Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	4,572,231	G→T	intergenic (+316/‑183)	yjiS → / → yjiT	DUF1127 domain‑containing protein YjiS/putative uncharacterized protein YjiT

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	4,572,231	0	G	T	100.0%	219.9 / NA	64	intergenic (+316/‑183)	yjiS/yjiT	DUF1127 domain‑containing protein YjiS/putative uncharacterized protein YjiT
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base T (26/38);  total (26/38)

TTTACAACTCTCATTATTCGCTATTGTGCAGTTTCTCTAATTGTTTTATACCCTGGAAAGTTAAATGTCAGCTACTGAATACTTTTTGATTGTTTGAGATTTATTTTCATTTGAAATTATAAAATCAGGTGATAAATGAGTTGTGATTAATAGATGGTTGATATCATTTTTATCTAAAATTGATTTATAGTATCGACCTGAAAAAATAGTTGTTGCCGCCTGAGTAACTATACAATATTCTGAAAGGTTTTCTTTCAAATTAGAAA  >  NC_000913/4572101‑4572366                                                                                                                                   |                                                                                                                                        tttACAACTCTCATTATTCGCTATTGTGCAGTTTCTCTAATTGTTTTATACCCTGGAAAGTTAAATGTCAGCTACTGAATACTTTTTGATTGTTTGAGATTTATTTTCATTTGAAATTATAAATTCAGGTTATAAATGAg                                                                                                                                <  2:119306/140‑1 (MQ=255) tttACAACTCTCATTATTCGCTATTGTGCAGTTTCTCTAATTGTTTTATACCCTGGAAAGTTAAATGTCAGCTACTGAATACTTTTTGATTGTTTGAGATTTATTTTCATTTGAAATTATAAAATCAGGTTATAAATGAg                                                                                                                                <  1:655600/140‑1 (MQ=255) tttACAACTCTCATTATTCGCTATTGTGCAGTTTCTCTAATTGTTTTATACCCTGGAAAGTTAAATGTCAGCTACTGAATACTTTTTGATTGTTTGAGATTTATTTTCATTTGAAATTATAAAATCAGGTTATAAATGAg                                                                                                                                <  1:585564/140‑1 (MQ=255) tttACAACTCTCATTATTCGCTATTGTGCAGTTTCTCTAATTGTTTTATACCCTGGAAAGTTAAATGTCAGCTACTGAATACTTTTTGATTGTTTGAGATTTATTTTCATTTGAAATTATAAAATCAGGTTATAAATGAg                                                                                                                                <  1:667462/140‑1 (MQ=255) tttACAACTCTCATTATTCGCTATTGTGCAGTTTCTCTAATTGTTTTATACCCTGGAAAGTTAAATGTCAGCTACTGAATACTTTTTGATTGTTTGAGATTTATTTTCATTTGAAATTATAAAATCAGGTTATAAATGAg                                                                                                                                <  1:522804/140‑1 (MQ=255)   tACAACTCTCATTATTCGCTATTGTGCAGTTTCTCTAATTGTTTTATACCCTGGAAAGTTAAATGTCAGCTACTGAATACTTTTTGATTGTTTGAGATTTATTTTCATTTGAAATTATAAAATCAGGTTATAAATGAGtt                                                                                                                              <  2:166481/140‑1 (MQ=255)    aCAACTCTCATTATTCGCTATTGTGCAGTTTCTCTAATTGTTTTATACCCTGGAAAGTTAAATGTCAGCTACTGAATACTTTTTGATTGTTTGAGATTTATTTTCATTTGAAATTATAAAATCAGGTTATAAATGAGTtg                                                                                                                             <  1:86470/140‑1 (MQ=255)          ctcATTATTCGCTATTGTGCAGTTTCTCTAATTGTTTTATACCCTGGAAAGTTAAATGTCAGCTACTGAATACTTTTTGATTGTTTGAGATTTATTTTCATTTGAAATTATAAAATCAGGTTATAAATGAGTTGTGATTa                                                                                                                       >  1:242143/1‑140 (MQ=255)           tcATTATTCGCTATTGTGCAGTTTCTCTAATTGTTTTATACCCTGGAAAGTTAAATGTCAGCTACTGAATACTTTTTGATTGTTTGAGATTTATTTTCATTTGAAATTATAAAATCAGGTTATAAATGAGTTGTGATTaa                                                                                                                      >  1:205093/1‑140 (MQ=255)           tcATTATTCGCTATTGTGCAGTTTCTCTAATTGTTTTATACCCTGGAAAGTTAAATGTCAGCTACTGAATACTTTTTGATTGTTTGAGATTTATTTTCATTTGAAATTATAAAATCAGGTTATAAATGAGTTGTGATTaa                                                                                                                      >  1:622220/1‑140 (MQ=255)           tcATTATTCGCTATTGTGCAGTTTCTCTAATTGTTTTATACCCTGGAAAGTTAAATGTCAGCTACTGAATACTTTTTGATTGTTTGAGATTTATTTTCATTTGAAATTATAAAATCAGGTTATAAATGAGTTGTGATTaa                                                                                                                      >  1:252746/1‑140 (MQ=255)            cATTATTCGCTATTGTGCAGTTTCTCTAATTGTTTTATACCCTGGAAAGTTAAATGTCAGCTACTGAATACTTTTTGATTGTTTGAGATTTATTTTCATTTGAAATTATAAAATCAGGTTATAAATGAGTTGTGATTAAt                                                                                                                     <  1:331428/140‑1 (MQ=255)               tattCGCTATTGTGCAGTTTCTCTAATTGTTTTATACCCTGGAAAGTTAAATGTCAGCTACTGAATACTTTTTGATTGTTTGAGATTTATTTTCATTTGAAATTATAAAATCAGGTTATAAATGAGTTGTGATTAATAGa                                                                                                                  <  2:584448/140‑1 (MQ=255)               tattCGCTATTGTGCAGTTTCTCTAATTGTTTTATACCCTGGAAAGTTAAATGTCAGCTACTGAATACTTTTTGATTGTTTGAGATTTATTTTCATTTGAAATTATAAAATCAGGTTATAAATGAGTTGTGATTAATAGa                                                                                                                  <  1:584435/140‑1 (MQ=255)                   cGCTATTGTGCAGTTTCTCTAATTGTTTTATACCCTGGAAAGTTAAATGTCAGCTACTGAATACTTTTTGATTGTTTGAGATTTATTTTCATTTGAAATTATAAAATCAGGTTATAAATGAGTTGTGATTAATAGATGGt                                                                                                              <  2:242143/140‑1 (MQ=255)                        ttGTGCAGTTTCTCTAATTGTTTTATACCCTGGAAAGTTAAATGTCAGCTACTGAATACTTTTTGATTGTTTGAGATTTATTTTCATTTGAAATTATAAAATCAGGTTATAAATGAGTTGTGATTAATAGATGGTTGata                                                                                                         >  2:195543/1‑140 (MQ=255)                         tgtgCAGTTTCTCTAATTGTTTTATACCCTGGAAAGTTAAATGTCAGCTACTGAATACTTTTTGATTGTTTGAGATTTATTTTCATTTGAAATTATAAAATCAGGTTATAAATGAGTTGTGATTAATAGATGGTTGatat                                                                                                        <  1:388206/140‑1 (MQ=255)                           tgCAGTTTCTCTAATTGTTTTATACCCTGGAAAGTTAAATGTCAGCTACTGAATACTTTTTGATTGTTTGAGATTTATTTTCATTTGAAATTATAAAATCAGGTTATAAATGAGTTGTGATTAATAGATGGTTGATATCa                                                                                                      >  2:542502/1‑140 (MQ=255)                           tgCAGTTTCTCTAATTGTTTTATACCCTGGAAAGTTAAATGTCAGCTACTGAATACTTTTTGATTGTTTGAGATTTATTTTCATTTGAAATTATAAAATCAGGTTATAAATGAGTTGTGATTAATAGATGGTTGATATCa                                                                                                      >  1:32024/1‑140 (MQ=255)                           tgCAGTTTCTCTAATTGTTTTATACCCTGGAAAGTTAAATGTCAGCTACTGAATACTTTTTGATTGTTTGAGATTTATTTTCATTTGAAATTATAAAATCAGGTTATAAATGAGTTGTGATTAATAGATGGTTGATATCa                                                                                                      >  1:624983/1‑140 (MQ=255)                           tgCAGTTTCTCTAATTGTTTTATACCCCGGAAAGTTAAATGTCAGCTACTGAATACTTTTTGATTGTTTGAGATTTATTTTCATTTGAAATTATAAAATCAGGTTATAAATGAGTTGTGATTAATAGATGGTTGATATCa                                                                                                      >  1:226877/1‑140 (MQ=255)                             cAGTTTCTCTAATTGTTTTATACCCTGGAAAGTTAAATGTCAGCTACTGAATACTTTTTGATTGTTTGAGATTTATTTTCATTTGAAATTATAAAATCAGGTTATAAATGAGTTGTGATTAATAGATGGTTGATATCAtt                                                                                                    >  1:581065/1‑140 (MQ=255)                              aGTTTCTCTAATTGTTTTATACCCTGGAAAGTTAAATGTCAGCTACTGAATACTTTTTGATTGTTTGAGATTTATTTTCATTTGAAATTATAAAATCAGGTTATAAATGAGTTGTGATTAATAGATGGTTGATATCAttt                                                                                                   <  2:292220/140‑1 (MQ=255)                                tttCTCTAATTGTTTTATACCCTGGAAAGTTAAATGTCAGCTACTGAATACTTTTTGATTGTTTGAGATTTATTTTCATTTGAAATTATAAAATCAGGTTATAAATGAGTTGTGATTAATAGATGGTTGATATCAttttt                                                                                                 >  2:622303/1‑140 (MQ=255)                                  tctcTAATTGTTTTATACCCTGGAAAGTTAAATGTCAGCTACTGAATACTTTTTGATTGTTTGAGATTTATTTTCATTTGAAATTATAAAATCAGGTTATAAATGAGTTGTGATTAATAGATGGTTGATATCATTTTTAt                                                                                               <  2:236653/140‑1 (MQ=255)                                   ctctAATTGTTTTATACCCTGGAAAGTTAAATGTCAGCTACTGAATACTTTTTGATTGTTTGAGATTTATTTTCATTTGAAATTATAAAATCAGGTTATAAATGAGTTGTGATTAATAGATGGTTGATATCAttt                                                                                                   <  1:666252/135‑1 (MQ=255)                                   ctctAATTGTTTTATACCCTGGAAAGTTAAATGTCAGCTACTGAATACTTTTTGATTGTTTGAGATTTATTTTCATTTGAAATTATAAAATCAGGTTATAAATGAGTTGTGATTAATAGATGGTTGATATCAttt                                                                                                   >  2:666252/1‑135 (MQ=255)                                        aTTGTTTTATACCCTGGAAAGTTAAATGTCAGCTACTGAATTCTTTTTGATTGTTTGAGATTTATTTTCATTTGAAATTATAAAATCAGGTTATAAATGAGTTGTGATTAATAGATGGTTGATATCATTTTTATCTaaaa                                                                                         <  2:620064/140‑1 (MQ=255)                                            ttttATACCCTGGAAAGTTAAATGTCAGCTACTGAATACTTTTTGATTGTTTGAGATTTATTTTCATTTGAAATTATAAAATCAGGTTATAAATGAGTTGTGATTAATAGATGGTTGATATCATTTTTATCTAAAATTGa                                                                                     >  2:176035/1‑140 (MQ=255)                                             tttATACCCTGGAAAGTTAAATGTCAGCTACTGAATACTTTTTGATTGTTTGAGATTTATTTTCATTTGAAATTATAAAATCAGGTTATAAATGAGTTGTGATTAATAGATGGTTGATATCATTTTTATCTAAAATTGAt                                                                                    <  2:499287/140‑1 (MQ=255)                                              ttATACCCTGGAAAGTTAAATGTCAGCTACTGAATACTTTTTGATTGTTTGAGATTTATTTTCATTTGAAATTATAAAATCAGGTTATAAATGAGTTGTGATTAATAGATGGTTGATATCATTTTTATCTAAAATTGAtt                                                                                   >  2:167102/1‑140 (MQ=255)                                                ataCCCTGGAAAGTTAAATGTCAGCTACTGAATACTTTTTGATTGTTTGAGATTTATTTTCATTTGAAATTATAAAATCAGGTTATAAATGAGTTGTGATTAATAGATGGTTGATATCATTTTTATCTAAAATTGATTta                                                                                 <  2:551510/140‑1 (MQ=255)                                                ataCCCTGGAAAGTTAAATGTCAGCTACTGAATACTTTTTGATTGTTTGAGATTTATTTTCATTTGAAATTATAAAATCAGGTTATAAATGAGTTGTGATTAATAGATGGTTGATATCATTTTTATCTAAAATTGATTta                                                                                 <  2:574266/140‑1 (MQ=255)                                                   cccTGGAAAGTTAAATGTCAGCTACTGAATACTTTTTGATTGTTTGAGATTTATTTTCATTTGAAATTATAAAATCAGGTTATAAATGAGTTGTGATTAATAGATGGTTGATATCATTTTTATCTAAAATTGATTTATAg                                                                              >  2:16797/1‑140 (MQ=255)                                                      tGGAAAGTTAAATGTCAGCTACTGAATACTTTTTGATTGTTTGAGATTTATTTTCATTTGAAATTATAAAATCAGGTTATAAATGAGTTGTGATTAATAGATGGTTGATATCATTTTTATCTAAAATTGATTTATAGTAt                                                                           >  2:490576/1‑140 (MQ=255)                                                               aaaTGTCAGCTACTGAATACTTTTTGATTGTTTGAGATTTATTTTCATTTGAAATTATAAAATCAGGTTATAAATGAGTTGTGATTAATAGATGGTTGATATCATTTTTATCTAAAATTGATTTATAGTATCGACCTGaa                                                                  <  2:499909/140‑1 (MQ=255)                                                                aaTGTCAGCTACTGAATACTTTTTGATTGTTTGAGATTTATTTTCATTTGAAATTATAAAATCAGGTTATAAATGAGTTGTGATTAATAGATGGTTGATATCATTTTTATCTAAAATTGATTTATAGTATCGATCTGaaa                                                                 >  2:493449/1‑140 (MQ=255)                                                                aaTGTCAGCTACTGAATACTTTTTGATTGTTTGAGATTTATTTTCATTTGAAATTATAAAATCAGGTTATAAATGAGTTGTGATTAATAGATGGTTGATATCATTTTTATCTAAAATTGATTTATAGTATCGACCTGaaa                                                                 >  2:252125/1‑140 (MQ=255)                                                                      aGCTACTGAATACTTTTTGATTGTTTGAGATTTATTTTCATTTGAAATTATAAAATCAGGTTATAAATGAGTTGTGATTAATAGATGGTTGATATCATTTTTATCTAAAATTGATTTATAGTATCGACCTGAAAAAATAg                                                           <  1:163280/140‑1 (MQ=255)                                                                              aaTACTTTTTGATTGTTTGAGATTTATTTTCATTTGAAATTATAAAATCAGGTTATAAATGAGTTGTGATTAATAGATGGTTGATATCATTTTTATCTAAAATTGATTTATAGTATCGACCTGAAAAAATAGTTGTTgcc                                                   <  1:397597/140‑1 (MQ=255)                                                                              aaTACTTTTTGATTGTTTGAGATTTATTTTCATTTGAAATTATAAAATCAGGTTATAAATGAGTTGTGATTAATAGATGGTTGATATCATTTTTATCTAAAATTGATTTATAGTATCGACCTGAAAAAATAGTTGTTgcc                                                   <  2:343404/140‑1 (MQ=255)                                                                              aaTACTTTTTGATTGTTTGAGATTTATTTTCATTTGAAATTATAAAATCAGGTTATAAATGAGTTGTGATTAATAGATGGTTGATATCATTTTTATCTAAAATTGATTTATACTATCGACCTGAAAAAATAGTTGTTgcc                                                   <  2:44466/140‑1 (MQ=255)                                                                                tACTTTTTGATTGTTTGAGATTTATTTTCATTTGAAATTATAAAATCAGGTTATAAATGAGTTGTGATTAATAGATGGTTGATATCATTTTTATCTAAAATTGATTTATAGTATCGACCTGAAAAAATAGTTGTTgccgc                                                 <  2:205093/140‑1 (MQ=255)                                                                                   tttttGATTGTTTGAGATTTATTTTCATTTGAAATTATAAAATCAGGTTATAAATGAGTTGTGATTAATAGATGGTTGATATCATTTTTATCTAAAATTGATTTATAGTATCGACCTGAAAAAATAGTTGTTGCCGCCTg                                              >  2:447230/1‑140 (MQ=255)                                                                                     tttGATTGTTTGAGATTTATTTTTATTTGAAATTATAAAATCAGGTTATAAATGAGTTGTGATTAATAGATGGTTGATATCATTTTTATCTAAAATTGATTTATAGTATCGACCTGAAAAAATAGTTGTTGCCGCCTGAg                                            >  2:536214/1‑140 (MQ=255)                                                                                     tttGATTGTTTGAGATTTATTTTCATTTGAAATTATAAAATCAGGTTATAAATGAGTTGTGATTAATAGATGGTTGATATCATTTTTATCTAAAATTGATTTATAGTATCGACCTGAAAAAATAGTTGTTGCCGCCTGAg                                            >  1:686289/1‑140 (MQ=255)                                                                                     tttGATTGTTTGAGATTTATTTTCATTTGAAATTATAAAATCAGGTTATAAATGAGTTGTGATTAATAGATGGTTGATATCATTTTTATCTAAAATTGATTTATAGTATCGACCTGAAAAAATAGTTGTTGCCGCCTGAg                                            >  1:522573/1‑140 (MQ=255)                                                                                     tttGATTGTTTGAGATTTATTTTCATTTGAAATTATAAAATCAGGTTATAAATGAGTTGTGATTAATAGATGGTTGATATCATTTTTATCTAAAATTGATTTATAGTATCGACCTGAAAAAATAGTTGTTGCCGCCTGAg                                            >  2:529627/1‑140 (MQ=255)                                                                                     tttGATTGTTTGAGATTTATTTTCATTTGAAATTATAAAATCAGGTTATAAATGAGTTGTGATTAATAGATGGTTGATATCATTTTTATCTAAAATTGATTTATAGTATCGACCTGAAAAAATAGTTGTTGCCGCCTGAg                                            <  2:181622/140‑1 (MQ=255)                                                                                      ttGATTGTTTGAGATTTATTTTCATTTGAAATTATAAAATCAGGTTATAAATGAGTTGTGATTAATAGATGGTTGATATCATTTTTATCTAAAATTGATTTATAGTATCGATCTGAAAAAATAGTTGTTGCCGCCTGAGt                                           <  1:493449/140‑1 (MQ=255)                                                                                      ttGATTGTTTGAGATTTATTTTCATTTGAAATTATAAAATCAGGTTATAAATGAGTTGTGATTAATAGATGGTTGATATCATTTTTATCTAAAATTGATTTATAGTATCGACCTGAAAAAATAGTTGTTGCCGCCTGAGt                                           <  1:529627/140‑1 (MQ=255)                                                                                      ttGATTGTTTGAGATTTATTTTCATTTGAAATTATAAAATCAGGTTATAAATGAGTTGTGATTAATAGATGGTTGATATCATTTTTATCTAAAATTGATTTATAGTATCGACCTGAAAAAATAGTTGTTGCCGCCTGAGt                                           <  2:341102/140‑1 (MQ=255)                                                                                      ttGATTGTTTGAGATTTATTTTCATTTGAAATTATAAAATCAGGTTATAAATGAGTTGTGATTAATAGATGGTTGATATCATTTTTATCTAAAATTGATTTATAGTATCGACCTGAAAAAATAGTTGTTGCCGCCTGAGt                                           <  1:325329/140‑1 (MQ=255)                                                                                        gATTGTTTGAGATTTATTTTCATTTGAAATTATAAAATCAGGTTATAAATGAGTTGTGATTAATAGATGGTTGATATCATTTTTATCTAAAATTGATTTATAGTATCGACCTGAAAAAATAGTTGTTGCCGCCTGAGTaa                                         <  2:624983/140‑1 (MQ=255)                                                                                          ttGTTTGAGATTTATTTTCATTTGAAATTATAAAATCAGGTTATAAATGAGTTGTGATTAATAGATGGTTGATATCATTTTTATCTAAAATTGATTTATAGTATCGACCTGAAAAAATAGTTGTTGCCGCCTGAGTAACt                                       <  2:12387/140‑1 (MQ=255)                                                                                          ttGTTTGAGATTTATTTTCATTTGAAATTATAAAATCAGGTTATAAATGAGTTGTGATTAATAGATGGTTGATATCATTTTTATCTAAAATTGATTTATAGTATCGACCTGAAAAAATAGTTGTTGCCGCCTGAGTAACt                                       <  2:356039/140‑1 (MQ=255)                                                                                             tttGAGATTTATTTTCATTTGAAATTATAAAATCAGGTTATAAATGAGTTGTGATTAATAGATGGTTGATATCATTTTTATCTAAAATTGATTTATAGTATCGACCTGAAAAAATAGTTGTTGCCGCCTGAGTAACtata                                    >  2:321652/1‑140 (MQ=255)                                                                                              ttGAGATTTATTTTCATTTGAAATTATAAAATCAGGTTATAAATGAGTTGTGATTAATAGATGGTTGATATCATTTTTATCTAAAATTGATTTATAGTATCGACCTGAAAAAATAGTTGTTGCCGCCTGAGTAACTATAc                                   <  2:419378/140‑1 (MQ=255)                                                                                               tGAGATTTATTTTCATTTGAAATTATAAAATCAGGTTATAAATGAGTTGTGATTAATAGATGGTTGATATCATTTTTATCTAAAATTGATTTATAGTATCGACCTGAAAAAATAGTTGTTGCCGCCTGAGTAACTATACa                                  >  1:61698/1‑140 (MQ=255)                                                                                                       atttTCATTTGAAATTATAAAATCAGGTTATAAATGAGTTGTGATTAATAGATGGTTGATATCATTTTTATCTAAAATTGATTTATAGTATCGACCTGAAAAAATAGTTGTTGCCGCCTGAGTAACTATACAATATTCTg                          <  1:58116/140‑1 (MQ=255)                                                                                                       atttTCATTTGAAATTATAAAATCAGGTTATAAATGAGTTGTGATTAATAGATGGTTGATATCATTTTTATCTAAAATTGATTTATAGTATCGACCTGAAAAAATAGTTGTTGCCGCCTGAGTAACTATACAATATTCTg                          >  2:570495/1‑140 (MQ=255)                                                                                                         tttCATTTGAAATTATAAAATCAGGTTATAAATGAGTTGTGATTAATAGATGGTTGATATCATTTTTATCTAAAATTGATTTATAGTATCGACCTGAAAAAATAGTTGTTGCCGCCTGAGTAACTATACAATATTCTGaa                        <  2:32024/140‑1 (MQ=255)                                                                                                                    aTTATAAAATCAGGTTATAAATGAGTTGTGATTAATAGATGGTTGATATCATTTTTATCTAAAATTGATTTATAGTATCGACCTGAAAAAATAGTTGTTGCCGCCTGAGTAACTATACAATATTCTGAAAGGTtttcttt             <  1:150034/140‑1 (MQ=255)                                                                                                                               aGGTTATAAATGAGTTGTGATTAATAGATGGTTGATATCATTTTTATCTAAAATTGATTTATAGTATCGACCTGAAAAAATAGTTGTTGCCGCCTGAGTAACTATACAATATTCTGAAAGGTTTTCTTTCAAATTAGaaa  <  2:686289/140‑1 (MQ=255)                                                                                                                                   |                                                                                                                                        TTTACAACTCTCATTATTCGCTATTGTGCAGTTTCTCTAATTGTTTTATACCCTGGAAAGTTAAATGTCAGCTACTGAATACTTTTTGATTGTTTGAGATTTATTTTCATTTGAAATTATAAAATCAGGTGATAAATGAGTTGTGATTAATAGATGGTTGATATCATTTTTATCTAAAATTGATTTATAGTATCGACCTGAAAAAATAGTTGTTGCCGCCTGAGTAACTATACAATATTCTGAAAGGTTTTCTTTCAAATTAGAAA  >  NC_000913/4572101‑4572366

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 26 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑