Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	2,106,616	A→G	F204S (TTC→TCC)	wbbH ←	putative O‑antigen polymerase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	2,106,616	0	A	G	100.0%	357.7 / NA	92	F204S (TTC→TCC)	wbbH	putative O‑antigen polymerase
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base G (49/43);  total (49/43)

GGCTGACCAAATACATGGATAGATAATATGCCATCCCCCCAGGCAGTCCACGTAAAAAGAGCATATACAAGGAGAATAGAACACCTACAGCTGTAATAAGATAAACATAATGTTTTACTCTATTAACACCTACGATGAATGCATAAGAGATGATAACCATAAAGACAATTTGCTTACCAGTATTCAGTATAATTGCAAAGATGAATACAATAAAAACAAGTAAAGTAAATGTTTTACTTACCTTTGTATTAGTAAATTTTTTAGACCAAATA  >  NC_000913/2106479‑2106750                                                                                                                                          |                                                                                                                                       ggCTGACCAAATACATGGATAGATAATATGCCATCCCCCCAGGCAGTCCACGTAAAAAGAGCATATACAAGGAGAATAGAACACCTACAGCTGTAATAAGATAAACATAATGTTTTACTCTATTAACACCTACGATGGAt                                                                                                                                      >  1:1227173/1‑140 (MQ=255)    tGACCAAATACATGGATAGATAATATGCCATCCCCCCAGGCAGTCCACGTAAAAAGAGCATATACAAGGAGAATAGAACACCTACAGCTGTAATAAGATAAACATAATGTTTTACTCTATTAACACCTACGATGGATGCa                                                                                                                                   <  1:532727/140‑1 (MQ=255)     gACCAAATACATGGATAGATAATATGCCATCCCCCCAGGCAGTCCACGTAAAAAGAGCATATACAAGGAGAATAGAACACCTACAGCTGTAATAAGATAAACATAATGTTTTACTCTATTAACACCTACgatggatg                                                                                                                                     >  2:19319/1‑137 (MQ=255)     gACCAAATACATGGATAGATAATATGCCATCCCCCCAGGCAGTCCACGTAAAAAGAGCATATACAAGGAGAATAGAACACCTACAGCTGTAATAAGATAAACATAATGTTTTACTCTATTAACACCTACgatggatg                                                                                                                                     <  1:19319/137‑1 (MQ=255)     gACCAAATACATGGATAGATAATATGCCATCCCCCCAGGCAGTCCACGTAAAAAGAGCATATACAAGGAGAATAGAACACCTACAGCTGTAATAAGATAAACATAATGTTTTACTCTATTAACACCTACGATGGATGCAt                                                                                                                                  >  2:1143688/1‑140 (MQ=255)     gACCAAATACATGGATAGATAATATGCCATCCCCCCAGGCAGTCCACGTAAAAAGAGCATATACAAGGAGAATAGAACACCTACAGCTGTAATAAGATAAACATAATGTTTTACTCTATTAACACCTACGATGGATGCAt                                                                                                                                  >  1:499921/1‑140 (MQ=255)      aCCAAATACATGGATAGATAATATGCCATCCCCCCAGGCAGTCCACGTAAAAAGAGCATATACAAGGAGAATAGAACACCTACAGCTGTAATAAGATAAACATAATGTTTTACTCTATTAACACCTACGATGGATGCATa                                                                                                                                 >  2:517750/1‑140 (MQ=255)      aCCAAATACATGGATAGATAATATGCCATCCCCCCAGGCAGTCCACGTAAAAAGAGCATATACAAGGAGAATAGAACACCTACAGCTGTAATAAGATAAACATAATGTTTTACTCTATTAACACCTACGATGGATGCATa                                                                                                                                 <  1:930260/140‑1 (MQ=255)         aaaTACATGGATAGATAATATGCCATCCCCCCAGGCAGTCCACGTAAAAAGAGCATATACAAGGAGAATAGAACACCTACAGCTGTAATAAGATAAACATAATGTTTTACTCTATTAACACCTACGATGGATGCATAaga                                                                                                                              <  2:626287/140‑1 (MQ=255)           aTACATGGATAGATAATATGCCATCCCCCCAGGCAGTCCACGTAAAAAGAGCATATACAAGGAGAATAGAACACCTACAGCTGTAATAAGATAAACATAATGTTTTACTCTATTAACACCTACGATGGATGCATAAgaga                                                                                                                            >  2:1370641/1‑140 (MQ=255)                tGGATAGATAATATGCCATCCCCCCAGGCAGTCCACGTAAAAAGAGCATATACAAGGAGAATAGAACACCTACAGCTGTAATAAGATAAACATAATGTTTTACTCTATTAACACCTACgatggatg                                                                                                                                     <  2:346475/126‑1 (MQ=255)                tGGATAGATAATATGCCATCCCCCCAGGCAGTCCACGTAAAAAGAGCATATACAAGGAGAATAGAACACCTACAGCTGTAATAAGATAAACATAATGTTTTACTCTATTAACACCTACgatggatg                                                                                                                                     >  1:346475/1‑126 (MQ=255)                tGGATAGATAATATGCCATCCCCCCAGGCAGTCCACGTAAAAAGAGCATATACAAGGAGAATAGAACACCTACAGCTGTAATAAGATAAACATAATGTTTTACTCTATTAACACCTACGATGGATGCATAAGAGATGATa                                                                                                                       <  2:431834/140‑1 (MQ=255)                tGGATAGATAATATGCCATCCCCCCAGGCAGTCCACGTAAAAAGAGCATATACAAGGAGAATAGAACACCTACAGCTGTAATAAGATAAACATAATGTTTTACTCTATTAACACCTACGATGGATGCATAAGAGATGATa                                                                                                                       >  1:1349336/1‑140 (MQ=255)                    tagataATATGCCATCCCCCCAGGCAGTCCACGTAAAAAGAGCATATACAAGGAGAATAGAACACCTACAGCTGTAATAAGATAAACATAATGTTTTACTCTATTAACACCTACGATGGATGCATAAGAGATGATAACCa                                                                                                                   >  1:1294768/1‑140 (MQ=255)                      gataATATGCCATCCCCCCAGGCAGTCCACGTAAAAAGAGCATATACAAGGAGAATAGAACACCTACAGCTGTAATAAGATAAACATAATGTTTTACTCTATTAACACCTACGATGGATGCATAAGAGATGATAACCATa                                                                                                                 >  1:672912/1‑140 (MQ=255)                       ataataTGCCATCCCCCCAGGCAGTCCACGTAAAAAGAGCATATACAAGGAGAATAGAACACCTACAGCTGTAATAAGATAAACATAATGTTTTACTCTATTAACACCTACGATGGATGCATAAGAGATGATAACCATaa                                                                                                                >  2:447200/1‑140 (MQ=255)                       ataataTGCCATCCCCCCAGGCAGTCCACGTAAAAAGAGCATATACAAGGAGAATAGAACACCTACAGCTGTAATAAGATAAACATAATGTTTTACTCTATTAACACCTACGATGGATGCATAAGAGATGATAACCATaa                                                                                                                >  2:1109663/1‑140 (MQ=255)                       ataataTGCCATCCCCCCAGGCAGTCCACGTAAAAAGAGCATATACAAGGAGAATAGAACACCTACAGCTGTAATAAGATAAACATAATGTTTTACTCTATTAACACCTACGATGGATGCATAAGAGATGATAACCATaa                                                                                                                <  2:1349336/140‑1 (MQ=255)                             tGCCATCCCCCCAGGCAGTCCACGTAAAAAGAGCATATACAAGGAGAATAGAACACCTACAGCTGTAATAAGATAAACATAATGTTTTACTCTATTAACACCTACGATGGATGCATAAGAGATGATa                                                                                                                       >  2:1282165/1‑127 (MQ=255)                             tGCCATCCCCCCAGGCAGTCCACGTAAAAAGAGCATATACAAGGAGAATAGAACACCTACAGCTGTAATAAGATAAACATAATGTTTTACTCTATTAACACCTACGATGGATGCATAAGAGATGATa                                                                                                                       <  1:1282165/127‑1 (MQ=255)                             tGCCATCCCCCCAGGCAGTCCACGTAAAAAGAGCATATACAAGGAGAATAGAACACCTACAGCTGTAATAAGATAAACATAATGTTTTACTCTATTAACACCTACGATGGATGCATAAGAGATGATAACCATAAAGACaa                                                                                                          >  2:845817/1‑140 (MQ=255)                              gCCATCCCCCCAGGCAGTCCACGTAAAAAGAGCATATACAAGGAGAATAGAACACCTACAGCTGTAATAAGATAAACATAATGTTTTACTCTATTAACACCTACGATGGATGCATAAGAGATGATAACCATAAAGACAAt                                                                                                         >  2:1177882/1‑140 (MQ=255)                              gCCATCCCCCCAGGCAGTCCACGTAAAAAGAGCATATACAAGGAGAATAGAACACCTACAGCTGTAATAAGATAAACATAATGTTTTACTCTATTAACACCTACGATGGATGCATAAGAGATGATAACCATAAAGACAAt                                                                                                         <  1:1138032/140‑1 (MQ=255)                               ccATCCCCCCAGGCAGTCCACGTAAAAAGAGCATATACAAGGAGAATAGAACACCTACAGCTGTAATAAGATAAACATAATGTTTTACTCTATTAACACCTACGATGGATGCATAAGAGATGATAACCATAAAGACAAtt                                                                                                        <  1:845817/140‑1 (MQ=255)                                 aTCCCCCCAGGCAGTCCACGTAAAAAGAGCATATACAAGGAGAATAGAACACCTACAGCTGTAATAAGATAAACATAATGTTTTACTCTATTAACACCTACGATGGATGCATAAGAGATGATAACCATAAAGACAATTTg                                                                                                      <  2:471571/140‑1 (MQ=255)                                 aTCCCCCCAGGCAGTCCACGTAAAAAGAGCATATACAAGGAGAATAGAACACCTACAGCTGTAATAAGATAAACATAATGTTTTACTCTATTAACACCTACGATGGATGCATAAGAGATGATAACCATAAAGACAATTTg                                                                                                      >  2:1284530/1‑140 (MQ=255)                                   ccccccAGGCAGTCCACGTAAAAAGAGCATATACAAGGAGAATAGAACACCTACAGCTGTAATAAGATAAACATAATGTTTTACTCTATTAACACCTACGATGGATGCATAAGAGATGATAACCATAAAGACAAt                                                                                                         >  2:875119/1‑135 (MQ=255)                                   ccccccAGGCAGTCCACGTAAAAAGAGCATATACAAGGAGAATAGAACACCTACAGCTGTAATAAGATAAACATAATGTTTTACTCTATTAACACCTACGATGGATGCATAAGAGATGATAACCATAAAGACAAt                                                                                                         <  1:875119/135‑1 (MQ=255)                                   ccccccAGGCAGTCCACGTAAAAAGAGCATATACAAGGAGAATAGAACACCTACAGCTGTAATAAGATAAACATAATGTTTTACTCTATTAACACCTACGATGGATGCATAAGAGATGATAACCATAAAGACAATTTGCt                                                                                                    <  2:1076436/140‑1 (MQ=255)                                   ccccccAGGCAGTCCACGTAAAAAGAGCATATACAAGGAGAATAGAACACCTACAGCTGTAATAAGATAAACATAATGTTTTACTCTATTAACACCTACGATGGATGCATAAGAGATGATAACCATAAAGACAATTTGCt                                                                                                    <  2:285631/140‑1 (MQ=255)                                   ccccccAGGCAGTCCACGTAAAAAGAGCATATACAAGGAGAATAGAACACCTACAGCTGTAATAAGATAAACATAATGTTTTACTCTATTAACACCTACGATGGATGCATAAGAGATGATAACCATAAAGACAATTTGCt                                                                                                    <  2:484697/140‑1 (MQ=255)                                   ccccccAGGCAGTCCACGTAAAAAGAGCATATACAAGGAGAATAGAACACCTACAGCTGTAATAAGATAAACATAATGTTTTACTCTATTAACACCTACGATGGATGCATAAGAGATGATAACCATAAAGACAATTTGCt                                                                                                    <  1:1284530/140‑1 (MQ=255)                                   ccccccAGGCAGTCCACGTAAAAAGAGCATATACAAGGAGAATAGAACACCTACAGCTGTAATAAGATAAACATAATGTTTTACTCTATTAACACCTACGATGGATGCATAAGAGATGATAACCATAAAGACAATTTGCt                                                                                                    <  2:550265/140‑1 (MQ=255)                                      cccAGGCAGTCCACGTAAAAAGAGCATATACAAGGAGAATAGAACACCTACAGCTGTAATAAGATAAACATAATGTTTTACTCTATTAACACCTACGATGGATGCATAAGAGATGATAACCATAAAGACAATTTGCTTAc                                                                                                 <  2:1294768/140‑1 (MQ=255)                                        cAGGCAGTCCACGTAAAAAGAGCATATACAAGGAGAATAGAACACCTACAGCTGTAATAAGATAAACATAATGTTTTACTCTATTAACACCTACGATGGATGCATAAGAGATGATAACCATAAAGACAATTTGCTTACCa                                                                                               <  2:485360/140‑1 (MQ=255)                                           gCAGTCCACGTAAAAAGAGCATATACAAGGAGAATAGAACACCTACAGCTGTAATAAGATAAACATAATGTTTTACTCTATTAACACCTACGATGGATGCATAAGAGATGATAACCATAAAGACAATTTGCTTACCAGTa                                                                                            <  1:447200/140‑1 (MQ=255)                                             aGTCCACGTAAAAAGAGCATATACAAGGAGAATAGAACACCTACAGCTGTAATAAGATAAACATAATGTTTTACTCTATTAACACCTACGATGGATGCATAAGAGATGATAACCATAAAGACAATTTGCTTACCAGTAtt                                                                                          <  2:964842/140‑1 (MQ=255)                                               tCCACGTAAAAAGAGCATATACAAGGAGAATAGAACACCTACAGCTGTAATAAGATAAACATAATGTTTTACTCTATTAACACCTACGATGGATGCATAAGAGATGATAACCATAAAGACAATTTGCTTACCAGTATTCa                                                                                        >  2:585629/1‑140 (MQ=255)                                               tCCACGTAAAAAGAGCATATACAAGGAGAATAGAACACCTACAGCTGTAATAAGATAAACATAATGTTTTACTCTATTAACACCTACGATGGATGCATAAGAGATGATAACCATAAAGACAATTTGCTTACCAGTATTCa                                                                                        >  1:1321165/1‑140 (MQ=255)                                               tCCACGTAAAAAGAGCATATACAAGGAGAATAGAACACCTACAGCTGTAATAAGATAAACATAATGTTTTACTCTATTAACACCTACGATGGATGCATAAGAGATGATAACCATAAAGACAATTTGCTTACCAGTATTCa                                                                                        >  2:1204168/1‑140 (MQ=255)                                                   cGTAAAAAGAGCATATACAAGGAGAATAGAACACCTACAGCTGTAATAAGATAAACATAATGTTTTACTCTATTAACACCTACGATGGATGCATAAGAGATGATAACCATAAAGACAATTTGCTTACCAGTATTCAGtat                                                                                    <  2:672912/140‑1 (MQ=255)                                                   cGTAAAAAGAGCATATACAAGGAGAATAGAACACCTACAGCTGTAATAAGATAAACATAATGTTTTACTCTATTAACACCTACGATGGATGCATAAGAGATGATAACCATAAAGACAATTTGCTTACCAGTATTCAGtat                                                                                    <  2:1227173/140‑1 (MQ=255)                                                    gTAAAAAGAGCATATACAAGGAGAATAGAACACCTACAGCTGTAATAAGATAAACATAATGTTTTACTCTATTAACACCTACGATGGATGCATAAGAGATGATAACCATAAAGACAATTTGCTTACCAGTATTCAGtata                                                                                   >  1:664500/1‑140 (MQ=255)                                                          agagCATATACAAGGAGAATAGAACACCTACAGCTGTAATAAGGTAAACATAATGTTTTACTCTATTAACACCTACGATGGATGCATAAGAGATGATAACCATAAAGACAATTTGCTTACCAGTATTCAGTATAATTGCa                                                                             <  2:471434/140‑1 (MQ=255)                                                          agagCATATACAAGGAGAATAGAACACCTACAGCTGTAATAAGATAAACATAATGTTTTACTCTATTAACACCTACGATGGATGCATAAGAGATGATAACCATAAAGACAATTTGCTTACCAGTATTCAGTATAATTGCa                                                                             <  1:1174144/140‑1 (MQ=255)                                                                  taCAAGGAGAATAGAACACCTACAGCTGTAATAAGATAAACATAATGTTTTACTCTATTAACACCTACGATGGATGCATAAGAGATGATAACCATAAAGACAATTTGCTTACCAGTATTCAGTATAATTGCAAAGATGaa                                                                     <  2:499921/140‑1 (MQ=255)                                                                  taCAAGGAGAATAGAACACCTACAGCTGTAATAAGATAAACATAATGTTTTACTCTATTAACACCTACGATGGATGCATAAGAGATGATAACCATAAAGACAATTTGCTTACCAGTATTCAGTATAATTGCAAAGATGaa                                                                     <  1:1370641/140‑1 (MQ=255)                                                                  taCAAGGAGAATAGAACACCTACAGCTGTAATAAGATAAACATAATGTTTTACTCTATTAACACCTACGATGGATGCATAAGAGATGATAACCATAAAGACAATTTGCTTACCAGTATTCAGTATAATTGCAAAGATGaa                                                                     <  2:681231/140‑1 (MQ=255)                                                                   aCAAGGAGAATAGAACACCTACAGCTGTAATAAGATAAACATAATGTTTTACTCTATTAACACCTACGATGGATGCATAAGAGATGATAACCATAAAGACAATTTGCTTACCAGTATTCAGTATAATTGCAAAGATGAAt                                                                    <  2:1321165/140‑1 (MQ=255)                                                                      aGGAGAATAGAACACCTACATCTGTAATAAGATAAACATAATGTTTTACTCTATTAACACCTACGATGGATGCATAAGAGATGATAACCATAAAGACAATTTGCTTACCAGTATTCAGTATAATTGCAAAGATGAATACa                                                                 >  1:293452/1‑140 (MQ=255)                                                                        gagaATAGAACACCTACAGCTGTAATAAGATAAACATAATGTTTTACTCTATTAACACCTACGATGGATGCATAAGAGATGATAACCATAAAGACAATTTGCTTACCAGTATTCAGTATAATTGCAAAGATGAATACAAt                                                               >  2:1107913/1‑140 (MQ=255)                                                                         agaATAGAACACCTACAGCTGTAATAAGATAAACATAATGTTTTACTCTATTAACACCTACGATGGATGCATAAGAGATGATAACCATAAAGACAATTTGCTTACCAGTATTCAGTATAATTGCAAAGAt                                                                        <  1:714767/130‑1 (MQ=255)                                                                         agaATAGAACACCTACAGCTGTAATAAGATAAACATAATGTTTTACTCTATTAACACCTACGATGGATGCATAAGAGATGATAACCATAAAGACAATTTGCTTACCAGTATTCAGTATAATTGCAAAGAt                                                                        >  2:714767/1‑130 (MQ=255)                                                                           aaTAGAACACCTACAGCTGTAATAAGATAAACATAATGTTTTACTCTATTAACACCTACGATGGATGCATAAGAGATGATAACCATAAAGACAATTTGCTTACCAGTATTCAGTATAATTGCAAAGATGAATACAATaaa                                                            >  2:1254422/1‑140 (MQ=255)                                                                            aTAGAACACCTACAGCTGTAATAAGATAAACATAATGTTTTACTCTATTAACACCTACGATGGATGCATAAGAGATGATAACCATAAAGACAATTTGCTTACCAGTATTCAGTATAATTGCAAAGATGAATACAATaaaa                                                           >  1:214168/1‑140 (MQ=255)                                                                            aTAGAACACCTACAGCTGTAATAAGATAAACATAATGTTTTACTCTATTAACACCTACGATGGATGCATAAGAGATGATAACCATAAAGACAATTTGCTTACCAGTATTCAGTATAATTGCAAAGATGAATACAATaaaa                                                           >  1:655868/1‑140 (MQ=255)                                                                               gAACACCTACAGCTGTAATAAGATAAACATAATGTTTTACTCTATTAACACCTACGATGGATGCATAAGAGATGATAACCATAAAGACAATTTGCTTACCAGTATTCAGTATAATTGCAAAGATGAATACAATAAAAACa                                                        >  1:772117/1‑140 (MQ=255)                                                                                aaCACCTACAGCTGTAATAAGATAAACATAATGTTTTACTCTATTAACACCTACGATGGATGCATAAGAGATGATAACCATAAAGACAATTTGCTTACCAGTATTCAGTATAATTGCAAAGATGAATACAATAAAAACaa                                                       >  2:1110915/1‑140 (MQ=255)                                                                                 acacCTACAGCTGTAATAAGATAAACATAATGTTTTACTCTATTAACACCTACGATGGATGCATAAGAGATGATAACCATAAAGACAATTTGCTTACCAGTATTCAGTATAATTGCAAAGATGAATACAATAAAAACaag                                                      <  1:1254422/140‑1 (MQ=255)                                                                                         aGCTGTAATAAGATAAACATAATGTTTTACTCTATTAACACCTACGATGGATGCATAAGAGATGATAACCATAAAGACAATTTGCTTACCAGTATTCAGTATAATTGCAAAGATGAATACAATAAAAACAagtaaagtaa                                              <  2:664500/140‑1 (MQ=255)                                                                                          gCTGTAATAAGATAAACATAATGTTTTACTCTATTAACACCTACGATGGATGCATAAGAGATGATAACCATAAAGACAATTTGCTTACCAGTATTCAGTATAATTGCAAAGATGAATACAATAAAAACAAgtaaagtaaa                                             >  1:149792/1‑140 (MQ=255)                                                                                          gCTGTAATAAGATAAACATAATGTTTTACTCTATTAACACCTACGATGGATGCATAAGAGATGATAACCATAAAGACAATTTGCTTACCAGCATTCAGTATAATTGCAAAGATGAATACAATAAAAACAAgtaaagtaaa                                             >  1:105299/1‑140 (MQ=255)                                                                                            tGTAATAAGATAAACATAATGTTTTACTCTATTAACACCTACGATGGATGCATAAGAGATGATAACCATAAAGACAATTTGCTTACCAGTATTCAGTATAATTGCAAAGATGAATACAATAAAAACAAGTAAAGTAAATg                                           <  1:1107913/140‑1 (MQ=255)                                                                                               aataaGATAAACATAATGTTTTACTCTATTAACACCTACGATGGATGCATAAGAGATGATAACCATAAAGACAATTTGCTTACCAGTATTCAGTATAATTGCAAAGATGAATACAATAAAAACAAGTAAAGTAAATGttt                                        >  1:640987/1‑140 (MQ=255)                                                                                               aataaGATAAACATAATGTTTTACTCTATTAACACCTACGATGGATGCATAAGAGATGATAACCATAAAGACAATTTGCTTACCAGTATTCAGTATAATTGCAAAGATGAATACAATAAAAACAAGTAAAGTAAATGttt                                        >  2:366470/1‑140 (MQ=255)                                                                                               aataaGATAAACATAATGTTTTACTCTATTAACACCTACGATGGATGCATAAGAGATGATAACCATAAAGACAATTTGCTTACCAGTATTCAGTATAATTGCAAAGATGAATACAATAAAAACAAGTAAAGTAAATGttt                                        >  2:271037/1‑140 (MQ=255)                                                                                                   aGATAAACATAATGTTTTACTCTATTAACACCTACGATGGATGCATAAGAGATGATAACCATAAAGACAATTTGCTTACCAGTATTCAGTATAATTGCAAAGATGAATACAATAAAAACAAGTAAAGTAAATGTTttact                                    <  1:585629/140‑1 (MQ=255)                                                                                                   aGATAAACATAATGTTTTACTCTATTAACACCTACGATGGATGCATAAGAGATGATAACCATAAAGACAATTTGCTTACCAGTATTCAGTATAATTGCAAAGATGAATACAATAAAAACAAGTAAAGTAAATGTTttact                                    <  2:655868/140‑1 (MQ=255)                                                                                                      tAAACATAATGTTTTACTCTATTAACACCTACGATGGATGCATAAGAGATGATAACCATAAAGACAATTTGCTTACCAGTATTCAGTATAATTGCAAAGATGAATACAATAAAAACAAGTAAAGTAAATGTTttacttac                                 >  1:913636/1‑140 (MQ=255)                                                                                                              aTGTTTTACTCTATTAACACCTACGATGGATGCATAAGAGATGATAACCATAAAGACAATTTGCTTACCAGTATTCAGTATAATTGCAAAGATGAATACAATAAAAACAAGTAAAGTAAATGTTTTACTTACCTTTGTAt                         >  2:1962/1‑140 (MQ=255)                                                                                                               tGTTTTACTCTATTAACACCTACGATGGATGCATAAGAGATGATAACCATAAAGACAATTTGCTTACCAGTATTCAGTATAATTGCAAAGATGAATACAATAAAAACAAGTAAAGTAAATGTTTTACTTACCTTTGTAtt                        >  2:1148455/1‑140 (MQ=255)                                                                                                               tGTTTTACTCTATTAACACCTACGATGGATGCATAAGAGATGATAACCATAAAGACAATTTGCTTACCAGTATTCAGTATAATTGCAAAGATGAATACAATAAAAACAAGTAAAGTAAATGTTTTACTTACCTTTGTAtt                        >  2:75106/1‑140 (MQ=255)                                                                                                                   ttACTCTATTAACACCTACGATGGATGCATAAGAGATGATAACCATAAAGACAATTTGCTTACCAGTATTCAGTATAATTGCAAAGATGAATACAATAAAAACAAGTAAAGTAAATGTTTTACTTACCTTTGTATTAGTa                    >  2:104593/1‑140 (MQ=255)                                                                                                                     aCTCTATTAACACCTACGATGGATGCATAAGAGATGATAACCATAAAGACAATTTGCTTACCAGTATTCAGTATAATTGCAAAGATGAATACAATAAAAACAAGTAAAGTAAATGTTTTACTTACCTTTGTATTAGTaaa                  <  1:1109663/140‑1 (MQ=255)                                                                                                                     aCTCTATTAACACCTACGATGGATGCATAAGAGATGATAACCATAAAGACAATTTGCTTACCAGTATTCAGTATAATTGCAAAGATGAATACAATAAAAACAAGTAAAGTAAATGTTTTACTTACCTTTGTATTAGTaaa                  <  1:1089918/140‑1 (MQ=255)                                                                                                                     aCTCTATTAACACCTACGATGGATGCATAAGAGATGATAACCATAAAGACAATTTGCTTACCAGTATTCAGTATAATTGCAAAGATGAATACAATAAAAACAAGTAAAGTAAATGTTTTACTTACCTTTGTATTAGTaaa                  >  2:1089918/1‑140 (MQ=255)                                                                                                                      ctctATTAACACCTACGATGGATGCATAAGAGATGATAACCATAAAGACAATTTGCTTACCAGTATTCAGTATAATTGCAAAGATGAATACAATAAAAACAAGTAAAGTAAATGTTTTACTTACCTTTGTATTAGTAAAt                 >  1:826541/1‑140 (MQ=255)                                                                                                                           ttAACACCTACGATGGATGCATAAGAGATGATAACCATAAAGACAATTTGCTTACCAGTATTCAGTATAATTGCAAAGATGAATACAATAAAAACAAGTAAAGTAAATGTTTTACTTACCTTTGTATTAGTAAAtttttt            >  1:415967/1‑140 (MQ=255)                                                                                                                           ttAACACCTACGATGGATGCATAAGAGATGATAACCATAAAGACAATTTGCTTACCAGTATTCAGTATAATTGCAAAGATGAATACAATAAAAACAAGTAAAGTAAATGTTTTACTTACCTTTGTATTAGTAAAtttttt            >  2:5504/1‑140 (MQ=255)                                                                                                                           ttAACACCTACGATGGATGCATAAGAGATGATAACCATAAAGACAATTTGCTTACCAGTATTCAGTATAATTGCAAAGATGAATACAATAAAAACAAGTAAAGTAAATGTTTTACTTACCTTTGTATTAGTAAAttttt             <  2:510207/139‑1 (MQ=255)                                                                                                                           ttAACACCTACGATGGATGCATAAGAGATGATAACCATAAAGACAATTTGCTTACCAGTATTCAGTATAATTGCAAAGATGAATACAATAAAAACAAGTAAAGTAAATGTTTTACTTACCTTTGTATTAGTAAAttttt             >  1:510207/1‑139 (MQ=255)                                                                                                                             aaCACCTACGATGGATGCATAAGAGATGATAACCATAAAGACAATTTGCTTACCAGTATTCAGTATAATTGCAAAGATGAATACAATAAAAACAAGTAAAGTAAATGTTTTACTTACCTTTGTATTAGTAAATTTTTTAg          <  2:214168/140‑1 (MQ=255)                                                                                                                              acacCTACGATGGATGCATAAGAGATGATAACCATAAAGACAATTTGCTTACCAGTATTCAGTATAATTGCAAAGATGAATACAATAAAAACAAGTAAAGTAAATGTTTTACTTACCTTTGTATTAGTAAATTTTTTAGa         <  1:517750/140‑1 (MQ=255)                                                                                                                                  cTACGATGGATGCATAAGAGATGATAACCATAAAGACAATTTGCTTACCAGTATTCAGTATAATTGCAAAGATGAATACAATAAAAACAAGTAAAGTAAATGTTTTACTTACCTTTGTATTAGTAAATTTTTTAGACCaa     <  1:366470/140‑1 (MQ=255)                                                                                                                                  cTACGATGGATGCATAAGAGATGATAACCATAAAGACAATTTGCTTACCAGTATTCAGTATAATTGCAAAGATGAATACAATAAAAACAAGTAAAGTAAATGTTTTACTTACCTTTGTATTAGTAAATTTTTTAGACCaa     <  1:271037/140‑1 (MQ=255)                                                                                                                                  cTACGATGGATGCATAAGAGATGATAACCATAAAGACAATTTGCTTACCAGTATTCAGTATAATTGCAAAGATGAATACAATAAAAACAAGTAAAGTAAATGTTTTACTTACCTTTGTATTAGTAAATTTTTTAGACCaa     <  2:585158/140‑1 (MQ=255)                                                                                                                                  cTACGATGGATGCATAAGAGATGATAACCATAAAGACAATTTGCTTACCAGTATTCAGTATAATTGCAAAGATGAATACAATAAAAACAAGTAAAGTAAATGTTTTACTTACCTTTGTATTAGTAAATTTTTTAGACCaa     <  1:75106/140‑1 (MQ=255)                                                                                                                                   tACGATGGATGCATAAGAGATGATAACCATAAAGACAATTTGCTTACCAGTATTCAGTATAATTGCAAAGATGAATACAATAAAAACAAGTAAAGTAAATGTTTTACTTACCTTTGTATTAGTAAATTTTTTAGACCaaa    >  2:1187285/1‑140 (MQ=255)                                                                                                                                   tACGATGGATGCATAAGAGATGATAACCATAAAGACAATTTGCTTACCAGTATTCAGTATAATTGCAAAGATGAATACAATAAAAACAAGTAAAGTAAATGTTTTACTTACCTTTGTATTAGTAAATTTTTTAGACCaaa    >  2:1015748/1‑140 (MQ=255)                                                                                                                                   tACGATGGATGCATAAGAGATGATAACCATAAAGACAATTTGCTTACCAGTATTCAGTATAATTGCAAAGATGAATACAATAAAAACAAGTAAAGTAAATGTTTTACTTACCTTTGTATTAGTAAATTTTTTAGACCaaa    >  2:644823/1‑140 (MQ=255)                                                                                                                                     cGATGGATGCATAAGAGATGATAACCATAAAGACAATTTGCTTACCAGTATTCAGTATAATTGCAAAGATGAATACAATAAAAACAAGTAAAGTAAATGTTTTACTTACCTTTGTATTAGTAAATTTTTTAGAc        <  2:66412/134‑1 (MQ=255)                                                                                                                                     cGATGGATGCATAAGAGATGATAACCATAAAGACAATTTGCTTACCAGTATTCAGTATAATTGCAAAGATGAATACAATAAAAACAAGTAAAGTAAATGTTTTACTTACCTTTGTATTAGTAAATTTTTTAGAc        >  1:66412/1‑134 (MQ=255)                                                                                                                                     cGATGGATGCATAAGAGATGATAACCATAAAGACAATTTGCTTACCAGTATTCAGTATAATTGCAAAGATGAATACAATAAAAACAAGTAAAGTAAATGTTTTACTTACCTTTGTATTAGTAAATTTTTTAGACCaaata  >  1:1317932/1‑140 (MQ=255)                                                                                                                                     cGATGGATGCATAAGAGATGATAACCATAAAGACAATTTGCTTACCAGTATTCAGTATAATTGCAAAGATGAATACAATAAAAACAAGTAAAGTAAATGTTTTACTTACCTTTGTATTAGTAAATTTTTTAGACCaaata  >  1:743066/1‑140 (MQ=255)                                                                                                                                     cGATGGATGCATAAGAGATGATAACCATAAAGACAATTTGCTTACCAGTATTCAGTATAATTGCAAAGATGAATACAATAAAAACAAGTAAAGTAAATGTTTTACTTACCTTTGTATTAGTAAATTTTTTAGACCaaata  >  1:264555/1‑140 (MQ=255)                                                                                                                                     cGATGGATGCATAAGAGATGATAACCATAAAGACAATTTGCTTACCAGTATTCAGTATAATTGCAAAGATGAATACAATAAAAACAAGTAAAGTAAATGTTTTACTTACCTTTGTATTAGTAAATTTTTTAGACCaaata  >  2:515961/1‑140 (MQ=255)                                                                                                                                          |                                                                                                                                       GGCTGACCAAATACATGGATAGATAATATGCCATCCCCCCAGGCAGTCCACGTAAAAAGAGCATATACAAGGAGAATAGAACACCTACAGCTGTAATAAGATAAACATAATGTTTTACTCTATTAACACCTACGATGAATGCATAAGAGATGATAACCATAAAGACAATTTGCTTACCAGTATTCAGTATAATTGCAAAGATGAATACAATAAAAACAAGTAAAGTAAATGTTTTACTTACCTTTGTATTAGTAAATTTTTTAGACCAAATA  >  NC_000913/2106479‑2106750

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑