breseq  version 0.35.4  revision f352f80f4bc9
mutation predictions | marginal predictions | summary statistics | genome diff | command line log

Predicted mutations
evidence position mutation annotation gene description
MC JC 257,908 Δ776 bp insB9[crl] insB9, insA9, [crl]
RA 661,803 T→A D159V (GAT→GTT)  lipB ← lipoyl(octanoyl) transferase
RA 889,923 A→G intergenic (‑104/‑166) ybjL ← / → ybjM putative transport protein YbjL/putative inner membrane protein
JC JC 949,972 IS1 (–) +9 bp coding (305‑313/591 nt) ycaK → putative NAD(P)H‑dependent oxidoreductase YcaK
RA 1,196,220 C→T H366H (CAC→CAT icd → isocitrate dehydrogenase
RA 1,196,232 C→T T370T (ACC→ACT icd → isocitrate dehydrogenase
RA 1,196,245 T→C L375M (TTA→CTG)  icd → isocitrate dehydrogenase
RA 1,196,247 A→G L375M (TTA→CTG icd → isocitrate dehydrogenase
JC JC 1,879,829 Δ1 bp :: IS186 (–) +6 bp :: Δ1 bp coding (115‑120/360 nt) yeaR ← DUF1971 domain‑containing protein YeaR
RA 1,912,629 T→C intergenic (‑53/+139) htpX ← / ← prc zinc dependent endoprotease/tail‑specific protease
MC JC 1,978,503 Δ776 bp insB‑5insA‑5 insB‑5, insA‑5
RA 2,173,363 Δ2 bp pseudogene (915‑916/1358 nt) gatC ← galactitol‑specific PTS enzyme IIC component
RA 3,354,487 C→T intergenic (‑437/‑238) yhcC ← / → gltB radical SAM family oxidoreductase YhcC/glutamate synthase subunit GltB
RA 3,560,455 +G pseudogene (151/758 nt) glpR ← DNA‑binding transcriptional repressor GlpR
RA 3,772,854 G→A A192T (GCC→ACC)  mtlA → mannitol‑specific PTS enzyme IICBA component
RA 3,809,221 T→A W228R (TGG→AGG)  waaA → KDO transferase
RA 3,815,883 +C coding (667/687 nt) rph ← truncated RNase PH
RA 4,296,381 +GC intergenic (+587/+55) gltP → / ← yjcO glutamate/aspartate : H(+) symporter GltP/Sel1 repeat‑containing protein YjcO
RA 4,526,837 G→T Q18K (CAA→AAA)  sgcR ← KpLE2 phage‑like element; putative DNA‑binding transcriptional regulator SgcR
RA 4,565,136 G→A R239C (CGT→TGT)  yjiM ← putative dehydratase subunit

Unassigned missing coverage evidence
   seq id start end size ←reads reads→ gene description
* * ÷ NC_000913 696716 696862 147 29 [25] [0] 58 [metU]–glnW [metU],glnW
* * ÷ NC_000913 1196255 1211438 15184 36 [22] [25] 27 [icd]–[icdC] [icd],ymfD,ymfE,lit,intE,xisE,ymfH,ymfI,ymfJ,ymfK,ymfT,ymfL,ymfM,oweE,ymfN,aaaE,ymfR,beeE,jayE,ymfQ,ycfK,tfaP,tfaE,stfE,pinE,mcrA,[icdC]
* * ÷ NC_000913 3423755–3424234 3424526–3424238 5–772 28 [23] [26] 28 [rrfD]–[rrlD] [rrfD],[rrlD]

Unassigned new junction evidence
  seq id position reads (cov) reads (cov) score skew freq annotation gene product
* ? NC_000913 696863 =0 (0.000)47 (0.570)
+GAAGAAACAATC
22/250 2.5 46.8% intergenic (‑33/+2) glnW/glnU tRNA‑Gln/tRNA‑Gln
?NC_000913 = 697134 117 (1.300)intergenic (‑1/+379) metT/asnB tRNA‑Met/asparagine synthetase B
* ? NC_000913 = 12994980 (0.000)94 (1.080) 61/266 0.2 100% intergenic (+253/‑33) ychE/insH21 putative inner membrane protein/IS5 transposase and trans‑activator
?NC_000913 1300698 = 0 (0.000)intergenic (+151/‑484) insH21/oppA IS5 transposase and trans‑activator/oligopeptide ABC transporter periplasmic binding protein
* ? NC_000913 3363001 =150 (1.670)142 (1.590) 70/272 0.1 65.1% coding (195/2382 nt) yhcD putative fimbrial usher protein YhcD
?NC_000913 3766087 = 3 (0.030)coding (3905/4134 nt) rhsA rhs element protein RhsA
* ? NC_000913 = 3653230NA (NA)100 (1.110) 52/274 0.4 94.3% noncoding (1/1195 nt) IS5 repeat region
?NC_000913 = 3766089 6 (0.070)coding (3907/4134 nt) rhsA rhs element protein RhsA