Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	877,751	C→A	P95P (CCG→CCT)	rimO ←	ribosomal protein S12 methylthiotransferase RimO

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	877,751	0	C	A	100.0%	148.8 / NA	43	P95P (CCG→CCT)	rimO	ribosomal protein S12 methylthiotransferase RimO
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base A (25/18);  total (25/18)

CGCGGCGTCAGTTTCACACCTTGTTCTGGCACCAGGCTCAGGAATGGGTTGTGTTTCGGTTTTGGCACGTAGTGATGAACGTGCTCCAGAACCTGCTCATAGCTATGAGGCCCGGTGATTTCCAGCACTTTCGGGTGGACTTCGCGGATCTGATCTTCTTTTGCCCCCAGACAACCGGTCACAATAACCTTGCCGTTTTCATTCAACGCTTCACCAATGGCTTCCAGTGATTCTTGTACCGCGCTGTCAATAAAGCCGCAGGTGT  >  NC_000913/877620‑877884                                                                                                                                    |                                                                                                                                      cgcgGCGTCAGTTTCACACCTTGTTCTGGCACCAGGCTCAGGAATGGGTTGTGTTTCGGTTTTGGCACGTAGTGATGAACGTGCTCCAGAACCTGCTCATAGCTATGAGGCCCGGTGATTTCCAGCACTTTAGGGTGGAc                                                                                                                               >  1:143138/1‑140 (MQ=255)  gcggcgTCAGTTTCACACCTTGTTCTGGCACCAGGCTCAGGAATGGGTTGTGTTTCGGTTTTGGCACGTAGTGATGAACGTGCTCCAGAACCTGCTCATAGCTATGAGGCCCGGTGATTTCCAGCACTTTAGGGTGGACt                                                                                                                              >  2:93508/1‑140 (MQ=255)  gcggcgTCAGTTTCACACCTTGTTCTGGCACCAGGCTCAGGAATGGGTTGTGTTTCGGTTTTGGCACGTAGTGATGAACGTGCTCCAGAACCTGCTCATAGCTATGAGGCCCGGTGATTTCCAGCACTTTAGGGTGGACt                                                                                                                              >  2:567147/1‑140 (MQ=255)   cggcgTCAGTTTCACACCTTGTTCTGGCACCAGCCTCAGGAATGGGTTGTGTTTCGGTTTTGGCACGTAGTGATGAACGTGCTCCAGAACCTGCTCATAGCTATGAGGCCCGGTGATTTCCAGCACTTTAGGGTGGACtt                                                                                                                             >  1:432063/1‑140 (MQ=255)                acacCTTGTTCTGGCACCAGGCTCAGGAATGGGTTGTGTTTCGGTTTTGGCACGTAGTGATGAACGTGCTCCAGAACCTGCTCATAGCTATGAGGCCCGGTGATTTCCAGCACTTTAGGGTGGACTTCGCGgatctgatc                                                                                                                >  1:615726/1‑140 (MQ=255)                      tGTTCTGGCACCAGGCTCAGGAATGGGTTGTGTTTCGGTTTTGGCACGTAGTGATGAACGTGCTCCAGAACCTGCTCATAGCTATGAGGCCCGGTGATTTCCAGCACTTTAGGGTGGACTTCGCGGATCTGATCTTCttt                                                                                                          >  2:21360/1‑140 (MQ=255)                      tGTTCTGGCACCAGGCTCAGGAATGGGTTGTGTTTCGGTTTTGGCACGTAGTGATGAACGTGCTCCAGAACCTGCTCATAGCTATGAGGCCCGGTGATTTCCAGCACTTTAGGGTGGACTTCGCGGATCTGATCTTCttt                                                                                                          <  1:567147/140‑1 (MQ=255)                      tGTTCTGGCACCAGGCTCAGGAATGGGGTGTGTTTCGGTTTTGGCACGTAGTGATGAACGTGCTCCAGAACCTGCTCATAGCTATGAGGCCCGGTGATTTCCAGCACTTTAGGGTGGACTTCGCGGATCTGATCTTCttt                                                                                                          >  2:131324/1‑140 (MQ=255)                         tCTGGCACCAGCCTCAGGAATGGGTTGTGTTTCGGTTTTGGCACGTAGTGATGAACGTGCTCCAGAACCTGCTCATAGCTATGAGGCCCGGTGATTTCCAGCACTTTAGGGTGGACTTCGCGGATCTGATCTTCTTTTGc                                                                                                       <  2:432063/140‑1 (MQ=255)                          cTGGCACCAGGCTCAGGAATGGGTTGTGTTTCGGTTTTGGCACGTAGTGATGAACGTGCTCCAGAACCTGCTCATAGCTATGAGGCCCGGTGATTTCCAGCACTTTAGGGTGGACTTCGCGGATCTGATCTTCTTTTGcc                                                                                                      <  1:540889/140‑1 (MQ=255)                                ccAGGCTCAGGAATGGGTTGTGTTTCGGTTTTGGCACGTAGTGATGAACGTGCTCCAGAACCTGCTCATAGCTATGAGGCCCGGTGATTTCCAGCACTTTAGGGTGGACTTCGCGGATCTGATCTTCTTTTGCCCCCAGa                                                                                                >  2:589000/1‑140 (MQ=255)                                   ggCTCAGGAATGGGTTGTGTTTCGGTTTTGGCACGTAGTGATGAACGTGCTCCAGAACCTGCTCATAGCTATGAGGCCCGGTGATTTCCAGCACTTTAGGGTGGACTTCGCGGATCTGATCTTCTTTTGCCCCCAGACaa                                                                                             <  2:549224/140‑1 (MQ=255)                                       cAGGAATGGGTTGTGTTTCGGTTTTGGCACGTAGTGATGAACGTGCTCCAGAACCTGCTCATAGCTATGAGGCCCGGTGATTTCCAGCACTTTAGGGTGGACTTCGCGGATCTGATCTTCTTTTGCCCCCAGACAACCgg                                                                                         <  1:221449/140‑1 (MQ=255)                                       cAGGAATGGGTTGTGTTTCGGTTTTGGCACGTAGTGATGAACGTGCTCCAGAACCTGCTCATAGCTATGAGGCCCGGTGATTTCCAGCACTTTAGGGTGGACTTCGCGGATCTGATCTTCTTTTGCCCCCAGACAACCgg                                                                                         <  1:243935/140‑1 (MQ=255)                                        aGGAATGGGTTGTGTTTCGGTTTTGGCACGTAGTGATGAACGTGCTCCAGAACCTGCTCATAGCTATGAGGCCCGGTGATTTCCAGCACTTTAGGGTGGACTTCGCGGATCTGATCTTCTTTTGCCCCCAGACAACCGGt                                                                                        >  1:577043/1‑140 (MQ=255)                                               ggTTGTGTTTCGGTTTTGGCACGTAGTGATGAACGTGCTCCAGAACCTGCTCATAGCTATGAGGCCCGGTGATTTCCAGCACTTTAGGGTGGACTTCGCGGATCTGATCTTCTTTTGCCCCCAGACAACCGGTCACAATa                                                                                 >  2:31162/1‑140 (MQ=255)                                                  tgtgTTTCGGTTTTGGCACGTAGTGATGAACGTGCTCCAGAACCTGCTCATAGCTATGAGGCCCGGTGATTTCCAGCACTTTAGGGTGGACTTCGCGGATCTGATCTTCTTTTGCCCCCAGACAACCGGTCACAATAAcc                                                                              <  1:105221/140‑1 (MQ=255)                                                    tgtTTCGGTTTTGGCACGTAGTGATGAACGTGCTCCAGAACCTGCTCATAGCTATGAGGCCCGGTGATTTCCAGCACTTTAGGGTGGACTTCGCGGATCTGATCTTCTTTTGCCCCCAGACAACCGGTCACAATAACCtt                                                                            >  2:181782/1‑140 (MQ=255)                                                    tgtTTCGGTTTTGGCACGTAGTGATGAACGTGCTCCAGAACCTGCTCATAGCTATGAGGCCCGGTGATTTCCAGCACTTTAGGGTGGACTTCGCGGATCTGATCTTCTTTTGCCCCCAGACAACCGGTCACAATAACCtt                                                                            >  2:137267/1‑140 (MQ=255)                                                                 gCACGTAGTGATGAACGTGCTCCAGAACCTGCTCATAGCTATGAGGCCCGGTGATTTCCAGCACTTTAGGGTGGACTTCGCGGATCTGATCTTCTTTTGCCCCCAGACAACCGGTCACAATAACCTTGCCGTTttcattc                                                               <  1:82888/140‑1 (MQ=255)                                                                           atgaACGTGCTCCAGAACCTGCTCATAGCTATGAGGCCCGGTGATTTCCAGCACTTTAGGGTGGACTTCGCGGATCTGATCTTCTTTTGCCCCCAGACAACCGGTCACAATAACCTTGCCGTTTTCATTCAACGCTTCAc                                                     >  2:607855/1‑140 (MQ=255)                                                                           atgaACGTGCTCCAGAACCTGCTCATAGCTATGAGGCCCGGTGATTTCCAGCACTTTAGGGTGGACTTCGCGGATCTGATCTTCTTTTGCCCCCAGACAACCGGTCACAATAACCTTGCCGTTTTCATTCAACGCTTCAc                                                     >  1:224535/1‑140 (MQ=255)                                                                             gaACGTGCTCCAGAACCTGCTCATAGCTATGAGGCCCGGTGATTTCCAGCACTTTAGGGTGGACTTCGCGGATCTGATCTTCTTTTGCCCCCAGACAACCGGTCACAATAACCTTGCCGTTTTCATTCAACGCTTCACCa                                                   <  1:137267/140‑1 (MQ=255)                                                                                     tCCAGAACCTGCTCATAGCTATGAGGCCCGGTGATTTCCAGCACTTTAGGGTGGACTTCGCGGATCTGATCTTCTTTTGCCCCCAGACAACCGGTCACAATAACCTTGCCGTTTTCATTCAACGCTTCACCAATGGCTTc                                           <  2:330901/140‑1 (MQ=255)                                                                                         gAACCTGCTCATAGCTATGAGGCCCGGTGATTTCCAGCACTTTAGGGTGGACTTCGCGGATCTGATCTTCTTTTGCCCCCAGACAACCGGTCACAATAACCTTGCCGTTTTCATTCAACGCTTCACCAATGGCTTCCAGt                                       <  1:502551/140‑1 (MQ=255)                                                                                              tGCTCATAGCTATGAGGCCCGGTGATTTCCAGCACTTTAGGGTGGACTTCGCGGATCTGATCTTCTTTTGCCCCCAGACAACCGGTCACAATAACCTTGCCGTTTTCATTCAACGCTTCACCAATGGCTTCCAGTGATTc                                  <  2:392268/140‑1 (MQ=255)                                                                                               gCTCATAGCTATGAGGCCCGGTGATTTCCAGCACTTTAGGGTGGACTTCGCGGATCTGATCTTCTTTTGCCCCCAGACAACCGGTCACAATAACCTTGCCGTTTTCATTCAACGCTTCACCAATGGCTTCCAGTGATTCt                                 >  1:267063/1‑140 (MQ=255)                                                                                                  cATAGCTATGAGGCCCGGTGATTTCCAGCACTTTAGGGTGGACTTCGCGGATCTGATCTTCTTTTGCCCCCAGACAACCGGTCACAATAGCCTTGCCGTTTTCATTCAACGCTTCACCAATGGCTTCCAGTGATTCTTGt                              <  1:93508/140‑1 (MQ=255)                                                                                                        tATGAGGCCCGGTGATTTCCAGCACTTTAGGGTGGACTTCGCGGATCTGATCTTCTTTTGCCCCCAGACAACCGGTCACAATAACCTTGCCGTTTTCATTCAACGCTTCACCAATGGCTTCCAGTGATTCTTGTACcgcg                        <  2:267063/140‑1 (MQ=255)                                                                                                        tATGAGGCCCGGTGATTTCCAGCACTTTAGGGTGGACTTCGCGGATCTGATCTTCTTTTGCCCCCAGACAACCGGTCACAATAACCTTGCCGTTTTCATTCAACGCTTCACCAATGGCTTCCAGTGATTCTTGTACcgcg                        <  2:143138/140‑1 (MQ=255)                                                                                                          tGAGGCCCGGTGATTTCCAGCACTTTAGGGTGGACTTCGCGGATCTGATCTTCTTTTGCCCCCAGACAACCGGTCACAATAACCTTGCCGTTTTCATTCAACGCTTCACCAATGGCTTCCAGTGATTCTTGTACcgcg                        >  2:531562/1‑138 (MQ=255)                                                                                                          tGAGGCCCGGTGATTTCCAGCACTTTAGGGTGGACTTCGCGGATCTGATCTTCTTTTGCCCCCAGACAACCGGTCACAATAACCTTGCCGTTTTCATTCAACGCTTCACCAATGGCTTCCAGTGATTCTTGTACcgcg                        <  1:531562/138‑1 (MQ=255)                                                                                                          tGAGGCCCGGTGATTTCCAGCACTTTAGGGTGGACTTCGCGGATCTGATCTTCTTTTGCCCCCAGACAACCGGTCACAATAACCTTGCCGTTTTCATTCAACGCTTCACCAATGGCTTCCAGTGATTCTTGTACCGCGCt                      >  2:341263/1‑140 (MQ=255)                                                                                                               cccGGTGATTTCCAGCGCTTTAGGGTGGACTTCGCGGATCTGATCTTCTTTTGCCCCCAGACAACCGGTCACAATAACCTTGCCGTTTTCATTCAACGCTTCACCAATGGCTTCCAGTGATTCTTGTACCGCGCTGTCaa                 >  2:622598/1‑140 (MQ=255)                                                                                                               cccGGTGATTTCCAGCACTTTAGGGTGGACTTCGCGGATCTGATCTTCTTTTGCCCCCAGACAACCGGTCACAATAACCTTGCCGTTTTCATTCAACGCTTCACCAATGGCTTCCAGTGATTCTTGTACCGCGCTGTCaa                 >  1:89063/1‑140 (MQ=255)                                                                                                               cccGGTGATTTCCAGCACTTTAGGGTGGACTTCGCGGATCTGATCTTCTTTTGCCCCCAGACAACCGGTCACAATAACCTTGCCGTTTTCATTCAACGCTTCACCAATGGCTTCCAGTGATTCTTGTACCGCGCTGTCaa                 >  2:623270/1‑140 (MQ=255)                                                                                                               cccGGTGATTTCCAGCACTTTAGGGTGGACTTCGCGGATCTGATCTTCTTTTGCCCCCAGACAACCGGTCACAATAACCTTGCCGTTTTCATTCAACGCTTCACCAATGGCTTCCAGTGATTCTTGTACCGCGCTGTCaa                 >  1:492105/1‑140 (MQ=255)                                                                                                               cccGGTGATTTCCAGCACTTTAGGGTGGACTTCGCGGATCTGATCTTCTTTTGCCCCCAGACAACCGGTCACAATAACCTTGCCGTTTTCATTCAACGCTTCACCAATGGCTTCCAGTGACTCTTGTACCGCGCTGTCaa                 >  2:399712/1‑140 (MQ=255)                                                                                                                 cGGTGATTTCCAGCACTTTAGGGTGGACTTCGCGGATCTGATCTTCTTTTGCCCCCAGACAACCGGTCACAATAACCTTGCCGTTTTCATTCAACGCTTCACCAATGGCTTCCAGTGATTCTTGTACCGCGCTGTCAATa               >  1:526110/1‑140 (MQ=255)                                                                                                                 cGGTGATTTCCAGCACTTTAGGGTGGACTTCGCGGATCTGATCTTCTTTTGCCCCCAGACAACCGGTCACAATAACCTTGCCGTTTTCATTCAACGCTTCACCAATGGCTTCCAGTGATTCTTGTACCGCGCTGTCAATa               >  1:3558/1‑140 (MQ=255)                                                                                                                       tttCCAGCACTTTAGGGTGGACTTCGTGGATCTGATCTTCTTTTGCCCCCAGACAACCGGTCACAATAACCTTGCCGTTTTCATTCAACGCTTCACCAATGGCTTCCAGTGATTCTTGTACCGCGCTGTCAATAAAGCCg         <  1:592029/140‑1 (MQ=255)                                                                                                                              cACTTTAGGGTGGACTTCGCGGATCTGATCTTCTTTTGCCCCCAGACAACCGGTCACAATAACCTTGCCGTTTTCATTCAACGCTTCACCAATGGCTTCCAGTGATTCTTGTACCGCGCTGTCAATAAAGCCGCAGgtgt  <  2:3558/140‑1 (MQ=255)                                                                                                                              cACTTTAGGGTGGACTTCGCGGATCTGATCTTCTTTTGCCCCCAGACAACCGGTCACAATAACCTTGCCGTTTTCATTCAACGCTTCACCAATGGCTTCCAGTGATTCTTGTACCGCGCTGTCAATAAAGCCGCAGgtgt  >  2:309456/1‑140 (MQ=255)                                                                                                                                    |                                                                                                                                      CGCGGCGTCAGTTTCACACCTTGTTCTGGCACCAGGCTCAGGAATGGGTTGTGTTTCGGTTTTGGCACGTAGTGATGAACGTGCTCCAGAACCTGCTCATAGCTATGAGGCCCGGTGATTTCCAGCACTTTCGGGTGGACTTCGCGGATCTGATCTTCTTTTGCCCCCAGACAACCGGTCACAATAACCTTGCCGTTTTCATTCAACGCTTCACCAATGGCTTCCAGTGATTCTTGTACCGCGCTGTCAATAAAGCCGCAGGTGT  >  NC_000913/877620‑877884

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑