Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	1,556,991	G→T	A123S (GCA→TCA)	osmC →	osmotically inducible peroxiredoxin

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,556,991	0	G	T	100.0%	105.5 / NA	31	A123S (GCA→TCA)	osmC	osmotically inducible peroxiredoxin
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base T (13/18);  total (13/18)

ACCACCGCCGATGTGTCGCTGGATAAAGTGGATGCCGGTTTTGCGATTACGAAAATCGCACTGAAGAGTGAAGTTGCGGTGCCGGGTATTGATGCCTCTACCTTTGACGGCATAATCCAGAAAGCAAAAGCAGGATGCCCGGTCTCTCAGGTACTGAAAGCGGAAATTACGCTGGATTACCAGTTGAAATCGTAAAGCATTGCCGGATGACGCGTCAGGCGCGTGAATGCCTGATGCGTTGTTAGCATCTCTACAGCGCC  >  NC_000913/1556862‑1557121                                                                                                                                  |                                                                                                                                   accaccGCCGATGTGTCGCTGGATAAAGTGGATGCCGGTTTTGCGATTACGAAAATCGCACTGAAGAGTGAAGTTGCGGTGCCGGGTATTGATGCCTCTACCTTTGACGGCATAATCCAGAAAGCAAAATCAGGATGccc                                                                                                                          >  2:443511/1‑140 (MQ=255)  ccgccgccgATGTGTCGCTGGATAAAGTGGATGCCGGTTTTGCGATTACGAAAATCGCACTGAAGAGTGAAGTTGCGGTGCCGGGTATTGATGCCTCTACCTTTGACGGCATAATCCAGAAAGCAAAATCAGGATGCCCg                                                                                                                         <  2:61450/137‑1 (MQ=255)  ccaccGCCGATGTGTCGCTGGATAAAGTGGATGCCGGTTTTGCGATTACGAAAATCGCACTGAAGAGTGAAGTTGCGGTGCCGGGTATTGATGCCTCTACCTTTGACGGCATAATCCAGAAAGCAAAATCAGGATGCCCg                                                                                                                         <  2:547525/140‑1 (MQ=255)     ccgccgATGTGTCGCTGGATAAAGTGGATGCCGGTTTTGCGATTACGAAAATCGCACTGAAGAGTGAAGTTGCGGTGCCGGGTATTGATGCCTCTACCTTTGACGGCATAATCCAGAAAGCAAAATCAGGATGCCCGGtc                                                                                                                      >  2:518682/1‑140 (MQ=255)        ccgATGTGTCGCTGGATAAAGTGGATGCCGGTTTTGCGATTACGAAAATCGCACTGAAGAGTGAAGTTGCGGTGCCGGGTATTGATGCCTCTACCTTTGACGGCATAATCCAGAAAGCAAAATCAGGATGCCCGGtctct                                                                                                                   <  2:172032/140‑1 (MQ=255)          gATGTGTCGCTGGATAAAGTGGATGCCGGTTTTGCGATTACGAAAATCGCACTGAAGAGTGAAGTTGCGGTGCCGGGTATTGATGCCTCTACCTTTGACGGCATAATCCAGAAAGCAAAATCAGGATGCCCGGTCTCTCa                                                                                                                 >  1:111421/1‑140 (MQ=255)                tCGCTGGATAAAGTGGATGCCGGTTTTGCGATTACGAAAATCGCACTGAAGAGTGAAGTTGCGGTGCCGGGTATTGATGCCTCTACCTTTGACGGCATAATCCAGAAAGCAAAATCAGGATGCCCGGTCTCTCAGGTACt                                                                                                           <  1:424154/140‑1 (MQ=255)                               gATGCCGGTTTTGCGATTACGAAAATCGCACTGAAGAGTGAAGTTGCGGTGCCGGGTATTGATGCCTCTACCTTTGACGGCATAATCCAGAAAGCAAAATCAGGATGCCCGGTCTCTCAGGTACTGAAAGCGGAAATTAc                                                                                            <  1:134486/140‑1 (MQ=255)                                aTGCCGGTTTTGCGATTACGAAAATCGCACTGAAGAGTGAAGTTGCGGTGCCGGGTATTGATGCCTCTACCTTTGACGGCATAATCCAGAAAGCAAAATCAGGATGCCCGGTCTCTCAGGTACTGAAAGCGGAAATTACg                                                                                           >  2:533233/1‑140 (MQ=255)                                      gTTTTGCGATTACGAAAATCGCACTGAAGAGTGAAGTTGCGGTGCCGGGTATTGATGCCTCTACCTTTGACGGCATAATCCAGAAAGCAAAATCAGGATGCCCGGTCTCTCAGGTACTGAAAGCGGAAATTa                                                                                             <  2:220880/132‑1 (MQ=255)                                      gTTTTGCGATTACGAAAATCGCACTGAAGAGTGAAGTTGCGGTGCCGGGTATTGATGCCTCTACCTTTGACGGCATAATCCAGAAAGCAAAATCAGGATGCCCGGTCTCTCAGGTACTGAAAGCGGAAATTa                                                                                             >  1:220880/1‑132 (MQ=255)                                                tACGAAAATCGCACTGAAGAGTGAAGTTGCGGTGCCGGGTATTGATGCCTCTACCTTTGACGGCATAATCCAGAAAGCAAAATCAGGATGCCCGGTCTCTCAGGTACTGAAAGCGGAAATTACGCTGGATTACCAGTTGa                                                                           <  1:467716/140‑1 (MQ=255)                                                  cGAAAATCGCACTGAAGAGTGAAGTTGCGGTGCCGGGTATTGATGCCTCTACCTTTGACGGCATAATCCAGAAAGCAAAATCAGGATGCCCGGTCTCTCAGGTACTGAAAGCGGAAATTACGCTGGATTACCAGTTGaaa                                                                         <  2:471999/140‑1 (MQ=255)                                                          gCACTGAAGAGTGAAGTTGCGGTGCCGGGTATTGATGCCTCTACCTTTGACGGCATAATCCAGAAAGCAAAATCAGGATGCCCGGTCTCTCAGGTACTGAAAGCGGAAATTACGCTGGATTACCAGTTGAAATCGTAAAg                                                                 <  1:198698/140‑1 (MQ=255)                                                            aCTGAAGAGTGAAGTTGCGGTGCCGGGTATTGATGCCTCTACCTTTGACGGCATAATCCAGAAAGCAAAATCAGGATGCCCGGTCTCTCAGGTACTGAAAGCGGAAATTACGCTGGATTACCAGTTGAAATCGTAAAGCa                                                               <  2:158291/140‑1 (MQ=255)                                                                     tGAAGTTGCGGTGCCGGGTATTGATGCCTCTACCTTTGACGGCATAATCCAGAAAGCAAAATCAGGATGCCCGGTCTCTCAGGTACTGAAAGCGGAAATTACGCTGGATTACCAGTTGAAATCGTAAAGCATTGCCGGAt                                                      >  1:314372/1‑140 (MQ=255)                                                                     tGAAGTTGCGGCGCCGGGTATTGATGCCTCTACCTTTGACGGCATAATCCAGAAAGCAAAATCAGGATGCCCGGTCTCTCAGGTACTGAAAGCGGAAATTACGCTGGATTACCAGTTGAAATCGTAAAGCATTGCCGGAt                                                      >  1:113189/1‑140 (MQ=255)                                                                      gAAGTTGCGGTGCCGGGTATTGATGCCTCTACCTTTGACGGCATAATCCAGAAAGCAAAATCAGGATGCCCGGTCTCTCAGGTACTGAAAGCGGAAATTACGCTGGATTACCAGTTGAAATCGTAAAGCATTGCCGGATg                                                     >  2:174703/1‑140 (MQ=255)                                                                          ttGCGGTGCCGGGTATTGAAGCCTCTACCTTTGACGGCATAATCCAGAAAGCAAAATCAGGATGCCCGGTCTCTCAGGTACTGAAAGCGGAAATTACGCTGGATTACCAGTTGAAATCGTAAAGCATTGCCGGATGAcgc                                                 >  2:181508/1‑140 (MQ=255)                                                                               gTGCCGGGTATTGATGCCTCTACCTTTGACGGCATAATCCAGAAAGCAAAATCAGGATGCCCGGTCTCTCAGGTACTGAAAGCGGAAATTACGCTGGATTACCAGTTGAAATCGTAAAGCATTGCCGGATGACGCGTCAg                                            >  2:191654/1‑140 (MQ=255)                                                                                 gCCGGGTATTGATGCCTCTACCTTTGACGGCATAATCCAGAAAGCAAAATCAGGATGCCCGGTCTCTCAGGTACTGAAAGCGGAAATTACGCTGGATTACCAGTTGAAATCGTAAAGCATTGCCGGATGACGCGTCAGgc                                          <  2:499165/140‑1 (MQ=255)                                                                                 gCCGGGTATTGATGCCTCTACCTTTGACGGCATAATCCAGAAAGCAAAATCAGGATGCCCGGTCTCTCAGGTACTGAAAGCGGAAATTACGCTGGATTACCAGTTGAAATCGTAAAGCATTGCCGGATGACGCGTCAGgc                                          <  1:539645/140‑1 (MQ=255)                                                                                            aTGCCTCTACCTTTGACGGCATAATCCAGAAAGCAAAATCAGGATGCCCGGTCTCTCAGGTACTGAAAGCGGAAATTACGCTGGATTACCAGTTGAAATCGTAAAGCATTGCCGGATGACGCGTCAGGCGCGGGAATGcg                               >  1:564015/1‑139 (MQ=255)                                                                                                 tctACCTTTGACGGCATAATCCAGAAAGCAAAATCAGGATGCCCGGTCTCTCAGGTACTGAAAGCGGAAATTACGCTGGATTACCAGTTGAAATCGTAAAGCATTGCCGGATGACGCGTCAGGCGCGTGAATGCCTGATg                          >  2:258858/1‑140 (MQ=255)                                                                                                 tctACCTTTGACGGCATAATCCAGAAAGCAAAATCAGGATGCCCGGTCTCTCAGGTACTGAAAGCGGAAATTACGCTGGATTACCAGTTGAAATCGTAAAGCATTGCCGGATGACGCGTCAGGCGCGTGAATGCCTGATg                          <  2:389076/140‑1 (MQ=255)                                                                                                   tACCTTTGACGGCATAATCCAGAAAGCAAAATCAGGATGCCCGGTCTCTCAGGTACTGAAAGCGGAAATTACGCTGGATTACCAGTTGAAATCGTAAAGCATTGCCGGATGACGCGTCAGGCGCGTGAATGCCTGATGCg                        <  1:518682/140‑1 (MQ=255)                                                                                                   tACCTTTGACGGCATAATCCAGAAAGCAAAATCAGGATGCCCGGTCTCTCAGGTACTGAAAGCGGAAATTACGCTGGATTACCAGTTGAAATCGTAAAGCATTGCCGGATGACGCGTCAGGCGCGTGAATGCCTGATGCg                        >  1:490423/1‑140 (MQ=255)                                                                                                     ccTTTGACGGCATAATCCAGAAAGCAAAATCAGGATGCCCGGTCTCTCAGGTACTGAAAGCGGAAATTACGCTGGATTACCAGTTGAAATCGTAAAGCATTGCCGGATGACGCGTCAGGCGCGTGAATGCCTGATGCgtt                      <  2:470596/140‑1 (MQ=255)                                                                                                     ccTTTGACGGCATAATCCAGAAAGCAAAATCAGGATGCCCGGTCTCTCAGGTACTGAAAGCGGAAATTACGCTGGATTACCAGTTGAAATCGTAAAGCATTGCCGGATGACGCGTCAGGCGCGTGAATGCCTGATGCgtt                      <  2:47826/140‑1 (MQ=255)                                                                                                          gACGGCATAATCCAGAAAGCAAAATCAGGATGCCCGGTCTCTCAGGTACTGAAAGCGGAAATTACGCTGGATTACCAGTTGAAATCGTAAAGCATTGCCGGATGACGCGTCAGGCGCGTGAATGCCTGATGCGTTGTTAg                 <  2:314372/140‑1 (MQ=255)                                                                                                             ggCATAATCCAGAAAGCAAAATCAGGATGCCCGGTCTCTCAGGTACTGAAAGCGGAAATTACGCTGGATTACCAGTTGAAATCGTAAAGCATTGCCGGATGACGCGTCAGGCGCGTGAATGCCTGATGCGTTGTTAGCAt              <  2:154332/140‑1 (MQ=255)                                                                                                                         aaagcaaaatcaGGATGCCCGGTCTCTCAGGTACTGAAGGCGGAAATTACGCTGGATTACCAGTTGAAATCGTAAAGCATTGCCGGATGACGCGTCAGGCGCGTGAATGCCTGATGCGTTGTTAGCATCTCTACAGcgcc  >  1:168343/1‑140 (MQ=255)                                                                                                                                  |                                                                                                                                   ACCACCGCCGATGTGTCGCTGGATAAAGTGGATGCCGGTTTTGCGATTACGAAAATCGCACTGAAGAGTGAAGTTGCGGTGCCGGGTATTGATGCCTCTACCTTTGACGGCATAATCCAGAAAGCAAAAGCAGGATGCCCGGTCTCTCAGGTACTGAAAGCGGAAATTACGCTGGATTACCAGTTGAAATCGTAAAGCATTGCCGGATGACGCGTCAGGCGCGTGAATGCCTGATGCGTTGTTAGCATCTCTACAGCGCC  >  NC_000913/1556862‑1557121

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑