Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	2,058,358	C→A	G497C (GGC→TGC)	yeeO ←	FMN/FAD exporter

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	2,058,358	0	C	A	97.3%	258.1 / 0.3	75	G497C (GGC→TGC)	yeeO	FMN/FAD exporter
Reads supporting (aligned to +/- strand):  ref base C (2/0);  new base A (41/32);  total (43/32)
Fisher's exact test for biased strand distribution p-value = 5.04e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.74e-01

TTTTTTTCACACTTTTGCGGCTCGGGTCGAGGGTATTTCCATAGCCAACGTCCAGTAACCATTCGCCAGTAAAACAGCACGGCCCGCACAGCCCAGTCGGCAAACATTCCCATCCAGACACCAACCACACCCCAGCCAAGCATGATTCCCAGCACATAACCGACTACAACCCGACAACCCCACATGCTCAACATCGAAACCCACATGGCGTAACGGGCATCACGAGCACC‑TTTAAATCCAGCGGGTAGCACCCATGAGGCGGACCAAATAGGC  >  NC_000913/2058222‑2058494                                                                                                                                         |                                                                                                                                          tttttttCACACTTTTGCGGCTCGGGTCGAGGGTATTTCCATAGCCAACGTCCAGTAACCATTCGCCAGTAAAACAGCACGGCCCGCACAGCCCAGTCGGCAAACATTCCCATCCAGACACCAACCACACCCCAGCAAAg                                                                                                                                        >  1:376925/1‑140 (MQ=255)   tttttCACACTTTTGCGGCTCGGGTCGAGGGTATTTCCATAGCCAACGTCCAGTAACCATTCGCCAGTAAAACAGCACGGCCCGCACAGCCCAGTCGGCAAACATTCCCATCCAGACACCAACCACACCCCAGCAAAGCa                                                                                                                                      <  2:710237/140‑1 (MQ=255)         acacTTTTGCGGCTCGGGTCGAGGGTATTTCCATAGCCAACGTCCAGTAACCATTCGCCAGTAAAACAGCACGGCCCGCACAGCCCAGTCGGCAAACATTCCCATCCAGACACCAACCACACCCCAGCAAAGCATGATTc                                                                                                                                >  2:193176/1‑140 (MQ=255)          cacTTTTGCGGCTCGGGTCGAGGGTATTTCCATAGCCAACGTCCAGTAACCATTCGCCAGTAAAACAGCACGGCCCGCACAGCCCAGTCGGCAAACATTCCCATCCAGACACCGACCACACCCCAGCAAAGCATGATTcc                                                                                                                               <  1:361908/140‑1 (MQ=255)          cacTTTTGCGGCTCGGGTCGAGGGTATTTCCATAGCCAACGTCCAGTAACCATTCGCCAGTAAAACAGCACGGCCCGCACAGCCCAGTCGGCAAACATTCCCATCCAGACACCAACCACACCCCAGCAAAGCATGATTcc                                                                                                                               <  1:495729/140‑1 (MQ=255)          cacTTTTGCGGCTCGGGTCGAGGGTATTTCCATAGCCAACGTCCAGTAACCATTCGCCAGTAAAACAGCACGGCCCGCACAGCCCAGTCGGCAAACATTCCCATCCAGACACCAACCACACCCCAGCAAAGCATGATTcc                                                                                                                               <  1:629403/140‑1 (MQ=255)          cacTTTTGCGGCTCGGGTCGAGGGTATTTCCATAGCCAACGTCCAGTAACCATTCGCCAGTAAAACAGCACGGCCCGCACAGCCCAGTCGGCAAACATTCCCATCCAGACACCAACCACACCCCAGCAAAGCATGATTcc                                                                                                                               <  2:24987/140‑1 (MQ=255)                 gCGGCTCGGGTCGAGGGTATTTCCATAGCCAACGTCCAGTAACCATTCGCCAGTAAAACAGCACGGCCCGCACAGCCCAGTCGGCAAACATTCCCATCCAGACACCAACCACACCCCAGCAAAGCATGATTCCCAGcaca                                                                                                                        <  2:324117/140‑1 (MQ=255)                     cTCGGGTCGAGGGTATTTCCATAGCCAACGTCCAGTAACCATTCGCCAGTAAAACAGCACGGCCCGCACAGCCCAGTCGGCAAACATTCCCATCCAGACACCAACCACACCCCAGCAAAGCATGATTCCCAGCACATAAc                                                                                                                    <  2:196174/140‑1 (MQ=255)                     cTCGGGTCGAGGGTATTTCCATAGCCAACGTCCAGTAACCATTCGCCAGTAAAACAGCACGGCCCGCACAGCCCAGTCGGCAAACATTCCCATCCAGACACCAACCACACCCCAGCAAAGCATGATTCCCAGCACATAAc                                                                                                                    <  2:402047/140‑1 (MQ=255)                       cGGGTCGAGGGTATTTCCATAGCCAACGTCCAGTAACCATTCGCCAGTAAAACAGCACGGCCCGCACAGCCCAGTCGGCAAACATTCCCATCCAGACACCAACCACACCCCAGCAAAGCATGATTCCCAGCACATAACCg                                                                                                                  >  2:267594/1‑140 (MQ=255)                         ggTCGAGGGTATTTCCATAGCCAACGTCCAGTAACCATTCGCCAGTAAAACAGCACGGCCCGCACAGCCCAGTCGGCAAACATTCCCATCCAGACACCAACCACACCCCAGCAAAGCATGATTCCCAGCACATAACCGAc                                                                                                                <  2:1186347/140‑1 (MQ=255)                         ggTCGAGGGTATTTCCATAGCCAACGTCCAGTAACCATTCGCCAGTAAAACAGCACGGCCCGCACAGCCCAGTCGGCAAACATTCCCATCCAGACACCAACCACACCCCAGCAAAGCATGATTCCCAGCACATAACCGAc                                                                                                                >  1:636842/1‑140 (MQ=255)                            cGAGGGTATTTCCATAGCCAACGTCCAGTAACCATTCGCCAGTAAAACAGCACGGCCCGCACAGCCCAGTCGGCAAACATTCCCATCCAGACACCAACCACACCCCAGCAAAGCATGATTCCCAGCACATAACCGACTAc                                                                                                             <  1:1100576/140‑1 (MQ=255)                                    tttCCATAGCCAACGTCCAGTAACCATTCGCCAGTAAAACAGCACGGCCCGCACAGCCCAGTCGGCAAACATTCCCATCCAGACACCAACCACACCCCAGCAAAGCATGATTCCCAGCACATAACCGACTACAACCCGAc                                                                                                     >  2:937592/1‑140 (MQ=255)                                    tttCCATAGCCAACGTCCAGTAACCATTCGCCAGTAAAACAGCACGGCCCGCACAGCCCAGTCGGCAAACATTCCCATCCAGACACCAACCACACCCCAGCAAAGCATGATTCCCAGCACATAACCGACTACAACCCGAc                                                                                                     <  2:828147/140‑1 (MQ=255)                                     ttCCATAGCCAACGTCCAGTAACCATTCGCCAGTAAAACAGCACGGCCCGCACAGCCCAGTCGGCAAACATTCCCATCCAGACACCAACCACACCCCAGCAAAGCATGATTCCCAGCACATAACCGACTACAACCCGACa                                                                                                    <  2:376925/140‑1 (MQ=255)                                       ccATAGCCAACGTCCAGTAACCATTCGCCAGTAAAACAGCACGGCCCGCACAGCCCAGTCGGCAAACATTCCCATCCAGACACCAACCACACCCCGGCAAAGCATGATTCCCAGCACATAACCGACTACAACCCGACAAc                                                                                                  <  1:451463/140‑1 (MQ=255)                                       ccATAGCCAACGTCCAGTAACCATTCGCCAGTAAAACAGCACGGCCCGCACAGCCCAGTCGGCAAACATTCCCATCCAGACACCAACCACACCCCAGCAAAGCATGATTCCCAGCACATAACCGACTACAACCCGACAAc                                                                                                  <  1:958826/140‑1 (MQ=255)                                           aGCCAACGTCCAGTAACCATTCGCCAGTAAAACAGCACGGCCCGCACAGCCCAGTCGGCAAACATTCCCATCCAGACACCAACCACACCCCAGCAAAGCATGATTCCCAGCACATAACCGACTACAACCCGACAACCCca                                                                                              >  2:270572/1‑140 (MQ=255)                                               aaCGTCCAGTAACCATTCGCCAGTAAAACAGCACGGCCCGCACAGCCCAGTCGGCAAACATTCCCATCCAGACACCAACCACACCCCAGCAAAGCATGATTCCCAGCACATAACCGACTACAACCCGACAACCCCACATg                                                                                          >  1:191051/1‑140 (MQ=255)                                               aaCGTCCAGTAACCATTCGCCAGTAAAACAGCACGGCCCGCACAGCCCAGTCGGCAAACATTCCCATCCAGACACCAACCACACCCCAGCAAAGCATGATTCCCAGCACATAACCGACTACAACCCGACAACCCCACATg                                                                                          >  2:38441/1‑140 (MQ=255)                                                aCGTCCAGTAACCATTCGCCAGTAAAACAGCACGGCCCGCACAGCCCAGTCGGCAAACATTCCCATCCAGACACCAACCACACCCCAGCAAAGCATGATTCCCAGCACATAACCGACTACAACCCGACAACCCCACATGc                                                                                         >  1:667805/1‑140 (MQ=255)                                                 cGTCCAGTAACCATTCGCCAGTAAAACAGCACGGCCCGCACAGCCCAGTCGGCAAACATTCCCATCCAGACACCAACCACACCCCAGCAAAGCATGATTCCCAGCACATAACCGACTACAACCCGACAACCCCACATGCt                                                                                        >  1:341605/1‑140 (MQ=255)                                                 cGTCCAGTAACCATTCGCCAGTAAAACAGCACGGCCCGCACAGCCCAGTCGGCAAACATTCCCATCCAGACACCAACCACACCCCAGCAAAGCATGATTCCCAGCACATAACCGACTACAACCCGACAACCCCACATGCt                                                                                        >  2:1049954/1‑140 (MQ=255)                                                 cGTCCAGTAACCATTCGCCAGTAAAACAGCACGGCCCGCACAGCCCAGTCGGCAAACATTCCCATCCAGACACCAACCACACCCCAGCAAAGCATGATTCCCAGCACATAACCGACTACAACCCGACAACCCCACATGCt                                                                                        >  1:138647/1‑140 (MQ=255)                                                 cGTCCAGTAACCATTCGCCAGTAAAACAGCACGGCCCGCACAGCCCAGTCGGCAAACATTCCCATCCAGACACCAACCACACCCCAGCAAAGCATGATTCCCAGCACATAACCGACTACAACCCGACAACCCCACATGCt                                                                                        >  1:906295/1‑140 (MQ=255)                                                 cGTCCAGTAACCATTCGCCAGTAAAACAGCACGGCCCGCACAGCCCAGTCGGCAAACATTCCCATCCAGACACCAACCACACCCCAGCAAAGCATGATTCCCAGCACATAACCGACTACAACCCGACAACCCCACATGCt                                                                                        >  1:966756/1‑140 (MQ=255)                                                 cGTCCAGTAACCATTCGCCAGTAAAACAGCACGGCCCGCACAGCCCAGTCGGCAAACATTCCCATCCAGACACCAACCACACCCCAGCAAAGCATGATCCCCAGCACATAACCGACTACAACCCGACAACCCCACATGCt                                                                                        <  1:1114482/140‑1 (MQ=255)                                                   tCCAGTAACCATTCGCCAGTAAAACAGCACGGCCCGCACAGCCCAGTCGGCAAACATTCCCATCCAGACACCAACCACACCCCAGCAAAGCATGATTCCCAGCACATAACCGACTACAACCCGACAACCCCACATGCTCa                                                                                      <  2:527801/140‑1 (MQ=255)                                                      aGTAACCATTCGCCAGTAAAACAGCACGGCCCGCACAGCCCAGTCGGCAAACATTCCCATCCAGACACCAACCACACCCCAGCAAAGCATGATTCCCAGCACATAACCGACTACAACCCGACAACCCCACATGCTCAACa                                                                                   <  2:679950/140‑1 (MQ=255)                                                       gTAACCATTCGCCAGTAAAACAGCACGGCCCGCACAGCCCAGTCGGCAAACATTCCCATCCAGACACCAACCACACCCCAGCAAAGCATGATTCCCAGCACATAACCGACTACAACCCGACAACCCCACATGCTCAACAt                                                                                  >  1:479355/1‑140 (MQ=255)                                                             aTTCGCCAGTAAAACAGCACGGCCCGCACAGCCCAGTCGGCAAACATTCCCATCCAGACACCAACCACACCCCAGCCAAGCATGATTCCCAGCACATAACCGACTACAACCCGACAACCCCACATGCTCAACATCGAAAc                                                                            >  2:341696/1‑140 (MQ=255)                                                             aTTCGCCAGTAAAACAGCACGGCCCGCACAGCCCAGTCGGCAAACATTCCCATCCAGACACCAACCACACCCCAGCAAAGCATGATTCCCAGCACATAACCGACTACAACCCGACAACCCCACATGCTCAACATCGAAAc                                                                            <  2:12235/140‑1 (MQ=255)                                                                cGCCAGTAAAACAGCACGGCCCGCACAGCCCAGTCGGCAAACATTCCCATCCAGACACCAACCACACCCCAGCAAAGCATGATTCCCAGCACATAACCGACTACAACCCGACAACCCCACATGCTCAACATCGAAACCca                                                                         <  2:479355/140‑1 (MQ=255)                                                                 gCCAGTAAAACAGCACGGCCCGCACAGCCCAGTCGGCAAACATTCCCATCCAGACACCAACCACACCCCAGCAAAGCATGATTCCCAGCACATAACCGACTACAACCCGACAACCCCACATGCTCAACATCGAAACCcac                                                                        >  1:354830/1‑140 (MQ=255)                                                                  ccAGTAAAACAGCACGGCCCGCACAGCCCAGTCGGCAAACATTCCCATCCAGACACCAACCACACCCCAGCCAAGCATGATTCCCAGCACATAACCGACTACAACCCGACAACCCCACATGCTCAACATCGAAACCcaca                                                                       >  1:99197/1‑140 (MQ=255)                                                                  ccAGTAAAACAGCACGGCCCGCACAGCCCAGTCGGCAAACATTCCCATCCAGACACCAACCACACCCCAGCAAAGCATGATTCCCAGCACATAACCGACTACAACCCGACAACCCCACATGCTCAACATCGAAACCcaca                                                                       >  1:580091/1‑140 (MQ=255)                                                                  ccAGTAAAACAGCACGGCCCGCACAGCCCAGTCGGCAAACATTCCCATCCAGACACCAACCACACCCCAGCAAAGCATGATTCCCAGCACATAACCGACTACAACCCGACAACCCCACATGCTCAACATCGAAACCcaca                                                                       <  1:193176/140‑1 (MQ=255)                                                                      tAAAACAGCACGGCCCGCACAGCCCAGTCGGCAAACATTCCCATCCAGACACCAACCACACCCCAGCAAAGCATGATTCCCAGCACATAACCGACTACAACCCGACAACCCCACATGCTCAACATCGAAACCCACATGGc                                                                   >  1:929450/1‑140 (MQ=255)                                                                      tAAAACAGCACGGCCCGCACAGCCCAGTCGGCAAACATTCCCATCCAGACACCAACCACACCCCAGCAAAGCATGATTCCCAGCACATAACCGACTACAACCCGACAACCCCACATGCTCAACATCGAAACCCACATGGc                                                                   >  2:818050/1‑140 (MQ=255)                                                                          aCAGCACGGCCCGCACAGCCCAGTCGGCAAACATTCCCATCCAGACACCAACCACACCCCAGCAAAGCATGATTCCCAGCACATAACCGACTACAACCCGACAACCCCACATGCTCAACATCGAAACCCACATGGCGTaa                                                               <  1:38441/140‑1 (MQ=255)                                                                                cGGCCCGCACAGCCCAGTCGGCAAACATTCCCATCCAGACACCAACCACACCCCAGCAAAGCATGATTCCCAGCACATAACCGACTACAACCCGACAACCCCACATGCTCAACATCGAAACCCACATGGCGTAACGGGCa                                                         >  2:388274/1‑140 (MQ=255)                                                                                cGGCCCGCACAGCCCAGTCGGCAAACATTCCCATCCAGACACCAACCACACCCCAGCAAAGCATGATTCCCAGCACATAACCGACTACAACCCGACAACCCCACATGCTCAACATCGAAACCCACATGGCGTAACGGGCa                                                         >  2:41170/1‑140 (MQ=255)                                                                                cGGCCCGCACAGCCCAGTCGGCAAACATTCCCATCCAGACACCAACCACACCCCAGCAAAGCATGATTCCCAGCACATAACCGACTACAACCCGACAACCCCACATGCTCAACATCGAAACCCACATGGCGTAACGGGCa                                                         >  2:825324/1‑140 (MQ=255)                                                                                       cacaGCCCAGTCGGCAAACATTCCCATCCAGACACCAACCACACCCCAGCAAAGCATGATTCCCAGCACATAACCGACTACAACCCGACAACCCCACATGCTCAACATCGAAACCCACATGGCGTAACGGGCATCACGAg                                                  <  1:545263/140‑1 (MQ=255)                                                                                         caGCCCAGTCGGCAAACATTCCCATCCAGACACCAACCACACCCCAGCAAAGCATGATTCCCAGCACATAACCGACTACAACCCGACAACCCCACATGCTCAACATCGAAACCCACATGGCGTAACGGGCATCACGAGCa                                                <  1:41170/140‑1 (MQ=255)                                                                                           gCCCAGTCGGCAAACATTCCCATCCAGACACCAACCACACCCCAGCAAAGCATGATTCCCAGCACATAACCGACTACAACCCGACAACCCCACATGCTCAACATCGAAACCCACATGGCGTAACGGGCATCACGAGCAcc                                              >  1:333580/1‑140 (MQ=255)                                                                                           gCCCAGTCGGCAAACATTCCCATCCAGACACCAACCACACCCCAGCAAAGCATGATTCCCAGCACATAACCGACTACAACCCGACAACCCCACATGCTCAACATCGAAACCCACATGGCGTAACGGGCATCACGAGCAcc                                              >  2:811231/1‑140 (MQ=255)                                                                                            cccAGTCGGCAAACATTCCCATCCAGACACCAACCACACCCCAGCAAAGCATGATTCCCAGCACATAACCGACTACAACCCGACAACCCCACATGCTCAACATCGAAACCCACATGGCGTAACGGGCATCACGAGCACC‑t                                            >  1:882997/1‑140 (MQ=255)                                                                                            cccAGTCGGCAAACATTCCCATCCAGACACCAACCACACCCCAGCAAAGCATGATTCCCAGCACATAACCGACTACAACCCGACAACCCCACATGCTCAACATCGAAACCCACATGGCGTAACGGGCATCACGAGCACC‑t                                            >  1:845022/1‑140 (MQ=255)                                                                                                gTCGGCAAACATTCCCATCCAGACACCAACCACACCCCAGCAAAGCATGATTCCCAGCACATAACCGACTACAACCCGACAACCCCACATGCTCAACATCGAAACCCACATGGCGTAACGGGCATCACGAGCACC‑TTTaa                                        >  2:239151/1‑140 (MQ=255)                                                                                                    gCAAACATTCCCATCCAGACACCAACCACACCCCAGCAAAGCATGATTCCCAGCACATAACCGACTACAACCCGACAACCCCACATGCTCAACATCGAAACCCACATGGCGTAACGGGCATCACGAGCACC‑TTTAAATcc                                    >  2:36621/1‑140 (MQ=255)                                                                                                      aaaTATTCCCATCCAGACACCAACCACACCCCAGCAAAGCATGATTCCCAGCACATAACCGACTACAACCCGACAACCCCACATGCTCAACATCGAAACCCACATGGCGTAACGGGCATCACGAGCACC‑TTTAAATCCAg                                  >  1:982686/1‑140 (MQ=255)                                                                                                      aaaCATTCCCATCCAGACACCAACCACACCCCAGCAAAGCATGATTCCCAGCACATAACCGACTACAACCCGACAACCCCACATGCTCAACATCGAAACCCACATGGCGTAACGGGCATCACGAGCACC‑TTTaa                                        <  2:437420/134‑1 (MQ=255)                                                                                                      aaaCATTCCCATCCAGACACCAACCACACCCCAGCAAAGCATGATTCCCAGCACATAACCGACTACAACCCGACAACCCCACATGCTCAACATCGAAACCCACATGGCGTAACGGGCATCACGAGCACC‑TTTaa                                        >  1:437420/1‑134 (MQ=255)                                                                                                          aTTCCCATCCAGACACCAACCACACCCCAGCAAAGCATGATTCCCAGCACATAACCGACTACAACCCGACAACCCCACATGCTCAACATCGAAACCCACATGGCGTAACGGGCATCACGAGCACC‑TTTAAATCCAGCggg                              <  2:882997/140‑1 (MQ=255)                                                                                                             cccATCCAGACACCAACCACACCCCAGCAAAGCATGATTCCCAGCACATAACCGACTACAACCCGACAACCCCACATGCTCAACATCGAAACCCACATGGCGTAACGGGCATCACGAGCACC‑TTTAAATCCAGCGGGTAg                           <  1:811231/140‑1 (MQ=255)                                                                                                             cccATCCAGACACCAACCACACCCCAGCAAAGCATGATTCCCAGCACATAACCGACTACAACCCGACAACCCCACATGCTCAACATCGAAACCCACATGGCGTAACGGGCATCACGAGCACC‑TTTAAATCCAGCGGGTAg                           <  2:929450/140‑1 (MQ=255)                                                                                                               cATCCAGACACCAACCACACCCCAGCAAAGCATGATTCCCAGCACATAACCGACTACAACCCGACAACCCCACATGCTCAACATCGAAACCCACATGGCGTAACGGGCATCACGAGCACC‑TTTAAATCCAGCGGGTAGCa                         >  2:469549/1‑140 (MQ=255)                                                                                                                  ccAGACACCAACCACACCCCAGCAAAGCATGATTCCCAGCACATAACCGACTACAACCCGACAACCCCACATGCTCAACATCGAAACCCACATGGCGTAACGGGCATCACGAGCACC‑TTTAAATCCAGCGGGTAGCAccc                      >  1:1189016/1‑140 (MQ=255)                                                                                                                    aGACACCAACCACACCCCAGCAAAGCATGATTCCCAGCACATAACCGACTACAACCCGACAACCCCACATGCTCAACATCGAAACCCACATGGCGTAACGGGCATCACGAGCACC‑TTTAAATCCAGCGGGTAGCACCCAt                    >  2:1159486/1‑140 (MQ=255)                                                                                                                    aGACACCAACCACACCCCAGCAAAGCATGATTCCCAGCACATAACCGACTACAACCCGACAACCCCACATGCTCAACATCGAAACCCACATGGCGTAACGGGCATCACGAGCACC‑TTTAAATCCAGCGGGTAGCACCCAt                    >  2:662165/1‑140 (MQ=255)                                                                                                                    aGACACCAACCACACCCCAGCAAAGCATGATTCCCAGCACATAACCGACTACAACCCGACAACCCCACATGCTCAACATCGAAACCCACATGGCGTAACGGGCATCACGAGCACC‑TTTAAATCCAGCGGGTAGCACCCAt                    >  2:150732/1‑140 (MQ=255)                                                                                                                        accaaccaCACCCCAGCAAAGCATGATTCCCAGCACATAACCGACTACAACCCGACAACCCCACATGCTCAACATCGAAACCCACATGGCGTAACGGGCATCACGAGCACC‑TTTAAATCCAGCGGGTAGCACCCATGAgg                <  1:384914/140‑1 (MQ=255)                                                                                                                            accaCACCCCAGCAAAGCATGATTCCCAGCACATAACCGACTACAACCCGACAACCCCACATGCTCAACATCGAAACCCACATGGCGTAACGGGCATCACGAGCACCTTTTAAATCCAGCGGGTAGCACCCATGAGGCgg             >  2:579671/1‑140 (MQ=255)                                                                                                                            accaCACCCCAGCAAAGCATGATTCCCAGCACATAACCGACTACAACCCGACAACCCCACATGCTCAACATCGAAACCCACATGGCGTAACGGGCATCACGAGCACC‑TTTAAATCCAGCGGGTAGCACCCATGAGGCGGa            <  2:636842/140‑1 (MQ=255)                                                                                                                            accaCACCCCAGCAAAGCATGATTCCCAGCACATAACCGACTACAACCCGACAACCCCACATGCTCAACATCGAAACCCACATGGCGTAACGGGCATCACGAGCACC‑TTTAAATCCAGCGGGTAGCACCCATGAGGCGGa            >  2:1163064/1‑140 (MQ=255)                                                                                                                            accaCACCCCAGCAAAGCATGATTCCCAGCACATAACCGACTACAACCCGACAACCCCACATGCTCAACATCGAAACCCACATGGCGTAACGGGCATCACGAGCACC‑TTTAAATCCAGCGGGTAGCACCCATGAGGCGGa            >  1:1159639/1‑140 (MQ=255)                                                                                                                            accaCACCCCAGCAAAGCATGATTCCCAGCACATAACCGACTACAACCCGACAACCCCACATGCTCAACATCGAAACCCACATGGCGTAACGGGCATCACGAGCACC‑TTTAAATCCAGCGGGTAGCACCCATGAGGCGGa            <  2:963861/140‑1 (MQ=255)                                                                                                                             ccaCACCCCAGCAAAGCATGATTCCCAGCACATAACCGACTACAACCCGACAACCCCACATGCTCAACATCGAAACCCACATGGCGTAACGGGCATCACGAGCACC‑TTTAAATCCAGCGGGTAGCACCCATGAGGCGGAc           <  1:1049954/140‑1 (MQ=255)                                                                                                                               acacCCCAGCAAAGCATGATTCCCAGCACATAACCGACTACAACCCGACAACCCCACATGCTCAACATCGAAACCCACATGGCGTAACGGGCATCACGAGCACC‑TTTAAATCCAGCGGGTAGCACCCATGAGGCGGACCa         >  1:837792/1‑140 (MQ=255)                                                                                                                                cacCCCAGCAAAGCATGATTCCCAGCACATAACCGACTACAACCCGACAACCCCACATGCTCAACATCGAAACCCACATGGCGTAACGGGCATCACGAGCACC‑TTTAAATCCAGCGGGTAGCACCCATGAGGCGGACCaa        >  2:346976/1‑140 (MQ=255)                                                                                                                                  ccccAGCAAAGCATGATTCCCAGCACATAACCGACTACAACCCGACAACCCCACATGCTCAACATCGAAACCCACATGGCGTAACGGGCATCACGAGCACC‑TTTAAATCCAGCGGGTAGCACCCATGAGGCGGACCAAAt      >  1:99335/1‑140 (MQ=255)                                                                                                                                      aGCAAAGCATGATTCCCAGCACATAACCGACTACAACCCGACAACCCCACATGCTCAACATCGAAACCCACATGGCGTAACGGGCATCACGAGCACC‑TTTAAATCCAGCGGGTAGCACCCATGAGGCGGACCAAATAGGc  <  2:1189016/140‑1 (MQ=255)                                                                                                                                         |                                                                                                                                          TTTTTTTCACACTTTTGCGGCTCGGGTCGAGGGTATTTCCATAGCCAACGTCCAGTAACCATTCGCCAGTAAAACAGCACGGCCCGCACAGCCCAGTCGGCAAACATTCCCATCCAGACACCAACCACACCCCAGCCAAGCATGATTCCCAGCACATAACCGACTACAACCCGACAACCCCACATGCTCAACATCGAAACCCACATGGCGTAACGGGCATCACGAGCACC‑TTTAAATCCAGCGGGTAGCACCCATGAGGCGGACCAAATAGGC  >  NC_000913/2058222‑2058494

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 30 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑