Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	2,318,816	T→C	E354G (GAA→GGA)	rcsC ←	sensory histidine kinase RcsC

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	2,318,816	0	T	C	100.0%	267.7 / NA	69	E354G (GAA→GGA)	rcsC	sensory histidine kinase RcsC
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base C (32/37);  total (32/37)

GGCGGTCCTCATGCGTAAGCATATTGAGATAGGTATGCGCCAGTTCGTTACTTAAAATATTGACGCCATCAGCGGTACGCAAAATGCAGATACCCACTGGCGCGGAGGCGACAATCTTGCGATTGAACTGCTCATGTTCTTCCAGTCGCAGGGCGTCGCTTTCCGCCGGAATGAAAATACGTCGCTCGTACATCCGTGCGAGAGTAAACAATGCAGCTCCGGCAAGCACATTCAGCAAAATTGCGTTAAGGATCAACATGCGAATGCGTTCCA  >  NC_000913/2318679‑2318951                                                                                                                                          |                                                                                                                                        ggCGGTCCTCATGCGTAAGCATATTGAGATAGGTATGCGCCAGTTCGTTACTTAAAATATTGACGCCATCAGCGGTACGCAAAATGCAGATACCCACTGGCGCGGAGGCGACAATCTTGCGATTGAACTGCTCATGtcct                                                                                                                                       >  2:329166/1‑140 (MQ=255)              cGTAAGCATATTGAGATAGGTATGCGCCAGTTCGTTACTTAAAATATTGACGCCATCAGCGGTACGCAAAATGCAGATACCCACTGGCGCGGAGGCGACAATCTTGCGATTGAACTGCTCATGTCCTTCCAGTCGCAggg                                                                                                                          <  1:1383803/140‑1 (MQ=255)                  aGCATATTGAGATAGGTATGCGCCAGTTCGTTACTTAAAATATTGACGCCATCAGCGGTACGCAAAATGCAGATACCCACTGGCGCGGAGGCGACAATCTTGCGATTGAACTGCTCATGTCCTTCCAGTCGCAGGGCGTc                                                                                                                      >  1:41793/1‑140 (MQ=255)                   gCATATTGAGATAGGTATGCGCCAGTTCGTTACTTAAAATATTGACGCCATCAGCGGTACGCAAAATGCAGATACCCACTGGCGCGGAGGCGACAATCTTGCGATTGAACTGCTCATGTCCTTCCAGTCGCAGGGCGTCg                                                                                                                     >  1:1232080/1‑140 (MQ=255)                       atTGAGATAGGTATGCGCCAGTTCGTTACTTAAAATATTGACGCCATCAGCGGTACGCAAAATGCAGATACCCACTGGCGCGGAGGCGACAATCTTGCGATTGAACTGCTCATGTCCTTCCAGTCGCAGGGCGTCGCttt                                                                                                                 >  2:993956/1‑140 (MQ=255)                           agaTAGGTATGCGCCAGTTCGTTACTTAAAATATTGACGCCATCAGCGGTACGCAAAATGCAGATACCCACTGGCGCGGAGGCGACAATCTTGCGATTGAACTGCTCATGTCCTTCCAGTCGCAGGGCGTCGCTTTccgc                                                                                                             <  1:1102509/140‑1 (MQ=255)                           agaTAGGTATGCGCCAGTTCGTTACTTAAAATATTGACGCCATCAGCGGTACGCAAAATGCAGATACCCACTGGCGCGGAGGCGACAATCTTGCGATTGAACTGCTCATGTCCTTCCAGTCGCAGGGCGTCGCTTTccgc                                                                                                             <  2:307603/140‑1 (MQ=255)                           agaTAGGTATGCGCCAGTTCGTTACTTAAAATATTGACGCCATCAGCGGTACGCAAAATGCAGATACCCACTGGCGCGGAGGCGACAATCTTGCGATTGAACTGCTCATGTCCTTCCAGTCGCAGGGCGTCGCTTTccgc                                                                                                             >  1:435732/1‑140 (MQ=255)                             aTAGGTATGCGCCAGTTCGTTACTTAAAATATTGACGCCATCAGCGGTACGCAAAATGCAGATACCCACTGGCGCGGAGGCGACAATCTTGCGATTGAACTGCTCATGTCCTTCCAGTCGCAGGGCGTCGCTTTccgccg                                                                                                           >  1:704398/1‑140 (MQ=255)                             aTAGGTATGCGCCAGTTCGTTACTTAAAATATTGACGCCATCAGCGGTACGCAAAATGCAGATACCCACTGGCGCGGAGGCGACAATCTTGCGATTGAACTGCTCATGTCCTTCCAGTCGCAGGGCGTCGCTTTccgc                                                                                                             >  2:811092/1‑138 (MQ=255)                             aTAGGTATGCGCCAGTTCGTTACTTAAAATATTGACGCCATCAGCGGTACGCAAAATGCAGATACCCACTGGCGCGGAGGCGACAATCTTGCGATTGAACTGCTCATGTCCTTCCAGTCGCAGGGCGTCGCTTTccgc                                                                                                             <  1:811092/138‑1 (MQ=255)                             aTAGGTATGCGCCAGTTCGTTACTTAAAATATTGACGCCATCAGCGGTACGCAAAATGCAGATACCCACTGGCGCGGAGGCGACAATCTTGCGATTGAACTGCTCATGTCCTTCCAGTCGCAGGGCGTCGCTTTccgc                                                                                                             <  2:456434/138‑1 (MQ=255)                             aTAGGTATGCGCCAGTTCGTTACTTAAAATATTGACGCCATCAGCGGTACGCAAAATGCAGATACCCACTGGCGCGGAGGCGACAATCTTGCGATTGAACTGCTCATGTCCTTCCAGTCGCAGGGCGTCGCTTTccgc                                                                                                             >  1:456434/1‑138 (MQ=255)                                       gcCAGTTCGTTACTTAAAATATTGACGCCATCAGCGGTACGCAAAATGCAGATACCCACTGGCGCGGAGGCGACAATCTTGCGATTGAACTGCTCATGTCCTTCCAGTCGCAGGGCGTCGCTTTCCGCCGGAATGAAAAt                                                                                                 >  2:1207744/1‑140 (MQ=255)                                       gcCAGTTCGTTACTTAAAATATTGACGCCATCAGCGGTACGCAAAATGCAGATACCCACTGGCGCGGAGGCGACAATCTTGCGATTGAACTGCTCATGTCCTTCCAGTCGCAGGGCGTCGCTTTCCGCCGGAATGAAAAt                                                                                                 >  1:572507/1‑140 (MQ=255)                                        ccAGTTCGTTACTTAAAATATTGACGCCATCAGCGGTACGCAAAATGCAGATACCCACTGGCGCGGAGGCGACAATCTTGCGATTGAACTGCTCATGTCCTTCCAGTCGCAGGGCGTCGCTTTCCGCCGGAATGAAAATa                                                                                                <  2:572507/140‑1 (MQ=255)                                          aGTTCGTTACTTAAAATATTGACGCCATCAGCGGTACGCAAAATGCAGATACCCACTGGCGCGGAGGCGACAATCTTGCGATTGAACTGCTCATGTCCTTCCAGTCGCAGGGCGTCGCTTTCCGCCGGAATGAAAATACg                                                                                              <  2:663786/140‑1 (MQ=255)                                          aGTTCGTTACTTAAAATATTGACGCCATCAGCGGTACGCAAAATGCAGATACCCACTGGCGCGGAGGCGACAATCTTGCGATTGAACTGCTCATGTCCTTCCAGTCGCAGGGCGTCGCTTTCCGCCGGAATGAAAATACg                                                                                              <  1:143495/140‑1 (MQ=255)                                           gTTCGTTACTTAAAATATTGACGCCATCAGCGGTACGCAAAATGCAGATACCCACTGGCGCGGAGGCGACAATCTTGCGATTGAACTGCTCATGTCCTTCCAGTCGCAGGGCGTCGCTTTCCGCCGGaa                                                                                                        >  2:321714/1‑129 (MQ=255)                                           gTTCGTTACTTAAAATATTGACGCCATCAGCGGTACGCAAAATGCAGATACCCACTGGCGCGGAGGCGACAATCTTGCGATTGAACTGCTCATGTCCTTCCAGTCGCAGGGCGTCGCTTTCCGCCGGaa                                                                                                        <  1:321714/129‑1 (MQ=255)                                           gTTCGTTACTTAAAATATTGACGCCATCAGCGGTACGCAAAATGCAGATACCCACTGGCGCGGAGGCGACAATCTTGCGATTGAACTGCTCATGTCCTTCCAGTCGCAGGGCGTCGCTTTCCGCCGGAATGAAAATACGt                                                                                             <  2:41793/140‑1 (MQ=255)                                           gTTCGTTACTTAAAATATTGACGCCATCAGCGGTACGCAAAATGCAGATACCCACTGGCGCGGAGGCGACAATCTTGCGATTGAACTGCTCATGTCCTTCCAGTCGCAGGGCGTCGCTTTCCGCCGGAATGAAAATACGt                                                                                             >  2:1145291/1‑140 (MQ=255)                                            ttCGTTACTTAAAATATTGACGCCATCAGCGGTACGCAAAATGCAGATACCCACTGGCGCGGAGGCGACAATCTTGCGATTGAACTGCTCATGTCCTTCCAGTCGCAGGGCGTCGCTTTCCGCCGGAATGAAAATACGTc                                                                                            >  1:89861/1‑140 (MQ=255)                                            ttCGTTACTTAAAATATTGACGCCATCAGCGGTACGCAAAATGCAGATACCCACTGGCGCGGAGGCGACAATCTTGCGATTGAACTGCTCATGTCCTTCCAGTCGCAGGGCGTCGCTTTCCGCCGGAATGAAAATACGTc                                                                                            <  1:1207744/140‑1 (MQ=255)                                            ttCGTTACTTAAAATATTGACGCCATCAGCGGTACGCAAAATGCAGATACCCACTGGCGCGGAGGCGACAATCTTGCGATTGAACTGCTCATGTCCTTCCAGTCGCAGGGCGTCGCTTTCCGCCGGAATGAAAATACGTc                                                                                            >  1:526864/1‑140 (MQ=255)                                              cGTTACTTAAAATATTGACGCCATCAGCGGTACGCAAAATGCAGATACCCACTGGCGCGGAGGCGACAATCTTGCGATTGAACTGCTCATGTCCTTCCAGTCGCAGGGCGTCGCTTTCCGCCGGAATGAAAATACGTCGc                                                                                          <  1:596922/140‑1 (MQ=255)                                                  aCTTAAAATATTGACGCCATCAGCGGTACGCAAAATGCAGATACCCACTGGCGCGGAGGCGACAATCTTGCGATTGAACTGCTCATGTCCTTCCAGTCGCAGGGCGTCGCTTTCCGCCGGAATGAAAATATGTCGCTCGt                                                                                      <  2:1232080/140‑1 (MQ=255)                                                      aaaaTATTGACGCCATCAGCGGTACGCAAAATGCAGATACCCACTGGCGCGGAGGCGACAATCTTGCGATTGAACTGCTCATGTCCTTCCAGTCGCAGGGCGTCGCTTTCCGCCGGAATGAAAATACGTCGCTCGTACAt                                                                                  >  2:867619/1‑140 (MQ=255)                                                       aaaTATTGACGCCATCAGCGGTACGCAAAATGCAGATACCCACTGGCGCGGAGGCGACAATCTTGCGATTGAACTGCTCATGTCCTTCCAGTCGCAGGGCGTCGCTTTCCGCCGGAATGAAAATACGTCGCTCGTACATc                                                                                 <  2:435732/140‑1 (MQ=255)                                                           atTGACGCCATCAGCGGTACGCAAAATGCAGATACCCACTGGCGCGGAGGCGACAATCTTGCGATTGAACTGCTCATGTCCTTCCAGTCGCAGGGCGTCGCTTTCCGCCGGAATGAAAATACGTCGCTCGTACATCCGTg                                                                             <  2:526864/140‑1 (MQ=255)                                                           atTGACGCCATCAGCGGTACGCAAAATGCAGATACCCACTGGCGCGGAGGCGACAATCTTGCGATTGAACTGCTCATGTCCTTCCAGTCGCAGGGCGTCGCTTTCCGCCGGAATGAAAATACGTCGCTCGTACATCCGTg                                                                             <  1:127771/140‑1 (MQ=255)                                                             tGACGCCATCAGCGGTACGCAAAATGCAGATACCCACTGGCGCGGAGGCGACAATCTTGCGATTGAACTGCTCATGTCCTTCCAGTCGCAGGGCGTCGCTTTCCGCCGGAATGAAAATACGTCGCTCGTACATCCGTGCg                                                                           >  2:284527/1‑140 (MQ=255)                                                             tGACGCCATCAGCGGTACGCAAAATGCAGATACCCACTGGCGCGGAGGCGACAATCTTGCGATTGAACTGCTCATGTCCTTCCAGTCGCAGGGCGTCGCTTTCCGCCGGAATGAAAATACGTCGCTCGTACATCCGTGCg                                                                           >  1:456477/1‑140 (MQ=255)                                                             tGACGCCATCAGCGGTACGCAAAATGCAGATACCCACTGGCGCGGAGGCGACAATCTTGCGATTGAACTGCTCATGTCCTTCCAGTCGCAGGGCGTCGCTTTCCGCCGGAATGAAAATACGTCGCTCGTACATCCGTGCg                                                                           <  1:993956/140‑1 (MQ=255)                                                             tGACGCCATCAGCGGTACGCAAAATGCAGATACCCACTGGCGCGGAGGCGACAATCTTGCGATTGAACTGCTCATGTCCTTCCAGTCGCAGGGCGTCGCTTTCCGCCGGAATGAAAATACGTCGCTCGTACATCCGTGCg                                                                           <  2:442746/140‑1 (MQ=255)                                                               aCGCCATCAGCGGTACGCAAAATGCAGATACCCACTGGCGCGGAGGCGACAATCTTGCGATTGAACTGCTCATGTCCTTCCAGTCGCAGGGCGTCGCTTTCCGCCGGAATGAAAATACGTCGCTCGTACATCCGTGCgag                                                                         <  2:5737/140‑1 (MQ=255)                                                                cGCCATCAGCGGTACGCAAAATGCAGATACCCACTGGCGCGGAGGCGACAATCTTGCGATTGAACTGCTCATGTCCTTCCAGTCGCAGGGCGTCGCTTTCCGCCGGAATGAAAATACGTCGCTCGTACATCCGTGCgaga                                                                        <  1:284527/140‑1 (MQ=255)                                                                cGCCATCAGCGGTACGCAAAATGCAGATACCCACTGGCGCGGAGGCGACAATCTTGCGATTGAACTGCTCATGTCCTTCCAGTCGCAGGGCGTCGCTTTCCGCCGGAATGAAAATACGTCGCTCGTACATCCGTGCgaga                                                                        <  1:329166/140‑1 (MQ=255)                                                                    aTCAGCGGTACGCAAAATGCAGATACCCACTGGCGCGGAGGCGACAATCTTGCGATTGAACTGCTCATGTCCTTCCAGTCGCAGGGCGTCGCTTTCCGCCGGAATGAAAATACGTCGCTCGTACATCCGTGCGAGAGTaa                                                                    >  1:757507/1‑140 (MQ=255)                                                                          ggTACGCAAAATGCAGATACCCACTGGCGCGGAGGCGACAATCTTGCGATTGAACTGCTCATGTCCTTCCAGTCGCAGGGCGTCGCTTTCCGCCGGAATGAAAATACGTCGCTCGTACATCCGTGCGAGAGTAAACAATg                                                              <  2:534979/140‑1 (MQ=255)                                                                          ggTACGCAAAATGCAGATACCCACTGGCGCGGAGGCGACAATCTTGCGATTGAACTGCTCATGTCCTTCCAGTCGCAGGGCGTCGCTTTCCGCCGGAATGAAAATACGTCGCTCGTACATCCGTGCGAGAGTAAACAATg                                                              <  1:107468/140‑1 (MQ=255)                                                                            tACGCAAAATGCAGATACCCACTGGCGCGGAGGCGACAATCTTGCGATTGAACTGCTCATGTCCTTCCAGTCGCAGGGCGTCGCTTTCCGCCGGAATGAAAATACGTCGCTCGTACATCCGTg                                                                             >  1:1280956/1‑123 (MQ=255)                                                                            tACGCAAAATGCAGATACCCACTGGCGCGGAGGCGACAATCTTGCGATTGAACTGCTCATGTCCTTCCAGTCGCAGGGCGTCGCTTTCCGCCGGAATGAAAATACGTCGCTCGTACATCCGTg                                                                             <  2:1280956/123‑1 (MQ=255)                                                                            tACGCAAAATGCAGATACCCACTGGCGCGGAGGCGACAATCTTGCGATTGAACTGCTCATGTCCTTCCAGTCGCAGGGCGTCGCTTTCCGCCGGAATGAAAATACGTCGCTCGTACATCCGTGCGAGAGTAAACAATGCa                                                            <  1:1145291/140‑1 (MQ=255)                                                                             aCGCAAAATGCAGATACCCACTGGCGCGGAGGCGACAATCTTGCGATTGAACTGCTCATGTCCTTCCAGTCGCAGGGCGTCGCTTTCCGCCGGAATGAAAATACGTCGCTCGTACATCCGTGCGAGAGTAAACAATGCAg                                                           >  1:783147/1‑140 (MQ=255)                                                                              cGCAAAATGCAGATACCCACTGGCGCGGAGGCGACAATCTTGCGATTGAACTGCTCATGTCCTTCCAGTCGCAGGGCGTCGCTTTCCGCCGGAATGAAAATACGTCGCTCGTACATCCGTGCGAGAGTAAACAATGCAGc                                                          >  2:732881/1‑140 (MQ=255)                                                                              cGCAAAATGCAGATACCCACTGGCGCGGAGGCGACAATCTTGCGATTGAACTGCTCATGTCCTTCCAGTCGCAGGGCGTCGCTTTCCGCCGGAATGAAAATACGTCGCTCGTACATCCGTGCGAGAGTAAACAATGCAGc                                                          >  2:311143/1‑140 (MQ=255)                                                                                 aaaaTGCAGATACCCACTGGCGCGGAGGCGACAATCTTGCGATTGAACTGCTCATGTCCTTCCAGTCGCAGGGCGTCGCTTTCCGCCGGAATGAAAATACGTCGCTCGTACATCCGTGCGAGAGTAAACAATGCAGCTcc                                                       <  2:89861/140‑1 (MQ=255)                                                                                   aaTGCAGATACCCACTGGCGCGGAGGCGACAATCTTGCGATTGAACTGCTCATGTCCTTCCAGTCGCAGGGCGTCGCTTTCCGCCGGAATGAAAATACGTCGCTCGTACATCCGTGCGAGAGTAAACAATGCAGCTCCgg                                                     <  1:154260/140‑1 (MQ=255)                                                                                          aTACCCACTGGCGCGGAGGCGACAATCTTGCGATTGAACTGCTCATGTCCTTCCAGTCGCAGGGCGTCGCTTTCCGCCGGAATGAAAATACGTCGCTCGTACATCCGTGCGAGAGTAAACAATGCAGCTCCGGCAAGcac                                              >  2:710446/1‑140 (MQ=255)                                                                                          aTACCCACTGGCGCGGAGGCGACAATCTTGCGATTGAACTGCTCATGTCCTTCCAGTCGCAGGGCGTCGCTTTCCGCCGGAATGAAAATACGTCGCTCGTACATCCGTGCGAGAGTAAACAATGCAGCTCCGGCAAGcac                                              >  2:1050529/1‑140 (MQ=255)                                                                                           tACCCACTGGCGCGGAGGCGACAATCTTGCGATTGAACTGCTCATGTCCTTCCAGTCGCAGGGCGTCGCTTTCCGCCGGAATGAAAATACGTCGCTCGTACATCCGTGCGAGAGTAAACAATGCAGCTCCGGCAAGcaca                                             >  1:158460/1‑140 (MQ=255)                                                                                                  tGGCGCGGAGGCGACAATCTTGCGATTGAACTGCTCATGTCCTTCCAGTCGCAGGGCGTCGCTTTCCGCCGGAATGAAAATACGTCGCTCGTACATCCGTGCGAGAGTAAACAATGCAGCTCCGGCAAGCACATTCAGCa                                      >  2:759214/1‑140 (MQ=255)                                                                                                    gcgcGGAGGCGACAATCTTGCGATTGAACTGCTCATGTCCTTCCAGTCGCAGGGCGTCGCTTTCCGCCGGAATGAAAATACGTCGCTCGTACATCCGTGCGAGAGTAAACAATGCAGCTCCGGCAAGCACATTCAGCaaa                                    >  1:77534/1‑140 (MQ=255)                                                                                                     cgcgGAGGCGACAATCTTGCGATTGAACTGCTCATGTCCTTCCAGTCGCAGGGCGTCGCTTTCCGCCGGAATGAAAATACGTCGCTCGTACATCCGTGCGAGAGTAAACAATGCAGCTCCGGCAAGCACATTCAGCaaaa                                   <  1:732881/140‑1 (MQ=255)                                                                                                      gcgGAGGCGACAATCTTGCGATTGAACTGCTCATGTCCTTCCAGTCGCAGGGCGTCGCTTTCCGCCGGAATGAAAATACGTCGCTCGTACATCCGTGCGAGAGTAAACAATGCAGCTCCGGCAAGCACATTCAGCAAAAt                                  <  1:759214/140‑1 (MQ=255)                                                                                                      gcgGAGGCGACAATCTTGCGATTGAACTGCTCATGTCCTTCCAGTCGCAGGGCGTCGCTTTCCGCCGGAATGAAAATACGTCGCTCGTACATCCGTGCGAGAGTAAACAATGCAGCTCCGGCAAGCACATTCAGCAAAAt                                  >  1:1282271/1‑140 (MQ=255)                                                                                                       cgGAGGCGACAATCTTGCGATTGAACTGCTCATGTCCTTCCAGTCGCAGGGCGTCGCTTTCCGCCGGAATGAAAATACGTCGCTCGTACATCCGTGCGAGAGTAAACAATGCAGCTCCGGCAAGCACATTCAGCAAAAt                                  >  2:31735/1‑139 (MQ=255)                                                                                                       cgGAGGCGACAATCTTGCGATTGAACTGCTCATGTCCTTCCAGTCGCAGGGCGTCGCTTTCCGCCGGAATGAAAATACGTCGCTCGTACATCCGTGCGAGAGTAAACAATGCAGCTCCGGCAAGCACATTCAGCAAAAt                                  <  1:31735/139‑1 (MQ=255)                                                                                                            gCGACAATCTTGCGATTGAACTGCTCATGTCCTTCCAGTCGCAGGGCGTCGCTTTCCGCCGGAATGAAAATACGTCGCTCGTACATCCGTGCGAGAGTAAACAATGCAGCTCCGGCAAGCACATTCAGCAAAATTGCGtt                            <  2:1282271/140‑1 (MQ=255)                                                                                                            gCGACAATCTTGCGATTGAACTGCTCATGTCCTTCCAGTCGCAGGGCGTCGCTTTCCGCCGGAATGAAAATACGTCGCTCGTACATCCGTGCGAGAGTAAACAATGCAGCTCCGGCAAGCACATTCAGCAAAATTGCGtt                            >  1:973928/1‑140 (MQ=255)                                                                                                             cGACAATCTTGCGATTGAACTGCTCATGTCCTTCCAGTCGCAGGGCGTCGCTTTCCGCCGGAATGAAAATACGTCGCTCGTACATCCGTGCGAGAGTAAACAATGCAGCTCCGGCAAGCACATTCAGCAAAATTGCGTTa                           <  1:311143/140‑1 (MQ=255)                                                                                                              gACAATCTTGCGATTGAACTGCTCATGTCCTTCCAGTCGCAGGGCGTCGCTTTCCGCCGGAATGAAAATACGTCGCTCGTACATCCGTGCGAGAGTAAACAATGCAGCTCCGGCAAGCACATTCAGCAAAATTGCGTTaa                          >  1:1093071/1‑140 (MQ=255)                                                                                                              gACAATCTTGCGATTGAACTGCTCATGTCCTTCCAGTCGCAGGGCGTCGCTTTCCGCCGGAATGAAAATACGTCGCTCGTACATCCGTGCGAGAGTAAACAATGCAGCTCCGGCAAGCACATTCAGCAAAATTGCGTTaa                          >  1:162417/1‑140 (MQ=255)                                                                                                               aCAATCTTGCGATTGAACTGCTCATGTCCTTCCAGTCGCAGGGCGTCGCTTTCCGCCGGAATGAAAATACGTCGCTCGTACATCCGTGCGAGAGTAAACAATGCAGCTCCGGCAAGCACATTCAGCAAAATTGCGTTAAg                         >  1:522768/1‑140 (MQ=255)                                                                                                                  aTCTTGCGATTGAACTGCTCATGTCCTTCCAGTCGCAGGGCGTCGCTTTCCGCCGGAATGAAAATACGTCGCTCGTACATCCGTGCGAGAGTAAACAATGCAGCTCCGGCAAGCACATTCAGCAAAATTGCGTTAAGGAt                      <  2:704398/140‑1 (MQ=255)                                                                                                                  aTCTTGCGATTGAACTGCTCATGTCCTTCCAGTCGCAGGGCGTCGCTTTCCGCCGGAATGAAAATACGTCGCTCGTACATCCGTGCGAGAGTAAACAATGCAGCTCCGGCAAGCACATTCAGCAAAATTGCGTTAAGGAt                      <  1:961555/140‑1 (MQ=255)                                                                                                                    cTTGCGATTGAACTGCTCATGTCCTTCCAGTCGCAGGGCGTCGCTTTCCGCCGGAATGAAAATACGTCGCTCGTACATCCGTGCGAGAGTAAACAATGCAGCTCCGGCAAGCACATTCAGCAAAATTGCGTTAAGGATCa                    <  2:699691/140‑1 (MQ=255)                                                                                                                               tccgCTCATGTCCTTCCAGTCGCAGGGCGTCGCTTTCCGCCGGAATGAAAATTCGTCGCTCGTACATCCGTGCGAGAGTAAACAATGCAGCTCCGGCAAGCACATTCAGCAAAATTGCGTTAAGGATCAACATGCGAATg         <  2:506536/137‑1 (MQ=255)                                                                                                                               aCTGCTCATGTCCTTCCAGTCGCAGGGCGTCGCTTTCCGCCGGAATGAAAATACGTCGCTCGTACATCCGTGCGAGAGTAAACAATGCAGCTCCGGCAAGCACATTCAGCAAAATTGCGTTAAGGATCAACATGCGAATg         <  2:158460/140‑1 (MQ=255)                                                                                                                                      aTGTCCTTCCAGTCGCAGGGCGTCGCTTTCCGCCGGAATGAAAATACGTCGCTCGTACATCCGTGCGAGAGTAAACAATGCAGCTCCGGCAAGCACATTCAGCAAAATTGCGTTAAGGATCAACATGCGAATGCGTTCCa  <  2:162417/140‑1 (MQ=255)                                                                                                                                          |                                                                                                                                        GGCGGTCCTCATGCGTAAGCATATTGAGATAGGTATGCGCCAGTTCGTTACTTAAAATATTGACGCCATCAGCGGTACGCAAAATGCAGATACCCACTGGCGCGGAGGCGACAATCTTGCGATTGAACTGCTCATGTTCTTCCAGTCGCAGGGCGTCGCTTTCCGCCGGAATGAAAATACGTCGCTCGTACATCCGTGCGAGAGTAAACAATGCAGCTCCGGCAAGCACATTCAGCAAAATTGCGTTAAGGATCAACATGCGAATGCGTTCCA  >  NC_000913/2318679‑2318951

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑