Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	4,466,730	G→T	Q173K (CAG→AAG)	treR ←	DNA‑binding transcriptional repressor TreR

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	4,466,730	0	G	T	100.0%	70.5 / NA	21	Q173K (CAG→AAG)	treR	DNA‑binding transcriptional repressor TreR
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base T (10/11);  total (10/11)

TAGCAAGCCCTGGCAGGGCGGCAACGGGATGCAGTTTATGCGCTTTGCAGAACGCCAGGTAGGCTTCGTGACGTCGCTTACCGGTTGTCACGTCACTGTGCGGCACGCCGAGATAACTGATATTACGATGCCCCTGGTCATACAGCCGTTGCATCAGGATTTTGATTGCCCCTTCGTCGTCATAACAGACCGAAGCAAAGCCTTTTGCGTCACGCGCCAGCAGAACCAGCGATGACTGCCAGTGGGCTAACATTTCTTCTGTTATGC  >  NC_000913/4466595‑4466861                                                                                                                                        |                                                                                                                                    tAGCAAGCCCTGGCAGGGCGGCAACGGGATGCAGTTTATGCGCTTTGCAGAACGCCAGGTAGGCTTCGTGACGTCGCTTACCGGTTGTCACGTCACTGTGCGGCACGCCGAGATAACTGATATTACGATGCCCCTTGTCa                                                                                                                                 >  2:572808/1‑140 (MQ=255)                   cggcAACGGGATGCAGTTTATGCGCTTTGCAGAACGCCAGGTAGGCTTCGTGACGTCGCTTACCGGTTGTCACGTCACTGTGCGGCACGCCGAGATAACTGATATTACGATGCCCCTTGTCATACAGCCGTTGCATCAgg                                                                                                               >  2:533793/1‑140 (MQ=255)                           ggATGCAGTTTATGCGCTTTGCAGAACGCCAGGTAGGCTTCGTGACGTCGCTTACCGGTTGTCACGTCACTGTGCGGCACGCCGAGATAACTGATATTACGATGCCCCTTGTCATACAGCCGTTGCATCAGGATTTTGAt                                                                                                       <  1:128945/140‑1 (MQ=255)                                cAGTTTATGCGCTTTGCAGAACGCCAGGTAGGCTTCGTGACGTCGCTTACCGGTTGTCACGTCACTGTGCGGCACGCCGAGATAACTGATATTACGATGCCCCTTGTCATACAGCCGTTGCATCAGGATTTTGATTGccc                                                                                                  <  1:555919/140‑1 (MQ=255)                                    ttATGCGCTTTGCAGAACGCCAGGTAGGCTTCGTGACGTCGCTTACCGGTTGTCACGTCACTGTGCGGCACGCCGAGATAACTGATATTACGATGCCCCTTGTCATACAGCCGTTGCATCAGGATTTTGATTGCCCCTtc                                                                                              <  1:238894/140‑1 (MQ=255)                                    ttATGCGCTTTGCAGAACGCCAGGTAGGCTTCGTGACGTCGCTTACCGGTTGTCACGTCACTGTGCGGCACGCCGAGATAACTGATATTACGATGCCCCTTGTCATACAGCCGTTGCATCAGGATTTTGATTGCCCCTtc                                                                                              <  1:114669/140‑1 (MQ=255)                                              tGCAGAACGCCAGGTAGGCTTCGTGACGTCGCTTACCGGTTGTCACGTCACTGTGCGGCACGCCGAGATAACTGATATTACGATGCCCCTTGTCATACAGCCGTTGCATCAGGATTTTGATTGCCCCTTCGTCGTCATaa                                                                                    <  1:349339/140‑1 (MQ=255)                                                                  tCGTGACGTCGCTTACCGGTTGTCACGTCACTGTGCGGCACGCCGAGATAACTGATATTACGATGCCCCTTGTCATACAGCCGTTGCATCAGGATTTTGATTGCCCCTTCGTCGTCATAACAGACCGAAGCAAAGCCttt                                                                >  2:113553/1‑140 (MQ=255)                                                                  tCGTGACGTCGCTTACCGGTTGTCACGTCACTGTGCGGCACGCCGAGATAACTGATATTACGATGCCCCTTGTCATACAGCCGTTGCATCAGGATTTTGATTGCCCCTTCGTCGTCATAACAGACCGAAGCAAAGCCttt                                                                >  2:351727/1‑140 (MQ=255)                                                                         gTCGCTTACCGGTTGTCACGTCACTGTGCGGCACGCCGAGATAGCTGATATTACGATGCCCCTTGTCATACAGCCGTTGCATCAGGATTTTGATTGCCCCTTCGTCGTCATAACAGACCGAAGCAAAGCCTTTTGCGTCa                                                         <  2:350700/140‑1 (MQ=255)                                                                           cGCTTACCGGTTGTCACGTCACTGTGCGGCACGCCGAGATAACTGATATTACGATGCCCCTTGTCATACAGCCGTTGCATCAGGATTTTGATTGCCCCTTCGTCGTCATAACAGACCGAAGCAAAGCCTTTTGCGTCAcg                                                       >  2:433223/1‑140 (MQ=255)                                                                            gCTTACCGGTTGTCACGTCACTGTGCGGCACGCCGAGATAACTGATATTACGATGCCCCTTGTCATACAGCCGTTGCATCAGGATTTTGATTGCCCCTTCGTCGTCATAACAGACCGAAGCAAAGCCTTTTGCGTCAcgc                                                      <  1:203436/140‑1 (MQ=255)                                                                               tACCGGTTGTCACGTCACTGTGCGGCACGCCGAGATAACTGATATTACGATGCCCCTTGTCATACAGCCGTTGCATCAGGATTTTGATTGCCCCTTCGTCGTCATAACAGACCGAAGCAAAGCCTTTTGCGTCACGCGcc                                                   <  2:16733/140‑1 (MQ=255)                                                                                     ttGTCACGTCACTGTGCGGCACGCCGAGATAACTGATATTACGATGCCCCTTGTCATACAGCCGTTGCATCAGGATTTTGATTGCCCCTTCGTCGTCATAACAGACCGAAGCAAAGCCTTTTGCGTCACGCGCCAGCAGa                                             <  1:533793/140‑1 (MQ=255)                                                                                       gtcacgtcacTGTGCGGCACGCCGAGATAACTGATATTACGATGCCCCTTGTCATACAGCCGTTGCATCAGGATTTTGATTGCCCCTTCGTCGTCATAACAGACCGAAGCAAAGCCTTTTGCGTCACGCGCCAGCAGAAc                                           >  2:105370/1‑140 (MQ=255)                                                                                                               agaTAACTGATATTACGATGCCCCTTGTCATACAGCCGTTGCATCAGGATTTTGATTGCCCCTTCGTCGTCATAACAGACCGAAGCAAAGCCTTTTGCGTCACGCGCCAGCAGAACCAGCGATGACTGCCAGTGGGCTaa                   <  2:73926/140‑1 (MQ=255)                                                                                                                  tAACTGATATTACGATGCCCCTTGTCATACAGCCGTTGCATCAGGATTTTGATTGCCCCTTCGTCGTCATAACAGACCGAAGCAAAGCCTTTTGCGTCACGCGCCAGCAGAACCAGCGATGACTGCCAGTGGGCTAACAt                <  1:515242/140‑1 (MQ=255)                                                                                                                      tGATATTACGATGCCCCTTGTCATACAGCCGTTGCATCAGGATTTTGATTGCCCCTTCGTCGTCATAACAGACCGAAGCAAAGCCTTTTGCGTCACGCGCCAGCAGAACCAGCGATGACTGCCAGTGGGCTAACATttct            <  1:433223/140‑1 (MQ=255)                                                                                                                               gATGCCCCTTGTCATACAGCCGTTGCATCAGGATTTTGATTGCCCCTTCGTCGTCATAACAGACCGAAGCAAAGCCTTTTGCGTCACGCGCCAGCAGAACCAGCGATGACTGCCAGTGGGCTAACATTTCTTCTGTTATg   >  2:189402/1‑140 (MQ=255)                                                                                                                               gATGCCCCTTGTCATACAGCCGTTGCATCAGGATTTTGATTGCCCCTTCGTCGTCATAACAGACCGAAGCAAAGCCTTTTGCGTCACGCGCCAGCAGAACCAGCGATGACTGCCAGTGGGCTAACATTTCTTCTGTTATg   >  1:169363/1‑140 (MQ=255)                                                                                                                                aTGCCCCTTGTCATACAGCCGTTGCATCAGGATTTTGATTGCCCCTTCGTCGTCATAACAGACCGAAGCAAAGCCTTTTGCGTCACGCGCCAGCAGAACCAGCGATGACTGCCAGTGGGCTAACATTTCTTCTGTTATGc  >  2:19987/1‑140 (MQ=255)                                                                                                                                aTGCCCCTTGTCATACAGCCGTTGCATCAGGATTTTGATTGCCCCTTCGTCGTCATAACAGACCGAAGCAAAGCCTTTTGCGTCACGCGCCAGCAGAACCAGCGATGACTGCCAGTGGGCTAACATTTCTTCTGTTATGc  >  2:529027/1‑140 (MQ=255)                                                                                                                                        |                                                                                                                                    TAGCAAGCCCTGGCAGGGCGGCAACGGGATGCAGTTTATGCGCTTTGCAGAACGCCAGGTAGGCTTCGTGACGTCGCTTACCGGTTGTCACGTCACTGTGCGGCACGCCGAGATAACTGATATTACGATGCCCCTGGTCATACAGCCGTTGCATCAGGATTTTGATTGCCCCTTCGTCGTCATAACAGACCGAAGCAAAGCCTTTTGCGTCACGCGCCAGCAGAACCAGCGATGACTGCCAGTGGGCTAACATTTCTTCTGTTATGC  >  NC_000913/4466595‑4466861

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 31 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑