Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	233,152	C→A	P268P (CCG→CCT)	mltD ←	membrane‑bound lytic murein transglycosylase D

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	233,152	0	C	A	100.0%	274.0 / NA	77	P268P (CCG→CCT)	mltD	membrane‑bound lytic murein transglycosylase D
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base A (52/25);  total (52/25)

TGATCTGCATGCTTCTTTGGCACCATCACGTACTGCGGGCCACTTGCGCCCAGCGTGGAGCCTTTTACGCCAGCGTTGAATGTCTTCAGCTTGCTGACGGAAATCCCCGCCATATCTGCTACCTTCGCCATTTCAACCGGGCTGCTCAGGTGCACACGCGCCAGAGCACGGCTTTCATCGGTCGTTGGCAGACGTACGCCATAACGCTTGCTGTTTTTGAGAATATCACTCAATGCCAGCATTTTAGGCACGTACTGCTTCGTTTCCTGCG  >  NC_000913/233015‑233285                                                                                                                                          |                                                                                                                                      tGATCTGCATGCTTCTTTGGCACCATCACGTACTGCGGGCCACTTGCGCCCAGCGTGGAGCCTTTTACGCCAGCGTTGAATGTCTTCAGCTTGCTGACGGAAATCCCCGCCATATCTGCTACCTTCGCCATTTCAACAgg                                                                                                                                     >  1:485125/1‑140 (MQ=255)  gATCGGCTTGCTTTTTGGCCCCCATCCGGTGCGGCGGCCCTCTTGCGCCCGGGGTGGCCCTTTTTCCCCCGGCTTTGATTTTCTTCAGCTTGCTGACGAAAACCCCCGCCATATCTGCTACCTTCGCCATTTCAACAggg                                                                                                                                    <  2:1899187/140‑1 (MQ=255)          tGCTTCTTTGGCACCATCACGTACTGCGGGCCACTTGCGCCCAGCGTGGAGCCTTTTACGCCAGCGTTGAATGTCTTCAGCTTGCTGACGGAAATCCCCGCCATATCTGCTACCTTCGCCATTTCAACAGGGCTGCTCAg                                                                                                                            >  1:640970/1‑140 (MQ=255)          tGCTTCTTTGGCACCATCACGTACTGCGGGCCACTTGCGCCCAGCGTGGAGCCTTTTACGCCAGCGTTGAATGTCTTCAGCTTGCTGACGGAAATCCCCGCCATATCTGCTACCTTCGCCATTTCAACAGGGCTGCTCAg                                                                                                                            >  1:575128/1‑140 (MQ=255)          tGCTTCTTTGGCACCATCACGTACTGCGGGCCACTTGCGCCCAGCGTGGAGCCTTTTACGCCAGCGTTGAATGTCTTCAGCTTGCTGACGGAAATCCCCGCCATATCTGCTACCTTCGCCATTTCAACAGGGCTGCTCAg                                                                                                                            >  1:518733/1‑140 (MQ=255)          tGCTTCTTTGGCACCATCACGTACTGCGGGCCACTTGCGCCCAGCGTGGAGCCTTTTACGCCAGCGTTGAATGTCTTCAGCTTGCTGACGGAAATCCCCGCCATATCTGCTACCTTCGCCATTTCAACAAGGCTGCTCAg                                                                                                                            >  1:480522/1‑140 (MQ=255)             ttcttTGGCACCATCACGTACTGCGGGCCACTTGCGCCCAGCGTGGAGCCTTTTACGCCAGCGTTGAATGTCTTCAGCTTGCTGACGGAAATCCCCGCCATATCTGCTACCTTCGCCATTTCAACAGGGCTGCTCAGGTg                                                                                                                         >  1:266082/1‑140 (MQ=255)              tcttTGGCACCATCACGTACTGCGGGCCACTTGCGCCCAGCGTGGAGCCTTTTACGCCAGCGTTGAATGTCTTCAGCTTGCTGACGGAAATCCCCGCCATATCTGCTACCTTCGCCATTTCAACAGGGCTGCTCAGGTGc                                                                                                                        >  2:1127387/1‑140 (MQ=255)               cttTGGCACCATCACGTACTGCGGGCCACTTGCGCCCAGCGTGGAGCCTTTTACGCCAGCGTTGAATGTCTTCAGCTTGCTGACGGAAATCCCCGCCATATCTGCTACCTTCGCCATTTCAACAGGGCTGCTCAGGTGca                                                                                                                       >  2:757245/1‑140 (MQ=255)               cttTGGCACCATCACGTACTGCGGGCCACTTGCGCCCAGCGTGGAGCCTTTTACGCCAGCGTTGAATGTCTTCAGCTTGCTGACGGAAATCCCCGCCATATCTGCTACCTTCGCCATTTCAACAGGGCTGCTCAGGTGca                                                                                                                       >  1:1574374/1‑140 (MQ=255)                 ttgcccccATCACGTGCGGGGGGCCCCTTGCGCCCGGCGTGGAGCTTTTCCCGCCAGTTTTGATTTTCTCCAGTTTGCGGAGGAAAACCCCCGCCATATCTGCTACCTTCGCCATTTCAACAGGGCTGCTCAGGTGcaca                                                                                                                     <  2:1219817/134‑1 (MQ=255)                  tGGCACCATCACGTACTGCGGGCCACTTGCGCCCAGCGTGGAGCCTTTTACGCCAGCGTTGAATGTCTTCAGCTTGCTGACGGAAATCCCCGCCATATCTGCTACCTTCGCCATTTCAACAGGGCTGCTCAGGTGCacac                                                                                                                    >  1:1174728/1‑140 (MQ=255)                  tGGCACCATCACGTACTGCGGGCCACTTGCGCCCAGCGTGGAGCCTTTTACGCCAGCGTTGAATGTCTTCAGCTTGCTGACGGAAATCCCCGCCATATCTGCTACCTTCGCCATTTCAACAGGGCTGCTCAGGTGCacac                                                                                                                    >  1:1882188/1‑140 (MQ=255)                     cACCATCACGTACTGCGGGCCACTTGCGCCCAGCGTGGAGCCTTTTACGCCAGCGTTGAATGTCTTCAGCTTGCTGACGGAAATCCCCGCCATATCTGCTACCTTCGCCATTTCAACAGGGCTGCTCAGGTGCACAcgcg                                                                                                                 >  1:622491/1‑140 (MQ=255)                       ccATCACGTACTGCGGGCCACTTGCGCCCAGCGTGGAGCCTTTTACGCCAGCGTTGAATGTCTTCAGCTTGCTGACGGAAATCCCCGCCATATCTGCTACCTTCGCCATTTCAACAGGGCTGCTCAGGTGCACACGCGcc                                                                                                               >  1:233008/1‑140 (MQ=255)                          tCACGTACTGCGGGCCACTTGCGCCCAGCGTGGAGCCTTTTACGCCAGCGTTGAATGTCTTCAGCTTGCTGACGGAAATCCCCGCCATATCTGCTACCTTCGCCATTTCAACAGGGCTGCTCAGGTGCACACGCGCCaga                                                                                                            >  1:1828372/1‑140 (MQ=255)                               tACTGCGGGCCACTTGCGCCCAGCGTGGAGCCTTTTACGCCAGCGTTGAATGTCTTCAGCTTGCTGACGGAAATCCCCGCCATATCTGCTACCTTCGCCATTTCAACAGGGCTGCTCAGGTGCACACGCGCCAGAGCACg                                                                                                       >  1:970509/1‑140 (MQ=255)                                    cGGGCCACTTGCGCCCAGCGTGGAGCCTTTTACGCCAGCGTTGAATGTCTTCAGCTTGCTGACGGAAATCCCCGCCATATCTGCTACCTTCGCCATTTCAACAGGGCTGCTCAGGTGCACACGCGCCAGAGCACGGCttt                                                                                                  >  2:2373460/1‑140 (MQ=255)                                    cGGGCCACTTGCGCCCAGCGTGGAGCCTTTTACGCCAGCGTTGAATGTCTTCAGCTTGCTGACGGAAATCCCCGCCATATCTGCTACCTTCGCCATTTCAACAGGGCTGCTCAGGTGCACACGCGCCAGAGCACGGCttt                                                                                                  >  2:974892/1‑140 (MQ=255)                                    cGGGCCACTTGCGCCCAGCGTGGAGCCTTTTACGCCAGCGTTGAATGTCTTCAGCTTGCTGACGGAAATCCCCGCCATATCTGCTACCTTCGCCATTTCAACAGGGCTGCTCAGGTGCACACGCGCCAGAGCACGGCttt                                                                                                  >  1:2223329/1‑140 (MQ=255)                                    cGGGCCACTTGCGCCCAGCGTGGAGCCTTTTACGCCAGCGTTGAATGTCTTCAGCTTGCTGACGGAAATCCCCGCCATATCTGCTACCTTCGCCATTTCAACAGGGCTGCTCAGGTGCACACGCGCCAGAGCACGGCttt                                                                                                  >  2:496483/1‑140 (MQ=255)                                    cGGGCCACTTGCGCCCAGCGTGGAGCCTTTTACGCCAGCGTTGAATGTCTTCAGCTTGCTGACGGAAATCCCCGCCATATCTGCTACCTTCGCCATTTCAACAGGGCTGCTCAGGTGCACACGCGCCAGAGCACGGCttt                                                                                                  >  2:49833/1‑140 (MQ=255)                                      ggCCACTTGCGCCCAGCGTGGAGCCTTTTACGCCAGCGTTGAATGTCTTCAGCTTGCTGACGGAAATCCCCGCCATATCTGCTACCTTCGCCATTTCAACAGGGCTGCTCAGGTGCACACGCGCCAGAGCACGGCTTTCa                                                                                                >  1:909451/1‑140 (MQ=255)                                      ggCCACTTGCGCCCAGCGTGGAGCCTTTTACGCCAGCGTTGAATGTCTTCAGCTTGCTGACGGAAATCCCCGCCATATCTGCTACCTTCGCCATTTCAACAGGGCTGCTCAGGTGCACACGCGCCAGAGCACGGCTTTCa                                                                                                >  2:1131888/1‑140 (MQ=255)                                      ggCCACTTGCGCCCAGCGTGGAGCCTTTTACGCCAGCGTTGAATGTCTTCAGCTTGCTGACGGAAATCCCCGCCATATCTGCTACCTTCGCCATTTCAACAGGGCTGCTCAGGTGCACACGCGCCAGAGCACGGCTTTCa                                                                                                >  1:1471217/1‑140 (MQ=255)                                       gCCACTTGCGCCCAGCGTGGAGCCTTTTACGCCAGCGTTGAATGTCTTCAGCTTGCTGACGGAAATCCCCGCCATATCTGCTACCTTCGCCATTTCAACAGGGCTGCTCAGGTGCACACGCGCCAGAGCACGGCTTTCAt                                                                                               >  1:55269/1‑140 (MQ=255)                                       ccctctggcgcCCAGCGTGGACCCTTTTGCCCCGGTTTTGAATGTCTTCAGGTTGCGGGCGGAACCCCCCGCCATATCTGCTACCTTCGCCATTTCAACAGGGCTGCTCAGGTGCACACGCGCCAGAGCACGGCTTTCAt                                                                                               <  2:754983/133‑1 (MQ=255)                                        ccACTTGCGCCCAGCGTGGAGCCTTTTACGCCAGCGTTGAATGTCTTCAGCTTGCTGACGGAAATCCCCGCCATATCTGCTACCTTCGCCATTTCAACAGGGCTGCTCAGGTGCACACGCGCCAGAGCACGGCTTTCATc                                                                                              >  2:651092/1‑140 (MQ=255)                                            ttGCGCCCAGCGTGGAGCCTTTTACGCCAGCGTTGAATGTCTTCAGCTTGCTGACGGAAATCCCCGCCATATCTGCTACCTTCGCCATTTCAACAGGGCTGCTCAGGTGCACACGCGCCAGAGCACGGCTTTCATCGGTc                                                                                          >  1:1864789/1‑140 (MQ=255)                                            ttGCGCCCAGCGTGGAGCCTTTTACGCCAGCGTTGAATGTCTTCAGCTTGCTGACGGAAATCCCCGCCATATCTGCTACCTTCGCCATTTCAACAGGGCTGCTCAGGTGCACACGCGCCAGAGCACGGCTTTCATCGGTc                                                                                          >  1:1891507/1‑140 (MQ=255)                                            ttGCCCCCGGGGTGGACCTTTTTACCCCAGTTTTGTATTTTTTTCGCTCGCGGACGAAAACCCCCGCCATATCTGCTACCTTCGCCATTTCAACAGGGCTGCTCAGGTGCACACGCGCCAGAGCACGGCTTTCATCGGTc                                                                                          <  2:1828372/140‑1 (MQ=255)                                                      cGTGGAGCCTTTTACGCCAGCGTTGAATGTCTTCAGCTTGCTGACGGAAATCCCCGCCATATCTGCTACCTTCGCCATTTCAACAGGGCTGCTCAGGTGCACACGCGCCAGAGCACGGCTTTCATCGGTCGTTGGCAGAc                                                                                >  1:2129875/1‑140 (MQ=255)                                                         agactttttcACCCCGGCTTTGATTTTCTGCAGTTGGCTGACGAAACCCCCCGCCATATCTGCTACCTTCGCCATTTCAACAGGGCTGCTCAGGTGCACACGCGCCAGAGCACGGCTTTCATCGGTCGTTGGCAGACGTa                                                                             <  2:2022694/134‑1 (MQ=255)                                                               ttttCCCCCAGTTTTGATTTTCTCCAGCTTGTGAACGAAAACCCCCGCCATATCTGCTACCTTCGCCATTTCAACAGGGCTGCTCAGGTGCACACGCGCCAGAGCACGGCTTTCATCGGTCGTTGGCAGACGTACGCCAt                                                                       <  2:1882188/140‑1 (MQ=255)                                                                tttACGCCAGCGTTGAATGTCTTCAGCTTGCTGACGGAAATCCCCGCCATATCTGCTACCTTCGCCATTTCAACAGGGCTGCTCAGGTGCACACGCGCCAGAGCACGGCTTTCATCGGTCGTTGGCAGACGTACGCCATa                                                                      >  1:1202860/1‑140 (MQ=255)                                                                          ggTTGAATGTCTTCAGCTTGCTGAGGGAACTCCCCGCCATATCTGCTACCTTCGCCATTTCAACAGGGCTGCTCAGGTGCACACGCGCCAGAGCACGGCTTTCATCGGTCGTTGGCAGACGTACGCCATAACGCTTGCTg                                                            <  1:346368/139‑1 (MQ=255)                                                                          cGTTGAATGTCTTCAGCTTGCTGACGGAAATCCCCGCCATATCTGCTACCTTCGCCATTTCAACAGGGCTGCTCAGGTGCACACGCGCCAGAGCACGGCTTTCATCGGTCGTTGGCAGACGTACGCCATAACGCTTGCTg                                                            >  2:222870/1‑140 (MQ=255)                                                                          cGTTGAATGTCTTCAGCTTGCTGACGGAAATCCCCGCCATATCTGCTACCTTCGCCATTTCAACAGGGCTGCTCAGGTGCACACGCGCCAGAGCACGGCTTTCATCGGTCGTTGGCAGACGTACGCCATAACGCTTGCTg                                                            >  1:518302/1‑140 (MQ=255)                                                                          cGTTGAATGTCTTCAGCTTGCTGACGGAAATCCCCGCCATATCTGCTACCTTCGCCATTTCAACAGGGCTGCTCAGGTGCACACGCGCCAGAGCACGGCTTTCATCGGTCGTTGGCAGACGTACGCCATAACGCTTGCTg                                                            >  1:1809573/1‑140 (MQ=255)                                                                          cGTTGAATGTCTTCAGCTTGCTGACGGAAATCCCCGCCATATCTGCTACCTTCGCCATTTCAACAGGGCTGCTCAGGTGCACACGCGCCAGAGCACGGCTTTCATCGGTCGTTGGCAGACGTACGCCATAACGCCTgtct                                                            >  1:2022694/1‑137 (MQ=255)                                                                                 tGTCTGCGGCTTGCTGACGAAACCCCCCGCCATATCTGCTACCTTCGCCATTTCAACAGGGCTGCTCAGGTGCACACGCGCCAGAGCACGGCTTTCATCGGTCGTTGGCAGACGTACGCCATAACGCTTGCTGTTTTTga                                                     <  1:2024670/140‑1 (MQ=255)                                                                                       cAGCTGGCGGACGAAAATCCCCGCCATATCTGCTACCTTCGCCATTTCAACAGGGCTGCTCAGGTGCACACGCGCCAGAGCACGGCTTTCATCGGTCGTTGGCAGACATACGCCATAACGCTTGCTGTTTTTGAGAatat                                               <  2:2372609/140‑1 (MQ=255)                                                                                         gCTTGCTGACGGAAATCCCCGCCATATCTGCTACCTTCGCCATTTCAACAGGGCTGCTCAGGTGCACACGCGCCAGAGCACGGCTTTCATCGGTCGTTGGCAGACGTACGCCATAACGCTTGCTGTTTTTGAGAATATCa                                             >  2:618546/1‑140 (MQ=255)                                                                                             gCTGACGGAAATCCCCGCCATATCTGCTACCTTCGCCATTTCAACAGGGCTGCTCAGGTGCACACGCGCCAGAGCACGGCTTTCATCGGTCGTTGGCAGACGTACGCCATAACGCTTGCTGTTTTTGAGAATATCACTCa                                         >  2:595918/1‑140 (MQ=255)                                                                                             gCTGACGGAAATCCCCGCCATATCTGCTACCTTCGCCATTTCAACAGGGCTGCTCAGGTGCACACGCGCCAGAGCACGGCTTTCATCGGTCGTTGGCAGACGTACGCCATAACGCTTGCTGTTTTTGAGAATATCACTCa                                         >  1:1069651/1‑140 (MQ=255)                                                                                              cTGACGGAAATCCCCGCCATATCTGCTACCTTCGCCATTTCAACAGGGCTGCTCAGGTGCACACGCGCCAGAGCACGGCTTTCATCGGTCGTTGGCAGACGTACGCCATAACGCTTGCTGTTTTTGAGAATATCACTCaa                                        >  1:1691830/1‑140 (MQ=255)                                                                                                    gAAATCCCCGCCATATCTGCTACCTTCGCCATTTCAACAGGGCTGCTCAGGTGCACACGCGCCAGAGCACGGCTTTCATCGGTCGTTGGCAGACGTACGCCATAACGCTTGCTGTTTTTGAGAATATCACTCAATGccct                                  >  1:1259784/1‑138 (MQ=255)                                                                                                    gAAATCCCCGCCATATCTGCTACCTTCGCCATTTCAACAGGGCTGCTCAGGTGCACACGCGCCAGAGCACGGCTTTCATCGGTCGTTGGCAGACGTACGCCATAACGCTTGCTGTTTTTGAGAATATCACTCAATGCCAg                                  <  1:595918/140‑1 (MQ=255)                                                                                                       aTCCCCGCCATATCTGCTACCTTCGCCATTTCAACAGGGCTGCTCAGGTGCACACGCGCCAGAGCACGGCTTTCATCGGTCGTTGGCAGACGTACGCCATAACGCTTGCTGTTTTTGAGAATATCACTCAATGCCAGCAt                               >  2:642036/1‑140 (MQ=255)                                                                                                         ccccGCCATATCTGCTACCTTCGCCATTTCAACAGGGCTGCTCAGGTGCACACGCGCCAGAGCACGGCTTTCATCGGTCGTTGGCAGACGTACGCCATAACGCTTGCTGTTTTTGAGAATATCACTCAATGCCAGCAttt                             <  2:970509/140‑1 (MQ=255)                                                                                                         ccccGCCATATCTGCTACCTTCGCCATTTCAACAGGGCTGCTCAGGTGCACACGCGCCAGAGCACGGCTTTCATCGGTCGTTGGCAGACGTACGCCATAACGCTTGCTGTTTTTGAGAATATCACTCAATGCCAGCAttt                             <  2:1177924/140‑1 (MQ=255)                                                                                                         ccccGCCATATCTGCTACCTTCGCCATTTCAACAGGGCTGCTCAGGTGCACACGCGCCAGAGCACGGCTTTCATCGGTCGTTGGCAGACGTACGCCATAACGCTTGCTGTTTTTGAGAATATCACTCAATGCCAGCAttt                             <  1:1127387/140‑1 (MQ=255)                                                                                                         ccccGCCATATCTGCTACCTTCGCCATTTCAACAGGGCTGCTCAGGTGCACACGCGCCAGAGCACGGCTGTCATCGGTCGTTGGCAGACGTACGCCATAACGCTTGCTGTTTTTGAGAATATCACTCAATGCCAGCAttt                             <  2:2129875/140‑1 (MQ=255)                                                                                                            cGCCATATCTGCTACCTTCGCCATTTCAACAGGGCTGCTCAGGTGCACACGCGCCAGAGCACGGCTTTCATCGGTCGTTGGCAGACGTACGCCATAACGCTTGCTGTTTTTGAGAATATCACTCAATGCCAGCATTTTAg                          <  2:1210267/140‑1 (MQ=255)                                                                                                            cGCCATATCTGCTACCTTCGCCATTTCAACAGGGCTGCTCAGGTGCACACGCGCCAGAGCACGGCTTTCATCGGTCGTTGGCAGACGTACGCCATAACGCTTGCTGTTTTTGAGAATATCACTCAATGCCAGCATTTTAg                          >  1:1299600/1‑140 (MQ=255)                                                                                                            cGCCATATCTGCTACCTTCGCCATTTCAACAGGGCTGCTCAGGTGCACACGCGCCAGAGCACGGCTTTCATCGGTCGTTGGCAGACGTACGCCATAACGCTTGCTGTTTTTGAGAATATCACTCAATGCCAGCATTTTAg                          <  1:618546/140‑1 (MQ=255)                                                                                                             gCCATATCTGCTACCTTCGCCATTTCAACAGGGCTGCTCAGGTGCACACGCGCCAGAGCACGGCTTTCATCGGTCGTTGGCAGACGTACGCCATAACGCTTGCTGTTTTTGAGAATATCACTCAATGCCAGCATTTTAgg                         <  1:49833/140‑1 (MQ=255)                                                                                                              ccATATCTGCTACCTTCGCCATTTCAACAGGGCTGCTCAGGTGCACACGCGCCAGAGCACGGCTTTCATCGGTCGTTGGCAGACGTACGCCATAACGCTTGCTGTTTTTGAGAATATCACTCAATGCCAGCATTTTAGGc                        <  2:55269/140‑1 (MQ=255)                                                                                                              ccATATCTGCTACCTTCGCCATTTCAACAGGGCTGCTCAGGTGCACACGCGCCAGAGCACGGCTTTCATCGGTCGTTGGCAGACGTACGCCATAACGCTTGCTGTTTTTGAGAATATCACTCAATGCCAGCATTTTAGGc                        <  1:876033/140‑1 (MQ=255)                                                                                                                 tatCTGCTACCTTCGCCATTTCAACAGGGCTGCTCAGGTGCACACGCGCCAGAGCACGGCTTTCATCGGTCGTTGGCAGACGTACGCCATAACGCTTGCTGTTTTTGAGAATATCACTCAATGCCAGCATTTTAGGCACg                     <  2:1202860/140‑1 (MQ=255)                                                                                                                 tatCTGCTACCTTCGCCATTTCAACAGGGCTGCTCAGGTGCACACGCGCCAGAGCACGGCTTTCATCGGTCGTTGGCAGACGTACGCCATAACGCTTGCTGTTTTTGAGAATATCACTCAATGCCAGCATTTTAGGCACg                     >  2:1714685/1‑140 (MQ=255)                                                                                                                   tCTGCTACCTTCGCCATTTCAACAGGGCTGCTCAGGTGCACACGCGCCAGAGCACGGCTTTCATCGGTCGTTGGCAGACGTACGCCATAACGCTTGCTGTTTTTGAGAATATCACTCAATGCCAGCATTTTAGGCACGTa                   <  1:642036/140‑1 (MQ=255)                                                                                                                     tGCTACCTTCGCCATTTCAACAGGGCTGCTCAGGTGCACACGCGCCAGAGCACGGCTTTCATCGGTCGTTGGCAGACGTACGCCATAACGCTTGCTGTTTTTGAGAATATCACTCAATGCCAGCATTTTAGGCACGTACt                 <  1:1150747/140‑1 (MQ=255)                                                                                                                        tACCTTCGCCATTTCAACAGGGCTGCTCAGGTGCACACGCGCCAGAGCACGGCTTTCATCGGTCGTTGGCAGACGTACGCCATAACGCTTGCTGTTTTTGAGAATATCACTCAATGCCAGCATTTTAGGCACGTACTGCt              >  2:1081434/1‑140 (MQ=255)                                                                                                                           cTTCGCCATTTCAACAGGGCTGCTCAGGTGCACACGCGCCAGAGCACGGCTTTCATCGGTCGTTGGCAGACGTACGCCATAACGCTTGCTGTTTTTGAGAATATCACTCAATGCCAGCATTTTAGGCACGTACTGCTTCg           >  2:1862079/1‑140 (MQ=255)                                                                                                                           cTTCGCCATTTCAACAGGGCTGCTCAGGTGCACACGCGCCAGAGCACGGCTTTCATCGGTCGTTGGCAGACGTACGCCATAACGCTTGCTGTTTTTGAGAATATCACTCAATGCCAGCATTTTAGGCACGTACTGCTTCg           <  1:792098/140‑1 (MQ=255)                                                                                                                            ttCGCCATTTCAACAGGGCTGCTCAGGTGCACACGCGCCAGAGCACGGCTTTCATCGGTCGTTGGCAGACGTACGCCATAACGCTTGCTGTTTTTGAGAATATCACTCAATGCCAGCATTTTAGGCACGTACTGCTTCGt          >  2:622723/1‑140 (MQ=255)                                                                                                                             tCGCCATTTCAACAGGGCTGCTCAGGTGCACACGCGCCAGAGCACGGCTTTCATCGGTCGTTGGCAGACGTACGCCATAACGCTTGCTGTTTTTGAGAATATCACTCAATGCCAGCATTTTAGGCACGTACTGCTTCGtt         >  1:743010/1‑140 (MQ=255)                                                                                                                             tCGCCATTTCAACAGGGCTGCTCAGGTGCACACGCGCCAGAGCACGGCTTTCATCGGCCGTTGGCAGACGTACGCCATAACGCTTGCTGTTTTTGAGAATATCACTCAATGCCAGCATTTTAGGCACGTACTGCTTCGtt         >  1:2109617/1‑140 (MQ=255)                                                                                                                              cGCCATTTCAACAGGGCTGCTCAGGTGCACACGCGCCAGAGCACGGCTTTCATCGGTCGTTGGCAGACGTACGCCATAACGCTTGCTGTTTTTGAGAATATCACTCAATGCCAGCATTTTAGGCACGTACTGCTTCGttt        >  1:1386345/1‑140 (MQ=255)                                                                                                                              cGCCATTTCAACAGGGCTGCTCAGGTGCACACGCGCCAGAGCACGGCTTTCATCGGTCGTTGGCAGACGTACGCCATAACGCTTGCTGTTTTTGAGAATATCACTCAATGCCAGCATTTTAGGCACGTACTGCTTCGttt        >  1:764991/1‑140 (MQ=255)                                                                                                                              cGCCATTTCAACAGGGCTGCTCAGGTGCACACGCGCCAGAGCACGGCTTTCATCGGTCGTTGGCAGACGTACGCCATAACGCTTGCTGTTTTTGAGAATATCACTCAATGCCAGCATTTTAGGCACGTACTGCTTCGttt        >  2:872897/1‑140 (MQ=255)                                                                                                                              cGCCATTTCAACAGGGCTGCTCAGGTGCACACGCGCCAGAGCACGGCTTTCATCGGTCGTTGGCAGACGTACGCCATAACGCTTGCTGTTTTTGAGAATATCACTCAATGCCAGCATTTTAGGCACGTACTGCTTCGttt        >  1:1025682/1‑140 (MQ=255)                                                                                                                               gctaTTTCAACAGGGCTGCTCAGGTGCACACGCGCCAGAGCACGGCTTTCATCGGTCGTTGGCAGACGTACGCCATAACGCTTGCTGTTTTTGAGAATATCACTCAATGCCAGCATTTTAGGCACGTACTGCTTCGTTTc       >  2:1602196/4‑140 (MQ=255)                                                                                                                                 cATTTCAACAGGGCTGCTCAGGTGCACACGCGCCAGAGCACGGCTTTCATCGGTCGTTGGCAGACGTACGCCATAACGCTTGCTGTTTTTGAGAATATCACTCAATGCCAGCATTTTAGGCACGTACTGCTTCGTTTCCt     >  1:900039/1‑140 (MQ=255)                                                                                                                                   tttCAACAGGGCTGCTCAGGTGCACACGCGCCAGAGCACGGCTTTCATCGGTCGTTGGCAGACGTACGCCATAACGCTTGCTGTTTTTGAGAATATCACTCAATGCCAGCATTTTAGGCACGTACTGCTTCGTTTCCTGc   <  1:651092/140‑1 (MQ=255)                                                                                                                                   tttCAACAGGGCTGCTCAGGTGCACACGCGCCAGAGCACGGCTTTCATCGGTCGTTGGCAGACGTACGCCATAACGCTTGCTGTTTTTGAGAATATCACTCAATGCCAGCATTTTAGGCACGTACTGCTTCGTTTCCTGc   <  1:757245/140‑1 (MQ=255)                                                                                                                                    ttCAACAGGGCTGCTCAGGTGCACACGCGCCAGAGCACGGCTTTCATCGGTCGTTGGCAGACGTACGCCATAACGCTTGCTGTTTTTGAGAATATCACTCAATGCCAGCATTTTAGGCACGTACTGCTTCGTTTCCTGCg  >  2:229086/1‑140 (MQ=255)                                                                                                                                          |                                                                                                                                      TGATCTGCATGCTTCTTTGGCACCATCACGTACTGCGGGCCACTTGCGCCCAGCGTGGAGCCTTTTACGCCAGCGTTGAATGTCTTCAGCTTGCTGACGGAAATCCCCGCCATATCTGCTACCTTCGCCATTTCAACCGGGCTGCTCAGGTGCACACGCGCCAGAGCACGGCTTTCATCGGTCGTTGGCAGACGTACGCCATAACGCTTGCTGTTTTTGAGAATATCACTCAATGCCAGCATTTTAGGCACGTACTGCTTCGTTTCCTGCG  >  NC_000913/233015‑233285

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 24 ≤ ATCG/ATCG < 30 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑