Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	2,469,105	A→T	K298* (AAA→TAA)	yfdH →	CPS‑53 (KpLE1) prophage; bactoprenol glucosyl transferase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	2,469,105	0	A	T	100.0%	72.1 / NA	22	K298* (AAA→TAA)	yfdH	CPS‑53 (KpLE1) prophage; bactoprenol glucosyl transferase
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base T (16/6);  total (16/6)

TATCATATTTGGAAATGCTGTTAGGGGATATCCTTCACTACTTGTTTCAATACTGTTTTTAGGTGGAATTCAGATGATTGGAATAGGAGTATTAGGTGAATATATTGGACGCACATACATTGAAACCAAAAAACGCCCGAAATACATCATCAAGAGAGTCAAAAAATGAATAAAGCAATAAAAGTATCATTGTATATATCTTTTGTTTTGATTATTTGCGCCTTATCTAAAAACATAATGATGTTAAATACATCTGATTTCGGAAGAGCCATT  >  NC_000913/2468966‑2469238                                                                                                                                            |                                                                                                                                      tATCATATTTGGAAATGCTGTTAGGGGATATCCTTCACTACTTGTTTCAATACTGTTTTTAGGTGGAATTCAGATGATTGGAATAGGAGTATTAGGTGAATATATTGGACGCACATACATTGAAACCAAAAAACGCCCGa                                                                                                                                       >  2:158022/1‑140 (MQ=255)           ggAAATGCTGTTAGGGGATATCCTTCACTACTTGTTTCAATACTGTTTTTAGGTGGAATTCAGATGATTGGAATAGGAGTATTAGGTGAATATATTGGACGCACATACATTGAAACCAAAAAACGCCCGTAATAcatcat                                                                                                                             >  2:19091/1‑140 (MQ=255)                     ttAGGGGATATCCTTCACTACTTGTTTCAATACTGTTTTTAGGTGGAATTCAGATGATTGGAATAGGAGTATTAGGTGAATATATTGGACGCACATACATTGAAACCAAAAAACGCCCGTAATACATCATCAAGAGAGTc                                                                                                                   >  1:91234/1‑140 (MQ=255)                            atatCCTTCACTACTTGTTTCAATACTGTTTTTAGGTGGAATTCAGATGATTGGAATAGGAGTATTAGGTGAATATATTGGACGCACATACATTGAAACCAAAAAACGCCCGTAATACATCATCAAGAGAGTCAAAAAAt                                                                                                            >  1:209773/1‑140 (MQ=255)                             tatCCTTCACTACTTGTTTCAATACTGTTTTTAGGTGGAATTCAGATGATTGGAATAGGAGTATTAGGTGAATATATTGGACGCACATACATTGAAACCAAAAAACGCCCGTAATACATCATCAAGAGAGTCaaaaa                                                                                                              <  2:40475/137‑1 (MQ=255)                             tatCCTTCACTACTTGTTTCAATACTGTTTTTAGGTGGAATTCAGATGATTGGAATAGGAGTATTAGGTGAATATATTGGACGCACATACATTGAAACCAAAAAACGCCCGTAATACATCATCAAGAGAGTCaaaaa                                                                                                              >  1:40475/1‑137 (MQ=255)                               tCCTTCACTACTTGTTTCAATACTGTTTTTAGGTGGAATTCAGATGATTGGAATAGGAGTATTAGGTGAATATATTGGACGCACATACATTGAAACCAAAAAACGCCCGTAATACATCATCAAGAGAGTCAAAAAATGaa                                                                                                         >  1:115148/1‑140 (MQ=255)                               tCCTTCACTACTTGTTTCAATACTGTTTTTAGGTGGAATTCAGATGATTGGAATAGGAGTATTAGGTGAATATATTGGACGCACATACATTGAAACCAAAAAACGCCCGTAATACATCATCAAGAGAGTCAAAAAATGaa                                                                                                         >  1:268521/1‑140 (MQ=255)                                   tCACTACTTGTTTCAATACTGTTTTTAGGTGGAATTCAGATGATTGGAATAGGAGTATTAGGTGAATATATTGGACGCACATACATTGAAACCAAAAAACGCCCGTAATACATCATCAAGAGAGTCAAAAAATGAATaaa                                                                                                     >  1:317127/1‑140 (MQ=255)                                            gTTTCAATACTGTTTTTAGGTGGAATTCAGATGATTGGAATAGGAGTATTAGGTGAATATATTGGACGCACATACATTGAAACCAAAAAACGCCCGTAATACATCATCAAGAGAGTCAAAAAATGAATAAAGCAATaaaa                                                                                            <  1:98087/140‑1 (MQ=255)                                               tCAATACTGTTTTTAGGTGGAATTCAGATGATTGGAATAGGAGTATTAGGTGAATATATTGGACGCACATACATTGAAACCAAAAAACGCCCGTAATACATCATCAAGAGAGTCAAAAAATGAATAAAGCAATAAAAGTa                                                                                         >  1:39019/1‑140 (MQ=255)                                                  aTACTGTTTTTAGGTGGAATTCAGATGATTGGAATAGGAGTATTAGGTGAATATATTGGACGCACATACATTGAAACCAAAAAACGCCCGTAATACATCATCAAGAGAGTCAAAAAATGAATAAAGCAATAAAAGTATCa                                                                                      <  2:115148/140‑1 (MQ=255)                                                      tGTTTTTAGGTGGAATTCAGATGATTGGAATAGGAGTATTAGGTGAATATATTGGACGCACATACATTGAAACCAAAAAACGCCCGTAATACATCATCAAGAGAGTCAAAAAATGAATAAAGCAATAACAGTATCATTGt                                                                                  >  2:45086/1‑140 (MQ=255)                                                        tttttAGGTGGAATTCAGATGATTGGAATAGGAGTATTAGGTGAATATATTGGACGCACATACATTGAAACCAAAAAACGCCCGTAATACATCATCAAGAGAGCCAAAAAATGAATAAAGCAATAAAAGTATCATTGtat                                                                                >  2:91001/1‑140 (MQ=255)                                                                                       gAGTATTAGGTGAATATATTGGACGCACATACATTGAAACCAAAAAACGCCCGTAATACATCATCAAGAGAGTCAAAAAATGAATAAAGCAATAAAAGTATCATTGTATATATCTTTTGTTTTGATTATTTGCGCCTTAt                                                 >  2:56086/1‑140 (MQ=255)                                                                                            ttAGGTGAATATATTGGACGCACATACATTGAAACCAAAAAACGCCCGTAATACATCATCAAGAGAGTCAAAAAATGAATAAAGCAATAAAAGTATCATTGTATATATCTTTTGTTTTGATTATTTGCGCCTTATCTaaa                                            >  2:153230/1‑140 (MQ=255)                                                                                                   aaTATATTGGACGCACATACATTGAAACCAAAAAACGCCCGTAATACATCATCAAGAGAGTCAAAAAATGAATAAAGCAATAAAAGTATCATTGTATATATCTTTTGTTTTGATTATTTGCGCCTTATCTAAAAACATaa                                     <  2:268521/140‑1 (MQ=255)                                                                                                          tGGACGCACATACATTGAAACCAAAAAACGCCCGTAATACATCATCAAGAGAGTCAAAAAATGAATAAAGCAATAAAAGTATCATTGTATATATCTTTTGTTTTGATTATTTGCGCCTTATCTAAAAACATAATGATGtt                              >  1:114983/1‑140 (MQ=255)                                                                                                             aCGCACATACATTGAAACCAAAAAACGCCCGTAATACATCATCAAGAGAGTCAAAAAATGAATAAAGCAATAAAAGTATCATTGTATATATCTTTTGTTTTGATTATTTGCGCCTTATCTAAAAACATAATGATGTTaaa                           <  1:98300/140‑1 (MQ=255)                                                                                                              cGCACATACATTGAAACCAAAAAACGCCCGTAATACATCATCAAGAGAGTCAAAAAATGAATAAAGCAATAAAAGTATCATTGTATATATCTTTTGTTTTGATTATTTGCGCCTTATCTAAAAACATAATGATGTTAAAt                          >  2:4764/1‑140 (MQ=255)                                                                                                                 acatacatTGAAACCAAAAAACGCCCGTAATACATCATCAAGAGAGTCAAAAAATGAATAAAGCAATAAAAGTATCATTGTATATATCTTTTGTTTTGATTATTTGCGCCTTATCTAAAAACATAATGATGTTAAATACa                       >  2:276818/1‑140 (MQ=255)                                                                                                                               cAAAAAACGCCCGTAATACATCATCAAGAGAGTCAAAAAATGAATAAAGCAATAAAAGTATCATTGTATATATCTTTTGTTTTGATTATTTGCGCCTTATCTAAAAACATAATGATGTTAAATACATCTGATTTCGGAag         >  2:14430/1‑140 (MQ=255)                                                                                                                                      cGCCCGTAATACATCATCAAGAGAGTCAAAAAATGAATAAAGCAATAAAAGTATCATTGTATATATCTTTTGTTTTGATTATTTGCGCCTTATCTAAAAACATAATGATGTTAAATACATCTGATTTCGGAAGagccatt  <  1:19091/140‑1 (MQ=255)                                                                                                                                            |                                                                                                                                      TATCATATTTGGAAATGCTGTTAGGGGATATCCTTCACTACTTGTTTCAATACTGTTTTTAGGTGGAATTCAGATGATTGGAATAGGAGTATTAGGTGAATATATTGGACGCACATACATTGAAACCAAAAAACGCCCGAAATACATCATCAAGAGAGTCAAAAAATGAATAAAGCAATAAAAGTATCATTGTATATATCTTTTGTTTTGATTATTTGCGCCTTATCTAAAAACATAATGATGTTAAATACATCTGATTTCGGAAGAGCCATT  >  NC_000913/2468966‑2469238

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 31 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑