Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	1,284,167	G→T	L188L (CTG→CTT)	narH →	nitrate reductase A subunit beta

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,284,167	0	G	T	100.0%	251.1 / NA	70	L188L (CTG→CTT)	narH	nitrate reductase A subunit beta
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base T (41/29);  total (41/29)

GGAAGATGACCTGGGCGGTGAGTTTGACAAACTGGCGAAAGACAAGAACTTCGACAACATCCAGAAGGCGATGTATAGCCAGTTCGAAAACACCTTCATGATGTATTTGCCGCGCCTGTGCGAACACTGCCTGAACCCGGCATGTGTGGCGACCTGCCCGAGCGGTGCGATTTACAAGCGTGAAGAAGATGGCATCGTCCTGATCGACCAGGATAAATGCCGTGGCTGGCGTATGTGCATCACTGGATGCCCGTACAAAAAAATCTACT  >  NC_000913/1284035‑1284303                                                                                                                                     |                                                                                                                                         ggAAGATGACCTGGGCGGTGAGTTTGACAAACTGGCGAAAGACAAGAACTTCGACAACATCCAGAAGGCGATGTATAGCCAGTTCGAAAACACCTTCATGATGTATTTGCCGCGCCTGTGCGAACACTGCCTTAACCCgg                                                                                                                                   >  2:98690/1‑140 (MQ=255) ggAAGATGACCTGGGCGGTGAGTTTGACAAACTGGCGAAAGACAAGAACTTCGACAACATCCAGAAGGCGATGTATAGCCAGTTCGAAAACACCTTCATGATGTATTTGCCGCGCCTGTGCGAACACTGCCTTAACCCgg                                                                                                                                   >  2:700872/1‑140 (MQ=255)  gAAGATGACCTGGGCGGTGAGTTTGACAAACTGGCGAAAGACAAGAACTTCGACAACATCCAGAAGGCGATGTATAGTCAGTTCGAAAACACCTTCATGATGTATTTGCCGCGCCTGTGCGAACACTGCCTTAACCCGGc                                                                                                                                  >  1:637517/1‑140 (MQ=255)   aaGATGACCTGGGCGGTGAGTTTGACAAACTGGCGAAAGACAAGAACTTCGACAACATCCAGAAGGCGATGTATAGCCAGTTCGAAAACACCTTCATGATGTATTTGCCGCGCCTGTGCGAACACTGCCTTAACCCGGCa                                                                                                                                 <  1:93432/140‑1 (MQ=255)   aaGATGACCTGGGCGGTGAGTTTGACAAACTGGCGAAAGACAAGAACTTCGACAACATCCAGAAGGCGATGTATAGCCAGTTCGAAAACACCTTCATGATGTATTTGCCGCGCCTGTGCGAACACTGCCTTAACCCGGCa                                                                                                                                 <  1:92353/140‑1 (MQ=255)      aTGACCTGGGCGGTGAGTTTGACAAACTGGCGAAAGACAAGAACTTCGACAACATCCAGAAGGCGATGTATAGCCAGTTCGAAAACACCTTCATGATGTATTTGCCGCGCCTGTGCGAACACTGCCTTAACCCGGCAt                                                                                                                                >  1:1294010/1‑138 (MQ=255)      aTGACCTGGGCGGTGAGTTTGACAAACTGGCGAAAGACAAGAACTTCGACAACATCCAGAAGGCGATGTATAGCCAGTTCGAAAACACCTTCATGATGTATTTGCCGCGCCTGTGCGAACACTGCCTTAACCCGGCAt                                                                                                                                <  2:1294010/138‑1 (MQ=255)       tGACCTGGGCGGTGAGTTTGACAAACTGGCGAAAGACAAGAACTTCGACAACATCCAGAAGGCGATGTATAGCCAGTTCGAAAACACCTTCATGATGTATTTGCCGCGCCTGTGCGAACACTGCCTTAACCCGGCAtgtg                                                                                                                             >  2:1064349/1‑140 (MQ=255)        gACCTGGGCGGTGAGTTTGACAAACTGGCGAAAGACAAGAACTTCGACAACATCCAGAAGGCGATGTATAGCCAGTTCGAAAACACCTTCATGATGTATTTGCCGCGCCTGTGCGAACACTGCCTTAACCCGGCAtgtgt                                                                                                                            <  2:856966/140‑1 (MQ=255)           cTGGGCGGTGAGTTTGACAAACTGGCGAAAGACAAGAACTTCGACAACATCCAGAAGGCGATGTATAGCCAGTTCGAAAACACCTTCATGATGTATTTGCCGCGCCTGTGCGAACACTGCCTTAACCCGGCATGTGTGGc                                                                                                                         >  2:751190/1‑140 (MQ=255)                   tGAGTTTGACAAACTGGCGAAAGACAAGAACTTCGACAACATCCTGAAGGCGATGTATAGCCAGTTCGAAAACACCTTCATGATGTATTTGCCGCGCCTGTGCGAACACTGCCTTAACCCGGCATGTGTGGCGACCTGcc                                                                                                                 >  1:383749/1‑140 (MQ=255)                   tGAGTTTGACAAACTGGCGAAAGACAAGAACTTCGACAACATCCAGAAGGCGATGTATAGCCAGTTCGAAAACACCTTCATGATGTATTTGCCGCGCCTGTGCGAACACTGCCTTAACCCGGCATGTGTGGCGACCTGcc                                                                                                                 >  1:923680/1‑140 (MQ=255)                   tGAGTTTGACAAACTGGCGAAAGACAAGAACTTCGACAACATCCAGAAGGCGATGTATAGCCAGTTCGAAAACACCTTCATGATGTATTTGCCGCGCCTGTGCGAACACTGCCTTAACCCGGCATGTGTGGCGACCTGcc                                                                                                                 >  2:1142909/1‑140 (MQ=255)                   tGAGTTTGACAAACTGGCGAAAGACAAGAACTTCGACAACATCCAGAAGGCGATGTATAGCCAGTTCGAAAACACCTTCATGATGTATTTGCCGCGCCTGTGCGAACACTGCCTTAACCCGGCATGTGTGGCGACCTGcc                                                                                                                 >  2:955970/1‑140 (MQ=255)                       tttGACAAACTGGCGAAAGACAAGAACTTCGACAACATCCAGAAGGCGATGTATAGCCAGTTCGAAAACACCTTCATGATGTATTTGCCGCGCCTGTGCGAACACTGCCTTAACCCGGCATGTGTGGCGACCTGCCCGAg                                                                                                             <  1:1142909/140‑1 (MQ=255)                       tttGACAAACTGGCGAAAGACAAGAACTTCGACAACATCCAGAAGGCGATGTATAGCCAGTTCGAAAACACCTTCATGATGTATTTGCCGCGCCTGTGCGAACACTGCCTTAACCCGGCATGTGTGGCGACCTGCCCGAg                                                                                                             <  2:923680/140‑1 (MQ=255)                            cAAACTGGCGAAAGACAAGAACTTCGACAACATCGAGAAGGCGATGTATAGCCAGTTCGAAAACACCTTCATGATGTATTTGCCGCGCCTGTGCGAACACTGCCTTAACCCGGCATGTGTGGCGACCTGCCCGAGCGGTg                                                                                                        <  2:251829/140‑1 (MQ=255)                            cAAACTGGCGAAAGACAAGAACTTCGACAACATCCAGAAGGCGATGTATAGCCAGTTCGAAAACCCGTTCATGATGTATTTGCCGCGCCTGTGCGAACACTGCCTTAACCCGGCATGTGTGGCGACCTGCCCGAGCGGTg                                                                                                        <  1:1104601/140‑1 (MQ=255)                            cAAACTGGCGAAAGACAAGAACTTCGACAACATCCAGAAGGCGATGTATAGCCAGTTCGAAAACACCTTCATGATGTATTTGCCGCGCCTGTGCGAACACTGCCTTAACCCGGCATGTGTGGCGACCTGCCCGAGCGGTg                                                                                                        <  2:1188056/140‑1 (MQ=255)                               aCTGGCGAAAGACAAGAACTTCGACAACATCCAGAAGGCGATGTATAGCCAGTTCGAAAACACCTTCATGATGTATTTGCCGCGCCTGTGCGAACACTGCCTTAACCCGGCATGTGTGGCGACCTGCCCGAGCGGTg                                                                                                        >  2:1338414/1‑137 (MQ=255)                               aCTGGCGAAAGACAAGAACTTCGACAACATCCAGAAGGCGATGTATAGCCAGTTCGAAAACACCTTCATGATGTATTTGCCGCGCCTGTGCGAACACTGCCTTAACCCGGCATGTGTGGCGACCTGCCCGAGCGGTg                                                                                                        <  1:1338414/137‑1 (MQ=255)                                    cGAAAGACAAGAACTTCGACAACATCCAGAAGGCGATGTATAGCCAGTTCGAAAACACCTTCATGATGTATTTGCCGCGCCTGTGCGAACACTGCCTTAACCCGGCATGTGTGGCGACCTGCCCGAGCGGTGCGATTTAc                                                                                                >  2:1366186/1‑140 (MQ=255)                                    cGAAAGACAAGAACTTCGACAACATCCAGAAGGCGATGTATAGCCAGTTCGAAAACACCTTCATGATGTATTTGCCGCGCCTGTGCGAACACTGCCTTAACCCGGCATGTGTGGCGACCTGCCCGAGCGGTGCGATTTAc                                                                                                >  2:209855/1‑140 (MQ=255)                                      aaaGACAAGAACTTCGACAACATCCAGAAGGCGATGTATAGCCAGTTCGAAAACACCTTCATGATGTATTTGCCGCGCCTGTGCGAACACTGCCTTAACCCGGCATGTGTGGCGACCTGCCCGAGCGGTGCGATTTACaa                                                                                              <  1:955970/140‑1 (MQ=255)                                        aGACAAGAACTTCGACAACATCCAGAAGGCGATGTATAGCCAGTTCGAAAACACCTTCATGATGTATTTGCCGCGCCTGTGCGAACACTGCCTTAACCCGGCATGTGTGGCGACCTGCCCGAGCGGTGCGATTTACAAGc                                                                                            <  1:98690/140‑1 (MQ=255)                                           cAAGAACTTCGACAACATCCAGAAGGCGATGTATAGCCAGTTCGAAAACACCTTCATGATGTATTTGCCGCGCCTGTGCGAACACTGCCTTAACCCGGCATGTGTGGCGACCTGCCCGAGCGGTGCGATTTACAAGCGTg                                                                                         <  1:304026/140‑1 (MQ=255)                                             aGAACTTCGACAACATCCAGAAGGCGATGTATAGCCAGTTCGAAAACACCTTCATGATGTATTTGCCGCGCCTGTGCGAACACTGCCTTAACCCGGCATGTGTGGCGACCTGCCCGAGCGGTGCGATTTACAAGCGTgaa                                                                                       <  1:1017291/140‑1 (MQ=255)                                                 cTTCGACAACATCCTGAAGGCGATGTATAGCCAGTTCGAAAACACCTTCATGATGTATTTGCCGCGCCTGTGCGAACACTGCCTTAACCCGGCATGTGTGGCGACCTGCCCGAGCGGTGCGATTTACAAGCGTGaagaag                                                                                   <  2:383749/140‑1 (MQ=255)                                                    cGACAACATCCAGAAGGCGATGTATAGCCAGTTCGAAAACACCTTCATGATGTATTTGCCGCGCCTGTGCGAACACTGCCTTAACCCGGCATGTGTGGCGACCTGCCCGAGCGGTGCGATTTACAAGCGTGAAGAAGATg                                                                                >  1:967180/1‑140 (MQ=255)                                                    cGACAACATCCAGAAGGCGATGTATAGCCAGTTCGAAAACACCTTCATGATGTATTTGCCGCGCCTGTGCGAACACTGCCTTAACCCGGCATGTGTGGCGACCTGCCCGAGCGGTGCGATTTACAAGCGTGAAGAAGATg                                                                                >  1:144268/1‑140 (MQ=255)                                                      acaacaTCCAGAAGGCGATGTATAGCCAGTTCGAAAACACCTTCATGATGTATTTGCCGCGCCTGTGCGAACACTGCCTTAACCCGGCATGTGTGGCGACCTGCCCGAGCGGTGCGATTTACAAGCGTGAAGAAGATGGc                                                                              <  1:209855/140‑1 (MQ=255)                                                       caacaTCCAGAAGGCGATGTATAGCCAGTTCGAAAACACCTTCATGATGTATTTGCCGCGCCTGTGCGAACACTGCCTTAACCCGGCATGTGTGGCGACCTGCCCGAGCGGTGCGATTTACAAGCGTGAAGAAGATGGCa                                                                             <  2:959394/140‑1 (MQ=255)                                                       caacaTCCAGAAGGCGATGTATAGCCAGTTCGAAAACACCTTCATGATGTATTTGCCGCGCCTGTGCGAACACTGCCTTAACCCGGCATGTGTGGCGACCTGCCCGAGCGGTGCGATTTACAAGCGTGAAGAAGATGGCa                                                                             <  2:967180/140‑1 (MQ=255)                                                         acaTCCAGAAGGCGATGTATAGCCAGTTCGAAAACACCTTCATGATGTATTTGCCGCGCCTGTGCGACCACTGCCTTAACCCGGCATGTGTGGCGACCTGCCCGAGCGGTGCGATTTACAAGCGTGAAGAAGATGGCATc                                                                           >  2:901259/1‑140 (MQ=255)                                                                     cGATGTATAGCCAGTTCGAAAACACCTTCATGATGTATTTGCCGCGCCTGTGCGAACACTGCCTTAACCCGGTATGTGTGGCGACCTGCCCGAGCGGTGCGATTTACAAGCGTGAAGAAGATGGCATCGTCCTGATCGAc                                                               >  2:198632/1‑140 (MQ=255)                                                                     cGATGTATAGCCAGTTCGAAAACACCTTCATGATGTATTTGCCGCGCCTGTGCGAACACTGCCTTAACCCGGCATGTGTGGCGACCTGCCCGAGCGGTGCGATTTACAAGCGTGAAGAAGATGGCATCGTCCTGATCGAc                                                               <  1:1366186/140‑1 (MQ=255)                                                                       aTGTATAGCCAGTTCGAAAACACCTTCATGATGTATTTGCCGCGCCTGTGCGAACACTGCCTTAACCCGGCATGTGTGGCGACCTGCCCGAGCGGTGCGATTTACAAGCGTGAAGAAGATGGCATCGTCCTGATCGACCa                                                             >  1:468823/1‑140 (MQ=255)                                                                        tGTATAGCCAGTTCGAAAACACCTTCATGATGTATTTGCCGCGCCTGTGCGAACACTGCCTTAACCCGGCATGTGTGGCGACCTGCCCGAGCGGTGCGATTTACAAGCGTGAAGAAGATGGCATCGTCCTGATCGACCAg                                                            >  2:378812/1‑140 (MQ=255)                                                                        tGTATAGCCAGTTCGAAAACACCTTCATGATGTATTTGCCGCGCCTGTGCGAACACTGCCTTAACCCGGCATGTGTGGCGACCTGCCCGAGCGGTGCGATTTACAAGCGTGAAGAAGATGGCATCGTCCTGATCGACCAg                                                            >  2:904484/1‑140 (MQ=255)                                                                                  gTTCGAAAACACCTTCATGATGTATTTGCCGCGCCTGTGCGAACACTGCCTTAACCCGGCATGTGTGGCGACCTGCCCGAGCGGTGTGATTTACAAGCGTGAAGAAGATGGCATCGTCCTGATCGACCAGGATAAATGcc                                                  >  2:1189199/1‑140 (MQ=255)                                                                                  gTTCGAAAACACCTTCATGATGTATTTGCCGCGCCTGTGCGAACACTGCCTTAACCCGGCATGTGTGGCGACCTGCCCGAGCGGTGCGATTTACAAGCGTGAAGAAGATGGCATCGTCCTGATCGACCAGGATAAATGcc                                                  >  2:1417919/1‑140 (MQ=255)                                                                                   ttCGAAAACACCTTCATGATGTATTTGCCGTGCCTGTGCGAACACTGCCTTAACCCGGCATGTGTGGCGACCTGCCCGAGCGGTGCGATTTACAAGCGTGAAGAAGATGGCATCGTCCTGATCGAcc                                                              <  1:648207/127‑1 (MQ=255)                                                                                   ttCGAAAACACCTTCATGATGTATTTGCCGTGCCTGTGCGAACACTGCCTTAACCCGGCATGTGTGGCGACCTGCCCGAGCGGTGCGATTTACAAGCGTGAAGAAGATGGCATCGTCCTGATCGAcc                                                              >  2:648207/1‑127 (MQ=255)                                                                                   ttCGAAAACACCTTCATGATGTATTTGCCGCGTCTGTGCGAACACTGCCTTAACCCGGCATGTGTGGCGACCTGCCCGAGCGGTGCGATTTACAAGCGTGAAGAAGATGGCATCGTCCTGATCGACCAGGATAAATGCCg                                                 >  1:860182/1‑140 (MQ=255)                                                                                   ttCGAAAACACCTTCATGATGTATTTGCCGCGCCTGTGCGAACACTGCCTTAACCCGGCATGTGTGGCGACCTGCCCGAGCGGTGCGATTTACAAGCGTGAAGAAGATGGCATCGTCCTGATCGACCAg                                                            <  1:833456/129‑1 (MQ=255)                                                                                   ttCGAAAACACCTTCATGATGTATTTGCCGCGCCTGTGCGAACACTGCCTTAACCCGGCATGTGTGGCGACCTGCCCGAGCGGTGCGATTTACAAGCGTGAAGAAGATGGCATCGTCCTGATCGACCAg                                                            >  2:833456/1‑129 (MQ=255)                                                                                   ttCGAAAACACCTTCATGATGTATTTGCCGCGCCTGTGCGAACACTGCCTTAACCCGGCATGTGTGGCGACCTGCCCGAGCGGTGCGATTTACAAGCGTGAAGAAGATGGCATCGTCCTGATCGACCAGGATAAATGCCg                                                 >  1:1017865/1‑140 (MQ=255)                                                                                   ttCGAAAACACCTTCATGATGTATTTGCCGCGCCTGTGCGAACACTGCCTTAACCCGGCATGTGTGGCGACCTGCCCGAGCGGTGCGATTTACAAGCGTGAAGAAGATGGCATCGTCCTGATCGACCAGGATAAATGCCg                                                 >  1:346464/1‑140 (MQ=255)                                                                                   ttCGAAAACACCTTCATGATGTATTTGCCGCGCCTGTGCGAACACTGCCTTAACCCGGCATGTGTGGCGACCTGCCCGAGCGGTGCGATTTACAAGCGTGAAGAAGATGGCATCGTCCTGATCGACCAGGATAAATGCCg                                                 >  2:1289134/1‑140 (MQ=255)                                                                                    tCGAAAACACCTTCATGATGTATTTGCCGCGCCTGTGCGAACACTGCCTTAACCCGGCATGTGTGGCGACCTGCCCGAGCGGTGCGATTTACAAGCGTGAAGAAGATGGCATCGTCCTGATCGACCAGGATAAATGCCGt                                                >  2:1280723/1‑140 (MQ=255)                                                                                      gAAAACACCTTCATGATGTATTTGCCGCGCCTGTGCGAACACTGCCTTAACCCGGCATGTGTGGCGACCTGCCCGAGCGGTGCGATTTACAAGCGTGAAGAAGATGGCATCGTCCTGATCGACCAGGATAAATGCCGtgg                                              <  1:751190/140‑1 (MQ=255)                                                                                             ccTTCATGATGTATTTGCCGCGCCTGTGCGAACACTGCCTTAACCCGGCATGTGTGGCGACCTGCCCGAGCGGTGCGATTTACAAGCGTGAAGAAGATGGCATCGTCCTGATCGACCAGGATAAATGCCGTGGCTGGCGt                                       <  1:813034/140‑1 (MQ=255)                                                                                                tCATGATGTATTTGCCGCGCCTGTGCGAACACTGCCTTAACCCGGCATGTGTGGCGACCTGCCCGAGCGGTGCGATTTACAAGCGTGAAGAAGATGGCATCGTCCTGATCGACCAGGATAAATGCCGtggctg                                           >  1:483481/1‑133 (MQ=255)                                                                                                tCATGATGTATTTGCCGCGCCTGTGCGAACACTGCCTTAACCCGGCATGTGTGGCGACCTGCCCGAGCGGTGCGATTTACAAGCGTGAAGAAGATGGCATCGTCCTGATCGACCAGGATAAATGCCGtggctg                                           <  2:483481/133‑1 (MQ=255)                                                                                                tCATGATGTATTTGCCGCGCCTGTGCGAACACTGCCTTAACCCGGCATGTGTGGCGACCTGCCCGAGCGGTGCGATTTACAAGCGTGAAGAAGATGGCATCGTCCTGATCGACCAGGATAAATGCCGTGGCTGGCGTAtg                                    <  1:1280723/140‑1 (MQ=255)                                                                                                 cATGATGTATTTGCCGCGCCTGTGCGAACACTGCCTTAACCCGGCATGTGTGGCGACCTGCCCGAGCGGTGCGATTTACAAGCGTGAAGAAGATGGCATCGTCCTGATCGACCAGGATAAATGCCGTGGCTGGCGTTtgt                                   <  2:1017865/140‑1 (MQ=255)                                                                                                 cATGATGTATTTGCCGCGCCTGTGCGAACACTGCCTTAACCCGGCATGTGTGGCGACCTGCCCGAGCGGTGCGATTTACAAGCGTGAAGAAGATGGCATCGTCCTGATCGACCAGGATAAATGCCGTGGCTGGCGTAtgt                                   >  1:285813/1‑140 (MQ=255)                                                                                                  atgatgTATTTGCCGCGCCTGTGCGAACACTGCCTTAACCCGGTATGTGTGGCGACCTGCCCGAGCGGTGCGATTTACAAGCGTGAAGAAGATGGCATCGTCCTGATCGACCAGGATAAATGCCGTGGCTGGCGTAtgtg                                  <  1:198632/140‑1 (MQ=255)                                                                                                  atgatgTATTTGCCGCGCCTGTGCGAACACTGCCTTAACCCGGCATGTGTGGCGACCTGCCCGAGCGGTGCGATTTACAAGCGTGAAGAAGATGGCATCGTCCTGATCGACCAGGATAAATGCCGTGGCTGGCGTAtgtg                                  <  1:378812/140‑1 (MQ=255)                                                                                                      tgTATTTGCCGCGCCTGTGCGAACACTGCCTTAACCCGGCATGTGTGGCGACCTGCCCGAGCGGTGCGATTTACAAGCGTGAAGAAGATGGCATCGTCCTGATCGACCAGGATAAATGCCGTGGCTGGCGTATGTGCATc                              >  1:685943/1‑140 (MQ=255)                                                                                                          tttGCCGCGCCTGTGCGAACACTGCCTTAACCCGGCATGTGTGGCGACCTGCCCGAGCGGTGCGATTTACAAGCGTGAAGAAGATGGCATCGTCCTGATCGACCAGGATAAATGCCGTGGCTGGCGTATGTGCATc                              <  1:1388269/136‑1 (MQ=255)                                                                                                          tttGCCGCGCCTGTGCGAACACTGCCTTAACCCGGCATGTGTGGCGACCTGCCCGAGCGGTGCGATTTACAAGCGTGAAGAAGATGGCATCGTCCTGATCGACCAGGATAAATGCCGTGGCTGGCGTATGTGCATCACTg                          >  1:390130/1‑140 (MQ=255)                                                                                                          tttGCCGCGCCTGTGCGAACACTGCCTTAACCCGGCATGGGTGGCGACCTGCCCGAGCGGTGCGATTTACAAGCGTGAAGAAGATGGCATCGTCCTGATCGACCAGGATAAATGCCGTGGCTGGCGTATGTGCATc                              >  2:1388269/1‑136 (MQ=255)                                                                                                               cgcgCCTGTGCGAACACTGCCTTAACCCGGCATGTGTGGCGACCTGCCCGAGCGGTGCGATTTACAAGCGTGAAGAAGATGGCATCGTCCTGATCGACCAGGATAAATGCCGTGGCTGGCGTATGTGCATCACTGGATGc                     >  1:601652/1‑140 (MQ=255)                                                                                                                 cgcCTGTGCGAACACTGCCTTAACCCGGCATGTGTGGCGACCTGCCCGAGCGGTGCGATTTACAAGCGTGAAGAAGATGGCATCGTCCTGATCGACCAGGATAAATGCCGTGGCTGGCGTATGTGCATCACTGGATGccc                   >  2:1390327/1‑140 (MQ=255)                                                                                                                 cgcCTGTGCGAACACTGCCTTAACCCGGCATGTGTGGCGACCTGCCCGAGCGGTGCGATTTACAAGCGTGAAGAAGATGGCATCGTCCTGATCGACCAGGATAAATGCCGTGGCTGGCGTATGTGCATCACTGGATGccc                   >  1:125456/1‑140 (MQ=255)                                                                                                                  gcCTGTGCGAACACTGCCTTAACCCGGCATGTGTGGCGACCTGCCCGAGCGGTGCGATTTACAAGCGTGAAGAAGATGGCATCGTCCTGATCGACCAGGATAAATGCCGTGGCTGGCGTATGTGCATCACTGGATGCCCg                  >  2:1337098/1‑140 (MQ=255)                                                                                                                    cTGTGCGAACACTGCCTTAACCCGGCATGTGTGGCGACCTGCCCGAGCGGTGCGATTTACAAGCGTGAAGAAGATGGCATCGTCCTGATCGACCAGGATAAATGCCGTGGCTGGCGTATGTGCATCACTGGATGCCCGTa                <  2:1101945/140‑1 (MQ=255)                                                                                                                         cGAACACTGCCTTAACCCGGCATGTGTGGCGACCTGCCCGAGCGGTGCGATTTACAAGCGTGAAGAAGATGGCATCGTCCTGATCGACCAGGATAAATGCCGTGGCTGGCGTATGTGCATCACTGGATGCCCGTACaaaa           <  2:601652/140‑1 (MQ=255)                                                                                                                              acTGCCTTAACCCGGCATGTGTGGCGACCTGCCCGAGCGGTGCGATTTACAAGCGTGAAGAAGATGGCATCGTCCTGATCGACCAGGATAAATGCCGTGGCTGGCGTATGTGCATCACTGGATGCCCGTACAAAAAAATc      >  1:1183473/1‑140 (MQ=255)                                                                                                                                tGCCTTAACCCGGCATGTGTGGCGACCTGCCCGAGCGGTGCGATTTACAAGCGTGAAGAAGATGGCATCGTCCTGATCGACCAGGATAAATGCCGTGGCTGGCGTATGTGCATCACTGGATGCCCGTACAAAAAAATCTa    >  1:306025/1‑140 (MQ=255)                                                                                                                                tGCCTTAACCCGGCATGTGTGGCGACCTGCCCGAGCGGTGCGATTTACAAGCGTGAAGAAGATGGCATCGTCCTGATCGACCAGGATAAATGCCGTGGCTGGCGTATGTGCATCACTGGATGCCCGTACAAAAAAATCTa    >  1:953303/1‑140 (MQ=255)                                                                                                                                tGCCTTAACCCGGCATGTGTGGCGACCTGCCCGAGCGGTGCGATTTACAAGCGTGAAGAAGATGGCATCGTCCTGATCGACCAGGATAAATGCCGTGGCTGGCGTATGTGCATCACTGGATGCCCGTACAAAAAAATCTa    >  1:577308/1‑140 (MQ=255)                                                                                                                                  ccTTAACCCGGCATGTGTGGCGACCTGCCCGAGCGGTGCGATTTACAAGCGTGAAGAAGATGGCATCGTCCTGATCGACCAGGATAAATGCCGTGGCTGGCGTATGTGCATCACTGGATGCCCGTACAAAAAAATCTACt  <  1:1390327/140‑1 (MQ=255)                                                                                                                                     |                                                                                                                                         GGAAGATGACCTGGGCGGTGAGTTTGACAAACTGGCGAAAGACAAGAACTTCGACAACATCCAGAAGGCGATGTATAGCCAGTTCGAAAACACCTTCATGATGTATTTGCCGCGCCTGTGCGAACACTGCCTGAACCCGGCATGTGTGGCGACCTGCCCGAGCGGTGCGATTTACAAGCGTGAAGAAGATGGCATCGTCCTGATCGACCAGGATAAATGCCGTGGCTGGCGTATGTGCATCACTGGATGCCCGTACAAAAAAATCTACT  >  NC_000913/1284035‑1284303

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 23 ≤ ATCG/ATCG < 31 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑