Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	3,859,407	C→A	G237V (GGC→GTC)	yidK ←	putative transporter YidK

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	3,859,407	0	C	A	100.0%	127.1 / NA	36	G237V (GGC→GTC)	yidK	putative transporter YidK
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base A (18/18);  total (18/18)

CGCCCCCTTTTGCCCTTCCGCCAGGCTTTTTGACGCCAGCGTGCGTTGCACGATGCCCTGATTTGTACACCAGTAAAAGGTGTTCACCAGAATCAAACCGGTAAATGCCGCGCCAATCGGCAAGGGATCGGTCGGGCCACCGATTGAGTTTAATTTCTCGGCGTGAACGGTGGTGAGTTGCTCAATGCCCTGCATAAAGCTGCCCTTGCCCATCGCGATTAGGCCAAATACCGGCACCATCAACC  >  NC_000913/3859271‑3859515                                                                                                                                         |                                                                                                             cgcCCCCTTTTGCCCTTCCGCCAGGCTTTTTGACGCCAGCGTGCGTTGCACGATGCCCTGATTTGTACACCAGTAAAAGGTGTTCACCAGAATCAAACCGGTAAATGCCGCGCCAATCGGCAAGGGATCGGTCGGGacac                                                                                                           >  2:806684/1‑140 (MQ=255) cgcCCCCTTTTGCCCTTCCGCCAGGCTTTTTGACGCCAGCGTGCGTTGCACGATGCCCTGATTTGTACACCAGTAAAAGGTGTTCACCAGAATCAAACCGGTAAATGCCGCGCCAATCGGCAAGGGATCGGTCGGGacac                                                                                                           >  2:207186/1‑140 (MQ=255) cgcCCCCTTTTGCCCTTCCGCCAGGCTTTTTGACGCCAGCGTGCGTTGCACGATGCCCTGATTTGTACACCAGTAAAAGGTGTTCACCAGAATCAAACCGGTAAATGCCGCGCCAATCGGCAAGGGATCGGTCGGGacac                                                                                                           >  1:791747/1‑140 (MQ=255) cgcCCCCTTTTGCCCTTCCGCCAGGCTTTTTGACGCCAGCGTGCGTTGCACGATGCCCTGATTTGTACACCAGTAAAAGGTGTTCACCAGAATCAAACCGGTAAATGCCGCGCCAATCGGCAAGGGATCGGTCGGGACa                                                                                                            >  2:167479/1‑139 (MQ=255)  gcCCCCTTTTGCCCTTCCGCCAGGCTTTTTGACGCCAGCGTGCGTTGCACGATGCCCTGATTTGTACACCAGTAAAAGGTGTTCACCAGAATCAAACCGGTAAATGCCGCGCCAATCGGCAAGGGATCGGTCGGGACAcc                                                                                                          >  2:8107/1‑140 (MQ=255)    ccccTTTTGCCCTTCCGCCAGGCTTTTTGACGCCAGCGTGCGTTGCACGATGCCCTGATTTGTACACCAGTAAAAGGTGTTCACCAGAATCAAACCGGTAAATGCCGCGCCAATCGGCAAGGGATCGGTCGGGACACCGa                                                                                                        >  1:1146538/1‑140 (MQ=255)    ccccTTTTGCCCTTCCGCCAGGCTTTTTGACGCCAGCGTGCGTTGCACGATGCCCTGATTTGTACACCAGTAAAAGGTGTTCACCAGAATCAAACCGGTAAATGCCGCGCCAATCGGCAAGGGATCGGTCGGGACACCGa                                                                                                        <  1:477440/140‑1 (MQ=255)     cccTTTTGCCCTTCCGCCAGGCTTTTTGACGCCAGCGTGCGTTGCACGATGCCCTGATTTGTACACCAGTAAAAGGTGTTCACCAGAATCAAACCGGTAAATGCCGCGCCAATCGGCAAGGGATCGGTCGGGACACCGAt                                                                                                       <  1:731088/140‑1 (MQ=255)      ccTTTTGCCCTTCCGCCAGGCTTTTTGACGCCAGCGTGCGTTGCACGATGCCCTGATTTGTACACCAGTAAAAGGTGTTCACCAGAATCAAACCGGTAAATGCCGCGCCAATCGGCAAGGGATCGGTCGGGACACCGAtt                                                                                                      <  2:533591/140‑1 (MQ=255)                  ccGCCAGGCTTTTTGACGCCAGCGTGCGTTGCACGATGCCCTGATTTGTACACCAGTAAAAGGTGTTCACCAGAATCAAACCGGTAAATGCCGCGCCAATCGGCAAGGGATCGGTCGGGACACCGATTGAGTTTAATTtc                                                                                          >  1:561787/1‑140 (MQ=255)                            ttttGACGCCAGCGTGCGTTGCACGATGCCCTGATTTGTACACCAGTAAAAGGTGTTCACCAGAATCAAACCGGTAAATGCCGCGCCAATCGGCAAGGGATCGGTCGGGACACCGATTGAGTTTAATTTCTCGGCGTGaa                                                                                <  1:8107/140‑1 (MQ=255)                            ttttGACGCCAGCGTGCGTTGCACGATGCCCTGATTTGTACACCAGTAAAAGGTGTTCACCAGAATCAAACCGGTAAATGCCGCGCCAATCGGCAAGGGATCGGTCGGGACACCGATTGAGTTTAATTTCTCGGCGTGaa                                                                                <  2:259052/140‑1 (MQ=255)                            ttttGACGCCAGCGTGCGTTGCACGATGCCCTGATTTGTACACCAGTAAAAGGTGTTCACCAGAATCAAACCGGTAAATGCCGCGCCAATCGGCAAGGGATCGGTCGGGACACCGATTGAGTTTAATTTCTCGGCGTGaa                                                                                <  2:162595/140‑1 (MQ=255)                            ttttGACGCCAGCGTGCGTTGCACGATGCCCTGATTTGTACACCAGTAAAAGGTGTTCACCAGAATCAAACCGGTAAATGCCGCGCCAATCGGCAAGGGATCGGTCGGGACACCGATTGAGTTTAATTTCTCGGCGTGaa                                                                                <  1:806684/140‑1 (MQ=255)                                             gtTGCACGATGCCCTGATTTGTACACCAGTAAAAGGTGTTCACCAGAATCAAACCGGTAAATGCCGCGCCAATCGGCAAGGGATCGGTCGGGACACCGATTGAGTTTAATTTCTCGGCGTGAACGGTGGTGAGTTGCTCa                                                               <  1:207186/140‑1 (MQ=255)                                                  aCGATGCCCTGATTTGTACACCAGTAAAAGGTGTTCACCAGAATCAAACCGGTAAATGCCGCGCCAATCGGCAAGGGATCGGTCGGGACACCGATTGAGTTTAATTTCTCGGCGTGAACGGTGGTGAGTTGCTCAATGcc                                                          <  2:497786/140‑1 (MQ=255)                                                    gATGCCCTGATTTGTACACCAGTAAAAGGTGTTCACCAGAATCAAACCGGTAAATGCCGCGCCAATCGGCAAGGGATCGGTCGGGACACCGATTGAGTTTAATTTCTCGGCGTGAACGGTGGTGAGTTGCTCAATGcc                                                          <  1:611900/138‑1 (MQ=255)                                                    gATGCCCTGATTTGTACACCAGTAAAAGGTGTTAACCAGAATCAAACCGGTAAATGCCGCGCCAATCGGCAAGGGATCGGTCGGGACACCGATTGAGTTTAATTTCTCGGCGTGAACGGTGGTGAGTTGCTCAATGcc                                                          >  2:611900/1‑138 (MQ=255)                                                            gATTTGTACACCAGTAAAAGGTGTTCACCAGAATCAAACCGGTAAATGCCGCGCCAATCGGCAAGGGATCGGTCGGGACACCGATTGAGTTTAATTTCTCGGCGTGAACGGTGGTGAGTTGCTCAATGCCCTGCATAAAg                                                <  2:1101599/140‑1 (MQ=255)                                                                   acacCAGTAAAAGGTGTTCACCAGAATCAAACCGGTAAATGCCGCGCCAATCGGCAAGGGATCGGTCGGGACACCGATTGAGTTTAATTTCTCGGCGTGAACGGTGGTGAGTTGCTCAATGCCCTGCATAAAGCTGCCCt                                         >  1:1122418/1‑140 (MQ=255)                                                                       cAGTAAAAGGTGTTCACCAGAATCAAACCGGTAAATGCCGCGCCAATCGGCAAGGGATCGGTCGGGACACCGATTGAGTTTAATTTCTCGGCGTGAACGGTGGTGAGTTGCTCAATGCCCTGCATAAAGCTGCCCTTGcc                                     >  2:582068/1‑140 (MQ=255)                                                                          tAAAAGGTGTTCACCAGAATCAAACCGGTAAATGCCGCGCCAATCGGCAAGGGATCGGTCGGGACACCGATTGAGTTTAATTTCTCGGCGTGAACGGTGGTGAGTTGCTCAATGCCCTGCATAAAGCTGCCCTTGCCCAt                                  >  1:235314/1‑140 (MQ=255)                                                                          tAAAAGGTGTTCACCAGAATCAAACCGGTAAATGCCGCGCCAATCGGCAAGGGATCGGTCGGGACACCGATTGAGTTTAATTTCTCGGCGTGAACGGTGGTGAGTTGCTCAATGCCCTGCATAAAGCTGCCCTTGCCCAt                                  >  1:1197647/1‑140 (MQ=255)                                                                            aaaGGTGTTCACCAGAATCAAACCGGTAAATGCCGCGCCAATCGGCAAGGGATCGGTCGGGACACCGATTGAGTTTAATTTCTCGGCGTGAACGGTGGTGAGTTGCTCAATGCCCTGCATAAAGCTGCCCTTGCCCATcg                                >  1:361371/1‑140 (MQ=255)                                                                                  gtTCACCAGAATCAAACCGGTAAATGCCGCGCCAATCGGCAAGGGATCGGTCGGGACACCGATTGAGTTTAATTTCTCGGCGTGAACGGTGGTGAGTTGCTCAATGCCCTGCATAAAGCTGCCCTTGCCCATCGCGATTa                          >  2:685742/1‑140 (MQ=255)                                                                                  gtTCACCAGAATCAAACCGGTAAATGCCGCGCCAATCGGCAAGGGATCGGTCGGGACACCGATTGAGTTTAATTTCTCGGCGTGAACGGTGGTGAGTTGCTCAATGCCCTGCATAAAGCTGCCCTTGCCCATCGCGATTa                          <  2:1122418/140‑1 (MQ=255)                                                                                  gtTCACCAGAATCAAACCGGTAAATGCCGCGCCAATCGGCAAGGGATCGGTCGGGACACCGATTGAGTTTAATTTCTCGGCGTGAACGGTGGTGAGTTGCTCAATGCCCTGCATAAAGCTGCCCTTGCCCATCGCGATTa                          >  2:508185/1‑140 (MQ=255)                                                                                   ttCACCAGAATCAAACCGGTAAATGCCGCGCCAATCGGCAAGGGATCGGTCGGGACACCGATTGAGTTTAATTTCTCGGCGTGAACGGTGGTGAGGTGCTCAATGCCCTGCATAAAGCTGCCCTTGCCCATCGCGATTAg                         >  1:197080/1‑140 (MQ=255)                                                                                   ttCACCAGAATCAAACCGGTAAATGCCGCGCCAATCGGCAAGGGATCGGTCGGGACACCGATTGAGTTTAATTTCTCGGCGTGAACGGTGGCGAGTTGCTCAATGCCCTGCATAAAGCTGCCCTTGCCCATCGCGATTAg                         >  1:224974/1‑140 (MQ=255)                                                                                    tCACCAGAATCAAACCGGTAAATGCCGCGCCAATCGGCAAGGGATCGGTCGGGACACCGATTGAGTTTAATTTCTCGGCGTGAACGGTGGTGAGTTGCTCAATGCCCTGCATAAAGCTGCCCTTGCCCATCGCGATTa                          >  2:133976/1‑138 (MQ=255)                                                                                    tCACCAGAATCAAACCGGTAAATGCCGCGCCAATCGGCAAGGGATCGGTCGGGACACCGATTGAGTTTAATTTCTCGGCGTGAACGGTGGTGAGTTGCTCAATGCCCTGCATAAAGCTGCCCTTGCCCATCGCGATTa                          <  1:133976/138‑1 (MQ=255)                                                                                       ccAGAATCAAACCGGTAAATGCCGCGCCAATCGGCAAGGGATCGGTCGGGACACCGATTGAGTTTAATTTCTCGGCGTGAACGGTGGTGAGTTGCTCAATGCCCTGCATAAAGCTGCCCTTGCCCATCGCGATTAGGCCa                     <  2:928600/140‑1 (MQ=255)                                                                                               aaaCCGGTAAATGCCGCGCCAATCGGCAAGGGATCGGTCGGGACACCGATTGAGTTTAATTTCTCGGCGTGAACGGTGGTGAGTTGCTCAATGCCCTGCATAAAGCTGCCCTTGCCCATCGCGATTAGGTCAAATACCgg             <  1:582068/140‑1 (MQ=255)                                                                                               aaaCCGGTAAATGCCGCGCCAATCGGCAAGGGATCGGTCGGGACACCGATTGAGTTTAATTTCTCGGCGTGAACGGTGGTGAGTTGCTCAATGCCCTGCATAAAGCTGCCCTTGCCCATCGCGATTAGGCCAAATACCgg             <  2:1069929/140‑1 (MQ=255)                                                                                               aaaCCGGTAAATGCCGCGCCAATCGGCAAGGGATCGGTCGGGACACCGATTGAGTTTAATTTCTCGGCGTGAACGGTGGTGAGTTGCTCAATGCCCTGCATAAAGCTGCCCTTGCCCATCGCGATTAGGCCAAATACCgg             <  1:508185/140‑1 (MQ=255)                                                                                               aaaCCGGTAAATGCCGCGCCAATCGGCAAGGGATCGGTCGGGACACCGATTGAGTTTAATTTCTCGGCGTGAACGGTGGTGAGTTGCTCAATGCCCTGCATAAAGCTGCCCTTGCCCATCGCGATTAGGCCAAATACCgg             <  1:685742/140‑1 (MQ=255)                                                                                                aaCCGGTAAATGCCGCGCCAATCGGCAAGGGATCGGTCGGGACACCGATTGAGTTTAATTTCTCGGCGTGAACGGTGGTGAGTTGCTCAATGCCCTGCATAAAGCTGCCCTTGCCCATCGCGATTAGGCCAAATACCGGc            >  1:329872/1‑140 (MQ=255)                                                                                                     gTAAATGCCGCGCCAATCGGCAAGGGATCGGTCGGGACACCGATTGAGTTTAATTTCTCGGCGTGAACGGTGGTGAGTTGCTCAATGCCCTGCATAAAGCTGCCCTTGCCCATCGCGATTAGGCCAAATACCGGCACCAt       >  2:610341/1‑140 (MQ=255)                                                                                                          tGCCGCGCCAATCGGCAAGGGATCGGTCGGGACACCGATTGAGTTTAATTTCTCGGCGTGAACGGTGGTGAGTTGCTCAATGCCCTGCATAAAGCTGCCCTTGCCCATCGCGATTAGGCCAAATACCGGCACCATCAAcc  >  2:1152228/1‑140 (MQ=255)                                                                                                                                         |                                                                                                             CGCCCCCTTTTGCCCTTCCGCCAGGCTTTTTGACGCCAGCGTGCGTTGCACGATGCCCTGATTTGTACACCAGTAAAAGGTGTTCACCAGAATCAAACCGGTAAATGCCGCGCCAATCGGCAAGGGATCGGTCGGGCCACCGATTGAGTTTAATTTCTCGGCGTGAACGGTGGTGAGTTGCTCAATGCCCTGCATAAAGCTGCCCTTGCCCATCGCGATTAGGCCAAATACCGGCACCATCAACC  >  NC_000913/3859271‑3859515

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 23 ≤ ATCG/ATCG < 30 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑