Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	2,522,486	G→T	intergenic (‑9/+243)	xapR ← / ← xapB	DNA‑binding transcriptional activator XapR/xanthosine:H(+) symporter XapB

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	2,522,486	0	G	T	100.0%	178.1 / NA	52	intergenic (‑9/+243)	xapR/xapB	DNA‑binding transcriptional activator XapR/xanthosine:H(+) symporter XapB
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base T (24/28);  total (24/28)

TTTAATATGAATGCTGAGCGGAGGCTGAGACATATTTAAACGCGCTGCTGCGCGGCCAAAATGCAACTCTTCCGCTACGGCAAGAAAATAACGGAGCAACTTAAGATCTGTTCTGTATACGCGTTCCATAATTAAGTGCTCCATATAATAAATACATCGTATTAAATTATCATGGCATAAAGTATTTCACCACCAGATATCCGACATACATAAACACAATGGATAATATACCCGGTAATACATTTGGTACAGGAATAGGCAAGCGCACGATT  >  NC_000913/2522349‑2522620                                                                                                                                          |                                                                                                                                       tttAATATGAATGCTGAGCGGAGGCTGAGACATATTTAAACGCGCTGCTGCGCGGCCAAAATGCAACTCTTCCGCTACGGCAAGAAAATAACGGAGCAACTTAAGATCTGTTCTGTATACGCGTTCCATAATTAAGTTCt                                                                                                                                      <  1:635931/140‑1 (MQ=255)  ttAATATGAATGCTGAGCGGAGGCTGAGACATATTTAAACGCGCTGCTGCGCGGCCAAAATGCAACTCTTCCGCTACGGCAAGAAAATAACGGAGCAACTTAAGATCTGTTCTGTATACGCGTTCCATAATTAAGTtctc                                                                                                                                     <  1:149995/140‑1 (MQ=255)     atatGAATGCTGAGCGGAGGCTGGGACATATTTAAACGCGCTGCTGCGCGGCCAAAATGCAACTCTTCCGCTACGGCAAGAAAATAACGGAGCAACTTAAGATCTGTTCTGTATACGCGTTCCATAATTAAGTTCTCCat                                                                                                                                  <  2:348147/140‑1 (MQ=255)     atatGAATGCTGAGCGGAGGCTGAGACATATTTAAACGCGCTGCTGCGCGGCCAAAATGCAACTCTTCCGCTACGGCAAGAAAATAACGGAGCAACTTAAGATCTGTTCTGTATACGCGTTCCATAATTAAGTTCTCCat                                                                                                                                  <  1:111138/140‑1 (MQ=255)        tGAATGCTGAGCGGAGGCTGAGACATATTTAAACGCGCTGCTGCGCGGCCAAAATGCAACTCTTCCGCTACGGCAAGAAAATAACGGAGCAACTTAAGATCTGTTCTGTATACGCGTTCCATAATTAAGTTCTCCAtata                                                                                                                               <  1:20688/140‑1 (MQ=255)         gAATGCTGAGCGGAGGCTGAGACATATTTAAACGCGCTGCTGCGCGGCCGAAATGCAACTCTTCCGCTACGGCAAGAAAATAACGGAGCAACTTAAGATCTGTTCTGTATACGCGTTCCATAATTAAGTTCTCCATataa                                                                                                                              >  1:585568/1‑140 (MQ=255)           aTGCTGGGCGGAGGCTGAGACATATTTAAACGCGCTGCTGCGCGGCCAAAATGCAACTCTTCCGCTACGGCAAGAAAATAACGGAGCAACTTAAGATCTGTTCTGTATACGCGTTCCATAATTAAGTTCTCCATataata                                                                                                                            >  2:680982/1‑140 (MQ=255)           aTGCTGAGCGGAGGCTGAGACATATTTAAACGCGCTGCTGCGCGGCCAAAATGCAACTCTTCCGCTACGGCAAGAAAATAACGGAGCAACTTAAGATCTGTTCTGTATACGCGTTCCATAATTAAGTTCTCCATataata                                                                                                                            >  2:621109/1‑140 (MQ=255)           aTGCTGAGCGGAGGCTGAGACATATTTAAACGCGCTGCTGCGCGGCCAAAATGCAACTCTTCCGCTACGGCAAGAAAATAACGGAGCAACTTAAGATCTGTTCTGTATACGCGTTCCATAATTAAGTTCTCCATataata                                                                                                                            >  2:205728/1‑140 (MQ=255)           aTGCTGAGCGGAGGCTGAGACATATTTAAACGCGCTGCTGCGCGGCCAAAATGCAACTCTTCCGCTACGGCAAGAAAATAACGGAGCAACTTAAGATCTGTTCTGTATACGCGTTCCATAATTAAGTTCTCCATataata                                                                                                                            >  1:421617/1‑140 (MQ=255)                 aGCGGAGGCTGAGACATATTTAAACGCGCTGCTGCGCGGCCAAAATGCAACTCTTCCGCTACGGCAAGAAAATAACGGAGCAACTTAAGATCTGTTCTGTATACGCGTTCCATAATTAAGTTCTCCATATAATAAATACa                                                                                                                      <  1:244317/140‑1 (MQ=255)                   cGGAGGCTGAGACATATTTAAACGCGCTGCTGCGCGGCCAAAATGCAACTCTTCCGCTACGGCAAGAAAATAACGGAGCAACTTAAGATCTGTTCTGTATACGCGTTCCATAATTAAGTTCTCCATATAATAAATACATc                                                                                                                    >  2:496521/1‑140 (MQ=255)                      aGGCTGAGACATATTTAAACGCGCTGCTGCGCGGCCAAAATGCAACTCTTCCGCTACGGCAAGAAAATAACGGAGCAACTTAAGATCTGTTCTGTATACGCGTTCCATAATTAAGTTCTCCATATAATAAATACATCGTa                                                                                                                 <  1:169475/140‑1 (MQ=255)                       ggCTGAGACATATTTAAACGCGCTGCTGCGCGGCCAAAATGCAACTCTTCCGCTACGGCAAGAAAATAACGGAGCAACTTAAGATCTGTTCTGTATACGCGTTCCATAATTAAGTTCTCCATATAATAAATACATCGTAt                                                                                                                >  2:349038/1‑140 (MQ=255)                                 tatTTAAACGCGCTGCTGCGCGGCCAAAATGCAACTCTTCCGCTACGGCAAGAAAATAACGGAGCAACTTAAGATCTGTTCTGTATACGCGTTCCATAATTAAGTTCTCCATATAATAAATACATCGTATTAAATTATCa                                                                                                      <  2:64721/140‑1 (MQ=255)                                  atTTAAACGCGCTGCTGCGCGGCCAAAATGCAACTCTTCCGCTACGGCAAGAAAATAACGCACCAACTTAAGATCTGTTCTGTATACGCGTTCCATAATTAAGTTCTCCATATAATAAATACATCGTATTAAATTATCAt                                                                                                     >  1:194852/1‑140 (MQ=255)                                      aaaCGCGCTGCTGCGCGGCCAAAATGCAACTCTTCCGCTACGGCAAGAAAATAACGGAGCAACTTAAGATCTGTTCTGTATACGCGTTCCATAATTAAGTTCTCCATATAATAAATACATCGTATTAAATTATCATGGCa                                                                                                 <  1:189116/140‑1 (MQ=255)                                      aaaCGCGCTGCTGCGCGGCCAAAATGCAACTCTTCCGCTACGGCAAGAAAATAACGGAGCAACTTAAGATCTGTTCTGTATACGCGTTCCATAATTAAGTTCTCCATATAATAAATACATCGTATTAAATTATCATGGCa                                                                                                 <  1:131783/140‑1 (MQ=255)                                        aCGCGCTGCTGCGCGGCCAAAATGCAACTCTTCCGCTACGGCAAGAAAATAACGGAGCAACTTAAGATCTGTTCTGTATACGCGTTCCATAATTAAGTTCTCCATATAATAAATACATCGTATTAAATTATCATGGCATa                                                                                               <  2:393361/140‑1 (MQ=255)                                         cgcgCTGCTGCGCGGCCAAAATGCAACTCTTCCGCTACGGCAAGAAAATAACGGAGCAACTTAAGATCTGTTCTGTATACGCGTTCCATAATTAAGTTCTCCATATAATAAATACATCGTATTAAATTATCATGGCATaa                                                                                              <  2:470106/140‑1 (MQ=255)                                           cgcTGCTGCGCGGCCAAAATGCAACTCTTCCGCTACGGCAAGAAAATAACGGAGCAACTTAAGATCTGTTCTGTATACGCGTTCCATAATTAAGTTCTCCATATAATAAATACATCGTATTAAATTATCATGGCATAAAg                                                                                            <  2:568555/140‑1 (MQ=255)                                             ctgctgCGCGGCCAAAATGCAACTCTTCCGCTACGGCAAGAAAATAACGGAGCAACTTAAGATCTGTTCTGTATACGCGTTCCATAATTAAGTTCTCCATATAATAAATACATCGTATTAAATTATCATGGCATAAAGTa                                                                                          >  2:645604/1‑140 (MQ=255)                                             ctgctgCGCGGCCAAAATGCAACTCTTCCGCTACGGCAAGAAAATAACGGAGCAACTTAAGATCTGTTCTGTATACGCGTTCCATAATTAAGTTCTCCATATAATAAATACATCGTATTAAATTATCATGGCATAAAGTa                                                                                          >  2:52428/1‑140 (MQ=255)                                                                  aCTCTTCCGCTACGGCAAGAAAATAACGGAGCAACTTAAGATCTGTTCTGTATACGCGTTCCATAATTAAGTTCTCCATATAATAAATACATCGTATTAAATTATCATGGCATAAAGTATTTCACCACCAGATATCCGac                                                                     >  2:74399/1‑140 (MQ=255)                                                                     ctTCCGCTACGGCAAGAAAATAACGGAGCAACTTAAGATCTGTTCTGTATACGCGTTCCATAATTAAGTTCTCCATATAATAAATACATCGTATTAAATTATCATGGCATAAAGTATTTCACCACCAGATATCCGacata                                                                  <  2:32604/140‑1 (MQ=255)                                                                           cTACGGCAAGAAAATAACGGAGCAACTTAAGATCTGTTCTGTATACGCGTTCCATAATTAAGTTCTCCATATAATAAATACATCGTATTAAATTATCATGGCATAAAGTATTTCACCACCAGATATCCGACATACATaaa                                                            <  2:289676/140‑1 (MQ=255)                                                                           cTACGGCAAGAAAATAACGGAGCAACTTAAGATCTGTTCTGTATACGCGTTCCATAATTAAGTTCTCCATATAATAAATACATCGTATTAAATTATCATGGCATAAAGTATTTCACCACCAGATATCCGACATACATaaa                                                            >  1:517076/1‑140 (MQ=255)                                                                             aCGGCAAGAAAATAACGGAGCAACTTAAGATCTGTTCTGTATACGCGTTCCATAATTAAGTTCTCCATATAATAAATACATCGTATTAAATTATCATGGCATAAAGTATTTCACCACCAGATATCCGACATACATAAaca                                                          <  2:432526/140‑1 (MQ=255)                                                                                 cAAGAAAATAACGGAGCAACTTAAGATCTGTTCTGTATACGCGTTCCATAATTAAGTTCTCCATATAATAAATACATCGTATTAAATTATCATGGCATAAAGTATTTCACCACCAGATATCCGACATACATAAACACAAt                                                      <  1:680982/140‑1 (MQ=255)                                                                                 cAAGAAAATAACGGAGCAACTTAAGATCTGTTCTGTATACGCGTTCCATAATTAAGTTCTCCATATAATAAATACATCGTATTAAATTATCATGGCATAAAGTATTTCACCACCAGATATCCGACATACATAAACACAAt                                                      <  1:645604/140‑1 (MQ=255)                                                                                               aGCAACTTAAGATCTGTTCTGTATACGCGTTCCATAATTAAGTTCTCCATATAATAAATACATCGTATTAAATTATCATGGCATAAAGTATTTCACCACCAGATATCCGACATACATAAaca                                                          >  2:235589/1‑122 (MQ=255)                                                                                               aGCAACTTAAGATCTGTTCTGTATACGCGTTCCATAATTAAGTTCTCCATATAATAAATACATCGTATTAAATTATCATGGCATAAAGTATTTCACCACCAGATATCCGACATACATAAaca                                                          <  1:235589/122‑1 (MQ=255)                                                                                               aGCAACTTAAGATCTGTTCTGTATACGCGTTCCATAATTAAGTTCTCCATATAATAAATACATCGTATTAAATTATCATGGCATAAAGTATTTCACCACCAGATATCCGACATACATAAACACAATGGATAATATACCCg                                        >  2:658345/1‑140 (MQ=255)                                                                                               aGCAACTTAAGATCTGTTCTGTATACGCGTTCCATAATTAAGTTCTCCATATAATAAATACATCGTATTAAATTATCATGGCATAAAGTATTTCACCACCAGATATCCGACATACATAAACACAATGGATAATATACCCg                                        >  1:391575/1‑140 (MQ=255)                                                                                                             tgttctgtATACGCGTTCCATAATTAAGTTCTCCATATAATAAATACATCGTATTAAATTATCATGGCATAAAGTATTTCACCACCAGATATCCGACATACATAAACACAATGGATAATATACCCGGTAATACATTTGGt                          <  1:621109/140‑1 (MQ=255)                                                                                                               ttctgtATACGCGTTCCATAATTAAGTTCTCCATATAATACATACATCGTATTAAATTATCATGGCATAAAGTATTTCACCACCAGATATCCGACATACATAAACACAATGGATAATATACCCGGTAATACATTTGGTAc                        >  2:340451/1‑140 (MQ=255)                                                                                                               ttctgtATACGCGTTCCATAATTAAGTTCTCCATATAATAAATACATCGTATTAAATTATCATGGCATAAAGTATTTCACCACCAGATATCCGACATACATAAACACAATGGATAATATCCCCGGTAATACATTTGGTAc                        <  2:700402/140‑1 (MQ=255)                                                                                                               ttctgtATACGCGTTCCATAATTAAGTTCTCCATATAATAAATACATCGTATTAAATTATCATGGCATAAAGTATTTCACCACCAGATATCCGACATACATAAACACAATGGATAATATACCCGGTAATACATTTGGTAc                        >  2:537834/1‑140 (MQ=255)                                                                                                               ttctgtATACGCGTTCCATAATTAAGTTCTCCATATAATAAATACATCGTATTAAATTATCATGGCATAAAGTATTTCACCACCAGATATCCGACATACATAAACACAATGGATAATATACCCGGTAATACATTTGGTAc                        >  2:532618/1‑140 (MQ=255)                                                                                                                 ctgtATACGCGTTCCATAATTAAGTTCTCCATATAATAAATACATCGTATTAAATTATCATGGCATAAAGTATTTCACCACCAGATATCCGACATACATAAACACAATGGATAATATACCCGGTAATACATTTGGTACAg                      <  1:496521/140‑1 (MQ=255)                                                                                                                      taCGCGTTCCATAATTAAGTTCTCCATATAATAAATACATCGTATTAAATTATCATGGCATAAAGTATTTCACCACCAGATATCCGACATACATAAACACAATGGATAATATACCCGGTAATACATTTGGTACAGGAATa                 >  1:372999/1‑140 (MQ=255)                                                                                                                         gcgTTCCATAATTAAGTTCTCCATATAATAAATACATCGTATTAAATTATCATGGCATAAAGTATTTCACCACCAGATATCCGACATACATAAACACAATGGATAATATACCCGGTAATACATTTGGTACAGGAATAGGc              <  1:340451/140‑1 (MQ=255)                                                                                                                         gcgTTCCATAATTAAGTTCTCCATATAATAAATACATCGTATTAAATTATCATGGCATAAAGTATTTCACCACCAGATATCCGACATACATAAACACAATGGATAATATACCCGGTAATACATTTGGTACAGGAATAGGc              <  1:318820/140‑1 (MQ=255)                                                                                                                         gcgTTCCATAATTAAGTTCTCCATATAATAAATACATCGTATTAAATTATCATGGCATAAAGTATTTCACCACCAGATATCCGACATACATAAACACAATGGATAATATACCCGGTAATACATTTGGTACAGGAATAGGc              <  1:85473/140‑1 (MQ=255)                                                                                                                            ttCCATAATTAAGTTCTCCATATAATAAATACATCGTATTAAATTATCATGGCATAAAGTATTTCACCACCAGATATCCGACATACATAAACACAATGGATAATATACCCGGTAATACATTTGGTACAGGAATAGGCAAg           >  1:142842/1‑140 (MQ=255)                                                                                                                              ccATAATTAAGTTCTCCATATAATAAATACATCGTATTAAATTATCATGGCATAAAGTATTTCACCACCAGATATCCGACATACATAAACACAATGGATAATATACCCGGTAATACATTTGGTACAGGAATAGGCAAgcg         >  2:621173/1‑140 (MQ=255)                                                                                                                              ccATAATTAAGTTCTCCATATAATAAATACATCGTATTAAATTATCATGGCATAAAGTATTTCACCACCAGATATCCGACATACATAAACACAATGGATAATATACCCGGTAATACATTTGGTACAGGAATAGGCAAgcg         >  1:228749/1‑140 (MQ=255)                                                                                                                              ccATAATTAAGTTCTCCATATAATAAATACATCGTATTAAATTATCATGGCATAAAGTATTTCACCACCAGATATCCGACATACATAAACACAATGGATAATATACCCGGTAATACATTTGGTACAGGAATAGGCAAgcg         >  2:228909/1‑140 (MQ=255)                                                                                                                              ccATAATTAAGTTCTCCATATAATAAATACATCGTATTAAATTATCATGGCATAAAGTATTTCACCACCAGATATCCGACATACATAAACACAATGGATAATATACCCGGTAATACATTTGGTACAGGAATAGGCAAgcg         >  1:144460/1‑140 (MQ=255)                                                                                                                                aTAATTAAGTTCTCCATATAATAAATACATCGTATTAAATTATCATGGCATAAAGTATTTCACCACCAGATATCCGACATACATAAACACAATGGATAATATACCCGGTAATACATTTGGTACAGGAATAGGCAAGCGCa       <  2:517076/140‑1 (MQ=255)                                                                                                                                     tAAGTTCTCCATATAATAAATACATCGTATTAAATTATCATGGCATAAAGTATTTCACCACCAGATATCCGACATACATAAACACAATGGATAATATACCCGGTAATACATTTGGTACAGGAATAGGCAAGCGCACGAtt  <  2:391575/140‑1 (MQ=255)                                                                                                                                     tAAGTTCTCCATATAATAAATACATCGTATTAAATTATCATGGCATAAAGTATTTCACCACCAGATATCCGACATACATAAACACAATGGATAATATACCCGGTAATACATTTGGTACAGGAATAGGCAAGCGCACGAtt  <  1:55361/140‑1 (MQ=255)                                                                                                                                       aGTTCTCCATATAATAAATACATCGTATTAAATTATCATGGCATAAAGTATTTCACCACCAGATATCCGACATACATAAACACAATGGATAATATACCCGGTAATACATTTGGTACAGGAATAGGCAAGCGCa       <  1:537380/133‑1 (MQ=255)                                                                                                                                          |                                                                                                                                       TTTAATATGAATGCTGAGCGGAGGCTGAGACATATTTAAACGCGCTGCTGCGCGGCCAAAATGCAACTCTTCCGCTACGGCAAGAAAATAACGGAGCAACTTAAGATCTGTTCTGTATACGCGTTCCATAATTAAGTGCTCCATATAATAAATACATCGTATTAAATTATCATGGCATAAAGTATTTCACCACCAGATATCCGACATACATAAACACAATGGATAATATACCCGGTAATACATTTGGTACAGGAATAGGCAAGCGCACGATT  >  NC_000913/2522349‑2522620

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑