Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	2,091,937	G→A	V281I (GTT→ATT)	hisD →	histidinal/histidinol dehydrogenase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	2,091,937	0	G	A	100.0%	105.2 / NA	31	V281I (GTT→ATT)	hisD	histidinal/histidinol dehydrogenase
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base A (18/13);  total (18/13)

CGTCGGAAGTGCTGGTGATTGCTGACAGCGGCGCTACGCCGGATTTCGTGGCTTCTGATTTGCTCTCTCAGGCTGAACACGGCCCGGACTCACAGGTGATTTTACTGACGCCCGCTGCTGATATGGCGCGTCGCGTTGCCGAGGCCGTCGAACGCCAACTGGCAGAACTGCCGCGTGCCGAAACCGCCCGCCAGGCACTGAACGCCAGCCGCCTGATCGTGACTAAAGATTTAGCGCAGTGCGTGGAGATCTCCAACCAGTACGGCCCGGA  >  NC_000913/2091803‑2092073                                                                                                                                       |                                                                                                                                         cGTCGGAAGTGCTGGTGATTGCTGACAGCGGCGCTACGCCGGATTTCGTGGCTTCTGATTTGCTCTCTCAGGCTGAACACGGCCCGGACTCACAGGTGATTTTACTGACGCCCGCTGCTGATATGGCGCGTCGCATTGcc                                                                                                                                     >  2:189221/1‑140 (MQ=255)                       tGACAGCGGCGCTACGCCGGATTTCGTGGCTTCTGATTTGCTCTCTCAGGCTGAACACGGCCCGGACTCACAGGTGATTTTACTGACGCCCGCTGCTGATATGGCGCGTCGCATTGCCGAGGCCGTCGAACGCCAACTgg                                                                                                               <  2:40321/140‑1 (MQ=255)                           aGCGGCGCTACGCCGGATTTCGTGGCTTCTGATTTGCTCTCTCAGGCTGAACACGGCCCGGACTCACAGGTGATTTTACTGACGCCCGCTGCTGATATGGCGCGTCGCATTGCCGAGGCCGTCGAACGCCAACTGGCAGa                                                                                                           >  2:128810/1‑140 (MQ=255)                            gcggcgCTACGCCGGATTTCGTGGCTTCTGATTTGCTCTCTCAGGCTGAACACGGCCCGGACTCACAGGTGATTTTACTGACGCCCGCTGCTGATATGGCGCGTCGCATTGCCGAGGCCGTCGAACGCCa                                                                                                                    >  2:243277/1‑130 (MQ=255)                            gcggcgCTACGCCGGATTTCGTGGCTTCTGATTTGCTCTCTCAGGCTGAACACGGCCCGGACTCACAGGTGATTTTACTGACGCCCGCTGCTGATATGGCGCGTCGCATTGCCGAGGCCGTCGAACGCCa                                                                                                                    <  1:243277/130‑1 (MQ=255)                             cggcgCTACGCCGGATTTCGTGGCTTCTGATTTGCTCTCTCAGGCTGAACACGGCCCGGACTCACAGGTGATTTTACTGACGCCCGCTGCTGATATGGCGCGTCGCATTGCCGAGGCCGTCGAACGCCAACTGGCAGAAc                                                                                                         >  1:70528/1‑140 (MQ=255)                                cgcTACGCCGGATTTCGTGGCTTCTGATTTGCTCTCTCAGGCTGAACACGGCCCGGACTCACAGGTGATTTTACTGACGCCCGCTGCTGATATGGCGCGTCGCATTGCCGAGGCCGTCGAACGCCAACTGGCAGAACTGc                                                                                                      >  2:361725/1‑140 (MQ=255)                                                gTGGCTTCTGATTTGCTCTCTCAGGCTGAACACGGCCCGGACTCACAGGTGATTTTACTGACGCCCGCTGCTGATATGGCGCGTCGCATTGCCGAGGCCGTCGAACGCCAACTGGCAGAACTGCCGCGTGCCGAAAccgc                                                                                      <  1:13189/140‑1 (MQ=255)                                                              gCTCTCTCAGGCTGAACACGGCCCGGACTCACAGGTGATTTTACTGACGCCCGCTGCTGATATGGCGCGTCGCATTGCCGAGGCCGTCGAACGCCAACTGGCAGAACTGCCGCGTGCCGAAACCGCCCGCCAGGCACTGa                                                                        >  1:233815/1‑140 (MQ=255)                                                              gCTCTCTCAGGCTGAACACGGCCCGGACTCACAGGTGATTTTACTGACGCCCGCTGCTGATATGGCGCGTCGCATTGCCGAGGCCGTCGAACGCCAACTGGCAGAACTGCCGCGTGCCGAAACCGCCCGCCAGGCACTGa                                                                        >  1:660873/1‑140 (MQ=255)                                                               ctctctCAGGCTGAACACGGCCCGGACTCACAGGTGATTTTACTGACGCCCGCTGCTGATATGGCGCGTCGCATTGCCGAGGCCGTCGAACGCCAACTGGCAGAACTGCCGCGTGCCGAAACCGCCCGCCAGGCACTGaa                                                                       <  1:361725/140‑1 (MQ=255)                                                                            aaCACGGCCCGGACTCACAGGTGATTTTACTGACGCCCGCTGCTGATATGGCGCGTCGCATTGCCGAGGCCGTCGAACGCCAACTGGCAGAACTGCCGCGTGCCGAAACCGCCCGCCAGGCACTGAACGCCAgccgc                                                             >  2:502918/1‑137 (MQ=255)                                                                            aaCACGGCCCGGACTCACAGGTGATTTTACTGACGCCCGCTGCTGATATGGCGCGTCGCATTGCCGAGGCCGTCGAACGCCAACTGGCAGAACTGCCGCGTGCCGAAACCGCCCGCCAGGCACTGAACGCCAgccgc                                                             <  1:502918/137‑1 (MQ=255)                                                                                   cccGGACTCACAGGTGATTTTACTGACGCCCGCTGCTGATATGGCGCGTCGCATTGCCGAGGCCGTCGAACGCCAACTGGCAGAACTGCCGCGTGCCGAAACCGCCCGCCAGGCACTGAACGCCAGCCGCCTGATCGTGa                                                   >  1:603123/1‑140 (MQ=255)                                                                                      ggACTCACAGGTGATTTTACTGACGCCCGCTGCTGATATGGCGCGTCGCATTGCCGAGGCCGTCGAACGCCAACTGGCAGAACTGCCGCGTGCCGAAACCGCCCGCCAGGCACTGAACGCCAGCCGCCTGATCGTGACTa                                                >  2:61843/1‑140 (MQ=255)                                                                                       gACTCACAGGTGATTTTACTGACGCCCGCTGCTGATATGGCGCGTCGCATTGCCGAGGCCGTCGAACGCCAACTGGCAGAACTGCCGCGTGCCGAAACCGCCCGCCAGGCACTGAACGCCAGCCGCCTGATCGTGACTaa                                               <  2:168153/140‑1 (MQ=255)                                                                                        aCTCACAGGTGATTTTACTGACGCCCGCTGCTGATATGGCGCGTCGCATTGCCGAGGCCGTCGAACGCCAACTGGCAGAACTGCCGCGTGCCGAAACCGCCCGCCAGGCACTGAACGCCAGCCGCCTGATCGTGACTaaa                                              >  1:376054/1‑140 (MQ=255)                                                                                            acaGGTGATTTTACTGACGCCCGCTGCTGATATGGCGCGTCGCATTGCCGAGGCCGTCGAACGCCAACTGGCAGAACTGCCGCGTGCCGAAACCGCCCGCCAGGCACTGAACGCCAGCCGCCTGATCGTGACTAAAGAtt                                          >  2:350480/1‑140 (MQ=255)                                                                                             caGGTGATTTTACTGACGCCCGCTGCTGATATGGCGCGTCACATTGCCGAGGCCGTCGAACGACAACTGGCAGAACTGCCGCGTGCCGAAACCGCCCGCCAGGCACTGAACGCCAGCCGCCTGATCGTGACTAAAGAttt                                         >  2:646039/1‑140 (MQ=255)                                                                                                gTGATTTTACTGACGCCCGCTGCTGATATGGCGCGTCGCATTGCCGAGGCCGTCGAACGCCAACTGGCAGAACTGCCGCGTGCCGAAACCGCCCGCCAGGCACTGAACGCCAGCCGCCTGATCGTGACTAAAGATTTAgc                                      >  2:636132/1‑140 (MQ=255)                                                                                                  gATTTTACTGACGCCCGCTGCTGATATGGCGCGTCGCATTGCCGAGGCCGTCGAACGCCAACTGGCAGAACTGCCGCGTGCCGAAACCGCCCGCCAGGCACTGAACGCCAGCCGCCTGATCGTGACTAAAGATTTAgcgc                                    <  1:189221/140‑1 (MQ=255)                                                                                                   aTTTTACTGACGCCCGCTGCTGATATGGCGCGTCGCATTGCCGAGGCCGTCGAACGCCAACTGGCAGAACTGCCGCGTGCCGAAACCGCCCGCCAGGCACTGAACGCCAGCCGCCTGATCGTGACTAAAGATTTAGCGCa                                   <  2:83364/140‑1 (MQ=255)                                                                                                   aTTTTACTGACGCCCGCTGCTGATATGGCGCGTCGCATTGCCGAGGCCGTCGAACGCCAACTGGCAGAACTGCCGCGTGCCGAAACCGCCCGCCAGGCACTGAACGCCAGCCGCCTGATCGTGACTAAAGATTTAGCGCa                                   <  2:660873/140‑1 (MQ=255)                                                                                                   aTTTTACTGACGCCCGCTGCTGATATGGCGCGTCGCATTGCCGAGGCCGTCGAACGCCAACTGGCAGAACTGCCGCGTGCCGAAACCGCCCGCCAGGCACTGAACGCCAGCCGCCTGATCGTGACTAAAGATTTAGCGCa                                   <  1:128810/140‑1 (MQ=255)                                                                                                   aTTTTACTGACGCCCGCTGCTGATATGGCGCGTCGCATTGCCGAGGCCGTCGAACGCCAACTGGCAGAACTGCCGCGTGCCGAAACCGCCCGCCAGGCACTGAACGCCAGCCGCCTGATCGTGACTAAAGATTTAGCGCa                                   <  2:148086/140‑1 (MQ=255)                                                                                                   aTTTTACTGACGCCCGCTGCTGATATGGCGCGTCGCATTGCCGAGGCCGTCGAACGCCAACTGGCAGAACTGCCGCGTGCCGAAACCGCCCGCCAGGCACTGAACGCCAGCCGCCTGATCGTGACTAAAGATTTAGCGCa                                   <  1:299595/140‑1 (MQ=255)                                                                                                            aCGCCCGCTGCTGATATGGCGCGTCGCATTGCCGAGGCCGTCGAACGCCAACTGGCAGAACTGCCGCGTGCCGAAACCGCCCGCCAGGCACTGAACGCCAGCCGCCTGATCGTGACTAAAGATTTAGCGCAGTGCGTGga                          <  2:70528/140‑1 (MQ=255)                                                                                                             cGCCCGCTGCTGATATGGCGCGTCGCATTGCCGAGGCCGTCGAACGCCAACTGGCAGAACTGCCGCGTGCCGAAACCGCCCGCCAGGCACTGAACGCCAGCCGCCTGATCGTGACTAAAGATTTAGCGCAGTGCGTGgag                         >  1:54652/1‑140 (MQ=255)                                                                                                              gCCCGCTGCTGATATGGCGCGTCGCATTGCCGAGGCCGTCGAACGCCAACTGGCAGAACTGCCGCGTGCCGAAACCGCCCGCCAGGCACTGAACGCCAGCCGCCTGATCGTGACTAAAGATTTAGCGCAGTGCGTGgaga                        >  2:420847/1‑140 (MQ=255)                                                                                                              gCCCGCTGCTGATATGGCGCGTCGCATTGCCGAGGCCGTCGAACGCCAACTGGCAGAACTGCCGCGTGCCGAAACCGCCCGCCAGGCACTGAACGCCAGCCGCCTGATCGTGACTAAAGATTTAGCGCAGTGCGTGgaga                        >  1:319759/1‑140 (MQ=255)                                                                                                                              gcgcGTCGCATTGCCGAGGCCGTCGAACGCCAACTGGCAGAACTGCCGCGTGCCGAAACCGCCCGCCAGGCACTGAACGCCAGCCGCCTGATCGTGACTAAAGATTTAGCGCAGTGCGTGGAGATCTCCAACCAGTACgg        >  1:187571/1‑140 (MQ=255)                                                                                                                                  gtcgcatTGCCGAGGCCGTCGAACGCCAACTGGCAGAACTGCCGCGTGCCGAAACCGCCCGCCAGGCACTGAACGCCAGCCGCCTGATCGTGACTAAAGATTTAGCGCAGTGCGTGGAGATCTCCAACCAGTACGGCCCg    <  1:420847/140‑1 (MQ=255)                                                                                                                                   tcgcatTGCCGAGGCCGTCGAACGCCAACTGGCAGAACTGCCGCGTGCCGAAACCGCCCGCCAGGCACTGAACGCCAGCCGCCTGATCGTGACTAAAGATTTAGCGCAGTGCGTGGAGATCTCCAACCAGTACGGCCCgg   >  2:187585/1‑140 (MQ=255)                                                                                                                                   tcgcatTGCCGAGGCCGTCGAACGCCAACTGGCAGAACTGCCGCGTGCCGAAACCGCCCGCCAGGCACTGAACGCCAGCCGCCTGATCGTGACTAAAGATTTAGCGCAGTGCGTGGAGATCTCCAACCAGTACGGCCCgg   <  2:501335/140‑1 (MQ=255)                                                                                                                                    cgcatTGCCGAGGCCGTCGAACGCCAACTGGCAGAACTGCCGCGTGCCGAAACCGCCCGCCAGGCACTGAACGCCAGCCGCCTGATCGTGACTAAAGATTTAGCGCAGTGCGTGGAGATCTCCAACCAGTACGGCCCGGa  >  2:148759/1‑140 (MQ=255)                                                                                                                                       |                                                                                                                                         CGTCGGAAGTGCTGGTGATTGCTGACAGCGGCGCTACGCCGGATTTCGTGGCTTCTGATTTGCTCTCTCAGGCTGAACACGGCCCGGACTCACAGGTGATTTTACTGACGCCCGCTGCTGATATGGCGCGTCGCGTTGCCGAGGCCGTCGAACGCCAACTGGCAGAACTGCCGCGTGCCGAAACCGCCCGCCAGGCACTGAACGCCAGCCGCCTGATCGTGACTAAAGATTTAGCGCAGTGCGTGGAGATCTCCAACCAGTACGGCCCGGA  >  NC_000913/2091803‑2092073

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 23 ≤ ATCG/ATCG < 30 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑