Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	3,226,578	C→A	F115L (TTC→TTA)	ygjI →	putative transporter YgjI

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	3,226,578	0	C	A	100.0%	143.2 / NA	42	F115L (TTC→TTA)	ygjI	putative transporter YgjI
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base A (15/27);  total (15/27)

CGGTGTCTACGCGTGGGTAAAAAGTTCGCTGGGCGGACGTTGGGCATTTATTACTGCCTATACCTACTGGTTCGTAAACCTGTTC‑TTTTTCACCTCACTGTTGCCGCGCGTTATTGCTTATGCTTCGTATGCCTTCCTCGGATACGAATATATTATGACGCCGGTTGCCACCACCATTATCAGTATGGTGCTGTTCGCCTTCTCCACCTGGGTTTCCACCAACGGGGCGAAAATGTTGGGGCCAATTACCTCCGTCACTTCAACGCTG  >  NC_000913/3226443‑3226710                                                                                                                                         |                                                                                                                                     cGGTGTCTACGCGTGGGTAAAAAGTTCGCTGGGCGGACGTTGGGCATTTATTACTGCCTATACCTACTGGTTCGTAAACCTGTTC‑TTTTTCACCTCACTGTTGCCGCGCGTTATTGCTTATGCTTCGTATGCCTTACTCg                                                                                                                                  <  2:110459/140‑1 (MQ=255) cGGTGTCTACGCGTGGGTAAAAAGTTCGCTGGGCGGACGTTGGGCATTTATTACTGCCTATACCTACTGGTTCGTAAACCTGTTC‑TTTTTCACCTCACTGTTGCCGCGCGTTATTGCTTATGCTTCGTATGCCTTACTCg                                                                                                                                  <  1:350958/140‑1 (MQ=255)  ggTGTCTACGCGTGGGTAAAAAGTTCGCTGGGCGGACGTTGGGCATTTATTACTGCCTATACCTACTGGTTCGTAAACCTGTTC‑TTTTTCACCTCACTGTTGCCGCGCGTTATTGCTTATGCTTCGTATGCCTTACTCgg                                                                                                                                 >  1:60772/1‑140 (MQ=255)   gtgtCTACGCGTGGGTAAAAAGTTCGCTGGGCGGACGTTGGGCATTTATTACTGCCTATACCTACTGGTTCGTAAACCTGTTC‑TTTTTCACCTCACTGTTGCCGCGCGTTATTGCTTATGCTTCGTATGCCTTACTCGGa                                                                                                                                <  2:387103/140‑1 (MQ=255)   gtgtCTACGCGTGGGTAAAAAGTTCGCTGGGCGGACGTTGGGCATTTATTACTGCCTATACCTACTGGTTCGTAAACCTGTTC‑TTTTTCACCTCACTGTTGCCGCGCGTTATTGCTTATGCTTCGTATGCCTTACTCGGa                                                                                                                                <  1:328560/140‑1 (MQ=255)   gtgtCTACGCGTGGGTAAAAAGTTCGCTGGGCGGACGTTGGGCATTTATTACTGCCTATACCTACTGGTTCGTAAACCTGTTC‑TTTTTCACCTCACTGTTGCCGCGCGTTATTGCTTATGCTTCGTATGCCTTACTCGGa                                                                                                                                >  1:418890/1‑140 (MQ=255)    tgtCTACGCGTGGGTAAAAAGTTCGCTGGGCGGACGTTGGGCATTTATTACTGCCTATACCTACTGGTTCGTAAACCTGTTC‑TTTTTCACCTCACTGTTGCCGCGCGTTATTGCTTATGCTTCGTATGCCTTACTCGGAt                                                                                                                               <  2:85598/140‑1 (MQ=255)     gtCTACGCGTGGGTAAAAAGTTCGCTGGGCGGACGTTGGGCATTTATTACTGCCTATACCTACTGGTTCGTAAACCTGTTC‑TTTTTCACCTCACTGTTGCCGCGCGTTATTGCTTATGCTTCGTATGCCTTACTCGGATa                                                                                                                              <  1:305166/140‑1 (MQ=255)      tCTACGCGTGGGTAAAAAGTTCGCTGGGCGGACGTTGGGCATTTATTACTGCCTATACCTACTGGTTCGTAAACCTGTTC‑TTTTTCACCTCACTGTTGCCGCGCGTTATTGCTTATGCTTCGTATGCCTTACTCGGATAc                                                                                                                             <  2:23906/140‑1 (MQ=255)        tACGCGTGGGTAAAAAGTTCGCTGGGCGGACGTTGGGCATTTATTACTGCCTATACCTACTGGTTCGTAAACCTGTTC‑TTTTTCACCTCACTGTTGCCGCGCGTTATTGCTTATGCTTCGTATGCCTTACTCGGATACGa                                                                                                                           <  2:87779/140‑1 (MQ=255)         aCGCGTGGGTAAAAAGTTCGCTGGGCGGACGTTGGGCATTTATTACTGCCTATACCTACTGGTTCGTAAACCTGTTC‑TTTTTCACCTCACTGTTGCCGCGCGTTATTGCTTATGCTTCGTATGCCTTACTCGGATACGaa                                                                                                                          <  1:50257/140‑1 (MQ=255)                ggTAAAAAGTTCGCTGGGCGGACGTTGGGCATTTATTACTGCCTATACCTACTGGTTCGTAAACCTGTTC‑TTTTTCACCTCACTGTTGCCGCGCGTTATTGCTTATGCTTCGTATGCCTTACTCGGATAc                                                                                                                             >  1:451873/1‑130 (MQ=255)                ggTAAAAAGTTCGCTGGGCGGACGTTGGGCATTTATTACTGCCTATACCTACTGGTTCGTAAACCTGTTC‑TTTTTCACCTCACTGTTGCCGCGCGTTATTGCTTATGCTTCGTATGCCTTACTCGGATAc                                                                                                                             <  2:451873/130‑1 (MQ=255)                  tAAAAAGTTCGCTGGGCGGACGTTGGGCATTTATTACTGCCTATACCTACTGGTTCGTAAACCTGTTC‑TTTTTCACCTCACTGTTGCCGCGCGTTATTGCTTATGCTTCGTATGCCTTACTCGGATACGAATATATTATg                                                                                                                 >  1:134482/1‑140 (MQ=255)                               gggCGGACGTTGGGCATTTATTACTGCCTATACCTACTGGTTCGTAAACCTGTTC‑TTTTTCACCTCACTGTTGCCGCGCGTTATTGCTTATGCTTCGTATGCCTTACTCGGATACGAATATATTATGACGCCGGTTGcca                                                                                                    <  2:574218/140‑1 (MQ=255)                               gggCGGACGTTGGGCATTTATTACTGCCTATACCTACTGGTTCGTAAACCTGTTC‑TTTTTCACCTCACTGTTGCCGCGCGTTATTGCTTATGCTTCGTATGCCTTACTCGGATACGAATATATTATGACGCCGGTTGcca                                                                                                    <  2:322352/140‑1 (MQ=255)                               gggCGGACGTTGGGCATTTATTACTGCCTATACCTACTGGTTCGTAAACCTGTTC‑TTTTTCACCTCACTGTTGCCGCGCGTTATTGCTTATGCTTCGTATGCCTTACTCGGATACGAATATATTATGACGCCGGATGcca                                                                                                    <  2:283101/140‑1 (MQ=255)                                 gCGGACGTTGGGCATTTATTACTGCCTATACCTACTGGTTCGTAAACCTGTTC‑TTTTTCACCTCACTGTTGCCGCGCGTTATTGCTTATGCTTCGTATGCCTTACTCGGATACGAATATATTATGACGCCGGTTGccacc                                                                                                  <  1:548556/140‑1 (MQ=255)                                    gACGTTGGGCATTTATTACTGCCTATACCTACTGGTTCGTAAACCTGTTC‑TTTTTCACCTCACTGTTGCCGCGCGTTATTGCTTATGCTTCGTATGCCTTACTCGGATACGAATATATTATGACGCCGGTTGccaccacc                                                                                               <  1:562236/140‑1 (MQ=255)                                         tGGGCATTTATTACTGCCTATACCTACTGGTTCGTAAACCTGTTC‑TTTTTCACCTCACTGTTGCCGCGCGTTATTGCTTATGCTTCGTATGCCTTACTCGGATACGAATATATTATGACGCCGGTTGCCACCACCATTAt                                                                                          <  1:344228/140‑1 (MQ=255)                                         tGGGCATTTATTACTGCCTATACCTACTGGTTCGTAAACCTGTTC‑TTTTTCACCTCACTGTTGCCGCGCGTTATTGCTTATGCTTCGTATGCCTTACTCGGATACGAATATATTATGACGCCGGTTGCCACCACCATTAt                                                                                          <  1:198041/140‑1 (MQ=255)                                         tGGGCATTTATTACTGCCTATACCTACTGGTTCGTAAACCTGTTC‑TTTTTCACCTCACTGTTGCCGCGCGTTATTGCTTATGCTTCGTATGCCTTACTCGGATACGAATATATTATGACGCCGGTTGCCACCACCATTAt                                                                                          <  1:179500/140‑1 (MQ=255)                                          gggCATTTATTACTGCCTATACCTACTGGTTCGTAAACCTGTTCTTTTTTCACCTCACTGTTGCCGCGCGTTATTGCTTATGCTTCGTATGCCTTACTCGGATACGAATATATTATGACGCCGGTTGCCACCACCATTAt                                                                                          <  1:326367/140‑1 (MQ=255)                                          gggCATTTATTACTGCCTATACCTACTGGTTCGTAAACCTGTTC‑TTTTTCACCTCACTGTTGCCGCGCGTTATTGCTTATGCTTCGTATGCCTTACTCGGATACGAATATATTATGACGCCGGTTGccaccacc                                                                                               >  1:413254/1‑134 (MQ=255)                                          gggCATTTATTACTGCCTATACCTACTGGTTCGTAAACCTGTTC‑TTTTTCACCTCACTGTTGCCGCGCGTTATTGCTTATGCTTCGTATGCCTTACTCGGATACGAATATATTATGACGCCGGTTGccaccacc                                                                                               <  2:413254/134‑1 (MQ=255)                                                 tattaCTGCCTATACCTACTGGTTCGTAAACCTGTTC‑TTTTTCACCTCACTGTTGCCGCGCGTTATTGCTTATGCTTCGTATGCCTTACTCGGATACGAATATATTATGACGCCGGTTGCCACCACCATTATCAGTATgg                                                                                  <  2:558485/140‑1 (MQ=255)                                                         ccTATACCTACTGGTTCGTAAACCTGTTC‑TTTTTCACCTCACTGTTGCCGCGCGTTATTGCTTATGCTTCGTATGCCTTACTCGGATACGAATATATTATGACGCCGGTTGCCACCACCATTATCAGTATGGTGCTGTTc                                                                          >  2:128011/1‑140 (MQ=255)                                                          cTATACCTACTGGTTCGTAAACCTGTTC‑TTTTTCACCTCACTGTTGCCGCGCGTTATTGCTTATGCTTCGTATGCCTTACTCGGATACGAATATATTATGACGCCGGTTGCCACCACCATTATCAGTATGGTGCTGTTCg                                                                         <  1:146916/140‑1 (MQ=255)                                                                   cTGGTTCGTAAACCTGTTC‑TTTTTCACCTCACTGTTGCCGCGCGTTATTGCTTATGCTTCGTATGCCTTACTCGGATACGAATATATTATGACGCCGGTTGCCACCACCATTATCAGTATGGTGCTGTTCGCCTTCTCCa                                                                >  1:249244/1‑140 (MQ=255)                                                                        tCGTAAACCTGTTC‑TTTTTCACCTCACTGTTGCCGCGCGTTATTGCTTATGCTTCGTATGCCTTACTCGGATACGAATATATTATGACGCCGGTTGCCACCACCATTATCAGTATGGTGCTGTTCGCCTTCTCCACCTgg                                                           >  1:360251/1‑140 (MQ=255)                                                                                   ttC‑TTTTTCACCTCACTGTTGCCGCGCGTTATTGCTTATGCTTCGTATGCCTTACTCGGATACGAATATATTATGACGCCGGTTGCCACCACCATTATCAGTATGGTGCTGTTCGCCTTCTCCACCTGGGTTTCCACCaa                                                >  2:541415/1‑140 (MQ=255)                                                                                                 cACTGTTGCCGCGCGTTATTGCTTATGCTTCGTATGCCTTACTCGGATACGAATATATTATGACGCCGGTTGCCACCACCATTATCAGTATGGTGCTGTTCGCCTTCTCCACCTGGGTTTCCACCAACGGGGCGAAAATg                                   >  1:155189/1‑140 (MQ=255)                                                                                                  aCTGTTGCCGCGCGTTATTGCTTATGCTTCGTATGCCTTACTCGGATACGAATATATTATGACGCCGGTTGCCACCACCATTATCAGTATGGTGCTGTTCGCCTTCTCCACCTGGGTTTCCACCAACGGGGCGAAAATGt                                  <  1:597112/140‑1 (MQ=255)                                                                                                       tGCCGCGCGTTATTGCTTATGCTTCGTATGCCTTACTCGGATACGAATATATTATGACGCCGGTTGCCACCACCATTATCAGTATGGTGCTGTTCGCCTTCTCCACCTGGGTTTCCACCAACGGGGCGAAAATGTTgggg                             <  1:64349/140‑1 (MQ=255)                                                                                                            cgcgTTATTGCTTATGCTTCGTATGCCTTACTCGGATACG‑‑‑ATATTATGACGCCGGTTGCCACCACCATTATCAGTATGGTGCTGTTCGCCTTCTCCACCTGGGTTTCCACCAACGGGGCGAAAATGTTGGGGCCAATTAc                     >  1:437062/1‑140 (MQ=255)                                                                                                              cgTTATTGCTTATGCTTCGTATGCCTTACTCGGATACGAATATATTATGACGCCGGTTGCCACCACCATTATCAGTATGGTGCTGTTCGCCTTCTCCACCTGGGTTTCCACCAACGGGGCGAAAATGTTGGGGCCAATTa                      >  1:210910/1‑140 (MQ=255)                                                                                                                     gCTTATGCTTCGTATGCCTTACTCGGATACGAATATATTATGACGCCGGTTGCCACCACCATTATCAGTATGGTGCTGTTCGCCTTCTCCACCTGGGTTTCCACCAACGGGGCGAAAATGTTGGGGCCAATTACCTCCGt               <  2:134482/140‑1 (MQ=255)                                                                                                                     gCTTATGCTTCGTATGCCTTACTCGGATACGAATATATTATGACGCCGGTTGCCACCACCATTATCAGTATGGTGCTGTTCGCCTTCTCCACCTGGGTTTCCACCAACGGGGCGAAAATGTTGGGGCCAATTACCTCCGt               <  2:258688/140‑1 (MQ=255)                                                                                                                           gCTTCGTATGCCTTACTCGGATACGAATATATTATGACGCCGGTTGCCACCACCATTATCAGTATGGTGCTGTTCGCCTTCTCCACCTGGGTTTCCACCAACGGGGCGAAAATGTTGGGGCCAATTACCTCCGTCACTTc         >  2:498904/1‑140 (MQ=255)                                                                                                                                gTATGCCTTACTCGGATACGAATATATTATGACGCCGGTTGCCACCACCATTATCAGTATGGTGCTGTTCGCCTTCTCCACCTGGGTTTCCACCAACGGGGCGAAAATGTTGGGGCCAATTACCTCCGTCACTTCAACGc    >  1:338096/1‑140 (MQ=255)                                                                                                                                 tATGCCTTACTCGGATACGAATATATTATGACGCCGGTTGCCACCACCATTATCAGTATGGTGCTGTTCGCCTTCTCCACCTGGGTTTCCACCAACGGGGCGAAAATGTTGGGGCCAATTACCTCCGTCACTTCAACGCt   >  2:219171/1‑140 (MQ=255)                                                                                                                                  aTGCCTTACTCGGATACGAATATATTATGACGCCGGTTGCCACCACCATTATCAGTATGGTGCTGTTCGCCTTCTCCACCTGGGTTTCCACCAACGGGGCGAAAATGTTGGGGCCAATTACCTCCGTCACTTCAACGCTg  <  1:88135/140‑1 (MQ=255)                                                                                                                                         |                                                                                                                                     CGGTGTCTACGCGTGGGTAAAAAGTTCGCTGGGCGGACGTTGGGCATTTATTACTGCCTATACCTACTGGTTCGTAAACCTGTTC‑TTTTTCACCTCACTGTTGCCGCGCGTTATTGCTTATGCTTCGTATGCCTTCCTCGGATACGAATATATTATGACGCCGGTTGCCACCACCATTATCAGTATGGTGCTGTTCGCCTTCTCCACCTGGGTTTCCACCAACGGGGCGAAAATGTTGGGGCCAATTACCTCCGTCACTTCAACGCTG  >  NC_000913/3226443‑3226710

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑