breseq  version 0.35.4  revision f352f80f4bc9
mutation predictions | marginal predictions | summary statistics | genome diff | command line log

Predicted mutations
evidence position mutation annotation gene description
MC JC 257,908 Δ776 bp insB9[crl] insB9, insA9, [crl]
RA 697,132 C→A noncoding (2/77 nt) metT ← tRNA‑Met
RA 889,923 A→G intergenic (‑104/‑166) ybjL ← / → ybjM putative transport protein YbjL/putative inner membrane protein
RA 1,196,220 C→T H366H (CAC→CAT icd → isocitrate dehydrogenase
RA 1,196,232 C→T T370T (ACC→ACT icd → isocitrate dehydrogenase
RA 1,196,245 T→C L375M (TTA→CTG)  icd → isocitrate dehydrogenase
RA 1,196,247 A→G L375M (TTA→CTG icd → isocitrate dehydrogenase
MC JC 1,299,499 Δ1,199 bp insH21 insH21
JC JC 1,879,829 Δ1 bp :: IS186 (–) +6 bp :: Δ1 bp coding (115‑120/360 nt) yeaR ← DUF1971 domain‑containing protein YeaR
MC JC 1,978,503 Δ776 bp insB‑5insA‑5 insB‑5, insA‑5
RA 2,173,363 Δ2 bp pseudogene (915‑916/1358 nt) gatC ← galactitol‑specific PTS enzyme IIC component
JC JC 2,536,312 Δ1 bp :: IS186 (+) +6 bp :: Δ1 bp coding (479‑484/510 nt) crr → Enzyme IIA(Glc)
RA 3,354,487 C→T intergenic (‑437/‑238) yhcC ← / → gltB radical SAM family oxidoreductase YhcC/glutamate synthase subunit GltB
RA 3,560,455 +G pseudogene (151/758 nt) glpR ← DNA‑binding transcriptional repressor GlpR
RA 3,774,701 A→G E93G (GAA→GGA)  mtlD → mannitol‑1‑phosphate 5‑dehydrogenase
RA 3,815,883 +C coding (667/687 nt) rph ← truncated RNase PH
RA 4,175,937 A→G intergenic (+108/‑7) thrT → / → tufB tRNA‑Thr/translation elongation factor Tu 2
RA 4,296,381 +GC intergenic (+587/+55) gltP → / ← yjcO glutamate/aspartate : H(+) symporter GltP/Sel1 repeat‑containing protein YjcO

Unassigned missing coverage evidence
   seq id start end size ←reads reads→ gene description
* * ÷ NC_000913 1196277 1211432 15156 19 [18] [18] 21 [icd]–[icdC] [icd],ymfD,ymfE,lit,intE,xisE,ymfH,ymfI,ymfJ,ymfK,ymfT,ymfL,ymfM,oweE,ymfN,aaaE,ymfR,beeE,jayE,ymfQ,ycfK,tfaP,tfaE,stfE,pinE,mcrA,[icdC]
* * ÷ NC_000913 3423750–3424233 3424525–3424239 7–776 19 [17] [17] 23 [rrfD]–[rrlD] [rrfD],[rrlD]

Unassigned new junction evidence
  seq id position reads (cov) reads (cov) score skew freq annotation gene product
* ? NC_000913 226373 =NA (NA)77 (1.140) 52/264 0.2 100% noncoding (615/2904 nt) rrlH 23S ribosomal RNA
?NC_000913 = 226385 0 (0.000)noncoding (627/2904 nt) rrlH 23S ribosomal RNA
* ? NC_000913 3363001 =103 (1.470)109 (1.570) 62/272 0.1 67.7% coding (195/2382 nt) yhcD putative fimbrial usher protein YhcD
?NC_000913 3766087 = 2 (0.030)coding (3905/4134 nt) rhsA rhs element protein RhsA
* ? NC_000913 = 3653230NA (NA)60 (0.860) 50/274 0.2 95.2% noncoding (1/1195 nt) IS5 repeat region
?NC_000913 = 3766089 3 (0.040)coding (3907/4134 nt) rhsA rhs element protein RhsA
* ? NC_000913 = 454269045 (0.640)19 (0.290) 16/256 2.6 26.2% intergenic (+57/‑425) fimE/fimA regulator for fimA/type 1 fimbriae major subunit
?NC_000913 = 4542986 65 (0.990)intergenic (+353/‑129) fimE/fimA regulator for fimA/type 1 fimbriae major subunit