Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	753,026	T→C	intergenic (‑231/+159)	ybgD ← / ← gltA	putative fimbrial protein YbgD/citrate synthase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	753,026	0	T	C	100.0%	46.1 / NA	14	intergenic (‑231/+159)	ybgD/gltA	putative fimbrial protein YbgD/citrate synthase
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base C (9/5);  total (9/5)

TTAGTTTTATTAAATGCAAAACTAAATTATTGGTATCATGAATTTGTTGTATGATGAATAAAATATAGGGGGGTATAGATAGACGTCATTTTCATAGGGTTATAAATGCGACTACCATGAAGTTTTTAATTGAAAGTATTGGGTTGCTGATAATTTGAGCTGTTCTATTCTTTTTAAATATCTATATAGGTCTGTTAATGGATTTTATTTTTACAATTTTTTGTGTTTAGGCATATAAAAATCAACCCGCCATATGAACGGCGGGTTAAAAT  >  NC_000913/752890‑753161                                                                                                                                         |                                                                                                                                        atAGTTTTATTAAATGCAAAACTAAATTATTGGAATAATGAATTTGTTGTATGATGAGTAAACTATAGGGGGGTATAGATAGACGTCATTTTCATAGGGTTATAAATGCGACTACCATGAAGTTTTTAATTGAAAGCAtt                                                                                                                                      >  2:153559/2‑140 (MQ=255)    gTTTTATTAAATGCAAAACTAAATTATTGGTATCATGAATTTGTTGTATGATGAATAAAATATAGGGGGGTATAGATAGACGTCATTTTCATAGGGTTATAAATGCGACTACCATGAAGTTTTTAATTGAAAGCATTggg                                                                                                                                   >  1:270636/1‑140 (MQ=255)               tGCAAAACTAAATTATTGGTATCATGAATTTGTTGTATGATGAATAAAATATAGGGGGGTATAGATAGACGTCATTTTCATAGGGTTATAAATGCGACTACCATGAAGTTTTTAATTGAAAGCATTGGGTTGCTGATAAt                                                                                                                        >  1:263409/1‑140 (MQ=255)               tGCAAAACTAAATTATTGGTATCATGAATTTGTTGTATGATGAATAAAATATAGGGGGGTATAGATAGACGTCATTTTCATAGGGTTATAAATGCGACTACCATGAAGTTTTTAATTGAAAGCATTGGGTTGCTGATAAt                                                                                                                        >  2:243996/1‑140 (MQ=255)                    aaCTAAATTATTGGTATCATGAATTTGTTGTATGATGAATAAAATATAGGGGGGTATAGATAGACGTCATTTTCATAGGGTTATAAATGCGACTACCATGAAGTTTTTAATTGAAAGCATTGGGTTGCTGATAATTTGAg                                                                                                                   <  1:243996/140‑1 (MQ=255)                    aaCTAAATTATTGGTATCATGAATTTGTTGTATGATGAATAAAATATAGGGGGGTATAGATAGACGTCATTTTCATAGGGTTATAAATGCGACTACCATGAAGTTTTTAATTGAAAGCATTGGGTTGCTGATAATTTGAg                                                                                                                   <  1:296193/140‑1 (MQ=255)                    aaCTAAATTATTGGTATCATGAATTTGTTGTATGATGAATAAAATATAGGGGGGTATAGATAGACGTCATTTTCATAGGGTTATAAATGCGACTACCATGAAGTTTTTAATTGAAAGCATTGGGTTGCTGATAATTTGAg                                                                                                                   <  2:313831/140‑1 (MQ=255)                              ttGGTATCATGAATTTGTTGTATGATGAATAAAATATAGGGGGGTATAGATAGACGTCATTTTCATAGGGTTATAAATGCGACTACCATGAAGTTTTTAATTGAAAGCATTGGGTTGCTGATAATTTGAGCTGTTCTAtt                                                                                                         <  1:96268/140‑1 (MQ=255)                                      aTGAATTTGTTGTATGATGAATAAAATATAGGGGGGTATAGATAGACGTCATTTTCATAGGGTTATAAATGCGACTACCATGAAGTTTTTAATTGAAAGCATTGGGTTGCTGATAATTTGAGCTGTTCTATTCTTTTTaa                                                                                                 >  1:177509/1‑140 (MQ=255)                                                tgtATGATGAATAAAATATAGGGGGGTATAGATAGACGTCATTTTCATAGGGTTATAAATGCGACTACCATGAAGTTTTTAATTGAAAGCATTGGGTTGCTGATAATTTGAGCTGTTCTATTCTTTTTAAATATCtatat                                                                                       >  1:32835/1‑140 (MQ=255)                                                  tATGATGAATAAAATATAGGGGGGTATAGATAGACGTCATTTTCATAGGGTTATAAATGCGACTACCATGAAGTTTTTAATTGAAAGCATTGGGTTGCTGATAATTTGAGCTGTTCTATTCTTTTTAAATATCTATATAg                                                                                     <  2:267507/140‑1 (MQ=255)                                                                                   aCGTCATTTTCATAGGGTTATAAATGCGACTACCATGAAGTTTTTAATTGAAAGCATTGGGTTGCTGATAATTTGAGCTGTTCTATTCTTTTTAAATATCTATATAGGTCTGTTAATGGATTTTATTTTTACAAtttttt                                                    >  1:16582/1‑140 (MQ=255)                                                                                       cATTTTCATAGGGTTATAAATGCGACTACCATGAAGTTTTTAATTGAAAGCATTGGGTTGCTGATAATTTGAGCTGTTCTATTCTTTTTAAATATCTATATAGGTCTGTTAATGGATTTTATTTTTACAATTTTTTgtgt                                                >  2:103035/1‑140 (MQ=255)                                                                                                            gCGACTACCATAAATTTTTTAATTAAAAGCTTTGGGTTGCTGATAATTTGAGTTTTTTTTTTTTTTTTAAATATCTATATAGGTCTGTTAATGGATTTTATTTTTACATTTTTTTGTTTTTAGGAATAAAAAAATCAccc                           <  2:165804/140‑1 (MQ=255)                                                                                                                      tGAAGTTTTTAATTGAAAGCATTGGGTTGCTGATAATTTGAGCTGTTCTATTCTTTTTAAATATCTATATAGGTCTGTTAATGGATTTTATTTTTACAATTTTTTGTGTTTAGGCATATAAAAATCAACCCGCCATATGa                 >  1:204661/1‑140 (MQ=255)                                                                                                                                     aaaGCATTGGGTTGCTGATAATTTGAGCTGTTATATTCTTTTTAAATATCTATATAGGTCTGTTAATGGATTTTATTTTTACAATTTTTTGTGTTTAGGCATATAAAAATCAACCCGCCATATGAACGGCGGGTTTAAat  >  2:234616/1‑140 (MQ=255)                                                                                                                                         |                                                                                                                                        TTAGTTTTATTAAATGCAAAACTAAATTATTGGTATCATGAATTTGTTGTATGATGAATAAAATATAGGGGGGTATAGATAGACGTCATTTTCATAGGGTTATAAATGCGACTACCATGAAGTTTTTAATTGAAAGTATTGGGTTGCTGATAATTTGAGCTGTTCTATTCTTTTTAAATATCTATATAGGTCTGTTAATGGATTTTATTTTTACAATTTTTTGTGTTTAGGCATATAAAAATCAACCCGCCATATGAACGGCGGGTTAAAAT  >  NC_000913/752890‑753161

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 31 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑