BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	1,333,590	C→A	A848E (GCG→GAG)	topA →	DNA topoisomerase 1

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,333,590	0	C	A	100.0%	506.1 / NA	141	A848E (GCG→GAG)	topA	DNA topoisomerase 1
Reads supporting (aligned to +/- strand): ref base C (0/0); new base A (68/73); total (68/73)

Only 100 of 145 total aligned reads displayed.

CTTCCGCGACCGTCTGCCGGAAAAACTGCGTTATCTGGCCGATGCGCCACAGCAGGATCCGGAAGGTAATAAGACCATGGTTCGCTTTAGCCGTAAAACCAAACAGCAATATGTCTCTTCGGAAAAAGACGGAAAGGCGACTGGCTGGTCAGCATTTTATGTTGATGGCAAATGGGTTGAAGGAAAAAAATAACCTTTAATTCTGTCAGGTTTTTATAAACAAAGGGTCGCGAAAGCGGCCCTTTTTTATTGCATATTATTTTTTCTTCACACC  >  NC_000913/1333453‑1333726

cTTCCGCGACCGTCTGCCGGAAAAACTGCGTTATCTGGCCGATGCGCCACAGCAGGACCCGGAAGGTAATAAGACCATGGTTCGCTTTAGCCGTAAAACCAAACAGCAATATGTCTCTTCGGAAAAAGACGGAAAGgaga                                                                                                                                        <  1:1076831/140‑1 (MQ=255)

  tCCGCGACCGTCTGCCGGAAAAACTGCGTTATCTGGCCGATGCGCCACAGCAGGATCCGGAAGGTAATAAGACCATGGTTCGCTTTAGCCGTAAAACCAAACAGCAATATGTCTCTTCGGAAAAAGACGGAAAGGAGAct                                                                                                                                      >  2:149185/1‑140 (MQ=255)

    cgcgACCGTCTGCCGGAAAAACTGCGTTATCTGGCCGATGCGCCACAGCAGGATCCGGAAGGTAATAAGACCATGGTTCGCTTTAGCCGTAAAACCAAACAGCAATATGTCTCTTCGGAAAAAGACGGAAAGGAGActgg                                                                                                                                    <  1:294239/140‑1 (MQ=255)

     gcgACCGTCTGCCGGAAAAACTGCGTTATCTGGCCGATGCGCCACAGCAGGATCCGGAAGGTAATAAGACCATGGTTCGCTTTAGCCGTAAAACCAAACAGCAATATGTCTCTTCGGAAAAAGACGGAAAGGAGActggc                                                                                                                                   <  1:2090598/140‑1 (MQ=255)

      cgACCGTCTGCCGGAAAAACTGCGTTATCTGGCCGATGCGCCACAGCAGGATCCGGAAGGTAATAAGACCATGGTTCGCTTTAGCCGTAAAACCAAACAGCAATATGTCTCTTCGGAAAAAGACGGAAAGGAGActggct                                                                                                                                  >  1:879797/1‑140 (MQ=255)

        aCCGTCTGCCGGAAAAACTGCGTTATCTGGCCGATGCGCCACAGCAGGATCCGGAAGGTAATAAGACCATGGTTCGCTTTAGCCGTAAAACCAAACAGCAATATGTCTCTTCGGAAAAAGACGGAAAGGAGActggctgg                                                                                                                                <  2:2192552/140‑1 (MQ=255)

         ccGTCTGCCGGAAAAACTGCGTTATCTGGCCGATGCGCCACAGCAGGATCCGGAAGGTAATAAGACCATGGTTCGCTTTAGCCGTAAAACCAAACAGCAATATGTCTCTTCGGAAAAAGACGGAAAGGAGACTGGCTGGt                                                                                                                               <  2:391677/140‑1 (MQ=255)

         ccGTCTGCCGGAAAAACTGCGTTATCTGGCCGATGCGCCACAGCAGGATCCGGAAGGTAATAAGACCATGGTTCGCTTTAGCCGTAAAACCAAACAGCAATATGTCTCTTCGGAAAAAGACGGAAAGGAGACTGGCTGGt                                                                                                                               <  1:880681/140‑1 (MQ=255)

          cGTCTGCCGGAAAAACTGCGTTATCTGGCCGATGCGCCACAGCAGGATCCGGAAGGTAATAAGACCATGGTTCGCTTTAGCCGTAAAACCAAACAGCAATATGTCTCTTCGGAAAAAGACGGAAAGGAGACTGGCTGGTc                                                                                                                              <  1:1640044/140‑1 (MQ=255)

           gTCTGCCGGAAAAACTGCGTTATCTGGCCGATGCGCCACAGCAGGATCCGGAAGGTAATAAGACCATGGTTCGCTTTAGCCGTAAAACCAAACAGCAATATGTCTCTTCGGAAAAAGACGGAAAGGAGACTGGCTGGTCa                                                                                                                             <  2:1112731/140‑1 (MQ=255)

           gTCTGCCGGAAAAACTGCGTTATCTGGCCGATGCGCCACAGCAGGATCCGGAAGGTAATAAGACCATGGTTCGCTTTAGCCGTAAAACCAAACAGCAATATGTCTCTTCGGAAAAAGACGGAAAGGAGACTGGCTGGTCa                                                                                                                             <  1:1431257/140‑1 (MQ=255)

           gTCTGCCGGAAAAACTGCGTTATCTGGCCGATGCGCCACAGCAGGATCCGGAAGGTAATAAGACCATGGTTCGCTTTAGCCGTAAAACCAAACAGCAATATGTCTCTTCGGAAAAAGACGGAAAGGAGACTGGCTGGTCa                                                                                                                             <  1:387832/140‑1 (MQ=255)

           gTCTGCCGGAAAAACTGCGTTATCTGGCCGATGCGCCACAGCAGGATCCGGAAGGTAATAAGACCATGGTTCGCTTTAGCCGTAAAACCAAACAGCAATATGTCTCTTCGGAAAAAGACGGAAAGGAGACTGGCTGGTCa                                                                                                                             <  1:2054584/140‑1 (MQ=255)

            tCTGCCGGAAAAACTGCGTTATCTGGCCGATGCGCCACAGCAGGATCCGGAAGGTAATAAGACCATGGTTCGCTTTAGCCGTAAAACCAAACAGCAATATGTCTCTTCGGAAAAAGACGGAAAGGAGACTGGCTGGTCAg                                                                                                                            >  2:1449123/1‑140 (MQ=255)

                   gAAAAACTGCGTTATCTGGCCGATGCGCCACAGCAGGATCCGGAAGGTAATAAGACCATGGTTCGCTTTAGCCGTAAAACCAAACAGCAATATGTCTCTTCGGAAAAAGACGGAAAGGAGACTGGCTGGTCa                                                                                                                             <  1:1909126/132‑1 (MQ=255)

                       aaCTGCGTTATCTGGCCGATGCGCCACAGCAGGATCCGGAAGGTAATAAGACCATGGTTCGCTTTAGCCGTAAAACCAAACAGCAATATGTCTCTTCGGAAAAAGACGGAAAGGAGACTGGCTGGTCAGCATTTTATGtt                                                                                                                 <  1:1542417/140‑1 (MQ=255)

                        aCTGCGTTATCTGGCCGATGCGCCACAGCAGGATCCGGAAGGTAATAAGACCATGGTTCGCTTTAGCCGTAAAACCAAACAGCAATATGTCTCTTCGGAAAAAGACGGAAAGGAGACTGGCTGGTCAGCATTTTATGTTg                                                                                                                <  1:1501322/140‑1 (MQ=255)

                                 tCTGGCCGATGCGCCACAGCAGGATCCGGAAGGTAATAAGACCATGGTTCGCTTTAGCCGTAAAACCAAACAGCAATATGTCTCTTCGGAAAAAGACGGAAAGGAGACTGGCTGGTCAGCATTTTATGTTGAt                                                                                                              >  2:1099561/1‑133 (MQ=255)

                                 tCTGGCCGATGCGCCACAGCAGGATCCGGAAGGTAATAAGACCATGGTTCGCTTTAGCCGTAAAACCAAACAGCAATATGTCTCTTCGGAAAAAGACGGAAAGGAGACTGGCTGGTCAGCATTTTATGTTGAt                                                                                                              <  1:1099561/133‑1 (MQ=255)

                                     gCCGATGCGCCACAGCAGGATCCGGAAGGTAATAAGACCATGGTTCGCTTTAGCCGTAAAACCAAACAGCAATATGTCTCTTCGGAAAAAGACGGAAAGGAGACTGGCTGGTCAGCATTTTATGTTGATGGCAAATGGGt                                                                                                   <  1:79479/140‑1 (MQ=255)

                                          tGCGCCACAGCAGGATCCGGAAGGTAATAAGACCATGGTTCGCTTTAGCCGTAAAACCAAACAGCAATATGTCTCTTCGGAAAAAGACGGAAAGGAGACTGGCTGGTCAGCATTTTATGTTGATGGCAAATGGGTTGAAg                                                                                              >  1:2081991/1‑140 (MQ=255)

                                           gcgcCACAGCAGGATCCGGAAGGTAATAAGACCATGGTTCGCTTTAGCCGTAAAACCAAACAGCAATATGTCTCTTCGGAAAAAGACGGAAAGGAGACTGGCTGGTCAGCATTTTATGTTGATGGCAAATGGGTTGAAgg                                                                                             >  1:142879/1‑140 (MQ=255)

                                           gcgcCACAGCAGGATCCGGAAGGTAATAAGACCATGGTTCGCTTTAGCCGTAAAACCAAACAGCAATATGTCTCTTCGGAAAAAGACGGAAAGGAGACTGGCTGGTCAGCATTTTATGTTGATGGCAAATGGGTTGAAgg                                                                                             >  1:1507801/1‑140 (MQ=255)

                                            cgcCACAGCAGGATCCGGAAGGTAATAAGACCATGGTTCGCTTTAGCCGTAAAACCAAACAGCAATATGTCTCTTCGGAAAAAGACGGAAAGGAGACTGGCTGGTCAGCATTTTATGTTGATGGCAAATGGGTTGAAGGa                                                                                            <  1:569178/140‑1 (MQ=255)

                                                      ggATCCGGAAGGTAATAAGACCATGGTTCGCTTTAGCCGTAAAACCAAACAGCAATATGTCTCTTCGGAAAAAGACGGAAAGGAGACTGGCTGGTCAGCATTTTATGTTGATGGCAAATGGGTTGAAGGAAAAAAATAAc                                                                                  >  1:1616870/1‑140 (MQ=255)

                                                         tCCGGAAGGTAATAAGACCATGGTTCGCTTTAGCCGTAAAACCAAACAGCAATATGTCTCTTCGGAAAAAGACGGAAAGGAGACTGGCTGGTCAGCATTTTATGTTGATGGCAAATGGGTTGAAGGAAAAAAATAACCtt                                                                               <  2:795088/140‑1 (MQ=255)

                                                         tCCGGAAGGTAATAAGACCATGGTTCGCTTTAGCCGTAAAACCAAACAGCAATATGTCTCTTCGGAAAAAGACGGAAAGGAGACTGGCTGGTCAGCATTTTATGTTGATGGCAAATGGGTTGAAGGAAAAAAATAACCtt                                                                               >  1:795088/1‑140 (MQ=255)

                                                           cGGAAGGTAATAAGACCATGGTTCGCTTTAGCCGTAAAACCAAACAGCAATATGTCTCTTCGGAAAAAGACGGAAAGGAGACTGGCTGGTCAGCATTTTATGTTGATGGCAAATGGGTTGAAGGAAAAAAATa                                                                                    >  1:777349/1‑133 (MQ=255)

                                                           cGGAAGGTAATAAGACCATGGTTCGCTTTAGCCGTAAAACCAAACAGCAATATGTCTCTTCGGAAAAAGACGGAAAGGAGACTGGCTGGTCAGCATTTTATGTTGATGGCAAATGGGTTGAAGGAAAAAAATa                                                                                    <  2:777349/133‑1 (MQ=255)

                                                              aaGGTAATAAGACCATGGTTCGCTTTAGCCGTAAAACCAAACAGCAATATGTCTCTTCGGAAAAAGACGGAAAGGAGACTGGCTGGTCAGCATTTTATGTTGATGGCAAATGGGTTGAAGGAAAAAAATAACCTTTAAtt                                                                          <  2:1122726/140‑1 (MQ=255)

                                                                 gTAATAAGACCATGGTTCGCTTTAGCCGTAAAACCAAACAGCAATATGTCTCTTCGGAAAAAGACGGAAAGGAGACTGGCTGGTCAGCATTTTATGTTGATGGCAAATGGGTTGAAGGAAAAAAATAACCTTTaa                                                                            <  2:2143142/135‑1 (MQ=255)

                                                                 gTAATAAGACCATGGTTCGCTTTAGCCGTAAAACCAAACAGCAATATGTCTCTTCGGAAAAAGACGGAAAGGAGACTGGCTGGTCAGCATTTTATGTTGATGGCAAATGGGTTGAAGGAAAAAAATAACCTTTaa                                                                            >  1:2143142/1‑135 (MQ=255)

                                                                  taataaGACCATGGTTCGCTTTAGCCGTAAAACCAAACAGCAATATGTCTCTTCGGAAAAAGACGGAAAGGAGACTGGCTGGTCAGCATTTTATGTTGATGGCAAATGGGTTGAAGGAAAAAAATAACCTTTAATTCTGt                                                                      >  2:1253641/1‑140 (MQ=255)

                                                                   aataaGACCATGGTTCGCTTTAGCCGTAAAACCAAACAGCAATATGTCTCTTCGGAAAAAGACGGAAAGGAGACTGGCTGGTCAGCATTTTATGTTGATGGCAAATGGGTTGAAGGAAAAAAATAACCttt                                                                              >  2:1801074/1‑131 (MQ=255)

                                                                   aataaGACCATGGTTCGCTTTAGCCGTAAAACCAAACAGCAATATGTCTCTTCGGAAAAAGACGGAAAGGAGACTGGCTGGTCAGCATTTTATGTTGATGGCAAATGGGTTGAAGGAAAAAAATAACCttt                                                                              <  1:1801074/131‑1 (MQ=255)

                                                                      aaGACCATGGTTCGCTTTAGCCGTAAAACCAAACAGCAATATGTCTCTTCGGAAAAAGACGGAAAGGAGACTGGCTGGTCAGCATTTTATGTTGATGGCAAATGGGTTGAAGGAAAAAAATAACCTTTAATTCTGTCAgg                                                                  <  2:1045810/140‑1 (MQ=255)

                                                                      aaGACCATGGTTCGCTTTAGCCGTAAAACCAAACAGCAATATGTCTCTTCGGAAAAAGACGGAAAGGAGACTGGCTGGTCAGCATTTTATGTTGATGGCAAATGGGTTGAAGGAAAAAAATAACCTTTAATTCTGTCAgg                                                                  <  2:2152036/140‑1 (MQ=255)

                                                                      aaGACCATGGTTCGCTTTAGCCGTAAAACCAAACAGCAATATGTCTCTTCGGAAAAAGACGGAAAGGAGACTGGCTGGTCAGCATTTTATGTTGATGGCAAATGGGTTGAAGGAAAAAAATAACCTTTAATTCTGTCAgg                                                                  <  1:1252266/140‑1 (MQ=255)

                                                                      aaGACCATGGTTCGCTTTAGCCGTAAAACCAAACAGCAATATGTCTCTTCGGAAAAAGACGGAAAGGAGACTGGCTGGTCAGCATTTTATGTTGATGGCAAATGGGTTGAAGGAAAAAAATAACCTTTAATTCTGTCAgg                                                                  <  2:350249/140‑1 (MQ=255)

                                                                          ccATGGTTCGCTTTAGCCGTAAAACCAAACAGCAATATGTCTCTTCGGAAAAAGACGGAAAGGAGACTGGCTGGTCAGCATTTTATGTTGATGGCAAATGGGTTGAAGGAAAAAAATAACCTTTAATTCTGTCAGGtttt                                                              <  1:492763/140‑1 (MQ=255)

                                                                          ccATGGTTCGCTTTAGCCGTAAAACCAAACAGCAATATGTCTCTTCGGAAAAAGACGGAAAGGAGACTGGCTGGTCAGCATTTTATGTTGATGGCAAATGGGTTGAAGGAAAAAAATAACCTTTAATTCTGTCAGGtttt                                                              >  1:1545676/1‑140 (MQ=255)

                                                                           cATGGTTCGCTTTAGCCGTAAAACCAAACAGCAATATGTCTCTTCGGAAAAAGACGGAAAGGAGACTGGCTGGTCAGCATTTTATGTTGATGGCAAATGGGTTGAAGGAAAAAAATAACCTTTAATTCTGTCAGGttttt                                                             >  2:541317/1‑140 (MQ=255)

                                                                           cATGGTTCGCTTTAGCCGTAAAACCAAACAGCAATATGTCTCTTCGGAAAAAGACGGAAAGGAGACTGGCTGGTCAGCATTTTATGTTGATGGCAAATGGGTTGAAGGAAAAAAATAACCTTTAATTCTGTCAGGttttt                                                             >  2:252426/1‑140 (MQ=255)

                                                                           cATGGTTCGCTTTAGCCGTAAAACCAAACAGCAATATGTCTCTTCGGAAAAAGACGGAAAGGAGACTGGCTGGTCAGCATTTTATGTTGATGGCAAATGGGTTGAAGGAAAAAAATAACCTTTAATTCTGTCAGGttttt                                                             >  1:673722/1‑140 (MQ=255)

                                                                           cATGGTTCGCTTTAGCCGTAAAACCAAACAGCAATATGTCTCTTCGGAAAAAGACGGAAAGGAGACTGGCTGGTCAGCATTTTATGTTGATGGCAAATGGGTTGAAGGAAAAAAATAACCTTTAATTCTGTCAGGttttt                                                             >  2:2228424/1‑140 (MQ=255)

                                                                            aTGGTTCGCTTTAGCCGTAAAACCAAACAGCAATATGTCTCTTCGGAAAAAGACGGAAAGGAGACTGGCTGGTCAGCATTTTATGTTGATGGCAAATGGGTTGAAGGAAAAAAATAACCTTTAATTCTGTCAGGTTTTta                                                            >  2:1324795/1‑140 (MQ=255)

                                                                            aTGGTTCGCTTTAGCCGTAAAACCAAACAGCAATATGTCTCTTCGGAAAAAGACGGAAAGGAGACTGGCTGGTCAGCATTTTATGTTGATGGCAAATGGGTTGAAGGAAAAAAATAACCTTTAATTCTGTCAGGTTTTta                                                            >  1:921881/1‑140 (MQ=255)

                                                                            aTGGTTCGCTTTAGCCGTAAAACCAAACAGCAATATGTCTCTTCGGAAAAAGACGGAAAGGAGACTGGCTGGTCAGCATTTTATGTTGATGGCAAATGGGTTGAAGGAAAAAAATAACCTTTAATTCTGTCAGGTTTTta                                                            >  1:1346500/1‑140 (MQ=255)

                                                                            aTGGTTCGCTTTAGCCGTAAAACCAAACAGCAATATGTCTCTTCGGAAAAAGACGGAAAGGAGACTGGCTGGTCAGCATTTTATGTTGATGGCAAATGGGTTGAAGGAAAAAAATAACCTTTAATTCTGTCAGGTTTTta                                                            >  2:408704/1‑140 (MQ=255)

                                                                            aTGGTTCGCTTTAGCCGTAAAACCAAACAGCAATATGTCTCTTCGGAAAAAGACGGAAAGGAGACTGGCTGGTCAGCATTTTATGTTGATGGCAAATGGGTTGAAGGAAAAAAATAACCTTTAATTCTGTCAGGTTTTta                                                            >  1:767132/1‑140 (MQ=255)

                                                                                   gCTTTAGCCGTAAAACCAAACAGCAATATGTCTCTTCGGAAAAAGACGGAAAGGAGACTGGCTGGTCAGCATTTTATGTTGATGGCAAATGGGTTGAAGGAAAAAAATAACCTTTAATTCTGTCAGGTTTTTATAAACaa                                                     >  2:2018758/1‑140 (MQ=255)

                                                                                        aGCCGTAAAACCAAACAGCAATATGTCTCTTCGGAAAAAGACGGAAAGGAGACTGGCTGGTCAGCATTTTATGTTGATGGCAAATGGGTTGAAGGAAAAAAATAACCTTTAATTCTGTCAGGTTTTTATAAACaa                                                     >  2:1922538/1‑135 (MQ=255)

                                                                                        aGCCGTAAAACCAAACAGCAATATGTCTCTTCGGAAAAAGACGGAAAGGAGACTGGCTGGTCAGCATTTTATGTTGATGGCAAATGGGTTGAAGGAAAAAAATAACCTTTAATTCTGTCAGGTTTTTATAAACAAAGGGt                                                >  1:2093172/1‑140 (MQ=255)

                                                                                         gCCGTAAAACCAAACAGCAATATGTCTCTTCGGAAAAAGACGGAAAGGAGACTGGCTGGTCAGCATTTTATGTTGATGGCAAATGGGTTGAAGGAAAAAAATAACCTTTAATTCTGTCAGGTTTTTATAAACAAAGGGTc                                               >  2:2011479/1‑140 (MQ=255)

                                                                                            gTAAAACCAAACAGCAATATGTCTCTTCGGAAAAAGACGGAAAGGAGACTGGCTGGTCAGCATTTTATGTTGATGGCAAATGGGTTGAAGGAAAAAAATAACCTTTAATTCTGTCAGGTTTTTATAAACAAAGGGTcgcg                                            >  1:2113235/1‑140 (MQ=255)

                                                                                             tAAAACCAAACAGCAATATGTCTCTTCGGAAAAAGACGGAAAGGAGACTGGCTGGTCAGCATTTTATGTTGATGGCAAATGGGTTGAAGGAAAAAAATAACCTTTAATTCTGTCAGGTTTTTATAAACAAAGGGTCGCGa                                           >  2:837564/1‑140 (MQ=255)

                                                                                                aaCCAAACAGCAATATGTCTCTTCGGAAAAAGACGGAAAGGAGACTGGCTGGTCAGCATTTTATGTTGATGGCAAATGGGTTGAAGGAAAAAAATAACCTTTAATTCTGTCAGGTTTTTATAAACAAAGGGTCGCGAAAg                                        <  1:2228424/140‑1 (MQ=255)

                                                                                                   cAAACAGCAATATGTCTCTTCGGAAAAAGACGGAAAGGAGACTGGCTGGTCAGCATTTTATGTTGATGGCAAATGGGTTGAAGGAAAAAAATAACCTTTAATTCTGTCAGGTTTTTATAAACAAAGGGTCGCGAAAGCgg                                     <  1:2309340/140‑1 (MQ=255)

                                                                                                   cAAACAGCAATATGTCTCTTCGGAAAAAGACGGAAAGGAGACTGGCTGGTCAGCATTTTATGTTGATGGCAAATGGGTTGAAGGAAAAAAATAACCTTTAATTCTGTCAGGTTTTTATAAACAAAGGGTCGCGAAAGCgg                                     <  1:1449123/140‑1 (MQ=255)

                                                                                                     aaCAGCAATATGTCTCTTCGGAAAAAGACGGAAAGGAGACTGGCTGGTCAGCATTTTATGTTGATGGCAAATGGGTTGAAGGAAAAAAATAACCTTTAATTCTGTCAGGTTTTTATAAACAAAGGGTCGCGAAAGCGGcc                                   >  2:676094/1‑140 (MQ=255)

                                                                                                         gtaaTATGTCTCTTCGGAAAAAGACGGAAAGGAGACTGGCTGGTCAGCATTTTATGTTGATGGCAAATGGGTTGAAGGAAAAAAATAACCTTTAATTCTGTCAGGTTTTTATAAACAAAGGGTCGCGAAAGCGGCCCttt                               >  1:2007184/3‑140 (MQ=255)

                                                                                                         gCAATATGTCTCTTCGGAAAAAGACGGAAAGGAGACTGGCTGGTCAGCATTTTATGTTGATGGCAAATGGGTTGAAGGAAAAAAATAACCTTTAATTCTGTCAGGTTTTTATAAACAAAGGGTCGCGAAAGCGGCCCttt                               >  1:999447/1‑140 (MQ=255)

                                                                                                          cAATATGTCTCTTCGGAAAAAGACGGAAAGGAGACTGGCTGGTCAGCATTTTATGTTGATGGCAAATGGGTTGAAGGAAAAAAATAACCTTTAATTCTGTCAGGTTTTTATAAACAAAGGGTCGCGAAAGCGGCCCtttt                              >  1:1383808/1‑140 (MQ=255)

                                                                                                             tatGTCTCTTCGGAAAAAGACGGAAAGGAGACTGGCTGGTCAGCATTTTATGTTGATGGCAAATGGGTTGAAGGAAAAAAATAACCTTTAATTCTGTCAGGTTTTTATAAACAAAGGGTCGCGAAAGCGGCCCTTTTTTa                           <  1:541317/140‑1 (MQ=255)

                                                                                                              atGTCTCTTCGGAAAAAGACGGAAAGGAGACTGGCTGGTCAGCATTTTATGTTGATGGCAAATGGGTTGAAGGAAAAAAATAACCTTTAATTCTGTCAGGTTTTTATAAACAAAGGGTCGCGAAAGCGGCCCTTTTTTAt                          >  1:199856/1‑140 (MQ=255)

                                                                                                               tGTCTCTTCGGAAAAAGACGGAAAGGAGACTGGCTGGTCAGCATTTTATGTTGATGGCAAATGGGTTGAAGGAAAAAAATAACCTTTAATTCTGTCAGGTTTTTATAAACAAAGGGTCGCGAAAGCGGCCCTTTTTTAtt                         <  2:921881/140‑1 (MQ=255)

                                                                                                               tGTCTCTTCGGAAAAAGACGGAAAGGAGACTGGCTGGTCAGCATTTTATGTTGATGGCAAATGGGTTGAAGGAAAAAAATAACCTTTAATTCTGTCAGGTTTTTATAAACAAAGGGTCGCGAAAGCGGCCCTTTTTTAtt                         >  1:748215/1‑140 (MQ=255)

                                                                                                                   tctTCGGAAAAAGACGGAAAGGAGACTGGCTGGTCAGCATTTTATGTTGATGGCAAATGGGTTGAAGGAAAAAAATAACCTTTAATTCTGTCAGGTTTTTATAAACAAAGGGTCGCGAAAGCGGCCCTTTTTTATTGCat                     <  2:1708660/140‑1 (MQ=255)

                                                                                                                    ctTCGGAAAAAGACGGAAAGGAGACTGGCTGGTCAGCATTTTATGTTGATGGCAAATGGGTTGAAGGAAAAAAATAACCTTTAATTCTGTCAGGTTTTTATAAACAAAGGGTCGCGAAAGCGGccc                                  >  2:49940/1‑126 (MQ=255)

                                                                                                                    ctTCGGAAAAAGACGGAAAGGAGACTGGCTGGTCAGCATTTTATGTTGATGGCAAATGGGTTGAAGGAAAAAAATAACCTTTAATTCTGTCAGGTTTTTATAAACAAAGGGTCGCGAAAGCGGccc                                  <  1:49940/126‑1 (MQ=255)

                                                                                                                    ctTCGGAAAAAGACGGAAAGGAGACTGGCTGGTCAGCATTTTATGTTGATGGCAAATGGGTTGAAGGAAAAAAATAACCTTTAATTCTGTCAGGTTTTTATAAACAAAGGGTCGCGAAAGCGGCCCTTTTTTATTGCata                    >  2:297518/1‑140 (MQ=255)

                                                                                                                     ttCGGAAAAAGACGGAAAGGAGACTGGCTGGGCAGCATTTTATGTTGATGGCAAATGGGATGAAGGAAAAAAATCACCTTTAATTCTGTCAGGTTTTTATAAACAAAGGGACGCGGAAGCGGCCCTTTTTTATTGCttat                   >  2:542292/1‑140 (MQ=255)

                                                                                                                      tCGGAAAAAGACGGAAAGGAGACTGGCTGGTCAGCATTTTATGTTGATGGCAAATGGGTTGAAGGAAAAAAATAACCTTTAATTCTGTCAGGTTTTTATAAACAAAGGGTCGCGAAAGCGGCCCTTTTTTATTGCAtatt                  >  2:1845348/1‑140 (MQ=255)

                                                                                                                       cGGAAAAAGACGGAAAGGAGACTGGCTGGTCAGCATTTTATGTTGATGGCAAATGGGTTGAAGGAAAAAAATAACCTTTAATTCTGTCAGGTTTTTATAAACAAAGGGTCGCGAAAGCGGCCCTTTTTTATTGCAtatta                 >  2:2072722/1‑140 (MQ=255)

                                                                                                                       cGGAAAAAGACGGAAAGGAGACTGGCTGGTCAGCATTTTATGTTGATGGCAAATGGGTTGAAGGAAAAAAATAACCTTTAATTCTGTCAGGTTTTTATAAACAAAGGGTCGCGAAAGCGGCCCTTTTTTATTGCAtatta                 >  2:501115/1‑140 (MQ=255)

                                                                                                                       cGGAAAAAGACGGAAAGGAGACTGGCTGGTCAGCATTTTATGTTGATGGCAAATGGGTTGAAGGAAAAAAATAACCTTTAATTCTGTCAGGTTTTTATAAACAAAGGGTCGCGAAAGCGGCCCTTTTTTATTGCAtatta                 >  1:1219491/1‑140 (MQ=255)

                                                                                                                       cGGAAAAAGACGGAAAGGAGACTGGCTGGTCAGCATTTTATGTTGATGGCAAATGGGTTGAAGGAAAAAAATAACCTTTAATTCTGTCAGGTTTTTATAAACAAAGGGTCGCGAAAGCGGCCCTTTTTTATTGCAtatta                 >  1:1866738/1‑140 (MQ=255)

                                                                                                                       cGGAAAAAGACGGAAAGGAGACTGGCTGGTCAGCATTTTATGTTGATGGCAAATGGGTTGAAGGAAAAAAATAACCTTTAATTCTGTCAGGTTTTTATAAACAAAGGGTCGCGAAAGCGGCCCTTTTTTATTGCAtatta                 >  2:863337/1‑140 (MQ=255)

                                                                                                                               gACGGAAAGGAGACTGGCTGGTCAGCATTTTATGTTGATGGCAAATGGGTTGAAGGAAAAAAATAACCTTTAATTCTGTCAGGTTTTTATAAACAAAGGGTCGCGAAAGCGGCCCTTTTTTATTGCATATTATTTTttct         <  1:1232585/140‑1 (MQ=255)

                                                                                                                                aCGGAAAGGAGACTGGCTGGTCAGTATTTTATGTTGATGGCAAATGGGTTGAAGGAAAAAAATAACCTTTCAGTCTGTCAGGTTTTTATAAACAAAGGGTCGCGAAAGCGGCCCTTTATTATTGCATATTATTTTttcgt        <  2:1844721/140‑3 (MQ=255)

                                                                                                                                aCGGAAAGGAGACTGGCTGGTCAGCATTTTATGTTGATGGCAAATGGGTTGAAGGAAAAAAATAACCTTTAATTCTGTCAGGTTTTTATAAACAAAGGGTCGCGAAAGCGGCCCTTTTTTATTGCATATTATTTTttctt        <  1:252426/140‑1 (MQ=255)

                                                                                                                                aCGGAAAGGAGACTGGCTGGTCAGCATTTTATGTTGATGGCAAATGGGTTGAAGGAAAAAAATAACCTTTAATTCTGTCAGGTTTTTATAAACAAAGGGTCGCGAAAGCGGCCCTTTTTTATTGCATATTATTTTttctt        <  1:623921/140‑1 (MQ=255)

                                                                                                                                aCGGAAAGGAGACTGGCTGGTCAGCATTTTATGTTGATGGCAAATGGGTTGAAGGAAAAAAATAACCTTTAATTCTGTCAGGTTTTTATAAACAAAGGGTCGCGAAAGCGGCCCTTTTTTATTGCATATTATTTTttctt        <  1:1121002/140‑1 (MQ=255)

                                                                                                                                aCGGAAAGGAGACTGGCTGGTCAGCATTTTATGTTGATGGCAAATGGGTTGAAGGAAAAAAATAACCTTTAATTCTGTCAGGTTTTTATAAACAAAGGGTCGCGAAAGCGGCCCTTTTTTATTGCATATTATTTTttctt        <  1:408704/140‑1 (MQ=255)

                                                                                                                                  ggAAAGGAGACTGGCTGGTCAGCATTTTATGTTGATGGCAAATGGGTTGAAGGAAAAAAATAACCTTTAATTCTGTCAGGTTTTTATAAACAAAGGGTCGCGAAAGCGGCCCTTTTTTATTGCATATTATTTTTTCTTca      <  2:586915/140‑1 (MQ=255)

                                                                                                                                  ggAAAGGAGACTGGCTGGTCAGCATTTTATGTTGATGGCAAATGGGTTGAAGGAAAAAAATAACCTTTAATTCTGTCAGGTTTTTATAAACAAAGGGTCGCGAAAGCGGCCCTTTTTTATTGCATATTATTTTTTCTTca      <  1:1718094/140‑1 (MQ=255)

                                                                                                                                  ggAAAGGAGACTGGCTGGTCAGCATTTTATGTTGATGGCAAATGGGTTGAAGGAAAAAAATAACCTTTAATTCTGTCAGGTTTTTATAAACAAAGGGTCGCGAAAGCGGCCCTTTTTTATTGCATATTATTTTTTCTTca      <  2:748215/140‑1 (MQ=255)

                                                                                                                                  ggAAAGGAGACTGGCTGGTCAGCATTTTATGTTGATGGCAAATGGGTTGAAGGAAAAAAATAACCTTTAATTCTGTCAGGTTTTTATAAACAAAGGGTCGCGAAAGCGGCCCTTTTTTATTGCATATTATTTTTTCTTca      <  1:1490545/140‑1 (MQ=255)

                                                                                                                                  ggAAAGGAGACTGGCTGGTCAGCATTTTATGTTGATGGCAAATGGGTTGAAGGAAAAAAATAACCTTTAATTCTGTCAGGTTTTTATAAACAAAGGGTCGCGAAAGCGGCCCTTTTTTATTGCATATTATTTTTTCTTca      <  1:2018758/140‑1 (MQ=255)

                                                                                                                                  ggAAAGGAGACTGGCTGGTCAGCATTTTATGTTGATGGCAAATGGGTTGAAGGAAAAAAATAACCTTTAATTCTGTCAGGTTTTTATAAACAAAGGGTCGCGAAAGCGGCCCTTTTTTATTGCATATTATTTTTTCTTca      <  1:676094/140‑1 (MQ=255)

                                                                                                                                  ggAAAGGAGACTGGCTGGTCAGCATTTTATGTTGATGGCAAATGGGTTGAAGGAAAAAAATAACCTTTAATTCTGTCAGGTTTTTATAAACAAAGGGTCGCGAAAGCGGCCCTTTTTTATTGCATATTATTTTTTCTTca      <  1:940737/140‑1 (MQ=255)

                                                                                                                                  ggAAAGGAGACTGGCTGGTCAGCATTTTATGTTGATGGCAAATGGGTTGAAGGAAAAAAATAACCTTTAATTCTGTCAGGTTTTTATAAACAAAGGGTCGCGAAAGCGGCCCTTTTTTATTGCATATTATTTTTTCTTca      <  2:1746130/140‑1 (MQ=255)

                                                                                                                                  ggAAAGGAGACTGGCTGGTCAGCATTTTATGTTGATGGCAAATGGGTTGAAGGAAAAAAATAACCTTTAATTCTGTCAGGTTTTTATAAACAAAGGGTCGCGAAAGCGGCCCTTTTTTATTGCATATTATTTTTTCTTca      <  2:1616870/140‑1 (MQ=255)

                                                                                                                                  ggAAAGGAGACTGGCTGGTCAGCATTTTATGTTGATGGCAAATGGGTTGAAGGAAAAAAATAACCTTTAATTCTGTCAGGTTTTTATAAACAAAGGGTCGCGAAAGCAGCCCTTTTTTATTGCATATTATTTTTTCTTca      <  2:1772721/140‑1 (MQ=255)

                                                                                                                                    aaaGGAGACTGGCTGGTCAGCATTTTATGTTGATGGCAAATGGGTTGAAGGAAAAAAATAACCTTTAATTCTGTCAGGTTTTTATAAACAAAGGGTCGCGAAAGCGGCCCTTTTTTATTGCATATTATTTTTTCTTcaca    <  2:142879/140‑1 (MQ=255)

                                                                                                                                     aaGGAGACTGGCTGGTCAGCATTTTATGTTGATGGCAAATGGGTTGAAGGAAAAAAATAACCTTTAATTCTGTCAGGTTTTTATAAACAAAGGGTCGCGAAAGCGGCCCTTTTTTATTGCATATTATTTTTTCTTCacac   <  1:1324795/140‑1 (MQ=255)

                                                                                                                                     aaGGAGACTGGCTGGTCAGCATTTTATGTTGATGGCAAATGGGTTGAAGGAAAAAAATAACCTTTAATTCTGTCAGGTTTTTATAAACAAAGGGTCGCGAAAGCGGCCCTTTTTTATTGCATATTATTTTTTCTTCacac   <  2:1153599/140‑1 (MQ=255)

                                                                                                                                     aaGGAGACTGGCTGGTCAGCATTTTATGTTGATGGCAAATGGGTTGAAGGAAAAAAATAACCTTTAATTCTGTCAGGTTTTTATAAACAAAGGGTCGCGAAAGCGGCCCTTTTTTATTGCATATTATTTTTTCTTCacac   <  1:1752192/140‑1 (MQ=255)

                                                                                                                                      aGGAGACTGGCTGGTCAGCATTTTATGTTGATGGCAAATGGGTTGAAGGAAAAAAATAACCTTTAATTCTGTCAGGTTTTTATAAACAAAGGGTCGCGAAAGCGGCCCTTTTTTATTGCATATTATTTTTTCTTCACAcc  <  2:673722/140‑1 (MQ=255)

                                                                                                                                      aGGAGACTGGCTGGTCAGCATTTTATGTTGATGGCAAATGGGTTGAAGGAAAAAAATAACCTTTAATTCTGTCAGGTTTTTATAAACAAAGGGTCGCGAAAGCGGCCCTTTTTTATTGCATATTATTTTTTCTTCACAcc  <  1:1253641/140‑1 (MQ=255)

CTTCCGCGACCGTCTGCCGGAAAAACTGCGTTATCTGGCCGATGCGCCACAGCAGGATCCGGAAGGTAATAAGACCATGGTTCGCTTTAGCCGTAAAACCAAACAGCAATATGTCTCTTCGGAAAAAGACGGAAAGGCGACTGGCTGGTCAGCATTTTATGTTGATGGCAAATGGGTTGAAGGAAAAAAATAACCTTTAATTCTGTCAGGTTTTTATAAACAAAGGGTCGCGAAAGCGGCCCTTTTTTATTGCATATTATTTTTTCTTCACACC  >  NC_000913/1333453‑1333726

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 24 ≤` `ATCG/ATCG` `< 31 ≤` `ATCG/ATCG` `< 35 ≤` `ATCG/ATCG` `< 41 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`