Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	494,020	T→C	I643I (ATT→ATC)	dnaX →	DNA polymerase III subunit tau

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	494,020	0	T	C	100.0%	213.7 / NA	55	I643I (ATT→ATC)	dnaX	DNA polymerase III subunit tau
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base C (26/29);  total (26/29)

GCCGCTGGAGTGGCGTCAGGCGATATACGAAGAAAAACTTGCGCAGGCGCGCGAGTCCATTATTGCGGATAATAATATTCAGACCCTGCGTCGGTTCTTCGATGCGGAGCTGGATGAAGAAAGTATCCGCCCCATTTGATCGTAAGCACAGCTTACGTTCGTCATCCTTAACGTGATTGAGAGAGAAACCTATGTTTGGTAAAGGCGGTCTGGGTAACCTGATGAAGCAAGCCCAGCAGATGCAAGAAAAAATGCAGAAAATG  >  NC_000913/493885‑494147                                                                                                                                        |                                                                                                                                gccgcTGGAGTGGCGTCAGGCGATATACGAAGAAAAACTTGCGCAGGCGCGCGAGTCCATTATTGCGGATAATAATATTCAGACCCTGCGTCGGTTCTTCGATGCGGAGCTGGATGAAGAAAGTATCCGCCCCATCTGAt                                                                                                                             <  2:808360/140‑1 (MQ=255)    gcTGGAGTGGCGTCAGGCGATATACGAAGAAAAACTTGCGCAGGCGCGCGAGTCCATTATTGCGGATAATAATATTCAGACCCTGCGTCGGTTCTTCGATGCGGAGCTGGATGAAGAAAGTATCCGCCCCATCTGATCGt                                                                                                                          <  2:551809/140‑1 (MQ=255)      tGGAGTGGCGTCAGGCGATATACGAAGAAAAACTTGCGCAGGCGCGCGAGTCCATTATTGCGGATAATAATATTCAGACCCTGCGTCGGTTCTTCGATGCGGAGCTGGATGAAGAAAGTATCCGCCCCATCTGATCGTaa                                                                                                                        >  1:1082122/1‑140 (MQ=255)      tGGAGTGGCGTCAGGCGATATACGAAGAAAAACTTGCGCAGGCGCGCGAGTCCATTATTGCGGATAATAATATTCAGACCCTGCGTCGGTTCTTCGATGCGGAGCTGGATGAAGAAAGTATCCGCCCCATCTGATCGTaa                                                                                                                        >  2:736422/1‑140 (MQ=255)             gCGTCAGGCGATATACGAAGAAAAACTTGCGCAGGCGCGCGAGTCCATTATTGCGGATAATAATATTCAGACCCTGCGTCGGTTCTTCGATGCGGAGCTGGATGAAGAAAGTATCCGCCCCATCTGATCGTAAGca                                                                                                                     >  2:969795/1‑136 (MQ=255)             gCGTCAGGCGATATACGAAGAAAAACTTGCGCAGGCGCGCGAGTCCATTATTGCGGATAATAATATTCAGACCCTGCGTCGGTTCTTCGATGCGGAGCTGGATGAAGAAAGTATCCGCCCCATCTGATCGTAAGca                                                                                                                     <  1:969795/136‑1 (MQ=255)             gCGTCAGGCGATATACGAAGAAAAACTTGCGCAGGCGCGCGAGTCCATTATTGCGGATAATAATATTCAGACCCTGCGTCGGTTCTTCGATGCGGAGCTGGATGAAGAAAGTATCCGCCCCATCTGATCGTAAGCACAGc                                                                                                                 >  2:547400/1‑140 (MQ=255)                       atatACGAAGAAAAACTTGCGCAGGCGCGCGAGTCCATTATTGCGGATAATAATATTCAGACCCTGCGTCGGTTCTTCGATGCGGAGCTGGATGAAGAAAGTATCCGCCCCATCTGATCGTAAGCACAGCTTACGTTCGt                                                                                                       <  2:439533/140‑1 (MQ=255)                       atatACGAAGAAAAACTTGCGCAGGCGCGCGAGTCCATTATTGCGGATAATAATATTCAGACCCTGCGTCGGTTCTTCGATGCGGAGCTGGATGAAGAAAGTATCCGCCCCATCTGATCGTAAGCACAGCTTACGTTCGt                                                                                                       <  2:408803/140‑1 (MQ=255)                           aCGAAGAAAAACTTGCGCAGGCGCGCGAGTCCATTATTGCGGATAATAATATTCAGACCCTGCGTCGGTTCTTCGATGCGGAGCTGGATGAAGAAAGTATCCGCCCCATCTGATCGTAAGCACAGCTTACGTTCGTCATc                                                                                                   <  2:827832/140‑1 (MQ=255)                              aagaaAAACTTGCGCAGGCGCGCGAGTCCATTATTGCGGATAATAATATTCAGACCCTGCGTCGGTTCTTCGATGCGGAGCTGGATGAAGAAAGTATCCGCCCCATCTGATCGTAAGCACAGCTTACGTTCGTCATCCtt                                                                                                >  1:725599/1‑140 (MQ=255)                               agaaAAACTTGCGCAGGCGCGCGAGTCCATTATTGCGGATAATAATATTCAGACCCTGCGTCGGTTCTTCGATGCGGAGCTGGATGAAGAAAGTATCCGCCCCATCTGATCGTAAGCACAGCTTACGTTCGTCATCCTTa                                                                                               <  1:980506/140‑1 (MQ=255)                               agaaAAACTTGCGCAGGCGCGCGAGTCCATTATTGCGGATAATAATATTCAGACCCTGCGTCGGTTCTTCGATGCGGAGCTGGATGAAGAAAGTATCCGCCCCATCTGATCGTAAGCACAGCTTACGTTCGTCATCCTTa                                                                                               <  1:656895/140‑1 (MQ=255)                                        tGCGCAGGCGCGCGAGTCCATTATTGCGGATAATAATATTCAGACCCTGCGTCGGTTCTTCGATGCGGAGCTGGATGAAGAAAGTATCCGCCCCATCTGATCGTAAGCACAGCTTACGTTCGTCATCCTTAACGTGATTg                                                                                      <  1:1087962/140‑1 (MQ=255)                                         gcgcAGGCGCGCGAGTCCATTATTGCGGATAATAATATTCAGACCCTGCGTCGGTTCTTCGATGCGGAGCTGGATGAAGAAAGTATCCGCCCCATCTGATCGTAAGCACAGCTTACGTTCGt                                                                                                       <  2:985402/122‑1 (MQ=255)                                         gcgcAGGCGCGCGAGTCCATTATTGCGGATAATAATATTCAGACCCTGCGTCGGTTCTTCGATGCGGAGCTGGATGAAGAAAGTATCCGCCCCATCTGATCGTAAGCACAGCTTACGTTCGt                                                                                                       >  1:985402/1‑122 (MQ=255)                                            cAGGCGCGCGAGTCCATTATTGCGGATAATAATATTCAGACCCTGCGTCGGTTCTTCGATGCGGAGCTGGATGAAGAAAGTATCCGCCCCATCTGATCGTAAGCACAGCTTACGTTCGTCATCCTTAACGTGATTgagag                                                                                  <  2:883691/140‑1 (MQ=255)                                              ggCGCGCGAGTCCATTATTGCGGATAATAATATTCAGACCCTGCGTCGGTTCTTCGATGCGGAGCTGGATGAAGAAAGTATCCGCCCCATCTGATCGTAAGCACAGCTTACGTTCGTCATCCTTAACGTGATTgagagag                                                                                <  1:326961/140‑1 (MQ=255)                                               gcgcgcGAGTCCATTATTGCGGATAATAATATTCAGACCCTGCGTCGGTTCTTCGATGCGGAGCTGGATGAAGAAAGTATCCGCCCCATCTGATCGTAAGCACAGCTTACGTTCGTCATCCTTAACGTGATTgagagaga                                                                               <  1:93954/140‑1 (MQ=255)                                                  cgcgAGTCCATTATTGCGGATAATAATATTCAGACCCTGCGTCGGTTCTTCGATGCGGAGCTGGATGAAGAAAGTATCCGCCCCATCTGATCGTAAGCACAGCTTACGTTCGTCATCCTTAACGTGATTGAGAGAGAAAc                                                                            <  2:1082122/140‑1 (MQ=255)                                                    cgAGTCCATTATTGCGGATAATAATATTCAGACCCTGCGTCGGTTCTTCGATGCGGAGCTGGATGAAGAAAGTATCCGCCCCATCTGATCGTAAGCACAGCTTACGTTCGTCATCCTTAACGTGATTGAGAGAGAAACCt                                                                          <  1:1358212/140‑1 (MQ=255)                                                       gTCCATTATTGCGGATAATAATATTCAGACCCTGCGTCGGTTCTTCGATGCGGAGCTGGATGAAGAAAGTATCCGCCCCATCTGATCGTAAGCACAGCTTACGTTCGTCATCCTTAACGTGATTGAGAGAGAAACCTATg                                                                       >  1:12909/1‑140 (MQ=255)                                                          cATTATTGCGGATAATAATATTCAGACCCTGCGTCGGTTCTTCGATGCGGAGCTGGATGAAGAAAGTATCCGCCCCATCTGATCGTAAGCACAGCTTACGTTCGTCATCCTTAACGTGATTGAGAGAGAAACCTATGttt                                                                    >  1:1269619/1‑140 (MQ=255)                                                                 gCGGATAATAATATTCAGACCCTGCGTCGGTTCTTCGATGCGGAGCTGGATGAAGAAAGTATCCGCCCCATCTGATCGTAAGCACAGCTTACGTTCGTCATCCTTAACGTGATTGAGAGAGAAACCTATGTTTGGTAAAg                                                             >  2:778239/1‑140 (MQ=255)                                                                     ataataataTTCAGACCCTGCGTCGGTTCTTCGATGCGGAGCTGGATGAAGAAAGTATCCGCCCCATCTGATCGTAAGCACAGCTTACGTTCGTCATCCTTAACGTGATTGAGAGAGAAACCTATGTTTGGTAAAGGCgg                                                         <  1:366568/140‑1 (MQ=255)                                                                     ataataataTTCAGACCCTGCGTCGGTTCTTCGATGCGGAGCTGGATGAAGAAAGTATCCGCCCCATCTGATCGTAAGCACAGCTTACGTTCGTCATCCTTAACGTGATTGAGAGAGAAACCTATGTTTGGTAAAGGCgg                                                         <  1:485550/140‑1 (MQ=255)                                                                           atatTCAGACCCTGCGTCGGTTCTTCGATGCGGAGCTGGATGAAGAAAGTATCCGCCCCATCTGATCGTAAGCACAGCTTACGTTCGTCATCCTTAACGTGATTGAGAGAGAAACCTATGTTTGGTAAAGGCGGTCTggg                                                   <  2:12909/140‑1 (MQ=255)                                                                                cAGACCCTGCGTCGGTTCTTCGATGCGGAGCTGGATGAAGAAAGTATCCGCCCCATCTGATCGTAAGCACAGCTTACGTTCGTCATCCTTAACGTGATTGAGAGAGAAACCTATGTTTGGTAAAGGCGGTCTGGGTAAcc                                              >  1:1195144/1‑140 (MQ=255)                                                                                   aCCCTGCGTCGGTTCTTCGATGCGGAGCTGGATGAAGAAAGTATCCGCCCCATCTGATCGTAAGCACAGCTTACGTTCGTCATCCTTAACGTGATTGAGAGAGAAACCTATGTTTGGTAAAGGCGGTCTGGGTAAc                                               >  2:592032/1‑136 (MQ=255)                                                                                   aCCCTGCGTCGGTTCTTCGATGCGGAGCTGGATGAAGAAAGTATCCGCCCCATCTGATCGTAAGCACAGCTTACGTTCGTCATCCTTAACGTGATTGAGAGAGAAACCTATGTTTGGTAAAGGCGGTCTGGGTAAc                                               <  1:592032/136‑1 (MQ=255)                                                                                   aCCCTGCGTCGGTTCTTCGATGCGGAGCTGGATGAAGAAAGTATCCGCCCCATCTGATCGTAAGCACAGCTTACGTTCGTCATCCTTAACGTGATTGAGAGAGAAACCTATGTTTGGTAAAGGCGGTCTGGGTAACCtga                                           >  1:438002/1‑140 (MQ=255)                                                                                   aCCCTGCGTCGGTTCTTCGATGCGGAGCTGGATGAAGAAAGTATCCGCCCCATCTGATCGTAAGCACAGCTTACGTTCGTCATCCTTAACGTGATTGAGAGAGAAACCTATGTTTGGTAAAGGCGGTCTGGGTAACCtga                                           >  2:935241/1‑140 (MQ=255)                                                                                       tGCGTCGGTTCTTCGATGCGGAGCTGGATGAAGAAAGTATCCGCCCCATCTGATCGTAAGCACAGCTTACGTTCGTCATCCTTAACGTGATTGAGAGAGAAACCTATGTTTGGTAAAGGCGGTCTGGGTa                                                 <  1:380160/130‑1 (MQ=255)                                                                                       tGCGTCGGTTCTTCGATGCGGAGCTGGATGAAGAAAGTATCCGCCCCATCTGATCGTAAGCACAGCTTACGTTCGTCATCCTTAACGTGATTGAGAGAGAAACCTATGTTTGGTAAAGGCGGTCTGGGTa                                                 >  2:380160/1‑130 (MQ=255)                                                                                       tGCGTCGGTTCTTCGATGCGGAGCTGGATGAAGAAAGTATCCGCCCCATCTGATCGTAAGCACAGCTTACGTTCGTCATCCTTAACGTGATTGAGAGAGAAACCTATGTTTGGTAAAGGCGGTCTGGGTAAc                                               >  1:437884/1‑132 (MQ=255)                                                                                       tGCGTCGGTTCTTCGATGCGGAGCTGGATGAAGAAAGTATCCGCCCCATCTGATCGTAAGCACAGCTTACGTTCGTCATCCTTAACGTGATTGAGAGAGAAACCTATGTTTGGTAAAGGCGGTCTGGGTAAc                                               <  2:437884/132‑1 (MQ=255)                                                                                       tGCGTCGGTTCTTCGATGCGGAGCTGGATGAAGAAAGTATCCGCCCCATCTGATCGTAAGCACAGCTTACGTTCGTCATCCTTAACGTGATTGAGAGAGAAACCTATGTTTGGTAAAGGCGGTCTGGGTAACCTGATGaa                                       >  2:814866/1‑140 (MQ=255)                                                                                       tGCGTCGGTTCTTCGATGCGGAGCTGGATGAAGAAAGTATCCGCCCCATCTGATCGTAAGCACAGCTTACGTTCGTCATCCTTAACGTGATTGAGAGAGAAACCTATGTTTGGTAAAGGCGGTCTGGGTAACCTGATGaa                                       >  1:1155902/1‑140 (MQ=255)                                                                                        gCGTCGGTTCTTCGATGCGGAGCTGGATGAAGAAAGTATCCGCCCCATCTGATCGTAAGCACAGCTTACGTTCGTCATCCTTAACGTGATTGAGAGAGAAACCTATGTTTGGTAAAGGCGGTCTGGGTAACCTGATGaag                                      >  1:590375/1‑140 (MQ=255)                                                                                         cGTCGGTTCTTCGATGCGGAGCTGGATGAAGAAAGTATCCGCCCCATCTGATCGTAAGCACAGCTTACGTTCGTCATCCTTAACGTGATTGAGAGAGAAACCTATGTTTGGTAAAGGCGGTCTGGGTAACCTGATGaagc                                     >  1:1109807/1‑140 (MQ=255)                                                                                          gTCGGTTCTTCGATGCGGAGCTGGATGAAGAAAGTATCCGCCCCATCTGATCGTAAGCACAGCTTACGTTCGTCATCCTTAACGTGATTGAGAGAGAAACCTATGTTTGGTAAAGGCGGTCTGGGTAACCTGATGaagca                                    <  2:1269619/140‑1 (MQ=255)                                                                                            cGGTTCTTCGATGCGGAGCTGGATGAAGAAAGTATCCGCCCCATCTGATCGTAAGCACAGCTTACGTTCGTCATCCTTAACGTGATTGAGAGAGAAACCTATGTTTGGTAAAGGCGGTCTGGGTAACCTGATGaagcaag                                  <  1:814866/140‑1 (MQ=255)                                                                                             ggTTCTTCGATGCGGAGCTGGATGAAGAAAGTATCCGCCCCATCTGATCGTAAGCACAGCTTACGTTCGTCATCCTTAACGTGATTGAGAGAGAAACCTATGTTTGGTAAAGGCGGTCTGGGTAACCTGATGaagcaagc                                 >  2:863745/1‑140 (MQ=255)                                                                                             ggTTCTTCGATGCGGAGCTGGATGAAGAAAGTATCCGCCCCATCTGATCGTAAGCACAGCTTACGTTCGTCATCCTTAACGTGATTGAGAGAGAAACCTATGTTTGGTAAAGGCGGTCTGGGTAACCTGATGaa                                       <  2:618197/134‑1 (MQ=255)                                                                                             ggTTCTTCGATGCGGAGCTGGATGAAGAAAGTATCCGCCCCATCTGATCGTAAGCACAGCTTACGTTCGTCATCCTTAACGTGATTGAGAGAGAAACCTATGTTTGGTAAAGGCGGTCTGGGTAACCTGATGaa                                       >  1:618197/1‑134 (MQ=255)                                                                                              gTTCTTCGATGCGGAGCTGGATGAAGAAAGTATCCGCCCCATCTGATCGTAAGCACAGCTTACGTTCGTCATCCTTAACGTGATTGAGAGAGAAACCTATGTTTGGTAAAGGCGGTCTGGGTAACCTGATGAAGCAAGcc                                <  1:736422/140‑1 (MQ=255)                                                                                                   tcGATGCGGAGCTGGATGAAGAAAGTATCCGCCCCATCTGATCGTAAGCACAGCTTACGTTCGTCATCCTTAACGTGATTGAGAGAGAAACCTATGTTTGGTAAAGGCGGTCTGGGTAACCTGATGAAGCAAGCCcagca                           >  1:978850/1‑140 (MQ=255)                                                                                                         cGGAGCTGGATGAAGAAAGTATCCGCCCCATCTGATCGTAAGCACAGCTTACGTTCGTCATCCTTAACGTGATTGAGAGAGAAACCTATGTTTGGTAAAGGCGGTCTGGGTAACCTGATGAAGCAAGCCCAGCAGATGCa                     >  2:60917/1‑140 (MQ=255)                                                                                                         cGGAGCTGGATGAAGAAAGTATCCGCCCCATCTGATCGTAAGCACAGCTTACGTTCGTCATCCTTAACGTGATTGAGAGAGAAACCTATGTTTGGTAAAGGCGGTCTGGGTAACCTGATGAAGCAAGCCCAGCAGATGCa                     <  1:547400/140‑1 (MQ=255)                                                                                                            aGCTGGATGAAGAAAGTATCCGCCCCATCTGATCGTAAGCACAGCTTACGTTCGTCATCCTTAACGTGATTGAGAGAGAAACCTATGTTTGGTAAAGGCGGTCTGGGTAACCTGATGAAGCAAGCCCAGCAGATGCAAGa                  >  1:284343/1‑140 (MQ=255)                                                                                                            aGCTGGATGAAGAAAGTATCCGCCCCATCTGATCGTAAGCACAGCTTACGTTCGTCATCCTTAACGTGATTGAGAGAGAAACCTATGTTTGGTAAAGGCGGTCTGGGTAACCTGATGAAGCAAGCCCAGCAGATGCAAGa                  >  2:1118440/1‑140 (MQ=255)                                                                                                                     aagaaAGTATCCGCCCCATCTGATCGTAAGCACAGCTTACGTTCGTCATCCTTAACGTGATTGAGAGAGAAACCTATGTTTGGTAAAGGCGGTCTGGGTAACCTGATGAAGCAAGCCCAGCAGATGCAAGAAaaaatgca         <  2:438002/140‑1 (MQ=255)                                                                                                                      agaaAGTATCCGCCCCATCTGATCGTAAGCACAGCTTACGTTCGTCATCCTTAACGTGATTGAGAGAGAAACCTATGTTTGGTAAAGGCGGTCTGGGTAACCTGATGAAGCAAGCCCAGCAGATGCAAGAAaaaatgcag        >  1:919868/1‑140 (MQ=255)                                                                                                                        aaaGTATCCGCCCCATCTGATCGTAAGCACAGCTTACGTTCGTCATCCTTAACGTGATTGAGAGAGAAACCTATGTTTGGTAAAGGCGGTCTGGGTAACCTGATGAAGCAAGCCCAGCAGATGCAAGAAaaaatgcagaa      <  1:1118440/140‑1 (MQ=255)                                                                                                                            tATCCGCCCCATCTGATCGTAAGCACAGCTTACGTTCGTCATCCTTAACGTGATTGAGAGAGAAACCTATGTTTGGTAAAGGCGGTCTGGGTAACCTGATGAAGCAAGCCCAGCAGATGCAAGAAaaaatgcagaaaatg  <  2:978850/140‑1 (MQ=255)                                                                                                                                        |                                                                                                                                GCCGCTGGAGTGGCGTCAGGCGATATACGAAGAAAAACTTGCGCAGGCGCGCGAGTCCATTATTGCGGATAATAATATTCAGACCCTGCGTCGGTTCTTCGATGCGGAGCTGGATGAAGAAAGTATCCGCCCCATTTGATCGTAAGCACAGCTTACGTTCGTCATCCTTAACGTGATTGAGAGAGAAACCTATGTTTGGTAAAGGCGGTCTGGGTAACCTGATGAAGCAAGCCCAGCAGATGCAAGAAAAAATGCAGAAAATG  >  NC_000913/493885‑494147

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑