Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	1,287,240	G→T	intergenic (‑4/+4)	rttR ← / ← tyrV	small RNA RttR/tRNA‑Tyr

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,287,240	0	G	T	100.0%	113.5 / NA	34	intergenic (‑4/+4)	rttR/tyrV	small RNA RttR/tRNA‑Tyr
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base T (17/17);  total (17/17)

CCTTCGGGTCGTTGCCTGCGGCAACGCTCTCTCGCTGGCGCTCGAGTCGAACCTTGGTCGAAGCTTCTCATCCTTCCCCGCATGGGCAGAATATTTAATTGCGGATTCGTTGGGAAGTTCAGGGACTTTTGAAAGTGATGGTGGTGGGGGAAGGATTCGAACCTTCGAAGTCGATGACGGCAGATTTACAGTCTGCTCCCTTTGGCCGCTCGGGAACCCCACCAGGGGTAATTCAAATTTTGAGGTAATGCTTGAGATGGTGGTGGGGG  >  NC_000913/1287106‑1287374                                                                                                                                       |                                                                                                                                       ccTTCGGGTCGTTGCCTGCGGCAACGCTCTCTCGCTGGCGCTCGAGTCGAACCTTGGTCGAAGCTTCTCATCCTTCCCCGCATGGGCAGAATATTTAATTGCGGATTCGTTGGGAAGTTCAGGGACTTTTGAAATTGAtg                                                                                                                                   <  2:491664/140‑1 (MQ=255)  cTTCGGGTCGTTGCCTGCGGCAACGCTCTCTCGCTGGCGCTCGAGTCGAACCTTGGTCGAAGCTTCTCATCCTTCCCCGCATGGGCAGAATATTTAATTGCGGATTCGTTGGGAAGTTCAGGGACTTTTGAAATTGAtgg                                                                                                                                  <  2:370843/140‑1 (MQ=255)        gTCGTTGCCTTCGGCAACGCTGACTCGCTGGCGGTCGTGGCCAGCCTTGGTCGAAGCTACGCGTCCGTCCCCGCATGGGCAGAATATTTAATTGCGGATTCGTTGGGAAGTTCAGGGACTTTTGAAATTGAtggtggtgg                                                                                                                            <  2:150904/140‑1 (MQ=14)             tGCCTGCGGCAACGCTCTCTCGCTGGCGCTCGAGTCGAACCTTGGTCGAAGCTTCTCATCCTTCCCCGCATGGGCAGAATATTTAATTGCGGATTCGTTGGGAAGTTCAGGGACTTTTGAAATTGATGGTGGTGGGGGaa                                                                                                                       <  2:460696/140‑1 (MQ=255)              gCCTGCGGCAACGCTCTCTCGCTGGCGCTCGAGTCGAACCTTGGTCGAAGCTTCTCATCCTTCCCCGCATGGGCAGAATATTTAATTGCGGATTCGTTGGGAAGTTCAGGGACTTTTGAAATTGATGGTGGTGGGGGAAg                                                                                                                      <  1:434764/140‑1 (MQ=255)                  gCGGCAACGCTCTCTCGCTGGCGCTCGAGTCGAACCTTGGTCGAAGCTTCTCATCCTTCCCCGCATGGGCAGAATATTTAATTGCGGATTCGTTGGGAAGTTCAGGGACTTTTGAAATTGATGGTGGTGGGGGAAGGAtt                                                                                                                  >  1:667294/1‑140 (MQ=255)                          gCTCTCTCGCTGGCGCTCGAGTCGAACCTTGGTCGAAGCTTCTCATCCTTCCCCGCATGGGCAGAATATTTAATTGCGGATTCGTTGGGAAGTTCAGGGACTTTTGAAATTGATGGTGGTGGGGGAAGGATTCGAACCtt                                                                                                          <  1:190184/140‑1 (MQ=255)                                tcGCTGGCGCTCGAGTCGAACCTTGGTCGAAGCTTCTCATCCTTCCCCGCATGGGCAGAATATTTAATTGCGGATTCGTTGGGAAGTTCAGGGACTTTTGAAATTGATGGTGGTGGGGGAAGGATTCGAACCTTCGAAGt                                                                                                    >  1:633576/1‑140 (MQ=255)                                         cTCGAGTCGAACCTTGGTCGAAGCTTCTCATCCTTCCCCGCATGGGCAGAATATTTAATTGCGGATTCGTTGGGAAGTTCAGGGACTTTTGAAATTGATGGTGGTGGGGGAAGGATTCGAACCTTCGAAGTCGATGACgg                                                                                           <  1:582786/140‑1 (MQ=255)                                                             aaGCTTCTCATCCTTCCCCGCATGGGCAGAATATTTAATTGCGGATTCGTTGGGAAGTTCAGGGACTTTTGAAATTGATGGTGGTGGGGGAAGGATTCGAACCTTCGAAGTCGATGACGGCAGATTTACAGTCTGCTccc                                                                       >  1:517330/1‑140 (MQ=255)                                                                   ctcATCCTTCCCCGCATGGGCAGAATATTTAATTGCGGATTCGTTGGGAAGTTCAGGGACTTTTGAAATTGATGGTGGTGGGGGAAGGATTCGAACCTTCGAAGTCGATGACGGCAGATTTACAGTCTGCTCCCTTTGGc                                                                 <  2:82376/140‑1 (MQ=255)                                                                           tCCCCGCATGGGCAGAATATTTAATTGCGGATTCGTTGGGAAGTTCAGGGACTTTTGAAATTGATGGTGGTGGGGGAAGGATTCGAACCTTCGAAGTCGATGACGGCAGATTTACAGTCTGCTCCCTTTGGCCGCTCggg                                                         >  2:143737/1‑140 (MQ=255)                                                                                gCATGGGCAGAATATTTAATTGCGGATTCGTTGGGAAGTTCAGGGACTTTTGAAATTGATGGTGGTGGGGGAAGGATTCGAACCTTCGAAGTCGATGACGGCAGATTTACAGTCTGCTCCCTTTGGCCGCTCGGGAAccc                                                    >  1:674110/1‑140 (MQ=255)                                                                                     ggCAGAATATTTAATTGCGGATTCGTTGGGAAGTTCAGGGACTTTTGAAATTGATGGTGGTGGGGGAAGGATTCGAACCTTCGAAGTCGATGACGGCAGATTTACAGTCTGCTCCCTTTGGCCGCTCGGGAACCccacca                                               >  1:391257/1‑140 (MQ=255)                                                                                     ggCAGAATATTTAATTGCGGATTCGTTGGGAAGTTCAGGGACTTTTGAAATTGATGGTGGTGGGGGAAGGATTCGAACCTTCGAAGTCGATGACGGCAGATTTACAGTCTGCTCCCTTTGGCCGCTCGGGAACCccacca                                               >  1:701289/1‑140 (MQ=255)                                                                                          aaTATTTAATTGCGGATTCGTTGGGAAGTTCAGGGACTTTTGAAATTGATGGTGGTGGGGGAAGGATTCGAACCTTCGAAGTCGATGACGGCAGATTTACAGTCTGCTCCCTTTGGCCGCTCGGGAACCCCACCAGGGGt                                          >  1:285739/1‑140 (MQ=255)                                                                                               ttAATTGCGGATTCGTTGGGAAGTTCAGGGACTTTTGAAATTGATGGTGGTGGGGGAAGGATTCGAACCTTCGAAGTCGATGACGGCAGATTTACAGTCTGCTCCCTTTGGCCGCTCGGGAACCCCACCAGGGGTAATTc                                     >  2:187654/1‑140 (MQ=255)                                                                                                    tGCGGATTCGTTGGGAAGTTCAGGGACTTTTGAAATTGATGGTGGTGGGGGCAGGATTCGAACCTTCGAAGTCGATGACGGCAGATTTACAGTCTGCTCCCTTTGGCCGCTCGGGAACCCCACCAGGGGTAATTCAAAtt                                >  1:417973/1‑140 (MQ=255)                                                                                                    tGCGGATTCGTTGGGAAGTTCAGGGACTTTTGAAATTGATGGTGGTGGGGGAAGGATTCGAACCTTCGAAGTCGATGACGGCAGATTTACAGTCTGCTCCCTTTGGCCGCTCGGGAACCCCACCAGGGGTAATTCAAAtt                                >  1:156772/1‑140 (MQ=255)                                                                                                    tGCGGATTCGTTGGGAAGTTCAGGGACTTTTGAAATTGATGGTGGTGGGGGAAGGATTCGAACCTTCGAAGTCGATGACGGCAGATTTACAGTCTGCTCCCTTTGGCCGCTCGGGAACCCCACCAGGGGTAATTCAAAtt                                >  1:311668/1‑140 (MQ=255)                                                                                                      cGGATTCGTTGGGAAGTTCAGGGACTTTTGAAATTGATGGTGGTGGGGGAAGGATTCGAACCTTCGAAGTCGATGACGGCAGATTTACAGTCTGCTCCCTTTGGCCGCTCGGGAACCCCACCAGGGGTAATTCAAAtttt                              >  1:60895/1‑140 (MQ=255)                                                                                                      cGGATTCGTTGGGAAGTTCAGGGACTTTTGAAATTGATGGTGGTGGGGGAAGGATTCGAACCTTCGAAGTCGATGACGGCAGATTTACAGTCTGCTCCCTTTGGCCGCTCGGGAACCCCACCAGGGGTAATTCAAAtttt                              >  1:301289/1‑140 (MQ=255)                                                                                                      cGGATTCGTTGGGAAGTTCAGGGACTTTTGAAATTGATGGTGGTGGGGGAAGGATTCGAACCTTCGAAGTCGATGACGGCAGATTTACAGTCTGCTCCCTTTGGCCGCTCGGGAACCCCACCAGGGGTAATTCAAAtttt                              >  2:422605/1‑140 (MQ=255)                                                                                                       ggATTCGTTGGGAAGTTCAGGGACTTTTGAAATTGATGGTGGTGGGGGAAGGATTCGAACCTTCGAAGTCGATGACGGCAGATTTACAGTCTGCTCCCTTTGGCCGCTCGGGAACCCCACCAGGGGTAATTCAAATTTTg                             >  2:228796/1‑140 (MQ=255)                                                                                                        gATTCGTTGGGAAGTTCAGGGACTTTTGAAATTGATGGTGGTGGGGGAAGGATTCGAACCTTCGAAGTCGATGACGGCAGATTTACAGTCTGCTCCCTTTGGCCGCTCGGGAACCCCACCACGGGGAATTGAATTTTTgt                            >  2:338431/1‑139 (MQ=17)                                                                                                           tCGTTGGGAAGTTCAGGGACTTTTGAAATTGATGGTGGTGGGGGAAGGATTCGAACCTTCGAAGTCGATGACGGCAGATTTACAGTCTGCTCCCTTTGGCCGCTCGGGAACCCCACCAGGGGTAATTCAAATTTTGAGGt                         <  1:395717/140‑1 (MQ=255)                                                                                                           tCGTTGGGAAGTTCAGGGACTTTTGAAATTGATGGTGGTGGGGGAAGGATTCGAACCTTCGAAGTCGATGACGGCAGATTTACAGTCTGCTCCCTTTGGCCGCTCGGGAACCCCACCAGGGGTAATTCAAATTTTGAGGt                         <  1:530526/140‑1 (MQ=255)                                                                                                           tCGTTGGGAAGTTCAGGGACTTTTGAAATTGATGGTGGTGGGGGAAGGATTCGAACCTTCGAAGTCGATGACGGCAGATTTACAGTCTGCTCCCTTTGGCCGCTCGGGAACCCCACCAGGGGTAATTCAAATTTTGAGGt                         <  1:424351/140‑1 (MQ=255)                                                                                                            cGTTGGGAAGTTCAGGGACTTTTGAAATTGATGGTGGTGGGGGAAGGATTCGAACCTTCGAAGTCGATGACGGCAGATTTACAGTCTGCTCCCTTTGGCCGCTCGGGAACCCCACCAGGGGTAATTCAAATTTTGAGGTa                        <  2:301289/140‑1 (MQ=255)                                                                                                                 ggAAGTTCAGGGACTTTTGAAATTGATGGTGGTGGGGGAAGGATTCGAACCTTCGAAGTCGATGACGGCAGATTTACAGTCTGCTCCCTTTGGCCGCTCGGGAACCCCACCAGGGGTAATTCAAATTTTGAGGTAATGCt                   <  1:249416/140‑1 (MQ=255)                                                                                                                 ggAAGTTCAGGGACTTTTGAAATTGATGGTGGTGGGGGAAGGATTCGAACCTTCGAAGTCGATGACGGCAGATTTACAGTCTGCTCCCTTTGGCCGCTCGGGAACCCCACCAGGGGTAATTCAAATTTTGAGGTAATGCt                   <  2:674110/140‑1 (MQ=255)                                                                                                                        cAGGGACTTTTGAAATTGATGGTGGTGGGGGAAGGATTCGAACCTTCGAAGTCGATGACGGCAGATTTACAGTCTGCTCCCTTTGGCCGCTCGGGAACCCCACCAGGGGTAATTCAAATTTTGAGGTAATGCTTGAGAtg            <  2:667294/140‑1 (MQ=255)                                                                                                                                  tGAAATTGATGGTGGTGGGGGAAGGATTCGAACCTTCGAAGTCGATGACGGCAGATTTACAGTTTGCTCCCTTTGGCCGCTCGGTAACCCCACCAGGGGTAATTCAAATTTTGAGGTAATGCTTGAGATGGTGGTggggg  <  2:107120/140‑1 (MQ=255)                                                                                                                                  tGAAATTGATGGTGGTGGGGGAAGGATTCGAACCTTCGAAGTCGATGACGGCAGATTTACAGTCTGCTCCCTTTGGCCGCTCGGGAACCCCACCAGGGGTAATTCAAATTTTGAGGTAATGCTTGAGATGGTGGTggggg  <  2:582510/140‑1 (MQ=255)                                                                                                                                       |                                                                                                                                       CCTTCGGGTCGTTGCCTGCGGCAACGCTCTCTCGCTGGCGCTCGAGTCGAACCTTGGTCGAAGCTTCTCATCCTTCCCCGCATGGGCAGAATATTTAATTGCGGATTCGTTGGGAAGTTCAGGGACTTTTGAAAGTGATGGTGGTGGGGGAAGGATTCGAACCTTCGAAGTCGATGACGGCAGATTTACAGTCTGCTCCCTTTGGCCGCTCGGGAACCCCACCAGGGGTAATTCAAATTTTGAGGTAATGCTTGAGATGGTGGTGGGGG  >  NC_000913/1287106‑1287374

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 19 ≤ ATCG/ATCG < 29 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑