Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	398,317	C→A	A149E (GCG→GAG)	yaiW →	surface‑exposed outer membrane lipoprotein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	398,317	0	C	A	100.0%	254.2 / NA	71	A149E (GCG→GAG)	yaiW	surface‑exposed outer membrane lipoprotein
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base A (35/36);  total (35/36)

GCAAGAGATTGACCGTCGTGCTGAACGGATGCACATTCCTGCTTTTCTGGTTCATACCGCGCTAAAAATCAAATCGCCAAACGGCAAAAGTTATAGCGAACGGTTAGATTCGGTGCGTACAGAAAAGCAATTGAGCGCGATTTTTGACGATCTGATCAACATGGTGCCAATGGGGCAGACGCTGTTTGGTTCGCTCAATCCGGTGCGCACCGGTGGGCCGATGCAGGTCAGCATTGCTTTTGCCGAACAGCATACCAAAGGGTATCCG  >  NC_000913/398180‑398447                                                                                                                                          |                                                                                                                                   gCAAGAGATTGACCGTCGTGCTGAACGGATGCACATTCCTGCTTTTCTGGTTCATACCGCGCTAAAAATCAAATCGCCAAACGGCAAAAGTTATAGCGAACGGTTAGATTCGGTGCGTACAGAAAAGCAATTGAGCgaga                                                                                                                                  >  2:1077145/1‑140 (MQ=255)          tGACCGTCGTGCTGAACGGATGCACATTCCTGCTTTTCTGGTTCATCCCGCGCTAAAAATCAAATCGCAAAACGGCAAAAGTTATAGCGAACGGTTAGATTCGGTGCGTACAGAAAAGCAATTGAGCGAGATTTTTGACg                                                                                                                         <  2:1391348/140‑1 (MQ=255)          tGACCGTCGTGCTGAACGGATGCACATTCCTGCTTTTCTGGTTCATACCGCGCTAAAAATCAAATCGCCAAACGGCAAAAGTTATAGCGAACGGTTAGATTCGGTGCGTACAGAAAAGCAATTGAGCGAGATTTTTGACg                                                                                                                         <  1:759412/140‑1 (MQ=255)           gACCGTCGTGCTGAACGGATGCACATTCCTGCTTTTCTGGTTCATACCGCGCTAAAAATCAAATCGCCAAACGGCAAAAGTTATAGCGAACGGTTAGATTCGGTGCGTACAGAAAAGCAATTGAGCGAGATTTTTGACGa                                                                                                                        <  1:425557/140‑1 (MQ=255)            aCCGTCGTGCTGAACGGATGCACATTCCTGCTTTTCTGGTTCATACCGCGCTAAAAATCAAATCGCCAAACGGCAAAAGTTATAGCGAACGGTTAGATTCGGTGCGTACAGAAAAGCAATTGAGCGAGATTTTTGACGAt                                                                                                                       <  2:1190340/140‑1 (MQ=255)            aCCGTCGTGCTGAACGGATGCACATTCCTGCTTTTCTGGTTCATACCGCGCTAAAAATCAAATCGCCAAACGGCAAAAGTTATAGCGAACGGTTAGATTCGGTGCGTACAGAAAAGCAATTGAGCGAGATTTTTGACGAt                                                                                                                       <  1:415193/140‑1 (MQ=255)                  gtGCTGAACGGATGCACATTCCTGCTTTTCTGGTTCATACCGCGCTAAAAATCAAATCGCCAAACGGCAAAAGTTATAGCGAACGGTTAGATTCGGTGCGTACAGAAAAGCAATTGAGCGAGATTTTTGACGATCTGATc                                                                                                                 <  1:1245871/140‑1 (MQ=255)                   tGCTGAACGGATGCACATTCCTGCTTTTCTGGTTCATACCGCGCTAAAAATCAAATCGCCAAACGGCAAAAGTTATAGCGAACGGTTAGATTCGGTGCGTACAGAAAAGCAATTGAGCGAGATTTTTGACGATCTGATCa                                                                                                                >  2:1248727/1‑140 (MQ=255)                      tGAACGGATGCACATTCCTGCTTTTCTGGTTCATACCGCGCTAAAAATCAAATCGCCAAACGGCAAAAGTTATAGCGAACGGTTAGATTCGGTGCGTACAGAAAAGCAATTGAGCGAGATTTTTGACGATCTGATCAACa                                                                                                             <  2:858155/140‑1 (MQ=255)                      tGAACGGATGCACATTCCTGCTTTTCTGGTTCATACCGCGCTAAAAATCAAATCGCCAAACGGCAAAAGTTATAGCGAACGGTTAGATTCGGTGCGTACAGAAAAGCAATTGAGCGAGATTTTTGACGATCTGATCAACa                                                                                                             <  1:240067/140‑1 (MQ=255)                             aTGCACATTCCTGCTTTTCTGGTTCATACCGCGCTAAAAATCAAATCGCCAAACGGCAAAAGTTATAGCGAACGGTTAGATTCGGTGCGTACAGAAAAGCAATTGAGCGAGATTTTTGACGATCTGATCAACAt                                                                                                            <  2:966751/134‑1 (MQ=255)                             aTGCACATTCCTGCTTTTCTGGTTCATACCGCGCTAAAAATCAAATCGCCAAACGGCAAAAGTTATAGCGAACGGTTAGATTCGGTGCGTACAGAAAAGCAATTGAGCGAGATTTTTGACGATCTGATCAACAt                                                                                                            >  1:966751/1‑134 (MQ=255)                             aTGCACATTCCTGCTTTTCTGGTTCATACCGCGCTAAAAATCAAATCGCCAAACGGCAAAAGTTATAGCGAACGGTTAGATTCGGTGCGTACAGAAAAGCAATTGAGCGAGATTTTTGACGATCTGATCAACATGGt                                                                                                         <  1:1120073/137‑1 (MQ=255)                             aTGCACATTCCTGCTTTTCTGGTTCATACCGCGCTAAAAATCAAATCGCCAAACGGCAAAAGTTATAGCGAACGGTTAGATTCGGTGCGTACAGAAAAGCAATTGAGCGAGATTTTTGACGATCTGATCAACATGGt                                                                                                         >  2:1120073/1‑137 (MQ=255)                                       cTGCTTTTCTGGTTCATACCGCGCTAAAAATCAAATCGCCAAACGGCAAAAGTTATAGCGAACGGTTAGATTCGGTGCGTACAGAAAAGCAATTGAGCGAGATTTTTGACGATCTGATCAACATGGTGCCAATGGGGCAg                                                                                            >  2:252440/1‑140 (MQ=255)                                       cTGCTTTTCTGGTTCATACCGCGCTAAAAATCAAATCGCCAAACGGCAAAAGTTATAGCGAACGGTTAGATTCGGTGCGTACAGAAAAGCAATTGAGCGAGATTTTTGACGATCTGATCAACATGGTGCCAATGGGGCAg                                                                                            >  1:698565/1‑140 (MQ=255)                                       cTGCTTTTCTGGTTCATACCGCGCTAAAAATCAAATCGCCAAACGGCAAAAGTTATAGCGAACGGTTAGATTCGGTGCGTACAGAAAAGCAATTGAGCGAGATTTTTGACGATCTGATCAACATGGTGCCAATGGGGCAg                                                                                            >  2:305220/1‑140 (MQ=255)                                         gCTTTTCTGGTTCATACCGCGCTAAAAATCAAATCGCCAAACGGCAAAAGTTATAGCGAACGGTTAGATTCGGTGCGTACAGAAAAGCAATTGAGCGAGATTTTTGACGATCTGATCAACATGGTGCCAATGGGGCAGAc                                                                                          <  1:1393296/140‑1 (MQ=255)                                         gCTTTTCTGGTTCATACCGCGCTAAAAATCAAATCGCCAAACGGCAAAAGTTATAGCGAACGGTTAGATTCGGTGCGTACAGAAAAGCAATTGAGCGAGATTTTTGACGATCTGATCAACATGGTGCCAATGGGGCAGAc                                                                                          <  2:1044741/140‑1 (MQ=255)                                             ttCTGGTTCATACCGCGCTAAAAATCAAATCGCCAAACGGCAAAAGTTATAGCGAACGGTTAGATTCGGTGCGTACAGAAAAGCAATTGAGCGAGATTTTTGACGATCTGATCAACATGGTGCCAATGGGGCAGACGCTg                                                                                      >  1:22973/1‑140 (MQ=255)                                              tCTGGTTCATACCGCGCTAAAAATCAAATCGCCAAACGGCAAAAGTTATAGCGAACGGTTAGATTCGGTGCGTACAGAAAAGCAATTGAGCGAGATTTTTGACGATCTGATCAACATGGTGCCAATGGGGCAGACGCTGt                                                                                     <  1:723383/140‑1 (MQ=255)                                               cTGGTTCATACCGCGCTAAAAATCAAATCGCCAAACGGCAAAAGTTATAGCGAACGGTTAGATTCGGTGCGTACAGAAAAGCAATTGAGCGAGATTTTTGACGATCTGATCAACATGGTGCCAATGGGGCAGACg                                                                                         >  2:1375534/1‑135 (MQ=255)                                               cTGGTTCATACCGCGCTAAAAATCAAATCGCCAAACGGCAAAAGTTATAGCGAACGGTTAGATTCGGTGCGTACAGAAAAGCAATTGAGCGAGATTTTTGACGATCTGATCAACATGGTGCCAATGGGGCAGACg                                                                                         <  1:1375534/135‑1 (MQ=255)                                               cTGGTTCATACCGCGCTAAAAATCAAATCGCCAAACGGCAAAAGTTATAGCGAACGGTTAGATTCGGTGCGTACAGAAAAGCAATTGAGCGAGATTTTTGACGATCTGATCAACATGGTGCCAATGGGGCAGACGCTGtt                                                                                    >  2:523312/1‑140 (MQ=255)                                               cTGGTTCATACCGCGCTAAAAATCAAATCGCCAAACGGCAAAAGTTATAGCGAACGGTTAGATTCGGTGCGTACAGAAAAGCAATTGAGCGAGATTTTTGACGATCTGATCAACATGGTGCCAATGGGGCAGACGCTGtt                                                                                    >  1:907927/1‑140 (MQ=255)                                                 ggTTCATACCGCGCTAAAAATCAAATCGCCAAACGGCAAAAGTTATAGCGAACGGTTAGATTCGGTGCGTACAGAAAAGCAATTGAGCGAGATTTTTGACGATCTGATCAACATGGTGCCAATGGGGCAGACGCTGTTTg                                                                                  <  1:1172754/140‑1 (MQ=255)                                                            cgcTAAAAATCAAATCGCCAAACGGCAAAAGTTATAGCGAACGGTTAGATTCGGTGCGTACAGAAAAGCAATTGAGCGAGATTTTTGACGATCTGATCAACATGGTGCCAATGGGGCAGACGCTGTTTGGTTCGCTCAAt                                                                       >  1:1434736/1‑140 (MQ=255)                                                            cgcTAAAAATCAAATCGCCAAACGGCAAAAGTTATAGCGAACGGTTAGATTCGGTGCGTACAGAAAAGCAATTGAGCGAGATTTTTGACGATCTGATCAACATGGTGCCAATGGGGCAGACGCTGTTTGGTTCGCTCAAt                                                                       >  1:1133889/1‑140 (MQ=255)                                                            cgcTAAAAATCAAATCGCCAAACGGCAAAAGTTATAGCGAACGGTTAGATTCGGTGCGTACAGAAAAGCAATTGAGCGAGATTTTTGACGATCTGATCAACATGGTGCCAATGGGGCAGACGCTGTTTGGTTCGCTCAAt                                                                       >  1:1315853/1‑140 (MQ=255)                                                            cgcTAAAAATCAAATCGCCAAACGGCAAAAGTTATAGCGAACGGTTAGATTCGGTGCGTACAGAAAAGCAATTGAGCGAGATTTTTGACGATCTGATCAACATGGTGCCAATGGGGCAGACGCTGTTTGGTTCGCTCAAt                                                                       >  1:967892/1‑140 (MQ=255)                                                            cgcTAAAAATCAAATCGCCAAACGGCAAAAGTTATAGCGAACGGTTAGATTCGGTGCGTACAGAAAAGCAATTGAGCGAGATTTTTGACGATCTGATCAACATGGTGCCAATGGGGCAGACGCTGTTTGGTTCGCTCAAt                                                                       >  1:955351/1‑140 (MQ=255)                                                            cgcTAAAAATCAAATCGCCAAACGGCAAAAGTTATAGCGAACGGTTAGATTCGGTGCGTACAGAAAAGCAATTGAGCGAGATTTTTGACGATCTGATCAACATGGTGCCAATGGGGCAGACGCTGTTTGGTTCGCTCAAt                                                                       >  2:833905/1‑140 (MQ=255)                                                            cgcTAAAAATCAAATCGCCAAACGGCAAAAGTTATAGCGAACGGTTAGATTCGGTGCGTACAGAAAAGCAATTGAGCGAGATTTTTGACGATCTGATCAACATGGTGCCAATGGGGCAGACGCTGTTTGGTTCGCTCAAt                                                                       >  1:656174/1‑140 (MQ=255)                                                            cgcTAAAAATCAAATCGCCAAACGGCAAAAGTTATAGCGAACGGTTAGATTCGGTGCGTACAGAAAAGCAATTGAGCGAGATTTTTGACGATCTGATCAACATGGTGCCAATGGGGCAGACGCTGTTTGGTTCGCTCAAt                                                                       >  2:491871/1‑140 (MQ=255)                                                            cgcTAAAAATCAAATCGCCAAACGGCAAAAGTTATAGCGAACGGTTAGATTCGGTGCGTACAGAAAAGCAATTGAGCGAGATTTTTGACGATCTGATCAACATGGTGCCAATGGGGCAGACGCTGTTTGGTTCGCTCAAt                                                                       >  1:1243547/1‑140 (MQ=255)                                                            cgcTAAAAATCAAATCGCCAAACGGCAAAAGTTATAGCGAACGGTTAGATTCGGTGCGTACAGAAAAGCAATTGAGCGAGATTTTTGACGATCTGATCAACATGGTGCCAATGGGGCAGACGCTGTTTGGTTCGCTCAAt                                                                       >  1:361234/1‑140 (MQ=255)                                                              cTAAAAATCAAATCGCCAAACGGCAAAAGTTATAGCGAACGGTTAGATTCGGTGCGTACAGAAAAGCAATTGAGCGAGATTTTTGACGATCTGATCAACATGGTGCCAATGGGGCAGACGCTGTTTGGTTCGCTCAATcc                                                                     >  1:77539/1‑140 (MQ=255)                                                                         atcGCCAAACGGCAAAAGTTATAGCGAACGGTTAGATTCGGTGCGTACAGAAAAGCAATTGAGCGAGATTTTTGACGATCTGATCAACATGGTGCCAATGGGGCAGACGCTGTTTGGTTCGCTCAATCCGGTGCGCACCg                                                          >  1:417650/1‑140 (MQ=255)                                                                             ccAAACGGCAAAAGTTATAGCGAACGGTTAGATTCGGTGCGTACAGAAAAGCAATTGAGCGAGATTTTTGACGATCTGATCAACATGGTGCCAATGGGGCAGACGCTGTTTGGTTCGCTCAATCCGGTGCGCACCGGTgg                                                      >  1:252602/1‑140 (MQ=255)                                                                               aaaCGGCAAAAGTTATAGCGAACGGTTAGATTCGGTGCGTACAGAAAAGCAATTGAGCGAGATTTTTGACGATCTGATCAACATGGTGCCAATGGGGCAGACGCTGTTTGGTTCGCTCAATCCGGTGCGCACCGGTGGGc                                                    <  2:1434736/140‑1 (MQ=255)                                                                                    gCAAAAGTTATAGCGAACGGTTAGATTCGGTGCGTACAGAAAAGCAATTGAGCGAGATTTTTGACGATCTGATCAACATGGTGCCAATGGGGCAGACGCTGTTTGGTTCGCTCAATCCGGTGCGCACCGGTGGGCCGATg                                               <  2:955351/140‑1 (MQ=255)                                                                                              taGCGAACGGTTAGATTCGGTGCGTACAGAAAAGCAATTGAGCGAGATTTTTGACGATCTGATCAACATGGTGCCAATGGGGCAGACGCTGTTTGGTTCGCTCAATCCGGTGCGCACCGGTGGGCCGATGCAGGTCAGCa                                     <  1:971371/140‑1 (MQ=255)                                                                                                   aaCGGTTAGATTCGGTGCGTACAGAAAAGCAATTGAGCGAGATTTTTGACGATCTGATCAACATGGTGCCAATGGGGCAGACGCTGTTTGGTTCGCTCAATCCGGTGCGCACCGGTGGGCCGATGCAGGTCAGCATTGCt                                >  2:112533/1‑140 (MQ=255)                                                                                                   aaCGGTTAGATTCGGTGCGTACAGAAAAGCAATTGAGCGAGATTTTTGACGATCTGATCAACATGGTGCCAATGGGGCAGACGCTGTTTGGTTCGCTCAATCCGGTGCGCACCGGTGGGCCGATGCAGGTCAGCATTGCt                                >  1:415735/1‑140 (MQ=255)                                                                                                    aCGGTTAGATTCGGTGCGTACAGAAAAGCAATTGAGCGAGATTTTTGACGATCTGATCAACATGGTGCCAATGGGGCAGACGCTGTTTGGTTCGCTCAATCCGGTGCGCACCGGTGGGCCGATGCAGGTCAGCATTGCtt                               <  2:252602/140‑1 (MQ=255)                                                                                                     cGGTTAGATTCGGTGCGTACAGAAAAGCAATTGAGCGAGATTTTTGACGATCTGATCAACATGGTGCCAATGGGGCAGACGCTGTTTGGTTCGCTCAATCCGGTGCGCACCGGTGGGCCGATGCAGGTCAGCATTGCttt                              >  1:1191440/1‑140 (MQ=255)                                                                                                      ggTTAGATTCGGTGCGTACAGAAAAGCAATTGAGCGAGATTTTTGACGATCTGATCAACATGGTGCCAATGGGGCAGACGCTGTTTGGTTCGCTCAATCCGGTGCGCACCGGTGGGCCGATGCAGGTCAGCATTGCtttt                             >  1:598902/1‑140 (MQ=255)                                                                                                       gTTAGATTCGGTGCGTACAGAAAAGCAATTGAGCGAGATTTTTGACGATCTGATCAACATGGTGCCAATGGGGCAGACGCTGTTTGGTTCGCTCAATCCGGTGCGCACCGGTGGGCCGATGCATGTCAGCATTGCTTTTg                            <  2:907927/140‑1 (MQ=255)                                                                                                       gTTAGATTCGGTGCGTACAGAAAAGCAATTGAGCGAGATTTTTGACGATCTGATCAACATGGTGCCAATGGGGCAGACGCTGTTTGGTTCGCTCAATCCGGTGCGCACCGGTGGGCCGATGCAGGTCAGCATTGCTTTTg                            <  1:252440/140‑1 (MQ=255)                                                                                                       gTTAGATTCGGTGCGTACAGAAAAGCAATTGAGCGAGATTTTTGACGATCTGATCAACATGGTGCCAATGGGGCAGACGCTGTTTGGTTCGCTCAATCCGGTGCGCACCGGTGGGCCGATGCAGGTCAGCATTGCTTTTg                            <  2:77539/140‑1 (MQ=255)                                                                                                         tAGATTCGGTGCGTACAGAAAAGCAATTGAGCGAGATTTTTGACGATCTGATCAACATGGTGCCAATGGGGCAGACGCTGTTTGGTTCGCTCAATCCGGTGCGCACCGGTGGGCCGATGCAGGTCAGCATTGCTTTTGcc                          <  2:969156/140‑1 (MQ=255)                                                                                                          aGATTCGGTGCGTACAGAAAAGCAATTGAGCGAGATTTTTGACGATCTGATCAACATGGTGCCAATGGGGCAGACGCTGTTTGGTTCGCTCAATCCGGTGCGCACCGGTGGGCCGATGCAGGTCAGCATTGCTTTTGCCg                         <  2:86450/140‑1 (MQ=255)                                                                                                            aTTCGGTGCGTACAGAAAAGCAATTGAGCGAGATTTTTGACGATCTGATCAACATGGTGCCAATGGGGCAGACGCTGTTTGGTTCGCTCAATCCGGTGCGCACCGGTGGGCCGATGCAGGTCAGCATTGCTTTTGCCGaa                       <  1:833905/140‑1 (MQ=255)                                                                                                            aTTCGGTGCGTACAGAAAAGCAATTGAGCGAGATTTTTGACGATCTGATCAACATGGTGCCAATGGGGCAGACGCTGTTTGGTTCGCTCAATCCGGTGCGCACCGGTGGGCCGATGCAGGTCAGCATTGCTTTTGCCGaa                       <  2:417650/140‑1 (MQ=255)                                                                                                            aTTCGGTGCGTACAGAAAAGCAATTGAGCGAGATTTTTGACGATCTGATCAACATGGTGCCAATGGGGCAGACGCTGTTTGGTTCGCTCAATCCGGTGCGCACCGGTGGGCCGATGCAGGTCAGCATTGCTTTTGCCGaa                       <  1:1248727/140‑1 (MQ=255)                                                                                                            aTTCGGTGCGTACAGAAAAGCAATTGAGCGAGATTTTTGACGATCTGATCAACATGGTGCCAATGGGGCAGACGCTGTTTGGTTCGCTCAATCCGGTGCGCACCGGTGGGCCGATGCAGGTCAGCATTGCTTTTGCCGaa                       <  1:305220/140‑1 (MQ=255)                                                                                                              tCGGTGCGTACAGAAAAGCAATTGAGCGAGATTTTTGACGATCTGATCAACATGGTGCCAATGGGGCAGACGCTGTTTGGTTCGCTCAATCCGGTGCGCACCGGTGGGCCGATGCAGGTCAGCATTGCTTTTGCCGAACa                     <  1:491871/140‑1 (MQ=255)                                                                                                                ggTGCGTACAGAAAAGCAATTGAGCGAGATTTTTGACGATCTGATCAACATGGTGCCAATGGGGCAGACGCTGTTTGGTTCGCTCAATCCGGTGCGCACCGGTGGGCCGATGCAGGTCAGCATTGCTTTTGCCGAACAGc                   <  1:1077145/140‑1 (MQ=255)                                                                                                                ggTGCGTACAGAAAAGCAATTGAGCGAGATTTTTGACGATCTGATCAACATGGTGCCAATGGGGCAGACGCTGTTTGGTTCGCTCAATCCGGTGCGCACCGGTGGGCCGATGCAGGTCAGCATTGCTTTTGCCGAACAGc                   <  2:1109440/140‑1 (MQ=255)                                                                                                                ggTGCGTACAGAAAAGCAATTGAGCGAGATTTTTGACGATCTGATCAACATGGTGCCAATGGGGCAGACGCTGTTTGGTTCGCTCAATCCGGTGCGCACCGGTGGGCCGATGCAGGTCAGCATTGCTTTTGCCGAACAGc                   <  1:523312/140‑1 (MQ=255)                                                                                                                ggTGCGTACAGAAAAGCAATTGAGCGAGATTTTTGACGATCTGATCAACATGGTGCCAATGGGGCAGACGCTGTTTGGTTCGCTCAATCCGGTGCGCACCGGTGGGCCGATGCAGGTCAGCATTGCTTTTGCCGAACAGc                   <  2:361234/140‑1 (MQ=255)                                                                                                                ggTGCGTACAGAAAAGCAATTGAGCGAGATTTTTGACGATCTGATCAACATGGTGCCAATGGGGCAGACGCTGTTTGGTTCGCTCAATCCGGTGCGCACCGGTGGGCCGATGCAGGTCAGCATTGCTTTTGCCGAACAGc                   <  2:656174/140‑1 (MQ=255)                                                                                                                 gTGCGTACAGAAAAGCAATTGAGCGAGATTTTTGACGATCTGATCAACATGGTGCCAATGGGGCAGACGCTGTTTGGTTCGCTCAATCCGGTGCGCACCGGTGGGCCGATGCAGGTCAGCATTGCTTTTGCCGAACAGCa                  >  1:509653/1‑140 (MQ=255)                                                                                                                 gTGCGTACAGAAAAGCAATTGAGCGAGATTTTTGACGATCTGATCAACATGGTGCCAATGGGGCAGACGCTGTTTGGTTCGCTCAATCCGGTGCGCACCGGTGGGCCGATGCAGGTCAGCATTGCTTTTGCCGAACAGCa                  >  2:584580/1‑140 (MQ=255)                                                                                                                 gTGCGTACAGAAAAGCAATTGAGCGAGATTTTTGACGATCTGATCAACATGGTGACAATGGTGCAGAAGCTGTTTGGTTCGCTCAATCCGGTGCGCACCGGTGGGCCGATGCAGGTCAGCATTGCTTTTGCCGAACAGCa                  >  1:1054011/1‑140 (MQ=255)                                                                                                                  tGCGTACAGAAAAGCAATTGAGCGAGATTTTTGACGATCTGATCAACATGGTGCCAATGGGGCAGACGCTGTTTGGTTCGCTCAATCCGGTGCGCACCGGTGGGCCGATGCAGGTCAGCATTGCTTTTGCCGAACAGCAt                 >  1:127573/1‑140 (MQ=255)                                                                                                                  tGCGTACAGAAAAGCAAATGAGCGAGATTTTTGACGATCTGATCAACATGGTGCCAATGGGGCAGACGCTGTTTGGTTCGCTCAATCCGGTGCGCACCGGTGGGCCGATGCAGGTCAGCATTGCTTTTGCCGAACAGCAt                 >  1:1097605/1‑140 (MQ=255)                                                                                                                      tACAGAAAAGCAATTGAGCGAGATTTTTGACGATCTGATCAACATGGTGCCAATGGGGCAGACGCTGTTTGGTTCGCTCAATCCGGTGCGCACCGGTGGGCCGATGCAGGTCAGCATTGCTTTTGCCGAACAGCATACCa             <  2:22973/140‑1 (MQ=255)                                                                                                                              aGCAATTGAGCGAGATTTTTGACGATCTGATCAACATGGTGCCAATGGGGCAGACGCTGTTTGGTTCGCTCAATCCGGTGCGCACCGGTGGGCCGATGCAGGTCAGCATTGCTTTTGCCGAACAGCATACCAAAGGGTAt     >  2:533645/1‑140 (MQ=255)                                                                                                                               gCAATTGAGCGAGATTTTTGACGATCTGATCAACATGGTGCCAATGGGGCAGACGCTGTTTGGTTCGCTCAATCCGGTGCGCACCGGTGGGCCGATGCAGGTCAGCATTGCTTTTGCCGAACAGCATACCAAAGGGTATc    >  1:4890/1‑140 (MQ=255)                                                                                                                                cAATTGAGCGAGATTTTTGACGATCTGATCAACATGGTGCCAATGGGGCAGACGCTGTTTGGTTCGCTCAATCCGGTGCGCACCGGTGGGCCGATGCAGGTCAGCATTGCTTTTGCCGAACAGCATACCAAAGGGTATcc   >  2:60404/1‑140 (MQ=255)                                                                                                                                 aaTTGAGCGAGATTTTTGACGATCTGATCAACATGGTGCCAATGGGGCAGACGCTGTTTGGTTCGCTCAATCTGGTGCGCACCGGTGGGCCGATGCAGGTCAGCATTGCTTTTGCCGAACAGCATACCAAAGGGTATCCg  <  2:1133889/140‑1 (MQ=255)                                                                                                                                          |                                                                                                                                   GCAAGAGATTGACCGTCGTGCTGAACGGATGCACATTCCTGCTTTTCTGGTTCATACCGCGCTAAAAATCAAATCGCCAAACGGCAAAAGTTATAGCGAACGGTTAGATTCGGTGCGTACAGAAAAGCAATTGAGCGCGATTTTTGACGATCTGATCAACATGGTGCCAATGGGGCAGACGCTGTTTGGTTCGCTCAATCCGGTGCGCACCGGTGGGCCGATGCAGGTCAGCATTGCTTTTGCCGAACAGCATACCAAAGGGTATCCG  >  NC_000913/398180‑398447

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 26 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑