Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	2,285,185	G→T	L270L (CTG→CTT)	yejM →	putative cardiolipin transport protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	2,285,185	0	G	T	100.0%	68.2 / NA	21	L270L (CTG→CTT)	yejM	putative cardiolipin transport protein
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base T (9/12);  total (9/12)

TGCGCAGGAGTATCAACGCCGTCTTATTGAGCAAGGTAATCCAGACGCCGTTTCCGTTCAGTATCCGTTAAGCGAACTGCGCTATCGCGATATGGGCACCGGGCAGAATGTGCTGTTGATTACTGTCGATGGCCTGAACTACTCACGCTTCGAGAAGCAGATGCCTGCGCTGGCAGGTTTTGCTGAGCAAAATATTTCGTTCACGCGCCATATGAGCTCCGGCAACACTACAGACAACGGCATCTTTGGCCTGTTCTATGGCATCTCGCCG  >  NC_000913/2285050‑2285320                                                                                                                                        |                                                                                                                                        tGCGCAGGAGTATCAACGCCGTCTTATTGAGCAAGGTAATCCAGACGCCGTTTCCGTTCAGTATCCGTTAAGCGAACTGCGCTATCGCGATATGGGCACCGGGCAGAATGTGCTGTTGATTACTGTCGATGGCCTTAact                                                                                                                                     >  1:280151/1‑140 (MQ=255)    gcAGGAGTATCAACGCCGTCTTATTGAGCAAGGTAATCCAGACGCCGTTTCCGTTCAGTATCCGTTAAGCGAACTGCGCTATCGCGATATGGGCACCGGGCAGAATGTGCTGTTGATTACTGTCGATGGCCTTAactact                                                                                                                                  <  2:287024/140‑1 (MQ=255)           tATCAACGCCGTCTTATTGAGCAAGGTAATCCAGACGCCGTTTCCGTTCAGTATCCGTTAAGCGAACTGCGCTATCGCGATATGGGCACCGGGCAGAATGTGCTGTTGATTACTGTCGATGGCCTTAACTACTCACGCtt                                                                                                                           <  1:96582/140‑1 (MQ=255)               aaCGCCGTCTTATTGAGCAAGGTAATCCAGACGCCGTTTCCGTTCAGTATCCGTTAAGCGAACTGCGCTATCGCGATATGGGCACCGGGCAGAATGTGCTGTTGATTACTGTCGATGGCCTTAACTACTCACGCTTCgag                                                                                                                       <  2:193517/140‑1 (MQ=255)                       cTTATTGAGCAAGGTAATCCAGACGCCGTTTCCGTTCAGTATCCGTTAAGCGAACTGCGCTATCGCGATATGGGCACCGGGCAGAATGTGCTGTTGATTACTGTCGATGGCCTTAACTACTCACGCTTCGAGAAGCAGAt                                                                                                               >  2:1901/1‑140 (MQ=255)                              aGCAAGGTAATCCAGACGCCGTTTCCGTTCAGTATCCGTTAAGCGAACTGCGCTATCGCGATATGGGCACCGGGCAGAATGTGCTGTTGATTACTGTCGATGGCCTTAACTACTCACGCTTCGAGAAGCAGATGCCTgcg                                                                                                        <  2:280151/140‑1 (MQ=255)                                             aCGCCGTTTCCGTTCAGTATCCGTTAAGCGAACTGCGCTATCGCGATATGGGCACCGGGCAGAATGTGCTGTTGATTACTGTCGATGGCCTTAACTACTCACGCTTCGAGAAGCAGATGCCTGCGCTGGCAGGTTTTGCt                                                                                         >  1:134111/1‑140 (MQ=255)                                                                                cgcTATCGCGATATGGGCACCGGGCAGAATGTGCTGTTGATTACTGTCGATGGCCTTAACTACTCACGCTTCGAGAAGCAGATGCCTGCGCTGGCAGGTTTTGCTGAGCAAAATATTTCGTTCACGCGCCATATGAGCTc                                                      >  1:51894/1‑140 (MQ=255)                                                                                      cgcgATATGGGCACCGGGCAGAATGTGCTGTTGATTACTGTCGATGGCCTTAACTACTCACGCTTCGAGAAGCAGATGCCTGCGCTGGCAGGTTTTGCTGAGCAAAATATTTCGTTCACGCGCCATATGAGCTCCGGCaa                                                <  2:183097/140‑1 (MQ=255)                                                                                                    cGGGCAGAATGTGCTGTTGATTACTGTCGATGGCCTTAACTACTCACGCTTCGAGAAGCAGATGCCTGCGCTGGCAGGTTTTGCTGAGCAAAATATTTCGTTCACGCGCCATATGAGCTCCGGCAACACTACAGACAACg                                  <  1:117547/140‑1 (MQ=255)                                                                                                     gggCAGAATGTGCTGTTGATTACTGTCGATGGCCTTAACTACTCACGCTTCGAGAAGCAGATGCCTGCGCTGGCAGGTTTTGCTGAGCAAAATATTTCGTTCACGCGCCATATGAGCTCCGGCAACACTACAGACAACgg                                 <  2:199653/140‑1 (MQ=255)                                                                                                      ggCAGAATGTGCTGTTGATTACTGTCGATGGCCTTAACTACTCACGCTTCGAGAAGCAGATGCCTGCGCTGGCAGGTTTTGCTGAGCAAAATATTTCGTTCACGCGCCATATGAGCTCCGGCAACACTACAGACAACggt                                >  2:213123/1‑139 (MQ=255)                                                                                                           aaTGTGCTGTTGATTACTGTCGATGGCCTTAACTACTCACGCTTCGAGAAGCAGATGCCTGCGCTGGCAGGTTTTGCTGAGCAAAATATTTCGTTCACGCGCCATATGAGCTCCGGCAACACTACAGACAACGGCATCtt                           >  1:76647/1‑140 (MQ=255)                                                                                                               tgCTGTTGATTACTGTCGATGGCCTTAACTACTCACGCTTCGAGAAGCAGATGCCTGCGCTGGCAGGTTTTGCTGAGCAAAATATTTCGTTCACGCGCCATATGAGCTCCGGCAACACTACAGACAACGGCATCTTTGGc                       <  2:213846/140‑1 (MQ=255)                                                                                                               tgCTGTTGATTACTGTCGATGGCCTTAACTACTCACGCTTCGAGAAGCAGATGCCTGCGCTGGCAGGTTTTGCTGAGCAAAATATTTCGTTCACGCGCCATATGAGCTCCGGCAACACTACAGACAACGGCATCTTTGGc                       <  1:1901/140‑1 (MQ=255)                                                                                                                     tGATTACTGTCGATGGCCTTAACTACTCACGCTTCGAGAAGCAGATGCCTGCGCTGGCAGGTTTTGCTGAGCAAAATATTTCGTTCACGCGCCATATGAGCTCCGGCAACACTACAGACAACGGCATCTTTGGCCTGTTc                 >  2:91269/1‑140 (MQ=255)                                                                                                                            tGTCGATGGCCTTAACTACTCACGCTTCGAGAAGCAGATGCCTGCGCTGGCAGGTTTTGCTGAGCAAAATATTTCGTTCACGCGCCATATGAGCTCCGGCAACACTACAGACAACGGCATCTTTGGCCTGTTCTATGGCa          >  2:141457/1‑140 (MQ=255)                                                                                                                                gATGGCCTTAACTACTCACGCTTCGAGAAGCAGATGCCTGCGCTGGCAGGTTTTGCTGAGCAAAATATTTCGTTCACGCGCCATATGAGCTCCGGCAACACTACAGACAACGGCATCTTTGGCCTGTTCTATGGCAtctc      >  2:221809/1‑140 (MQ=255)                                                                                                                                 aTGGCCTTAACTACTCACGCTTCGAGAAGCAGATGCCTGCGCTGGCAGGTTTTGCTGAGCAAAATATTTCGTTCACGCGCCATATGAGCTCCGGCAACACTACAGACAACGGCATCTTTGGCCTGTTCTATGGCATCTCg     <  1:158572/140‑1 (MQ=255)                                                                                                                                    gCCTTAACTACTCACGCTTCGAGAAGCAGATGCCTGCGCTGGCAGGTTTTGCTGAGCAAAATATTTCGTTCACGCGCCATATGAGCTCCGGCAACACTACAGACAACGGCATCTTTGGCCTGTTCTATGGCATCTCGCCg  <  1:193723/140‑1 (MQ=255)                                                                                                                                    gCCTTAACTACTCACGCTTCGAGAAGCAGATGCCTGCGCTGGCAGGTTTTGCTGAGCAAAATATTTCGTTCACGCGCCATATGAGCTCCGGCAACACTACAGACAACGGCATCTTTGGCCTGTTCTATGGCATCTCGCCg  <  2:51894/140‑1 (MQ=255)                                                                                                                                        |                                                                                                                                        TGCGCAGGAGTATCAACGCCGTCTTATTGAGCAAGGTAATCCAGACGCCGTTTCCGTTCAGTATCCGTTAAGCGAACTGCGCTATCGCGATATGGGCACCGGGCAGAATGTGCTGTTGATTACTGTCGATGGCCTGAACTACTCACGCTTCGAGAAGCAGATGCCTGCGCTGGCAGGTTTTGCTGAGCAAAATATTTCGTTCACGCGCCATATGAGCTCCGGCAACACTACAGACAACGGCATCTTTGGCCTGTTCTATGGCATCTCGCCG  >  NC_000913/2285050‑2285320

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 24 ≤ ATCG/ATCG < 31 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑