Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	1,841,612	G→T	P41P (CCG→CCT)	nudG →	5‑hydroxy‑CTP diphosphatase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,841,612	0	G	T	100.0%	115.7 / NA	34	P41P (CCG→CCT)	nudG	5‑hydroxy‑CTP diphosphatase
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base T (16/18);  total (16/18)

TGTGTAATTTATGAAAATGATTGAAGTTGTTGCCGCCATCATTGAACGTGATGGCAAAATTTTACTCGCGCAACGCCCCGCCCAGAGCGATCAGGCGGGATTATGGGAGTTTGCCGGTGGTAAAGTCGAGCCGGATGAAAGTCAGCGGCAGGCGCTGGTGCGTGAGTTACGCGAAGAACTGGGCATCGAAGCAACTGTGGGTGAATATGTTGCCAGCCATCAGCGAGAAGTTTCGGGGCGGATTATCCATCTTC  >  NC_000913/1841480‑1841733                                                                                                                                     |                                                                                                                          tgtgtAATTTATGAAAATGATTGAAGTTGTTGCCGCCATCATTGAACGTGATGGCAAAATTTTACTCGCGCAACGCCCCGCCCAGAGCGATCAGGCGGGATTATGGGAGTTTGCCGGTGGTAAAGTCGAGCCTGATGaaa                                                                                                                    <  1:448643/140‑1 (MQ=255)  gtgtAATTTATGAAAATGATTGAAGTTGTTGCCGCCATCATTGAACGTGATGGCAAAATTTTACTCGCGCAACGCCCCGCCCAGAGCGATCAGGCGGGATTATGGGAGTTTGCCGGTGGTAAAGTCGAGCCTGATGAAAg                                                                                                                   >  2:231872/1‑140 (MQ=255)  gtgtAATTTATGAAAATGATTGAAGTTGTTGCCGCCATCATTGAACGTGATGGCAAAATTTTACTCGCGCAACGCCCCGCCCAGAGCGATCAGGCGGGATTATGGGAGTTTGCCGGTGGTAAAGTCGAGCCTGATGAAAg                                                                                                                   >  1:506052/1‑140 (MQ=255)   tgtAATTTATGAAAATGATTGAAGTTGTTGCCGCCATCATTGAACGTGATGGCAAAATTTTACTCGCGCAACGCCCCGCCCAGAGCGATCAGGCGGGATTATGGGAGTTTGCCGGTGGTAAAGTCGAGCCTGATGAAAGt                                                                                                                  >  2:284292/1‑140 (MQ=255)   tgtAATTTATGAAAATGATTGAAGTTGTTGCCGCCATCATTGAACGTGATGGCAAAA‑TTTACTCGCGCAACGCCCCGCCCAGAGCGATCAGGCGGGATTATGGGAGTTTGCCGGTGGTAAAGTCGAGCCTGATGAAAGTc                                                                                                                 >  2:35411/1‑140 (MQ=255)           aTGAAAATGATTGAAGTTGTTGCCGCCATCATTGAACGTGATGGCAAAATTTTACTCGCGCAACGCCCCGCCCAGAGCGATCAGGCGGGATTATGGGAGTTTGCCGGTGGTAAAGTCGAGCCTGATGAAAGTCAGCGGCa                                                                                                          >  1:109260/1‑140 (MQ=255)           aTGAAAATGATTGAAGTTGTTGCCGCCATCATTGAACGTGATGGCAAAATTTTACTCGCGCAACGCCCCGCCCAGAGCGATCAGGCGGGATTATGGGAGTTTGCCGGTGGTAAAGTCGAGCCTGATGAAAGTCAGCGGCa                                                                                                          >  2:556450/1‑140 (MQ=255)            tGAAAATGATTGAAGTTGTTGCCGCCATCATTGAACGTGATGGCAAAATTTTACTCGCGCAACGCCCCGCCCAGAGCGATCAGGCGGGATTATGGGAGTTTGCCGGTGGTAAAGTCGAGCCTGATGAAAGTCAGCGGCAg                                                                                                         >  2:44050/1‑140 (MQ=255)                aaTGATTGAAGTTGTTGCCGCCATCATTGAACGTGATGGCAAAATTTTACTCGCGCAACGCCCCGCCCAGAGCGATCAGGCGGGATTATGGGAGTTTGCCGGTGGTAAAGTCGAGCCTGATGAAAGTCAGCGGCAGgcgc                                                                                                     >  1:116448/1‑140 (MQ=255)                      tGAAGTTGTTGCCGCCATCATTGAACGTGATGGCAAAATTTTACTCGCGCAACGCCCCGCCCAGAGCGATCAGGCGGGATTATGGGAGTTTGCCGGTGGTAAAGTCGAGCCTGATGAAAGTCAGCGGCAGGCGCTGGTGc                                                                                               <  1:556450/140‑1 (MQ=255)                           ttgttgCCGCCATCATTGAACGTGATGGCAAAATTTTACTCGCGCAACGCCCCGCCCAGAGCGATCAGGCGGGATTATGGGAGTTTGCCGGTGGTAAAGTCGAGCCTGATGAAAGTCAGCGGCAGGCGCTGGTGCGTGAg                                                                                          <  2:282147/140‑1 (MQ=255)                             gttgCCGCCATCATTGAACGTGATGGCAAAATTTTACTCGCGCAACGCCCCGCCCAGAGCGATCAGGCGGGATTATGGGAGTTTGCCGGTGGTAAAGTCGAGCCTGATGAAAGTCAGCGGCAGGCGCTGGTGCGTGAGtt                                                                                        >  1:244017/1‑140 (MQ=255)                                  cgccATCATTGAACGTGATGGCAAAATTTTACTCGCGCAACGCCCCGCCCAGAGCGATCAGGCGGGATTATGGGAGTTTGCCGGTGGTAAAGTCGAGCCTGATGAAAGTCAGCGGCAGGCGCTGGTGCGTGAGTTAcgcg                                                                                   >  1:135369/1‑140 (MQ=255)                                  cgccATCATTGAACGTGATGGCAAAATTTTACTCGCGCAACGCCCCGCCCAGAGCGATCAGGCGGGATTATGGGAGTTTGCCGGTGGTAAAGTCGAGCCTGATGAAAGTCAGCGGCAGGCGCTGGTGCGTGAGTTAcgcg                                                                                   <  2:343704/140‑1 (MQ=255)                                       tcatTGAACGTGATGGCAAAATTTTACTCGCGCAACGCCCCGCCCAGAGCGATCAGGCGGGATTATGGGAGTTTGCCGGTGGTAAAGTCGAGCCTGATGAAAGTCAGCGGCAGGCGCTGGTGCGTGAGTTACGCgaagaa                                                                              <  2:406091/140‑1 (MQ=255)                                                 tGATGGCAAAATTTTACTCGCGCAACGCCCCGCCCAGAGCGATCAGGCGGGATTATGGGAGTTTGCCGGTGGTAAAGTCGAGCCTGATGAAAGTCAGCGGCAGGCGCTGGTGCGTGAGTTACGCGAAGAACTGGGCATCg                                                                    <  1:573361/140‑1 (MQ=255)                                                  gATGGCAAAATTTTACTCGCGCAACGCCCCGCCCAGAGCGATCAGGCGGGATTATGGGAGTTTGCCGGTGGTAAAGTCGAGCCTGATGAAAGTCAGCGGCAGGCGCTGGTGCGTGAGTTACGCGAAGAACTGGGCATCGa                                                                   >  2:4514/1‑140 (MQ=255)                                                   aTGGCAAAATTTTACTCGCGCAACGCCCCGCCCAGAGCGATCAGGCGGGATTATGGGAGTTTGCCGGTGGTAAAGTCGAGCCTGATGAAAGTCAGCGGCAGGCGCTGGTGCGTGAGTTACGCGAAGAACTGGGCATCGaa                                                                  <  2:196424/140‑1 (MQ=255)                                                       cAAAATTTTACTCGCGCAACGCCCCGCCCAGAGCGATCAGGCGGGATTATGGGAGTTTGCCGGTGGTAAAGTCGAGCCTGATGAAAGTCAGCGGCAGGCGCTGGTGCGTGAGTTACGCGAAGAACTGGGCATCGAAGCaa                                                              <  1:44050/140‑1 (MQ=255)                                                          aaTTTTACTCGCGCAACGCCCCGCCCAGAGCGATCAGGCGGGATTATGGGAGTTTGCCGGTGGTAAAGTCGAGCCTGATGAAAGTCAGCGGCAGGCGCTGGTGCGTGAGTTACGCGAAGAACTGGGCATCGAAGCAACtg                                                           <  1:456745/140‑1 (MQ=255)                                                           aTTTTACTCGCGCAACGCCCCGCCCAGAGCGATCAGGCGGGATTATGGGAGTTTGCCGGTGGTAAAGTCGAGCCTGATGAAAGTCAGCGGCAGGCGCTGGTGCGTGAGTTACGCGAAGAACTGGGCATCGAAGCAACtgt                                                          <  2:116448/140‑1 (MQ=255)                                                             tttACTCGCGCAACGCCCCGCCCAGAGCGATCAGGCGGGATTATGGGAGTTTGCCGGTGGTAAAGTCGAGCCTGATGAAAGTCAGCGGCAGGCGCTGGTGCGTGAGTTACGCGAAGAACTGGGCATCGAAGCAACTGTgg                                                        <  2:276375/140‑1 (MQ=255)                                                               tACTCGCGCAACGCCCCGCCCAGAGCGATCAGGCGGGATTATGGGAGTTTGCCGGTGGTAAAGTCGAGCCTGATGAAAGTCAGCGGCAGGCGCTGGTGCGTGAGTTACGCGAAGAACTGGGCATCGAAGCAACTGTGGGt                                                      <  1:413223/140‑1 (MQ=255)                                                               tACTCGCGCAACGCCCCGCCCAGAGCGATCAGGCGGGATTATGGGAGTTTGCCGGTGGTAAAGTCGAGCCTGATGAAAGTCAGCGGCAGGCGCTGGTGCGTGAGTTACGCGAAGAACTGGGCATCGAAGCAACTGTGGGt                                                      <  1:4514/140‑1 (MQ=255)                                                                      gcAACGCCCCGCCCAGAGCGATCAGGCGGGATTATGGGAGTTTGCCGGTGGTAAAGTCGAGCCTGATGAAAGTCAGCGGCAGGCGCTGGTGCGTGAGTTACGCGAAGAACTGGGCATCGAAGCAACTGTGGGTGAATATg                                               >  1:503564/1‑140 (MQ=255)                                                                                       gCGATCAGGCGGGATTATGGGAGTTTGCCGGTGGTAAAGTCGAGCCTGATGAAAGTCAGCGGCAGGCGCTGGTGCGTGAGTTACGCGAAGAACTGGGCATCGAAGCAACTGTGGGTGAATATGTTGCCAGCCATCAGCga                              >  1:427571/1‑140 (MQ=255)                                                                                           tCAGGCGGGATTATGGGAGTTTGCCGGTGGTAAAGTCGAGCCTGATGAAAGTCAGCGGCAGGCGCTGGTGCGTGAGTTACGCGAAGAACTGGGCATCGAAGCAACTGTGGGGGAATATGTTGCCAGCCATCAGCGAGAAg                          >  2:215891/1‑140 (MQ=255)                                                                                                   gATTATGGGAGTTTGCCGGTGGTAAAGTCGAGCCTGATGAAAGTCAGCGGCAGGCGCTGGTGCGTGAGTTACGCGAAGAACTGGGCATCGAAGCAACTGTGGGTGAATATGTTGCCAGCCATCAGCGAGAAGTTTCgggg                  <  1:284292/140‑1 (MQ=255)                                                                                                    aTTATGGGAGTTTGCCGGTGGTAAAGTCGAGCCTGATGAAAGTCAGCGGCAGGCGCTGGTGCGTGAGTTACGCGAAGAACTGGGCATCGAAGCAACTGTGGGTGAATATGTTGCCAGCCATCAGCGAGAAGTTTCGGGGc                 >  1:496257/1‑140 (MQ=255)                                                                                                     ttATGGGAGTTTGCCGGTGGTAAAGTCGAGCCTGATGAAAGTCAGCGGCAGGCGCTGGTGCGTGAGTTACGCGAAGAACTGGGCATCGAAGCAACTGTGGGTGAATATGTTGCCAGCCATCAGCGAGAAGTTTCGGGGCg                <  1:271174/140‑1 (MQ=255)                                                                                                             gTTTGCCGGTGGTAAAGTCGAGCCTGATGAAAGTCAGCGGCAGGCGCTGGTGCGTGAGTTACGCGAAGAACTGGGCATCGAAGCAACTGTGGGTGAATATGTTGCCAGCCATCAGCGAGAAGTTTCGGGGCGGATTATcc        <  2:390119/140‑1 (MQ=255)                                                                                                                tGCCGGTGGTAAAGTCGAGCCTGATGAAAGTCAGCGGCAGGCGCTGGTGCGTGAGTTACGCGAAGAACCGGGCATCGAAGCAACTGTGGGTGAATATGTTGCCAGCCATCAGCGAGAAGTTTCGGGGCGGATTATCCATc     >  1:527464/1‑140 (MQ=255)                                                                                                                  ccGGTGGTAAAGTCGAGCCTGATGAAAGTCAGCGGCAGGCGCTGGTGCGTGAGTTACGCGAAGAACTGGGCATCGAAGCAACTGTGGGTGAATATGTTGCCAGCCATCAGCGAGAAGTTTCGGGGCGGATTATCCATCtt   <  1:462210/140‑1 (MQ=255)                                                                                                                   cGGTGGTAAAGTCGAGCCTGATGAAAGTCAGCGGCAGGCGCTGGTGCGTGAGTTACGCGAAGAACTGGGCATCGAAGCAACTGTGGGTGAATATGTTGCCAGCCATCAGCGAGAAGTTTCGGGGCGGATTATCCATCTTc  <  1:446914/140‑1 (MQ=255)                                                                                                                                     |                                                                                                                          TGTGTAATTTATGAAAATGATTGAAGTTGTTGCCGCCATCATTGAACGTGATGGCAAAATTTTACTCGCGCAACGCCCCGCCCAGAGCGATCAGGCGGGATTATGGGAGTTTGCCGGTGGTAAAGTCGAGCCGGATGAAAGTCAGCGGCAGGCGCTGGTGCGTGAGTTACGCGAAGAACTGGGCATCGAAGCAACTGTGGGTGAATATGTTGCCAGCCATCAGCGAGAAGTTTCGGGGCGGATTATCCATCTTC  >  NC_000913/1841480‑1841733

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 31 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑