Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_000913753,1250TA23.5% 72.9 / 10.8 34intergenic (‑330/+60)ybgD/gltAputative fimbrial protein YbgD/citrate synthase
Reads supporting (aligned to +/- strand):  ref base T (21/5);  new base A (0/8);  total (21/13)
Fisher's exact test for biased strand distribution p-value = 7.09e-05
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00
Rejected as consensus: Frequency below/above cutoff threshold.

ATGAAGTTTTTAATTGAAAGTATTGGGTTGCTGATAATTTGAGCTGTTCTATTCTTTTTAAATATCTATATAGGTCTGTTAATGGATTTTATTTTTACAATTTTTTGTGTTTAGGCATATAAAAATCAACCCGCCATATGAACGGCGGGTTAAAATATTTACAACTTAGCAATCAACCATTAACGCTTGATATCGCTTTTAAAGTCGCGTTTTTCATATCCTGTATACAGCTGACGCGGACGGGCAATCTTCATACCGT  >  NC_000913/753006‑753264
                                                                                                                       |                                                                                                                                           
aTGAAGTTTTTAATTGAAAGTATTGGGTTGCTGATAATTTGAGCTGTTCTATTCTTTTTAAATATCTATATAGGTCTGTTAATGGATTTTATTTTTACAATTTTTTGTGTTTAGGCATATAAAAATCAACCCGCCATATg                                                                                                                         >  1:663210/1‑140 (MQ=255)
     ttttttAATTGAAAGTATTGGTTGGTTTATAATTTGTGTTTTTTTATTATTTTAAATAATCTATTGAGTTCTTTAAATGGATTTTTTTTTTAAAATTTTTTGTTTTTAGTAAAAAAAAAATCATCCCGCCATATGAAcgg                                                                                                                    <  2:1193192/139‑1 (MQ=255)
         ttAATTGAAAGTATTGGGTTGCTGATAATTTGAGCTGTTCTATTCTTTTTAAATATCTATATAGGTCTGTTAATGGATTTTATTTTTACAATTTTTTGTGTTTAGGCATATAAAAATCAACCCGCCATATGAACGGCggg                                                                                                                >  1:386735/1‑140 (MQ=255)
                           ttGCTGATAATTTGAGCTGTTCTATTCTTTTTAAATATCTATATAGGTCTGTTAATGGATTTTATTTTTACAATTTTTTGTGTTTAGGCATATAAAAATCAACCCGCCATATGAACGGCGGGTTAAAATATTTACAACtt                                                                                              >  1:925867/1‑140 (MQ=255)
                              cTGATAATTTGAGCTGTTCTATTCTTTTTAAATATCTATATAGGTCTGTTAATGGATTTTATTTTTACAATTTTTTGTGTTTAGGCATATAAAAATCAACCCGCCATATGAACGGCGGGTTAAAATATTTACAACTTAGc                                                                                           >  1:646643/1‑140 (MQ=255)
                                        gAGCTTTTTTTTTTTTTTTAAATATTTATATAGGTTTTTTAATGGTTTTTTTTTTTACAATTTTTTGTTTTTAGGAAAAAAAAAATCACCCCGCCATATGAACGGCGGGTTAAAATATTTACAACTTAGCAATCAACCAt                                                                                 <  2:1008212/140‑1 (MQ=255)
                                                   ttCTTTTTAAATATCTATATAGGTCTGTTAATGGATTTTATTTTTACAATTTTTTGTGTTTAGGCATATAAAAATCAACCCGCCATATGAACGGCGGGTTAAAATATTTACAACTTAGCAATCAACCATTAAAGCTTGat                                                                      >  1:109838/1‑140 (MQ=255)
                                                      ttttaAATTATTTATATAGGTTTTTTAATGATTTTTATTTTTAAAATTTTTTGTGTTTAGGAATAAAAAAATCACCCCGCCATATGAACGGCGGGTTAAAATATTTACAACTTAGCAATCAACCATTAACGCTTGATATc                                                                   <  2:210775/140‑1 (MQ=255)
                                                        tttAAATATCTATATAGGTCTGTTAATGGATTTTATTTTTACAATTTTTTGTGTTTAGGCATATAAAAATCAACCCGCCATATGAACGGCGGGTTAAAATATTTACAACTTAGCAATCAACCATTAACGCTTGATATCGc                                                                 >  1:738638/1‑140 (MQ=255)
                                                        tttAAATATCTATATAGGTCTGTTAATGGATTTTATTTTTACAATTTTTTGTGTTTAGGCATATAAAAATCAACCCGCCATATGAACGGCGGGTTAAAATATTTACAACTTAGCAATCAACCATTAACGCTTGATATCGc                                                                 >  1:854305/1‑140 (MQ=255)
                                                        tttAAATATCTATATAGGTCTGTTAATGGATTTTATTTTTACAATTTTTTGTGTTTAGGCATATAAAAATCAACCCGCCATATGAACGGCGGGTTAAAATATTTACAACTTAGCAATCAACCATTAACGCTTGATATCGc                                                                 >  1:210775/1‑140 (MQ=255)
                                                        tttAAATATCTATATAGGTCTGTTAATGGATTTTATTTTTACAATTTTTTGTGTTTAGGCATATAAAAATCAACCCGCCATATGAACGGCGGGTTAAAATATTTACAACTTAGCAATCAACCATTAACGCTTGATATCGc                                                                 >  1:1083413/1‑140 (MQ=255)
                                                                      taGGTTTTTAAATGGTTTTTATTTTTAAAATTTTTTGTTTTAAGAAAAAAAAAAATCAACCCGCCATATGAACGGCGGGTTAAAATATTTACAACTTAGCAATCAACCATTAACGCTTGATATCGCTTTTAAAGTCGCGt                                                   <  2:1083413/140‑1 (MQ=255)
                                                                      taGGTCTTTTAATGGTTTTTATTTTTACAATTTTTTGTTTTTAGGAAAAAAAAAACCAACCCGCCATATGAACGGCGGGTTAAAATATTTACAACTTAGCAATCAACCATTAACGCTTGATATCGCTTTTAAAGTCGCGt                                                   <  2:935026/140‑1 (MQ=255)
                                                                         gTCTGTTAATGGATTTTATTTTTACAATTTTTTGTGTTTAGGCATATAAAAATCAACCCGCCATATGAACGGCGGGTTAAAATATTTACAACTTAGCAATCAACCATTAACGCTTGATATCGCTTTTAAAGTCGCGtttt                                                >  1:903790/1‑140 (MQ=255)
                                                                         gTCTGTTAATGGATTTTATTTTTACAATTTTTTGTGTTTAGGCATATAAAAATCAACCCGCCATATGAACGGCGGGTTAAAATATTTACAACTTAGCAATCAACCATTAACGCTTGATATCGCTTTTAAAGTCGCGtttt                                                >  1:1049236/1‑140 (MQ=255)
                                                                         gTCTGTTAATGGATTTTATTTTTACAATTTTTTGTGTTTAGGCATATAAAAATCAACCCGCCATATGAACGGCGGGTTAAAATATTTACAACTTAGCAATCAACCATTAACGCTTGATATCGCTTTTAAAATCGCGtttt                                                >  2:568963/1‑140 (MQ=255)
                                                                           ttGTTAATGGATTTTATTTTTACAATTTTTTGTTTTTAGGCATAAAAAAATCAACCCGCCATATGAACGGCGGGTTAAAATATTTACAACTTAGCAATCAACCATTAACGCTTGATATCGCTTTTAAAGTCGCGTTTTTc                                              <  1:1055777/139‑1 (MQ=255)
                                                                            tGTTAATGGATTTTATTTTTACAATTTTTTGTGTTTAGGCATATAAAAATCAACCCGCCATATGAACGGCGGGTTAAAATATTTACAACTTAGCAATCAACCATTAACGCTTGATATCGCTTTTAAAGTCGCGTTTTTCa                                             >  1:182933/1‑140 (MQ=255)
                                                                            tGTTAATGGATTTTATTTTTACAATTTTTTGTGTTTAGGCATATAAAAATCAACCCGCCATATGAACGGCGGGGTAAAATATTTACAACTTAGCAATCAACCATTAAAGCCTGATATCCCTTTTTAAGTAGCGTTTTTCa                                             >  2:448303/1‑140 (MQ=255)
                                                                             tttAATGTTTTTTTTTTTTACAATTTTTTGTGTTTAGGCATAAAAAAAACAACCCGCCATATGAACGGCGGGTTAAAATATTTACAACTTAGCAATCAACCATTAACGCTTGATATCGCTTTTAAAGTCGCGTCTTTCat                                            <  2:854305/139‑1 (MQ=255)
                                                                             tttAATGTTTTTTTTTTTTAAATTTTTTTTTTTTTAGGAATAAAAAAATCACCCCGCCATATGAACGGCGGGTTAAAATATTTACAACTTAGCAATCAACCATTAACGCTTGATATCGCTTTTAAAGTCGCGTTTTTCat                                            <  2:925867/139‑1 (MQ=255)
                                                                             tttAATGGTTTTTTTTTTAAAATTTTTTTTTTTTTAGACATAAAAAAACCAACCCGCCATATGAACGGCGGGTTAAAATATTTACAACTTAGCAATCAACCATTAACGCTTGATATCGCTTTTAAAGTCGCGTTTTTCat                                            <  2:182933/139‑1 (MQ=255)
                                                                             tttAATGGTTTTTATTTTTACATTTTTTTTTGTTTAGGAATATAAAAATCAACCCGCCATATGAACGGCGGGTTAAAATATTTACAACTTAGCAATCAACCATTAACGCTTGATATCGCTTTTAAAGTCGCGTTTTTCat                                            <  2:580875/139‑1 (MQ=255)
                                                                             tttAATGATTTTTATTTTTACAATTTTTTGTGTTTAGGAATAAAAAAACCAACCCGCCATATGAACGGCGGGTTAAAATATTTACAACTTAGCAATCAACCATTAACGCTTGATATCGCTTTTAAAGTCGCGTTTTTCat                                            <  2:367809/139‑1 (MQ=255)
                                                                             gTTAATGGTTTTTTTTTTTACATTTTTTTGTTTTTAGGCAAAAAAAAACCAACCCGCCATATGAACGGCGGGTTAAAATATTTACAACTTAGCAATCAACCATTAACGCTTGATATCGCTTTTAAAGTCGCGTTTTTCat                                            <  2:646643/140‑1 (MQ=255)
                                                                             gTTAAGGTATTTTATTTTTACATTTTTTTTTGTTAAGCAATATAAAAAACACCCCGCCATATGAACGGCGGGTTAAAATATTTACAACTTAGCAATCAACCATTAACGCTTGATATCGCTTTTAAAGTCGCGTTTTTCat                                            <  2:778305/140‑1 (MQ=255)
                                                                              ttAATGGATTTTATTTTTACAATTTTTTGTGTTTAGGCATATAAAAATCAACCCGCCATATGAACGGCGGGTTAAAATATTTACAACTTAGCAATCAACCATTAACGCTTGATATCGCTTTTAAAGTCGCGTTTTTCata                                           >  2:112374/1‑140 (MQ=255)
                                                                              ttAATGGATTTTATTTTTACAATTTTTTGTGTTTAGGCATATAAAAATCAACCCGCCATATGAACGGCGGGTTAAAATATTTACAACTTAGCAATCAACCATTAACGCTTGATATCGCTTTTAAAGTCGCGTTTTTCata                                           >  2:189907/1‑140 (MQ=255)
                                                                              ttAATGGATTTTATTTTTACAATTTTTTGTGTTTAGGCATATAAAAATCAACCCGCCATATGAACGGCGGGTTAAAATATTTACAACTTAGCAATCAACCATTAACGCTTGATATCGCTTTTAAAGTCGCGTTTTTCata                                           >  2:854992/1‑140 (MQ=255)
                                                                              ttAATGGATTTTATTTTTACAATTTTTTGTGTTTAGGCATATAAAAATCAACCCGCCATATGAACGGCGGGTTAAAATATTTACAACTTAGCAATCAACCATTAACGCTTGATATCGCTTTTAAAGTCGCGTTTTTCata                                           >  1:367809/1‑140 (MQ=255)
                                                                               tAATGTATTTTATTTTTAAATTTTTTTGTGTTTAGGCATATAAAAATCAGCCCGCCATATGAACGGCGGGTTAAAATATTTACAACTTAGCAATCAACCATTAACGCTT‑ATATCGCTTTTAAAGTCGCGTTTTTCATATc                                         <  1:142685/140‑1 (MQ=255)
                                                                                aatgttttttttttttAAATTTTTTTGTTTTTAGGCATAAAAAAATCACCCCGCCATATGAACGGCGGGTTAAAATATTTACAACTTAGCAATCAACCATTAACGCTTGATATCGCTTTTAAAGTCGCGTTTTTCATATc                                         <  2:557813/134‑1 (MQ=255)
                                                                                aaTGGTTTTTATTTTTAAATTTTTTTTTTTTAAGGCAAAAAAAAACCACCCCGCCATATGAACGGCGGGTTAAAATATTTACAACTTAGCAATCAACCATTAACGCTTGGTATCGCTTTTAAAGTCGCGTTTTTCATATc                                         <  2:899183/140‑1 (MQ=255)
                                                                                aaTGGATTTTTTTTTTACATTTTTTTGTGTTTAGGCATATAAAAATCAACCCGCCATATGAACGGCGGGTTAAAATATTTACAACTTAGCAATCAACCATTAACGCTTGATATCGCTTTTAAAGTCGCGTTTTTCATATc                                         <  1:1105141/140‑1 (MQ=255)
                                                                                        ttattttTACAATTTTTTGTGTTTAGGCATATAAAAATCAACCCGCCATATGAACGGCGGGTTAAAATATTTACAACTTAGCAATCAACCATTAACGCTTGATATCGCTTTTAAAGTCGCGTTTTTCATATCCTGTATAc                                 >  1:557813/1‑140 (MQ=255)
                                                                                               tACAATTTTTTGTGTTTAGGCATATAAAAATCAACCCGCCATATGAACGGCGGGTTAAAATATTTTCAACTTAGCAATCAACCATTAAAGCTTGATATCGCTTTTAAAGTCGCGTTTTTCATATCCTGTATACAGCTGAc                          >  2:18611/1‑140 (MQ=255)
                                                                                                         tgttTTTAGGCATAAAAAAATCACCCCGCCATATGAACGGCGGGTTAAAATATTTACAACTTAGCAATCAACCATTAACGCTTGATATCGCTTTTAAAGTCGCGTTTTTCATATCCTGTATACAGCTGACGCGGACGGGc                <  1:448303/140‑1 (MQ=255)
                                                                                                         tgttTTTAGGAATAAAAAAATCACCCCGCCATATGAACGGCGGGTTAAAATATTTACAACTTAGCAATCAACCATTAACGCTTGATATCGCTTTTAAAGTCGCGTTTTTCATATCCTGTATACAGCTGACGCGGACGGGc                <  2:956769/140‑1 (MQ=255)
                                                                                                         tgtgTTTAGGCATATAAAAATCAACCCGCCATATGAACGGCGGGTTAAAATATTTACAACTTAGCAATCAACCATTAACGCTTGATATCGCTTTTAAAGTCGCGTTTTTCATATCCTGTATACAGCTGACGCGGACGGGc                <  1:854992/140‑1 (MQ=255)
                                                                                                               tAGGCATATAAAAATCAACCCGCCATATGAACGGCGGGTTAAAATATTTACAACTTAGCAATCAACCATTAACGCTTGATATCGCTTTTAAAGTCGCGTTTTTCATATCCTGTATACAGCTGACGCGGACGGGCAATCtt          <  1:189907/140‑1 (MQ=255)
                                                                                                                  gCATATAAAAATCAACCCGCCATATGAACGGCGGGTTAAAATATTTACAACTTAGCAATCAACCATTAACGCTTGATATCGCTTTTAAAGTCGCGTTTTTCATATCCTGTATACAGCTGACGCGGACGGGCAATCTTCAt       >  2:470328/1‑140 (MQ=255)
                                                                                                                     tataAAAATCAACCCGCCATATGAACGGCGGGTTAAAATATTTACAACTTAGCCATCAACCATTAACGCTTGATATCGCTTTTAAAGTCGCGTTTTTCATATCCTGTATACAGCTGACGCGGACGGGCAATCTTCATAcc    >  1:995247/1‑140 (MQ=255)
                                                                                                                       taAAAATCAACCCGCCATATGAACGGCGGGTTAAAATATTTACAACTTAGCAATCAACCATTAACGCTTGATATCGCTTTTAAAGTCGCGTTTTTCATATCCTGTATACAGCTGACGCGGACGGGCAATCTTCATACCGt  >  1:602779/1‑140 (MQ=255)
                                                                                                                       taAAAATCAACCCGCCATATGAACGGCGGGTTAAAATATTTACAACTTAGCAATCAACCATTAACGCTTGATATCGCTTTTAAAGGCGCGTTTTTCATATCCTGTATACAGCTGACGCGGACGGGCAATCTTCATACCGt  >  1:486887/1‑140 (MQ=255)
                                                                                                                       |                                                                                                                                           
ATGAAGTTTTTAATTGAAAGTATTGGGTTGCTGATAATTTGAGCTGTTCTATTCTTTTTAAATATCTATATAGGTCTGTTAATGGATTTTATTTTTACAATTTTTTGTGTTTAGGCATATAAAAATCAACCCGCCATATGAACGGCGGGTTAAAATATTTACAACTTAGCAATCAACCATTAACGCTTGATATCGCTTTTAAAGTCGCGTTTTTCATATCCTGTATACAGCTGACGCGGACGGGCAATCTTCATACCGT  >  NC_000913/753006‑753264

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 7 ≤ ATCG/ATCG < 19 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑