Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	2,483,187	C→A	T51T (ACG→ACT)	emrK ←	tripartite efflux pump membrane fusion protein EmrK

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	2,483,187	0	C	A	100.0%	106.9 / NA	32	T51T (ACG→ACT)	emrK	tripartite efflux pump membrane fusion protein EmrK
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base A (17/15);  total (17/15)

GCTTTATTGAGTGCGATAGTGGCATCAGTTTTATCCAGTGAAACTAAAATGTCACCTTGTCGAACGTAGTTCGTATCTTTATGATTAACGACAGTGACACTACCTGAGACTTGTGCAGAAATTGGATCTGCATTCCCCGTGACATAGGCGTCATCTGTACTAATCATGTCTTCTAATTCCATTGACCAATAGGCATAGGCACCTGAAAACGCAATAAATAAAACTACCGCCAATAAAGAAAAGTATTTTCTTCTGTTAGAATGTTTTTTAT  >  NC_000913/2483050‑2483320                                                                                                                                          |                                                                                                                                      gCTTTATTGAGTGCGATAGTGGCATCAGTTTTATCCAGTGAAACTAAAATGTCACCTTGTCGAACGTAGTTCGTATCTTTATGATTAACGACAGTGACACTACCTGAGACTTGTGCAGAAATTGGATCTGCATTCCCAGt                                                                                                                                     >  2:407506/1‑140 (MQ=255)     tATTGAGTGCGATAGTGGCATCAGTTTTATCCAGTGAAACTAAAATGTCACCTTGTCGAACGTAGTTCGTATCTTTATGATTAACGACAGTGACACTACCTGAGACTTGTGCAGAAATTGGATCTGCATTCCCAGTGACa                                                                                                                                 >  2:8574/1‑140 (MQ=255)          aGTGCGATAGTGGCATCAGTTTTATCCAGTGAAACTAAAATGTCACCTTGTCGAACGTAGTTCGTATCTTTATGATTAACGACAGTGACACTACCTGAGACTTGTGCAGAAATTGGATCTGCATTCCCAGTGACATAGGc                                                                                                                            <  2:386876/140‑1 (MQ=255)               gATAGTGGCATCAGTTTTATCCAGTGAAACTAAAATGTCACCTTGTCGAACGTAGTTCGTATCTTTATGATTAACGACAGTGACACTACCTGAGACTTGTGCAGAAATTGGATCTGCATTCCCAGTGACATAGGCGTCAt                                                                                                                       <  2:456839/140‑1 (MQ=255)                    tGGCATCAGTTTTATCCAGTGAAACTAAAATGTCACCTTGTCGAACGTAGTTCGTATCTTTATGATTAACGACAGTGACACTACCTGAGACTTGTGCAGAAATTGGATCTGCATTCCCAGTGACATAGGCGTCATCTGTa                                                                                                                  >  1:375974/1‑140 (MQ=255)                        aTCAGTTTTATCCAGTGAAACTAAAATGTCACCTTGTCGAACGTAGTTCGTATCTTTATGATTAACGACAGTGACACTACCTGAGACTTGTGCAGAAATTGGATCTGCATTCCCAGTGACATAGGCGTCATCTGTACTaa                                                                                                              <  2:188571/140‑1 (MQ=255)                             ttttATCCAGTGAAACTAAAATGTCACCTTGTCGAACGTAGTTCGTATCTTTATGATTAACGACAGTGACACTACCTGAGACTTGTGCAGAAATTGGATCTGCATTCCCAGTGACATAGGCGTCATCTGTACTAATCATg                                                                                                         <  1:186620/140‑1 (MQ=255)                                  tCCAGTGAAACTAAAATGTCACCTTGTCGAACGTAGTTCGTATCTTTATGATTAACGACAGTGACACTACCTGAGACTTGTGCAGAAATTGGATCTGCATTCCCAGTGACATAGGCGTCATCTGTACTAATCATGtcttc                                                                                                    >  1:422843/1‑140 (MQ=255)                                    cAGTGAAACTAAAATGTCACCTTGTCGAACGTAGTTCGTATCTTTATGATTAACGACAGTGACACTACCTGAGACTTGTGCAGAAATTGGATCTGCATTCCCAGTGACATAGGCGTCATCTGTACTAATCATGtcttctt                                                                                                  <  2:29886/140‑2 (MQ=255)                                     aGTGAAACTAAAATGTCACCTTGTCGAACGTAGTTCGTATCTTTATGATTAACGACAGTGACACTACCTGAGACTTGTGCAGAAATTGGATCTGCATTCCCAGTGACATAGGCGTCATCTGTACTAATCATGTCTTCTaa                                                                                                 >  1:96723/1‑140 (MQ=255)                                            cTAAAATGTCACCTTGTCGAACGTAGTTCGTATCTTTATGATTAACGACAGTGACACTACCTGAGACTTGTGCAGAAATTGGATCTGCATTCCCAGTGACATAGGCGTCATCTGTACTAATCATGTCTTCTAATTCCAtt                                                                                          <  1:116268/140‑1 (MQ=255)                                            cTAAAATGTCACCTTGTCGAACGTAGTTCGTATCTTTATGATTAACGACAGTGACACTACCTGAGACTTGTGCAGAAATTGGATCTGCATTCCCAGTGACATAGGCGTCATCTGTACTAATCATGTCTTCTAATTCCAtt                                                                                          <  1:407506/140‑1 (MQ=255)                                                 aTGTCACCTTGTCGAACGTAGTTCGTATCTTTATGATTAACGACAGTGACACTACCTGAGACTTGTGCAGAAATTGGATCTGCATTCCCAGTGACATAGGCGTCATCTGTACTAATCATGTCTTCTAATTCCATTGGCCa                                                                                     <  1:71940/140‑1 (MQ=255)                                                 aTGTCACCTTGTCGAACGTAGTTCGTATCTTTATGATTAACGACAGTGACACTACCTGAGACTTGTGCAGAAATTGGATCTGCATTCCCAGTGACATAGGCGTCATCTGTACTAATCATGTCTTCTAATTCCATTGACCa                                                                                     >  1:158771/1‑140 (MQ=255)                                                  tGTCTCGTTGTCGAACGTAGTTCGTATCTTTATGATTAACGACAGTGACACTACCTGAGACTTGTGCAGAAATTGGATCTGCATTTCCAGTGACATAGGCGTCATCTGTTCTAATCATGTCTTCTAATTCCATTGACCaa                                                                                    <  1:357464/140‑1 (MQ=255)                                                    tCACCTTGTCGAACGTAGTTCGTATCTTTATGATTAACGACAGTGACACTACCTGAGACTTGTGCAGAAATTGGATCTGCATTCCCAGTGACATAGGCGTCATCTGTACTAATCATGTCTTCTAATTCCATTGACCAata                                                                                  <  2:228178/140‑1 (MQ=255)                                                                         gTATCTTTATGATTAACGACAGTGACACTACCTGAGACTTGTGCAGAAATTGGATCTGCATTCCCAGTGACATAGGCGTCATCTGTACTAATCATGTCTTCTAATTCCATTGACCAATAGGCATAGGCACCTGAAAACGc                                                             <  2:577428/140‑1 (MQ=255)                                                                          tATCTTTATGATTAACGACAGTGACACTACCTGAGACTTGTGCAGAAATTGGATCTGCATTCCCAGTGACATAGGCGTCATCTGTACTAATCATGTCTTCTAATTCCATTGACCAATAGGCATAGGCACCTGAAAACGCa                                                            <  2:181021/140‑1 (MQ=255)                                                                          tATCTTTATGATTAACGACAGTGACACTACCTGAGACTTGTGCAGAAATTGGATCTGCATTCCCAGTGACATAGGCGTCATCTGTACTAATCATGTCTTCTAATTCCATTGACCAATAGGCATAGGCACCTGAAAACGCa                                                            <  2:251286/140‑1 (MQ=255)                                                                          tATCTTTATGATTAACGACAGTGACACTACCTGAGACTTGTGCAGAAATTGGATCTGCATTCCCAGTGACATAGGCGTCATCTGTACTAATCATGTCTTCTAATTCCATTGACCAATAGGCATAGGCACCTGAAAACGCa                                                            <  2:34382/140‑1 (MQ=255)                                                                                tATGATTAACGACAGTGACACTACCTGAGACTTGTGCAGAAATTGGATCTGCATTCCCAGTGACATAGGCGTCATCTGTACTAATCATGTCTTCTAATTCCATTGACCAATAGGCATAGGCACCTGAAAACGCaataaat                                                      >  2:25843/1‑140 (MQ=255)                                                                                        aCGACAGTGACACTACCTGAGACTTGTGCAGAAATTGGATCTGCATTCCCAGTGACATAGGCGTCATCTGTACTAATCATGTCTTCTAATTCCATTGACCAATAGGCATAGGCACCTGAAAACGCAATAAATAAAACTAc                                              >  1:172510/1‑140 (MQ=255)                                                                                        aCGACAGTGACACTACCTGAGACTTGTGCAGAAATTGGATCTGCATTCCCAGTGACATAGGCGTCATCTGTACTAATCATGTCTTCTAATTCCATTGACCAATAGGCATAGGCACCTGAAAACGCAATAAATAAAACTAc                                              >  1:614123/1‑140 (MQ=255)                                                                                               tGACACTACCTGAGACTTGTGCAGAAATTGGATCTGCATTCCCAGTGACATAGGCGTCATCTGTACTAATCATGTCTTCTAATTCCATTGACCAATAGGCATAGGCACCTGAAAACGCAATAAATAAAACTACCGCCAAt                                       >  2:357601/1‑140 (MQ=255)                                                                                               tGACACTACCTGAGACTTGTGCAGAAATTGGATCTGCATTCCCAGTGACATAGGCATCATCTGTACTAATCATGTCTTCTAATTCCATTGACCAATAGGCATAGGCACCTGAAAACGCAATAAATAAAACTACCGCCAAt                                       >  1:243063/1‑140 (MQ=255)                                                                                                   acTACCTGAGACTTGTGCAAAAATTGGATCTGCATTCCCAGTGACATAGGCGTCATCTGTACTAATCATGTCTTCTAATTCCATTGACCAATAGGCATAGGCACCTGAAAACGCAATAAATAAAACTACCGCCAATAAAg                                   <  2:552127/140‑1 (MQ=255)                                                                                                       ccTGAGACTTGCGCAGAAATTGGATCTGCATTCCCAGTGACATAGGCGTCATCTGTACTAATCATGTCTTCTAATTCCATTGACCAATAGGCATAGGCACCTGAAAACGCAATAACTAAAACTACCGCCAATAACGaaaa                               >  2:522422/1‑140 (MQ=255)                                                                                                           agaCTTGTGCAGAAATTGGATCTGCATTCCCAGTGACATAGGCGTCATCTGTACTAATCATGTCTTCTAATTCCATTGACCAATAGGCATAGGCACCTGAAAACGCAATAAATAAAACTACCGCCAATAAAGAAAAGTAt                           >  1:92921/1‑140 (MQ=255)                                                                                                             aCTTGTGCAGAAATTGGATCTGCATTCCCAGTGACATAGGCGTCATCTGTACTAATCATGTCTTCTAATTCCATTGACCAATAGGCATAGGCACCTGAAAACGCAATAAATAAAACTACCGCCAATAAAGAAAAGTAttt                         >  2:423650/1‑140 (MQ=255)                                                                                                                 gtgCAGAAATTGGATCTGCATTCCCAGTGACATAGGCGTCATCTGTACTAATCATGTCTTCTAATTCCATTGACCAATAGGCATAGGCACCTGAAAACGCAATAAATAAAACTACCGCCAATAAAGAAAAGTATTttctt                     >  1:539131/1‑140 (MQ=255)                                                                                                                 gtgCAGAAATTGGATCTGCATTCCCAGTGACATAGGCGTCATCTGTACTAATCATGTCTTCTAATTCCATTGACCAATAGGCATAGGCACCTGAAAACGCAATAAATAAAACTACCGCCAATAAAGAAAAGTATTttctt                     >  1:299685/1‑140 (MQ=255)                                                                                                                 gtgCAGAAATTGGATCTGCATTCCCAGTGACATAGGCGTCATCTGTACTAATCATGTCTTCTAATTCCATTGACCAATAGGCATAGGCACCTGAAAACGCAATAAATAAAACTACCGCCAATAAAGAAAAGTATTttctt                     >  2:504388/1‑140 (MQ=255)                                                                                                                  tgCAAAAATTGGATCTGCATCCCCAGTGACATAGGCGTCATCTGTACTAATCATGTCTTCTAATTCCATTGACAAATAGGAATAGGCCCCTAAAAACGAAATAAATAAAACTACCGCCAATAAAGAAAAGTATTttcttc                    <  2:55441/140‑1 (MQ=255)                                                                                                                                    aTTCCCAGTGACATAGGCGTCATCTGTACTAATCATGTCTTCTAATTCCATTGACCAATAGGCATAGGCACCTAAAAACGCAAAAAATAAAACTACCGCCAATAAAGAAAAGTATTTTCTTCTGTTAGAATGTTTTTTAt  <  2:65048/140‑1 (MQ=255)                                                                                                                                          |                                                                                                                                      GCTTTATTGAGTGCGATAGTGGCATCAGTTTTATCCAGTGAAACTAAAATGTCACCTTGTCGAACGTAGTTCGTATCTTTATGATTAACGACAGTGACACTACCTGAGACTTGTGCAGAAATTGGATCTGCATTCCCCGTGACATAGGCGTCATCTGTACTAATCATGTCTTCTAATTCCATTGACCAATAGGCATAGGCACCTGAAAACGCAATAAATAAAACTACCGCCAATAAAGAAAAGTATTTTCTTCTGTTAGAATGTTTTTTAT  >  NC_000913/2483050‑2483320

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 20 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑