Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_000913501,5640AC33.3% 32.1 / 15.7 24*559E (TAA→GAA) ybaLputative transporter YbaL
Reads supporting (aligned to +/- strand):  ref base A (15/1);  new base C (0/8);  total (15/9)
Fisher's exact test for biased strand distribution p-value = 1.22e-05
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00
Rejected as consensus: Frequency below/above cutoff threshold.

TTATCGTCGGTTCGTAGGCCAGATAAGGCGTTCACGCCGCATCTGGCATTTGGCTCTCGATGCCTGATGCGACGCTGGCGCGTCTTATCATGCCTACATATTTTTCATATTTTACATCCGGCAACCACCGTTTACCCCGTCACCACCTCACCCGCCGGTGGCGTTTCCAGCAGTTCCAGCATGGTACGGGCGATTTCACGCTCGCCCATCACTACCTGATTCGCACCACGTTCGGTGATATACGCCACTTCATCGTCATA  >  NC_000913/501431‑501690
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ttATCGTCGGTTCGTAGGCCAGATAAGGCGTTCACGCCGCATCTGGCATTTGGCTCTCGATGCCTGATGCGACGCTGGCGCGTCTTATCATGCCTACATATTTTTCATATTTTACATCCGGCAACCCCCGTTTAcc                                                                                                                              <  1:686440/136‑1 (MQ=255)
ttATCGTCGGTTCGTAGGCCAGATAAGGCGTTCACGCCGCATCTGGCATTTGGCTCTCGATGCCTGATGCGACGCTGGCGCGTCTTATCATGCCTACATATTTTTCATATTTTACATCCGGCAACCACCGTTTAcc                                                                                                                              >  2:686440/1‑136 (MQ=255)
 tATCGTCGGTTCGTAGGCCAGATAAGGCGTTCACGCCGCATCTGGCATTTGGCTCTCGATGCCTGATGCGACGCTGGCGCGTCTTATCATGCCTACATATTTTTCATATTTTACATCCGGCAACCACCGTTTACCCCGTc                                                                                                                         >  1:511866/1‑140 (MQ=255)
   tcgtcgGTTCGTAGGCCAGATAAGGCGTTCACGCCGCATCTGGCATTTGGCTCTCGATGCCTGATGCGACGCTGGCGCGTCTTATCATGCCTACATATTTTTCATATTTTACATCCGGCAACCACCGTTTACCCCGTcac                                                                                                                       >  1:285007/1‑140 (MQ=255)
   tcgtcgGTTCGTAGGCCAGATAAGGCGTTCACGCCGCATCTGGCATTTGGCTCTCGATGCCTGATGCGACGCTGGCGCGTCTTATCATGCCTACATATTTTTCATATTTTACATCCGGCAACCACCGTTTACCCCGTcac                                                                                                                       >  1:655356/1‑140 (MQ=255)
          ttCGTAGGCCAGATAAGGCGTTCACGCCGCATCTGGCATTTGGCTCTCGATGCCTGATGCGACGCTGGCGCGTCTTATCATGCCTACATATTTTTCATATTTTACATCCGGCAACCACCGTTTACCCCGTCACCACCTCa                                                                                                                >  1:265055/1‑140 (MQ=255)
                              ttgtctccgcATCTGTCTTTTGGCACAAGATCACTTATCGTACGCTGGCCCTCTTTGTCATGCCTTCATTTTTTCTATATTTAACATCCGTCACCCACGTTTTACCCGATCACAACCTCCCCCGCCGGTGGCGTTTCcag                                                                                            <  2:323288/134‑1 (MQ=255)
                              ttCACGCCGCATCTGGCATTTGGCTCTCGATGCCTGATGCGACGCTGGCGCGTCTTATCATGCCTACATATTTTTCATGTTTTACATCCGGCAACCACCGTTTACCCCGTCACCACCTCACCCGCCGGTGGCGTTTCcag                                                                                            >  1:206553/1‑140 (MQ=255)
                                         tCTGGCATTTGGCTCGTGATGCTTGTTGCATCGCTGGCGCGTCTTATCTTTCCTACTTTTTTTTCATTTTTCCCTCCCGCCACCCTCCTTTTCCCCCTCCCCCCCCTCACCCGCCGGTGGCGTTTCCAGCAGTTCCAGCa                                                                                 <  2:290519/140‑1 (MQ=255)
                                                    gCTCTCGATGCCTGATGCGACGCTGGCGCGTCTTATCATGCCTACATATTTTTCATATTTTACATCCGGCAACCACCGTTTACCCCGTCACCACCTCACCCGCCGGTGGCGTTTCCAGCAGTTCCAGCATGGTACGGGCg                                                                      >  1:479774/1‑140 (MQ=255)
                                                       ctcGATGCCTGATGCGACGCTGGCGCGTCTTATCATGCCTACATATTTTTCATATTTTACATCCGGCAACCACCGTTTACCCCGTCACCACCTCACCCGCCGGTGGCGTTTCCAGCAGTTCCAGCATGGTACGGGCGAtt                                                                   >  1:201830/1‑140 (MQ=255)
                                                                 aaTGCGACGCGGGCCCGTCTTAACCTGCCTACATTTTTTTCATATTTTACCCCGGGCAACCCCCTTTTCCCCCGTCCCCCCCCCCCCCGCCGGTGGCGTTTCCAGCAGTTCCAGCATGGTACGGGCGATTTCACGCTCGc                                                         <  2:16804/139‑1 (MQ=255)
                                                                    gcgcCGCTGGCGCGTTTTATCATGCCTAAATTTTTTTCATTTTTTACATCCGCCACCCCCCGTTTCCCCCTTCCCCCCCTCCCCCGCCGGTGGCGTTTCCAGCAGTTCCAGCATGGTACGGGCGATTTCACGCTCGCCCa                                                      <  2:460525/140‑1 (MQ=255)
                                                                       ccGCTGGCGCGTTTTATCATGCCTACATTTTTTTCATATTTTACACCCGGCACCCACCTTTTCCCCCGCCCCCCCCTCCCCCGCCGGTGGCGTTTCCAGCAGTTCCAGCATGGTACGGGCGATTTCACGCTCGCCCATCa                                                   <  2:295607/139‑1 (MQ=255)
                                                                              cgctttttATCATGCCTACTTTTTTTTCATTTTTTACATCCGGCACCCCCCTTTTCCCCCGCCCCCCCCTCCCCCGCCGGTGGCGTTTCCAGCAGTTCCAGCATGGTACGGGCGATTTCACGCTCGCCCATCACTACCTg                                            <  2:552222/134‑1 (MQ=255)
                                                                              cgcgTCTTATCATGCCTACATATTTTTCATATTTTACATCCGGCAACCACCGTTTACCCCGTCACCACCTCACCCGCCGGTGGCGTTTCCAGCAGTTCCAGCATGGTACGGGCGATTTCACGCTCGCCCATCACTACCTg                                            >  1:184157/1‑140 (MQ=255)
                                                                                 tttttATCATCCCTACATATTTTTCATATTTTACACCCGCAACCCCCCGTTCCCCCCCTCCCCCCCCCCCCCGCCGGTGGCGTTTCCAGCAGTTCCAGCATGGTACGGGCGATTTCACGCTCGCCCATCACTACCTGAtt                                         <  2:453493/137‑1 (MQ=255)
                                                                                 gTCTTATCATGCCTACATATTTTTCATATTTTACATCCGGCAACCACCGTTTACCCCGTCACCACCTCACCCGCCGGTGGCGTTTCCAGCAGTTCCAGCATGGTACGGGCGATTTCACGCTCGCCCATCACTACCTGAtt                                         >  1:519186/1‑140 (MQ=255)
                                                                                    ttATCATGCCTACATATTTTTCATATTTTACATCCGGCAACCACCGTTTACCCCGTCACCACCTCACCCGCCGGTGGCGTTTCCAGCAGTTCCAGCATGGTACGGGCGATTTCACGCTCGCCCATCACTACCTGATTCGc                                      >  1:248558/1‑140 (MQ=255)
                                                                                       tCATCCCTACATTTTTTTCATTTTTTACACCCGGCACCCACCTTTTCCCCCTTCCCCCCCTCCCCCGCCGGTGGCGTTTCCAGCAGTTCCAGCATGGTACGGGCGATTTCACGCTCGCCCATCACTACCTGATTCGcacc                                   <  2:285689/140‑1 (MQ=255)
                                                                                          tcccTACTTTTTTTTCATTTTTTACACCCGGCACCCCCCTTTTCCCCCGTCCCCCCCTCCCCCGCCGGTGGCGTTTCCAGCAGTTCCAGCATGGTACGGGCGATTTCACGCTCGCCCATCACTACCTGATTCGCACCACg                                <  2:659773/138‑1 (MQ=255)
                                                                                          tGCCTACATTTTTTTCATATTTTACATCCGGCACCCCCCTTTTCCCCCGTCCCCCCCTCCCCCGCCGGTGGCGTTTCCAGCAGTTCCAGCATGGTACGGGCGATTTCACGCTCGCCCATCACTACCTGATTCGCACCACg                                <  1:61979/140‑1 (MQ=255)
                                                                                            ccTACATTTTTTTCATTTTTTCCACCCGGCAACCCCCTTTTCCCCCGTCCCCCCCCCCCCCGCCGGTGGCGTTTCCAGCAGTTCCAGCATGGTACGGGCGATTTCACGCTCGCCCATCACTACCTGATTCGCACCACGtt                              <  2:265055/140‑1 (MQ=255)
                                                                                             cTACTTTTTTTTCATATTTTCCATCCGGCAACCCCCGTTTCCCCCGCCCCCCCCCCCCCCGCCGGTGGCGTTTCCAGCAGTTCCAGCATGGTACGGGCGATTTCACGCTCGCCCATCACTACCTGATTCGCACCACGTTc                             <  2:93274/140‑1 (MQ=255)
                                                                                               acttttttttCATTTTTTCCACCCGCCACCCCCCTTTTCCCCCGTCCCCCCCCCCCCCGCCGGTGGCGTTTCCAGCAGTTCCAGCATGGTACGGGCGATTTCACGCTCGCCCATCACTACCTGATTCGCACCACGTTCgg                           <  2:362226/135‑1 (MQ=255)
                                                                                               aCATTTTTTTCATATTTTACACCCGGCAACCCCCGTTTCCCCCGTCCCCCCCTCCCCCGCCGGTGGCGTTTCCAGCAGTTCCAGCATGGTACGGGCGATTTCACGCTCGCCCATCACTACCTGATTCGCACCACGTTCgg                           <  1:552759/140‑1 (MQ=255)
                                                                                                 atttttttCATATTTTACATCCGGCACCCACCTTTTCCCCCGTCCCCCCCTCCCCCGCCGGTGGCGTTTCCAGCAGTTCCAGCATGGTACGGGCGATTTCACGCTCGCCCATCACTACCTGATTCGCACCACGTTCGGTg                         <  1:560740/137‑1 (MQ=255)
                                                                                                     ttttCATACTTTACATCCGGCAACCACCGTTTACCCCGTCACCACCTCACCCGCCGGTGGCGTTTCCAGCAGTTCCAGCATGGTACGGGCGATTTCACGCTCGCCCATCACTACCTGATTCGCACCACGTTCGGTGatat                     >  1:240415/1‑140 (MQ=255)
                                                                                                          atatTTTACATCCGGCAACCACCGTTTACCCCGTCACCACCTCACCCGCCGGTGGCGTTTCCAGCAGTTCCAGCATGGTACGGGCGATTTCACGCTCGCCCATCACTACCTGATTCGCACCACGTTCGGTGATATACGcc                >  1:138341/1‑140 (MQ=255)
                                                                                                                tACACCCGGCACCCCCCTTTTCCCCCCCCCCCCCCTCCCCCGCCGGTGGCGTTTCCAGCAGTTCCAGCATGGTACGGGCGATTTCACGCTCGCCCATCACTACCTGATTCGCACCACGTCCGGTGATATACGCCACTTCa          <  2:201830/140‑1 (MQ=255)
                                                                                                                tACACCCGGCACCCACCTTTTCCCCCGTCACCCCCCCCCCCGCCGGTGGCGTTTCCAGCAGTTCCAGCATGGTACGGGCGATTTCACGCTCGCCCATCACTACCTGATTCGCACCACGTTCGGTGATATACGCCACTTCa          <  2:655356/140‑1 (MQ=255)
                                                                                                                  cacccGGCACCCCCCTTTTCCCCCGTCCCCCCCTCCCCCGCCGGTGGCGTTTCCAGCAGTTCCAGCATGGTACGGGCGATTTCACGCTCGCCCATCACTACCTGATTCGCACCACGTTCGGTGATATACGCCACTTCATc        <  2:615671/137‑1 (MQ=255)
                                                                                                                       ggCAACCACCGTTTACCCCGTCACCACCTCACCCGCCGGTGGCGTTTCCAGCAGTTCCAGCATGGTACGGGCGATTTCACGCACGCCCATCACTACCTGATTCGCACCACGTTCGGTGATATACGCCACTTCATCGTCAt   >  1:455360/1‑140 (MQ=255)
                                                                                                                        gCAACCACCGTTTACCCCGTCACCACCTCACCCGCCGGTGGCGTTTCCAGCAGTTCCAGCATGGTACGGGCGATTTCACGCTCGCCCATCACTACCTGATTCGCACCACGTTCGGTGATATACGCCACTTCATCGTCATa  >  2:631747/1‑140 (MQ=255)
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TTATCGTCGGTTCGTAGGCCAGATAAGGCGTTCACGCCGCATCTGGCATTTGGCTCTCGATGCCTGATGCGACGCTGGCGCGTCTTATCATGCCTACATATTTTTCATATTTTACATCCGGCAACCACCGTTTACCCCGTCACCACCTCACCCGCCGGTGGCGTTTCCAGCAGTTCCAGCATGGTACGGGCGATTTCACGCTCGCCCATCACTACCTGATTCGCACCACGTTCGGTGATATACGCCACTTCATCGTCATA  >  NC_000913/501431‑501690

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 29 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑