Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	262,942	C→A	noncoding (72/76 nt)	thrW →	tRNA‑Thr

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	262,942	0	C	A	100.0%	88.5 / NA	26	noncoding (72/76 nt)	thrW	tRNA‑Thr
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base A (9/17);  total (9/17)

GCCCGGTTAGTTTTAACCTTGTCCACCGTGATTCACGTTCGTGAACATGTCCTTTCAGGGCCGATATAGCTCAGTTGGTAGAGCAGCGCATTCGTAATGCGAAGGTCGTAGGTTCGACTCCTATTATCGGCACCATTAAAATCAAATTGTTACGTAAGATCTTATCATTCTCCCACCAAAAAATTATCTTAATGTAACAGCTGGTGTAAGTAAATTCTATCAACGAAGATCAATCTTATCTACTGACCAAAAAGGCCTGATAGGGCT  >  NC_000913/262812‑263078                                                                                                                                   |                                                                                                                                         gCCCGGTTAGTTTTAACCTTGTCCACCGTGATTCACGTTCGTGAACATGTCCTTTCAGGGCCGATATAGCTCAGTTGGTAGAGCAGCGCATTCGTAATGCGAAGGTCGTAGGGTCGACTCCTATTATCGGAACCATTaaa                                                                                                                                 >  1:103191/1‑140 (MQ=255)           ttttAACCTTGTTCACCGTGATTCACGTTCGTGAACATGTCCTTTCAGGGCCGATATAGCTCAGTTGGTAGAGCAGCGCATTCGTAATGCGAAGGTCGTAGGTTCGACTCCTATTATCGGAACCATTAAAATCAAATTGt                                                                                                                       <  1:277032/140‑1 (MQ=255)                     gTCCACCGTGATTCACGTTCGTGAACATGTCCTTTCAGGGCCGATATAGCTCAGTTGGTAGAGCAGCGCATTCGTAATGCGAAGGTCGTAGGTTCGACTCCTATTATCGGAACCATTAAAATCAAATTGTTACGTAAGAt                                                                                                             >  2:247786/1‑140 (MQ=255)                                ttCACGTTCGTGAACATGTCCTTTCAGGGCCGATATAGCTCAGTTGGTAGAGCAGCGCATTCGTAATGCGAAGGTCGTAGGTTCGACTCCTATTATCGGAACCATTAAAATCAAATTGTTACGTAAGATCt                                                                                                           <  1:156411/131‑1 (MQ=255)                                ttCACGTTCGTGAACATGTCCTTTCAGGGCCGATATAGCTCAGTTGGTAGAGCAGCGCATTCGTAATGCGAAGGTCGTAGGTTCGACTCCTATTATCGGAACCATTAAAATCAAATTGTTACGTAAGATCt                                                                                                           >  2:156411/1‑131 (MQ=255)                                ttCACGTTCGTGAACATGTCCTTTCAGGGCCGATATAGCTCAGTTGGTAGAGCAGCGCATTCGTAATGCGAAGGTCGTAGGTTCGACTCCTATTATCGGAACCATTAAAATCAAATTGTTACGTAAGATCTTATCATtct                                                                                                  >  1:283108/1‑140 (MQ=255)                                      ttCGTGAACATGTCCTTTCAGGGCCGATATAGCTCAGTTGGTAGAGCAGCGCATTCGTAATGCGAAGGTCGTAGGTTCGACTCCTATTATCGGAACCATTAAAATCAAATTGTTACGTAAGATCTTATCATTCTCccacc                                                                                            <  2:329416/140‑1 (MQ=255)                                                 gTCCTTTCAGGGCCGATATAGCTCAGTTGGTAGAGCAGCGCATTCGTAATGCGAAGGTCGTAGGTTCGACTCCTATTATCGGAACCATTAAAATCAAATTGTTACGTAAGATCTTATCATTCTCCCACCAAAAAATTATc                                                                                 >  2:284745/1‑140 (MQ=255)                                                  tCCTTTCAGGGCCGATATAGCTCAGTTGGTAGAGCAGCGCATTCGTAATGCGAAGGTCGTAGGTTCGACTCCTATTATCGGAACCATTAAAATCAAATTGTTACGTAAGATCTTATCATTCTCCCACCAAAAAATTATCt                                                                                <  2:283108/140‑1 (MQ=255)                                                  tCCTTTCAGGGCCGATATAGCTCAGTTGGTAGAGCAGCGCATTCGTAATGCGAAGGTCGTAGGTTCGACTCCTATTATCGGAACCATTAAAATCAAATTGTTACGTAAGATCTTATCATTCTCCCACCAAAAAATTATCt                                                                                <  1:284745/140‑1 (MQ=255)                                                        cAGGGCCGATATAGCTCAGTTGGTAGAGCAGCGCATTCGTAATGCGAAGGTCGTAGGTTCGACTCCTATTATCGGAACCATTAAAATCAAATTGTTACGTAAGATCTTATCATTCTCCCACCAAAAAATTATCTTAATGt                                                                          <  1:257062/140‑1 (MQ=255)                                                             ccGATATAGCTCAGTTGGTAGAGCAGCGCATTCGTAATGCGAAGGTCGTAGGTTCGACTCCTATTATCGGAACCATTAAAATCAAATTGTTACGTAAGATCTTATCATTCTCCCACCAAAAAATTATCTTAATGTAACAg                                                                     <  1:219837/140‑1 (MQ=255)                                                              cGATATAGCTCAGTTGGTAGAGCAGCGCATTCGTAATGCGAAGGTCGTCGGTTCGCCTCCTATTATCGGAGCCATTAAAATCAAATTGTTACGTAAGATGTTATCATTCTCCCACCAAAAAATTATCTTAATGTAACAGc                                                                    <  2:269189/140‑1 (MQ=38)                                                              cGATATAGCTCAGTTGGTAGAGCAGCGCATTCGTAATGCGAAGGTCGTAGGTTCGACTCCTATTATCGGAACCATTAAAATCAAATTGTTACGTAAGATCTTATCATTCTCCCACCAAAAAATTATCTTAATGTAACAGc                                                                    <  1:323713/140‑1 (MQ=255)                                                                  ataGCTCAGTTGGTAGAGCAGCGCATTCGTAATGCGAAGGTCGTAGGTTCGACTCCTATTATCGGAACCATTAAAATCAAATTGTTACGTAAGATCTTATCATTCTCCCACCAAAAAATTATCTTAATGTAACAGCTGgt                                                                <  2:10607/140‑1 (MQ=255)                                                                                 gagCAGCGCATTCGTAATGCGAAGGTCGTAGGTTCGACTCCTATTATCGGAACCATTAAAATCAAATTGTTACGTAAGATCTTATCATTCTCCCACCAAAAAATTATCTTAATGTAACAGCTGGTGTAAGTAAATTCTAt                                                 <  1:103364/140‑1 (MQ=255)                                                                                     agcGCATTCGTAATGCGAAGGTCGTAGGTTCGACTCCTATTATCGGAACCATTAAAATCAAATTGTTACGTAAGATCTTATCATTCTCCCACCAAAAAATTATCTTAATGTAACAGCTGGTGTAAGTAAATTCTATCAAc                                             >  1:58936/1‑140 (MQ=255)                                                                                     agcGCATTCGTAATGCGAAGGTCGTAGGTTCGACTCCTATTATCGGAACCATTAAAATCAAATTGTTACGTAAGATCTTATCATTCTCCCACCAAAAAATTATCTTAATGTAACAGCTGGTGTAAGTAAATTCTATCAAc                                             >  1:115730/1‑140 (MQ=255)                                                                                       cgcATTCGTAATGCGAAGGTCGTAGGTTCGACTCCTATTATCGGAACCATTAAAATCAAATTGTTACGTAAGATCTTATCATTCTCCCACCAAAAAATTATCTTAATGTAACAGCTGGTGTAAGTAAATTCTATCAACGa                                           >  1:291547/1‑140 (MQ=255)                                                                                           ttCGTAATGCGAAGGTCGTAGGTTCGACTCCTATTATCGGAACCATTAAAATCAAATTGTTACGTAAGATCTTATCATTCTCCCACCAAAAAATTATCTTAATGTAACAGCTGGTGTAAGTAAATTCTATCAACGAAGAt                                       <  2:291547/140‑1 (MQ=255)                                                                                             cGTAATGCGAAGGTCGTAGGTTCGACTCCTATTATCGGAACCATTAAAATCAAATTGTTACGTAAGATCTTATCATTCTCCCACCAAAAAATTATCTTAATGTAACAGCTGGTGTAAGTAAATTCTATCAACGAAGATCa                                     <  1:8717/140‑1 (MQ=255)                                                                                                            gTAGGTTCGACTCCTATTATCGGAACCATTAAAATCAAATTGTTACGTAAGATCTTATCATTCTCCCACCAAAAAATTATCTTAATGTAACAGCTGGTGTAAGTAAATTCTATCAACGAAGATCAATCTTATCTACTGAc                      >  1:303115/1‑140 (MQ=255)                                                                                                                 ttCGACTCCTATTATCGGAACCATTAAAATCAAATTGTTACGTAAGATCTTATCATTCTCCCACCAAAAAATTATCTTAATGTAACAGCTGGTGTAAGTAAATTCTATCAACGAAGATCAATCTTATCTACTGACCaaaa                 <  1:123263/140‑1 (MQ=255)                                                                                                                    gACTCCTATTATCGGAACCATTAAAATCAAATTGTTACGTAAGATCTTATCATTCTCCCACCAAAAAATTATCTTAATGTAACAGCTGGTGTAAGTAAATTCTATCAACGAAGATCAATCTTATCTACTGACCAAAAAgg              <  1:54253/140‑1 (MQ=255)                                                                                                                    gACTCCTATTATCGGAACCATTAAAATCAAATTGTTACGTAAGATCTTATCATTCTCCCACCAAAAAATTATCTTAATGTAACAGCTGGTGTAAGTAAATTCTATCAACGAAGATCAATCTTATCTACTGACCAAAAAgg              <  2:303115/140‑1 (MQ=255)                                                                                                                           attatCGGAACCATTAAAATCAAATTGTTACGTAAGATCTTATCATTCTCCCACCAAAAAATTATCTTAATGTAACAGCTGGTGTAAGTAAATTCTATCAACGAAGATCAATCTTATCTACTGACCAAAAAGGCCTGATa       <  2:1567/140‑1 (MQ=255)                                                                                                                                cGGAACCATTAAAATCAAATTGTTACGTAAGATCTTATCATTCTCCCACCAAAAAATTATCTTAATGTAACAGCTGGTGTAAGTAAATTCTATCAACGAAGATCAATCTTATCTACTGACCAAAAAGGCCTGATAGGGCt  <  1:185595/140‑1 (MQ=255)                                                                                                                                   |                                                                                                                                         GCCCGGTTAGTTTTAACCTTGTCCACCGTGATTCACGTTCGTGAACATGTCCTTTCAGGGCCGATATAGCTCAGTTGGTAGAGCAGCGCATTCGTAATGCGAAGGTCGTAGGTTCGACTCCTATTATCGGCACCATTAAAATCAAATTGTTACGTAAGATCTTATCATTCTCCCACCAAAAAATTATCTTAATGTAACAGCTGGTGTAAGTAAATTCTATCAACGAAGATCAATCTTATCTACTGACCAAAAAGGCCTGATAGGGCT  >  NC_000913/262812‑263078

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑