Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	411,344	G→T	R380S (CGC→AGC)	araJ ←	putative transport protein AraJ

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	411,344	0	G	T	100.0%	199.1 / NA	58	R380S (CGC→AGC)	araJ	putative transport protein AraJ
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base T (30/28);  total (30/28)

GGCCTGCAAAATGTGCCAGAACCGCGTAGGGCGGATAAGGCGTTCACGCCGCATCCGGCAATAAGTAATGAGCACCGAGACTATAACCTACCCCAGTGGTTTCGCCAGCACCGGAGTATCCGCCGCTTGCTGGCGCTTATAGCGACCATACAGCAGCAACGACGACATCGCAGCAAACGAAAGCAGGGCGGCAGGCAGCGCCACGTAATTATATGCCAGCCCCAGCGTCAGCATCATACCTCCGCAATATGCGCCGACGGCGCTACCG  >  NC_000913/411210‑411477                                                                                                                                       |                                                                                                                                      ggCCTGCAAAATGTGCCAGAACCGCGTAGGGCGGATAAGGCGTTCACGCCGCATCCGGCAATAAGTAATGAGCACCGAGACTATAACCTACCCCAGTGGTTTCGCCAGCACCGGAGTATCCGCCGCTTGCTGGCTCTtat                                                                                                                                  <  2:275385/140‑1 (MQ=255) ggCCTGCAAAATGTGCCAGAACCGCGTAGGGCGGATAAGGCGTTCACGCCGCATCCGGCAATAAGTAATGAGCACCGAGACTATAACCTACCCCAGTGGTTTCGCCAGCACCGGAGTATCCGCCGCTTGCTGGCTCTtat                                                                                                                                  <  2:1240402/140‑1 (MQ=255)      gCAAAATGTGCCAGAACCGCGTAGGGCGGATAAGGCGTTCACGCCGCATCCGGCAATAAGTAATGAGCACCGAGACTATAACCTACCCCAGTGGTTTCGCCAGCACCGGAGTATCCGCCGCTTGCTGGCTCTTATAGc                                                                                                                               >  1:573393/1‑138 (MQ=255)      gCAAAATGTGCCAGAACCGCGTAGGGCGGATAAGGCGTTCACGCCGCATCCGGCAATAAGTAATGAGCACCGAGACTATAACCTACCCCAGTGGTTTCGCCAGCACCGGAGTATCCGCCGCTTGCTGGCTCTTATAGc                                                                                                                               <  2:573393/138‑1 (MQ=255)      gCAAAATGTGCCAGAACCGCGTAGGGCGGATAAGGCGTTCACGCCGCATCCGGCAATAAGTAATGAGCACCGAGACTATAACCTACCCCAGTGGTTTCGCCAGCACCGGAGTATCCGCCGCTTGCTGGCTCTTATAGCGa                                                                                                                             <  1:562796/140‑1 (MQ=255)       cAAAATGTGCCAGAACCGCGTAGGGCGGATAAGGCGTTCACGCCGCATCCGGCAATAAGTAATGAGCACCGAGACTATAACCTACCCCAGTGGTTTCGCCAGCACCGGAGTATCCGCCGCTTGCTGGCTCTTATAGCGAc                                                                                                                            <  1:1236623/140‑1 (MQ=255)            tgtgCCAGAACCGCGTAGGGCGGATAAGGCGTTCACGCCGCATCCGGCAATAAGTAATGAGCACCGAGACTATAACCTACCCCAGTGGTTTCGCCAGCACCGGAGTATCCGCCGCTTGCTGGCTCTTATAGCGACCATAc                                                                                                                       >  1:355647/1‑140 (MQ=255)            tgtgCCAGAACCGCGTAGGGCGGATAAGGCGTTCACGCCGCATCCGGCAATAAGTAATGAGCACCGAGACTATAACCTACCCCAGTGGTTTCGCCAGCACCGGAGTATCCGCCGCTTGCTGGCTCTTATAGCGACCATAc                                                                                                                       >  2:692551/1‑140 (MQ=255)                ccAGAACCGCGTAGGGCGGATAAGGCGTTCACGCCGCATCCGGCAATAAGTAATGAGCACCGAGACTATAACCTACCCCAGTGGTTTCGCCAGCACCGGAGTATCCGCCGCTTGCTGGCTCTTATAGCGACCATAcagca                                                                                                                   <  2:1123398/140‑1 (MQ=255)                ccAGAACCGCGTAGGGCGGATAAGGCGTTCACGCCGCATCCGGCAATAAGTAATGAGCACCGAGACTATAACCTACCCCAGTGGTTTCGCCAGCACCGGAGTATCCGCCGCTTGCTGGCTCTTATAGCGACCATAcagca                                                                                                                   >  1:914039/1‑140 (MQ=255)                         cgTAGGGCGGATAAGGCGTTCACGCCGCATCCGGCAATAAGTAATGAGCACCGAGACTATAACCTACCCCAGTGGTTTCGCCAGCACCGGAGTATCCGCCGCTTGCTGGCTCTTATAGCGACCATACAGCAGCAacgacg                                                                                                          >  1:150585/1‑140 (MQ=255)                         cgTAGGGCGGATAAGGCGTTCACGCCGCATCCGGCAATAAGTAATGAGCACCGAGACTATAACCTACCCCAGTGGTTTCGCCAGCACCGGAGTATCCGCCGCTTGCTGGCTCTTATAGCGACCATACAGCAGCAacgacg                                                                                                          >  1:1130766/1‑140 (MQ=255)                                              aCGCCGCATCCGGCAATAAGTAATGAGCACCGAGACTATAACCTACCCCAGTGGTTTCGCCAGCACCGGAGTATCCGCCGCTTGCTGGCTCTTATAGCGACCATACAGCAGCAACGACGACATCGCAGCAAACGAAAGCa                                                                                     <  1:45938/140‑1 (MQ=255)                                                           cAATAAGTAATGAGCACCGAGACTATAACCTACCCCAGTGGTTTCGCCAGCACCGGAGTATCCGCCGCTTGCTGGCTCTTATAGCGACCATACAGCAGCAACGACGACATCGCAGCAAACGAAAGCAGGGCGgcaggcag                                                                        <  2:370265/140‑1 (MQ=255)                                                                aGTAATGAGCACCGAGACTATAACCTACCCCAGTGGTTTCGCCAGCACCGGAGTATCCGCCGCTTGCTGGCTCTTATAGCGACCATACAGCAGCAACGACGACATCGCAGCAAACGAAAGCAGGGCGGCAGGCAGCGCCa                                                                   <  2:323741/140‑1 (MQ=255)                                                                aGTAATGAGCACCGAGACTATAACCTACCCCAGTGGTTTCGCCAGCACCGGAGTATCCGCCGCTTGCTGGCTCTTATAGCGACCATACAGCAGCAACGACGACATCGCAGCAAACGAAAGCAGGGCGGCAGGCAGCGCCa                                                                   <  2:1023741/140‑1 (MQ=255)                                                                  tAATGAGCACCGAGACTATAACCTACCCCAGTGGTTTCGCCAGCACCGGAGTATCCGCCGCTTGCTGGCTCTTATAGCGACCATACAGCAGCAACGACGACATCGCAGCAAACGAAAGCAGGGCGGCAGGCAGCGCCACg                                                                 <  1:995302/140‑1 (MQ=255)                                                                   aaTGAGCACCGAGACTATAACCTACCCCAGTGGTTTCGCCAGCACCGGAGTATCCGCCGCTTGCTGGCTCTTATAGCGACCATACAGCAGCAACGACGACATCGCAGCAAACGAAAGCAGGGCGGCAGGCAGCGCCACGt                                                                <  2:382046/140‑1 (MQ=255)                                                                     tGAGCACCGAGACTATAACCTACCCCAGTGGTTTCGCCAGCACCGGAGTATCCGCCGCTTGCTGGCTCTTATAGCGACCATACAGCAGCAACGACGACATCGCAGCAAACGAAAGCAGGGCGGCAGGCAGCGCCACGTaa                                                              >  2:13454/1‑140 (MQ=255)                                                                     tGAGCACCGAGACTATAACCTACCCCAGTGGTTTCGCCAGCACCGGAGTATCCGCCGCTTGCTGGCTCTTATAGCGACCATACAGCAGCAACGACGACATCGCAGCAAACGAAAGCAGGGCGGCAGGCAGCGCCACGTaa                                                              >  1:885282/1‑140 (MQ=255)                                                                       aGCACCGAGACTATAACCTACCCCAGTGGTTTCGCCAGCACCGGAGTATCCGCCGCTTGCTGGCTCTTATAGCGACCATACAGCAGCAACGACGACATCGCAGCAAACGAAAGCAGGGCGGCAGGCAGCGCCACGTAAtt                                                            >  1:560527/1‑140 (MQ=255)                                                                         cACCGAGACTATAACCTACCCCAGTGGTTTCGCCAGCACCGGAGTATCCGCCGCTTGCTGGCTCTTATAGCGACCATACAGCAGCAACGACGACATCGCAGCAAACGAAAGCAGGGCGGCAGGCAGCGCCACGTAATtat                                                          >  1:329217/1‑140 (MQ=255)                                                                         cACCGAGACTATAACCTACCCCAGTGGTTTCGCCAGCACCGGAGTATCCGCCGCTTGCTGGCTCTTATAGCGACCATACAGCAGCAACGACGACATCGCAGCAAACGAAAGCAGGGCGGCAGGCAGCGCCACGTAATtat                                                          >  2:206000/1‑140 (MQ=255)                                                                         cACCGAGACTATAACCTACCCCAGTGGTTTCGCCAGCACCGGAGTATCCGCCGCTTGCTGGCTCTTATAGCGACCATACAGCAGCAACGACGACATCGCAGCAAACGAAAGCAGGGCGGCAGGCAGCGCCACGTAATtat                                                          >  2:1106667/1‑140 (MQ=255)                                                                          aCCGAGACTATAACCTACCCCAGTGGTTTCGCCAGCACCGGAGTATCCGCCGCTTGCTGGCTCTTATAGCGACCATACAGCAGCAACGACGACATCGCAGCAAACGAAAGCAGGGCGGCAGGCAGCGCCACGTAATtata                                                         >  1:782986/1‑140 (MQ=255)                                                                            cGAGACTATAACCTACCCCAGTGGTTTCGCCAGCACCGGAGTATCCGCCGCTTGCTGGCTCTTATAGCGACCATACAGCAGCAACGACGACATCGCAGCAAACGAAAGCAGGGCGGCAGGCAGCGCCACGTAATTATATg                                                       >  1:452062/1‑140 (MQ=255)                                                                            cGAGACTATAACCTACCCCAGTGGTTTCGCCAGCACCGGAGTATCCGCCGCTTGCTGGCTCTTATAGCGACCATACAGCAGCAACGACGACATCGCAGCAAACGAAAGCAGGGCGGCAGGCAGCGCCACGTAATTATATg                                                       >  2:879190/1‑140 (MQ=255)                                                                            cGAGACTATAACCTACCCCAGTGGTTTCGCCAGCACCGGAGTATCCGCCCCTTGCTGGCTCTTATAGCGACCATACAGCAGCAACGACGACATCGCAGCAAACGAAAGCAGGGCGGCAGGCAGCGCCACGTAATTATATg                                                       >  1:1009739/1‑140 (MQ=255)                                                                                  tataACCTACCCCAGTGGTTTCGCCAGCACCGGAGTATCCGCCGCTTGCTGGCTCTTATAGCGACCATACAGCAGCAACGACGACATCGCAGCAAACGAAAGCAGGGCGGCAGGCAGCGCCACGTAATTATATGCCAGcc                                                 >  1:627825/1‑140 (MQ=255)                                                                                  tataACCTACCCCAGTGGTTTCGCCAGCACCGGAGTATCCGCCGCTTGCTGGCTCTTATAGCGACCATACAGCAGCAACGACGACATCGCAGCAAACGAAAGCAGGGCGGCAGGCAGCGCCACGTAATTATATGCCAGcc                                                 >  1:156571/1‑140 (MQ=255)                                                                                    taACCTACCCCAGTGGTTTCGCCAGCACCGGAGTATCCGCCGCTTGCTGGCTCTTATAGCGACCATACAGCAGCAACGACGACATCGCAGCAAACGAAAGCAGGGCGGCAGGCAGCGCCACGTAATTATATGCCAGcccc                                               <  1:1104953/140‑1 (MQ=255)                                                                                         tACCCCAGTGGTTTCGCCAGCACCGGAGTATCCGCCGCTTGCTGGCTCTTATAGCGACCATACAGCAGCAACGACGACATCGCAGCAAACGAAAGCAGGGCGGCAGGCAGCGCCACGTAATTATATGCCAGCCCCAGCGt                                          <  2:914039/140‑1 (MQ=255)                                                                                         tACCCCAGTGGTTTCGCCAGCACCGGAGTATCCGCCGCTTGCTGGCTCTTATAGCGACCATACAGCAGCAACGACGACATCGCAGCAAACGAAAGCAGGGCGGCAGGCAGCGCCACGTAATTATATGCCAGCCCCAGCGt                                          <  2:782986/140‑1 (MQ=255)                                                                                          aCCCCAGTGGTTTCGCCAGCACCGGAGTATCCGCCGCTTGCTGGCTCTTATAGCGACCATACAGCAGCAACGACGACATCGCAGCAAACGAAAGCAGGGCGGCAGGCAGCGCCACGTAATTATATGCCAGCCCCAGCGTc                                         >  1:439481/1‑140 (MQ=255)                                                                                          aCCCCAGTGGTTTCGCCAGCACCGGAGTATCCGCCGCTTGCTGGCTCTTATAGCGACCATACAGCAGCAACGACGACATCGCAGCAAACGAAAGCAGGGCGGCAGGCAGCGCCACGTAATTATATGCCAGCCCCAGCGTc                                         >  2:867145/1‑140 (MQ=255)                                                                                          aCCCCAGTGGTTTCGCCAGCACCGGAGTATCCGCCGCTTGCTGGCTCTTATAGCGACCATACAGCAGCAACGACGACATCGCAGCAAACGAAAGCAGGGCGGCAGGCAGCGCCACGTAATTATATGCCAGCCCCAGCGTc                                         >  2:67693/1‑140 (MQ=255)                                                                                          aCCCCAGTGGTTTCGCCAGCACCGGAGTATCCGCCGCTTGCTGGCTCTTATAGCGACCATACAGCAGCAACGACGACATCGCAGCAAACGAAAGCAGGGCGGCAGGCAGCGCCACGTAATTATATGCCAGCCCCAGCGTc                                         <  2:560527/140‑1 (MQ=255)                                                                                          aCCCCAGTGGTTTCGCCAGCACCGGAGTATCCGCCGCTTGCTGGCTCTTATAGCGACCATACAGCAGCAACGACGACATCGCAGCAAACGAAAGCAGGGCGGCAGGCAGCGCCACGTAATTATATGCCAGCCCCAGCGTc                                         <  2:156571/140‑1 (MQ=255)                                                                                          aCCCCAGTGGTTTCGCCAGCACCGGAGTATCCGCCGCTTGCTGGCTCTTATAGCGACCATACAGCAGCAACGACGACATCGCAGCAAACGAAAGCAGGGCGGCAGGCAGCGCCACGTAATTATATGCCAGCCCCAGCGTc                                         >  2:1150509/1‑140 (MQ=255)                                                                                          aCCCCAGTGGTTTCGCCAGCACCGGAGTATCCGCCGCTTGCTGGCTCTTATAGCGACCATACAGCAGCAACGACGACATCGCAGCAAACGAAAGCAGGGCGGCAGGCAGCGCCACGTAATTATATGCCAGCCCCAGCGTc                                         <  2:1044198/140‑1 (MQ=255)                                                                                            cccAGTGGTTTCGCCAGCACCGGAGTATCCGCCGCTTGCTGGCTCTTATAGCGACCATACAGCAGCAACGACGACATCGCAGCAAACGAAAGCAGGGCGGCAGGCAGCGCCACGTAATTATATGCCAGccc                                                >  1:355708/1‑131 (MQ=255)                                                                                            cccAGTGGTTTCGCCAGCACCGGAGTATCCGCCGCTTGCTGGCTCTTATAGCGACCATACAGCAGCAACGACGACATCGCAGCAAACGAAAGCAGGGCGGCAGGCAGCGCCACGTAATTATATGCCAGccc                                                <  2:355708/131‑1 (MQ=255)                                                                                            cccAGTGGTTTCGCCAGCACCGGAGTATCCGCCGCTTGCTGGCTCTTATAGCGACCATACAGCAGCAACGACGACATCGCAGCAAACGAAAGCAGGGCGGCAGGCAGCGCCACGTAATTATATGCCAGCCCCAGCGTc                                         <  2:206945/138‑1 (MQ=255)                                                                                            cccAGTGGTTTCGCCAGCACCGGAGTATCCGCCGCTTGCTGGCTCTTATAGCGACCATACAGCAGCAACGACGACATCGCAGCAAACGAAAGCAGGGCGGCAGGCAGCGCCACGTAATTATATGCCAGCCCCAGCGTc                                         >  1:206945/1‑138 (MQ=255)                                                                                            cccAGTGGTTTCGCCAGCACCGGAGTATCCGCCGCTTGCTGGCTCTTATAGCGACCATACAGCAGCAACGACGACATCGCAGCAAACGAAAGCAGGGCGGCAGGCAGCGCCACGTAATTATATGCCAGCCCCAGCGTCac                                       >  1:191332/1‑139 (MQ=255)                                                                                            cccAGTGGTTTCGCCAGCACCGGAGTATCCGCCGCTTGCTGGCTCTTATAGCGACCATACAGCAGCAACGACGACATCGCAGCAAACGAAAGCAGGGCGGCAGGCAGCGCCACGTAATTATATGCCAGCCCCAGCGTCAg                                       <  2:1130766/140‑1 (MQ=255)                                                                                                gTGGTTTCGCCAGCACCGGAGTATCCGCCGCTTGCTGGCTCTTATAGCGACCATACAGCAGCAACGACGACATCGCAGCAAACGAAAGCAGGGCGGCAGGCAGCGCCACGTAATTATATGCCAGCCCCAGCGTCAGcatc                                   >  1:5716/1‑140 (MQ=255)                                                                                                gTGGTTTCGCCAGCACCGGAGTATCCGCCGCTTGCTGGCTCTTATAGCGACCATACAGCAGCAACGACGACATCGCAGCAAACGAAAGCAGGGCGGCAGGCAGCGCCACGTAATTATATGCCAGCCCCAGCGTCAGcatc                                   >  2:1009932/1‑140 (MQ=255)                                                                                                 tGGTTTCGCCAGCACCGGAGTATCCGCCGCTTGCTGGCTCTTATAGCGACCATACAGCAGCAACGACGACATCGCAGCAAACGAAAGCAGGGCGGCAGGCAGCGCCACGTAATTATATGCCAGCCCCAGCGTCAGcatca                                  <  1:13454/140‑1 (MQ=255)                                                                                                 tGGTTTCGCCAGCACCGGAGTATCCGCCGCTTGCTGGCTCTTATAGCGACCATACAGCAGCAACGACGACATCGCAGCAAACGAAAGCAGGGCGGCAGGCAGCGCCACGTAATTATATGCCAGCCCCAGCGTCAGcatca                                  <  1:277723/140‑1 (MQ=255)                                                                                                 tGGTTTCGCCAGCACCGGAGTATCCGCCGCTTGCTGGCTCTTATAGCGACCATACAGCAGCAACGACGACATCGCAGCAAACGAAAGCAGGGCGGCAGGCAGCGCCACGTAATTATATGCCAGCCCCAGCGTCAGcatca                                  >  1:694865/1‑140 (MQ=255)                                                                                                     ttCGCCAGCACCGGAGTATCCGCCGCTTGCTGGCTCTTATAGCGACCATACAGCAGCAACGACGACATCGCAGCAAACGAAAGCAGGGCGGCAGGCAGCGCCACGTAATTATATGCCAGCCCCAGCGTCAGCATCATAcc                              >  1:232600/1‑140 (MQ=255)                                                                                                                    gTATCCGCCGCTTGCTGGCTCTTATAGCGACCATACAGCAGCAACGACGACATCGCAGCAAACGAAAGCAGGGCGGCAGGCAGCGCCACGTAATTATATGCCAGCCCCAGCGTCAGCATCATACCTCCGCAATATGCGcc               <  1:692551/140‑1 (MQ=255)                                                                                                                      aTCCGCCGCTTGCTGGCTCTTATAGCGACCATACAGCAGCAACGACGACATCGCAGCAAACGAAAGCAGGGCGGCAGGCAGCGCCACGTAATTATATGCCAGCCCCAGCGTCAGCATCATACCTCCGCAATATGCGCCGa             <  2:694865/140‑1 (MQ=255)                                                                                                                      aTCCGCCGCTTGCTGGCTCTTATAGCGACCATACAGCAGCAACGACGACATCGCAGCAAACGAAAGCAGGGCGGCAGGCAGCGCCACGTAATTATATGCCAGCCCCAGCGTCAGCATCATACCTCCGCAATATGCGCCGa             <  1:67693/140‑1 (MQ=255)                                                                                                                            cgcTTGCTGGCTCTTATAGCGACCATACAGCAGCAACGACGACATCGCAGCAAACGAAAGCAGGGCGGCAGGCAGCGCCACGTAATTATATGCCAGCCCCAGCGTCAGCATCATACCTCCGCAATATGCGCCGACGgcgc       >  1:762834/1‑140 (MQ=255)                                                                                                                                tGCTGGCTCTTATAGCGACCATACAGCAGCAACGACGACATCGCAGCAAACGAAAGCAGGGCGGCAGGCAGCGCCACGTAATTATATGCCAGCCCCAGCGTCAGCATCATACCTCCGCAATATGCGCCGACGGCGCTAcc   <  1:867145/140‑1 (MQ=255)                                                                                                                                 gCTGGCTCTTATAGCGACCATACAGCAGCAACGACGACATCGCAGCAAACGAAAGCAGGGCGGCAGGCAGCGCCACGTAATTATATGCCAGCCCCAGCGTCAGCATCATACCTCCGCAATATGCGCCGACGGCGCTACCg  <  2:1066782/140‑1 (MQ=255)                                                                                                                                       |                                                                                                                                      GGCCTGCAAAATGTGCCAGAACCGCGTAGGGCGGATAAGGCGTTCACGCCGCATCCGGCAATAAGTAATGAGCACCGAGACTATAACCTACCCCAGTGGTTTCGCCAGCACCGGAGTATCCGCCGCTTGCTGGCGCTTATAGCGACCATACAGCAGCAACGACGACATCGCAGCAAACGAAAGCAGGGCGGCAGGCAGCGCCACGTAATTATATGCCAGCCCCAGCGTCAGCATCATACCTCCGCAATATGCGCCGACGGCGCTACCG  >  NC_000913/411210‑411477

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 24 ≤ ATCG/ATCG < 31 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑