Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	79,171	C→A	D95Y (GAC→TAC)	leuD ←	3‑isopropylmalate dehydratase subunit LeuD

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	79,171	0	C	A	100.0%	240.0 / NA	68	D95Y (GAC→TAC)	leuD	3‑isopropylmalate dehydratase subunit LeuD
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base A (32/36);  total (32/36)

GATTAGCTTTCACCAGCGCAAACAGTTCGTCCACTTCTGCATCGCTTAATTTCACCGGCAGCAGCTGGTTGTTAAAGCTATTGCCGTAGAAGATGTCAGCAAAACTCGGCGCAATCACCACTTTAAAACCGTAGTCGGTCAATGCCCAGGGCGCGTGCTCACGCGAAGAGCCACAGCCGAAGTTTTCTCGTGCCAGCAAAATGGAAGCGCCCTGATACTGCGGGAAGTTCAGCACGAAGTCCGGGTTTGGCTGTTGGCCTTTTTCATCCAGA  >  NC_000913/79036‑79307                                                                                                                                        |                                                                                                                                         gATTAGCTTTCACCAGCGCAAACAGTTCGTCCACTTCTGCATCGCTTAATTTCACCGGCAGCAGCTGGTTGTTAAAGCTATTGCCGTAGAAGATGTCAGCAAAACTCGGCGCAATCACCACTTTAAAACCGTAGTAGGTc                                                                                                                                      >  1:644479/1‑140 (MQ=255) gATTAGCTTTCACCAGCGCAAACAGTTCGTCCACTTCTGCATCGCTTAATTTCACCGGCAGCAGCTGGTTGTTAAAGCTATTGCCGTAGAAGATGTCAGCAAAACTCGGCGCAATCACCACTTTAAAACCGTAGTAGGTc                                                                                                                                      <  1:884625/140‑1 (MQ=255) gATTAGCTTTCACCAGCGCAAACAGTTCGTCCACTTCTGCATCGCTTAATTTCACCGGCAGCAGCTGGTTGTTAAAGCTATTGCCGTAGAAGATGTCAGCAAAACTCGGCGCAATCACCACTTTAAAACCGTAGTAGGTc                                                                                                                                      <  1:275260/140‑1 (MQ=255) gATTAGCTTTCACCAGCGCAAACAGTTCGTCCACTTCTGCATCGCTTAATTTCACCGGCAGCAGCTGGTTGTTAAAGCTATTGCCGTAGAAGATGTCAGCAAAACTCGGCGCAATCACCACTTTAAAACCGTAGTAGGTc                                                                                                                                      <  2:412883/140‑1 (MQ=255)   ttAGCTTTCACCAGCGCAAACAGTTCGTCCACTTCTGCATCGCTTAATTTCACCGGCAGCAGCTGGTTGTTAAAGCTATTGCCGTAGAAGATGTCAGCAAAACTCGGCGCAATCACCACTTTAAAACCGTAGTAGGTCaa                                                                                                                                    <  2:1076426/140‑1 (MQ=255)       cTTTCACCAGCGCAAACAGTTCGTCCACTTCTGCATCGCTTAATTTCACCGGCAGCAGCTGGTTGTTAAAGCTATTGCCGTAGAAGATGTCAGCAAAACTCGGCGCAATCACCACTTTAAAACCGTAGTAGGTCAATGcc                                                                                                                                >  2:45449/1‑140 (MQ=255)         ttCACCAGCGCAAACAGTTCGTCCACTTCTGCATCGCTTAATTTCACCGGCAGCAGCTGGTTGTTAAAGCTATTGCCGTAGAAGATGTCAGCAAAACTCGGCGCAATCACCACTTTAAAACCGTAGTAGGTCAATGccc                                                                                                                               >  1:1058272/1‑139 (MQ=255)         ttCACCAGCGCAAACAGTTCGTCCACTTCTGCATCGCTTAATTTCACCGGCAGCAGCTGGTTGTTAAAGCTATTGCCGTAGAAGATGTCAGCAAAACTCGGCGCAATCACCACTTTAAAACCGTAGTAGGTCAATGccc                                                                                                                               <  2:1058272/139‑1 (MQ=255)                gcgcAAACAGTTCGTCCACTTCTGCATCGCTTAATTTCACCGGCAGCAGCTGGTTGTTAAAGCTATTGCCGTAGAAGATGTCAGCAAAACTCGGCGCAATCACCACTTTAAAACCGTAGTAGGTCAATGCCCAGGGcgcg                                                                                                                       <  2:152042/140‑1 (MQ=255)                           tCGTCCACTTCTGCATCGCTTAATTTCACCGGCAGCAGCTGGTTGTTAAAGCTATTGCCGTAGAAGATGTCAGCAAAACTCGGCGCAATCACCACTTTAAAACCGTAGTAGGTCAATGCCCAGGGCGCGTGCTCACGCGa                                                                                                            >  1:132461/1‑140 (MQ=255)                            cGTCCACTTCTGCATCGCTTAATTTCACCGGCAGCAGCTGGTTGTTAAAGCTATTGCCGTAGAAGATGTCAGCAAAACTCGGCGCAATCACCACTTTAAAACCGTAGTAGGTCAATGCCCAGGGCGCGTGCTCACGCGaa                                                                                                           >  1:812970/1‑140 (MQ=255)                                    ccTGCATCGCTTAATTTCACCGGCAGCAGCTGGTTGTTAAAGCTATTGCCGTAGAAGATGTCAGCAAAACTCGGCGCAATCACCACTTTAAAACCGTAGTAGGTCAATGCCCAGGGCGCGTGCTCACGCGAAGAGCcaca                                                                                                   >  2:291851/2‑140 (MQ=255)                                           cGCTTAATTTCACCGGCAGCAGCTGGTTGTTAAAGCTATTGCCGTAGAAGATGTCAGCAAAACTCGGCGCAATCACCACTTTAAAACCGTAGTAGGTCAATGCCCAGGGCGCGTGCTCACGCGAAGAGCCACAGCCGAAg                                                                                            >  1:47895/1‑140 (MQ=255)                                             cTTAATTTCACCGGCAGCAGCTGGTTGTTAAAGCTATTGCCGTAGAAGATGTCAGCAAAACTCGGCGCAATCACCACTTTAAAACCGTAGTAGGTCAATGCCCAGGGCGCGTGCTCACGCGAAGAGCCACAGCCGAAGtt                                                                                          >  2:142129/1‑140 (MQ=255)                                             cTTAATTTCACCGGCAGCAGCTGGTTGTTAAAGCTATTGCCGTAGAAGATGTCAGCAAAACTCGGCGCAATCACCACTTTAAAACCGTAGTAGGTCAATGCCCAGGGCGCGTGCTCACGCGAAGAGCCACAGCCGAAGtt                                                                                          >  2:450604/1‑140 (MQ=255)                                             cTTAATTTCACCGGCAGCAGCTGGTTGTTAAAGCTATTGCCGTAGAAGATGTCAGCAAAACTCGGCGCAATCACCACTTTAAAACCGTAGTAGGTCAATGCCCAGGGCGCGTGCTCACGCGAAGAGCCACAGCCGAAGtt                                                                                          >  1:594222/1‑140 (MQ=255)                                                  tttCACCGGCAGCAGCTGGTTGTTAAAGCTATTGCCGTAGAAGATGTCAGCAAAACTCGGCGCAATCACCACTTTAAAACCGTAGTAGGTCAATGCCCAGGGCGCGTGCTCACGCGAAGAGCCACAGCCGAAGTTTtctc                                                                                     >  2:958883/1‑140 (MQ=255)                                                     cACCGGCAGCAGCTGGTTGTTAAAGCTATTGCCGTAGAAGATGTCAGCAAAACTCGGCGCAATCACCACTTTAAAACCGTAGTAGGTCAATGCCCAGGGCGCGTGCTCACGCGAAGAGCCACAGCCGAAGTTTTCTCGTg                                                                                  >  2:103504/1‑140 (MQ=255)                                                       ccGGCAGCAGCTGGTTGTTAAAGCTATTGCCGTAGAAGATGTCAGCAAAACTCGGCGCAATCACCACTTTAAAACCGTAGTAGGTCAATGCCCAGGGCGCGTGCTCACGCGAAGAGCCACAGCCGAAGTTTTCTCGTGcc                                                                                >  1:88720/1‑140 (MQ=255)                                                       ccGGCAGCAGCTGGTTGTTAAAGCTATTGCCGTAGAAGATGTCAGCAAAACTCGGCGCAATCACCACTTTAAAACCGTAGTAGGTCAATGCCCAGGGCGCGTGCTCACGCGAAGAGCCACAGCCGAAGTTTTCTCGTGcc                                                                                >  1:492174/1‑140 (MQ=255)                                                       ccGGCAGCAGCTGGTTGTTAAAGCTATTGCCGTAGAAGATGTCAGCAAAACTCGGCGCAATCACCACTTTAAAACCGTAGTAGGTCAATGCCCAGGGCGCGTGCTCACGCGAAGAGCCACAGCCGAAGTTTTCTCGTGcc                                                                                >  2:81922/1‑140 (MQ=255)                                                        cGGCAGCAGCTGGTTGTTAAAGCTATTGCCGTAGAAGATGTCAGCAAAACTCGGCGCAATCACCACTTTAAAACCGTAGTAGGTCAATGCCCAGGGCGCGTGCTCACGCGAAGAGCCACAGCCGAAGTTTTCTCGTGCCa                                                                               >  1:157229/1‑140 (MQ=255)                                                         ggCAGCAGCTGGTTGTTAAAGCTATTGCCGTAGAAGATGTCAGCAAAACTCGGCGCAATCACCACTTTAAAACCGTAGTAGGTCAATGCCCAGGGCGCGTGCTCACGCGAAGAGCCACAGCCGAAGTTTTCTCGTGCCAg                                                                              <  2:818488/140‑1 (MQ=255)                                                                gcTGGTTGTTAAAGCTATTGCCGTAGAAGATGTCAGCAAAACTCGGCGCAATCACCACTTTAAAACCGTAGTAGGTCAATGCCCAGGGCGCGTGCTCACGCGAAGAGCCACAGCCGAAGTTTTCTCGTGcc                                                                                >  1:978068/1‑131 (MQ=255)                                                                gcTGGTTGTTAAAGCTATTGCCGTAGAAGATGTCAGCAAAACTCGGCGCAATCACCACTTTAAAACCGTAGTAGGTCAATGCCCAGGGCGCGTGCTCACGCGAAGAGCCACAGCCGAAGTTTTCTCGTGcc                                                                                <  2:978068/131‑1 (MQ=255)                                                                  tGGTTGTTAAAGCTATTGCCGTAGAAGATGTCAGCAAAACTCGGCGCAATCACCACTTTAAAACCGTAGTAGGTCAATGCCCAGGGCGCGTGCTCACGCGAAGAGCCACAGCCGAAGTTTTCTCGTGCCAGCAAAATGGa                                                                     <  2:47895/140‑1 (MQ=255)                                                                  tGGTTGTTAAAGCTATTGCCGTAGAAGATGTCAGCAAAACTCGGCGCAATCACCACTTTAAAACCGTAGTAGGTCAATGCCCAGGGCGCGTGCTCACGCGAAGAGCCACAGCCGAAGTTTTCTCGTGCCAGCAAAATGGa                                                                     <  2:731512/140‑1 (MQ=255)                                                                  tGGTTGTTAAAGCTATTGCCGTAGAAGATGTCAGCAAAACTCGGCGCAATCACCACTTTAAAACCGTAGTAGGTCAATGCCCAGGGCGCGTGCTCACGCGAAGAGCCACAGCCGAAGTTTTCTCGTGCCAGCAAAATGGa                                                                     <  2:132461/140‑1 (MQ=255)                                                                  tGGTTGTTAAAGCTATTGCCGTAGAAGATGTCAGCAAAACTCGGCGCAATCACCACTTTAAAACCGTAGTAGGTCAATGCCCAGGGCGCGTGCTCACGCGAAGAGCCACAGCCGAAGTTTTCTCGTGCCAGCAAAATGGa                                                                     <  1:691981/140‑1 (MQ=255)                                                                    gttgttAAAGCTATTGCCGTAGAAGATGTCAGCAAAACTCGGCGCAATCACCACTTTAAATCCGTAGTAGGTCAATGCCCAGGGCGCGTGCTCACGCGAAGAGCCACAGCCGAAGTTTTCTCGTGCCAGCAAAATGGAAg                                                                   <  1:463602/140‑1 (MQ=255)                                                                    gttgttAAAGCTATTGCCGTAGAAGATGTCAGCAAAACTCGGCGCAATCACCACTTTAAAACCGTAGTAGGTCAATGCCCAGGGCGCGTGCTCACGCGAAGAGCCACAGCCGAAGTTTTCTCGTGCCAGCAAAATGGAAg                                                                   <  1:45449/140‑1 (MQ=255)                                                                    gttgttAAAGCTATTGCCGTAGAAGATGTCAGCAAAACTCGGCGCAATCACCACTTTAAAACCGTAGTAGGTCAATGCCCAGGGCGCGTGCTCACGCGAAGAGCCACAGCCGAAGTTTTCTCGTGCCAGCAAAATGGAAg                                                                   <  2:406143/140‑1 (MQ=255)                                                                    gttgttAAAGCTATTGCCGTAGAAGATGTCAGCAAAACTCGGCGCAATCACCACTTTAAAACCGTAGTAGGTCAATGCCCAGGGCGCGTGCTCACGCGAAGAGCCACAGCCGAAGTTTTCTCGTGCCAGCAAAATGGAAg                                                                   <  2:644479/140‑1 (MQ=255)                                                                    gttgttAAAGCTATTGCCGTAGAAGATGTCAGCAAAACTCGGCGCAATCACCACTTTAAAACCGTAGTAGGTCAATGCCCAGGGCGCGTGCTCACGCGAAGAGCCACAGCCGAAGTTTTCTCGTGCCAGCAAAATGGAAg                                                                   <  2:492174/140‑1 (MQ=255)                                                                    gttgttAAAGCTATTGCCGTAGAAGATGTCAGCAAAACTCGGCGCAATCACCACTTTAAAACCGTAGTAGGTCAATGCCCAGGGCGCGTGCTCACGCGAAGAGCCACAGCCGAAGTTTTCTCGTGCCAGCAAAATGGAAg                                                                   <  2:299308/140‑1 (MQ=255)                                                                    gttgttAAAGCTATTGCCGTAGAAGATGTCAGCAAAACTCGGCGCAATCACCACTTTAAAACCGTAGTAGGTCAATGCCCAGGGCGCGTGCTCACGCGAAGAGCCACAGCCGAAGTTTTCTCGTGCCAGCAAAATGGAAg                                                                   <  1:81922/140‑1 (MQ=255)                                                                    gttgttAAAGCTATTGCCGTAGAAGATGTCAGCAAAACTCGGCGCAATCACCACTTTAAAACCGTAGTAGGTCAATGCCCAGGGCGCGTGCTCACGCGAAGAGCCACAGCCGAAGTTTTCTCGTGCCAGCAAAATGGAAg                                                                   <  2:88720/140‑1 (MQ=255)                                                                    gttgttAAAGCTATTGCCGTAGAAGATGTCAGCAAAACTCGGCGCAATCACCACTTTAAAACCGTAGTAGGTCAATGCCCAGGGCGCGTGCTCACGCGAAGAGCCACAGCCGAAGTTTTCTCGTGCCAGCAAAATGGAAg                                                                   <  1:703518/140‑1 (MQ=255)                                                                           aaGCTATTGCCGTAGAAGATGTCAGCAAAACTCGGCGCAATCACCACTTTAAAACCGTAGTAGGTCAATGCCCAGGGCGCGTGCTCACGCGAAGAGCCACAGCCGAAGTTTTCTCGTGCCAGCAAAATGGAAGCGCCCTg                                                            >  2:47710/1‑140 (MQ=255)                                                                              cTATTGCCGTAGAAGATGTCAGCAAAACTCGGCGCAATCACCACTTTAAAACCGTAGTAGGTCAATGCCCAGGGCGCGTGCTCACGCGAAGAGCCACAGCCGAAGTTTTCTCGTGCCAGCAAAATGGAAGCGCCCTGATa                                                         <  1:47710/140‑1 (MQ=255)                                                                                 ttGCCGTAGAAGATGTCAGCAAAACTCGGCGCAATCACCACTTTAAAACCGTAGTAGGTCAATGCCCAGGGCGCGTGCTCACGCGAAGAGCCACAGCCGAAGTTTTCTCGTGCCAGCAAAATGGa                                                                     >  1:929766/1‑125 (MQ=255)                                                                                 ttGCCGTAGAAGATGTCAGCAAAACTCGGCGCAATCACCACTTTAAAACCGTAGTAGGTCAATGCCCAGGGCGCGTGCTCACGCGAAGAGCCACAGCCGAAGTTTTCTCGTGCCAGCAAAATGGa                                                                     <  2:929766/125‑1 (MQ=255)                                                                                     cGTAGAAGATGTCAGCAAAACTCGGCGCAATCACCACTTTAAAACCGTAGTAGGTCAATGCCCAGGGCGCGTGCTCACGCGAAGAGCCACAGCCGAAGTTTTCTCGTGCCAGCAAAATGGAAGCGCCCTGATACTGCggg                                                  <  2:157229/140‑1 (MQ=255)                                                                                       tAGAAGATGTCAGCAAAACTCGGCGCAATCACCACTTTAAAACCGTAGTAGGTCAATGCCCAGGGCGCGTGCTCACGCGAAGAGCCACAGCCGAAGTTTTCTCGTGCCAGCAAAATGGAAGCGCCCTGATACTGCGGGaa                                                >  2:339671/1‑140 (MQ=255)                                                                                         gaagaTGTCAGCGAAACTCGGCGCAATCACCACTTTAAAACCGTAGTAGGTCAATGCCCAGGGCGCGTGCTCACGCGAAGAGCCACAGCCGAAGTTTTCTCGTGCCAGCAAAATGGAAGCGCCCTGATACTGCGGGAAGt                                              <  2:277866/140‑1 (MQ=255)                                                                                            gaTGTCAGCAAAACTCGGCGCAATCACCACTTTAAAACCGTAGTAGGTCAATGCCCAGGGCGCGTGCTCACGCGAAGAGCCACAGCCGAAGTTTTCTCGTGCCAGCAAAATGGAAGCGCCCTGATACTGCGGGAAGTTCa                                           >  1:59248/1‑140 (MQ=255)                                                                                              tGTCAGCAAAACTCGGCGCAATCACCACTTTAAAACCGTAGTAGGTCAATGCCCAGGGCGCGTGCTCACGCGAAGAGCCACAGCCGAAGTTTTCTCGTGCCAGCAAAATGGAAGCGCCCTGATACTGCGGGAAGTTCAGc                                         >  1:320737/1‑140 (MQ=255)                                                                                               gTCAGCAAAACTCGGCGCAATCACCACTTTAAAACCGTAGTAGGTCAATGCCCAGGGCGCGTGCTCACGCGAAGAGCCACAGCCGAAGTTTTCTCGTGCCAGCAAAATGGAAGCGCCCTGATACTGCGGGAAGTTCAGCa                                        <  2:1074062/140‑1 (MQ=255)                                                                                                 cAGCAAAACTCGGCGCAATCACCACTTTAAAACCGTAGTAGGTCAATGCCCAGGGCGCGTGCTCACGCGAAGAGCCACAGCCGAAGTTTTCTCGTGCCAGCAAAATGGAAGCGCCCTGATACTGCGGGAAGTTCAGCACg                                      <  1:291851/140‑1 (MQ=255)                                                                                                 cAGCAAAACTCGGCGCAATCACCACTTTAAAACCGTAGTAGGTCAATGCCCAGGGCGCGTGCTCACGCGAAGAGCCACAGCCGAAGTTTTCTCGTGCCAGCAAAATGGAAGCGCCCTGATACTGCGGGAAGTTCAGCACg                                      <  1:103504/140‑1 (MQ=255)                                                                                                 cAGCAAAACTCGGCGCAATCACCACTTTAAAACCGTAGTAGGTCAATGCCCAGGGCGCGTGCTCACGCGAAGAGCCACAGCCGAAGTTTTCTCGTGCCAGCAAAATGGAAGCGCCCTGATACTGCGGGAAGTTCAGCACg                                      <  1:958883/140‑1 (MQ=255)                                                                                                 cAGCAAAACTCGGCGCAATCACCACTTTAAAACCGTAGTAGGTCAATGCCCAGGGCGCGTGCTCACGCGAAGAGCCACAGCCGAAGTTTTCTCGTGCCAGCAAAATGGAAGCGCCCTGATACTGCGGGAAGTTCAGCACg                                      <  1:142129/140‑1 (MQ=255)                                                                                                  aGCAAAACTCGGCGCAATCACCACTTTAAAACCGTAGTAGGTCAATGCCCAGGGCGCGTGCTCACGCGAAGAGCCACAGCCGAAGTTTTCTCGTGCCAGCAAAATGGAAGCGCCCTGATACTGCGGGAAGTTCAGCACGa                                     >  2:544699/1‑140 (MQ=255)                                                                                                  aGCAAAACTCGGCGCAATCACCACTTTAAAACCGTAGTAGGTCAATGCCCAGGGCGCGTGCTCACGCGAAGAGCCACAGCCGAAGTTTTCTCGTGCCAGCAAAATGGAAGCGCCCTGATACTGCGGGAAGTTCAGCACGa                                     >  2:375143/1‑140 (MQ=255)                                                                                                    cAAAACTCGGCGCAATCACCACTTTAAAACCGTAGTAGGTCAATGCCCAGGGCGCGTGCTCACGCGAAGAGCCACAGCCGAAGTTTTCTCGTGCCAGCAAAATGGAAGCGCCCTGATACTGCGGGAAGTTCAGCACGAAg                                   >  1:55263/1‑140 (MQ=255)                                                                                                    cAAAACTCGGCGCAATCACCACTTTAAAACCGTAGTAGGTCAATGCCCAGGGCGCGTGCTCACGCGAAGAGCCACAGCCGAAGTTTTCTCGTGCCAGCAAAATGGAAGCGCCCTGATACTGCGGGAAGTTCAGCACGAAg                                   >  1:873699/1‑140 (MQ=255)                                                                                                    cAAAACTCGGCGCAATCACCACTTTAAAACCGTAGTAGGTCAATGCCCAGGGCGCGTGCTCACGCGAAGAGCCACAGCCGAAGTTTTCTCGTGCCAGCAAAATGGAAGCGCCCTGATACTGCGGGAAGTTCAGCACGAAg                                   >  1:737679/1‑140 (MQ=255)                                                                                                     aaaaCTCGGCGCAATCACCACTTTAAAACCGTAGTAGGTCAATGCCCAGGGCGCGTGCTCACGCGAAGAGCCACAGCCGAAGTTTTCTCGTGCCAGCAAAATGGAAGCGCCCTGATACTGCGGGAAGTTCAGCACGAAGt                                  <  1:544699/140‑1 (MQ=255)                                                                                                       aaCTCGGCGCAATCACCACTTTAAAACCGTAGTAGGTCAATGCCCAGGGCGCGTGCTCACGCGAAGAGCCACAGCCGAAGTTTTCTCGTGCCAGCAAAATGGAAGCGCCCTGATACTGCGGGAAGTTCAGCACGAAGTcc                                <  2:594222/140‑1 (MQ=255)                                                                                                         cTCGGCGCAATCACCACTTTAAAACCGTAGTAGGTCAATGCCCAGGGCGCGTGCTCACGCGAAGAGCCACAGCCGAAGTTTTCTCGTGCCAGCAAAATGGAAGCGCCCTGATACTGCGGGAAGTTCAGCACGAAGTCCgg                              >  1:116577/1‑140 (MQ=255)                                                                                                              cgcAATCACCACTTTAAAACCGTAGTAGGTCAATGCCCAGGGCGCGTGCTCACGCGAAGAGCCACAGCCGAAGTTTTCTCGTGCCAGCAAAATGGAAGCGCCCTGATACTGCGGGAAGTTCAGCACGAAGTCCGGGTTTg                         >  2:121515/1‑140 (MQ=255)                                                                                                                  aTCACCACTTTAAAACCGTAGTAGGTCAATGCCCAGGGCGCGTGCTCACGCGAAGAGCCACAGCCGAAGTTTTCTCGTGCCAGCAAAATGGAAGCGCCCTGATACTGCGGGAAGTTCAGCACGAAGTCCGGGTTTGGCTg                     <  1:278269/140‑1 (MQ=255)                                                                                                                   tCACCACTTTAAAACCGTAGTAGGTCAATGCCCAGGGCGCGTGCTCACGCGAAGAGCCACAGCCGAAGTTTTCTCGTGCCAGCAAAATGGAAGCGCCCTGATACTGCGGGAAGTTCAGCACGAAGTCCGGGTTTGGCTGt                    <  2:320737/140‑1 (MQ=255)                                                                                                                       cgcTTTAAAACCGTAGTAGGTCAATGCCCAGGGCGCGTGCTCACGCGAAGAGCCACAGCCGAAGTTTTCTCGTGCCAGCAAAATGGAAGCGCCCTGATACTGCGGGAAGTTCAGCACGAAGTCCGGGTTTGGCTGTTGGc                <  2:812970/138‑1 (MQ=255)                                                                                                                            tAAAACCGTAGTAGGTCAATGCCCAGGGCGCGTGCTCACGCGAAGAGCCACAGCCGAAGTTTTCTCGTGCCAGCAAAATGGAAGCGCCCTGATACTGCGGGAAGTTCAGCACGAAGTCCGGGTTTGGCTGTTGGCCtttt           >  2:232591/1‑140 (MQ=255)                                                                                                                              aaaCCGTAGTAGGTCAATGCCCAGGGCGCGTGCTCACGCGAAGAGCCACAGCCGAAGTTTTCTCGTGCCAGCAAAATGGAAGCGCCCTGATACTGCGGGAAGTTCAGCACGAAGTCCGGGTTTGGCTGTTGGCCTTTTTc         >  1:798496/1‑140 (MQ=255)                                                                                                                                aCCGTAGTAGGTCAATGCCCAGGGCGCGTGCTCACGCGAAGAGCCACAGCCGAAGTTTTCTCGTGCCAGCAAAATGGAAGCGCCCTGATACTGCGGGAAGTTCAGCACGAAGTCCGGGTTTGGCTGTTGGCCTTTTTCAt       <  1:450604/140‑1 (MQ=255)                                                                                                                                    tagtagGTCAATGCCCAGGGCGCGTGCTCACGCGAAGAGCCACAGCCGAAGTTTTCTCGTGCCAGCAAAATGGAAGCGCCCTGATACTGCGGGAAGTTCAGCACGAAGTCCGGGTTTGGCTGTTGGCCTTTTTCATCCAg   >  1:61866/1‑140 (MQ=255)                                                                                                                                     aGTAGGTCAATGCCCAGGGCGCGTGCTCACGCGAAGAGCCACAGCCGAAGTTTTCTCGTGCCAGCAAAATGGAAGCGCCCTGATACTGCGGGAAGTTCAGCACGAAGTCCGGGTTTGGCTGTTGGCCTTTTTCATCCAGa  >  2:542084/1‑140 (MQ=255)                                                                                                                                        |                                                                                                                                         GATTAGCTTTCACCAGCGCAAACAGTTCGTCCACTTCTGCATCGCTTAATTTCACCGGCAGCAGCTGGTTGTTAAAGCTATTGCCGTAGAAGATGTCAGCAAAACTCGGCGCAATCACCACTTTAAAACCGTAGTCGGTCAATGCCCAGGGCGCGTGCTCACGCGAAGAGCCACAGCCGAAGTTTTCTCGTGCCAGCAAAATGGAAGCGCCCTGATACTGCGGGAAGTTCAGCACGAAGTCCGGGTTTGGCTGTTGGCCTTTTTCATCCAGA  >  NC_000913/79036‑79307

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 23 ≤ ATCG/ATCG < 30 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑