Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	2,830,359	G→T	A183S (GCC→TCC)	gutQ →	D‑arabinose 5‑phosphate isomerase GutQ

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	2,830,359	0	G	T	100.0%	29.9 / NA	11	A183S (GCC→TCC)	gutQ	D‑arabinose 5‑phosphate isomerase GutQ
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base T (6/5);  total (6/5)

ATCTCCGTAGAACGCGAAGCCTGCCCGATGCACCTTGCGCCGACCTCCAGCACCGTCAATACCCTGATGATGGGTGACGCGCTGGCGATGGCGGTCATGCAGGCGCGCGGATTTAATGAAGAAGATTTTGCCCGCTCCCACCCAGCCGGGGCACTGGGCGCTCGCTTGCTGAATAAAGTGCATCATCTGATGCGCCGTGACGATGCCATCCCACAGGTGGCGTTAACCGCCAGCGTG  >  NC_000913/2830230‑2830466                                                                                                                                  |                                                                                                            atCTCCGTAGAACGCGAAGCCTGCCCGATGCACCTTGCGCCGACCTCCAGCACCGTCAATACCCTGATGATGGGTGACGCGCTGGCGATGGCGGTCATGCAGGCGCGCGGATTTAATGAAGAAGATTTTTCCCGCTccca                                                                                                   >  2:253221/1‑140 (MQ=255)         aGAACGCGAAGCCTGCCCGATGCACCTTGCGCCGACCTCCAGCACCGTCAATACCCTGATGATGGGTGACGCGCTGGCGATGGCGGTCATGCAGGCGCGCGGATTTAATGAAGAAGATTTTTCCCGCTCCCACCCAGCCg                                                                                           <  1:209944/140‑1 (MQ=255)                          cGATGCACCTTGCGCCGACCTCCAGCACCGTCAATACCCTGATGATGGGTGACGCGCTGGCGATGGCGGTCATGCAGGCGCGCGGATTTAATGAAGAAGATTTTTCCCGCTCCCACCCAGCCGGGGCACTGGGcgctcgc                                                                          <  1:253221/140‑1 (MQ=255)                            aTGCACCTTGCGCCGACCTCCAGCACCGTCAATACCCTGATGATGGGTGACGCGCTGGCGATGGCGGTCATGCAGGCGCGCGGATTTAATGAAGAAGATTTTTCCCGCTCCCACCCAGCCGGGGCACTGGGCGCTCGCtt                                                                        >  1:30945/1‑140 (MQ=255)                                                   cACCGTCAATACCCTGATGATGGGTGACGCGCTGGCGATGGCGGTCATGCAGGCGCGCGGATTTAATGAAGAAGATTTTTCCCGCTCCCACCCAGCCGGGGCACTGGGCGCTCGCTTGCTGAATAAAGTGCATCATCTGa                                                 <  2:30945/140‑1 (MQ=255)                                                                        gggTGACGCGCTGGCGATGGCGGTCATGCAGGCGCGCGGATTTAATGAAGAAGATTTTTCCCGCTCCCACCCAGCCGGGGCACTGGGCGCTCGCTTGCTGAATAAAGTGCATCATCTGATGCGCCGTGACGATGCCATcc                            >  2:26151/1‑140 (MQ=255)                                                                         ggTGACGCGCTGGCGATGGCGGTCATGCAGGCGCGCGGATTTAATGAAGAAGATTTTTCCCGCTCCCACCCAGCCGGGGCACTGGGCGCTCGCTTGCTGAATAAAGTGCATCATCTGATGCGCCGTGACGATGCCATccc                           >  2:104206/1‑140 (MQ=255)                                                                         ggTGACGCGCTGGCGATGGCGGTCATGCAGGCGCGCGGATTTAATGAAGAAGATTTTTCCCGCTCCCACCCAGCCGGGGCACTGGGCGCTCGCTTGCTGAATAAAGAGCATCATCTGATGCGCCGTGACGATGCCATccc                           >  1:307819/1‑140 (MQ=255)                                                                                   tGGCGATGGCGGTCATGCAGGCGCGCGGATTTAATGAAGAAGATTTTTCCCGCTCCCACCCAGCCGGGGCACTGGGCGCTCGCTTGCTGAATAAAGTGCATCATCTGATGCGCCGTGACGATGCCATCCCACAGGTGGCg                 >  1:274763/1‑140 (MQ=255)                                                                                            cGGTCATGCAGGCGCGCGGATTTAATGAAGAAGATTTTTCCCGCTCCCCCCCAGCCGGGGCACTGGGCGCTCGCTTGCTGAATAAAGTGCATCATCTGATGCGCCGTGACGATGCCATCCCACAGGTGGCGTTAACCGcc        <  1:104206/140‑1 (MQ=255)                                                                                                  tGCAGGCGCGCGGATTTAATGAAGAAGATTTTTCCCGCTCCCACCCAGCCGGGGCACTGGGCGCTCGCTTGCTGAATAAAGTGCATCATCTGATGCGCCGTGACGATGCCATCCCACAGGTGGCGTTAACCGCCAGCGTg  <  1:26151/140‑1 (MQ=255)                                                                                                                                  |                                                                                                            ATCTCCGTAGAACGCGAAGCCTGCCCGATGCACCTTGCGCCGACCTCCAGCACCGTCAATACCCTGATGATGGGTGACGCGCTGGCGATGGCGGTCATGCAGGCGCGCGGATTTAATGAAGAAGATTTTGCCCGCTCCCACCCAGCCGGGGCACTGGGCGCTCGCTTGCTGAATAAAGTGCATCATCTGATGCGCCGTGACGATGCCATCCCACAGGTGGCGTTAACCGCCAGCGTG  >  NC_000913/2830230‑2830466

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑