Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	3,340,494	C→A	L74I (CTC→ATC)	yrbG →	putative transport protein YrbG

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	3,340,494	0	C	A	100.0%	190.9 / NA	54	L74I (CTC→ATC)	yrbG	putative transport protein YrbG
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base A (21/33);  total (21/33)

TGCCGAACCTTTGGCATCCCGCCGCTGATCATCGGCATGACGGTGGTCAGTATTGGTACATCGTTACCAGAAGTCATCGTCTCGCTTGCCGCGTCTCTGCACGAACAACGCGATTTAGCCGTTGGTACAGCCCTCGGCTCAAACATTATCAATATATTGCTGATCCTCGGTCTGGCTGCGCTGGTTCGTCCTTTTACCGTCCATTCTGATGTTCTACGCCGTGAATTACCCTTAATGTTGTTGGTCAGCGTGGTGGCCGGTTC  >  NC_000913/3340362‑3340624                                                                                                                                     |                                                                                                                                   tGCCGAACCTTTGGCATCCCGCCGCTGATCATCGGCATGACGGTGGTCAGTATTGGTACATCGTTACCAGAAGTCATCGTCTCGCTTGCCGCGTCTCTGCACGAACAACGCGATTTAGCCGTTGGTACAGCCATCGGCTc                                                                                                                             >  1:165950/1‑140 (MQ=255) tGCCGAACCTTTGGCATCCCGCCGCTGATCATCGGCATGACGGTGGTCAGTATTGGTACATCGTTACCAGAAGTCATCGTCTCGCTTGCCGCGTCTCTGCACGAACAACGCGATTTAGCCGTTGGTACAGCCATCGGCTc                                                                                                                             >  1:698887/1‑140 (MQ=255)   ccGAACCTTTGGCATCCCGCCGCTGATCATCGGCATGACGGTGGTCAGTATTGGTACATCGTTACCAGAAGTCATCGTCTCGCTTGCCGCGTCTCTGCACGAACAACGCGATTTAGCCGTTGGTACAGCCATCGGCTCaa                                                                                                                           <  1:408092/140‑1 (MQ=255)     gAACCTTTGGCATCCCGCCGCTGATCATCGGCATGACGGTGGTCAGTATTGGTACATCGTTACCAGAAGTCATCGTCTCGCTTGCCGCGTCTCTGCACGAACAACGCGATTTAGCCGTTGGTACAGCCATCGGCTCAAAc                                                                                                                         <  1:235310/140‑1 (MQ=255)     gAACCTTTGGCATCCCGCCGCTGATCATCGGCATGACGGTGGTCAGTATTGGTACATCGTTACCAGAAGTCATCGTCTCGCTTGCCGCGTCTCTGCACGAACAACGCGATTTAGCCGTTGGTACAGCCATCGGCTCAAAc                                                                                                                         <  2:470382/140‑1 (MQ=255)     gAACCTTTGGCATCCCGCCGCTGATCATCGGCATGACGGTGGTCAGTATTGGTACATCGTTACCAGAAGTCATCGTCTCGCTTGCCGCGTCTCTGCACGAACAACGCGATTTAGCCGTTGGTACAGCCATCGGCTCAAAc                                                                                                                         <  1:673381/140‑1 (MQ=255)        ccTTTGGCATCCCGCCGCTGATCATCGGCATGACGGTGGTCAGTATTGGTACATCGTTACCAGAAGTCATCGTCTCGCTTGCCGCGTCTCTGCACGAACAACGCGATTTAGCCGTTGGTACAGCCATCGGCTCAAACAtt                                                                                                                      <  1:63776/140‑1 (MQ=255)          tttGGCATCCCGCCGCTGATCATCGGCATGACGGTGGTCAGTATTGGTACATCGTTACCAGAAGTCATCGTCTCGCTTGCCGCGTCTCTGCACGAACAACGCGATTTAGCCGTTGGTACAGCCATCGGCTCAAACATTAt                                                                                                                    <  2:472360/140‑1 (MQ=255)            tggccgcccGCCGCTGATCATCGGCATGACGGTGGTCAGTATTGGTACATCGTTACCAGAAGTCATCGTCTCGCTTGCCGCGTCTCTGCACGAACAACGCGATTTAGCCGTTGGTACAGCCATCGGCTCAAACATTATCa                                                                                                                  <  2:414751/134‑1 (MQ=255)            tGGCATCCCGCCGCTGATCATCGGCATGACGGTGGTCAGTATTGGTACATCGTTACCAGAAGTCATCGTCTCGCTTGCCGCGTCTCTGCACGAACAACGCGATTTAGCCGTTGGTACAGCCATCGGCTCAAACATTATCa                                                                                                                  <  1:208818/140‑1 (MQ=255)            tGGCATCCCGCCGCTGATCATCGGCATGACGGTGGTCAGTATTGGTACATCGTTACCAGAAGTCATCGTCTCGCTTGCCGCGTCTCTGCACGAACAACGCGATTTAGCCGTTGGTACAGCCATCGGCTCAAACATTATCa                                                                                                                  >  2:194914/1‑140 (MQ=255)                  cccGCCGCTGATCATCGGCATGACGGTGGTCAGTATTGGTACATCGTTACCAGAAGTCATCGTCTCGCTTGCCGCGTCTCTGCACGAACAACGCGATTTAGCCGTTGGTACAGCCATCGGCTCAAACATTATCAatatat                                                                                                            >  1:678530/1‑140 (MQ=255)                    cgccgcTGATCATCGGCATGACGGTGGTCAGTATTGGTACATCGTTACCAGAAGTCATCGTCTCGCTTGCCGCGTCTCTGCACGAACAACGCGATTTAGCCGTTGGTACAGCCATCGGCTCAAACATTATCAATATATTg                                                                                                          >  2:631081/1‑140 (MQ=255)                     gccgcTGATCATCGGCATGACGGTGGTCAGTATTGGTACATCGTTACCAGAAGTCATCGTCTCGCTTGCCGCGTCTCTGCACGAACAACGCGATTTAGCCGTTGGTACAGCCATCGGCTCAAACATTATCAATATATTGc                                                                                                         <  1:587668/140‑1 (MQ=255)                      ccgcTGATCATCGGCATGACGGTGGTCAGTATTGGTACATCGTTACCAGAAGTCATCGTCTCGCTTGCCGCGTCTCTGCACGAACAACGCGATTTAGCCGTTGGTACAGCCATCGGCTCAAACATTATCa                                                                                                                  <  2:524799/130‑1 (MQ=255)                      ccgcTGATCATCGGCATGACGGTGGTCAGTATTGGTACATCGTTACCAGAAGTCATCGTCTCGCTTGCCGCGTCTCTGCACGAACAACGCGATTTAGCCGTTGGTACAGCCATCGGCTCAAACATTATCa                                                                                                                  >  1:524799/1‑130 (MQ=255)                          tGATCATCGGCATGACGGTGGTCAGTATTGGTACATCGTTACCAGAAGTCATCGTCTCGCTTGCCGCGTCTCTGCACGAACAACGCGATTTAGCCGTTGGTACAGCCATCGGCTCAAACATTATCAATATATTGCTGATc                                                                                                    <  1:631081/140‑1 (MQ=255)                               atcGGCATGACGGTGGTCAGTATTGGTACATCGTTACCAGAAGTCATCGTCTCGCTTGCCGTGTCTCTGCACGAACAACGCGATTTAGCCGTTGGTACAGCCATCGGCTCAAACATTATCAATATATTGCTGATCCTCgg                                                                                               <  2:148347/140‑1 (MQ=255)                                 cGGCATGACGGTGGTCAGTATTGGTACATCGTTACCAGAAGTCATCGTCTCGCTTGCCGCGTCTCTGCACGAACAACGCGATTTAGCCGTTGGTACAGCCATCGGCTCAAACATTATCAATATATTGCTGATCCTCGGTc                                                                                             >  1:409347/1‑140 (MQ=255)                                      tGACGGTGGTCAGTATTGGTACATCGTTACCAGAAGTCATCGTCTCGCTTGCCGCGTCTCTGCACGAACAACGCGATTTAGCCGTTGGTACAGCCATCGGCTCAAACATTATCAATATATTGCTGATCCTCGGTCTGGCt                                                                                        >  2:440011/1‑140 (MQ=255)                                        aCGGTGGTCAGTATTGGTACATCGTTACCAGAAGTCATCGTCTCGCTTGCCGCGTCTCTGCACGAACAACGCGATTTAGCCGTTGGTACAGCCATCGGCTCAAACATTATCAATATATTGCTGATCCTCGGTCTGGCTgc                                                                                      >  1:604964/1‑140 (MQ=255)                                        aCGGTGGTCAGTATTGGTACATCGTTACCAGAAGTCATCGTCTCGCTTGCCGCGTCTCTGCACGAACAACGCGATTTAGCCGTTGGTACAGCCATCGGCTCAAACATTATCAATATATTGCTGATCCTCGGTCTGGCTgc                                                                                      >  1:65765/1‑140 (MQ=255)                                               tCAGTATTGGTACATCGTTACCAGAAGTCATCGTCTCGCTTGCCGCGTCTCTGCACGAACAACGCGATTTAGCCGTTGGTACAGCCATCGGCTCAAACATTATCAATATATTGCTGATCCTCGGTCTGGCTGCGCTGGtt                                                                               <  1:442388/140‑1 (MQ=255)                                                        gTACATCGTTACCAGAAGTCATCGTCTCGCTTGCCGCGTCTCTGCACGAACAACGCGATTTAGCCGTTGGTACAGCCATCGGCTCAAACATTATCAATATATTGCTGATCCTCGGTCTGGCTGCGCTGGTTCGTCCtttt                                                                      >  1:92984/1‑140 (MQ=255)                                                         tACATCGTTACCAGAAGTCATCGTCTCGCTTGCCGCGTCTCTGCACGAACAACGCGATTTAGCCGTTGGTACAGCCATCGGCTCAAACATTATCAATATATTGCTGATCCTCGGTCTGGCTGCGCTGGTTCGTCCTTTTa                                                                     <  1:190152/140‑1 (MQ=255)                                                             tCGTTACCAGAAGTCATCGTCTCGCTTGCCGCGTCTCTGCACGAACAACGCGATTTAGCCGTTGGTACAGCCATCGGCTCAAACATTATCAATATATTGCTGATCCTCGGTCTGGCTGCGCTGGTTCg                                                                             <  1:652003/128‑1 (MQ=255)                                                             tCGTTACCAGAAGTCATCGTCTCGCTTGCCGCGTCTCTGCACGAACAACGCGATTTAGCCGTTGGTACAGCCATCGGCTCAAACATTATCAATATATTGCTGATCCTCGGTCTGGCTGCGCTGGTTCg                                                                             >  2:652003/1‑128 (MQ=255)                                                             tCGTTACCAGAAGTCATCGTCTCGCTTGCCGCGTCTCTGCACGAACAACGCGATTTAGCCGTTGGTACAGCCATCGGCTCAAACATTATCAATATATTGCTGATCCTCGGTCTGGCTGCGCTGGTTCGTCCTTTTACCGt                                                                 >  1:483114/1‑140 (MQ=255)                                                             tCGTTACCAGAAGTCATCGTCTCGCTTGCCGCGTCTCTGCACGAACAACGCGATTTAGCCGTTGGTACAGCCATCGGCTCAAACATTATCAATATATTGCTGATCCTCGGTCTGGCTGCGCTGGTTCGTCCTTTTACCGt                                                                 <  1:475719/140‑1 (MQ=255)                                                             tCGTTACCAGAAGTCATCGTCTCGCTTGCCGCGTCTCTGCACGAACAACGCGATTTAGCCGTTGGTACAGCCATCGGCTCAAACATTATCAATATATTGCTGATCCTCGGTCTGGCTGCGCTGGTTCGTCCTTTTACCGt                                                                 <  1:142773/140‑1 (MQ=255)                                                             tCGTTACCAGAAGTCATCGTCTCGCTTGCCGCGTCTCTGCACGAACAACGCGATTTAGCCGTTGGTACAGCCATCGGCTCAAACATTATCAATATATTGCTGATCCTCGGTCTGGCTGCGCTGGTTCGTCCTTTTACCGt                                                                 >  1:28311/1‑140 (MQ=255)                                                             tCGTTACCAGAAGTCATCGTCTCGCTTGCCGCGTCTCTGCACGAACAACGCGATTTAGCCGTTGGTACAGCCATCGGCTCAAACATTATCAATATATTGCTGATCCTCGGTCTGGCTGCGCTGGTTCGTCCTTTTACCGt                                                                 >  2:142773/1‑140 (MQ=255)                                                                 tACCAGAAGTCATCGTCTCGCTTGCCGCGTCTCTGCACGAACAACGCGATTTAGCCGTTGGTACAGCCATCGGCTCAAACATTATCAATATATTGCTGATCATCGGTCTGGCTGCGCTGGTTCGTCCTTTTACCGTCCAt                                                             <  2:604964/140‑1 (MQ=255)                                                                     aGAAGTCATCGTCTCGCTTGCCGCGTCTCTGCACGAACAACGCGATTTAGCCGTTGGTACAGCCATCGGCTCAAACATTATCAATATATTGCTGATCCTCGGTCTGGCTGCGCTGGTTCGTCCTTTTACCGTCCATTCTg                                                         <  2:491055/140‑1 (MQ=255)                                                                            aTCGTCTCGCTTGCCGCGTCTCTGCACGAACAACGCGATTTAGCCGTTGGTACAGCCATCGGCTCAAACATTATCAATATATTGCTGATCCTCGGTCTGGCTGCGCTGGTTCGTCCTTTTACCGTCCATTCTGATGTTCt                                                  >  2:385228/1‑140 (MQ=255)                                                                                 ctcGCTTGCCGCGTCTCTGCACGAACAACGCGATTTAGCCGTTGGTACAGCCATCGGCTCAAACATTATCAATATATTGCTGATCCTCGGTCTGGCTGCGCTGGTTCGTCCTTTTACCGTCCATTCTGATGTTCTACGcc                                             <  1:385228/140‑1 (MQ=255)                                                                                 ctcGCTTGCCGCGTCTCTGCACGAACAACGCGATTTAGCCGTTGGTACAGCCATCGGCTCAAACATTATCAATATATTGCTGATCCTCGGTCTGGCTGCGCTGGTTCGTCCTTTTACCGTCCATTCTGATGTTCTACGcc                                             <  1:194914/140‑1 (MQ=255)                                                                                 ctcGCTTGCCGCGTCTCTGCACGAACAACGCGATTTAGCCGTTGGTACAGCCATCGGCTCAAACATTATCAATATATTGCTGATCCTCGGTCTGGCTGCGCTGGTTCGTCCTTTTACCGTCCATTCTGATGTTCTACGcc                                             <  2:196884/140‑1 (MQ=255)                                                                                       tGCCGCGTCTCTGCACGAACAACGCGATTTAGCCGTTGGTACAGCCATCGGCTCAAACATTATCAATATATTGCTGATCCTCGGTCTGGCTGCGCTGGTTCGTCCTTTTACCGTCCATTCTGATGTTCTACGCCGTGAAt                                       <  2:409347/140‑1 (MQ=255)                                                                                       tGCCGCGTCTCTGCACGAACAACGCGATTTAGCCGTTGGTACAGCCATCGGCTCAAACATTATCAATATATTGCTGATCCTCGGTCTGGCTGCGCTGGTTCGTCCTTTTACCGTCCATTCTGATGTTCTACGCCGTGAAt                                       <  1:199527/140‑1 (MQ=255)                                                                                           gcgTCTCTGCACGAACAACGCGATTTAGCCGTTGGTACAGCCATCGGCTCAAACATTATCAATATATTGCTGATCCTCGGTCTGGCTGCGCTGGTTCGTCCTTTTACCGTCCATTCTGATGTTCTACGCCGTGAATTAcc                                   <  2:521065/140‑1 (MQ=255)                                                                                           gcgTCTCTGCACGAACAACGCGATTTAGCCGTTGGTACAGCCATCGGCTCAAACATTATCAATATATTGCTGATCCTCGGTCTGGCTGCGCTGGTTCGTCCTTTTACCGTCCATTCTGATGTTCTACGCCGTGAATTAcc                                   <  1:622601/140‑1 (MQ=255)                                                                                           gcgTCTCTGCACGAACAACGCGATTTAGCCGTTGGTACAGCCATCGGCTCAAACATTATCAATATATTGCTGATCCTCGGTCTGGCTGCGCTGGTTCGTCCTTTTACCGTCCATTCTGATGTTCTACGCCGTGAATTAcc                                   <  2:165950/140‑1 (MQ=255)                                                                                              tctcTGCACGAACAACGCGATTTAGCCGTTGGTACAGCCATCGGCTCAAACATTATCAATATATTGCTGATCCTCGGTCTGGCTGCGCTGGTTCGTCCTTTTACCGTCCATTCTGATGTTCTACGCCGTGAATTACCCtt                                <  2:632473/140‑1 (MQ=255)                                                                                                  tGCACGAACAACGCGATTTAGCCGTTGGTACAGCCATCGGCTCAAACATTATCAATATATTGCTGATCCTCGGTCTGGCTGCGCTGGTTCGTCCTTTTACCGTCCATTCTGATGTTCTACGCCGTGAATTACCCTTAAtg                            >  1:248284/1‑140 (MQ=255)                                                                                                       gAACAACGCGATTTAGCCGTTGGTACAGCCATCGGCTCAAACATTATCAATATATTGCTGATCCTCGGTCTGGCTGCGCTGGTTCGTCCTTTTACCGTCCATTCTGATGTTCTACGCCGTGAATTACCCTTAATgttgtt                       <  2:678530/140‑1 (MQ=255)                                                                                                         acaacGCGATTTAGCCGTTGGTACAGCCATCGGCTCAAACATTATCAATATATTGCTGATCCTCGGTCTGGCTGCGCTGGTTCGTCCTTTTACCGTCCATTCTGATGTTCTACGCCGTGAATTACCCTTAATGTTGTTgg                     >  1:702536/1‑140 (MQ=255)                                                                                                            acGCGATTTAGCCGTTGGTACAGCCATCGGCTCAAACATTATCAATATATTGCTGATCCTCGGTCTGGCTGCGCTGGTTCGTCCTTTTACCGTCCATTCTGATGTTCTACGCCGTGAATTACCCTTAATGTTGTTGGTCa                  >  1:674639/1‑140 (MQ=255)                                                                                                                 aTTTAGCCGTTGGTACAGCCATCGGCTCAAACATTATCAATATATTGCTGATCCTCGGTCTGGCTGCGCTGGTTCGTCCTTTTACCGTCCATTCTGATGTTCTACGCCGTGAATTACCCTTAATGTTGTTGGTCAGCgtg             <  2:577949/140‑1 (MQ=255)                                                                                                                 aTTTAGCCGTTGGTACAGCCATCGGCTCAAACATTATCAATATATTGCTGATCCTCGGTCTGGCTGCGCTGGTTCGTCCTTTTACCGTCCATTCTGATGTTCTACGCCGTGAATTACCCTTAATGTTGTTGGTCAGCgtg             <  1:440011/140‑1 (MQ=255)                                                                                                                     aGCCGTTGGTACAGCCATCGGCTCAAACATTATCAATATATTGCTGATCCTCGGTCTGGCTGCGCTGGTTCGTCCTTTTACCGTCCATTCTGATGTTCTACGCCGTGAATTACCCTTAATGTTGTTGGTCAGCGtggtgg         >  1:247049/1‑140 (MQ=255)                                                                                                                      gCCGTTGGTACAGCCATCGGCTCAAACATTATCAATATATTGCTGATCCTCGGTCTGGCTGCGCTGGTTCGTCCTTTTACCGTCCATTCTGATGTTCTACGCCGTGAATTACCCTTAATGTTGTTGGTCAGCGTGGTGGc        <  1:260194/140‑1 (MQ=255)                                                                                                                      gCCGTTGGTACAGCCATCGGCTCAAACATTATCAATATATTGCTGATCCTCGGTCTGGCTGCGCTGGTTCGTCCTTTTACCGTCCATTCTGATGTTCTACGCCGTGAATTACCCTTAATGTTGTTGGTCAGCGTGGTGGc        <  2:488685/140‑1 (MQ=255)                                                                                                                           tGGTACAGCCATCGGCTCAAACATTATCAATATATTGCTGATCCTCGGTCTGGCTGCGCTGGTTCGTCCTTTTACCGTCCATTCTGATGTTCTACGCCGTGAATTACCCTTAATGTTGTTGGTCAGCGTGGTGGCCGGtt   <  1:425503/140‑1 (MQ=255)                                                                                                                            ggTACAGCCATCGGCTCAAACATTATCAATATATTGCTGATCCTCGGTCTGGCTGCGCTGGTTCGTCCTTTTACCGTCCATTCTGATGTTCTACGCCGTGAATTACCCTTAATGTTGTTGGTCAGCGTGGTGGCCGGTTc  >  1:406626/1‑140 (MQ=255)                                                                                                                                     |                                                                                                                                   TGCCGAACCTTTGGCATCCCGCCGCTGATCATCGGCATGACGGTGGTCAGTATTGGTACATCGTTACCAGAAGTCATCGTCTCGCTTGCCGCGTCTCTGCACGAACAACGCGATTTAGCCGTTGGTACAGCCCTCGGCTCAAACATTATCAATATATTGCTGATCCTCGGTCTGGCTGCGCTGGTTCGTCCTTTTACCGTCCATTCTGATGTTCTACGCCGTGAATTACCCTTAATGTTGTTGGTCAGCGTGGTGGCCGGTTC  >  NC_000913/3340362‑3340624

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 24 ≤ ATCG/ATCG < 31 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑