breseq  version 0.35.4  revision f352f80f4bc9
mutation predictions | marginal predictions | summary statistics | genome diff | command line log

Predicted mutations
evidence position mutation annotation gene description
MC JC 257,908 Δ776 bp insB9[crl] insB9, insA9, [crl]
JC JC 570,591 IS5 (+) +4 bp intergenic (+163/‑299) ybcK → / → ybcL DLP12 prophage; putative recombinase/DLP12 prophage; periplasmic protein YbcL
RA 670,604 T→G K334N (AAA→AAC holA ← DNA polymerase III subunit delta
RA 781,291 +A intergenic (+69/‑78) lysY → / → lysZ tRNA‑Lys/tRNA‑Lys
RA 889,923 A→G intergenic (‑104/‑166) ybjL ← / → ybjM putative transport protein YbjL/putative inner membrane protein
RA 1,196,220 C→T H366H (CAC→CAT icd → isocitrate dehydrogenase
RA 1,196,232 C→T T370T (ACC→ACT icd → isocitrate dehydrogenase
JC JC 1,879,829 Δ1 bp :: IS186 (–) +6 bp :: Δ1 bp coding (115‑120/360 nt) yeaR ← DUF1971 domain‑containing protein YeaR
MC JC 1,978,503 Δ776 bp insB‑5insA‑5 insB‑5, insA‑5
RA 2,173,363 Δ2 bp pseudogene (915‑916/1358 nt) gatC ← galactitol‑specific PTS enzyme IIC component
RA 2,280,436 A→C A16A (GCT→GCG rsuA ← 16S rRNA pseudouridine(516) synthase
RA 2,731,550 T→C intergenic (‑393/+50) rrsG ← / ← clpB 16S ribosomal RNA/ClpB chaperone
RA 3,354,487 C→T intergenic (‑437/‑238) yhcC ← / → gltB radical SAM family oxidoreductase YhcC/glutamate synthase subunit GltB
RA 3,560,455 +G pseudogene (151/758 nt) glpR ← DNA‑binding transcriptional repressor GlpR
JC JC 3,727,211 IS5 (–) +4 bp coding (148‑151/438 nt) yiaA ← conserved inner membrane protein YiaA
RA 3,815,883 +C coding (667/687 nt) rph ← truncated RNase PH
RA 4,296,381 +GC intergenic (+587/+55) gltP → / ← yjcO glutamate/aspartate : H(+) symporter GltP/Sel1 repeat‑containing protein YjcO
RA 4,435,778 G→A P263L (CCA→CTA)  cpdB ← 2'3' cyclic nucleotide phosphodiesterase/3' nucleotidase

Unassigned missing coverage evidence
   seq id start end size ←reads reads→ gene description
* * ÷ NC_000913 781292 781536 245 42 [38] [39] 40 lysZ lysZ
* * ÷ NC_000913 1196245 1211436 15192 45 [35] [38] 40 [icd]–[icdC] [icd],ymfD,ymfE,lit,intE,xisE,ymfH,ymfI,ymfJ,ymfK,ymfT,ymfL,ymfM,oweE,ymfN,aaaE,ymfR,beeE,jayE,ymfQ,ycfK,tfaP,tfaE,stfE,pinE,mcrA,[icdC]
* * ÷ NC_000913 2066301–2066464 2066637–2066466 3–337 41 [38] [37] 40 [insH‑6] [insH‑6]
* * ÷ NC_000913 3423760–3424233 3424528–3424238 6–769 41 [39] [36] 40 [rrfD]–[rrlD] [rrfD],[rrlD]

Unassigned new junction evidence
  seq id position reads (cov) reads (cov) score skew freq annotation gene product
* ? NC_000913 = 12994980 (0.000)177 (1.260) 87/266 0.1 100% intergenic (+253/‑33) ychE/insH21 putative inner membrane protein/IS5 transposase and trans‑activator
?NC_000913 1300698 = 0 (0.000)intergenic (+151/‑484) insH21/oppA IS5 transposase and trans‑activator/oligopeptide ABC transporter periplasmic binding protein
* ? NC_000913 3363001 =221 (1.530)259 (1.800) 83/272 0.2 70.2% coding (195/2382 nt) yhcD putative fimbrial usher protein YhcD
?NC_000913 3766087 = 0 (0.000)coding (3905/4134 nt) rhsA rhs element protein RhsA
* ? NC_000913 = 3653230NA (NA)131 (0.900) 68/274 0.5 94.2% noncoding (1/1195 nt) IS5 repeat region
?NC_000913 = 3766089 8 (0.060)coding (3907/4134 nt) rhsA rhs element protein RhsA