Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	3,268,876	C→A	S154S (TCC→TCA)	yhaC →	uncharacterized protein YhaC

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	3,268,876	0	C	A	100.0%	215.7 / NA	60	S154S (TCC→TCA)	yhaC	uncharacterized protein YhaC
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base A (22/38);  total (22/38)

TAATGAACCGTTAAAAAACCAGGAAATATCCACAGGTAATATCAATATTAATTGCCCATTAAAAGATATTTACAATGAAATCAGGAGGTTAAACGTAATTTTTAGTTGTGGGACTGGAGATATCGTTGATCTATCCTCTCTGGACTTACGTAATGTCGATTTAGATTATTATGATTTCACAGATAAACATATGGCTAATACTATTTTAAATCCTTTTAAATTGAATTCAACAAATTTTACTAATGCCAACATGTTTCAGGTTAATTTTGTTA  >  NC_000913/3268741‑3269012                                                                                                                                        |                                                                                                                                         tAATGAACCGTTAAAAAACCAGGAAATATCCACAGGTAATATCAATATTAATTGCCCATTAAAAGATATTTACAATGAAATCAGGAGGTTAAACGTAATTTTTAGTTGTGGGACTGGAGATATCGTTGATCTATCatctc                                                                                                                                      <  2:438844/140‑1 (MQ=255) tAATGAACCATTAAAAAACCAGGAAATATCCACAGGTAATATCAATATTAATTGCCCATTAAAAGATATTTACAATGAAATCAGGAGGTTAAACGTAATTTTTAGTTGTGGGACTGGAGATATCGTTGATCTATCatctc                                                                                                                                      <  1:334918/140‑1 (MQ=255)   aTGAACCGTTAAAAAACCAGGAAATATCCACAGGTAATATCAATATTAATTGCCCATTAAAAGATATTTACAATGAAATCAGGAGGTTAAACGTAATTTTTAGTTGTGGGACTGGAGATATCGTTGATCTATCATCTCTg                                                                                                                                    <  2:338082/140‑1 (MQ=255)      aaCCGTTAAAAAACCAGGAAATATCCACAGGTAATATCAATATTAATTGCCCATTAAAAGATATTTACAATGAAATCAGGAGGTTAAACGTAATTTTTAGTTGTGGGACTGGAGATATCGTTGATCTATCATCTCTGGAc                                                                                                                                 <  1:156033/140‑1 (MQ=255)        ccGTTAAAAAACCAGGAAATATCCACAGGTAATATCAATATTAATTGCCCATTAAAAGATATTTACAATGAAATCAGGAGGTTAAACGTAATTTTTAGTTGTGGGACTGGAGATATCGTTGATCTATCATCTCTGGACtt                                                                                                                               >  2:122329/1‑140 (MQ=255)        ccGTTAAAAAACCAGGAAATATCCACAGGTAATATCAATATTAATTGCCCATTAAAAGATATTTACAATGAAATCAGGAGGTTAAACGTAATTTTTAGTTGTGGGACTGGAGATATCGTTGATCTATCATCTCTGGACtt                                                                                                                               >  1:940626/1‑140 (MQ=255)           ttAAAAAACCAGGAAATATCCACAGGTAATATCAATATTAATTGCCCATTAAAAGATATTTACAATGAAATCAGGAGGTTAAACGTAATTTTTAGTTGTGGGACTGGAGATATCGTTGATCTATCATCTCTGGACTTACg                                                                                                                            >  2:479029/1‑140 (MQ=255)                aaaCCAGGAAATATCCACAGGTAATATCAATATTAATTGCCCATTAAAAGATATTTACAATGAAATCAGGAGGTTAAACGTAATTTTTAGTTGTGGGACTGGAGATATCGTTGATCTATCATCTCTGGACTTACGTAATg                                                                                                                       <  1:466728/140‑1 (MQ=255)                 aaCCAGGAAATATCCACAGGTAATATCAATATTAATTGCCCATTAAAAGATATTTACAATGAAATCAGGAGGTTAAACGTAATTTTTAGTTGTGGGACTGGAGATATCGTTGATCTATCATCTCTGGACTTACGTAATGt                                                                                                                      <  1:64270/140‑1 (MQ=255)                     aGGAAATATCCACAGGTAATATCAATATTAATTGCCCATTAAAAGATATTTACAATGAAATCAGGAGGTTAAACGTAATTTTTAGTTGTGGGACTGGAGATATCGTTGATCTATCATCACTGGACTTACGTAATGTCGAt                                                                                                                  <  1:309559/140‑1 (MQ=255)                      ggAAATATCCACAGGTAATATCAATATTAATTGCCCATTAAAAGATATTTACAATGAAATCAGGAGGTTAAACGTAATTTTTAGTTGTGGGACTGGAGATATCGTTGATCTATCATCTCTGGACTTACGTAATGTCGAtt                                                                                                                 >  2:1247090/1‑140 (MQ=255)                      ggAAATATCCACAGGTAATATCAATATTAATTGCCCATTAAAAGATATTTACAATGAAATCAGGAGGTTAAACGTAATTTTTAGTTGTGGGACTGGAGATATCGTTGATCTATCATCTCTGGACTTACGTAATGTCGAtt                                                                                                                 <  1:636228/140‑1 (MQ=255)                       gAAATATCCACAGGTAATATCAATATTAATTGCCCATTAAAAGATATTTACAATGAAATCAGGAGGTTAAACGTAATTTTTAGTTGTGGGACTGGAGATATCGTTGATCTATCATCTCTGGACTTACGTAATGTCGAttt                                                                                                                <  1:834237/140‑1 (MQ=255)                              ccACAGGTAATATCAATATTAATTGCCCATTAAAAGATATTTACAATGAAATCAGGAGGTTAAACGTAATTTTTAGTTGTGGGACTGGAGATATCGTTGATCTATCATCTCTGGACTTACGTAATGTCGATTTAGattat                                                                                                         >  2:9689/1‑140 (MQ=255)                                acaGGTAATATCAATATTAATTGCCCATTAAAAGATATTTACAATGAAATCAGGAGGTTAAACGTAATTTTTAGTTGTGGGACTGGAGATATCGTTGATCTATCATCTCTGGACTTACGTAATGTCGATTTAGAttatta                                                                                                       <  2:956280/140‑1 (MQ=255)                                 caGGTAATATCAATATTAATTGCCCATTAAAAGATATTTACAATGAAATCAGGAGGTTAAACGTAATTTTTAGTTGTGGGACTGGAGATATCGTTGATCTATCATCTCTGGACTTACGTAATGTCGATTTAGATtattat                                                                                                      >  2:489847/1‑140 (MQ=255)                                 caGGTAATATCAATATTAATTGCCCATTAAAAGATATTTACAATGAAATCAGGAGGTTAAACGTAATTTTTAGTTGTGGGACTGGAGATATCGTTGATCTATCATCTCTGGACTTACGTAATGTCGATTTAGATtattat                                                                                                      <  1:489847/140‑1 (MQ=255)                                      aaTATCGATATTAATTGCCCATTAAAAGATATTTACAATGAAATCAGGAGGTTAAACGTAATTTTTAGTTGTGGGACTGGAGATATCGTTGATCTATCATCTCTGGACTTACGTAATGTCGATTTAGATTATTATGAttt                                                                                                 <  2:998838/140‑1 (MQ=255)                                       atatCAATATTAATTGCCCATTAAAAGATATTTACAATGAAATCAGGAGGTTAAACGTAATTTTTAGTTGTGGGACTGGAGATATCGTTGATCTATCATCTCTGGACTTACGTAATGTCGATTTAGATTATTATGATTTc                                                                                                <  2:33003/140‑1 (MQ=255)                                        tatCAATATTAATTGCCCATTAAAAGATATTTACAATGAAATCAGGAGGTTAAACGTAATTTTTAGTTGTGGGACTGGAGATATCGTTGATCTATCATCTCTGGACTTACGTAATGTCGATTTAGATTATTATGATTTca                                                                                               <  1:479029/140‑1 (MQ=255)                                        tatCAATATTAATTGCCCATTAAAAGATATTTACAATGAAATCAGGAGGTTAAACGTAATTTTTAGTTGTGGGACTGGAGATATCGTTGATCTATCATCTCTGGACTTACGTAATGTCGATTTAGATTATTATGATTTca                                                                                               <  2:768097/140‑1 (MQ=255)                                             atatTAATTGCCCATTAAAAGATATTTACAATGAAATCAGGAGGTTAAACGTAATTTTTAGTTGTGGGACTGGAGATATCGTTGATCTATCATCTCTGGACTTACGTAATGTCGATTTAGATTATTATGATTTCACAGAt                                                                                          <  2:472048/140‑1 (MQ=255)                                             atatTAATTGCCCATTAAAAGATATTTACAATGAAATCAGGAGGTTAAACGTAATTTTTAGTTGTGGGACTGGAGATATCGTTGATCTATCATCTCTGGACTTACGTAATGTCGATTTAGATTATTATGATTTCACAGAt                                                                                          <  1:122329/140‑1 (MQ=255)                                             atatTAATTGCCCATTAAAAGATATTTACAATGAAATCAGGAGGTTAAACGTAATTTTTAGTTGTGGGACTGGAGATATCGTTGATCTATCATCTCTGGACTTACGTAATGTCGATTTAGATTATTATGATTACACAGAt                                                                                          <  2:711705/140‑1 (MQ=255)                                              tatTAATTGCCCATTAAAAGATATTTACAATGAAATCAGGAGGTTAAACGTAATTTTTAGTTGTGGGACTGGAGATATCGTTGATCTATCATCTCTGGACTTACGTAATGTCGATTTAGATTATTATGATTTca                                                                                               <  2:352974/134‑1 (MQ=255)                                              tatTAATTGCCCATTAAAAGATATTTACAATGAAATCAGGAGGTTAAACGTAATTTTTAGTTGTGGGACTGGAGATATCGTTGATCTATCATCTCTGGACTTACGTAATGTCGATTTAGATTATTATGATTTca                                                                                               >  1:352974/1‑134 (MQ=255)                                              tatTAATTGCCCATTAAAAGATATTTACAATGAAATCAGGAGGTTAAACGTAATTTTTAGTTGTGGGACTGGAGATATCGTTGATCTATCATCTCTGGACTTACGTAATGTCGATTTAGATTATTATGATTTCACAGATa                                                                                         <  2:463697/140‑1 (MQ=255)                                                  aaTTGCCCATTAAAAGATATTTACAATGAAATCAGGAGGTTAAACGTAATTTTTAGTTGTGGGACTGGAGATATCGTTGATCTATCATCTCTGGACTTACGTAATGTCGATTTAGATTATTATGATTTCACAGATAAACa                                                                                     >  2:858517/1‑140 (MQ=255)                                                   aTTGCCCATTAAAAGATATTTACAATGAAATCAGGAGGTTAAACGTAATTTTTAGTTGTGGGACTGGAGATATCGTTGATCTATCATCTCTGGACTTACGTAATGTCGATTTAGATTATTATGATTTCACAGATAAACat                                                                                    <  2:940626/140‑1 (MQ=255)                                                   aTTGCCCATTAAAAGATATTTACAATGAAATCAGGAGGTTAAACGTAATTTTTAGTTGTGGGACTGGAGATATCGTTGATCTATCATCTCTGGACTTACGTAATGTCGATTTAGATTATTATGATTTCACAGATAAACat                                                                                    <  1:98482/140‑1 (MQ=255)                                                   aTTGCCCATTAAAAGATATTTACAATGAAATCAGGAGGTTAAACGTAATTTTTAGTTGTGGGACTGGAGATATCGTTGATCTATCATCTCTGGACTTACGTAATGTCGATTTAGATTATTATGATTTCACAGATAAACat                                                                                    <  1:1209877/140‑1 (MQ=255)                                                    ttGCCCATTAAAAGATATTTACAATGAAATCAGGAGGTTAAACGTAATTTTTAGTTGTGGGACTGGAGATATCGTTGATCTATCATCTCTGGACTTACGTAATGTCGATTTAGATTATTATGATTTCACAGATAAACata                                                                                   <  2:865945/140‑1 (MQ=255)                                                      gCCCATTAAAAGATATTTACAATGAAATCAGGAGGTTAAACGTAATTTTTAGTTGTGGGACTGGAGATATCGTTGATCTATCATCTCTGGACTTACGTAATGTCGATTTAGATTATTATGATTTCACAGATAAACATATg                                                                                 >  1:30647/1‑140 (MQ=255)                                                       cccATTAAAAGATATTTACAATGAAATCAGGAGGTTAAACGTAATTTTTAGTTGTGGGACTGGAGATATCGTTGATCTATCATCTCTGGACTTACGTAATGTCGATTTAGATTATTATGATTTCACAGATAAACATATgg                                                                                >  1:1041210/1‑140 (MQ=255)                                                          aTTAAAAGATATTTACAATGAAATCAGGAGGTTAAACGTAATTTTTAGTTGTGGGACTGGAGATATCGTTGATCTATCATCTCTGGACTTACGTAATGTCGATTTAGATTATTATGATTTCACAGATAAACATATGGCTa                                                                             >  1:1041004/1‑140 (MQ=255)                                                          aTTAAAAGATATTTACAATGAAATCAGGAGGTTAAACGTAATTTTTAGTTGTGGGACTGGAGATATCGTTGATCTATCATCTCTGGACTTACGTAATGTCGATTTAGATTATTATGATTTCACAGATAAACATATGGCTa                                                                             >  1:98796/1‑140 (MQ=255)                                                           ttAAAAGATATTTACAATGAAATCAGGAGGTTAAACGTAATTTTTAGTTGTGGGACTGGAGATATCGTTGATCTATCATCTCTGGACTTACGTAATGTCGATTTAGATTATTATGATTTCACAGATAAACATATGGCTaa                                                                            <  2:1041004/140‑1 (MQ=255)                                                            tAAAAGATATTTACAATGAAATCAGGAGGTTAAACGTAATTTTTAGTTGTGGGACTGGAGATATCGTTGATCTATCATCTCTGGACTTACGTAATGTCGATTTAGATTATTATGATTTCACAGATAAACATATGGCTAAt                                                                           >  2:525365/1‑140 (MQ=255)                                                             aaaaGATATTTACAATGAAATCAGGAGGTTAAACGTAATTTTTAGTTGTGGGACTGGAGATATCGTTGATCTATCATCTCTGGACTTACGTAATGTCGATTTAGATTATTATGATTTCACAGATAAACATATGGCTAATa                                                                          <  2:159828/140‑1 (MQ=255)                                                             aaaaGATATTTACAATGAAATCAGGAGGTTAAACGTAATTTTTAGTTGTGGGACTGGAGATATCGTTGATCTATCATCTCTGGACTTACGTAATGTCGATTTAGATTATTATGATTTCACAGATAAACATATGGCTAATa                                                                          <  1:889506/140‑1 (MQ=255)                                                              aaaGATATTTACAATGAAATCAGGAGGTTAAACGTAATTTTTAGTTGTGGGACTGGAGATATCGTTGATCTATCATCTCTGGACTTACGTAATGTCGATTTAGATTATTATGATTTCACAGATAAACATATGGCTAATAc                                                                         <  2:30647/140‑1 (MQ=255)                                                              aaaGATATTTACAATGAAATCAGGAGGTTAAACGTAATTTTTAGTTGTGGGACTGGAGATATCGTTGATCTATCATCTCTGGACTTACGTAATGTCGATTTAGATTATTATGATTTCACAGATAAACATATGGCTAATAc                                                                         <  2:34523/140‑1 (MQ=255)                                                                       tACAATGAAATCAGGAGGTTAAACGTAATTTTTAGTTGTGGGACTGGAGATATCGTTGATCTATCATCTCTGGACTTACGTAATGTCGATTTAGATTATTATGATTTCACAGATAAACATATGGCTAATACTATTTTaaa                                                                <  1:1169982/140‑1 (MQ=255)                                                                         cAATGAAATCAGGAGGTTAAACGTAATTTTTAGTTGTGGGACTGGAGATATCGTTGATCTATCATCTCTGGACTTACGTAATGTCGATTTAGATTATTATGATTTCACAGATAAACATATGGCTAATACTATTTTAAATc                                                              <  1:1247090/140‑1 (MQ=255)                                                                               aaTCAGGAGGTTAAACGTAATTTTTAGTTGTGGGACTGGAGATATCGTTGATCTATCATCTCTGGACTTACGTAATGTCGATTTAGATTATTATGATTTCACAGATAAACATATGGCTAATACTATTTTAAATCCTTTTa                                                        >  1:1168902/1‑140 (MQ=255)                                                                                        gTTAAACGTAATTTTTAGTTGTGGGACTGGAGATATCGTTGATCTATCATCTCTGGACTTACGTAATGTCGATTTAGATTATTATGATTTCACAGATAAACATATGGCTAATACTATTTTAAATCCTTTTAAATTGAAtt                                               >  1:1268608/1‑140 (MQ=255)                                                                                          tAAACGTAATTTTTAGTTGTGGGACTGGAGATATCGTTGATCTATCATCTCTGGACTTACGTAATGTCGATTTAGATTATTATGATTTCACAGATAAACATATGGCTAATACTATTTTAAATCCTTTTAAATTGAATTca                                             >  1:1121705/1‑140 (MQ=255)                                                                                           aaaCGTAATTTTTAGTTGTGGGACTGGAGATATCGTTGATCTATCATCTCTGGACTTACGTAATGTCGATTTAGATTATTATGATTTCACAGATAAACATATGGCTAATACTATTTTAAATCCTTTTAAATTGAATTcaa                                            <  2:1041210/140‑1 (MQ=255)                                                                                           aaaCGTAATTTTTAGTTGTGGGACTGGAGATATCGTTGATCTATCATCTCTGGACTTACGTAATGTCGATTTAGATTATTATGATTTCACAGATAAACATATGGCTAATACTATTTTAAATCCTTTTAAATTGAATTcaa                                            >  2:1130684/1‑140 (MQ=255)                                                                                                  aTTTTTAGTTGTGGGACTGGAGATATCGTTGATCTATCATCTCTGGACTTACGTAATGTCGATTTAGATTATTATGATTTCACAGATAAACATATGGCTAATACTATTTTAAATCCTTTTAAATTGaa                                                 <  1:100292/128‑1 (MQ=255)                                                                                                  aTTTTTAGTTGTGGGACTGGAGATATCGTTGATCTATCATCTCTGGACTTACGTAATGTCGATTTAGATTATTATGATTTCACAGATAAACATATGGCTAATACTATTTTAAATCCTTTTAAATTGaa                                                 >  2:100292/1‑128 (MQ=255)                                                                                                   tttttAGTTGTGGGACTGGAGATATCGTTGATCTATCATCTCTGGACTTACGTAATGTCGATTTAGATTATTATGATTTCACAGATAAACATATGGCTAATACTATTTTAAATCCTTTTAAATTGAATTCAACAAAtttt                                    >  2:1019265/1‑140 (MQ=255)                                                                                                      ttAGTTGTGGGACTGGAGATATCGTTGATCTATCATCTCTGGACTTACGTAATGTCGATTTAGATTATTATGATTTCACAGATAAACATATGGCTAATACTATTTTAAATCCTTTTAAATTGAATTCAACAAATTTTACt                                 <  1:1133296/140‑1 (MQ=255)                                                                                                           tgtgGGACTGGAGATATCGTTGATCTATCATCTCTGGACTTACGTAATGTCGATTTAGATTATTATGATTTCACAGATAAACATATGGCTAATACTATTTTAAATCCTTTTAAATTGAATTCAACAAATTTTACTAATGc                            <  2:52534/140‑1 (MQ=255)                                                                                                           tgtgGGACTGGAGATATCGTTGATCTATCATCTCTGGACTTACGTAATGTCGATTTAGATTATTATGATTTCACAGATAAACATATGGCTAATACTATTTTAAATCCTTTTAAATTGAATTCAACAAATTTTACTAATGc                            <  1:616964/140‑1 (MQ=255)                                                                                                                          atCGTTGATCTATCATCTCTGGACTTACGTAATGTCGATTTAGATTATTATGATTTCACAGATAAACATATGGCTAATACTATTTTAAATCCTTTTAAATTGAATTCAACAAATTTTACTAATGCCAACATGTTTCAGGt             >  2:973013/1‑140 (MQ=255)                                                                                                                              ttGATCTATCATCTCTGGACTTACGTAATGTCGATTTAGATTATTATGATTTCACAGATAAACATATGGCTAATACTATTTTAAATCCTTTTAAATTGAATTCAACAAATTTTACTAATGCCAACATGTTTCAGGTTAAt         <  1:525365/140‑1 (MQ=255)                                                                                                                              ttGATCTATCATCTCTGGACTTACGTAATGTCGATTTAGATTATTATGATTTCACAGATAAACATATGGCTAATACTATTTTAAATCCTTTTAAATTGAATTCAACAAATTTTACTAATGCCAACATGTTTCAGGTTAAt         <  1:1230708/140‑1 (MQ=255)                                                                                                                               tGATCTATCATCTCTGGACTTACGTAATGTCGATTTAGATTATTATGATTTCACAGATAAACATATGGCTAATACTATTTTAAATCCTTTTAAATTGAATTCAACAAATTTTACTAATGCCAACATGTTTCAGGTTAAtt        >  1:55619/1‑140 (MQ=255)                                                                                                                                gATCTATCATCTCTGGACTTACGTAATGTCGATTTAGATTATTATGATTTCACAGATAAACATATGGCTAATACTATTTTAAATCCTTTTAAATTGAATTCAACAAATTTTACTAATGCCAACATGTTTCAGGTTAAttt       >  2:82381/1‑140 (MQ=255)                                                                                                                                   cTATCATCTCTGGACTTACGTAATGTCGATTTAGATTATTATGATTTCACAGATAAACATATGGCTAATACTATTTTAAATCCTTTTAAATTGAATTCAACAAATTTTACTAATGCCAACATGTTTCAGGTTAATTTTGt    <  1:504321/140‑1 (MQ=255)                                                                                                                                   cTATCATCTCTGGACTTACGTAATGTCGATTTAGATTATTATGATTTCACAGATAAACATATGGCTAATACTATTTTAAATCCTTTTAAATTGAATTCAACAAATTTTACTAATGCCAACATGTTTCAGGTTAATTTTGt    <  2:1233446/140‑1 (MQ=255)                                                                                                                                     atcatcTCTGGACTTACGTAATGTCGATTTAGATTATTATGATTTCACAGATAAACATATGGCTAATACTATTTTAAATCCTTTTAAATTGAATTCAACAAATTTTACTAATGCCAACATGTTTCAGGTTAATTTTGTTa  >  2:46756/1‑140 (MQ=255)                                                                                                                                        |                                                                                                                                         TAATGAACCGTTAAAAAACCAGGAAATATCCACAGGTAATATCAATATTAATTGCCCATTAAAAGATATTTACAATGAAATCAGGAGGTTAAACGTAATTTTTAGTTGTGGGACTGGAGATATCGTTGATCTATCCTCTCTGGACTTACGTAATGTCGATTTAGATTATTATGATTTCACAGATAAACATATGGCTAATACTATTTTAAATCCTTTTAAATTGAATTCAACAAATTTTACTAATGCCAACATGTTTCAGGTTAATTTTGTTA  >  NC_000913/3268741‑3269012

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 29 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑