breseq  version 0.35.4  revision f352f80f4bc9
mutation predictions | marginal predictions | summary statistics | genome diff | command line log

Predicted mutations
evidence position mutation annotation gene description
RA 102,072 T→A D436E (GAT→GAA murC → UDP‑N‑acetylmuramate‑‑L‑alanine ligase
MC JC 257,908 Δ776 bp insB9[crl] insB9, insA9, [crl]
RA 697,054 (A)7→8 intergenic (‑7/+3) leuW ← / ← metT tRNA‑Leu/tRNA‑Met
RA 889,923 A→G intergenic (‑104/‑166) ybjL ← / → ybjM putative transport protein YbjL/putative inner membrane protein
RA 1,196,220 C→T H366H (CAC→CAT icd → isocitrate dehydrogenase
RA 1,196,232 C→T T370T (ACC→ACT icd → isocitrate dehydrogenase
RA 1,196,245 T→C L375M (TTA→CTG)  icd → isocitrate dehydrogenase
RA 1,196,247 A→G L375M (TTA→CTG icd → isocitrate dehydrogenase
RA 1,212,088 (C)8→7 intergenic (‑85/+172) iraM ← / ← ymgK anti‑adaptor protein IraM, inhibitor of sigma(S) proteolysis/protein YmgK
RA 1,632,837 A→G intergenic (‑552/‑53) ydfJ ← / → ynfT putative transporter YdfJ/Qin prophage; protein YnfT
JC JC 1,879,829 Δ1 bp :: IS186 (–) +6 bp :: Δ1 bp coding (115‑120/360 nt) yeaR ← DUF1971 domain‑containing protein YeaR
MC JC 1,978,503 Δ776 bp insB‑5insA‑5 insB‑5, insA‑5
RA 2,173,363 Δ2 bp pseudogene (915‑916/1358 nt) gatC ← galactitol‑specific PTS enzyme IIC component
RA 2,874,997 A→G R141R (CGT→CGC cysN ← sulfate adenylyltransferase subunit 1
RA 2,933,881 A→G intergenic (‑193/‑354) fucA ← / → fucP L‑fuculose‑phosphate aldolase/L‑fucose:H(+) symporter
RA 3,248,470 T→C L11P (CTA→CCA)  mzrA → modulator protein MzrA
RA 3,278,186 G→C R160R (CGC→CGG agaR ← DNA‑binding transcriptional repressor AgaR
RA 3,354,487 C→T intergenic (‑437/‑238) yhcC ← / → gltB radical SAM family oxidoreductase YhcC/glutamate synthase subunit GltB
RA 3,560,455 +G pseudogene (151/758 nt) glpR ← DNA‑binding transcriptional repressor GlpR
RA 3,632,717 A→G intergenic (‑127/+135) yhiJ ← / ← yhiL DUF4049 domain‑containing protein YhiJ/putative uncharacterized protein YhiL
RA 3,693,550 C→A A563S (GCC→TCC)  bcsA ← cellulose synthase catalytic subunit
RA 3,815,883 +C coding (667/687 nt) rph ← truncated RNase PH
RA 4,296,381 +GC intergenic (+587/+55) gltP → / ← yjcO glutamate/aspartate : H(+) symporter GltP/Sel1 repeat‑containing protein YjcO
RA 4,325,847 2 bp→CA intergenic (‑106/+551) yjdN ← / ← yjdM conserved protein YjdN/conserved protein YjdM

Unassigned missing coverage evidence
   seq id start end size ←reads reads→ gene description
* * ÷ NC_000913 226713–226968 228563–226989 22–1851 27 [25] [25] 31 rrlH 23S ribosomal RNA
* * ÷ NC_000913 1196275 1211431 15157 29 [25] [24] 27 [icd]–[icdC] [icd],ymfD,ymfE,lit,intE,xisE,ymfH,ymfI,ymfJ,ymfK,ymfT,ymfL,ymfM,oweE,ymfN,aaaE,ymfR,beeE,jayE,ymfQ,ycfK,tfaP,tfaE,stfE,pinE,mcrA,[icdC]
* * ÷ NC_000913 3423766–3424234 3424528–3424239 6–763 27 [25] [26] 28 [rrfD]–[rrlD] [rrfD],[rrlD]

Unassigned new junction evidence
  seq id position reads (cov) reads (cov) score skew freq annotation gene product
* ? NC_000913 = 12994980 (0.000)114 (1.030) 72/266 0.2 100% intergenic (+253/‑33) ychE/insH21 putative inner membrane protein/IS5 transposase and trans‑activator
?NC_000913 1300698 = 0 (0.000)intergenic (+151/‑484) insH21/oppA IS5 transposase and trans‑activator/oligopeptide ABC transporter periplasmic binding protein
* ? NC_000913 3363001 =172 (1.510)179 (1.580) 79/272 0.1 67.5% coding (195/2382 nt) yhcD putative fimbrial usher protein YhcD
?NC_000913 3766087 = 2 (0.020)coding (3905/4134 nt) rhsA rhs element protein RhsA
* ? NC_000913 = 3653230NA (NA)109 (0.950) 67/274 0.3 96.5% noncoding (1/1195 nt) IS5 repeat region
?NC_000913 = 3766089 4 (0.040)coding (3907/4134 nt) rhsA rhs element protein RhsA