| Predicted mutation | ||||||
|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | annotation | gene | description |
| RA | NC_000913 | 2,274,149 | C→A | L596M (CTG→ATG) | yejA → | putative oligopeptide ABC transporter periplasmic binding protein |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_000913 | 2,274,149 | 0 | C | A | 95.6% | 144.4 / ‑1.8 | 45 | L596M (CTG→ATG) | yejA | putative oligopeptide ABC transporter periplasmic binding protein |
| Reads supporting (aligned to +/- strand): ref base C (0/0); major base A (25/18); minor base G (1/1); total (26/19) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.04e-01 | |||||||||||
TGCTGCCAATGTGGTACATGGCGGAAGACCGTCTCGCCTGGTGGGATAAATTCTCCCAGCCGGCCGTGCGCCCCATCTATAGCCTCGGTATCGATACCTGGTGGTATGACGTCAATAAAGCGGCCAAACTGCCGTCCGCCAGCAAACAGGGAGAGTAGATGGGCGCTTACCTGATTCGCCGTCTGTTGCTGGTGATCCCAACATTATGGGCGATTATCACCATC‑AACTTTTTCATCGTGCAAATTGCGCCTGGCGGTCCGG > NC_000913/2274021‑2274281 | tgctgcCAATGTGGTACATGGCGGAAGACCGTCTCGCCTGGTGGGATAAATTCTCCCAGCCGGCCGTGCGCCCCATCTATAGCCTCGGTATCGATACCTGGTGGTATGACGTCAATAAAGCGGCCAAAATGCCGTCCGcc > 1:118203/1‑140 (MQ=255)tgctgcCAATGTGGTACATGGCGGAAGACCGTCTCGCCTGGTGGGATAAATTCTCCCAGCCGGCCGTGCGCCCCATCTATAGCCTCGGTATCGATACCTGGTGGTATGACGTCAATAAAGCGGCCAAAATGCCGTCCGcc > 1:170295/1‑140 (MQ=255)tgctgcCAATGTGGTACATGGCGGAAGACCGTCTCGCCTGGTGGGATAAATTCTCCCAGCCGGCCGTGCGCCCCATCTATAGCCTCGGTATCGATACCCGGTGGTATGACGTCAATAAAGCGGCCAAAATGCCGTCCGcc > 1:668982/1‑140 (MQ=255) ctgcCAATGTGGTACATGGCGGAAGACCGTCTCGCCTGGTGGGATAAATTCTCCCAGCCGGCCGTGCGCCCCATCTATAGCCTCGGTATCGATACCTGGTGGTATGACGTCAATAAAGCGGCCAAAATGCCGTCCGCCAg > 2:459950/1‑140 (MQ=255) ctgcCAATGTGGTACATGGCGGAAGACCGTCTCGCCTGGTGGGATAAATTCTCCCAGCCGGCCGTGCGCCCCATCTATAGCCTCGGTATCGATACCTGGTGGTATGACGTCAATAAAGCGGCCAAAATGCCGTCCGCCAg < 2:4254/140‑1 (MQ=255) ctgcCAATGTGGTACATGGCGGAAGACCGTCTCGCCTGGTGGGATAAATTCTCCCAGCCGGCCGTGCGCCCCATCTATAGCCTCGGTATCGATACCTGGTGGTATGACGTCAATAAAGCGGCCAAAATGCCGTCCGCCAg > 1:107266/1‑140 (MQ=255) gcCAATGTGGTACATGGCGGAAGACCGTCTCGCCTGGTGGGATAAATTCTCCCAGCCGGCCGTGCGCCCCATCTATAGCCTCGGTATCGATACCTGGTGGTATGACGTCAATAAAGCGGCCAAAATGCCGTCCGCCAGCa > 1:490361/1‑140 (MQ=255) ccAATGTGGTACATGGCGGAAGACCGTCTCGCCTGGTGGGATAAATTCTCCCAGCCGGCCGTGCGCCCCATCTATAGCCTCGGTATCGATACCTGGTGGTATGACGTCAATAAAGCGGCCAAAATGCCGTCCGCCAGCaa > 2:77683/1‑140 (MQ=255) tgtgGCACATGGCGGAAGACCGTCTCGCCTGGTGGGATAAATTCTCCCAGCCGGCCGTGCGCCCCATCTATAGCCTCGGTATCGATACCCGGTGGTATGACGTCAATAAAGCGGCCAAAATGCCGTCCGCCAGCAAACAg < 2:668982/140‑1 (MQ=255) ggCGGAAGACCGTCTCGCCTGGTGGGATAAATTCTCCCAGCCGGCCGTGCGCCCCATCTATAGCCTCGGTATCGATACCTGGTGGTATGACGTCAATAAAGCGGCCAAAATGCCGTCCGCCAGCAAACAGGGAGAGTAGa > 2:77064/1‑140 (MQ=255) gCGGAAGACCGTCTCGCCTGGTGGGATAAATTCTCCCAGCCGGCCGTGCGCCCCATCTATAGCCTCGGTATCGATACCTGGTGGTATGACGTCAATAAAGCGGCCAAAATGCCGTCCGCCAGCAAACAGGGAGAGTAGAt < 1:77064/140‑1 (MQ=255) cGGAAGACCGTCTCGCCTGGTGGGATAAATTCTCCCAGCCGGCCGTGCGCCCCATCTATAGCCTCGGTATCGATACCTGGTGGTATGACGTCAATAAAGCGGCCAAAATGCCGTCCGCCAGCAAACAGGGAGAGTAGATg > 2:113624/1‑140 (MQ=255) cGGAAGACCGTCTCGCCTGGTGGGATAAATTCTCCCAGCCGGCCGTGCGCCCCATCTATAGCCTCGGTATCGATACCTGGTGGTATGACGTCAATAAAGCGGCCAAAATGCCGTCCGCCAGCAAACAGGGAGAGTAGATg > 1:198558/1‑140 (MQ=255) cGGAAGACCGTCTCGCCTGGTGGGATAAATTCTCCCAGCCGGCCGTGCGCCCCATCTATAGCCTCGGTATCGATACCTGGTGGTATGACGTCAATAAAGCGGCCAAAATGCCGTCCGCCAGCAAACAGGGAGAGTAGATg > 1:401507/1‑140 (MQ=255) cGGAAGACCGTCTCGCCTGGTGGGATAAATTCTCCCAGCCGGCCGTGCGCCCCATCTATAGCCTCGGTATCGATACCTGGGGGTATGACGTCAATAAAGCGGCCAAAATGCCGTCCGCCAGCACACAGGGAGAGTAGATg > 2:359184/1‑140 (MQ=255) tctcGCCTGGTGGGATAAATTCTCCCAGCCGGCCGTGCGCCCCATCTATAGCCTCGGTATCGATACCTGGTGGTATGACGTCAATAAAGCGGCCAAAGTGCCGTCCGCCAGCAAACAGGGAGAGTAGATGGGCGCTTAcc > 2:517262/1‑140 (MQ=255) tctcGCCTGGTGGGATAAATTCTCCCAGCCGGCCGTGCGCCCCATCTATAGCCTCGGTATCGATACCTGGTGGTATGACGTCAATAAAGCGGCCAAAATGCCGTCCGCCAGCAAACAGGGAGAGTAGATGGGCGCTTAcc > 2:395580/1‑140 (MQ=255) tctcGCCTGGTGGGATAAATTCTCCCAGCCGGCCGTGCGCCCCATCTATAGCCTCGGTATCGATACCTGGTGGTATGACGTCAATAAAGCGGCCAAAATGCCGTCCGCCAGCAAACAGGGAGAGTAGATGGGCGCTTAcc > 2:688586/1‑140 (MQ=255) tggGATAAATTCTCCCAGCCGGCCGTGCGCCCCATCTATAGCCTCGGTATCGATACCTGGTGGTATGACGTCAATAAAGCGGCCAAAATGCCGTCCGCCAGCAAACAGGGAGAGTAGATGGGCGCTTACCTGATTCGCCg < 1:463935/140‑1 (MQ=255) gggATAAATTCTCCCAGCCGGCCGTGCGCCCCATCTATAGCCTCGGTATCGATACCTGGTGGTATGACGTCAATAAAGCGGCCAAAATGCCGTCCGCCAGCAAACAGGGAGAGTAGATGGGCGCTTACCTGATTCGCCGt < 2:215308/140‑1 (MQ=255) ttCTCCCAGCCGGCCGTGCGCCCCATCTATAGCCTCGGTATCGATACCTGGTGGTATGACGTCAATAAAGCGGCCAAAATGCCGTCCGCCAGCAAACAGGGAGAGTAGATGGGCGCTTACCTGATTCGCCGTCTGTTGCt < 1:675759/140‑1 (MQ=255) gccggccgTGCGCCCCATCTATAGCCTCGGTATCGATACCTGGTGGTATGACGTCAATAAAGCGGCCAAAATGCCGTCCGCCAGCAAACAGGGAGAGTAGATGGGCGCTTACCTGATTCGCCGTCTGTTGCTGGTGATcc > 1:238456/1‑140 (MQ=255) ccggccgTGCGCCCCATCTATAGCCTCGGTATCGATACCTGGTGGTATGACGTCAATAAAGCGGCCAAAATGCCGTCCGCCAGCAAACAGGGAGAGTAGATGGGCGCTTACCTGATTCGCCGTCTGTTGCTGGTGATccc < 1:77683/140‑1 (MQ=255) ccggccgTGCGCCCCATCTATAGCCTCGGTATCGATACCTGGTGGTATGACGTCAATAAAGCGGCCAAAATGCCGTCCGCCAGCAAACAGGGAGAGTAGATGGGCGCTTACCTGATTCGCCGTCTGTTGCTGGTGATccc < 2:238456/140‑1 (MQ=255) gcgcCCCATCTATAGCCTCGGTATCGATACCTGGTGGTATGACGTCAATAAAGCGGCCAAAATGCCGTCCGCCAGCAAACAGGGAGAGTAGATGGGCGCTTACCTGATTCGCCGTCTGTTGCTGGTGATCCCAACATTAt < 2:620788/140‑1 (MQ=255) cgcCCCATCTATAGCCTCGGTATCGATACCTGGTGGTATGACGTCAATAAAGCGGCCAAAATGCCGTCCGCCAGCAAACAGGGAGAGTAGATGGGCGCTTACCTGATTCGCCGTCTGTTGCTGGTGATCCCAACATTATg < 2:190771/140‑1 (MQ=255) gcCCCATCTATAGCCTCGGTATCGATACCTGGTGGTATGACGTCAATAAAGCGGCCAAAATGCCGTCCGCCAGCAAACAGGGAGAGTAGATGGGCGCTTACCTGATTCGCCGTCTGTTGCTGGTGATCCCAACATTATgg < 1:60073/140‑1 (MQ=255) ccccATCTATAGCCTCGGTATCGATACCTGGTGGTATGACGTCAATAAAGCGGCCAAAATGCCGTCCGCCAGCAAACAGGGAGAGTAGATGGGCGCTTACCTGATTCGCCGTCTGTTGCTGGTGATCCCAACATTATggg < 2:198558/140‑1 (MQ=255) ccccATCTATAGCCTCGGTATCGATACCTGGTGGTACGACGTCAATAAAGCGGCCAAAATGCCGTCCGCCAGCAAACAGGGAGAGTAGATGGGCGCTTACCTGATTCGCCGTCTGTTGCTGGTGATCCCAACATTATggg < 1:170451/140‑1 (MQ=255) cccATCTATAGCCTCGGTATCGATACCTGGTGGTATGACGTCAATAAAGCGGCCAAAATGCCGTCCGCCAGCAAACAGGGAGAGTAGATGGGCGCTTACCTGATTCGCCGTCTGTTGCTGGTGATCCCAACATTATGGGc < 2:571062/140‑1 (MQ=255) tataGCCTCGGTATCGATTCCTGGTGGTAGGACGTCAATAGAGCGGCCAAAATGCCGGCCGCCAGCAAACAGGGAGAGTAGATGGGCGCTTACCTGATTCGCCGTCTGTTGCTGGTGATCCCATCATTATGGGCGATTAt < 2:158264/140‑1 (MQ=255) tCGGTATCGATACCTGGTGGTATGACGTCAATAAAGCGGCCAAAGTGCCGTCCGCCAGCAAACAGGGAGAGTAGATGGGCGCTTACCTGATTCGCCGTCTGTTGCTGGTGATCCCAACATTATGGGCGATTATCACCATc < 1:517262/140‑1 (MQ=255) tCGGTATCGATACCTGGTGGTATGACGTCAATAAAGCGGCCAAAATGCCGTCCGCCAGCAAACAGGGAGAGTAGATGGGCGCTTACCTGATTCGCCGTCTGTTGCTGGTGATCCCAACATTATGGGCGATTATCACCATc < 1:113624/140‑1 (MQ=255) tCGGTATCGATACCTGGTGGTATGACGTCAATAAAGCGGCCAAAATGCCGTCCGCCAGCAAACAGGGAGAGTAGATGGGCGCTTACCTGATTCGCCGTCTGTTGCTGGTGATCCCAACATTATGGGCGATTATCACCATc > 1:462256/1‑140 (MQ=255) cGGTATCGATACCTGGTGGTATGACGTCAATAAAGCGGCCAAAATGCCGTCCGCCAGCAAACAGGGAGAGTAGATGGGCGCTTACCTGATTCGCCGTCTGTTGCTGGTGATCCCAACATTATGGGCGATTATCACCATC‑a > 1:35920/1‑140 (MQ=255) cGGTATCGATACCTGGTGGTATGACGTCAATAAAGCGGCCAAAATGCCGTCCGCCAGCAAACAGGGAGAGTAGATGGGCGCTTACCTGATTCGCCGTCTGTTGCTGGTGATCCCAACATTATGGGCGATTATCACCATC‑a > 1:65543/1‑140 (MQ=255) ggTATCGATACCTGGTGGTATGACGTCAATAAAGCGGCCAAAATGCCGTCCGCCAGCAAACAGGGAGAGTAGATGGGCGCTTACCTGATTCGCCGTCTGTTGCTGGTGATCCCAACATTATGGGCGATTATCACCATC‑aa > 1:192281/1‑140 (MQ=255) aTACCTGGTGGTATGACGTCAATAAAGCGGCCAAAATCCCGTCCCCCAGAAAACAGGGGGGGAGGATGGCGCCTTACTTATTTGGCCGCCTGTTCCTGGGGATCCCACCATTTTGGCCGTTTAT‑ACCATCAAACTTTTTc > 2:629638/1‑140 (MQ=255) ccTGGTGGTATGACGTCAATAAAGCGGCCAAAATGCCGTCCGCCAGCAAACAGGGAGAGTAGATGGGCGCTTACCTGATTCGCCGTCTGTTGCTGGTGATCCCAACATTATGGGCGATTATCACCATC‑AACTTTTTCATc > 2:550354/1‑140 (MQ=255) gtggtATGACGTCAATAAAGCGGCCAAAATGCCGTCCGCCAGCAAACAGGGAGAGTAGATGGGCGCTTACCTGATTCGCCGTCTGTTGCTGGTGATCCCAACATTATGGGCGATTATCACCATC‑AACTTTTTCATCGTGc > 1:607043/1‑140 (MQ=255) gtATGACGTCAATAAAGCGGCCAAAATGCCGTCCGCCAGCAAACAGGGAGAGTAGATGGGCGCTTACCTGATTCGCCGTCTGTTGCTGGTGATCCCAACATTATGGGCGATTATCACCATC‑AACTTTTTCATCGTGCaaa > 2:293515/1‑140 (MQ=255) tCAATAAAGCGGCCAAAATGCCGTCCGCCAGCAAACAGGGAGAGTAGATGGGCGCTTACCTGATTCGCCGTCTGTTGCTGGTGATCCCAACATTATGGGCGATTATCACCATC‑AACTTTTTCATCGTGCAAATTGCGCCt > 1:499787/1‑140 (MQ=255) aaTAAAGCGGCCAAAATGCCGTCCGCCAGCAAACAGGGAGAGTAGATGGGCGCTTACCTGATTCGCCGTCTGTTGCTGGTGATCCCAACATTATGGGCGATTATCACCATC‑AACTTTTTCATCGTGCAAATTGCGCCTgg > 2:626655/1‑140 (MQ=255) tAAAGCGGCCAAAATGCCGTCCGCCAGCAAACAGGGAGAGTAGATGGGCGCTTACCTGATTCGCCGTCTGTTGCTGGTGATCCCAACATTATGGGCGATTATCACCATC‑AACTTTTTCATCGTGCAAATTGCGCCTGGcg < 2:462256/140‑1 (MQ=255) tAAAGCGGCCAAAATGCCGTCCGCCAGCAAACAGGGAGAGTAGATGGGCGCTTACCTGATTCGCCGTCTGTTGCTGGTGATCCCAACATTATGGGCGATTATCACCATC‑AACTTTTTCATCGTGCAAATTGCGCCTGGcg > 2:553000/1‑140 (MQ=255) aaGCGGCCAAAATGCCGTCCGCCAGCAAACAGGGAGAGTAGATGGGCGCTTACCTGATTCGCCGTCTGTTGCTGGTGATCCCAACATTATGGGCGATTATCACCATC‑AACTTTTTCATCGTGCAAATTGCGCCTGGcggt > 1:663750/1‑140 (MQ=255) ggCCAAAATGCCGTCCGCCAGCAAACAGGGAGAGTAGATGGGCGCTTACCTGATTCGCCGTCTGTTGCTGGTGATCCCAACATTATGGGCGATTATCACCATC‑AACTTTTTCATCGTGCAAATTGCGCCTGGcggtccgg < 1:407471/140‑1 (MQ=255) ggCCAAAATGCCGTCCGCCAGCAAACAGGGAGAGTAGATGGGCGCTTACCTGATTCGCCGTCTGTTGCTGGTGATCCCAACATTATGGGCGATTATCACCATC‑AACTTTTTCATCGTGCAAATTGCGCCTGGcggtccgg < 2:663750/140‑1 (MQ=255) | TGCTGCCAATGTGGTACATGGCGGAAGACCGTCTCGCCTGGTGGGATAAATTCTCCCAGCCGGCCGTGCGCCCCATCTATAGCCTCGGTATCGATACCTGGTGGTATGACGTCAATAAAGCGGCCAAACTGCCGTCCGCCAGCAAACAGGGAGAGTAGATGGGCGCTTACCTGATTCGCCGTCTGTTGCTGGTGATCCCAACATTATGGGCGATTATCACCATC‑AACTTTTTCATCGTGCAAATTGCGCCTGGCGGTCCGG > NC_000913/2274021‑2274281 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |