Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	3,750,633	C→A	A183D (GCC→GAC)	sgbE →	L‑ribulose‑5‑phosphate 4‑epimerase SgbE

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	3,750,633	0	C	A	100.0%	90.0 / NA	28	A183D (GCC→GAC)	sgbE	L‑ribulose‑5‑phosphate 4‑epimerase SgbE
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base A (12/16);  total (12/16)

ATATGAATATCAGACCGGCGAAGTGATCATTGAAACCTTCGAAGAACGTGGCAGGAGTCCGGCACAAATCCCGGCGGTGCTGGTGCATTCTCACGGCCCGTTCGCATGGGGTAAAAACGCCGCCGATGCCGTGCATAACGCCGTAGTACTCGAAGAATGCGCCTATATGGGTCTATTCTCGCGCCAGCTTGCGCCGCAGCTCCCTGCGATGCAAAACGAACTGCTGGATAAGCACTACCTGCGTAAGCATGGGGCCAAT  >  NC_000913/3750505‑3750763                                                                                                                                 |                                                                                                                                   atatgaatatCAGACCGGCGAAGTGATCATTGAAACCTTCGAAGAACGTGGCAGGAGTCCGGCACAAATCCCGGCGGTGCTGGTGCATTCTCACGGCCCGTTCGCATGGGGTAAAAACGCCGCCGATGACGTGCATAACg                                                                                                                         <  1:172788/140‑1 (MQ=255)          tCAGACCGGCGAAGTGATCATTGAAACCTTCGGAGAACGTGGCAGGAGTCCGGCACAAATCCCGGCGGTGCTGGTGCATTCTCACGGCCCGTTCGCATGGGGTAAAAACGCCGCCGATGACGTGCATAACGCCGTAGTAc                                                                                                                >  1:702738/1‑140 (MQ=255)              aCCGGCGAAGTGATCATTGAAACCTTCGAAGAACGTGGCAGGAGTCCGGCACAAATCCCGGCGGTGCTGGTGCATTCTCACGGCCCGTTCGCATGGGGTAAAAACGCCGCCGATGACGTGCATAACGCCGTAGTACTCga                                                                                                            >  1:464115/1‑140 (MQ=255)                  gCGAAGTGATCATTGAAACCTTCGGAGAACGTGGCAGGAGTCCGGCACAAATCCCGGCGGTGCTGGTGCATTCTCACGGCCCGTTCGCATGGGGTAAAAACGCCGCCGATGACGTGCATAACGCCGTAGTACTCgaagaa                                                                                                        <  2:702738/140‑1 (MQ=255)                  gCGAAGTGATCATTGAAACCTTCGAAGAACGTGGCAGGAGTCCGGCACAAATCCCGGCGGTGCTGGTGCATTCTCACGGCCCGTTCGCATGGGGTAAAAACGCCGCCGATGACGTGCATAACGCCGTAGTACTCgaagaa                                                                                                        <  2:91964/140‑1 (MQ=255)                  gCGAAGTGATCATTGAAACCTTCGAAGAACGTGGCAGGAGTCCGGCACAAATCCCGGCGGTGCTGGTGCATTCTCACGGCCCGTTCGCATGGGGGAAAAACGCCGCCGATGACGTGCATAACGCCGTAGTACTCgaagaa                                                                                                        >  2:444563/1‑140 (MQ=255)                  gCGAAGTGATCATTGAAACCTTCGAAGAACGTGGCAGGAGTCCGGCACAAATCCCGGCGGTGCTGGTGCATTCTCACAGCCCGTTCGCCTGGGGTAAAAACGCCGCCGATGACGTGCATAACGCCGTAGTACTCggagaa                                                                                                        >  2:509360/1‑140 (MQ=255)                   cGAAGTGATCATTGAAACCTTCGAAGAACGTGGCAGGAGTCCGGCACAAATCCCGGCGGTGCTGGTGCATTCTCACGGCCCGTTCGCATGGGGTAAAAACGCCGCCGATGACGTGCATAACGCCGTAGTACTCgaagaaa                                                                                                       >  2:56272/1‑139 (MQ=255)                            cATTGAAACCTTCGAAGAACGTGGCAGGAGTCCGGCACAAATCCCGGCGGTGCTGGTGCATTCTCACGGCCCGTTCGCATGGGGTAAAAACGCCGCCGATGACGTGCATAACGCCGTAGTACTCGAAGAATGCGCCtata                                                                                              <  2:117156/140‑1 (MQ=255)                            cATTGAAACCTTCGAAGAACGTGGCAGGAGTCCGGCACAAATCCCGGCGGTGCTGGTGCATTCTCACGGCCCGTTCGCATGGGGTAAAAACGCCGCCGATGACGTGCATAACGCCGTAGTACTCGAAGAATGCGCCtata                                                                                              <  1:182420/140‑1 (MQ=255)                                      ttCGAAGAACGTGGCAGGAGTCCGGCACAAATCCCGGCGGTGCTGGTGCATTCTCACGGCCCGTTCGCATGGGGTAAAAACGCCGCCGATGATGTGCATAACGCCGTAGTACTCGAAGAATGCGCCTATATGGGTCTAtt                                                                                    >  1:594390/1‑140 (MQ=255)                                      ttCGAAGAACGTGGCAGGAGTCCGGCACAAATCCCGGCGGTGCTGGTGCATTCTCACGGCCCGTTCGCATGGGGTAAAAACGCCGCCGATGAAGGGCATAACGCCGTAGTACTCGAAGAATGCGCCTATATGGGTCTAtt                                                                                    >  2:524114/1‑140 (MQ=255)                                               cGTGGCAGGAGTCCGGCACAAATCCCGGCGGTGCTGGTGCATTCTCACGGCCCGTTCGCATGGGGTAAAAACGCCGCCGATGACGTGCATAACGCCGTAGTACTCGAAGAATGCGCCTATATGGGTCTATTCTCGCGCCa                                                                           >  1:676157/1‑140 (MQ=255)                                                 tGGCAGGAGTCCGGCACAAATCCCGGCGGTGCTGGTGCATTCTCACGGCCCGTTCGCATGGGGTAAAAACGCCGCCGATGACGTGCATAACGCCGTAGTACTCGAAGAATGCGCCTATATGGGTCTATTCTCGCGCCAGc                                                                         <  1:245901/140‑1 (MQ=255)                                                    cAGGAGTCCGGCACAAATCCCGGCGGTGCTGGTGCATTCTCACGGCCCGTTCGCATGGGGTAAAAACGCCGCCGATGACGTGCATAACGCCGTAGTACTCGAAGAATGCGCCTATATGGGTCTATTCTCGCGCCAGCTTg                                                                      <  1:509360/140‑1 (MQ=255)                                                           ccGGCACAAATCCCGGCGGTGCTGGTGCATTCTCACGGCCCGTTCGCATGGGGTAAAAACGCCGCCGATGACGTGCATAACGCCGTAGTACTCGAAGAATGCGCCTATATGGGTCTATTCTCGCGCCAGCTTGCGCCGCa                                                               <  2:298493/140‑1 (MQ=255)                                                            cGGCACAAATCCCGGCGGTGCTGGTGCATTCTCACGGCCCGTTCGCATGGGGTAAAAACGCCGCCGATGACGTGCATAACGCCGTAGTACTCGAAGAATGCGCCTATATGGGTCTATTCTCGCGCCAGCTTGCGCCGCAg                                                              <  1:425402/140‑1 (MQ=255)                                                                        cggcggTGCTGGTGCATTCTCACGGCCCGTTCGCATGGGGTAAAAACGCCGCCGATGACGTGCATAACGCCGTAGTACTCGAAGAATGCGCCTATATGGGTCTATTCTCGCGCCAGCTTGCGCCGCAGCTCCCTGCGATg                                                  >  1:59244/1‑140 (MQ=255)                                                                                 tGGTGCATTCTCACGGCCCGTTCGCATGGGGTAAAAACGCCGCCGATGACGTGCATAACGCCGTAGTACTCGAAGAATGCGCCTATATGGGTCTATTCTCGCGCCAGCTTGCGCCGCAGCTCCCTGCGATGCAAAACGaa                                         <  1:326130/140‑1 (MQ=255)                                                                                     gCATTCTCACGGCCCGTTCGCATGGGGTAAAAACGCCGCCGATGACGTGCATAACGCCGTAGTACTCGAAGAATGCGCCTATATGGGTCTATTCTCGCGCCAGCTTGCGCCGCAGCTCCCTGCGATGCAAAACGAActgc                                     <  1:497998/140‑1 (MQ=255)                                                                                      cATTCTCACGGCCCGTTCGCATGGGGTAAAAACGCCGCCGATGACGTGCATAACTCCATAGTACTCGAAGAATGCGCCTATATGGGTCTATTCTCGCGCCAGCTTGCGCGGCAGCTCCCTGCGATGCAAAACGAActgct                                    <  1:703114/140‑1 (MQ=255)                                                                                                 cccGTTCGCATGGGGTAAAAACGCCGCCGATGACGTGCATAACGCCGTAGTACTCGAAGAATGCGCCTATATGGGTCTATTCTCGCGCCAGCTTGCGCCGCAGCTCCCTGCGATGCAAAACGAACTGCTGGATAAGCACt                         >  2:346784/1‑140 (MQ=255)                                                                                                   ctgtCGCATGGGGAAAAAACGCCGCCGATGACGTGCATAACGCCGTAGTACGCGAAGAATGCGCCTATATGGGTCTATTCTCGCGCCCGCTTGCGTCGCAGCGCCCTGCGATGCAAAACGAACTGCTGGATAAGCACTAc                       <  2:489899/137‑1 (MQ=255)                                                                                                   cGTTCGCATGGGGTAAAAACGCCGCCGATGACGTGCATAACGCCGTAGTACTCGAAGAATGCGCCTATATGGGTCTATTCTCGCGCCAGCTTGCGCCGCAGCTCCCTGCGATGCAAAACGAACTGCTGGATAAGCACTAc                       <  2:247/140‑1 (MQ=255)                                                                                                   cGTTCGCATGGGGTAAAAACGCCGCCGATGACGTGCATAACGCCGTAGTACTCGAAGAATGCGCCTATATGGGTCTATTCTCGCGCCAGCTTGCGCCGCAGCTCCCTGCGATGCAAAACGAACTGCTGGATAAGCACTAc                       >  2:535457/1‑140 (MQ=255)                                                                                                   cGTTCGCATGGGGTAAAAACGCCGCCGATGACGTGCATAACGCCGTAGTACTCGAAGAATGCGCCTATATGGGTCTATTCTCGCGCCAGCTTGCGCCGCAGCTCCCTGCGATGCAAAACGAACTGCTGGATAAGCACTAc                       <  1:603748/140‑1 (MQ=255)                                                                                                     ttCGCATGGGGTAAAAACGCCGCCGATGACGTGCATAACGCCGTAGTACTCGAAGAATGCGCCTATATGGGTCTATTCTCGCGCCAGCTTGCGCCGCAGCTCCCTGCGATGCAAAACGAACTGCTGGATAAGCACTACCt                     >  2:222613/1‑140 (MQ=255)                                                                                                     ttCGCATGGGGTAAAAACGCCGCCGATGACGTGCATAACGCCGTAGTACTCGAAGAATGCGCCTATATGGGTCTATTCTCGCGCCAGCTTGCGCCGCAGCTCCCCGCGATGCAAAACGAACTGCTGGATAAGCACTACCt                     >  1:246252/1‑140 (MQ=255)                                                                                                         cATGGGGTAAAAACGCCGCCGATGACGTACATAACGCCGTAGTACTCGAGGAATGCGCCTATATGGGTCTATTCTCGCGCCAGCTTGCGCCGCAGCTCCCTGCGATGCAAAACGAACTGCTGGATAAGCACTACCTGCGt                 <  1:263682/140‑1 (MQ=255)                                                                                                                        ccgccgATGACGTGCATAACGCCGTAGTACTCGAAGAATGCGCCTATATGGGTCTATTCTCGCGCCAGCTTGCGCCGCAGCTCCCTGCGATGCAAAACGAACTGCTGGATAAGCACTACCTGCGTAAGCATGGGGCCAAt  <  1:688212/140‑1 (MQ=255)                                                                                                                                 |                                                                                                                                   ATATGAATATCAGACCGGCGAAGTGATCATTGAAACCTTCGAAGAACGTGGCAGGAGTCCGGCACAAATCCCGGCGGTGCTGGTGCATTCTCACGGCCCGTTCGCATGGGGTAAAAACGCCGCCGATGCCGTGCATAACGCCGTAGTACTCGAAGAATGCGCCTATATGGGTCTATTCTCGCGCCAGCTTGCGCCGCAGCTCCCTGCGATGCAAAACGAACTGCTGGATAAGCACTACCTGCGTAAGCATGGGGCCAAT  >  NC_000913/3750505‑3750763

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 18 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑