Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	285,577	G→T	P61P (CCG→CCT)	yagG →	putative D‑xylonate transporter YagG

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	285,577	0	G	T	100.0%	247.8 / NA	72	P61P (CCG→CCT)	yagG	putative D‑xylonate transporter YagG
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base T (29/43);  total (29/43)

ACCGCCTGCGGCTTCGTCTGGCAGGCCACGATGTTCCTGCTGGCCTATTTCTACACCGACGTCTTCGGCCTGTCGGCGGGGATTATGGGCACGCTGTTTTTGGTCTCCCGCGTGCTCGACGCCGTCACCGACCCGCTGATGGGGCTGCTGGTAGACCGCACCCGCACGCGGCACGGCCAGTTCCGCCCGTTCCTGCTGTGGGGGGCCATCCCGTTCGGCATCGTCTGCGTGCTGACCTTCTACACGCCGGACTTCTCCGCACAGGGCAAGA  >  NC_000913/285443‑285713                                                                                                                                       |                                                                                                                                         acCGCCTGCGGCTTCGTCTGGCAGGCCACGATGTTCCTGCTGGCCTATTTCTACACCGACGTCTTCGGCCTGTCGGCGGGGATTATGGGCACGCTGTTTTTGGTCTCCCGCGTGCTCGACGCCGTCACCGACCCTCTGAt                                                                                                                                     >  2:244012/1‑140 (MQ=255) acCGCCTGCGGCTTCGTCTGGCAGGCCACGATGTTCCTGCTGGCCTATTTCTACACCGACGTCTTCGGCCTGTCGGCGGGGATTATGGGCACGCTGTTTTTGGTCTCCCGCGTGCTCGACGCCGTCACCGACCCTCTGAt                                                                                                                                     >  1:901759/1‑140 (MQ=255) acCGCCTGCGGCTTCGTCTGGCAGGCCACGATGTTCCTGCTGGCCTATTTCTACACCGACGTCTTCGGCCTGACGGCGGGGGTTATGGGCACGCTGTTTTTGGTCTCCCGCGTGCTCGACCCCGTCACCGACCCTCTGAt                                                                                                                                     >  2:1129901/1‑140 (MQ=255)  ccGCCTGCGGCTTCGTCTGGCAGGCCACGATGTTCCTGCTGGCCTATTTCTACACCGACGTCTTCGGCCTGTCGGCGGGGATTATGGGCACGCTGTTTTTGGTCTCCCGCGTGCTCGACGCCGTCACCGACCCTCTGATg                                                                                                                                    <  2:103845/140‑1 (MQ=255)      cTGCGGCTTCGTCTGGCAGGCCACGATGTTCCTGCTGGCCTATTTCTACACCGACGTCTTCGGCCTGTCGGCGGGGATTATGGGCACGCTGTTTTTGGTCTCCCGCGTGCTCGACGCCGTCACCGACCCTCTGATGGGgc                                                                                                                                >  1:665640/1‑140 (MQ=255)      cTGCGGCTTCGTCTGGCAGGCCACGATGTTCCTGCTGGCCTATTTCTACACCGACGTCTTAGGCCTGTCGGCGGGGATTATGGGCACGCTGTTTTTGGTCTCCCGCGTGCCCGACGCCGTCACCGACCCTCTGATGGGgc                                                                                                                                <  2:491611/140‑1 (MQ=255)         cGGCTTCGTCTGGCAGGCCACGATGTTCCTGCTGGCCTATTTCTACACCGACGTCTTCGGCCTGTCGGCGGGGATTATGGGCACGCTGTTTTTGGTCTCCCGCGTGCTCGACGCCGTCACCGACCCTCTGATGGGgctgc                                                                                                                             >  2:360060/1‑140 (MQ=255)                 tCTGGCAGGCCACGATGTTCCTGCTGGCCTATTTCTACACCGACGTCTTCGGCCTGTCGGCGGGGATTATGGGCACGCTGTTTTTGGTCTCCCGCGTGCTCGACGCCTTCACCGACCCTCTGATGGGGCTGCTGGTAGAc                                                                                                                     <  1:703428/140‑1 (MQ=255)                          ccACGATGTTCCTGCTGGCCTATTTCTACACCGACGTCTTCGGCCTGTCGGCGGGGATTATGGGCACGCTGTTTTTGGTCTCCCGCGTGCTCGACGCCGTCACCGACCCTCTGATGGGGCTGCTGGTAGAccgcacccgc                                                                                                            >  1:1100558/1‑140 (MQ=255)                           cACGATGTTCCTGCTGGCCTATTTCTACACCGACGTCTTCGGCCTGTCGGCGGGGGCTATGGGCCCGCTGTTTTTGGGCTCCCCCGTGCTCGCCGCCGGCACCGCCCCTCTGATGGGGCTGCCGGGAGAccgcacccgcc                                                                                                           >  2:446584/1‑139 (MQ=255)                           cACGATGTTCCTGCTGGCCTATTTCTACACCGACGTCTTCGGCCTGTCGGCGGGGATTATGGGCACGCTGTTTTTGGTCTCCCGCGTGCTCGACGCCGTCACCGACCCTCTGATGGGGCTGCTGGTAGAccgcacccgca                                                                                                           <  2:1116295/140‑1 (MQ=255)                           cACGATGTTCCTGCTGGCCTATTTCTACACCGACGTCTTCGGCCTGTCGGCGGGGATTATGGGCACGCTGTTTTTGGTCTCCCGCGTGCTCGACGCCGTCACCGACCCTCTGATGGGGCTGCTGGTAGAccgcacccgca                                                                                                           <  1:461583/140‑1 (MQ=255)                            aCGATGTTCCTGCTGGCCTATTTCTACACCGACGTCTTCGGCCTGTCGGCGGGGATTATGGGCACGCTGTTTTTGGTCTCCCGCGTGCTCGACGCCGTCACCGACCCTCTGATGGGGCTGCTGGTAGAccgcacccgcac                                                                                                          <  2:108479/140‑1 (MQ=255)                               aTGTTCCTGCTGGCCTATTTCTACACCGACGTCTTCGGCCTGTCGGCGGGGATTATGGGCACGCTGTTTTTGGTCTCCCGCGTGCTCGACGCCGTCACCGACCCTCTGATGGGGCTGCTGGTAGACCGCACCCGCAcgcg                                                                                                       >  2:428592/1‑140 (MQ=255)                                     ctgctgGCCTATTTCTACACCGACGTCTTCGGCCTGTCGGCGGGGATTATGGGCACGCTGTTTTTGGTCTCCCGCGTGCTCGACGCCGTCACCGACCCTCTGATGGGGCTGCTGGTAGACCGCACCCGCACGCGGCACgg                                                                                                 >  2:661843/1‑140 (MQ=255)                                      tgctgGCCTACTTCTACACCGACGTCCTCGGGCTGGCGGCGGGGATTATGGGCCCGTTGTTTTTGGTCTCCCGCGTGCTCGACGCCGTCACCGCCCCTCTGATGGCGCTGCTGGTAGACCGAACCCGCACGCGGCACGGc                                                                                                <  2:942723/140‑1 (MQ=255)                                                tttCTACACCGACGTCTTCGGCCTGTCGGCGGGGATTATGGGCACGCTGTTTTTGGTCTCCCGCGTGCTCGACGCCGTCACCGACCCTCTGATGGGGCTGCTGGTAGACCGCACCCGCACGCGGCACGGCCAGTTCCGcc                                                                                      <  2:6289/140‑1 (MQ=255)                                                tttCTACACCGACGTCTTCGGCCTGTCGGCGGGGATTATGGGCACGCTGTTTTTGGTCTCCCGCGTGCTCGACGCCGTCACCGACCCTCTGATGGGGCTGCTGGTAGACCGCACCCGCACGCGGCACGGCCAGTTCCGcc                                                                                      <  2:75111/140‑1 (MQ=255)                                                    tACACCGACGTCTTCGGCCTGTCGGCGGGGATTATGGGCACGCTGTTTTTGGTCTCCCGCGTGCTCGACGCCGTCACCGACCCTCTGATGGGGCTGCTGGTAGACCGCACCCGCACGCGGCACGGCCAGTTCCGCCCGtt                                                                                  <  2:1192756/140‑1 (MQ=255)                                                      cacCGACGTCTTCGGCCTGTCGGCGGGGATTATGGGCACGCTGTTTTTGGTCTCCCGCGTGCTCGACGCCGTCACCGACCCTCTGATGGGGCTGCTGGTAGACCGCACCCGCACGCGGCACGGCCAGTTCCGCCCGTTcc                                                                                >  2:137054/1‑140 (MQ=255)                                                        ccGACGTCTTCGGCCTGTCGGCGGGGATTATGGGCACGCTGTTTTTGGTCTCCCGCGTGCTCGACGCCGTCACCGACCCTCTGATGGGGCTGCTGGTAGACCGCACCCGCACGCGGCACGGCCAGTTCCGCCCGTTCctg                                                                              <  1:187163/140‑1 (MQ=255)                                                        ccGACGTCTTCGGCCTGTCGGCGGGGATTATGGGCACGCTGTTTTTGGTCTCCCGCGTGCTCGACGCCGTCACCGACCCTCTGATGGGGCTGCTGGTAGACCGCACCCGCACGCGGCACGGCCAGTTCCGCCCGTTCctg                                                                              <  1:165929/140‑1 (MQ=255)                                                        ccGACGTCTTCGGCCTGTCGGCGGGGATTATGGGCACGCTGTTTTTGGTCTCCCGCGTGCTCGACGCCGTCACCGACCCTCTGATGGGGCTGCTGGTAGACCGCACCCGCACGCGGCACGGCCAGTTCCGCCCGTTCctg                                                                              <  1:137054/140‑1 (MQ=255)                                                          gACGTCTTCGGCCTGTCGGCGGGGATTATGGGCACGCTGTTTTTGGTCTCCCGCGTGCTCGACGCCGTCACCGACCCTCTGATGGGGCTGCTGGTAGACCGCACCCGCACGCGGCACGGCCAGTTCCGCCCGTTCctgct                                                                            >  1:702819/1‑140 (MQ=255)                                                          gACGTCTTCGGCCTGTCGGCGGGGATTATGGGCACGCTGTTTTTGGTCTCCCGCGTGCTCGACGCCGTCACCGACCCTCTGATGGGGCTGCTGGTAGACCGCACCCGCACGCGGCACGGCCAGTTCCGCCCGTTCctgct                                                                            >  1:722275/1‑140 (MQ=255)                                                          gACGTCTTCGGCCTGTCGGCGGGGATTATGGGCAAGCTGTTTTTTGTCTCCCGCGCGCTCGACGCCGTCACCGCCCCTCTGATGGGGCTGCTGGTAGACCGCACCCGCACGCGGCACGGCCAGTTCCGCCCGTTCctgct                                                                            >  2:216137/1‑140 (MQ=255)                                                           aCGTCTTCGGCCTGTCGGCGGGGATTATGGGCACGCTGTTTTTGGTCTCCCGCGTGCTCGACGCCGTCACCGACCCTCTGATGGGGCTGCTGGTAGACCGCACCCGCACGCGGCACGGCCAGTTCCGCCCGTTCctgctg                                                                           >  2:173832/1‑140 (MQ=255)                                                           aCGTCTTCGGCCTGTCGGCGGGGATTATGGGCACGCTGTTTTTGGTCTCCCGCGTGCTCGACGCCGTCACCGACCCTCTGATGGGGCTGCTGGTAGACCGCACCCGCACGCGGCACGGCCAGTTCCGCCCGTTCctgctg                                                                           >  2:956448/1‑140 (MQ=255)                                                           aCGTCTTCGGCCTGTCGGCGGGGATTATGGGCACGCTGTTTTTGGTCTCCCGCGTGCTCGACGCCGTCACCGACCCTCTGATGGGGCTGCTGGTAGACCGCACCCGCACGCGGCACGGCCAGTTCCGCCCGTTCctgctg                                                                           >  2:1235295/1‑140 (MQ=255)                                                           aCGTCTTCGGCCTGTCGGCGGGGATTATGGGCACGCTGTTTTTGGTCTCCCGCGTGCTCGACGCCGTCACCGACCCTCTGATGGGGCTGCTGGTAGACCGCACCCGCACGCGGCACGGCCAGTTCCGCCCGTTCctgctg                                                                           >  2:36862/1‑140 (MQ=255)                                                           aCGTCTTCGGCCTGTCGGCGGGGATTATGGGCACGCTGTTTTTGGTCTCCCGCGTGCTCGACGCCGTCACCGACCCTCTGATGGGGCTGCTGGTAGACCGCACCCGCACGCGGCACGGCCAGTTCCGCCCGTTCctgctg                                                                           >  2:904876/1‑140 (MQ=255)                                                           aCGTCTTCGGCCTGTCGGCGGGGATTATGGGCACGCTGTTTTTGGTCTCCCGCGTGCTCGACGCCGTCACCGACCCTCTGATGGGGCTGCTGGTAGACCGCACCCGCACGCGGCACGGCCAGTTCCGCCCGTTCctgctg                                                                           >  1:827378/1‑140 (MQ=255)                                                           aCGTCTTCGGCCTGTCGGCGGGGATTATGGGCACGCTGTTTTTGGTCTCCCGCGTGCTCGACGCCGTCACCGACCCTCTGATGGGGCTGCTGGTAGACCGCACCCGCACGCGGCACGGCCAGTTCCGCCCGTTCctgctg                                                                           >  1:493855/1‑140 (MQ=255)                                                           aCGTCTTCGGCCTGTCGGCGGGGATTATGGGCACGCTGTTTTTGGTCTCCCGCGTGCTCGACGCCGTCACCGACCCTCTGATGGGGCTGCTGGTAGACCGCACCCGCACGCGGCACGGCCAGTTCCGCCCGTTCctgctg                                                                           >  1:1049713/1‑140 (MQ=255)                                                           aCGTCTTCGGCCTGTCGGCGGGGATTATGGGCACGCTGTTTTTGGTCTCCCGCGTGCTCGACGCCGTCACCGACCCTCTGATGGGGCTGCTGGTAGACCGCACCCGCACGCGGCACGGCCAGTTCCGCCCGTTCctgctg                                                                           >  1:240688/1‑140 (MQ=255)                                                           aCGTCTTCGGCCTGTCGGCGGGGATTATGGGCACGCTGTTTTTGGTCTCCCGCGTGCTCGACGCCGTCACCGACCCTCTGATGGGGCTGCTGGTAGACCCCACCCGCACGCTGCACGGCCAGTTCCGCCCGTTTctgctg                                                                           >  1:317909/1‑140 (MQ=255)                                                            cGTCTTCGGCCTGTCGGCGGGGATTATGGGCACGCTGTTTTTGGTCTCCCGCGTGCTCGACGCCGTCACCGACCCTCTGATGGGGCTGCTGGTAGACCGCACCCGCACGCGGCACGGCCAGTTCCGCCCGTTCCTGCtgt                                                                          <  1:173832/140‑1 (MQ=255)                                                              tCTTCGGCCTGTCGGC‑GGGATTATGGGCACGCTGTTTTTGGTCTCCCGCGTGCTCGACGCCGTCACCGACCCTCTGATGGGGCTGCTGGTAGACCGCACCCGCACGCGGCACGGCCAGTTCCGCCCGTTCCTGCTGTggg                                                                       >  2:15857/1‑140 (MQ=255)                                                                  cGGCCTGTCGGCGGGGATTATGGGCACGCTGTTTTTGGTCTCCCGCGTGCTCGACGCCGTCACCGACCCTCTGATGGGGCTGCTGGTAGACCGCACCCGCACGCGGCACGGCCAGTTCCGCCCGTTCCTGCTGTgggggg                                                                    <  2:1060013/140‑1 (MQ=255)                                                                       tGTCGGCGGGGATTATGGGCACGCTGTTTTTGGTCTCCCGCGTGCTCGACGCCGTCACCGACCCTCTGATGGGGCTGCTGGTAGACCGCACCCGCACGCGGCACGGCCAGTTCCGCCCGTTCCTGCTGTGGGGGGCCATc                                                               <  1:244012/140‑1 (MQ=255)                                                                        gTCGGCGGGGATTATGGGCACGCTGTTTTTGGTCTCCCGCGTGCTCGACGCCGTCACCCACACTCTGGGGGGGCTGCTAGCAGAACGCACCCGCACGCGGCACTGCCCGTACCGCCCTATCCCGCTGTGTGGGGCCATcc                                                              >  2:354415/1‑140 (MQ=255)                                                                           ggcggGGATTATGGGCACGCTGTTTTTGGTCTCCCGCGTGCTCGACGCCGTCACCGACCCTCTGATGGGGCTGCTGGTAGACCGCACCCGCACGCGGCACGGCCAGTTCCGCCCGTTCCTGCTGTGGGGGGCCATCCCGt                                                           <  1:1227146/140‑1 (MQ=255)                                                                           ggcggGGATTATGGGCACGCTGTTTTTGGTCTCCCGCGTGCTCGACGCCGTCACCGACCCTCTGATGGGGCTGCTGGTAGACCGCACCCGCACGCGGCACGGCCAGTTCCGCCCGTTCCTGCTGTGGGGGGCCATCCCGt                                                           <  1:36862/140‑1 (MQ=255)                                                                               gggATTATGGGCACGCTGTTTTTGGTCTCCCGCGTGCTCGACGCCGTCACCGACCCTCTGATGGGGCTGCTGGTAGACCGCACCCGCACGCGGCACGGCCAGTTCCGCCCGTTCCTGCTGTGGGGGGCCATCCCGTTCgg                                                       <  1:904876/140‑1 (MQ=255)                                                                                 gATTATGGGCACGCTGTTTTTGGTCTCCCGCGTGCTCGACGCCGTCACCGACCCTCTGATGGGGCTGCTGGTAGACCGCACCCGCACGCGGCACGGCCAGTTCCGCCCGTTCCTGCTGTGGGGGGCCATCCCGTTCGGCa                                                     >  1:1205913/1‑140 (MQ=255)                                                                                            cGCTGTTTTTGGTCTCCCGCGAGCTCGACGCCGTCACCGACCCTCTGATGGGGCTGCTGGTAGACCGCACCCGCACGCGGCACGGCCAGTTCCGCCCGTTCCTGCTGTGGGGGGCCATCCCGTTCGGCATCGTCTGCGTg                                          >  1:320879/1‑140 (MQ=255)                                                                                             gCTGTTTTTGGTCTCCCGCGTGCTCGACGCCGTCACCGACCCTCTGATGGGGCTGCTGGTAGACCGCACCCGCACGCGGCACGGCCAGTTCCGCCCGTTCCTGCTGTGGGGGGCCATCCCGTTCGGCATCGTCTGCGTGc                                         <  2:827378/140‑1 (MQ=255)                                                                                               tGTTTTTGGTCTCCCGCGTGCTCGACGCCGTCACCGACCCTCTGATGGGGCTGCTGGTAGACCGCACCCGCACGCGGCACGGCCAGTTCCGCCCGTTCCTGCTGTGGGGGGCCATCCCGTTCGGCATCGTCTGCGTGCTg                                       <  2:901759/140‑1 (MQ=255)                                                                                                         ctcCCGCGTGCTCGACGCCGTCACCGACCCTCTGATGGGGCTGCTGGTAGACCGCACCCGCACGCGGCACGGCCAGTTCCGCCCGTTCCTGCTGTGGGGGGCCATCCCGTTCGGCATCGTCTGCGTGCTGACCTTCTaca                             >  1:792450/1‑140 (MQ=255)                                                                                                           cccGCGTGCTCGACGCCGTCACCGACCCTCTGATGGGGCTGCTGGTAGACCGCACCCGCACGCGGCACGGCCAGTTCCGCCCGTTCCTGCTGTGGGGGGCCATCCCGTTCGGCATCGTCTGCGTGCTGACCTTCTACACg                           >  2:611967/1‑140 (MQ=255)                                                                                                           cccGCGTGCTCGACGCCGTCACCGACCCTCTGATGGGGCTGCTGGTAGACCGCACCCGCACGCGGCACGGCCAGTTCCGCCCGTTCCTGCTGTGGGGGGCCATCCCGTTCGGCATCGTCTGCGTGCTGACCTTCTACACg                           >  2:1149507/1‑140 (MQ=255)                                                                                                              gcgTGCTCGACGCCGTCACCGACCCTCTGATGGGGCTGCTGGTAGACCGCACCCGCACGCGGCACGGCCAGTTCCGCCTGTTCCTGCTGTGGGGGGCCATCCCGTTCGGCATCGTCTGCGTGCTGACCTTCTACACGCCg                        <  2:568600/140‑1 (MQ=255)                                                                                                              gcgTGCTCGACGCCGTCACCGACCCTCTGATGGGGCTGCTGGTAGACCGCACCCGCACGCGGCACGGCCAGTTCCGCCCGTTCCTGCTGTGGGGGGCCATCCCGTTCGGCATCGTCTGCGTGCTGACCTTCTACACGCCg                        <  1:1160590/140‑1 (MQ=255)                                                                                                              gcgTGCTCGACGCCGTCACCGACCCTCTGATGGGGCTGCTGGTAGACCGCACCCGCACGCGGCACGGCCAGTTCCGCCCGTTCCTGCTGTGGGGGGCCATCCCGTTCGGCATCGTCTGCGTGCTGACCTTCTACACGCCg                        <  2:702819/140‑1 (MQ=255)                                                                                                                gTGCTCGCCGCCGTCACCGACCCTCTGATGGGGCTGCTGGTAGACCGCACCCGCACGCGGCACGGCCAGTTCCGTCCGTTCCTGCTGTTGGGGGCCGACCCGTTCGGCATCGTCTGCGTGCTGACCTTCTCCACGCCGGa                      <  2:1104435/140‑1 (MQ=255)                                                                                                                gTGCTCGACGCCGTCACCGACCCTCTGATGGGGCTGCTGGTAGACCGCACCCGCACGCGGCACGGCCAGTTCCGCCCGTTCCTGCTGTGGGGGGCCATCCCGTTCGGCATCGTCTGCGTGCTGACCTTCTACACGCCGGa                      <  2:209026/140‑1 (MQ=255)                                                                                                                gTGCTCGACGCCGTCACCGACCCTCTGATGGGGCTGCTGGTAGACCGCACCCGCACGCGGCACGGCCAGTTCCGCCCGTTCCTGCTGTGGGGGGCCATCCCGTTCGGCATCGTCTGCGTGCTGACCTTCTACACGCCGGa                      <  1:354415/140‑1 (MQ=255)                                                                                                                gTGCTCGACGCCGTCACCGACCCTCTGATGGGGCTGCTGGTAGACCGCACCCGCACGCGGCACGGCCAGTTCCGCCCGTTCCTGCTGTGGGGGGCCATCCCGTTCGGCATCGTCTGCGTGCTGACCTTCTACACGCCGGa                      <  2:240688/140‑1 (MQ=255)                                                                                                                gTGCTCGACGCCGTCACCGACCCTCTGATGGGGCTGCTGGTAGACCGCACCCGCACGCGGCACGGCCAGTTCCGCCCGTTCCTGCTGTGGGGGGCCATCCCGTTCGGCATCGTCTGCGTGCTGACCTTCTACACGCCGGa                      <  1:956448/140‑1 (MQ=255)                                                                                                                gTGCTCGACGCCGTCACCGACCCTCTGATGGGGCTGCTGGTAGACCGCACCCGCACGCGGCACGGCCAGTTCCGCCCGTTCCTGCTGTGGGGGGCCATCCCGTTCGGCATCGTCTGCGTGCTGACCTTCTACACGCCGGa                      <  2:1180925/140‑1 (MQ=255)                                                                                                                gTGCTCGACGCCGTCACCGACCCTCTGATGGGGCTGCTGGTAGACCGCACCCGCACGCGGCACGGCCAGTTCCGCCCGTTCCTGCTGTGGGGGGCCATCCCGTTCGGCATCGTCTGCGTGCTGACCTTCTACACGCCGGa                      <  2:908883/140‑1 (MQ=255)                                                                                                                gTGCTCGACGCCGTCACCGACCCTCTGATGGGGCTGCTGGTAGACCGCACCCGCACGCGGCACGGCCAGTTCCGCCCGTTCCTGCTGTGGGGGGCCATCCCGTTCGGCATCGTCTGCGTGCTGACCTTCTACACGCCGGa                      <  1:560277/140‑1 (MQ=255)                                                                                                                gTGCTCGACGCCGTCACCGACCCTCTGATGGGGCTGCTGGTAGACCGCACCCGCACGCGGCACGGCCAGTTCCGCCCGTTCCTGCTGTGGGGGGCCATCCCGTTCGGCATCGTCTGCGTGCTGACCTTCTACACGCCGGa                      <  1:657385/140‑1 (MQ=255)                                                                                                                gTGCTCGACGCCGTCACCGACCCTCTGATGGGGCTGCTGGTAGACCGCACCCGCACGCGGCACGGCCAGTTCCGCCCGTTCCTGCTGTGGGGGGCCATCCCGTTCGGCATCGTCTGCGTGCTGACCTTCTACACGCCGGa                      <  1:644285/140‑1 (MQ=255)                                                                                                                gTGCTCGACGCCGTCACCGACCCTCTGATGGGGCTGCTGGTAGACCGCACCCGCACGCGGCACGGCCAGTTCCGCCCGTTCCTGCTGTGGGGGGCCATCCCGTTCGGCATCGTCTGCGTGCTGACCTTCTACACGCCGGa                      <  2:1205913/140‑1 (MQ=255)                                                                                                                      gACGCCGTCACCGACCCTCTGATGGGGCTGCTGGTAGACCGCACCCGCACGCGGCACGGCCAGTTCCGCCCGTTCCTGCTGTGGGGGGCCATCCCGTTCGGCATCGTCTGCGTGCTGACCTTCTACACGCCGGa                      <  2:1009202/134‑1 (MQ=255)                                                                                                                      gACGCCGTCACCGACCCTCTGATGGGGCTGCTGGTAGACCGCACCCGCACGCGGCACGGCCAGTTCCGCCCGTTCCTGCTGTGGGGGGCCATCCCGTTCGGCATCGTCTGCGTGCTGACCTTCTACACGCCGGa                      >  1:1009202/1‑134 (MQ=255)                                                                                                                      gACGCCGTCACCGACCCTCTGATGGGGCTGCTGGTAGACCGCACCCGCACGCGGCACGGCCAGTTCCGCCCGTTCCTGCTGTGGGGGGCCATCCCGTTCGGCATCGTCTGCGTGCTGACCTTCTACACGCCGGACTtctc                >  2:1202704/1‑140 (MQ=255)                                                                                                                          ccGTCACCGACCCTCTGATGGGGCTGCTGGTAGACCGCACCCGCACGCGGCACGGCCAGTTCCGCCCGTTCCTGCTGTGGGGGGCCATCCCGTTCGGCATCGTCTGCGTGCTGACCTTCTACACGCCGGACTTCTCCGca            <  2:546958/140‑1 (MQ=255)                                                                                                                          ccGTCACCGACCCTCTGATGGGGCTGCTGGTAGACCGCACCCGCACGCGGCACGGCCAGTTCCGCCCGTTCCTGCTGTGGGGGGCCATCCCGTTCGGCATCGTCTGCGTGCTGACCTTCTACACGCCGGACTTCTCCGca            <  2:1049713/140‑1 (MQ=255)                                                                                                                          ccGTCACCGACCCTCTGATGGGGCTGCTGGTAGACCGCACCCGCACGCGGCACGGCCAGTTCCGCCCGTTCCTGCTGTGGGGGGCCATCCCGTTCGGCATCGTCTGCGTGCTGACCTTCTACACGCCGGACTTCTCCGca            <  2:912766/140‑1 (MQ=255)                                                                                                                          ccGTCACCGACCCTCTGATGGGGCTGCTGGTAGACCGCACCCGCACGCGGCACGGCCAGTTCCGCCCGTTCCTGCTGTGGGGGGCCATCCCGTTCGGCATCGTCTGCGTGCTGACCTTCTACACGCCGGACTTCTCCGca            <  1:1014679/140‑1 (MQ=255)                                                                                                                           cGTCACCGACCCTCTGATGGGGCTGCTGGTAGACCGCGCCCGCACGCGGCACGGCCAGTTCCGCCCGTTCCTGCTGTGGGGGGCCATCCCGTTCGGCATCGTCTGCGTGCTGACCTTCTACACGCCGGACTTCTCCGcac           <  2:320879/140‑1 (MQ=255)                                                                                                                             tCACCGACCCTCTGATGGGGCTGCTGGTAGACCGCACCCGCACGCGGCACGGCCAGTTCCGCCCGTTCCTGCTGTGGGGGGCCATCCCGTTCGGCATCGTCTGCGTGCTGACCTTCTACACGCCGGACTTCTCCGCACAg         >  1:480736/1‑140 (MQ=255)                                                                                                                              cACCGACCCTCTGATGGGGCTGCTGGTAGACCGCACCCGCACGCGGCACGGCCAGTTCCGCCCGTTCCTGCTGTGGGGGGCCATCCCGTTCGGCATCGTCTGCGTGCTGACCTTCTACACGCCGGACTTCTCCGCACAgg        <  1:1235295/140‑1 (MQ=255)                                                                                                                               aCCGACCCTCTGATGGGGCTGCTGGTAGACCGCACCCGCACGCGGCACGGCCAGTTCCGCCCGTTCCTGCTGTGGGGGGCCATCCCGTTCGGCATCGTCTGCGTGCTGACCTTCTACACGCCGGACTTCTCCGCACAggg       <  2:480736/140‑1 (MQ=255)                                                                                                                                ccGACCCTCTGATGGGGCTGCTGGTAGACCGCACCCGCACGCGGCACGGCCAGTTCCGCCCGTTCCTGCTGTGGGGGGCCATCCCGTTCGGCATCGTCTGCGTGCTGACCTTCTACACGCCGGACTTCTCCGCACAGGGc      <  1:596458/140‑1 (MQ=255)                                                                                                                                   aCCCTCTGATGGGGCTGCTGGTAGACCGCACCCGCACGCGGCACGGCCAGTTCCGCCCGTTCCTGCTGTGGGGGGCCATCCCGTTCGGCATCGTCTGCGTGCTGACCTTCTACACGCCGGACTTCTCCGCACAGGGCAAg   <  2:493855/140‑1 (MQ=255)                                                                                                                                    cccTCTGATGGGGCTGCTGGTAGACCGCACCCGCACGCGGCACGGCCAGTTCCGCCCGTTCCTGCTGTGGGGGGCCATCCCGTTCGGCATCGTCTGCGTGCTGACCTTCTACACGCCGGACTTCTCCGCACAGGGCAAGa  <  1:360060/140‑1 (MQ=255)                                                                                                                                       |                                                                                                                                         ACCGCCTGCGGCTTCGTCTGGCAGGCCACGATGTTCCTGCTGGCCTATTTCTACACCGACGTCTTCGGCCTGTCGGCGGGGATTATGGGCACGCTGTTTTTGGTCTCCCGCGTGCTCGACGCCGTCACCGACCCGCTGATGGGGCTGCTGGTAGACCGCACCCGCACGCGGCACGGCCAGTTCCGCCCGTTCCTGCTGTGGGGGGCCATCCCGTTCGGCATCGTCTGCGTGCTGACCTTCTACACGCCGGACTTCTCCGCACAGGGCAAGA  >  NC_000913/285443‑285713

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 18 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑