Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	3,335,704	G→T	A264E (GCG→GAG)	murA ←	UDP‑N‑acetylglucosamine 1‑carboxyvinyltransferase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	3,335,704	0	G	T	100.0%	95.6 / NA	28	A264E (GCG→GAG)	murA	UDP‑N‑acetylglucosamine 1‑carboxyvinyltransferase
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base T (17/11);  total (17/11)

GGGCCTGCATATCGGTCGGGAATGCCGGATGCGGCGCGGTACGTACGTTAACAGCCTTCGGACGTTTGCCATGCATATCCAGGCTAATCCAGTCTTCGCCGACTTCGATGTCCGCTCCAGCGTCACGCAGTTTCGCCAGCACGGCGTCGAGAGTATCTGGCTGCGCGTTACGGCAGATAATTTTGCCGCGAGAAATCGCCGCCGCCACCAGGAAAGTACCGGTTTCGATACGATCCGGCAGAACGCGATAGACACCGCCG  >  NC_000913/3335570‑3335829                                                                                                                                       |                                                                                                                              gggCCTGCATATCGGTCGGGAATGCCGGATGCGGCGCGGTACGTACGTTAACAGCCTTCGGACGTTTGCCATGCATATCCAGGCTAATCCAGTCTTCGCCGACTTCGATGTCCGCTCCAGCGTCACGCAGTTTCTCCAGc                                                                                                                          >  2:124725/1‑140 (MQ=255)  ggCCTGCATATCGGTCGGGAATGCCGGATGCGGCGCGGTACGTACGTTAACAGCCTTCGGACGTTTGCCATGCATATCCAGGCTAATCCAGTCTTCGCCGACTTCGATGTCCGCTCCAGCGTCACGCAGTTTCTCCAGCa                                                                                                                         >  1:388452/1‑140 (MQ=255)  ggCCTGCATATCGGTCGGGAATGCCGGATGCGGCGCGGTACGTACGTTAACAGCCTTCGGACGTTTGCCATGCATATCCAGGCTAATCCAGTCTTCGCCGACTTCGATGTCCGCTCCAGCGTCACGCAGTTTCTCCAGCa                                                                                                                         <  2:124549/140‑1 (MQ=255)    ccTGCATATCGGTCGGGAATGCCGGATGCGGCGCGGTACGTACGTTAACAGCCTTCGGACGTTTGCCATGCATATCCAGGCTAATCCAGTCTTCGCCGACTTCGATGTCCGCTCCAGCGTCACGCAGTTTCTCCAGCACg                                                                                                                       <  2:8143/140‑1 (MQ=255)      tGCATATCGGTCGGGAATGCCGGATGCGGCGCGGTACGTACGTTAACAGCCTTCGGACGTTTGCCATGCATATCCAGGCTAATCCAGTCTTCGCCGACTTCGATGTCCGCTCCAGCGTCACGCAGTTTCTCCAGCACGGc                                                                                                                     >  1:491588/1‑140 (MQ=255)       gCATATCGGTCGGGAATGCCGGATGCGGCGCGGTACGTACGTTAACAGCCTTCGGACGTTTGCCATGCATATCCAGGCTAATCCAGTCTTCGCCGACTTCGATGTCCGCTCCAGCGTCACGCAGTTTCTCCAGCACGGCg                                                                                                                    >  2:219519/1‑140 (MQ=255)       gCATATCGGTCGGGAATGCCGGATGCGGCGCGGTACGTACGTTAACAGCCTTCGGACGTTTGCCATGCATATCCAGGCTAATCCAGTCTTCGCCGACTTCGATGTCCGCTCCAGCGTCACGCAGTTTCTCCAGCACGGCg                                                                                                                    >  1:545038/1‑140 (MQ=255)       gCATATCGGTCGGGAATGCCGGATGCGGCGCGGTACGTACGTTAACAGCCTTCGGACGTTTGCCATGCATATCCAGGCTAATCCAGTCTTCGCCGACTTCGATGTCCGCTCCAGCGTCACGCAGTTTCTCCAGCACGGCg                                                                                                                    <  1:76166/140‑1 (MQ=255)             cggtcggGAATGTCGGATGCGGCGCGGTACGTACGTTAACAGCCTTCGGACGTTTGCCATGCATATCCAGGCTAATCCAGTCTTCGCCGACTTCGATGTCCGCTCCAGCGTCACGCAGTTTCTCCAGCACGGCGTCgaga                                                                                                              <  1:457649/140‑1 (MQ=255)               gtcggGAATGCCGGATGCGGCGCGGTACGTACGTTAACAGCCTTCGGACGTTTGCCATGCATATCCAGGCTAATCCAGTCTTCGCCGACTTCGATGTCCGCTCCAGCGTCACGCAGTTTCTCCAGCACGGCGTCGAGAGt                                                                                                            >  1:159526/1‑140 (MQ=255)                                                  aaCAGCCTTCGGACGTTTGCCATGCATATCCAGGCTAATCCAGTCTTCGCCGACTTCGATGTCCGCTCCAGCGTCACGCAGTTTCTCCAGCACGGCGTCGAGAGTATCTGGCTGCGCGTTACGGCAGATAATTTTGCcgc                                                                         >  2:555704/1‑140 (MQ=255)                                                       ccTTCGGACGTTTGCCATGCATATCCAGGCTAATCCAGTCTTCGCCGACTTCGATGTCCGCTCCAGCGTCACGCAGTTTCTCCAGCACGGCGTCGAGAGTATCTGGCTGCGCGTTACGGCAGATAATTTTGCCGCGAGaa                                                                    >  1:341197/1‑140 (MQ=255)                                                           cGGACGTTTGCCATGCATATCCAGGCTAATCCAGTCTTCGCCGACTTCGATGTCCGCTCCAGCGTCACGCAGTTTCTCCAGCACGGCGTCGAGAGTATCTGGCTGCGCGTTACGGCAGATAATTTTGCCGCGAGAAATcg                                                                >  1:74088/1‑140 (MQ=255)                                                                  ttGCCATGCATATCCAGGCTAATCCAGTCTTCGCCGACTTCGATGTCCGCTCCAGCGTCACGCAGTTTCTCCAGCACGGCGTCGAGAGTATCTGGCTGCGCCTTACGGCAGATAATTTTGCCGCGAGAAATcgccgccgc                                                         <  1:91896/140‑1 (MQ=255)                                                                     ccATGCATATCCAGGCTAATCCAGTCTTCGCCGACTTCGATGTCCGCTCCAGCGTCACGCAGTTTCTCCAGCACGGCGTCGAGAGTATCTGGCTGCGCGTTACGGCAGATAATTTTGCCGCGAGAAATCGCCGCCGccac                                                      <  1:28177/140‑1 (MQ=255)                                                                                cAGGCTAATCCAGTCTTCGCCGACTTCGATGTCCGCTCCAGCGTCACGCAGTTTCTCCAGCACGGCGTCGAGAGTATCTGGCTGCGCGTTACGGCAGATAATTTTGCCGCGAGAAATCGCCGCCGCCACCAGGAAAGTAc                                           <  1:392831/140‑1 (MQ=255)                                                                                  ggCTAATCCAGTCTTCGCCGACTTCGATGTCCGCTCCAGCGTCACGCAGTTTCTCCAGCACGGCGTCGAGAGTATCTGGCTGCGCGTTACGGCAGATAATTTTGCCGCGAGAAATCGCCGCCGCCACCAGGAAAGTACCg                                         >  1:106203/1‑140 (MQ=255)                                                                                   gCTAATCCAGTCTTCGCCGACTTCGATGTCCGCTCCAGCGTCACGCAGTTTCTCCAGCACGGCGTCGAGAGTATCTGGCTGCGCGTTACGGCAGATAATTTTGCCGCGAGAAATCGCCGCCGCCACCAGGAAAGTACCgg                                        >  2:282478/1‑140 (MQ=255)                                                                                   gCTAATCCAGTCTTCGCCGACTTCGATGTCCGCTCCAGCGTCACGCAGTTTCTCCAGCACGGCGTCGAGAGTATCTGGCTGCGCGTTACGGCAGATAATTTTGCCGCGAGAAATCGCCGCCGCCACCAGGAAAGTACCgg                                        >  2:338467/1‑140 (MQ=255)                                                                                    cTAATCCAGTCTTCGCCGACTTCGATGTCCGCTCCAGCGTCACGCAGTTTCTCCAGCACGGCGTCGAGAGTATCTGGCTGCGCGTTACGGCAGATAATTTTGCCGCGAGAAATCGCCGCCGCCACCAGGAAAGTACCGGt                                       <  2:545038/140‑1 (MQ=255)                                                                                      aaTCCAGTCTTCGCCGACTTCGATGTCCGCTCCAGCGTCACGCAGTTTCTCCAGCACGGCGTCGAGAGTATCTGGCTGCGCGTTACGGCAGATAATTTTGCCGCGAGAAATCGCCGCCGCCACCAGGAAAGTACCGGttt                                     <  1:589539/140‑1 (MQ=255)                                                                                       aTCCAGTCTTCGCCGACTTCGATGTCCGCTCCAGCGTCACGCAGTTTCTCCAGCACGGCGTCGAGAGTATCTGGCTGCGCGTTACGGCAGATAATTTTGCCGCGAGAAATCGCCGCCGCCACCAGGAAAGTACCGGTTTc                                    >  2:93991/1‑140 (MQ=255)                                                                                           aGTCTTCGCCGACTTCGATGTCCGCTCCAGCGTCACGCAGTTTCTCCAGCACGGCGTCGAGAGTATCTGGCTGCGCGTTACGGCAGATAATTTTGCCGCGAGAAATCGCCGCCGCCACCAGGAAAGTACCGGTTTCGATa                                <  2:472175/140‑1 (MQ=255)                                                                                                 cGCCGACTTCGATGTCCGCTCCAGCGTCACGCAGTTTCTCCAGCACGGCGTCGAGAGTATCTGGCTGCGCGTTACGGCAGATAATTTTGCCGCGAGAAATCGCCGCCGCCACCAGGAAAGTACCGGTTTCGATACGATcc                          >  2:324093/1‑140 (MQ=255)                                                                                                         tCGATGTCCGCTCCAGCGTCACGCAGTTTCTCCAGCACGGCGTCGAGAGTATCTGGCTGCGCGTTACGGCAGATAATTTTGCCGCGAGAAATCGCCGCCGCCACCAGGAAAGTACCGGTTTCGATACGATCCGGCAGAAc                  >  2:3081/1‑140 (MQ=255)                                                                                                          cGATGTCCGCTCCAGCGTCACGCAGTTTCTCCAGCACGGCGTCGAGAGTATCTGGCTGCGCGTTACGGCAGATAATTTTGCCGCGAGAAATCGCCGCCGCCACCAGGAAAGTACCGGTTTCGATACGATCCGGCAGAAcg                 <  2:341197/140‑1 (MQ=255)                                                                                                                ccGCTCCAGCGTCACGCAGTTTCTCCAGCACGGCGTCGAGAGTATCTGGCTGCGCGTTACGGCAGATAATTTTGCCGCGAGAAATCGCCGCCGCCACCAGGAAAGTACCGGTTTCGATACGATCCGGCAGAACGCGATAg           >  2:17984/1‑140 (MQ=255)                                                                                                                         cGTCACGCAGTTTCTCCAGCACGGCGTCGAGAGTATCTGGCTGCGCGTTACGGCAGATAATTTTGCCGCGAGAAATCGCCGCCGCCACCAGGAAAGTACCGGTTTCGATACGATCCGGCAGAACGCGATAGACAccgccg  >  1:60291/1‑140 (MQ=255)                                                                                                                                       |                                                                                                                              GGGCCTGCATATCGGTCGGGAATGCCGGATGCGGCGCGGTACGTACGTTAACAGCCTTCGGACGTTTGCCATGCATATCCAGGCTAATCCAGTCTTCGCCGACTTCGATGTCCGCTCCAGCGTCACGCAGTTTCGCCAGCACGGCGTCGAGAGTATCTGGCTGCGCGTTACGGCAGATAATTTTGCCGCGAGAAATCGCCGCCGCCACCAGGAAAGTACCGGTTTCGATACGATCCGGCAGAACGCGATAGACACCGCCG  >  NC_000913/3335570‑3335829

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑