Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	4,332,541	G→T	D244E (GAC→GAA)	basS ←	sensory histidine kinase BasS

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	4,332,541	0	G	T	100.0%	330.2 / NA	93	D244E (GAC→GAA)	basS	sensory histidine kinase BasS
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base T (40/53);  total (40/53)

CTGCCATGACCGCTCCGTCATCTTCTTGCAGCTTAATCATAATGTTGCTGCCTTGCGGGCTGTAACGATGGGCGTTTTCTACCAGGTTTCGCAATAACATCCGCAGCAGGGTTGCATCACCCTGAACGGTGATGTCGGCGGCGCTCTCTGGCAATAGCAGGGTTTGCTGTCGCTGGTCGAGCATGGTACTGAGTTCGTCATACGAGGGGAGAATGACATCTTCCAGCAGTTTTACATGTTGATAATTACCGGAAGAAAATGACTGTCCGG  >  NC_000913/4332408‑4332677                                                                                                                                      |                                                                                                                                         cTGCCATGACCGCTCCGTCATCTTCTTGCAGCTTAATCATAATGTTGCTGCCTTGCGGGCTGTAACGATGGGCGTTTTCTACCAGGTTTCGCAATAACATCCGCAGCAGGGTTGCATCACCCTGAACGGTGATTTcggcg                                                                                                                                    >  1:707407/1‑140 (MQ=255)  tGCCATGACCGCTCCGTCATCTTCTTGCAGCTTAATCATAATGTTGCTGCCTTGCGGGCTGTAACGATGGGCGTTTTCTACCAGGTTTCGCAATAACATCCGCAGCAGGGTTGCATCACCCTGAACGGTGATTTcggcgg                                                                                                                                   <  1:1437078/140‑1 (MQ=255)  tGCCATGACCGCTCCGTCATCTTCTTGCAGCTTAATCATAATGTTGCTGCCTTGCGGGCTGTAACGATGGGCGTTTTCTACCAGGTTTCGCAATAACATCCGCAGCAGGGTTGCATCACCCTGAACGGTGATTTcggcgg                                                                                                                                   <  2:638388/140‑1 (MQ=255)    ccATGACCGCTCCGTCATCTTCTTGCAGCTTAATCATAATGTTGCTGCCTTGCGGGCTGTAACGATGGGCGTTTTCTACCAGGTTTCGCAATAACATCCGCAGCAGGGTTGCATCACCCTGAACGGTGATTTCGgcggcg                                                                                                                                 >  2:1060346/1‑140 (MQ=255)    ccATGACCGCTCCGTCATCTTCTTGCAGCTTAATCATAATGTTGCTGCCTTGCGGGCTGTAACGATGGGCGTTTTCTACCAGGTTTCGCAATAACATCCGCAGCAGGGTTGCATCACCCTGAACGGTGATTTCGgcggcg                                                                                                                                 <  2:87739/140‑1 (MQ=255)    ccATGACCGCTCCGTCATCTTCTTGCAGCTTAATCATAATGTTGCTGCCTTGCGGGCTGTAACGATGGGCGTTTTCTACCAGGTTTCGCAATAACATCCGCAGCAGGGTTGCATCACCCTGAACGGTGATTTCGgcggcg                                                                                                                                 >  2:665448/1‑140 (MQ=255)      aTGACCGCTCCGTCATCTTCTTGCAGCTTAATCATAATGTTGCTGCCTTGCGGGCTGTAACGATGGGCGTTTTCTACCAGGTTTCGCAATAACATCCGCAGCAGGGTTGCATCACCCTGAACGGTGATTTCGGCGGCGct                                                                                                                               >  2:731675/1‑140 (MQ=255)        gACCGCTCCGTCATCTTCTTGCAGCTTAATCATAATGTTGCTGCCTTGCGGGCTGTAACGATGGGCGTTTTCTACCAGGTTTCGCAATAACATCCGCAGCAGGGTTGCATCACCCTGAACGGTGATTTCGGCGGCGctct                                                                                                                             <  1:1060346/140‑1 (MQ=255)         aCCGCTCCGTCATCTTCTTGCAGCTTAATCATAATGTTGCTGCCTTGCGGGCTGTAACGATGGGCGTTTTCTACCAGGTTTCGCAATAACATCCGCAGCAGGGTTGCATCACCCTGAACGGTGATTTCGGCGGCGctctc                                                                                                                            >  2:907684/1‑140 (MQ=255)            gCTCCGTCATCTTCTTGCAGCTTAATCATAATGTTGCTGCCTTGCGGGCTGTAACGATGGGCGTTTTCTACCAGGTTTCGCAATAACATCCGCAGCAGGGTTGCATCACCCTGAACGGTGATTTCGGCGGCGCTCTCTgg                                                                                                                         <  2:98278/140‑1 (MQ=255)             cTCCGTTATCTTCTTGCAGCTTAATCATAATGTTGCTGCCTTGCGGGCTGTAACGATGGGCGTTTTCTACCAGGTTTCGCAATAACATCCGCAGCAGGGTTGCATCACCCTGAACGGTGATTTCGGCGGCGCTCTCTGGc                                                                                                                        >  1:143295/1‑140 (MQ=255)              tCCGTCATCTTCTTGCAGCTTAATCATAATGTTGCTGCCTTGCGGGCTGTAACGATGGGCGTTTTCTACCAGGTTTCGCAATAACATCCGCAGCAGGGTTGCATCACCCTGAACGGTGATTTCGGCGGCGCTCTCTGGCa                                                                                                                       <  1:571907/140‑1 (MQ=255)              tCCGTCATCTTCTTGCAGCTTAATCATAATGTTGCTGCCTTGCGGGCTGTAACGATGGGCGTTTTCTACCAGGTTTCGCAATAACATCCGCAGCAGGGTTGCATCACCCTGAACGGTGATTTCGGCGGCGCTCTCTGGCa                                                                                                                       >  1:229901/1‑140 (MQ=255)              tCCGTCATCTTCTTGCAGCTTAATCATAATGTTGCTGCCTTGCGGGCTGTAACGATGGGCGTTTTCTACCAGGTTTCGCAATAACATCCGCAGCAGGGTTGCATCACCCTGAACGGAGATTTCGGCGGCGCTCTCTGGCa                                                                                                                       >  1:1083299/1‑140 (MQ=255)                cGTCATCTTCTTGCAGCTTAATCATAATGTTGCTGCCTTGCGGGCTGTAACGATGGGCGTTTTCTACCAGGTTTCGCAATAACATCCGCAGCAGGGTTGCATCACCCTGAACGGTGATTTCGGCGGCGCTCTCTGGCAAt                                                                                                                     >  2:1052015/1‑140 (MQ=255)                  tCATCTTCTTGCAGCTTAATCATAATGTTGCTGCCTTGCGGGCTGTAACGATGGGCGTTTTCTACCAGGTTTCGCAATAACATCCGCAGCAGGGTTGCATCACCCTGAACGGTGATTTCGGCGGCGCTCTCTGGCAATAg                                                                                                                   >  2:1421363/1‑140 (MQ=255)                                cTTAATCATAATGTTGCTGCCTTGCGGGCTGTAACGATGGGCGTTTTCTACCAGGTTTCGCAATAACATCCGCAGCAGGGTTGCATCACCCTGAACGGTGATTTCGGCGGCGCTCTCTGGCAATAGCAGGGTTTGCt                                                                                                        >  2:797643/1‑137 (MQ=255)                                cTTAATCATAATGTTGCTGCCTTGCGGGCTGTAACGATGGGCGTTTTCTACCAGGTTTCGCAATAACATCCGCAGCAGGGTTGCATCACCCTGAACGGTGATTTCGGCGGCGCTCTCTGGCAATAGCAGGGTTTGCt                                                                                                        <  1:797643/137‑1 (MQ=255)                                 ttAATCATAATGTTGCTGCCTTGCGGGCTGTAACGATGGGCGTTTTCTACCAGGTTTCGCAATAACATCCGCAGCAGGGTTGCATCACCCTGAACGGTGATTTCGGCGGCGCTCTCTGGCaa                                                                                                                      <  2:1417039/122‑1 (MQ=255)                                 ttAATCATAATGTTGCTGCCTTGCGGGCTGTAACGATGGGCGTTTTCTACCAGGTTTCGCAATAACATCCGCAGCAGGGTTGCATCACCCTGAACGGTGATTTCGGCGGCGCTCTCTGGCaa                                                                                                                      >  1:1417039/1‑122 (MQ=255)                                 ttAATCATAATGTTGCTGCCTTGCGGGCTGTAACGATGGGCGTTTTCTACCAGGTTTCGCAATAACATCCGCAGCAGGGTTGCATCACCCTGAACGGTGATTTCGGCGGCGCTCTCTGGCAATAGCAGGGTTTGCTGTCg                                                                                                    <  1:229030/140‑1 (MQ=255)                                 ttAATCATAATGTTGCTGCCTTGCGGGCTGTAACGATGGGCGTTTTCTACCAGGTTTCGCAATAACATCCGCAGCAGGGTTGCATCACCCTGAACGGTGATTTCGGCGGCGCTCTCTGGCAATAGCAGGGTTTGCTGTCg                                                                                                    >  1:225556/1‑140 (MQ=255)                                     tCATAATGTTGCTGCCTTGCGGGCTGTAACGATGGGCGTTTTCTACCAGGTTTCGCAATAACATCCGCAGCAGGGTTGCATCACCCTGAACGGTGATTTCGGCGGCGCTCTCTGGCAATAGCAGGGTTTGCTGTCGCTgg                                                                                                <  1:23015/140‑1 (MQ=255)                                     tCATAATGTTGCTGCCTTGCGGGCTGTAACGATGGGCGTTTTCTACCAGGTTTCGCAATAACATCCGCAGCAGGGTTGCATCACCCTGAACGGTGATTTCGGCGGCGCTCCCTGGCAATAGCAGGGTTTGCTGTCGCTgg                                                                                                <  1:1155522/140‑1 (MQ=255)                                            gTTGCTGCCTTGCGGGCTGTAACGATGGGCGTTTTCTACCAGGTTTCGCAATAACATCCGCAGCAGGGTTGCATCACCCTGAACGGTGATTTCGGCGGCGCTCTCTGGCAATAGCAGGGTTTGCTGTCGCTGGTCGAGCa                                                                                         <  1:731675/140‑1 (MQ=255)                                            gTTGCTGCCTTGCGGGCTGTAACGATGGGCGTTTTCTACCAGGTTTCGCAATAACATCCGCAGCAGGGTTGCATCACCCTGAACGGTGATTTCGGCGGCGCTCTCTGGCAATAGCAGGGTTTGCTGTCGCTGGTCGAGCa                                                                                         <  2:591515/140‑1 (MQ=255)                                            gTTGCTGCCTTGCGGGCTGTAACGATGGGCGTTTTCTACCAGGTTTCGCAATAACATCCGCAGCAGGGTTGCATCACCCTGAACGGTGATTTCGGCGGCGCTCTCTGGCAATAGCAGGGTTTGCTGTCGCTGGTCGAGCa                                                                                         <  2:650195/140‑1 (MQ=255)                                              tgctgcCTTGCGGGCTGTAACGATGGGCGTTTTCTCCCAGGATTCGCAATAAAATCCGCAGAAGGGTTGCATCACCCTGAACGGTGATTTCGGCGGCGCTCTCTGGCAATAGCAGGGTTTGCTGTCGCTGGTCGAGCATg                                                                                       >  2:912139/1‑140 (MQ=255)                                              tgctgcCTTGCGGGCTGTAACGATGGGCGTTTTCTACCAGGTTTCGCAATAACATCCGCAGCAGGGTTGCATCACCCTGAACGGTGATTTCGGCGGCGCTCTCTGGCAATAGCAGGGTTTGCTGTCGCTGGTCGAGCATg                                                                                       >  1:153780/1‑140 (MQ=255)                                               gctgcCTTGCGGGCTGTAACGATGGGCGTTTTCTACCAGGTTTCGCAATAACATCCGCAGCAGGGTTGCATCACCCTGAACGGTGATTTCGGCGGCGCTCTCTGGCAATAGCAGGGTTTGCTGTCGCTGGTCGa                                                                                            <  1:1327142/134‑1 (MQ=255)                                               gctgcCTTGCGGGCTGTAACGATGGGCGTTTTCTACCAGGTTTCGCAATAACATCCGCAGCAGGGTTGCATCACCCTGAACGGTGATTTCGGCGGCGCTCTCTGGCAATAGCAGGGTTTGCTGTCGCTGGTCGa                                                                                            >  2:1327142/1‑134 (MQ=255)                                               gctgcCTTGCGGGCTGTAACGATGGGCGTTTTCTACCAGGTTTCGCAATAACATCCGCAGCAGGGTTGCATCACCCTGAACGGTGATTTCGGCGGCGCTCTCTGGCAATAGCAGGGTTTGCTGTCGCTGGTCGAGCATgg                                                                                      >  2:782890/1‑140 (MQ=255)                                               gctgcCTTGCGGGCTGTAACGATGGGCGTTTTCTACCAGGTTTCGCAATAACATCCGCAGCAGGGTTGCATCACCCTGAACGGTGATTTCGGCGGCGCTCTCTGGCAATAGCAGGGTTTGCTGTCGCTGGTCGAGCATgg                                                                                      >  1:15669/1‑140 (MQ=255)                                               gctgcCTTGCGGGCTGTAACGATGGGCGTTTTCTACCAGGTTTCGCAATAACATCCGCAGCAGGGTTGCATCACCCTGAACGGTGATTTCGGCGGCGCTCTCTGGCAATAGCAGGGTTTGCTGTCGCTGGTCGAGCATgg                                                                                      >  2:272325/1‑140 (MQ=255)                                                ctgcCTTGTGGGCTGTAACGATGGGCGTTTTCTACCAGGTTTCGCAATAACATCCGCAGCAGGGTTGCATCACCCTGAACGGTGATTTCGGCGGCGCTCTCTGGCAATAGCAGGGTTTGCTGTCGCTGGTCGAGCATGGt                                                                                     >  1:896141/1‑140 (MQ=255)                                                ctgcCTTGCGGGCTGTAACGATGGGCGTTTTCTACCAGGTTTCGCAATAACATCCGCAGCAGGGTTGCATCACCCTGAACGGTGATTTCGGCGGCGCTCTCTGGCAATAGCAGGGTTTGCTGTCGCTgg                                                                                                <  1:1440318/129‑1 (MQ=255)                                                ctgcCTTGCGGGCTGTAACGATGGGCGTTTTCTACCAGGTTTCGCAATAACATCCGCAGCAGGGTTGCATCACCCTGAACGGTGATTTCGGCGGCGCTCTCTGGCAATAGCAGGGTTTGCTGTCGCTgg                                                                                                >  2:1440318/1‑129 (MQ=255)                                                ctgcCTTGCGGGCTGTAACGATGGGCGTTTTCTACCAGGTTTCGCAATAACATCCGCAGCAGGGTTGCATCACCCTGAACGGTGATTTCGGCGGCGCTCTCTGGCAATAGCAGGGTTTGCTGTCGCTGGTCGAGCATGGt                                                                                     >  2:847717/1‑140 (MQ=255)                                                ctgcCTTGCGGGCTGTAACGATGGGCGTTTTCTACCAGGTTTCGCAATAACATCCGCAGCAGGGTTGCATCACCCTGAACGGTGATTTCGGCGGCGCTCTCTGGCAATAGCAGGGTTTGCTGTCGCTGGTCGAGCATGGt                                                                                     >  1:789181/1‑140 (MQ=255)                                                 tgcCTTGCGGGCTGTAACGATGGGCGTTTTCTACCAGGTTTCGCAATAACATCCGCAGCAGGGTTGCATCACCCTGAACGGTGATTTCGGCGGCGCTCTCTGGCAATAGCAGGGTTTGCTGTCGCTGGTCGAGCATGGTa                                                                                    <  1:684136/140‑1 (MQ=255)                                                   ccTTGCGGGCTGTAACGATGGGCGTTTTCTACCAGGTTTCGCAATAACATCCGCAGCAGGGTTGCATCACCCTGAACGGTGATTTCGGCGGCGCTCTCTGGCAATAGCAGGGTTTGCTGTCGCTGGTCGAGCATGGTAc                                                                                   <  1:1115200/139‑1 (MQ=255)                                                   ccTTGCGGGCTGTAACGATGGGCGTTTTCTACCAGGTTTCGCAATAACATCCGCAGCAGGGTTGCATCACCCTGAACGGTGATTTCGGCGGCGCTCTCTGGCAATAGCAGGGTTTGCTGTCGCTGGTCGAGCATGGTAc                                                                                   >  2:1115200/1‑139 (MQ=255)                                                     ttGCGGGCTGTAACGATGGGCGTTTTCTACCAGGTTTCGCAATAACATCCGCAGCAGGGTTGCATCACCCTGAACGGTGATTTCGGCGGCGCTCTCTGGCAATAGCAGGGTTTGCTGTCGCTGGTCGAGCATGGTACTGa                                                                                >  2:1110648/1‑140 (MQ=255)                                                      tGCGGGCTGTAACGATGGGCGTTTTCTACCAGGTTTCGCAATAACATCCGCAGCAGGGTTGCATCACCCTGAACGGTGATTTCGGCGGCGCTCTCTGGCAATAGCAGGGTTTGCTGTCGCTGGTCGAGCATGGTACTGAg                                                                               <  1:665448/140‑1 (MQ=255)                                                      tGCGGGCTGTAACGATGGGCGTTTTCTACCAGGTTTCGCAATAACATCCGCAGCAGGGTTGCATCACCCTGAACGGTGATTTCGGCGGCGCTCTCTGGCAATAGCAGGGTTTGCTGTCGCTGGTCGAGCATGGTACTGAg                                                                               <  2:707407/140‑1 (MQ=255)                                                       gCGGGCTGTAACGATGGGCGTTTTCTACCAGGTTTCGCAATAACATCCGCAGCAGGGTTGCATCACCCTGAACGGTGATTTCGGCGGCGCTCTCTGGCAATAGCAGGGTTTGCTGTCGCTGGTc                                                                                              >  1:645794/1‑124 (MQ=255)                                                       gCGGGCTGTAACGATGGGCGTTTTCTACCAGGTTTCGCAATAACATCCGCAGCAGGGTTGCATCACCCTGAACGGTGATTTCGGCGGCGCTCTCTGGCAATAGCAGGGTTTGCTGTCGCTGGTc                                                                                              <  2:645794/124‑1 (MQ=255)                                                       gCGGGCTGTAACGATGGGCGTTTTCTACCAGGTTTCGCAATAACATCCGCAGCAGGGTTGCATCACCCTGAACGGTGATTTCGGCGGCGCTCTCTGGCAATAGCAGGGTTTGCTGTCGCTGGTCGAGCATGGTACTGAGt                                                                              >  2:336569/1‑140 (MQ=255)                                                           gCTGTAACGATGGGCGTTTTCTACCAGGTTTCGCAATAACATCCGCAGCAGGGTTGCATCACCCTGAACGGTGATTTCGGCGGCGCTCTCTGGCAATAGCAGGGTTTGCTGTCGCTGGTCGAGCATGGTACTGAGTTCGt                                                                          <  1:272325/140‑1 (MQ=255)                                                              gTAACGATGGGCGTTTTCTACCAGGTTTCGCAATAACATCCGCAGCAGGGTTGCATCACCCTGAACGGTGATTTCGGCGGCGCTCTCTGGCAATAGCAGGGTTTGCTGTCGCTGGTCGAGCATGGTACTGAGTTCGTCAt                                                                       >  2:1382893/1‑140 (MQ=255)                                                                aaCGATGGGCGTTTTCTACCAGGTTTCGCAATAACATCCGCAGCAGGGTTGCATCACCCTGAACGGTGATTTCGGCGGCGCTCTCTGGCAATAGCAGGGTTTGCTGTCGCTGGTCGAGCATGGTACTGAGTTCGTCATAc                                                                     <  1:1080501/140‑1 (MQ=255)                                                                 aCGATGGGCGTTTTCTACCAGGTTTCGCAATAACATCCGCAGCAGGGTTGCATCACCCTGAACGGTGATTTCGGCGGCGCTCTCTGGCAATAGCAGGGTTTGCTGTCGCTGGTCGAGCATGGTACTGAGTTCGTCATACg                                                                    <  1:1289027/140‑1 (MQ=255)                                                                  cGATGGGCGTTTTCTACCAGGTTTCGCAATAACATCCGCAGCAGGGTTGCATCACCCTGAACGGTGATTTCGGCGGCGCTCTCTGGCAATAGCAGGGTTTGCTGTCGCTGGTCGAGCATGGTACTGAGTTCGTCATACGa                                                                   >  2:76039/1‑140 (MQ=255)                                                                   gATGGGCGTTTTCTACCAGGTTTCGCAATAACATCCGCAGCAGGGTTGCATCACCCTGAACGGTGATTTCGGCGGCGCTCTCTGGCAATAGCAGGGTTTGCTGTCGCTGGTCGAGCATGGTACTGAGTTCGTCATACGAg                                                                  <  1:1204841/140‑1 (MQ=255)                                                                   gATGGGCGTTTTCTACCAGGTTTCGCAATAACATCCGCAGCAGGGTTGCATCACCCTGAACGGTGATTTCGGCGGCGCTCTCTGGCAATAGCAGGGTTTGCTGTCGCTGGTCGAGCATGGTACTGAGTTCGTCATACGAg                                                                  <  1:1110648/140‑1 (MQ=255)                                                                   gATGGGCGTTTTCTACCAGGTTTCGCAATAACATCCGCAGCAGGGTTGCATCACCCTGAACGGTGATTTCGGCGGCGCTCTCTGGCAATAGCAGGGTTTGCTGTCGCTGGTCGAGCATGGTACTGAGTTCGTCATACGAg                                                                  <  1:1052015/140‑1 (MQ=255)                                                                   gATGGGCGTTTTCTACCAGGTTTCGCAATAACATCCGCAGCAGGGTTGCATCACCCTGAACGGTGATTTCGGCGGCGCTCTCTGGCAATAGCAGGGTTTGCTGTCGCTGGTCGAGCATGGTACTGAGTTCGTCATACGAg                                                                  <  1:860732/140‑1 (MQ=255)                                                                     tGGGCGTTTTCTACCAGGTTTCGCAATAACATCCGCAGCAGGGTTGCATCACCCTGAACGGTGATTTCGGCGGCGCTCTCTGGCAATAGCAGGGTTTGCTGTCGCTGGTCGAGCATGGTACTGAGTTCGTCATACGAggg                                                                >  1:894765/1‑140 (MQ=255)                                                                         cGTTTTCTACCAGGTTTCGCAATAACATCCGCAGCAGGGTTGCATCACCCTGAACGGTGATTTCGGCGGCGCTCTCTGGCAATAGCAGGGTTTGCTGTCGCTGGTCGAGCATGGTACTGAGTTCGTCATACGAGGGgaga                                                            >  2:632523/1‑140 (MQ=255)                                                                         cGTTTTCTACCAGGTTTCGCAATAACATCCGCAGCAGGGTTGCATCACCCTGAACGGTGATTTCGGCGGCGCTCTCTGGCAATAGCAGGGTTTGCTGTCGCTGGTCGAGCATGGTACTGAGTTCGTCATACGAGGGgaga                                                            <  1:76039/140‑1 (MQ=255)                                                                                      gTTTCGCAATAACATCCGCAGCAGGGTTGCATCACCCTGAACGGTGATTTCGGCGGCGCTCTCTGGCAATAGCAGGGTTTGCTGTCGCTGGTCGAGCATGGTACTGAGTTCGTCATACGAGGGGAGAATGACATCTTCca                                               <  2:225556/140‑1 (MQ=255)                                                                                       tttCGCAATAACATCCGCAGCAGGGTTGCATCACCCTGAACGGTGATTTCGGCGGCGCTCTCTGGCAATAGCAGGGTTTGCTGTCGCTGGTCGAGCATGGTACTGAGTTCGTCATACGAGGGGAGAATGACATCTTCcag                                              <  1:632523/140‑1 (MQ=255)                                                                                       tttCGCAATAACATCCGCAGCAGGGTTGCATCACCCTGAACGGTGATTTCGGCGGCGCTCTCTGGCAATAGCAGGGTTTGCTGTCGCTGGTCGAGCATGGTACTGAGTTCGTCATACGAGGGGAGAATGACATCTTCcag                                              <  1:1118046/140‑1 (MQ=255)                                                                                       tttCGCAATAACATCCGCAGCAGGGTTGCATCACCCTGAACGGTGATTTCGGCGGCGCTCTCTGGCAATAGCAGGGTTTGCTGTCGCTGGTCGAGCATGGTACTGAGTTCGTCATACGAGGGGAGAATGACATCTTCcag                                              >  2:1215473/1‑140 (MQ=255)                                                                                        ttCGCAATAACATCCGCAGCAGGGTTGCATCACCCTGAACGGTGATTTCGGCGGCGCTCTCTGGCAATAGCAGGGTTTGCTGTCGCTGGTCGAGCATGGTACTGAGTTCGTCATACGAGGGGAGAATGACATCTTCcagc                                             <  1:782890/140‑1 (MQ=255)                                                                                         tCGCAATAACATCCGCAGCAGGGTTGCATCACCCTGAACGGTGATTTCGGCGGCGCTCTCTGGCAATAGCAGGGTTTGCTGTCGCTGGTCGAGCATGGTACTGAGTTCGTCATACGAGGGGAGAATGACATCTTCcagca                                            <  2:143295/140‑1 (MQ=255)                                                                                         tCGCAATAACATCCGCAGCAGGGTTGCATCACCCTGAACGGTGATTTCGGCGGCGCTCTCTGGCAATAGCAGGGTTTGCTGTCGCTGGTCGAGCATGGTACTGAGTTCGTCATACGAGGGGAGAATGACATCTTCcagca                                            <  1:717050/140‑1 (MQ=255)                                                                                             aaTAACATCCGCAGCAGGGTTGCATCACCCTGAACGGTGATTTCGGCGGCGCTCTCTGGCAATAGCAGGGTTTGCTGTCGCTGGTCGAGCATGGTACTGAGTTCGTCATACGAGGGGAGAATGACATCTTCCAGCAGttt                                        <  2:894765/140‑1 (MQ=255)                                                                                              aTAACATCCGCAGCAGGGTTGCATCACCCTGAACGGTGATTTCGGCGGCGCTCTCTGGCAATAGCAGGGTTTGCTGTCGCTGGTCGAGCATGGTACTGAGTTCGTCATACGAGGGGAGAATGACATCTTCCAGCAGtttt                                       <  2:819559/140‑1 (MQ=255)                                                                                                aaCATCCGCAGCAGGGTTGCATCACCCTGAACGGTGATTTCGGCGGCGCTCTCTGGCAATAGCAGGGTTTGCTGTCGCTGGTCGAGCATGGTACTGAGTTCGTCATACGAGGGGAGAATGACATCTTCCAGCAGTTTTAc                                     <  2:16084/140‑1 (MQ=255)                                                                                                aaCATCCGCAGCAGGGTTGCATCACCCTGAACGGTGATTTCGGCGGCGCTCTCTGGCAATAGCAGGGTTTGCTGTCGCTGGTCGAGCATGGTACTGAGTTCGTCATACGAGGGGAGAATGACATCTTCCAGCAGTTTTAc                                     <  1:1215473/140‑1 (MQ=255)                                                                                                   aTCCGCAGCAGGGTTGCATCACCCTGAACGGTGATTTCGGCGGCGCTCTCTGGCAATAGCAGGGTTTGCTGTCGCTGGTCGAGCATGGTACTGAGTTCGTCATACGAGGGGAGAATGACATCTTCCAGCAGTTTTACATg                                  <  2:789181/140‑1 (MQ=255)                                                                                                   aTCCGCAGCAGGGTTGCATCACCCTGAACGGTGATTTCGGCGGCGCTCTCTGGCAATAGCAGGGTTTGCTGTCGCTGGTCGAGCATGGTACTGAGTTCGTCATACGAGGGGAGAATGACATCTTCCAGCAGTTTTACATg                                  <  1:610263/140‑1 (MQ=255)                                                                                                       gcagcaGGGTTGCATCACCCTGAACGGTGATTTCGGCGGCGCTCTCTGGCAATAGCAGGGTTTGCTGTCGCTGGTCGAGCATGGTACTGAGTTCGTCATACGAGGGGAGAATGACATCTTCCAGCAGTTTTACATGTTGa                              >  2:1454005/1‑140 (MQ=255)                                                                                                         agcagGGTTGCATCACCCTGAACGGTGATTTCGGCGGCGCTCTCTGGCAATAGCAGGGTTTGCTGTCGCTGGTCGAGCATGGTACTGAGTTCGTCATACGAGGGGAGAATGACATCTTCCAGCAGTTTTACATGTTGATa                            >  1:1112637/1‑140 (MQ=255)                                                                                                         agcagGGTTGCATCACCCTGAACGGTGATTTCGGCGGCGCTCTCTGGCAATAGCAGGGTTTGCTGTCGCTGGTCGAGCATGGTACTGAGTTCGTCATACGAGGGGAGAATGACATCTTCCAGCAGTTTTACATGTTGATa                            >  1:537700/1‑140 (MQ=255)                                                                                                         agcagGGTTGCATCACCCTGAACGGTGATTTCGGCGGCGCTCTCTGGCAATAGCAGGGTTTGCTGTCGCTGGTCGAGCATGGTACTGAGTTCGTCATACGAGGGGAGAATGACATCTTCCAGCAGTTTTACATGTTGATa                            >  1:1331628/1‑140 (MQ=255)                                                                                                              ggTTGCATCACCCTGAACGGTGATTTCGGCGGCGCTCTCTGGCAATAGCAGGGTTTGCTGTCGCTGGTCGAGCATGGTACTGAGTTCGTCATACGAGGGGAGAATGACATCTTCCAGCAGTTTTACa                                    <  1:302189/127‑1 (MQ=255)                                                                                                              ggTTGCATCACCCTGAACGGTGATTTCGGCGGCGCTCTCTGGCAATAGCAGGGTTTGCTGTCGCTGGTCGAGCATGGTACTGAGTTCGTCATACGAGGGGAGAATGACATCTTCCAGCAGTTTTACa                                    >  2:302189/1‑127 (MQ=255)                                                                                                              ggTTGCATCACCCTGAACGGTGATTTCGGCGGCGCTCTCTGGCAATAGCAGGGTTTGCTGTCGCTGGTCGAGCATGGTACTGAGTTCGTCATACGAGGGGAGAATGACATCTTCCAGCAGTTTTACATGTTGATAATTAc                       <  2:1344251/140‑1 (MQ=255)                                                                                                              ggTTGCATCACCCTGAACGGTGATTTCGGCGGCGCTCTCTGGCAATAGCAGGGTTTGCTGTCGCTGGTCGAGCATGGTACTGAGTTCGTCATACGAGGGGAGAATGACATCTTCCAGCAGTTTTACATGTTGATAATTAc                       >  1:467906/1‑140 (MQ=255)                                                                                                                 tGCATCACCCTGAACGGTGATTTCGGCGGCGCTCTCTGGCAATAGCAGGGTTTGCTGTCGCTGGTCGAGCATGGTACTGAGTTCGTCATACGAGGGGAGAATGACATCTTCCAGCAGTTTTACATGTTGATAATTACCgg                    <  1:607748/140‑1 (MQ=255)                                                                                                                 tGCATCACCCTGAACGGTGATTTCGGCGGCGCTCTCTGGCAATAGCAGGGTTTGCTGTCGCTGGTCGAGCATGGTACTGAGTTCGTCATACGAGGGGAGAATGACATCTTCCAGCAGTTTTACATGTTGATAATTACCgg                    <  2:467906/140‑1 (MQ=255)                                                                                                                      cACCCTGAACGGTGATTTCGGCGGCGCTCTCTGGCAATAGCAGGGTTTGCTGTCGCTGGTCGAGCATGGTACTGAGTTCGTCATACGAGGGGAGAATGACATCTTCCAGCAGTTTTACATGTTGATAATTACCGgaagaa               <  2:61766/140‑1 (MQ=255)                                                                                                                      cACCCTGAACGGTGATTTCGGCGGCGCTCTCTGGCAATAGCAGGGTTTGCTGTCGCTGGTCGAGCATGGTACTGAGTTCGTCATACGAGGGGAGAATGACATCTTCCAGCAGTTTTACATGTTGATAATTACCGgaagaa               <  2:537700/140‑1 (MQ=255)                                                                                                                       aCCCTGAACGGTGATTTCGGCGGCGCTCTCTGGCAATAGCAGGGTTTGCTGTCGCTGGTCGAGCATGGTACTGAGTTCGTCATACGAGGGGAGAATGACATCTTCCAGCAGTTTTACATGTTGATAATTACCGGAAGaaa              >  1:1403499/1‑140 (MQ=255)                                                                                                                        cccTGAACGGTGATTTCGGCGGCGCTCTCTGGCAATAGCAGGGTTTGCTGTCGCTGGTCGAGCATGGTACTGAGTTCGTCATACGAGGGGAGAATGACATCTTCCAGCAGTTTTACATGTTGATAATTACCGGAAGaaaa             >  1:100426/1‑140 (MQ=255)                                                                                                                           tGAACGGTGATTTCGGCGGCGCTCTCTGGCAATAGCAGGGTTTGCTGTCGCTGGTCGAGCATGGTACTGAGTTCGTCATACGAGGGGAGAATGACATCTTCCAGCAGTTTTACATGTTGATAATTACCGGAAGAAAATGa          <  2:552731/140‑1 (MQ=255)                                                                                                                           tGAACGGTGATTTCGGCGGCGCTCTCTGGCAATAGCAGGGTTTGCTGTCGCTGGTCGAGCATGGTACTGAGTTCGTCATACGAGGGGAGAATGACATCTTCCAGCAGTTTTACATGTTGATAATTACCGGAAGAAAATGa          <  2:1164115/140‑1 (MQ=255)                                                                                                                           tGAACGGTGATTTCGGCGGCGCTCTCTGGCAATAGCAGGGTTTGCTGTCGCTGGTCGAGCATGGTACTGAGTTCGTCATACGAGGGGAGAATGACATCTTCCAGCAGTTTTACATGTTGATAATTACCGGAAGAAAATGa          <  2:1403499/140‑1 (MQ=255)                                                                                                                           tGAACGGTGATTTCGGCGGCGCTCTCTGGCAATAGCAGGGTTTGCTGTCGCTGGTCGAGCATGGTACTGAGTTCGTCATACGAGGGGAGAATGACATCTTCCAGCAGTTTTACATGTTGATAATTACCGGAAGAAAATGa          <  2:100426/140‑1 (MQ=255)                                                                                                                            gAACGGTGATTTCGGCGGCGCTCTCTGGCAATAGCAGGGTTTGCTGTCGCTGGTCGAGCATGGTACTGAGTTCGTCATACGAGGGGAGAATGACATCTTCCAGCAGTTTTACATGTTGATAATTACCGGAAGAAAATGAc         <  2:229901/140‑1 (MQ=255)                                                                                                                                  tGATTTCGGCGGCGCTCTCTGGCAATAGCAGGGTTTGCTGTCGCTGGTCGAGCATGGTACTGAGTTCGTCATACGAGGGGAGAATGACATCTTCCAGCAGTTTTACATGTTGATAATTACCGGAAGAAAATGACTGTCCg   >  2:715226/1‑140 (MQ=255)                                                                                                                                   gATTTCGGCGGCGCTCTCTGGCAATAGCAGGGTTTGCTGTCGCTGGTCGAGCATGGTACTGAGTTCGTCATACGAGGGGAGAATGACATCTTCCAGCAGTTTTACATGTTGATAATTACCgg                    <  2:478935/122‑1 (MQ=255)                                                                                                                                   gATTTCGGCGGCGCTCTCTGGCAATAGCAGGGTTTGCTGTCGCTGGTCGAGCATGGTACTGAGTTCGTCATACGAGGGGAGAATGACATCTTCCAGCAGTTTTACATGTTGATAATTACCGGAAGAAAATGACTGTCCgg  >  1:1404463/1‑140 (MQ=255)                                                                                                                                      |                                                                                                                                         CTGCCATGACCGCTCCGTCATCTTCTTGCAGCTTAATCATAATGTTGCTGCCTTGCGGGCTGTAACGATGGGCGTTTTCTACCAGGTTTCGCAATAACATCCGCAGCAGGGTTGCATCACCCTGAACGGTGATGTCGGCGGCGCTCTCTGGCAATAGCAGGGTTTGCTGTCGCTGGTCGAGCATGGTACTGAGTTCGTCATACGAGGGGAGAATGACATCTTCCAGCAGTTTTACATGTTGATAATTACCGGAAGAAAATGACTGTCCGG  >  NC_000913/4332408‑4332677

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 24 ≤ ATCG/ATCG < 31 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑