Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	1,741,589	C→A	G59G (GGC→GGA)	cfa →	cyclopropane fatty acyl phospholipid synthase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,741,589	0	C	A	100.0%	86.4 / NA	26	G59G (GGC→GGA)	cfa	cyclopropane fatty acyl phospholipid synthase
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base A (12/14);  total (12/14)

GACAACTGGTACCGTATCGCCAACGAATTACTTAGCCGTGCCGGTATAGCCATTAACGGTTCTGCCCCGGCGGATATTCGTGTGAAAAACCCCGATTTTTTTAAACGCGTTCTGCAAGAAGGCTCTTTGGGGTTAGGCGAAAGTTATATGGATGGCTGGTGGGAATGTGACCGACTGGATATGTTTTTTAGCAAAGTCTTACGCGCAGGTCTCGAGAACCAACTCCCCCATCATTTCAAAGACACGCTGCGTATTGCCGGCGCTCGTCTCT  >  NC_000913/1741452‑1741722                                                                                                                                          |                                                                                                                                      gACAACTGGTACCGTATCGCCAACGAATTACTTAGCCGTGCCGGTATAGCCATTAACGGTTCTGCCCCGGCGGATATTCGTGTGAAAAACCCCGATTTTTTTAAACGCGTTCTGCAAGAAGGCTCTTTGGGGTTAGgaga                                                                                                                                     <  2:664201/140‑1 (MQ=255)      cTGGTACCGTATCGCCAACGAATTACTTAGCCGTGCCGGTATAGCCATTAACGGTTCTGCCCCGGCGGATATTCGTGTGAAAAACCCCGATTTTTTTAAACGCGTTCTGCAAGAAGGCTCTTTGGGGTTAGGAGAAAGtt                                                                                                                                <  2:688052/140‑1 (MQ=255)      cTGGTACCGTATCGCCAACGAATTACTTAGCCGTGCCGGTATAGCCATTAACGGTTCTGCCCCGGCGGATATTCGTGTGAAAAACCCCGATTTTTTTAAACGCGTTCTGCAAGAAGGCTCTTTGGGGTTAGGAGAAAGtt                                                                                                                                <  1:549790/140‑1 (MQ=255)        ggTACCGTATCGCCAACGAATTACTTAGCCGTGCCGGTATAGCCATTAACGGTTCTGCCCCGGCGGATATTCGTGTGAAAAACCCCGATTTTTTTAAACGCGTTCTGCAAGAAGGCTCTTTGGGGTTAGGAGAAAGTtat                                                                                                                              >  2:370816/1‑140 (MQ=255)                 tCGCCAACGATTTACTTAGCCGTGCCGGTATAGCCATTAACGGTTCTGCCCCGGCGGATATTCGTGTGAAAAACCCCGATTTTTTTAAACGCGTTCTGCAAGAAGGCTCTTTGGGGTTAGGAGAAAGTTATATGGATGGc                                                                                                                     >  2:413136/1‑140 (MQ=255)                 tCGCCAACGAATTACTTAGCCGTGCCGGTATAGCCATTAACGGTTCTGCCCCGGCGGATATTCGTGTGAAAAACCCCGATTTTTTTAAACGCGTTCTGCAAGAAGGCTCTTTGGGGTTAGGAGAAAGTTATATGGATGGc                                                                                                                     <  1:352348/140‑1 (MQ=255)                 tCGCCAACGAATTACTTAGCCGTGCCGGTATAGCCATTAACGGTTCTGCCCCGGCGGATATTCGTGTGAAAAACCCCGATTTTTTTAAACGCGTTCTGCAAGAAGGCTCTTTGGGGTTAGGAGAAAGTTATATGGATGGc                                                                                                                     <  2:436153/140‑1 (MQ=255)                      aaCGAATTACTTAGCCGTGCCGGTATAGCCATTAACGGTTCTGCCCCGGCGGATATTCGTGTGAAAAACCCCGATTTTTTTAAACGCGTTCTGCAAGAAGGCTCTTTGGGGTTAGGAGAAAGTTATATGGATGGCtggtg                                                                                                                >  1:197791/1‑140 (MQ=255)                        cGAATTACTTAGCCGTGCCGGTATAGCCATTAACGGTTCTGCCCCGGCGGATATTCGTGTGAAAAACCCCGATTTTTTTAAACGCGTTCTGCAAGAAGGCTCTTTGGGGTTAGGAGAAAGTTATATGGATGGCTGGTggg                                                                                                              >  2:284969/1‑140 (MQ=255)                               cTTAGCCGTGCCGGTATAGCCATTAACGGTTCTGCCCCGGCGGATATTCGTGTGAAAAACCCCGATTTTTTTAAACGCGTTCTGCAAGAAGGCTCTTTGGGGTTAGGAGAAAGTTATATGGATGGCTGGTGGGAATGTGa                                                                                                       <  1:437481/140‑1 (MQ=255)                                  aGCCGTGCCGGTATAGCCATTAACGGTTCTGCCCCGGCGGATATTCGTGTGAAAAACCCCGATTTTTTTAAACGCGTTCTGCAAGAAGGCTCTTTGGGGTTAGGAGAAAGTTATATGGATGGCTGGTGGGAATGTGACCg                                                                                                    <  2:197791/140‑1 (MQ=255)                                   gCCGTGCCGGTATAGCCATTAACGGTTCTGCCCCGGCGGATATTCGTGTGAAAAACCCCGATTTTTTTAAACGCGTTCTGCAAGAAGGCTCTTTGGGGTTAGGAGAAAGTTATATGGATGGCTGGTGGGAATGTGACCGa                                                                                                   <  2:317646/140‑1 (MQ=255)                                    ccGTGCCGGTATAGCCATTAACGGTTCTGCCCCGGCGGATATTCGTGTGAAAAACCCCGATTTTTTTAAACGCGTTCTGCAAGAAGGCTCTTTGGGGTTAGGAGAAAGTTATATGGATGGCTGGTGGGAATGTGACCGAc                                                                                                  <  1:177182/140‑1 (MQ=255)                                    ccGTGCCGGTATAGCCATTAACGGTTCTGCCCCGGCGGATATTCGTGTGAAAAACCCCGATTTTTTTAAACGCGTTCTGCAAGAAGGCTCTTTGGGGTTAGGAGAAAGTTATATGGATGGCTGGTGGGAATGTGACCGAc                                                                                                  <  2:225925/140‑1 (MQ=255)                                       tGCCGGTATAGCCATTAACGGTTCTGCCCCGGCGGATATTCGTGTGAAAAACCCCGATTTTTTTAAACGCGTTCTGCAAGAAGGCTCTTTGGGGCTAGGAGAAAGTTATATGGATGGCTGGTGGGAATGTGACCGACTgg                                                                                               >  2:122750/1‑140 (MQ=255)                                              ataGCCATTAACGGTTCTGCCCCGGCGGATATTCGTGTGAAAAACCCCGATTTTTTTAAACGCGTTCTGCAAGAAGGCTCTTTGGGGTTAGGAGAAAGTTATATGGATGGCTGGTGGGAATGTGACCGACTGGATATGtt                                                                                        >  1:388770/1‑140 (MQ=255)                                                       aaCGGTTCTGCCCCGGCGGATATTCGTGTGAAAAACCCCGATTTTTTTAAACGCGTTCTGCAAGAAGGCTCTTTGGGGTTAGGAGAAAGTTATATGGATGGCTGGTGGGAATGTGACCGACTGGATATGTTTTTTAGCaa                                                                               >  1:73962/1‑140 (MQ=255)                                                                 ccccGGCGGATATTCGTGTGAAAAACCCCGATTTTTTTAAACGCGTTCTGCAAGAAGGCTCTTTGGGGTTAGGAGAAAGTTATATGGATGGCTGGTGGGAATGTGACCGACTGGATATGTTTTTTAGCAAAGTCTTAcgc                                                                     >  1:209797/1‑140 (MQ=255)                                                                  cccGGCGGATATTCGTGTGAAAAACCCCGATTTTTTTAAACGCGTTCTGCAAGAAGGCTCTTTGGGGTTAGGAGAAAGTTATATGGATGGCTGGTGGGAATGTGACCGACTGGATATGTTTTTTAGCAAAGTCTTAcgcg                                                                    <  2:317011/140‑1 (MQ=255)                                                                                 tgtgAAAAACCCCGATTTTTTTAAACGCGTTCTGCAAGAAGGCTCTTTGGGGTTAGGAGAAAGTTATATGGATGGCTGGTGGGAATGTGACCGACTGGATATGTTTTTTAGCAAAGTCTTACGCGCAGGTCTCGAGAAcc                                                     >  2:255824/1‑140 (MQ=255)                                                                                       aaaCCCCGATTTTTTTAAACGCGTTCTGCAAGAAGGCTCTTTGGGGTTAGGAGAAAGTTATATGGATGGCTGGTGGGAATGTGACCGACTGGATATGTTTTTTAGCAAAGTCTTACGCGCAGGTCTCGAGAACCAACTcc                                               <  1:605458/140‑1 (MQ=255)                                                                                                           gcgTTCTGCAAGAAGGCTCTTTGGGGTTAGGAGAAAGTTATATGGATGGCTGGTGGGAATGTGACCGACTGGATATGTTTTTTAGCAAAGTCTTACGCGCAGGTCTCGAGAACCAACTCCCCCATCATTTCAAAGACACg                           <  1:413136/140‑1 (MQ=255)                                                                                                                       aagGCTCTTTGGGGTTAGGAGAAAGTTATATGGATGGCTGGTGGGAATGTGACCGACTGGATATGTTTTTTAGCAAAGTCTTACGCGCAGGTCTCGAGAACCAACTCCCCCATCATTTCAAAGACACGCTGCGTATTGcc               >  2:346072/1‑140 (MQ=255)                                                                                                                           ctctTTGGGGTTAGGAGAAAGTTATATGGATGGCTGGTGGGAATGTGACCGACTGGATATGTTTTTTAGCAAAGTCTTACGCGCAGGTCTCGAGAACCAACTCCCCCATCATTTCAAAGACACGCTGCGTATTGCCGgcg           >  2:229592/1‑140 (MQ=255)                                                                                                                           ctctTTGGGGTTAGGAGAAAGTTATATGGATGGCTGGTGGGAATGTGACCGACTGGATATGTTTTTTAGCAAAGGCTTACGCGCAGGTCTCGAGAACCAACTCCCCCATCATTTCAAAGACACGCTGCGTATTGCCGgcg           >  1:98253/1‑140 (MQ=255)                                                                                                                                tGGGGTTAGGAGAAAGTTATATGGATGGCTGGTGGGAATGTGACCGACTGGATATGTTTTTTAGCAAAGTCTTACGCGCAGGTCTCGAGAACCAACTCCCCCATCATTTCAAAGACACGCTGCGTATTGCCGGCGCTCGt      <  2:17456/140‑1 (MQ=255)                                                                                                                                    gTTAGGAGAAAGTTATATGGATGGCTGGTGGGAATGTGACCGACTGGATATGTTTTTTAGCAAAGTCTTACGCGCAGGTCTCGAGAACCAACTCCCCCATCATTTCAAAGACACGCTGCGTATTGCCGGCGCTCGTctct  <  1:638148/140‑1 (MQ=255)                                                                                                                                          |                                                                                                                                      GACAACTGGTACCGTATCGCCAACGAATTACTTAGCCGTGCCGGTATAGCCATTAACGGTTCTGCCCCGGCGGATATTCGTGTGAAAAACCCCGATTTTTTTAAACGCGTTCTGCAAGAAGGCTCTTTGGGGTTAGGCGAAAGTTATATGGATGGCTGGTGGGAATGTGACCGACTGGATATGTTTTTTAGCAAAGTCTTACGCGCAGGTCTCGAGAACCAACTCCCCCATCATTTCAAAGACACGCTGCGTATTGCCGGCGCTCGTCTCT  >  NC_000913/1741452‑1741722

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 24 ≤ ATCG/ATCG < 30 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑