAAATTCCATCGGCGAAAAAATGACTTTCGCGACCACCACCGACGGCAACCa                                                                                            <  1:1364653/140‑1 (MQ=255)                                               gCTTGGCGGTGCGTACGCCATCGCTCAATTATTGTGTGAAAAATATCATCTTGATATCGAAACGCTGTCATTTGAGCACCTGAAAAATTCCATCGGCGAAAAAATGACTTTCGCGACCACCACCGACGGCAACCACGGgc                                                                                       <  1:388391/140‑1 (MQ=255)                                                cTTGGCGGTGCGTACGCCATCGCTCAATTATTGTGTGAAAAATATCATCTTGATATCGAAACGCTGTCATTTGAGCACCTGAAAAATTCCATCGGCGAAAAAATGACTTTCGCGACCACCACCGACGGCAACCACGGgcg                                                                                      >  1:211570/1‑140 (MQ=255)                                                   ggCGGTGCGTACGCCATCGCTCAATTATTGTGTGAAAAATATCATCTTGATATCGAAACGCTGTCATTTGAGCACCTGAAAAATTCCATCGGCGAAAAAATGACTTTCGCGACCACCACCGACGGCAACCACGGGCGCgg                                                                                   >  2:1411069/1‑140 (MQ=255)                                                   ggCGGTGCGTACGCCATCGCTCAATTATTGTGTGAAAAATATCATCTTGATATCGAAACGCTGTCATTTGAGCACCTGAAAAATTCCATCGGCGAAAAAATGACTTTCGCGACCACCACCGACGGCAACCACGGGCGCgg                                                                                   >  1:1432881/1‑140 (MQ=255)                                                   ggCGGTGCGTACGCCATCGCTCAATTATTGTGTGAAAAATATCATCTTGATATCGAAACGCTGTCATTTGAGCACCTGAAAAATTCCATCGGCGAAAAAATGACTTTCGCGACCACCACCGACGGCAACCACGGGCGCgg                                                                                   >  1:584759/1‑140 (MQ=255)                                                    gCGGTGCGTACGCCATCGCTCAATTATTGTGTGAAAAATATCATCTTGATATCGAAACGCTGTCATTTGAGCACCTGAAAAATTCCATCGGCGAAAAAATGACTTTCGCGACCACCACCGACGGCAACCACGGGCGCGgt                                                                                  <  2:362888/140‑1 (MQ=255)                                                       gTGCGTACGCCATCGCTCAATTATTGTGTGAAAAATATCATCTTGATATCGAAACGCTGTCATTTGAGCACCTGAAAAATTCCATCGGCGAAAAAATGACTTTCGCGACCACCACCGACGGCAACCACGGGCGCGGtgtg                                                                               >  1:14615/1‑140 (MQ=255)                                                            tACGCCATCGCTCAATTATTGTGTGAAAAATATCATCTTGATATCGAAACGCTGTCATTTGAGCACCTGAAAAATTCCATCGGCGAAAAAATGACTTTCGCGACCACCACCGACGGCAACCACGGGCGCGGTGTGGCGTg                                                                          <  1:610637/140‑1 (MQ=255)                                                               gCCATCGCTCAATTATTGTGTGAAAAACATCATCTTGATATCGAAACGCTGTCATTTGAGCACCTGAAAAATTCCATCGGCGAAAAAATGACTTTCGCGACCACCACCGACGGCAACCACGGGCGCGGTGTGGCGTGGgc                                                                       >  2:952371/1‑140 (MQ=255)                                                                  aTCGCTCAATTATTGTGTGAAAAATATCATCTTGATATCGAAACGCTGTCATTTGAGCACCTGAAAAATTCCATCGGCGAAAAAATGACTTTCGCGACCACCACCGACGGCAACCACGGGCGCGGTGTGGCGTGGgcagc                                                                    >  1:598205/1‑140 (MQ=255)                                                                    cGCTCAATTATTGTGTGAAAAATATCATCTTGATATCGAAACGCTGTCATTTGAGCACCTGAAAAATTCCATCGGCGAAAAAATGACTTTCGCGACCACCACCGACGGCAACCACGGGCGCGGTGTGGCGTGGGcagcac                                                                  >  2:232455/1‑140 (MQ=255)                                                                           ttattGTGTGAAAAATATCATCTTGATATCGAAACGCTGTCATTTGAGCACCTGAAAAATTCCATCGGCGAAAAAATGACTTTCGCGACCACCACCGACGGCAACCACGGGCGCGGTGTGGCGTGGGCAGCACAGCAACt                                                           >  2:1207041/1‑140 (MQ=255)                                                                             attGTGTGAAAAATATCATCTTGATATCGAAACGCTGTCATTTGAGCACCTGAAAAATTCCATCGGCGAAAAAATGACTTTCGCGACCACCACCGACGGCAACCACGGGCGCGGTGTGGCGTGGGCAGCACAGCAACTCg                                                         >  1:102480/1‑140 (MQ=255)                                                                                gtgtgAAAAATATCATCTTGATATCGAAACGCTGTCATTTGAGCACCTGAAAAATTCCATCGGCGAAAAAATGACTTTCGCGACCACCACCGACGGCAACCACGGGCGCGGTGTGGCGTGGGCAGCACAGCAACTCGGAc                                                      <  1:355835/140‑1 (MQ=255)                                                                                 tgtgAAAAATATCATCTTGATATCGAAACGCTGTCATTTGAGCACCTGAAAAATTCCATCGGCGAAAAAATGACTTTCGCGACCACCACCGACGGCAACCACGGGCGCGGTGTGGCGTGGGCAGCACAGCAACTCGGACa                                                     >  2:675579/1‑140 (MQ=255)                                                                                     aaaaaTATCATCTTGATATCGAAACGCTGTCATTTGAGCACCTGAAAAATTCCATCGGCGAAAAAATGACTTTCGCGACCACCACCGACGGCAACCACGGGCGCGGTGTGGCGTGGGCGACCCAGCAACTCGGACAGAAt                                                 >  2:496423/1‑140 (MQ=255)                                                                                      aaaaTATCATCTTGATATCGAAACGCTGTCATTTGAGCACCTGAAAAATTCCATCGGCGAAAAAATGACTTTCGCGACCACCACCGACGGCAACCACGGGCGCGGTGTGGCGTGGGCAGCACAGCAACTCGGACAGAATg                                                <  2:1204466/140‑1 (MQ=255)                                                                                        aaTATCATCTTGATATCGAAACGCTGTCATTTGAGCACCTGAAAAATTCCATCGGCGAAAAAATGACTTTCGCGACCACCACCGACGGCAACCACGGGCGCGGTGTGGCGTGGGCAGCACAGCAACTCGGACAGAATGCg                                              <  1:684470/140‑1 (MQ=255)                                                                                          tatCATCTTGATATCGAAACGCTGTCATTTGAGCACCTGAAAAATTCCATCGGCGAAAAAATGACTTTCGCGACCACCACCGACGGCAACCACGGGCGCGGTGTGGCGTGGGCAGCACAGCAACTCGGACAGAATGCGGt                                            <  2:211570/140‑1 (MQ=255)                                                                                           atcatcTTGATATCGAAACGCTGTCATTTGAGCACCTGAAAAATTCCATCGGCGAAAAAATGACTTTCGCGACCACCACCGACGGCAACCACGGGCGCGGTGTGGCGTGGGCAGCACAGCAACTCGGa                                                       <  2:501409/128‑1 (MQ=255)                                                                                           atcatcTTGATATCGAAACGCTGTCATTTGAGCACCTGAAAAATTCCATCGGCGAAAAAATGACTTTCGCGACCACCACCGACGGCAACCACGGGCGCGGTGTGGCGTGGGCAGCACAGCAACTCGGa                                                       >  1:501409/1‑128 (MQ=255)                                                                                             catcTTGATATCGAAACGCTGTCATTTGAGCACCTGAAAAATTCCATCGGCGAAAAAATGACTTTCGCGACCACCACCGACGGCAACCACGGGCGCGGTGTGGCGTGGGCAGCACAGCAACTCGGACAGAATGCGGTGAt                                         >  2:208004/1‑140 (MQ=255)                                                                                               tcTTGATATCGAAACGCTGTCATTTGAGCACCTGAAAAATTCCATCGGCGAAAAAATGACTTTCGCGACCACCACCGACGGCAACCACGGGCGCGGTGTGGCGTGGGCAGCACAGCAACTCGGACAGAATGCGGTGAttt                                       >  2:447649/1‑140 (MQ=255)                                                                                                 ttGATATCGAAACGCTGTCATTTGAGCACCTGAAAAATTCCATCGGCGAAAAAATGACTTTCGCGACCACCACCGACGGCAACCACGGGCGCGGTGTGGCGTGGGCAGCACAGCAACTCGGACAGAATGCGGTGATTTAc                                     <  2:584759/140‑1 (MQ=255)                                                                                                    atatCGAAACGCTGTCATTTGAGCACCTGAAAAATTCCATCGGCGAAAAAATGACTTTCGCGACCACCACCGACGGCAACCACGGGCGCGGTGTGGCGTGGGCAGCACAGCAACTCGGACAGAATGCGGTGATTTACATg                                  >  2:987114/1‑140 (MQ=255)                                                                                                    atatCGAAACGCTGTCATTTGAGCACCTGAAAAATTCCATCGGCGAAAAAATGACTTTCGCGACCACCACCGACGGCAACCACGGGCGCGGTGTGGCGTGGGCAGCACAGCAACTCGGACAGAATGCGGTGATTTACATg                                  <  1:447649/140‑1 (MQ=255)                                                                                                         gAAACGCTGTCATTTGAGCACCTGAAAAATTCCATCGGCGAAAAAATGACTTTCGCGACCACCACCGACGGCAACCACGGGCGCGGTGTGGCGTGGGCAGCACAGCAACTCGGACAGAATGCGGTGATTTACATGCCGaa                             <  1:1159450/140‑1 (MQ=255)                                                                                                          aaaCGCTGTCATTTGAGCACCTGAAAAATTCCATCGGCGAAAAAATGACTTTCGCGACCACCACCGACGGCAACCACGGGCGCGGTGTGGCGTGGGCAGCACAGCAACTCGGACAGAATGCGGTGATTTACATGCCGaaa                            >  2:672045/1‑140 (MQ=255)                                                                                                          aaaCGCTGTCATTTGAGCACCTGAAAAATTCCATCGGCGAAAAAATGACTTTCGCGACCACCACCGACGGCAACCACGGGCGCGGTGTGGCGTGGGCAGCACAGCAACTCGGACAGAATGCGGTGATTTACATGCCGaaa                            >  1:43787/1‑140 (MQ=255)                                                                                                          aaaCGCTGTCATTTGAGCACCTGAAAAATTCCATCGGCGAAAAAATGACTTTCGCGACCACCACCGACGGCAACCACGGGCGCGGTGTGGCGTGGGCAGCACAGCAACTCGGACAGAATGCGGTGATTTACATGCCGaaa                            >  2:519249/1‑140 (MQ=255)                                                                                                          aaaCGCTGTCATTTGAGCACCTGAAAAATTCCATCGGCGAAAAAATGACTTTCGCGACCACCACCGACGGCAACCACGGGCGCGGTGTGGCGTGGGCAGCACAGCAACTCGGACAGAATGCGGTGATTTACATGCCGaaa                            >  2:529860/1‑140 (MQ=255)                                                                                                           aaCGCTGTCATTTGAGCACCTGAAAAATTCCATCGGCGAAAAAATGACTTTCGCGACCACCACCGACGGCAACCACGGGCGCGGTGTGGCGTGGGCAGCACAGCAACTCGGACAGAATGCGGTGATTTACATGCCGAAAg                           >  1:136074/1‑140 (MQ=255)                                                                                                            aCGCTGTCATTTGAGCACCTGAAAAATTCCATCGGCGAAAAAATGACTTTCGCGACCACCACCGACGGCAACCACGGGCGCGGTGTGGCGTGGGCAGCACAGCAACTCGGACAGAATGCGGTGATTTACATGCCGAAAgg                          <  2:136074/140‑1 (MQ=255)                                                                                                              gCTGTCATTTGAGCACCTGAAAAATTCCATCGGCGAAAAAATGACTTTCGCGACCACCACCGACGGCAACCACGGGCGCGGTGTGGCGTGGGCAGCACAGCAACTCGGACAGAATGCGGTGATTTACATGCCGAAAGGtt                        <  2:82949/140‑1 (MQ=255)                                                                                                              gCTGTCATTTGAGCACCTGAAAAATTCCATCGGCGAAAAAATGACTTTCGCGACCACCACCGACGGCAACCACGGGCGCGGTGTGGCGTGGGCAGCACAGCAACTCGGACAGAATGCGGTGATTTACATGCCGAAAGGtt                        <  2:43787/140‑1 (MQ=255)                                                                                                              gCTGTCATTTGAGCACCTGAAAAATTCCATCGGCGAAAAAATGACTTTCGCGACCACCACCGACGGCAACCACGGGCGCGGTGTGGCGTGGGCAGCACAGCAACTCGGACAGAATGCGGTGATTTACATGCCGAAAGGtt                        <  2:285889/140‑1 (MQ=255)                                                                                                              gCTGTCATTTGAGCACCTGAAAAATTCCATCGGCGAAAAAATGACTTTCGCGACCACCACCGACGGCAACCACGGGCGCGGTGTGGCGTGGGCAGCACAGCAACTCGGACAGAATGCGGTGATTTACATGCCGAAAGGtt                        <  2:1138951/140‑1 (MQ=255)                                                                                                              gCTGTCATTTGAGCACCTGAAAAATTCCATCGGCGAAAAAATGACTTTCGCGACCACCACCGACGGCAACCACGGGCGCGGTGTGGCGTGGGCAGCACAGCAACTCGGACAGAATGCGGTGATTTACATGCCGAAAGGtt                        <  2:1380053/140‑1 (MQ=255)                                                                                                              gCTGTCATTTGAGCACCTGAAAAATTCCATCGGCGAAAAAATGACTTTCGCGACCACCACCGACGGCAACCACGGGCGCGGTGTGGCGTGGGCAGCACAGCAACTCGGACAGAATGCGGTGATTTACATGCCGAAAGGtt                        <  2:1354771/140‑1 (MQ=255)                                                                                                               cTGTCATTTGAGCACCTGAAAAATTCCATCGGCGAAAAAATGACTTTCGCGACCACCACCGACGGCAACCACGGGCGCGGTGTGGCGTGGGCAGCACAGCAACTCGGACAGAATGCGGTGATTTACATGCCGAAAGGttt                       >  1:958941/1‑139 (MQ=255)                                                                                                                 gTCATTTGAGCACCTGAAAAATTCCATCGGCGAAAAAATGACTTTCGCGACCACCACCGACGGCAACCACGGGCGCGGTGTGGCGTGGGCAGCACAGCAACTCGGACAGAATGCGGTGATTTACATGCCGAAAGGTTCTg                     >  1:1249975/1‑140 (MQ=255)                                                                                                                 gTCATTTGAGCACCTGAAAAATTCCATCGGCGAAAAAATGACTTTCGCGACCACCACCGACGGCAACCACGGGCGCGGTGTGGCGTGGGCAGCACAGCAACTCGGACAGAATGCGGTGATTTACATGCCGAAAGGTTCTg                     >  1:762824/1‑140 (MQ=255)                                                                                                                 gTCATTTGAGCACCTGAAAAATTCCATCGGCGAAAAAATGACTTTCGCGACCACCACCGACGGCAACCACGGGCGCGGTGTGGCGTGGGCAGCACAGCAACTCGGACAGAATGCGGTGATTTACATGCCGAAAGGTTCTg                     >  1:528673/1‑140 (MQ=255)                                                                                                                 gTCATTTGAGCACCTGAAAAATTCCATCGGCGAAAAAATGACTTTCGCGACCACCACCGACGGCAACCACGGGCGCGGTGTGGCGTGGGCAGCACAGCAACTCGGACAGAATGCGGTGATTTACATGCCGAAAGGTTCTg                     >  1:390441/1‑140 (MQ=255)                                                                                                                     tttGAGCACCTGAAAAATTCCATCGGCGAAAAAATGACTTTCGCGACCACCACCGACGGCAACCACGGGCGCGGTGTGGCGTGGGCAGCACAGCAACTCGGACAGAATGCGGTGATTTACATGCCGAAAGGTTCTGCTCa                 >  2:363639/1‑140 (MQ=255)                                                                                                                         aGCACCTGAAAAATTCCATCGGCGAAAAAATGACTTTCGCGACCACCACCGACGGCAACCACGGGCGCGGTGTGGCGTGGGCAGCACAGCAACTCGGACAGAATGCGGTGATTTACATGCCGAAAGGTTCTGCTCAGGaa             >  2:1008074/1‑140 (MQ=255)                                                                                                                          gCACCTGAAAAATTCCATCGGCGAAAAAATGACTTTCGCGACCACCACCGACGGCAACCACGGGCGCGGTGTGGCGTGGGCAGCACAGCAACTCGGACAGAATGCGGTGATTTACATGCCGAAAGGTTCTGCTCAGGAAc            <  1:496423/140‑1 (MQ=255)                                                                                                                            aCCTGAAAAATTCCATCGGCGAAAAAATGACTTTCGCGACCACCACCGACGGCAACCACGGGCGCGGTGTGGCGTGGGCAGCACAGCAACTCGGACAGAATGCGGTGATTTACATGCCGAAAGGTTCTGCTCAGGAAcgc          >  1:814368/1‑140 (MQ=255)                                                                                                                                   aaaTTCCATCGGCGAAAAAATGACTTTCGCGACCACCACCGACGGCAACCACGGGCGCGGTGTGGCGTGGGCAGCACAGCAACTCGGACAGAATGCGGTGATTTACATGCCGAAAGGTTCTGCTCAGGAACGCGTTGACg   <  2:762824/140‑1 (MQ=255)                                                                                                                                   aaaTTCCATCGGCGAAAAAATGACTTTCGCGACCACCACCGACGGCAACCACGGGCGCGGTGTGGCGTGGGCAGCACAGCAACTCGGACAGAATGCGGTGATTTACATGCCGAAAGGTTCTGCTCAGGAACGCGTTGACg   <  1:1008074/140‑1 (MQ=255)                                                                                                                                   aaaTTCCATCGGCGAAAAAATGACTTTCGCGACCACCACCGACGGCAACCACGGGCGCGGTGTGGCGTGGGCAGCACAGCAACTCGGACAGAATGCGGTGATTTACATGCCGAAAGGTTCTGCTCAGGAACGCGTTGACg   <  1:529860/140‑1 (MQ=255)                                                                                                                                    aaTTCCATCGGCGAAAAAATGACTTTCGCGACCACCACCGACGGCAACCACGGGCGCGGTGTGGCGTGGGCAGCACAGCAACTCGGACAGAATGCGGTGATTTACATGCCGAAAGGTTCTGCTCAGGAACGCGTTGACGc  <  2:626830/140‑1 (MQ=255)                                                                                                                                    aaTTCCATCGGCGAAAAAATGACTTTCGCGACCACCACCGACGGCAACCACGGGCGCGGTGTGGCGTGGGCAGCACAGCAACTCGGACAGAATGCGGTGATTTACATGCCGAAAGGTTCTGCTCAGGAACGCGTTGACGc  <  1:347852/140‑1 (MQ=255)                                                                                                                                    aaTTCCATCGGCGAAAAAATGACTTTCGCGACCACCACCGACGGCAACCACGGGCGCGGTGTGGCGTGGGCAGCACAGCAACTCGGACAGAATGCGGTGATTTACATGCCGAAAGGTTCTGCTCAGGAACGCGTTGACGc  <  2:14615/140‑1 (MQ=255)                                                                                                                                       |                                                                                                                                         TCGTCAAAGACGAATCAAAACGATTCGGTCTGAACGCCTTCAAAATGCTTGGCGGTGCGTACGCCATCGCTCAATTATTGTGTGAAAAATATCATCTTGATATCGAAACGCTGTCATTTGAGCACCTGAAAAATGCCATCGGCGAAAAAATGACTTTCGCGACCACCACCGACGGCAACCACGGGCGCGGTGTGGCGTGGGCAGCACAGCAACTCGGACAGAATGCGGTGATTTACATGCCGAAAGGTTCTGCTCAGGAACGCGTTGACGC  >  NC_000913/3007697‑3007967

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 23 ≤ ATCG/ATCG < 29 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑