Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009133,556,2070TC30.8% 61.3 / 14.1 39S215G (AGT→GGT) ‡rtcARNA 3'‑terminal phosphate cyclase
Reads supporting (aligned to +/- strand):  ref base T (25/2);  new base C (0/12);  total (25/14)
Fisher's exact test for biased strand distribution p-value = 2.33e-08
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00
Rejected as consensus: Frequency below/above cutoff threshold.

GCACTGACGCGCTTTTCACCGACGACAAAAAAGCGTTCGGTGATATTTTCACTTTCGACTTCAAGCGAGACGGTATTACCCGGCCCCTGGTCGCGCGGCAGGTTATGAATATTCTGTTCATGCAGGGAAAA‑A‑CTCCCCGCCAGTGTAGCGATTTCACGCTCAGCAACATGGCGCGGCACACCAGCTAATAGAACTTCTCCACGCATCTGCACAATGTTCCCGCGCTCGCCAAGTTGCAAGGTGTTAAACGATGCCACGGGCGAGACTTCC  >  NC_000913/3556074‑3556343
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gCACTGACGCTTTTTTCACCGACGAAAAAAAAGCGTTCGGTGATTTTTTCACTTTCGACTTCAAGCGAGACGGTATTCCCCGGCCCCTGGCCGCGCGGCAGGTTATAAATATTCTGTTCATGCAGGAAAAA‑C‑CCCCCCGc                                                                                                                                    <  2:1663911/140‑1 (MQ=255)
   cTGACGCGCTTTTCACCGACGACAAAAAAGCGTCCGGTGATATTTTCACTTTCGACTTCAAGCGAGACGGTATTACCCGGCCCCTGGTCGCGCGGCAGGTTATGAATATTCTGTTCATGCAGGGAAAA‑A‑CTCCCCGCCAg                                                                                                                                 >  2:906107/1‑140 (MQ=255)
      aCGCGCTTTTCACCGACGACAAAAAAGCGTTCGGTGATATTTTCACTTTCGACTTCAAGCGAGACGGTATTACCCGGCCCCTGGTCGCGCGGCAGGTTATGAATATTCTGTTCATGCAGGGAAAA‑A‑CTCCCCGCCAgtgt                                                                                                                              >  2:157367/1‑140 (MQ=255)
      aCGCGCTTTTCACCGACGACAAAAAAGCGTTCGGTGATATTTTCACTTTCGACTTCAAGCGAGACGGTATTACCCGGCCCCTGGTCGCGCGGCAGGTTATGAATATTCTGTTCATGCAGGGAAAA‑A‑CTCCCCGCCAgtgt                                                                                                                              >  1:1886633/1‑140 (MQ=255)
                cACCGACGACAAAAAAGCGTTCGGTGATATTTTCACTTTCGACTTCAAGCGAGACGGTATTACCCGGCCCCTGGTCGCGCGGCAGGTTATGAATATTCTGTTCATGCAGGGAAAA‑A‑CTCCCCGCCAGTGTAGCGATTTCa                                                                                                                    >  1:754345/1‑140 (MQ=255)
                  ccGACGAAAAAAACGCGTTGGGTGATTTTTTCTCTTTCGACTTAAAGCGAGCCGTTATTCCCGGGCCCGGGGTCGCGCGGCAGTTTAGAAATTTTTTTTTCATGCAGGGAAAA‑C‑CCCCCCGCCAGTGTAGCGATTTCACg                                                                                                                  <  2:1100678/140‑1 (MQ=255)
                  ccGACGAAAAAAAAGCGTTGGGTGATTTTTTCCCTTTCGACTTCAAGCGAGACGGTATTCCCCGGCCCCTGGTCGCGCGGCAGGTTATGAATATTCTGTTCATGCAGGAAAAA‑C‑CCCCCCGCCAGTGTAGCGATTTCACg                                                                                                                  <  2:754345/140‑1 (MQ=255)
                  ccGACAAAAAAAAGGCGTGGGGTTATTTTTTCTTTTTCGTTTTAAAGCGAGCCGTTATTCCCCGCCCCCGGGCCGCGGGGGGGGTTATAAATTTTCTGTTCATGCAGGAAAAA‑C‑CCCCCCGCCAGTGTAGCGATTTCACg                                                                                                                  <  2:433010/140‑1 (MQ=255)
                              aaGCTTTCGGTGATATTTTCTTTTCCAAATTCAAGGGAGACGGTATCCCCCGGCCCCTGGCCGCGGGGCAGGTTATAAATTTTCTGTTCATGCAGGAAAAA‑T‑CCCCCCGCCAGTGTAGCGATTTCACGCTCAGCAACATg                                                                                                      <  2:478092/140‑1 (MQ=255)
                                 ctttCGGTGATTTTTTCTTTTTCGACTTAAAGCGAGAGGGTATTCCCCGCCCCCTGGTCGCGCGGCAGTTTATAAATTTTCTTTTCATGAAGGAAAAA‑C‑CCCCCCGCCAGTGTAGCGATTTCACGCTCAGCAACATGgcg                                                                                                   <  2:2090108/138‑1 (MQ=255)
                                   ttgggTGATTTTTTCTCTTTCGACTTCAAGCGAGACGGTATTCCCCGGCCCCTGGTCGCGCGGCAGTTTATAAATTTTCTGTTCATGCAGGAAAAA‑C‑CCCCCCGCCAGTGTAGCGATTTCACGCTCAGCAACATGGcgcg                                                                                                 <  2:1260734/137‑1 (MQ=255)
                                   ttCGGTGATATTTTCACTTTCGACTTCAAGCGAGACGGTATTACCCGGCCCCTGGTCGCGCGGCAGGTTATGAATATTCTGTTCATGCAGGGAAAA‑A‑CTCCCCGCCAGTGTAGCGATTTCACGCTCAGCAACATGGcgcg                                                                                                 >  1:2090108/1‑140 (MQ=255)
                                        tttttttttCCTTTTCGCTTTAAAGGGAGACGGTTTTCCCCGGCCCCTGGTCGCGCGGGGGGTTATAAATTTTCTGTTCATGCGGGAAAAA‑C‑CCCCCCGCCAGTGTAGCGATTTCACGCTCAGCAACATGGCGCGGcaca                                                                                            <  2:1481734/135‑1 (MQ=255)
                                        ttattttttCTTTTTCGACTTCAAGGGAGACGGTATTCCCCGGCCCGTGGTCGCGCGGCAGGTTATGAATTTTCTGTTCAGGCAGGAAAAA‑C‑CCCCCCGCCAGTGTAGCGATTTCACGCTCAGCAACATGGCGCGGcaca                                                                                            <  2:1886633/135‑1 (MQ=255)
                                                  aCTTTCGACTTCAAGCGAGACGGTATTACCCGGCCCCTGGTCGCGCGGCAGGTTATGAATATTCTGTTCATGCAGGGAAAA‑A‑CTCCCCGCCAGTGTAGCGATTTCACGCTCAGCAACATGGCGCGGCACACCAGCTAATa                                                                                  >  1:1481734/1‑140 (MQ=255)
                                                  aCTTTCGACTTCAAGCGAGACGGGATTACCCGGCCCCTGGTCGCGCGGCAGGTTATGAATATTCTGTTCATGCAGGGAAAA‑A‑CTCCCCGCCAGTGTAGCGATTTCACGCTCAGCAACATGGCGCGGCACACCAGCTAATa                                                                                  >  2:1175562/1‑140 (MQ=255)
                                                    tttCGACTTCAAGCGAGACGGTATTACCCGGCCCCTGGTCGCGCGGCAGGTTATGAATATTCTGTTCATGCAGGGAAAA‑A‑CTCCCCGCCAGTGTAGCGATTTCACGCTCAGCAACATGGCGCGGCACACCAGCTAATAGa                                                                                >  1:1953416/1‑140 (MQ=255)
                                                        gccTTAAAGCGAGACGGTTTTCCCCGGCCCCGGGCCGCGCGGCAGTTTATAAATTTTCTGTTCATGAAGGAAAAA‑C‑CCCCCCGCCAGTGTAGCGATTTCACGCTCAGCAACATGGCGCGGCACACCAGCTAATAGAACtt                                                                            <  2:1953416/138‑1 (MQ=255)
                                                             aaaGCGAGCCGGTATCCCCCGGCCCCGGGCCGCGCGGCAGTTTATGAATTTTCTTTTCATGAAGGAAAAA‑A‑CCCCCCGCCAGTGTAGCGATTTCACGCTCAGCAACATGGCGCGGCACACCAGCTAATAGAACTTCTCCa                                                                       <  2:2186867/139‑1 (MQ=255)
                                                                  gggaCGTTATCCCCCGGCCCCTGGCCGCGCGGAGGTTTATAAATTTTCTGTTCATGAAGGAAAAA‑C‑CCCCCCGCCAGTGTAGCGATTTCACGCTCAGCAACATGGCGCGGCACACCAGCTAATAGAACTTCTCCACGCAt                                                                  <  2:1765754/138‑1 (MQ=255)
                                                                  gagaCGTTATTCCCCGGCCCCTGGTCGCGCGGCAGGTTATGAATTTTCTGTTCATGCAGGAAAAA‑C‑CTCCCCGCCAGTGTAGCGATTTCACGCTCAGCAACATGGCGCGGCACACCAGCTAATAGAACTTCTCCACGCAt                                                                  <  1:781248/140‑1 (MQ=255)
                                                                    gaCGGTATTACCCGGCCCCTGGTCGCGCGGCAGGTTATGAATATTCTGTTCATGCAGGGAAAA‑A‑CTCCCCGCCAGTGTAGCGATTTCACGCTCAGCAACATGGCGCGGCACACCAGCTAATAGAACTTCTCCACGCATCt                                                                >  1:841846/1‑140 (MQ=255)
                                                                         tATTACCCGGCCCCTGGTCGCGCGGCAGGTTATGAATATTCTGTTCATGCAGGGAAAA‑A‑CTCCCCGCCAGTGTAGCGATTTCACGCTCAGCAACATGGCGCGGCACACCAGCTAATAGAACTTCTCCACGCATCTGcaca                                                           >  1:1011598/1‑140 (MQ=255)
                                                                                   ccccTGGTCGCGCGGCAGGTTATGAATATTCTGTTCATGCAGGGAAAA‑A‑CTCCCCGCCAGTGTAGCGATTTCACGCTCAGCAACATGGCGCGGCACACCAGCTAATAGAACTTCTCCACGCATCTGCACAATGTTCCcgc                                                 >  2:581725/1‑140 (MQ=255)
                                                                                   ccccTGGTCGCGCGGCAGGTTATGAATATTCTGTTCATGCAGGGAAAA‑A‑CTCCCCGCCAGTGTAGCGATTTCACGCTCAGCAACATGGCGCGGCACACCAGCTAATAGAACTTCTCCACGCATCTGCACAATGTTCCcgc                                                 >  2:1320941/1‑140 (MQ=255)
                                                                                          ccgcgcgGCAGGTTATAAATATTCTGTTCATGCAGGAAAAA‑C‑CCCCCCGCCAGTGTAGCGATTTCACGCTCAGCAACATGGCGCGGCACACCAGCTAATAGAACTTCTCCACGCATCTGCACAATGTTCCCGCGCTCGcc                                          <  1:157367/139‑1 (MQ=255)
                                                                                             cgcgGCAGGTTATGAATATTCTGTTCATGCAGGGAAAA‑A‑CTCCCCGCCAGTGTAGCGATTTCACGCTCAGCAACATGGCGCGGCACACCAGCTAATAGAACTTCTCCACGCATCTGCACAATGTTCCCGCGCTCGCCAAg                                       >  1:972162/1‑140 (MQ=255)
                                                                                             cgcgGCAGGTTATGAATATTCTGTTCATGCAGGGAAAA‑A‑CTCCCCGCCAGTGTAGCGATTTCACGCTCAGCAACATGGCGCGGCACACCAGCTAATAGAACTTCTCCACGCATCTGCACAATGTTCCCGCGCTCGCCAAg                                       >  1:2290065/1‑140 (MQ=255)
                                                                                             cgcgGCAGGTTATGAATATTCTGTTCATGCAGGGAAAA‑A‑CTCCCCGCCAGTGTAGCGATTTCACGCTCAGCAACATGGCGCGGCACACCAGCTAATAGAACTTCTCCACGCATCTGCACAATGTTCCCGCGCTCGCCAAg                                       >  1:1945858/1‑140 (MQ=255)
                                                                                             cgcgGCAGGTTATGAATATTCTGTTCATGCAGGGAAAA‑A‑CTCCCCGCCAGTGTAGCGATTTCACGCTCAGCAACATGGCGCGGCACACCAGCTAATAGAACTTCTCCACGCATCTGCACAATGTTCCCGCGCTCGCCAAg                                       >  1:1727876/1‑140 (MQ=255)
                                                                                                 gCAGGTTATGAATATTCTGTTCATGCAGGGAAAA‑A‑CTCCCCGCCAGTGTAGCGATTTCACGCTCAGCAACATGGCGCGGCACACCAGCTAATAGAACTTCTCCACGCATCTGCACAATGTTCCCGCGCTCGCCAAGTTGc                                   >  1:1232932/1‑140 (MQ=255)
                                                                                                  gagttaaTGAATATCTTGTAAATG‑AGGGAAAATC‑CCCCCCGCCAGTGTAGCGATTTCACGCTCAGCAACATGGCGCGGCACACCAGCTAATAGAACTTCTCCACGCATCTGCACAATGTTCCCGCGCTCGCCAAGTTGCa                                  <  2:264705/134‑1 (MQ=255)
                                                                                                  gaGGTTATGAATTTTCTGTTCAGGCAGGAAAAA‑C‑CCCCCCGCCAGTGTAGCGATTTCACGCTCAGCAACATGGCGCGGCACACCAGCTAATAGAACTTCTCCACGCATCTGCACAATGTTCCCGCGCTCGCCAAGTTGCa                                  <  2:1463125/139‑1 (MQ=255)
                                                                                                        aTGAATATTCTGTTCATGCAGGAAAAA‑C‑CCCCCCGCCAGTGTAGCGATTTCACGCTCAGCAACATGGCGCGGCACACCAGCTAATAGAACTTCTCCACGCATCTGCACAATGTTCCCGCGCTCGCCAAGTTGCAAGgtgt                            <  2:1011598/140‑1 (MQ=255)
                                                                                                                    ttCATGCAGGGAAAA‑A‑CTCCCCGCCAGTGTAGCGATTTCACGCTCAGCAACATGGCGCGGCACACCAGCTAATAGAACTTCTCCACGCATCTGCACAATGTTCCCGCGCTCGCCAAGTTGCAAGGTGTTAAACGATGCCa                >  1:708974/1‑140 (MQ=255)
                                                                                                                    ttCATGCAGGGAAAA‑A‑CTCCCCGCCAGTGTAGCGATTTCACGCTCAGCAACATGGCGCGGCACACCAGCTAATAGAACTTCTCCACGCATCTGCACAATGTTCCCGCGCTCGCCAAGTTGCAAGGTGTTAAACGATGCCa                >  2:329866/1‑140 (MQ=255)
                                                                                                                    ttCAGGGAGGAAAAA‑C‑CTCCCCGCCAGTGTAGCGATTTCACGCTCAGCAACATGGCGCGGCACACCAGCTAATAGAACTTCTCCACGCATCTGCACAATGTTCCCGCGCTCGCCAAGTTGCAAGGTGTTAAACGATGCCa                <  2:418363/140‑1 (MQ=255)
                                                                                                                      cATGCAGGGAAAA‑A‑CTCCCCGCCAGTGTAGCGATTTCACGCTCAGCAACATGGCGCGGCACACCAGCTAATAGAACTTCTCCACGCATCTGCACAATGTTCCCGCGCTCGCCAAGTTGCAAGGTGTTAAACGATGCCACg              >  1:1524510/1‑140 (MQ=255)
                                                                                                                       gtggaGGGAAAA‑ACCTCCCCGCCAGTGTAGCGATTTCACGCTCAGCAACATGGCGCGGCACACCAGCTAATAGAACTTCTCCACGCATCTGCACAATGTTCCCGCGCTCGCCAAGTTGCAAGGTGTTAAACGATGCCACg              <  2:1271307/136‑1 (MQ=255)
                                                                                                                       aTGCAGGGAAAA‑A‑CTCCCCGCCAGTGTAGCGATTTCACGCTCAGCAACATGGCGCGGCACACCAGCTAATAGAACTTCTCCACGCATCTGCACAATGTTCCCGCGCTCGCCAAGTTGCAAGGTGTTAAACGATGCCACgg             >  1:205406/1‑140 (MQ=255)
                                                                                                                        tGCAGGGAAAA‑A‑CTCCCCGCCAGTGTAGCGATTTCACGCTCAGCAACATGGCGCGGCACACCAGCTAATAGAACTTCTCCACGCATCTGCACAATGTTCCCGCGCTCGCCAAGTTGCAAGGTGTTAAACGATGCCACggg            >  1:2257562/1‑140 (MQ=255)
                                                                                                                           aGGGAAAA‑A‑CTCCCCGCCAGTGTAGCGATTTCACGCTCAGCAACATGGCGCGGCACACCAGCTAATAGAACTTCTCCACGCATCTGCACAATGTTCCCGCGCTCGCCAAGTTGCAAGGTGTTAAACGATGCCACGGGCga         >  2:903914/1‑140 (MQ=255)
                                                                                                                           aGGAAAAA‑C‑CCCCCCGCCAGTGTAGCGATTTCACGCTCAGCAACATGGCGCGGCACACCAGCTAATAGAACTTCTCCACGCATCTGCACAATGTTCCCGCGCTCGCCAAGTTGCAAGGTGTTAAACGATGCCACGGGCga         <  2:972162/140‑1 (MQ=255)
                                                                                                                             ggAAAA‑A‑CTCCCCGCCAGTGTAGCGATTTCACGCTCAGCAACATGGCGCGGCACACCAGCTAATAGAACTTCTCCACGCATCTGCACAATGTTCCCGCGCTCGCCAAGTTGCAAGGTGTTAAACGATGCCACGGGCgaga       >  2:380999/1‑140 (MQ=255)
                                                                                                                                  a‑a‑CTCCCCGCCAGTGTAGCGATTTCACGCTCAGCAACATGGCGCGGCACACCAGCTAATAGAACTTCTCCACGCATCTGCACAATGTTCCCGCGCTCGCCAAGTTGCAAGGTGTTAAACGATGCCACGGGCGAGACTTcc  <  1:329866/140‑1 (MQ=255)
                                                                                                                                  a‑a‑CTCCCCGCCAGTGTAGCGATTTCACGCTCAGCAACATGGCGCGGCACACCAGCTAATAGAACTTCTCCACGCATCTGCACAATGTTCCCGCGCTCGCCAAGTTGCAAGGTGTTAAACGATGCCACGGGCGAGACTTcc  >  1:1841242/1‑140 (MQ=255)
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GCACTGACGCGCTTTTCACCGACGACAAAAAAGCGTTCGGTGATATTTTCACTTTCGACTTCAAGCGAGACGGTATTACCCGGCCCCTGGTCGCGCGGCAGGTTATGAATATTCTGTTCATGCAGGGAAAA‑A‑CTCCCCGCCAGTGTAGCGATTTCACGCTCAGCAACATGGCGCGGCACACCAGCTAATAGAACTTCTCCACGCATCTGCACAATGTTCCCGCGCTCGCCAAGTTGCAAGGTGTTAAACGATGCCACGGGCGAGACTTCC  >  NC_000913/3556074‑3556343

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 24 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑