| Predicted mutation | ||||||
|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | annotation | gene | description |
| RA | NC_000913 | 2,425,164 | C→A | A24S (GCG→TCG) | hisM ← | lysine/arginine/ornithine ABC transporter/histidine ABC transporter, membrane subunit HisM |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_000913 | 2,425,164 | 0 | C | A | 97.3% | 122.5 / NA | 37 | A24S (GCG→TCG) | hisM | lysine/arginine/ornithine ABC transporter/histidine ABC transporter, membrane subunit HisM |
| Reads supporting (aligned to +/- strand): ref base C (0/1); new base A (16/20); total (16/21) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00 | |||||||||||
CGTACCGCGAAAAATATAGGTAAATAACCAGATTGGAAACTGGATGTATTTATTACTGGAGACACGACCAATCGCCAGAAACAGCGCCAGGACTCCGCCTATCACTACCGACAAAATAAGCAGCCACAGAGTGATCGCCACACCAGTAAAGCGATAACCGTCGGTCCACAGCAGCGGTTTCCAGTATTCATGTAAGATTTCGATCACAGGTCAGCCCTCTTCACACCCACGGAGTAGCGGCGCTCAAGGAACAGCAGCACACCATTGGAAACA > NC_000913/2425027‑2425299 | cgTACCGCGAAAAATATAGGTAAATAACCAGATTGGAAACTGGATGTATTTATTACTGGAGACACGACCAATCGCCAGAAACAGCGCCAGGACTCCGCCTATCACTACCGACAAAATAAGCAGCCACAGAGTGATCGAca > 1:564779/1‑140 (MQ=255) aaTAACCAGATTGGAAACTGGATGTATTTATTACTGGAGACACGACCAATCGCCAGAGACAGCGCCAGGACTCCGCCTATCACTACCGACAAAATAAGCAGCCACAGAGTGATCGACACACCAGTAAAGCGATAACCGTc > 2:274806/1‑140 (MQ=255) aGATTGGAAACTGGATGTATTTATTACTGGAGACACGACCAATCGCCAGAAACAGCGCCAGGACTCCGCCTATCACTACCGACAAAATAAGCAGCCACAGAGTGATCGACACACCAGTAAAGCGATAACCGTCGGTCcac > 1:466469/1‑140 (MQ=255) gATTGGAAACTGGATGTATTTATTACTGGAGACACGACCAATCGCCAGAAACAGCGCCAGGACTCCGCCTATCACTACCGACAAAATAAGCAGCCACAGAGTGATCGACACACCAGTAAAGCGATAACCGTCGGTCcaca > 1:254905/1‑140 (MQ=255) ggAAACTGGATGTATTTATTACTGGAGACACGACCAATCGCCAGAAACAGCGCCAGGACTCCGCCTATCACTGCCGACAGAATAAGCAGCCACAGAGTGATCGACACACCAGTAAAGCGATAACCGTCGGTCCAcagcag < 1:276302/140‑1 (MQ=255) aaaCTGGATGTATTTATTACTGGAGACACGACCAATCGCCAGAAACAGCGCCAGGACTCCGCCTATCACTACCGACAAAATAAGCAGCCACAGAGTGATCGACACACCAGTAAAGCGATAACCGTCGGTCCACAGCAGCg < 2:570906/140‑1 (MQ=255) accggATGTATTTATTACTGGAGACACGACCAATCGCCAGAAACAGCGCCAGGACTCCGCCTATCACTACCGACAAAATAAGCAGCCACAGAGTGATCGACACACCAGTAAAGCGATAACCGTCGGTCCACAGCAGCGGt > 1:213952/4‑140 (MQ=255) aCTGGATGTATTTATTACTGGAGACACGACCAATCGCCAGAAACAGCGCCAGGACTCCGCCTATCACTACCGACAAAATAAGCAGCCACAGAGTGATCGACACACCAGTAAAGCGATAACCGTCGGTCCACAGCAGCGGt < 2:353450/140‑1 (MQ=255) cTGGATGTATTTATTACTGGAGACACGACCAATCGCCAGAAACAGCGCCAGGACTCCGCCTATCACTACCGACAAAATAAGCAGCCACAGAGTGATCGACACACCAGTAAAGCGATAACCGTCGGTCCACAGCAGCGGtt < 2:248116/140‑1 (MQ=255) tGGATGTATTTATTACTGGAGACACGACCAATCGCCAGAAACAGCGCCAGGACTCCGCCTATCACTACCGACAAAATAAGCAGCCACAGAGTGATCGACACACCAGTAAAGCGATAACCGTCGGTCCACAGCAGCGGttt > 2:431560/1‑140 (MQ=255) tGGATGTATTTATTACTGGAGACACGACCAATCGCCAGAAACAGCGCCAGGACTCCGCCTATCACTACCGACAAAATAAGCAGCCACAGAGTGATCGACACACCAGTAAAGCGATAACCGTCGGTCCACAGCAGCGGttt > 1:198006/1‑140 (MQ=255) gATGTATTTATTACTGGAGACACGACCAATCGCCAGAAACAGCGCCAGGACTCCGCCTATCACTACCGACAAAATAAGCAGCCACAGAGTGATCGACACACCAGTAAAGCGATAACCGTCGGTCCACAGCAGCGGTTTcc > 2:452141/1‑140 (MQ=255) atttattACTGGAGACACGACCAATCGCCAGAAACAGCGCCAGGACTCCGCCTATCACTACCGACAAAATAAGCAGCCACAGAGTGATCGACACACCAGTAAAGCGATAACCGTCGGTCCACAGCAGCGGTTTCCAGTAt < 1:233611/140‑1 (MQ=255) atttattACTGGAGACACGACCAATCGCCAGAAACAGCGCCAGGACTCCGCCTATCACTACCGACAAAATAAGCAGCCACAGAGTGATCGACACACCAGTAAAGCGATAACCGTCGGTCCACAGCAGCGGTTTCCAGTAt < 1:210261/140‑1 (MQ=255) ttaCTGGAGACACGACCAATCGCCAGAAACAGCGCCAGGACTCCGCCTATCACTACCGACAAAATAAGCAGCCACAGAGTGATCGACACACCAGTAAAGCGATAACCGTCGGTCCACAGCAGCGGTTTCCAGTATTCATg > 2:136966/1‑140 (MQ=255) ttaCTGGAGACACGACCAATCGCCAGAAACAACGCCAGGACTCCGCCTATCACTACCGACAAAATAAGCAGCCACAGAGTGATCGCCACACCAGTAAAGCGATAACCGTCGGTCCACAGCAGCGGTTTCCAGTATTCATg < 2:250351/140‑1 (MQ=255) aCTGGAGACACGACCAATCGCCAGAAACAGCGCCAGGACTCCGCCTATCACTACCGACAAAATAAGCAGCCACAGAGTGATCGACACACCAGTAAAGCGATAACCGTCGGTCCACAGCAGCGGCTTCCAGTATTCATGTa > 2:154981/1‑140 (MQ=255) aCCAATCGCCAGAAACAGCGCCAGGACTCCGCCTATCACTACCGACAAAATAAGCAGCCACAGAGTGATCGACACACCAGTAAAGCGATAACCGTCGGTCCACAGCAGCGGTTTCCAGTATTCATGTAAGATTTCGATca < 1:194192/140‑1 (MQ=255) aGAAACAGCGCCAGGACTCCGCCTATCACTACCGACAAAATAAGCAGCCACAGAGTGATCGACACACCAGTAAAGCGATAACCGTCGGTCCACAGCAGCGGTTTCCAGTATTCATGTAAGATTTCGATCACAGGTCAGcc < 1:406928/140‑1 (MQ=255) gAAACAGCGCCAGGACTCCGCCTATCACTACCGACAAAATAAGCAGCCACAGAGTGATCGACACACCAGTAAAGCGATAACCGTCGGTCCACAGCAGCGGTTTCCAGTATTCATGTAAGATTTCGATCACAGGTCAGccc > 2:314478/1‑140 (MQ=255) gcgcCAGGACTCCGCCTATCACTACCGACAAAATAAGCAGCCACAGAGTGATCGATACACCAGTAAAGCGATAACCGTCGGTCCACAGCAGCGGTTTCCAGTATTCATGTAAGATTTCGATCACAGGTCAGCCCTCTTca < 1:475484/140‑1 (MQ=255) cgcCAGGACTCCGCCTATCACTACCGACAAAATAAGCAGCCACAGAGTGATCGACACACCAGTAAAGCGATAACCGTCGGTCCACAGCAGCGGTTTCCAGTATTCATGTAAGATTTCGATCACAGGTCAGCCCTCTTcac < 1:408474/140‑1 (MQ=255) ggACTCCGCCTATCACTACCGACAAAATAAGCAGCCACAGAGTGATCGACACACCAGTAAAGCGATAACCGTCGGTCCACAGCAGCGGTTTCCAGTATTCATGTAAGATTTCGATCACAGGTCAGCCCTCTTCACACCCa < 1:431560/140‑1 (MQ=255) cGCCTATCACTACCGACAAAATAAGCAGACACAGAGTGATCGACACACCAGTAAAGCGATAACCGTCGGTCCACAGCAGCGGTTTCCAGTATTCATGTAAGATTTCGATCACAGGTCAGCCCTCTTCACACCCACGGAGt > 2:259400/1‑140 (MQ=255) tATCACTACCGACAAAATAAGCAGCCACAGAGTGATCGACACACCAGTAAAGCGATAACCGTCGGTCCACAGCAGCGGTTTCCAGTATTCATGTAAGATTTCGATCACAGGTCAGCCCTCTTCACACCCACGGAGTAgcg < 2:483116/140‑1 (MQ=255) aTCACTACCGACAAAATAAGCAGCCACAGAGTGATCGACACACCAGTAAAGCGATAACCGTCGGTCCACAGCAGCGGTTTCCAGTATTCATGTAAGATTTCGATCACAGGTCAGCCCTCTTCACACCCACGGAGTAgcgg > 2:574423/1‑140 (MQ=255) tCACTACCGACAAAATAAGCAGCCACAGAGTGATCGACACACCAGTAAAGCGATAACCGTCGGTCCACAGCAGCGGTTTCCAGTATTCATGTAAGATTTCGATCACAGGTCAGCCCTCTTCACACCCACGGAGTAgcggc < 2:466737/140‑1 (MQ=255) ccGACAAAATAAGCAGCCACAGAGTGATCGACACACCAGTAAAGCGATAACCGTCGGTCCACAGCAGCGGTTTCCAGTATTCATGTAAGATTTCGATCACAGGTCAGCCCTCTTCACACCCACGGAGTAGCGGCGCTCaa > 1:96846/1‑140 (MQ=255) cAAAATAAGCAGCCACAGAGTGATCGACACACCAGTAAAGCGATAACCGTCGGTCCACAGCAGCGGTTTCCAGTATTCATGTAAGATTTCGATCACAGGTCAGCCCTCTTCACACCCACGGAGTAGCGGCGCTCAAGGaa < 1:136966/140‑1 (MQ=255) gcagcCACAGCGTGATCGTCCAACTAGTAAAGCGATAACCGTCGGTCCACAGCCTCGGTTCCCACTATTCATGTAAGATCTCGATCACAAGTCAGCCCACTTCTCACCCGCGGAGTAGCGGCGCTCAAGGAACAgcagca < 2:198006/140‑1 (MQ=255) gcagcCACAGAGTGATCGACACACCAGTAAAGCGATAACCGTCGGTCCACAGCAGCGGTTTCCAGTATTCATGTAAGATTTCGATCACAGGTCAGCCCTCTTCACACCCACGGAGTAGCGGCGCTCAAGGAACAgcagca < 2:269394/140‑1 (MQ=255) gcagcCACAGAGTGATCGACACACCAGTAAAGCGATAACCGTCGGTCCACAGCAGCGGTTTCCAGTATTCATGTAAGATTTCGATCACAGGTCAGCCCTCTTCACACCCACGGAGTAGCGGCGCTCAAGGAACAgcagca < 2:96846/140‑1 (MQ=255) agcCACAGAGTGATCGACACACCAGTAAAGCGATAACCGTCGGTCCACAGCAGCGGTTTCCAGTATTCATGTAAGATTTCGATCACAGGTCAGCCCTCTTCACACCCACGGAGTAGCGGCGCTCAAGGAACAGCAGcaca < 2:45637/140‑1 (MQ=255) agcCACAGAGTGATCGACACACCAGTAAAGCGATAACCGTCGGTCCACAGCAGCGGTTTCCAGTATTCATGTAAGATTTCGATCACAGGTCAGCCCTCTTCACACCCACGGAGTAGCGGCGCTCAAGGAACAGCAGcaca < 2:25631/140‑1 (MQ=255) agcCACAGAGTGATCGACACACCAGTAAAGCGATAACCGTCGGTCCACAGCAGCGGTTTCCAGTATTCATGTAAGATTTCGATCACAGGTCAGCCCTCTTCACACCCACGGAGTAGCGGCGCTCAAGGAACAGCAGcaca < 1:314478/140‑1 (MQ=255) aTCGACACACCAGTAAAGCGATAACCGTCGGTCCACAGCAGCGGTTTCCAGTATTCATGTAAGATTTCGATCACAGGTCAGCCCTCTTCACACCCACGGAGTAGCGGCGCTCAAGGAACAGCAGCACACCATTGGAAACa > 2:254804/1‑140 (MQ=255) aTCGACACACCAGTAAAGCGATAACCGTCGGTCCACAGCAGCGGTTTCCAGTATTCATGTAAGATTTCGATCACAGGTCAGCCCTCTTCACACCCACGGAGTAGCGGCGCTCAAGGAACAGCAGCACACCATTGGAAACa < 2:564779/140‑1 (MQ=255) aTCGACACACCAGTAAAGCGATAACCGTCGGTCCACAGCAGCGGTTTCCAGTATTCATGTAAGATTTCGATCACAGGTCAGCCCTCTTCACACCCACGGAGTAGCGGCGCTCAAGGAACAGCAGCACACCATTGGAAACa > 1:256/1‑140 (MQ=255) | CGTACCGCGAAAAATATAGGTAAATAACCAGATTGGAAACTGGATGTATTTATTACTGGAGACACGACCAATCGCCAGAAACAGCGCCAGGACTCCGCCTATCACTACCGACAAAATAAGCAGCCACAGAGTGATCGCCACACCAGTAAAGCGATAACCGTCGGTCCACAGCAGCGGTTTCCAGTATTCATGTAAGATTTCGATCACAGGTCAGCCCTCTTCACACCCACGGAGTAGCGGCGCTCAAGGAACAGCAGCACACCATTGGAAACA > NC_000913/2425027‑2425299 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 23 ≤ ATCG/ATCG < 29 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |