Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	1,111,934	G→T	G358W (GGG→TGG)	opgH →	osmoregulated periplasmic glucans (OPGs) biosynthesis protein H

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,111,934	0	G	T	100.0%	162.1 / NA	46	G358W (GGG→TGG)	opgH	osmoregulated periplasmic glucans (OPGs) biosynthesis protein H
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base T (20/26);  total (20/26)

TTTCTATCGCCGCCGCCGTCGCCGCGTGAAGCGTAAAAGCGGTAATATCGATGACTTCTGCCGTCGCTGGGGCAGCCAGTACAGCTACATGGTGGTGCTGGATGCTGACTCGGTAATGACCGGTGATTGTTTGTGCGGGCTGGTGCGCCTGATGGAAGCCAACCCGAACGCCGGGATCATTCAGTCGTCGCCGAAAGCGTCCGGTATGGATACGCTGTATGCGCGCTGTCAGCAGTTCGCGACCCGCGTGTATGGGCCACTGTTTACAG  >  NC_000913/1111798‑1112066                                                                                                                                         |                                                                                                                                     tttCTATCGCCGCCGCCGTCGCCGCGTGAAGCGTAAAAGCGGTAATATCGATGACTTCTGCCGTCGCTGGGGCAGCCAGTACAGCTACATGGTGGTGCTGGATGCTGACTCGGTAATGACCGGTGATTGTTTGTGCTGGc                                                                                                                                   <  2:84438/140‑1 (MQ=255)                           tGAAGCGTAAAAGCGGTAATATCGATGACTTCTGCCGTCGCTGGAGCAGCCAGTACAGCTACATGGTGGTGCTGGATGCTGACTCGGTAATGACCGGTGATTGTTTGTGCTGGCTGGTGCGCCTGATGGAAGCCAACCCg                                                                                                         <  1:324168/140‑1 (MQ=255)                            gAAGCGTAAAAGCGGTAATATCGATGACTTCTGCCGTCGCTGGGGCAGCCAGTACAGCTACATGGTGGTGCTGGATGCTGACTCGGTAATGACCGGTGATTGTTTGTGCTGGCTGGTGCGCCTGATGGAAGCCAACCCGa                                                                                                        <  2:593780/140‑1 (MQ=255)                             aaGCGTAAAAGCGGTAATATCGATGACTTCTGCCGTCGCTGGGGCAGCCAGTACAGCTACATGGTGGTGCTGGATGCTGACTCGGTAATGACCGGTGATTGTTTGTGCTGGCTGGTGCGCCTGATGGAAGCCAACCCGaa                                                                                                       >  2:174668/1‑140 (MQ=255)                                cGTAAAAGCGGTAATATCGATGACTTCTGCCGTCGCTGGGGCAGCCAGTACAGCTACATGGTGGTGCTGGATGCTGACTCGGTAATGACCGGTGATTGTTTGTGCTGGCTGGTGCGCCTGATGGAAGCCAACCCGAACGc                                                                                                    <  1:9057/140‑1 (MQ=255)                                cGTAAAAGCGGTAATATCGATGACTTCTGCCGTCGCTGGGGCAGCCAGTACAGCTACATGGTGGTGCTGGATGCTGACTCGGTAATGACCGGTGATTGTTTGTGCTGGCTGGTGCGCCTGATGGAAGCCAACCCGAACGc                                                                                                    <  2:571371/140‑1 (MQ=255)                                cGTAAAAGCGGTAATATCGATGACTTCTGCCGTCGCTGGGGCAGCCAGTACAGCTACATGGTGGTGCTGGATGCTGACTCGGTAATGACCGGTGATTGTTTGTGCTGGCTGGTGCGCCTGATGGAAGCCAACCCGAACGc                                                                                                    <  2:607906/140‑1 (MQ=255)                                   aaaaGCGGTAATATCGATGACTTCTGCCGTCGCTGGGGCAGCCAGTACAGCTACATGGTGGTGCTGGATGCTGACTCGGTAATGACCGGTGATTGTTTGTGCTGGCTGGTGCGCCTGATGGAAGCCAACCCGAACGCCgg                                                                                                 <  2:38720/140‑1 (MQ=255)                                               atCGATGACTTCTGCCGTCGCTGGGGCAGCCAGTACAGCTACATGGTGGTGCTGGATGCTGACTCGGTAATGACCGGTGATTGTTTGTGCTGGCTGGTGCGCCTGATGGAAGCCAACCCGAACGCCGGGATCATTCAgtc                                                                                     <  2:1183408/140‑1 (MQ=255)                                               atCGATGACTTCTGCCGTCGCTGGGGCAGCCAGTACAGCTACATGGTGGTGCTGGATGCTGACTCGGTAATGACCGGTGATTGTTTGTGCTGGCTGGTGCGCCTGATGGAAGCCAACCCGAACGCCGGGATCATTCAgtc                                                                                     <  1:359646/140‑1 (MQ=255)                                               atCGATGACTTCTGCCGTCGCTGGGGCAGCCAGTACAGCTACATGGTGGTGCTGGATGCTGACTCGGTAATGACCGGTGATTGTTTGTGCTGGCTGGTGCGCCTGATGGAAGCCAACCCGAACGCCGGGATCATTCAgtc                                                                                     <  1:1228745/140‑1 (MQ=255)                                               atCGATGACTTCTGCCGTCGCTGGGGCAGCCAGTACAGCTACATGGTGGTGCTGGATGCTGACTCGGTAATGACCGGTGATTGTTTGTGCTGGCTGGTGCGCCTGATGGAAGCCAACCCGAACGCCGGGATCATTCAgtc                                                                                     >  2:673718/1‑140 (MQ=255)                                                   aTGACTTCTGCCGTCGCTGGGGCAGCCAGTACAGCTACATGGTGGTGCTGGATGCTGACTCGGTAATGACCGGAGATTGTTTGTGCTGGCTGGTGCGCCTGATGGAAGCCAACCCGAACGCCGGGATCATTCAGtcgtcg                                                                                 >  1:930708/1‑140 (MQ=255)                                                         tCTGCCGTCGCTGGGGCAGCCAGTACAGCTACATGGTGGTGCTGGATGCTGACTCGGTAATGACCGGTGATTGTTTGTGCTGGCTGGTGCGCCTGATGGAAGCCAACCCGAACGCCGGGATCATTCAGTCGTCGCCGaaa                                                                           >  2:101492/1‑140 (MQ=255)                                                             ccGTCGCTGGGGCAGCCAGTACAGCTACATGGTGGTGCTGGATGCTGACTCGGTAATGACCGGTGATTGTTTGTGCTGGCTGGTGCGCCTGATGGAAGCCAACCCGAACGCCGGGATCATTCAGTCGTCGCCGAAAGCGt                                                                       <  1:1135640/140‑1 (MQ=255)                                                              cGTCGCTGGGGCAGCCAGTACAGCTACATGGTGGTGCTGGATGCTGACTCGGTAATGTCCGGTGATTGTTTGTGCTGGCTGGTGCGCCTGATGGAAGCCAACCCGAACGCCGGGATCATTCAGTCGTCGCCGAAAGCGTc                                                                      <  1:220817/140‑1 (MQ=255)                                                              cGTCGCTGGGGCAGCCAGTACAGCTACATGGTGGTGCTGGATGCTGACTCGGTAATGACCGGTGATTGTTTGTGCTGGCTGGTGCGCCTGATGGAAGCCAACCCGAACGCCGGGATCATTCAGTCGTCGCCGAAAGCGTc                                                                      <  1:174668/140‑1 (MQ=255)                                                              cGTCGCTGGGGCAGCCAGTACAGCTACATGGTGGTGCTGGATGCTGACTCGGTAATGACCGGTGATTGTTTGTGCTGGCTGGTGCGCCTGATGGAAGCCAACCCGAACGCCGGGATCATTCAGTCGTCGCCGAAAGCGTc                                                                      <  1:1101397/140‑1 (MQ=255)                                                              cGTCGCTGGGGCAGCCAGTACAGCTACATGGTGGTGCTGGATGCTGACTCGGTAATGACCGGTGATTGTTTGTGCTGGCTGGTGCGCCTGATGGAAGCCAACCCGAACGCCGGGATCATTCAGTCGTCGCCGAAAGCGTc                                                                      <  2:176119/140‑1 (MQ=255)                                                                   cTGGGGCAGCCAGTACAGCTACATGGTGGTGCTGGATGCTGACTCGGTAATGACCGGTGATTGTTTGTGCTGGCTGGTGCGCCTGATGGAAGCCAACCCGAACGCCGGGATCATTCAGTCGTCGCCGAAAGCGTCCGGTa                                                                 <  1:101492/140‑1 (MQ=255)                                                                     ggggCAGCCAGTACAGCTACATGGTGGTGCTGGATGCTGACTCGGTAATGACCGGTGATTGTTTGTGCTGGCTGGTGCGCCTGATGGAAGCCAACCCGAACGCCGGGATCATTCAGTCGTCGCCGAAAGCGTCCGGTATg                                                               >  2:141333/1‑140 (MQ=255)                                                                         cAGCCAGTACAGCTACATGGTGGTGCTGGATGCTGACTCGGTAATGACCGGTGATTGTTTGTGCTGGCTGGTGCGCCTGATGGAAGCCAACCCGAACGCCGGGATCATTCAGTCGTCGCCGAAAGCGTCCGGTATGGATa                                                           <  1:344733/140‑1 (MQ=255)                                                                           gCCAGTACAGCTACATGGTGGTGCTGGATGCTGACTCGGTAATGACCGGTGATTGTTTGTGCTGGCTGGTGCGCCTGATGGAAGCCAACCCGAACGCCGGGATCATTCAGTCGTCGCCGAAAGCGTCCGGTATGGATACg                                                         >  1:504103/1‑140 (MQ=255)                                                                                tACAGCTACATGGTGGTGCTGGATGCTGACTCGGTAATGACCGGTGATTGTTTGTGCTGGCTGGTGCGCCTGATGGAAGCCAACCCGAACGCCGGGATCATTCAGTCGTCGCCGAAAGCGTCCgg                                                                   <  1:327663/125‑1 (MQ=255)                                                                                tACAGCTACATGGTGGTGCTGGATGCTGACTCGGTAATGACCGGTGATTGTTTGTGCTGGCTGGTGCGCCTGATGGAAGCCAACCCGAACGCCGGGATCATTCAGTCGTCGCCGAAAGCGTCCgg                                                                   >  2:327663/1‑125 (MQ=255)                                                                                tACAGCTACATGGTGGTGCTGGATGCTGACTCGGTAATGACCGGTGATTGTTTGTGCTGGCTGGTGCGCCTGATGGAAGCCAACCCGAACGCCGGGATCATTCAGTCGTCGCCGAAAGCGTCCGGTATGGATACGCTGTa                                                    >  2:1187576/1‑140 (MQ=255)                                                                                     cTACATGGTGGTGCTGGATGCTGACTCGGTAATGACCGGTGATTGTTTGTGCTGGCTGGTGCGCCTGATGGAAGCCAACCCGAACGCCGGGATCATTCAGTCGTCGCCGAAAGCGTCCGGTATGGATACGCTGTATgcgc                                               >  2:682319/1‑140 (MQ=255)                                                                                      tACATGGTGGTGCTGGATGCTGACTCGGTAATGACCGGTGATTGTTTGTGCTGGCTGGTGCGCCTGATGGAAGCCAACCCGAACGCCGGGATCATTCAGTCGTCGCCGAAAGCGTCCGGTATGGATACGCTGTATgcgcg                                              >  2:1137869/1‑140 (MQ=255)                                                                                        cATGGTGGTGCTGGATGCTGACTCGGTAATGACCGGTGATTGTTTGTGCTGGCTGGTGCGCCTGATGGAAGCCAACCCGAACGCCGGGATCATTCAGTCGTCGCCGAAAGCGTCCGGTATGGATACGCTGTATGCGCGCt                                            <  2:930708/140‑1 (MQ=255)                                                                                          tggtggTGCTGGATGCTGACTCGGTAATGACCGGTGATTGTTTGTGCTGGCTGGTGCGCCTGATGGAAGCCAACCCGAACGCCGGGATCATTCAGTCGTCGCCGAAAGCGTCCGGTATGGATACGCTGTATGCGCGCTGt                                          >  2:1154358/1‑140 (MQ=255)                                                                                          tggtggTGCTGGATGCTGACTCGGTAATGACCGGTGATTGTTTGTGCTGGCTGGTGCGCCTGATGGAAGCCAACCCGAACGCCGGGATCATTCAGTCGTCGCCGAAAGCGTCCGGTATGGATACGCTGTATGCGCGCTGt                                          >  2:1011371/1‑140 (MQ=255)                                                                                               gtgCTGGATGCTGACTCGGTAATGACCGGTGATTGTTTGTGCTGGCTGGTGCGCCTGATGGAAGCCAACCCGAACGCCGGGATCATTCAGTCGTCGCCGAAAGCGTCCGGTATGGATACGCTGTATGCGCGCTGTcagca                                     >  1:408442/1‑140 (MQ=255)                                                                                                tgCTGGATGCTGACTCGGTAATGACCGGTGATTGTTTGTGCTGGCTGGTGCGCCTGATGGAAGCCAACCCGAACGCCGGGATCATTCAGTCGTCGCCGAAAGCGTCCGGTATGGATACACTGTATGCGCGCTGTcagcag                                    >  2:1139556/1‑140 (MQ=255)                                                                                                  cTGGATGCTGACTCGGTAATGACCGGTGATTGTTTGTGCTGGCTGGTGCGCCTGATGGAAGCCAACCCGAACGCCGGGATCATTCAGTCGTCGCCGAAAGCGTCCGGTATGGATACGCTGTATGCGCGCTGTCAGCAGtt                                  <  2:1207020/140‑1 (MQ=255)                                                                                                  cTGGATGCTGACTCGGTAATGACCGGTGATTGTTTGTGCTGGCTGGTGCGCCTGATGGAAGCCAACCCGAACGCCGGGATCATTCAGTCGTCGCCGAAAGCGTCCGGTATGGATACGCTGTATGCGCGCTGTCAGCAGtt                                  >  1:786940/1‑140 (MQ=255)                                                                                                       tGCTGACTCGGTAATGACCGGTGATTGTTTGTGCTGGCTGGTGCGCCTGATGGAAGCCAACCCGAACGCCGGGATCATTCAGTCGTCGCCGAAAGCGTCCGGTATGGATACGCTGTATGCGCGCTGTCAGCAGTTCGCGa                             >  1:1142851/1‑140 (MQ=255)                                                                                                         cTGACTCGGTAATGACCGGTGATTGTTTGTGCTGGCTGGTGCGCCTGATGGAAGCCAACCCGAACGCCGGGATCATTCAGTCGTCGCCGAAAGCGTCCGGTATGGATACGCTGTATGCGCGCTGTCAGCAGTTCGCGAcc                           <  2:612923/140‑1 (MQ=255)                                                                                                                ggTAATGACCGGTGATTGTTTGTGCTGGCTGGTGCGCCTGATGGAAGCCAACCCGAACGCCGGGATCATTCAGTCGTCGCCGAAAGCGTCCGGTATGGATACGCTGTATGCGCGCTGTCAGCAGTTCGCGACCCGCgtgt                    <  2:17630/140‑1 (MQ=255)                                                                                                                 gTAATGACCGGTGATTGTTTGTGCTGGCTGGTGCGCCTGATGGAAGCCAACCCGAACGCCGGGATCATTCAGTCGTCGCCGAAAGCGTCCGGTATGGATACGCTGTATGCGCGCTGTCAGCAGTTCGCGACCCGCGTGTa                   >  2:1100429/1‑140 (MQ=255)                                                                                                                       aCCGGTGATTGTTTGTGCTGGCTGGTGCGCCTGATGGAAGCCAACCCGAACGCCGGGATCATTCAGTCGTCGCCGAAAGCGTCCGGTATGGATACGCTGTATGCGCGCTGTCAGCAGTTCGCGACCCGCGTGTATGGGcc             >  2:532394/1‑140 (MQ=255)                                                                                                                        ccGGTGATTGTTTGTGCTGGCTGGTGCGCCTGATGGAAGCCAACCCGAACGCCGGGATCATTCAGTCGTCGCCGAAAGCGTCCGGTATGGATACGCTGTATGCGCGCTGTCAGCAGTTCGCGACCCGCGTGTATGGGCCa            <  2:504103/140‑1 (MQ=255)                                                                                                                        ccGGTGATTGTTTGTGCTGGCTGGTGCGCCTGATGGAAGCCAACCCGAACGCCGGGATCATTCAGTCGTCGCCGAAAGCGTCCGGTATGGATACACTGTATGCGCGCTGTCAGCAGTTCGCGACCCGCGTGTATGGGCCa            <  1:1139556/140‑1 (MQ=255)                                                                                                                           gTGATTGTTTGTGCTGGCTGGTGCGCCTGATGGAAGCCAACCCGAACGCCGGGATCATTCAGTCGTCGCCGAAAGCGTCCGGTATGGATACGCTGTATGCGCGCTGTCAGCAGTTCGCGACCCGCGTGTATGGGCCACTg         >  1:747968/1‑140 (MQ=255)                                                                                                                            tGATTGTTTGTGCTGGCTGGTGCGCCTGATGGAAGCCACCCCGAACGCCGGGATCATTCAGTCGTCGCCGAAAGCGTCCGGTATGGATACGCTGTATGCGCGCTGTCAGCAGTTCGCGACCCGCGTGTATGGGCCa            >  1:1138464/1‑136 (MQ=255)                                                                                                                            tGATTGTTTGTGCTGGCTGGTGCGCCTGATGGAAGCCACCCCGAACGCCGGGATCATTCAGTCGTCGCCGAAAGCGTCCGGTATGGATACGCTGTATGCGCGCTGTCAGCAGTTCGCGACCCGCGTGTATGGGCCa            <  2:1138464/136‑1 (MQ=255)                                                                                                                                  tttgtGCTGGCTGGTGCGCCTGATGGAAGCCAACCCGAACGCCGGGATCATTCAGTCGTCGCCGAAAGCGTCCGGTATGGATACGCTGTATGCGCGCTGTCAGCAGTTCGCGACCCGCGTGTATGGGCCACTGTTTACAg  <  1:1011371/140‑1 (MQ=255)                                                                                                                                         |                                                                                                                                     TTTCTATCGCCGCCGCCGTCGCCGCGTGAAGCGTAAAAGCGGTAATATCGATGACTTCTGCCGTCGCTGGGGCAGCCAGTACAGCTACATGGTGGTGCTGGATGCTGACTCGGTAATGACCGGTGATTGTTTGTGCGGGCTGGTGCGCCTGATGGAAGCCAACCCGAACGCCGGGATCATTCAGTCGTCGCCGAAAGCGTCCGGTATGGATACGCTGTATGCGCGCTGTCAGCAGTTCGCGACCCGCGTGTATGGGCCACTGTTTACAG  >  NC_000913/1111798‑1112066

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 23 ≤ ATCG/ATCG < 30 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑