Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	1,690,971	C→A	L374M (CTG→ATG)	ydgA →	conserved protein YdgA

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,690,971	0	C	A	100.0%	69.4 / NA	21	L374M (CTG→ATG)	ydgA	conserved protein YdgA
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base A (11/10);  total (11/10)

CCAACAACCCCGAACTTTATCAGGAGAAAGTGACGGAAGCCTTCTTTAGCGCCCTGCCGCTGATGCTGAAAGGCGATCCGGTGATTACTATCGCGCCGCTAAGCTGGAAAAACAGTCAGGGTGAAAGTGCCCTGAATCTGTCGCTGTTCCTGAAAGATCCGGCAACGACTAAAGAAGCGCCGCAAACGCTGGCGCAGGAAGTAGATCGTTCGGTTAAATCTCTGGATGCGAAACTGACCATTCCGGTGGATATGGCAACTGAGTTTATGACTCA  >  NC_000913/1690834‑1691107                                                                                                                                          |                                                                                                                                         ccAACAACCCCGAACTTTATCAGGAGAAAGTGACGGAAGCCTTCTTTAGCGCCCTGCCGCTGATGCTGAAAGGCGATCCGGTGATTACTATCGCGCCGCTAAGCTGGAAAAACAGTCAGGGTGAAAGTGCCCTGAATATg                                                                                                                                        >  2:112161/1‑140 (MQ=255)    acaacCCCGAACTTTATCAGGAGAAAGTGACGGAAGCCTTCTTTAGCGCCCTGCCGCTGATGCTGAAAGGCGATCCGGTGATTACTATCGCGCCGCTAAGCTGGAAAAACAGTCAGGGTGAAAGTGCCCTGAATATGTCg                                                                                                                                     <  2:6056/140‑1 (MQ=255)                   aTCAGGAGAAAGTGACGGAAGCCTTCTTTAGCGCCCTGCCGCTGATGCTGAAAGGCGATCCGGTGATTACTATCGCGCCGCTAAGCTGGAAAAACAGTCAGGGTGAAAGTGCCCTGAATATGTCGCTGTTCCTGAAAGAt                                                                                                                      >  2:181172/1‑140 (MQ=255)                         agaAAGTGACGGAAGCCTTCTTTAGCGCCCTGCCGCTGATGCTGAAAGGCGATCCGGTGATTACTATCGCGCCGCTAAGCTGGAAAAACAGTCAGGGTGAAAGTGCCCTGAATATGTCGCTGTTCCTGAAAGATCCGGCa                                                                                                                <  2:121054/140‑1 (MQ=255)                                                       tGCCGCTGATGCTGAAAGGCGATCCGGTGATTACTATCGCGCCGCTAAGCTGGAAAAACAGTCAGGGTGAAAGTGCCCTGAATATGTCGCTGTTCCTGAAAGATCCGGCAACGACTAAAGAAGCGCCGCAAACGCTGgcg                                                                                  >  1:259185/1‑140 (MQ=255)                                                       tGCCGCTGATGCTGAAAGGCGATCCGGTGATTACTATCGCGCCGCTAAGCTGGAAAAACAGTCAGGGTGAAAGTGCCCTGAATATGTCGCTGTTCCTGAAAGATCCGGCAACGACTAAAGAAGCGCCGCAAACGCTGgcg                                                                                  <  2:295435/140‑1 (MQ=255)                                                        gCCGCTGATGCTGAAAGGCGATCCGGTGATTACTATCGCGCCGCTAAGCTGGAAAAACAGTCAGGGTGAAAGTGCCCTGAATATGTCGCTGTTCCTGAAAGATCCGGCAACGACTAAAGAAGCGCCGCAAACGCTGgcgc                                                                                 <  2:107052/140‑1 (MQ=255)                                                        gCCGCTGATGCTGAAAGGCGATCCGGTGATTACTATCGCGCCGCTAAGCTGGAAAAACAGTCAGGGTGAAAGTGCCCTGAATATGTCGCTGTTCCTGAAAGATCCGGCAACGACTAAAGAAGCGCCGCAAACGCTGgcgc                                                                                 <  2:173299/140‑1 (MQ=255)                                                                   tGAAAGGCGATCCGGTGATTACTATCGCGCCGCTAAGCTGGAAAAACAGTCAGGGTGAAAGTGCCCTGAATATGTCGCTGTTCCTGAAAGATCCGGCAACGACTAAAGAAGCGCCGCAAACGCTGGCGCAGGAAGTAGAt                                                                      >  2:9239/1‑140 (MQ=255)                                                                          cGATCCGGTGATTACTATCGCGCCGCTAAGCTGGAAAAACAGTCAGGGTGAAAGTGCCCTGAATATGTCGCTGTTCCTGAAAGATCCGGCAACGACTAAAGAAGCGCCGCAAACGCTGGCGCAGGAAGTAGATCGTTCgg                                                               <  1:39738/140‑1 (MQ=255)                                                                            aTCCGGTGATTACTATCGCGCCGCTAAGCTGGAAAAACAGTCAGGGTGAAAGTGCCCTGAATATGTCGCTGTTCCTGAAAGATCCGGCAACGACTAAAGAAGCGCCGCAAACGCTGGCGCAGGAAGTAGATCGTTCGGtt                                                             >  1:46186/1‑140 (MQ=255)                                                                                ggTGATTACTATCGCGCCGCTAAGCTGGAAAAACAGTCAGGGTGAAAGTGCCCTGAATATGTCGCTGTTCCTGAAAGATCCGGCAACGACTAAAGAAGCGCCGCAAACGCTGGCGCAGGAAGTAGATCGTTCGGTTAAAt                                                         <  2:61727/140‑1 (MQ=255)                                                                                           tCGCGCCGCTAAGCTGGAAAAACAGTCAGGGTGAAAGTGCCCTGAATATGTCGCTGTTCCTGAAAGATCCGGCAACGACTAAAGAAGCGCCGCAAACGCTGGCGCAGGAAGTAGATCGTTCGGTTAAATCTCTGGATGCg                                              >  2:278169/1‑140 (MQ=255)                                                                                              cgccgcTAAGCTGGAAAAACAGTCAGGGTGAAAGTGCCCTGAATATGTCGCTGTTCCTGAAAGATCCGGCAACGACTAAAGAAGCGCCGCAAACGCTGGCGCAGGAAGTAGATCGTTCGGTTAAATCTCTGGATGCGaaa                                           >  1:119496/1‑140 (MQ=255)                                                                                              cgccgcTAAGCTGGAAAAACAGTCAGGGTGAAAGTGCCCTGAATATGTCGCTGTTCCTGAAAGATCCGGCAACGACTAAAGAAGCGCCGCAAACGCTGGCGCAGGAAGTAGATCGTTCGGTTAAATCTCTGGATGCGaaa                                           >  2:260305/1‑140 (MQ=255)                                                                                                   cTAAGCTGGAAAAACAGTCAGGGTGAAAGTGCCCTGAATATGTCGCTGTTCCTGAAAGATCCGGCAACGACTAAAGAAGCGCCGCAAACGCTGGCGCAGGAAGTAGATCGTTCGGTTAAATCTCTGGATGCGAAACTGAc                                      <  2:120512/140‑1 (MQ=255)                                                                                                   cTAAGCTGGAAAAACAGTCAGGGTGAAAGTGCCCTGAATATGTCGCTGTTCCTGAAAGATCCGGCAACGACTAAAGAAGCGCCGCAAACGCTGGCGCAGGAAGTAGATCGTTCGGTTAAATCTCTGGATGCGAAACTGAc                                      <  2:119496/140‑1 (MQ=255)                                                                                                   cTAAGCTGGAAAAACAGTCAGGGTGAAAGTGCCCTGAATATGTCGCTGTTCCTGAAAGATCCGGCAACGACTAAAGAAGCGCCGCAAACGCTGGCGCAGGAAGTAGATCGTTCGGTTAAATCTCTGGATGCGAAACTGAc                                      >  1:328701/1‑140 (MQ=255)                                                                                                     aaGCTGGAAAAACAGTCAGGGTGAAAGTGCCCTGAATATGTCGCTGTTCCTGAAAGATCCGGCAACGACTAAAGAAGCGCCGCAAACGCTGGCGCAGGAAGTAGATCGTTCGGTTAAATCTCTGGATGCGAAACTGACCa                                    <  1:112161/140‑1 (MQ=255)                                                                                                                                tGCCCTGAATATGTCGCTGTTCCTGAAAGATCCGGCAACGACTAAAGAAGCGCCGCAAACGCTGGCGCAGGAAGTAGATCGTTCGGTTAAATCTCTGGATGCGAAACTGACCATTCCGGTGGATATGGCAACTGAGTTTa         >  2:126018/1‑140 (MQ=255)                                                                                                                                  cccTGCATATGTCGCTGGTCCTGAAACATCCGGCCACGACTAAAGAACCGCCGCAAACGCTGGCGCAGGAAGTAGATCGTTCGGTTAAATCTCTGGATGCGAAACTGACCATTCCGGTGGATATGACAACTGAGTTTATg       >  2:78298/1‑140 (MQ=255)                                                                                                                                       aaTATGTCGCTGTTCCTGAAAGATCCGGCAACGACTAAAGAAGCGCCGCAAACGCTGGCGCAGGAAGTAGATCGTTCGGTTAAATCTCTGGATGCGAAACTGACCATTCCGGTGGATATGGCAACTGAGTTTATGACTCa  >  2:4237/1‑140 (MQ=255)                                                                                                                                          |                                                                                                                                         CCAACAACCCCGAACTTTATCAGGAGAAAGTGACGGAAGCCTTCTTTAGCGCCCTGCCGCTGATGCTGAAAGGCGATCCGGTGATTACTATCGCGCCGCTAAGCTGGAAAAACAGTCAGGGTGAAAGTGCCCTGAATCTGTCGCTGTTCCTGAAAGATCCGGCAACGACTAAAGAAGCGCCGCAAACGCTGGCGCAGGAAGTAGATCGTTCGGTTAAATCTCTGGATGCGAAACTGACCATTCCGGTGGATATGGCAACTGAGTTTATGACTCA  >  NC_000913/1690834‑1691107

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑