Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	2,242	C→A	L636I (CTT→ATT)	thrA →	fused aspartate kinase/homoserine dehydrogenase 1

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	2,242	0	C	A	100.0%	213.9 / NA	59	L636I (CTT→ATT)	thrA	fused aspartate kinase/homoserine dehydrogenase 1
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base A (23/36);  total (23/36)

TCAGTTGCGTTATGCGGCGGAAAAATCGCGGCGTAAATTCCTCTATGACACCAACGTTGGGGCTGGATTACCGGTTATTGAGAACCTGCAAAATCTGCTCAATGCAGGTGATGAATTGATGAAGTTCTCCGGCATTCTTTCTGGTTCGCTTTCTTATATCTTCGGCAAGTTAGACGAAGGCATGAGTTTCTCCGAGGCGACCACGCTGGCGCGGGAAATGGGTTATACCGAACCGGACCCGCGAGATGATCTTTCTGGTATGGATGTG  >  NC_000913/2106‑2373                                                                                                                                         |                                                                                                                                    tCAGTTGCGTTATGCGGCGGAAAAATCGCGGCGTAAATTCCTCTATGACACCAACGTTGGGGCTGGATTACCGGTTATTGAGAACCTGCAAAATCTGCTCAATGCAGGTGATGAATTGATGAAGTTCTCCGGCAttattt                                                                                                                                  >  1:797884/1‑140 (MQ=255) tCAGTTGCGTTATGCGGCGAAAAAATCGCGGCGTAAATTCCTCTATGACACCAACGTTGGGGCTGGATTACCGGTTATTGAGAACCTGCAAAATCTGCTCAATGCAGGTGATGAATTGATGAAGTTCTCCGGCAttattt                                                                                                                                  >  1:118021/1‑140 (MQ=255)  cAGTTGCGTTATGCGGCGGAAAAATCGCGGCGTAAATTCCTCTATGACACCAACGTTGGGGCTGGATTACCGGTTATTGAGAACCTGCAAAATCTGCTCAATGCAGGTGATGAATTGATGAAGTTCTCCGGCAttatttc                                                                                                                                 >  2:781060/1‑140 (MQ=255)    gTTGCGTTATGCGGCGGAAAAATCGCGGCGTAAATTCCTCTATGACACCAACGTTGGGGCTGGATTACCGGTTATTGAGAACCTGCAAAATCTACTCAATGCAGGTGATGAATTGATGAAGTTCTCCGGCATTATTTCTg                                                                                                                               <  2:661181/140‑1 (MQ=255)     ttGCGTTATGCGGCGGAAAAATCGCGGCGTAAATTCCTCTATGACACCAACGTTGGGGCTGGATTACCGGTTATTGAGAACCTGCAAAATCTGCTCAATGCAGGTGATGAATTGATGAAGTTCTCCGGCATTATTTCTgg                                                                                                                              >  2:596640/1‑140 (MQ=255)      tGCGTTATGCGGCGGAAAAATCGCGGCGTAAATTCCTCTATGACACCAACGTTGGGGCTGGATTACCGGTTATTGAGAACCTGCAAAATCTGCTCAATGCAGGTGATGAATTGATGAAGTTCTCCGGCATTATTTCTGGt                                                                                                                             >  2:293818/1‑140 (MQ=255)        cGTTATGCGGCGGAAAAATCGCGGCGTAAATTCCTCTATGACACCAACGTTGGGGCTGGATTACCGGTTATTGAGAACCTGCAAAATCTGCTCAATGCAGGTGATGAATTGATGAAGTTCTCCGGCATTATTTCTGGTTc                                                                                                                           >  2:342571/1‑140 (MQ=255)         gTTATGCGGCGGAAAAATCGCGGCGTAAATTCCTCTATGACACCAACGTTGGGGCTGGATTACCGGTTATTGAGAACCTGCAAAATCTGCTCAATGCAGGTGATGAATTGATGAAGTTCTCCGGCATTATTTCTGGTTca                                                                                                                          <  1:781060/140‑2 (MQ=255)         gTTATGCGGCGGAAAAATCGCGGCGTAAATTCCTCTATGACACCAACGTTGGGACTGGATTACCGGTTATTGAGAACCTGCAAAATCTGCTCAATGCAGGTGATGAATTGATGAAGTTCTCCGGCATTATTTCTGGTTCg                                                                                                                          <  2:1100136/140‑1 (MQ=255)          ttATGCGGCGGAAAAATCGCGGCGTAAATTCCTCTATGACACCAACGTTGGGGCTGGATTACCGGTTATTGAGAACCTGCAAAATCTGCTCAATGCAGGTGATGAATTGATGAAGTTCTCCGGCATTATTTCTGGTTCg                                                                                                                          <  1:463484/139‑1 (MQ=255)          ttATGCGGCGGAAAAATCGCGGCGTAAATTCCTCTATGACACCAACGTTGGGGCTGGATTACCGGTTATTGAGAACCTGCAAAATCTGCTCAATGCAGGTGATGAATTGATGAAGTTCTCCGGCATTATTTCTGGTTCg                                                                                                                          >  2:463484/1‑139 (MQ=255)          ttATGCGGCGGAAAAATCGCGGCGTAAATTCCTCTATGACACCAACGTTGGGGCTGGATTACCGGTTATTGAGAACCTGCAAAATCTGCTCAATGCAGGTGATGAATTGATGAAGTTCTCCGGCATTATTTCTGGTTCGc                                                                                                                         <  1:1019060/140‑1 (MQ=255)          ttATGCGGCGGAAAAATCGCGGCGTAAATTCCTCTATGACACCAACGTTGGGGCTGGATTACCGGTTATTGAGAACCTGCAAAATCTGCTCAATGCAGGTGATGAATTGATGAAGTTCTCCGGCATTATTTCTGGTTCGc                                                                                                                         <  2:332972/140‑1 (MQ=255)          ttATGCGGCGGAAAAATCGCGGCGTAAATTCCTCTATGACACCAACGTTGGGGCTGGATTACCGGTTATTGAGAACCTGCAAAATCTGCTCAATGCAGGTGATGAATTGATGAAGTTCTCCGGCATTATTTCTGGTTCGc                                                                                                                         <  2:26096/140‑1 (MQ=255)          ttATGCGGCGGAAAAATCGCGGCGTAAATTCCTCTATGACACCAACGTTGGGGCTGGATTACCGGTTATTGAGAACCTGCAAAATCTGCTCAATGCAGGTGATGAATTGATGAAGTTCTCCGGCATTATTTCTGGTTCGc                                                                                                                         >  1:1154393/1‑140 (MQ=255)           tATGCGGCGGAAAAATCGCGGCGTAAATTCCTCTATGACACCAACGTTGGGGCTGGATTACCGGTTATTGAGAACCTGCAAAATCTGCTCAATGCAGGTGATGAATTGATGAAGTTCTCCGGCATTATTTCTGGTTCGCt                                                                                                                        <  2:1154393/140‑1 (MQ=255)           tATGCGGCGGAAAAATCGCGGCGTAAATTCCTCTATGACACCAACGTTGGGGCTGGATTACCGGTTATTGAGAACCTGCAAAATCTGCTCAATGCAGGTGATGAATTGATGAAGTTCTCCGGCATTATTTCTGGTTCGCt                                                                                                                        <  2:353595/140‑1 (MQ=255)            aTGCGGCGGAAAAATCGCGGCGTAAATTCCTCTATGACACCAACGTTGGGGCTGGATTACCGGTTATTGAGAACCTGCAAAATCTGCTCAATGCAGGTGATGAATTGATGAAGTTCTCCGGCATTATTTCTGGTTCGCtt                                                                                                                       <  2:1059260/140‑1 (MQ=255)                          tCGCGGCGTAAATTCCTCTATGACACCAACGTTGGGGCTGGATTACCGGTTATTGAGAACCTGCAAAATCTGCTCAATGCAGGTGATGAATTGATGAAGTTCTCCGGCATTATTTCTGGTTCGCTTTCTTATATCTTCgg                                                                                                         <  2:359373/140‑1 (MQ=255)                          tCGCGGCGTAAATTCCTCTATGACACCAACGTTGGGGCTGGATTACCGGTTATTGAGAACCTGCAAAATCTGCTCAATGCAGGTGATGAATTGATGAAGTTCTCCGGCATTATTTCTGGTTCGCTTTCTTATATCTTCgg                                                                                                         <  2:121453/140‑1 (MQ=255)                          tCGCGGCGTAAATTCCTCTATGACACCAACGTTGGGGCTGGATTACCGGTTATTGAGAACCTGCAAAATCTGCTCAATGCAGGTGATGAATTGATGAAGTTCTCCGGCATTATTTCTGGTTCGCTTTCTTATATCTTCgg                                                                                                         <  1:759401/140‑1 (MQ=255)                                  tAAATTCCTCTATGACACCAACGTTGGGGCTGGATTACCGGTTATTGAGAACCTGCAAAATCTGCTCAATGCAGGTGATGAATTGATGAAGTTCTCCGGCATTATTTCTGGTTCGCTTTCTTATATCTTCGGCAAGTTAg                                                                                                 >  2:713/1‑140 (MQ=255)                                   aaaTTCCTCTATGACACCAACGTTGGGGCTGGATTACCGGTTATTGAGAACCTGCAAAATCTGCTCAATGCAGGTGATGAATTGATGAAGTTCTCCGGCATTATTTCTGGTTCGCTTTCTTATATCTTCGGCAAGTTAGa                                                                                                <  1:290360/140‑1 (MQ=255)                                      ttCCTCTATGACACCAACGTTGGGGCTGGATTACCGGTTATTGAGAACCTGCAAAATCTGCTCAATGCAGGTGATGAATTGATGAAGTTCTCCGGCATTATTTCTGGTTCGCTTTCTTATATCTTCGGCAAGTTAGACGa                                                                                             <  1:293818/140‑1 (MQ=255)                                      ttCCTCTATGACACCAACGTTGGGGCTGGATTACCGGTTATTGAGAACCTGCAAAATCTGCTCAATGCAGGTGATGAATTGATGAAGTTCTCCGGCATTATTTCTGGTTCGCTTTCTTATATCTTCGGCAAGTTAGACGa                                                                                             <  2:797884/140‑1 (MQ=255)                                            tATGACACCAACGTTGGGGCTGGATTACCGGTTATTGAGAACCTGCAAAATCTGCTCAATGCAGGTGATGAATTGATGAAGTTCTCCGGCATTATTTCTGGTTCGCTTTCTTATATCTTCGGCAAGTTAGACGAAGGCAt                                                                                       <  2:118021/140‑1 (MQ=255)                                              tGACACCAACGTTGGGGCTGGATTACCGGTTATTGAGAACCTGCAAAATCTGCTCAATGCAGGTGATGAATTGATGAAGTTCTCCGGCATTATTTCTGGTTCGCTTTCTTATATCTTCGGCAAGTTAGACGAAGGCATGa                                                                                     >  2:1053592/1‑140 (MQ=255)                                               gACACCAACGTTGGGGCTGGATTACCGGTTATTGAGAACCTGCAAAATCTGCTCAATGCAGGTGATGAATTGATGAAGTTCTCCGGCATTATTTCTGGTTCGCTTTCTTATATCTTCGGCAAGTTAGACGAAGGCATGAg                                                                                    <  1:412509/140‑1 (MQ=255)                                                      aCGTTGGGGCTGGATTACCGGTTATTGAGAACCTGCAAAATCTGCTCAATGCAGGTGATGAATTGATGAAGTTCTCCGGCATTATTTCTGGTTCGCTTTCTTATATCTTCGGCAAGTTAGACGAAGGCATGAGTTTCTcc                                                                             >  2:435430/1‑140 (MQ=255)                                                      aCGTTGGGGCTGGATTACCGGTTATTGAGAACCTGCAAAATCTGCTCAATGCAGGTGATGAATTGATGAAGTTCTCCGGCATTATTTCTGGTTCGCTTTCTTATATCTTCGGCAAGTTAGACGAAGGCATGAGTTTCTcc                                                                             <  2:1170950/140‑1 (MQ=255)                                                      aCGTTGGGGCTGGATTACCGGTTATTGAGAACCTGCAAAATCTGCTCAATGCAGGGGATGAATTGATGAAGTTCTCCGGCATTATTTCTGGTTCGCTTTCTTATATCTTCGGCAAGTTAGACGAAGGCATGAGTTTCTcc                                                                             >  2:325242/1‑140 (MQ=255)                                                        gTTGGGGCTGGATTACCGGTTATTGAGAACCTGCAAAATCTGCTCAATGCAGGTGATGAATTGATGAAGTTCTCCGGCATTATTTCTGGTTCGCTTTCTTATATCTTCGGCAAGTTAGACGAAGGCATGAGTTTCTCCGa                                                                           >  2:1057920/1‑140 (MQ=255)                                                                  gATTACCGGTTATTGAGAACCTGCAAAATCTGCTCAATGCAGGTGATGAATTGATGAAGTTCTCCGGCATTATTTCTGGTTCGCTTTCTTATATCTTCGGCAAGTTAGACGAAGGCATGAGTTTCTCCGAGGCGACCACg                                                                 <  1:47835/140‑1 (MQ=255)                                                                         ggTTATTGAGAACCTGCAAAATCTGCTCAATGCAGGTGATGAATTGATGAAGTTCTCCGGCATTATTTCTGGTTCGCTTTCTTATATCTTCGGCAAGTTAGACGAAGGCATGAGTTTCTCCGAGGCGACCACGCTGgcgc                                                          <  1:13794/140‑1 (MQ=255)                                                                           ttATTGAGAACCTGCAAAATCTGCTCAATGCAGGTGATGAATTGATGAAGTTCTCCGGCATTATTTCTGGTTCGCTTTCTTATATCTTCGGCAAGTTAGACGAAGGCATGAGTTTCTCCGAGGCGACCACGCTGGCGCgg                                                        <  1:435430/140‑1 (MQ=255)                                                                              ttGAGAACCTGCAAAATCTGCTCAATGCAGGTGATGAATTGATGAAGTTCTCCGGCATTATTTCTGGTTCGCTTTCTTATATCTTCGGCAAGTTAGACGAAGGCATGAGTTTCTCCGAGGCGACCACGCTGGCGCGGGaa                                                     <  1:1053592/140‑1 (MQ=255)                                                                              ttGAGAACCTGCAAAATCTGCTCAATGCAGGTGATGAATTGATGAAGTTCTCCGGCATTATTTCTGGTTCGCTTTCTTATATCTTCGGCAAGTTAGACGAAGGCATGAGTTTCTCCGAGGCGACCACGCTGGCGCGGGaa                                                     <  1:1057920/140‑1 (MQ=255)                                                                              ttGAGAACCTGCAAAATCTGCTCAATGCAGGTGATGAATTGATGAAGTTCTCCGGCATTATTTCTGGTTCGCTTTCTTATATCTTCGGCAAGTTAGACGAAGGCATGAGTTTCTCCGAGGCGACCACGCTGGCGCGGGaa                                                     <  1:340057/140‑1 (MQ=255)                                                                              ttGAGAACCTGCAAAATCTGCTCAATGCAGGTGATGAATTGATGAAGTTCTCCGGCATTATTTCTGGTTCGCTTTCTTATATCTTCGGCAAGTTAGACGAAGGCATGAGTTTCTCCGAGGCGACCACGCTGGCGCGGGaa                                                     <  1:596640/140‑1 (MQ=255)                                                                                   aaCCTGCAAAATCTGCTCAATGCAGGTGATGAATTGATGAAGTTCTCCGGCATTATTTCTGGTTCGCTTTCTTATATCTTCGGCAAGTTAGACGAAGGCATGAGTTTCTCCGAGGCGACCACGCTGGCGCGGGAAATggg                                                <  1:713/140‑1 (MQ=255)                                                                                        gCAAAATCTGCTCAATGCAGGTGATGAATTGATGAAGTTCTCCGGCATTATTTCTGGTTCGCTTTCTTATATCTTCGGCAAGTTAGACg                                                                                              <  1:1251837/89‑1 (MQ=255)                                                                                        gCAAAATCTGCTCAATGCAGGTGATGAATTGATGAAGTTCTCCGGCATTATTTCTGGTTCGCTTTCTTATATCTTCGGCAAGTTAGACg                                                                                              >  2:1251837/1‑89 (MQ=255)                                                                                        gCAAAATCTGCTCAATGCAGGTGATGAATTGATGAAGTTCTCCGGCATTATTTCTGGTTCGCTTTCTTATATCTTCGGCAAGTTAGACGAAGGCATGAGTTTCTCCGAGGCGACCACGCTGGCGCGGGAAATGGGTtata                                           >  2:1260736/1‑140 (MQ=255)                                                                                         cAAAATCTGCTCAATGCAGGTGATGAATTGATGAAGTTCTCCGGCATTATTTCTGGTTCGCTTTCTTATATCTTCGGCAAGTTAGACGAAGGCATGAGTTTCTCCGAGGCGACCACGCTGGCGCGGGAAATGGGTTATAc                                          <  2:1261469/140‑1 (MQ=255)                                                                                                      aTGCAGGTGATGAATTGATGAAGTTCTCCGGCATTATTTCTGGTTCGCTTTCTTATATCTTCGGCAAGTTAGACGAAGGCATGAGTTTCTCCGAGGCGACCACGCTGGCGCGGGAAATGGGTTATACCGAACCGGACCcg                             >  1:846117/1‑140 (MQ=255)                                                                                                      aTGCAGGTGATGAATTGATGAAGTTCTCCGGCATTATTTCTGGTTCGCTTTCTTATATCTTCGGCAAGTTAGACGAAGGCATGAGTTTCTCCGAGGCGACCACGCTGGCGCGGGAAATGGGTTATACCGAACCGGACCcg                             <  1:342571/140‑1 (MQ=255)                                                                                                       tGCAGGTGATGAATTGATGAAGTTCTCCGGCATTATTTCTGGTTCGCTTTCTTATATCTTCGGCAAGTTAGACGAAGGCATGAGTTTCTCCGAGGCGACCACGCTGGCGCGGGAAATGGGTTATACCGAACCGGACCcgc                            >  2:1195045/1‑140 (MQ=255)                                                                                                          aGGTGATGAATTGATGAAGTTCTCCGGCATTATTTCTGGTTCGCTTTCTTATATCTTCGGCAAGTTAGACGAAGGCATGAGTTTCTCCGAGGCGACCACGCTGGCGCGGGAAATGGGTTATACCGAACCGGACCCGCgag                         <  1:1189829/140‑1 (MQ=255)                                                                                                            gTGATGAATTGATGAAGTTCTCCGGCATTATTTCTGGTTCGCTTTCTTATATCTTCGGCAAGTTAGACGAAGGCATGAGTTTCTCCGAGGCGACCACGCTGGCGCGGGAAATGGGTTATACCGAACCGGACCCGCGAgat                       >  2:881783/1‑140 (MQ=255)                                                                                                            gTGATGAATTGATGAAGTTCTCCGGCATTATTTCTGGTTCGCTTTCTTATATCTTCGGCAAGTTAGACGAAGGCATGAGTTTCTCCGAGGCGACCACGCTGGCGCGGGAAATGGGTTATACCGAACCGGACCCGCGAgat                       >  1:71965/1‑140 (MQ=255)                                                                                                               atgaATTGATGAAGTTCTCCGGCATTATTTCTGGTTCGCTTTCTTATATCTTCGGCAAGTTAGACGAAGGCATGAGTTTCTCCGAGGCGACCACGCTGGCGCGGGAAATGGGTTATACCGAACCGGACCCGCGAgatgat                    >  1:64533/1‑140 (MQ=255)                                                                                                               atgaATTGATGAAGTTCTCCGGCATTATTTCTGGTTCGCTTTCTTATATCTTCGGCAAGTTAGACGAAGGCATGAGTTTCTCCGAGGCGACCACGCTGGCGCGGGAAATGGGTTATACCGAACCGGACCCGCGAgatgat                    <  2:1170187/140‑1 (MQ=255)                                                                                                                 gaATTGATGAAGTTCTCCGGCATTATTTCTGGTTCGCTTTCTTATATCTTCGGCAAGTTAGACGAAGGCATGAGTTTCTCCGAGGCGACCACGCTGGCGCGGGAAATGGGTTATACCGAACCGGACCCGCGAGATGATCt                  >  2:625924/1‑140 (MQ=255)                                                                                                                   aTTGATGAAGTTCTCCGGCATTATTTCTGGTTCGCTTTCTTATATCTTCGGCAAGTTAGACGAAGGCATGAGTTTCTCCGAGGCGACCACGCTGGCGCGGGAAATGGGTTATACCGAACCGGACCCGCGAGATGATCttt                <  2:846117/140‑1 (MQ=255)                                                                                                                   aTTGATGAAGTTCTCCGGCATTATTTCTGGTTCGCTTTCTTATATCTTCGGCAAGTTAGACGAAGGCATGAGTTTCTCCGAGGCGACCACGCTGGCGCGGGAAATGGGTTATACCGAACCGGACCCGCGAGATGATCttt                >  2:895792/1‑140 (MQ=255)                                                                                                                   aTTGATGAAGTTCTCCGGCATTATTTCTGGTTCGCTTTCTTATATCTTCGGCAAGTTAGACGAAGGCATGAGTTTCTCCGAGGCGACCACGCTGGCGCGGGAAATGGGTTATACCGAACCGGACCCGCGAGATGATCttt                <  1:1169429/140‑1 (MQ=255)                                                                                                                    ttGATGAAGTTCTCCGGCATTATTTCTGGTTCGCTTTCTTATATCTTCGGCAAGTTAGACGAAGGCATGAGTTTCTCCGAGGCGACCACGCTGGCGCGGGAAATGGGTTATACCGAACCGGACCCGCGAGATGATCTTTc               >  2:1205812/1‑140 (MQ=255)                                                                                                                      gatgaAGTTCTCCGGCATTATTTCTGGTTCGCTTTCTTATATCTTCGGCAAGTTAGACGAAGGCATGAGTTTCTCCGAGGCGACCACGCTGGCGCGGGAAATGGGTTATACCGAACCGGACCCGCGAGATGATCTTTCTg             >  1:430794/1‑140 (MQ=255)                                                                                                                       atgaAGTTCTCCGGCATTATTTCTGGTTCGCTTTCTTATATCTTCGGCAAGTTAGACGAAGGCATGAGTTTCTCCGAGGCGACCACGCTGGCGCGGGAAATGGGTTATACCGAACCGGACCcgc                            <  1:304944/124‑1 (MQ=255)                                                                                                                       atgaAGTTCTCCGGCATTATTTCTGGTTCGCTTTCTTATATCTTCGGCAAGTTAGACGAAGGCATGAGTTTCTCCGAGGCGACCACGCTGGCGCGGGAAATGGGTTATACCGAACCGGACCcgc                            >  2:304944/1‑124 (MQ=255)                                                                                                                               ctcCGGCATTATTTCTGGTTCGCTTTCTTATATCTTCGGCAAGTTAGACGAAGGCATGAGTTTCTCCGAGGCGACCACGCTGGCGCGGGAAATGGGTTATACCGAACCGGACCCGCGAGATGATCTTTCTGGTATGGAtg    >  1:56650/1‑140 (MQ=255)                                                                                                                                 ccGGCATTATTTCTGGTTCGCTTTCTTATATCTTCGGCAAGTTAGACGAAGGCATGAGTTTCTCCGAGGCGACCACGCTGGCGCGGGAAATGGGTTATACCGAACCGGACCCGCGAGATGATCTTTCTGGTATGGAtgtg  <  2:594366/140‑1 (MQ=255)                                                                                                                                         |                                                                                                                                    TCAGTTGCGTTATGCGGCGGAAAAATCGCGGCGTAAATTCCTCTATGACACCAACGTTGGGGCTGGATTACCGGTTATTGAGAACCTGCAAAATCTGCTCAATGCAGGTGATGAATTGATGAAGTTCTCCGGCATTCTTTCTGGTTCGCTTTCTTATATCTTCGGCAAGTTAGACGAAGGCATGAGTTTCTCCGAGGCGACCACGCTGGCGCGGGAAATGGGTTATACCGAACCGGACCCGCGAGATGATCTTTCTGGTATGGATGTG  >  NC_000913/2106‑2373

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 28 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑