Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	626,941	T→A	I291N (ATC→AAC)	entE →	2,3‑dihydroxybenzoate‑AMP ligase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	626,941	0	T	A	100.0%	232.4 / NA	67	I291N (ATC→AAC)	entE	2,3‑dihydroxybenzoate‑AMP ligase
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base A (40/27);  total (40/27)

TTCTTGCCGGAGGAACGGTTGTTCTGGCGGCCGATCCCAGCGCCACGCTCTGTTTCCCATTGATTGAAAAACATCAGGTTAACGTTACCGCGCTGGTGCCACCCGCAGTCAGCCTGTGGTTGCAGGCGCTGATCGAAGGCGAAAGCCGGGCGCAGCTTGCCTCGCTGAAACTGTTACAGGTCGGCGGCGCACGTCTTTCTGCCACCCTTGCGGCGCGTATTCCCGCTGAGATTGGCTGTCAGTTGCAGCAGGTGTTTGGCATGGCGGA  >  NC_000913/626809‑627076                                                                                                                                     |                                                                                                                                        ttcttGCCGGAGGAACGGTTGTTCTGGCGGCCGATCCCAGCGCCACGCTCTGTTTCCCATTGATTGAAAAACATCAGGTTAACGTTACCGCGCTGGTGCCACCCGCAGTCAGCCTGTGGTTGCAGGCGCTGAACGAAGGc                                                                                                                                  >  1:144241/1‑140 (MQ=255)         ggaggaACGGTTGTTCTGGCGGCCGATCCCAGCGCCACGCTCTGTTTCCCATTGATTGAAAAACATCAGGTTAACGTTACCGCGCTGGTGCCACCCGCAGTCAGCCTGTGGTTGCAGGCGCTGAACGAAGGCGAAAGCCg                                                                                                                          >  1:792549/1‑140 (MQ=255)          gaggaACGGTTGTTCTGGCGGCCGATCCCAGCGCCACGCTCTGTTTCCCATTGATTGAAAAACATCAGGTTAACGTTACCGCGCTGGTGCCACCCGCAGTCAGCCTGTGGTTGCAGGCGCTGAACGAAGGCGAAAGCCgg                                                                                                                         >  2:758665/1‑140 (MQ=255)           aggaACGGTTGTTCTGGCGGCCGATCCCAGCGCCACGCTCTGTTTCCCATTGATTGAAAAACATCAGGTTAACGTTACCGCGC‑‑‑TGCCACCCGCAGTCAGCCTGTGGTTGCAGGCGCTGAACGAAGGCGAAAGCCGGgcgc                                                                                                                     >  2:11385/1‑140 (MQ=255)            ggaACGGTTGTTCTGGCGGCCGATCCCAGCGCCACGCTCTGTTTCCCATTGATTGAAAAACATCAGGTTAACGTTACCGCGCTGGTGCCACCCGCAGTCAGCCTGTGGTTGCAGGCGCTGAACGAAGGCGa                                                                                                                                >  2:44577/1‑131 (MQ=255)            ggaACGGTTGTTCTGGCGGCCGATCCCAGCGCCACGCTCTGTTTCCCATTGATTGAAAAACATCAGGTTAACGTTACCGCGCTGGTGCCACCCGCAGTCAGCCTGTGGTTGCAGGCGCTGAACGAAGGCGa                                                                                                                                <  1:44577/131‑1 (MQ=255)                       tCTGGCGGCCGATCCCAGCGCCACGCTCTGTTTCCCATTGATTGAAAAACATCAGGTTAACGTTACCGCGCTGGTGCCACCCGCAGTCAGCCTGTGGTTGCAGGCGCTGAACGAAGGCGAAAGCCGGGCGCAGCTTGCCt                                                                                                            >  2:124277/1‑140 (MQ=255)                       tCTGGCGGCCGATCCCAGCGCCACGCTCTGTTTCCCATTGATTGAAAAACATCAGGTTAACGTTACCGCGCTGGTGCCACCCGCAGTCAGCCTGTGGTTGCAGGCGCTGAACGAAGGCGAAAGCCGGGCGCAGCTTGCCt                                                                                                            >  1:94452/1‑140 (MQ=255)                       tCTGGCGGCCGATCCCAGCGCCACGCTCTGTTTCCCATTGATTGAAAAACATCAGGTTAACGTTACCGCGCTGGTGCCACCCGCAGTCAGCCTGTGGTTGCAGGCGCTGAACGAAGGCGAAAGCCGGGCGCAGCTTGCCt                                                                                                            >  2:81310/1‑140 (MQ=255)                             ggcCGATCCCAGCGCCACGCTCTGTTTCCCATTGATTGAAAAACATCAGGTTAACGTTACCGCGCTGGTGCCACCCGCAGTCAGCCTGTGGTTGCAGGCGCTGAACGAAGGCGAAAGCCGGGCGCAGCTTGCCTCGCTGa                                                                                                      <  2:782023/140‑1 (MQ=255)                                     ccAGCGCCACGCTCTGTTTCCCATTGATTGAAAAACATCAGGTTAACGTTACCGCGCTGGTGCCACCCGCAGTCAGCCTGTGGTTGCAGGCGCTGAACGAAGGCGAAAGCCGGGCGCAGCTTGCCTCGCTGAAACTGTTa                                                                                              <  1:124277/140‑1 (MQ=255)                                       aGCGCCACGCTCTGTTTCCCATTGATTGAAAAACATCAGGTTAACGTTACCGCGC‑‑‑TGCCACCCGCAGTCAGCCTGTGGTTGCAGGCGCTGAACGAAGGCGAAAGCCGGGCGCAGCTTGCCTCGCTGAAACTGTTACAGGt                                                                                         <  1:11385/140‑1 (MQ=255)                                         cgcCACGCTCTGTTTCCCATTGATTGAAAAACATCAGGTTAACGTTACCGCGCTGGTGCCACCCGCAGTCAGCCTGTGGTTGCAGGCGCTGAACGAAGGCGAAAGCCGGGCGCAGCTTGCCTCGCTGAAACTGTTACAgg                                                                                          >  1:53221/1‑140 (MQ=255)                                            cACGCTCTGTTTCCCATTGATTGAAAAACATCAGGTTAACGTTACCGCGCTGGTGCCACCCGCAGTCAGCCTGTGGTTGCAGGCGCTGAACGAAGGCGAAAGCCGGGCGCAGCTTGCCTCGCTGAAACTGTTACAGGTcg                                                                                       >  2:826389/1‑140 (MQ=255)                                              cGCTCTGTTTCCCATTGATTGAAAAACATCAGGTTAACGTTACCGCGCTGGTGCCACCCGCAGTCAGCCTGTGGTTGCAGGCGCTGAACGAAGGCGAAAGCCGGGCGCAGCTTGCCTCGCTGAAACTGTTACAGGTcggc                                                                                     >  2:69146/1‑140 (MQ=255)                                              cGCTCTGTTTCCCATTGATTGAAAAACATCAGGTTAACGTTACCGCGCTGGTGCCACCCGCAGTCAGCCTGTGGTTGCAGGCGCTGAACGAAGGCGAAAGCCGGGCGCAGCTTGCCTCGCTGAAACTGTTACAGGTcggc                                                                                     >  1:818683/1‑140 (MQ=255)                                              cGCTCTGTTTCCCATTGATTGAAAAACATCAGGTTAACGTTACCGCGCTGGTGCCACCCGCAGTCAGCCTGTGGTTGCAGGCGCTGAACGAAGGCGAAAGCCGGGCGCAGCTTGCCTCGCTGAAACTGTTACAGGTcggc                                                                                     >  1:1199245/1‑140 (MQ=255)                                                  ctGTTTCCCATTGATTGAAAAACATCAGGTTAACGTTACCGCGCTGGTGCCACCCGCAGTCAGCCTGTGGTTGCAGGCGCTGAACGAAGGCGAAAGCCGGGCGCAGCTTGCCTCGCTGAAACTGTTACAGGTCGgcggcg                                                                                 <  2:144241/140‑1 (MQ=255)                                                   tGTTTCCCATTGATTGAAAAACATCAGGTTAACGTTACCGCGCTGGTGCCACCCGCAGTCAGCCTGTGGTTGCAGGCGCTGAACGAAGGCGAAAGCCGGGCGCAGCTTGCCTCGCTGAAACTGTTACAGGTCGGCGgcgc                                                                                >  1:36986/1‑140 (MQ=255)                                                        cccATTGATTGAAAAACATCAGGTTAACGTTACCGCGCTGGTGCCACCCGCAGTCAGCCTGTGGTTGCAGGCGCTGAACGAAGGCGAAAGCCGGGCGCAGCTTGCCCCGCTGAAACTGTTACAGGTCGGCGGCGCACGTc                                                                           <  2:215039/140‑1 (MQ=255)                                                          cATTGATTGAAAAACATCAGGTTAACGTTACCGCGCTGGTGCCACCCGCAGTCAGCCTGTGGTTGCAGGCGCTGAACGAAGGCGAAAGCCGGGCGCAGCTTGCCTCGCTGAAACTGTTACAGGTCGGCGGCGCACGTCtt                                                                         >  2:954061/1‑140 (MQ=255)                                                             tgattgaAAAACATCAGGTTAACGTTACCGCGCTGGTGCCACCCGCAGTCAGCCTGTGGTTGCAGGCGCTGAACGAAGGCGAAAGCCGGGCGCAGCTTGCCTCGCTGAAACTGTTACAGGTCGGCGGCGCACGTCTTTCt                                                                      >  1:886115/1‑140 (MQ=255)                                                             tgattgaAAAACATCAGGTTAACGTTACCGCGCTGGTGCCACCCGCAGTCAGCCTGTGGTTGCAGGCGCTGAACGAAGGCGAAAGCCGGGCGCAGCTTGCCTCGCTGAAACTGTTACAGGTCGGCGGCGCACGTCTTTCt                                                                      >  2:1255518/1‑140 (MQ=255)                                                               attgaAAAACATCAGGTTAACGTTACCGCGCTGGTGCCACCCGCAGTCAGCCTGTGGTTGCAGGCGCTGAACGAAGGCGAAAGCCGGGCGCAGCTTGCCTCGCTGAAACTGTTACAGGTCGGCGGCGCa                                                                               <  2:565387/129‑1 (MQ=255)                                                               attgaAAAACATCAGGTTAACGTTACCGCGCTGGTGCCACCCGCAGTCAGCCTGTGGTTGCAGGCGCTGAACGAAGGCGAAAGCCGGGCGCAGCTTGCCTCGCTGAAACTGTTACAGGTCGGCGGCGCa                                                                               >  1:565387/1‑129 (MQ=255)                                                               attgaAAAACATCAGGTTAACGTTACCGCGCTGGTGCCACCCGCAGTCAGCCTGTGGTTGCAGGCGCTGAACGAAGGCGAAAGCCGGGCGCAGCTTGCCTCGCTGAAACTGTTACAGGTCGGCGGCGCACGTCTTTCTGc                                                                    <  2:886115/140‑1 (MQ=255)                                                               attgaAAAACATCAGGTTAACGTTACCGCGCTGGTGCCACCCGCAGTCAGCCTGTGGTTGCAGGCGCTGAACGAAGGCGAAAGCCGGGCGCAGCTTGCCTCGCTGAAACTGTTACAGGTCGGCGGCGCACGTCTTTCTGc                                                                    <  1:758665/140‑1 (MQ=255)                                                               attgaAAAACATCAGGTTAACGTTACCGCGCTGGTGCCACCCGCAGTCAGCCTGTGGTTGCAGGCGCTGAACGAAGGCGAAAGCCGGGCGCAGCTTGCCTCGCTGAAACTGTTACAGGTCGGCGGCGCACGTCTTTCTGc                                                                    >  2:504824/1‑140 (MQ=255)                                                               attgaAAAACATCAGGTTAACGCTACCGCGCTGGTGCCACCCGCAGTCAGCCTGTGGTTGCAGGCGCTGAACGAAGGCGAAAGCCGGGCGCAGCTTGCCTCGCTGAAACTGTTACA‑GTCGGCGGCGCACGTCTTTCTGcc                                                                   >  1:594065/1‑140 (MQ=255)                                                                 tgaAAAACATCAGGTTAACGTTACCGCGCTGGTGCCACCCGCAGTCAGCCTGTGGTTGCAGGCGCTGAACGAAGGCGAAAGCCGGGCGCAGCTTGCCTCGCTGAAACTGTTACAGGTCGGCGGCGCACGTCTTTCTGCCa                                                                  >  1:1108065/1‑140 (MQ=255)                                                                      aaCATCAGGTTAACGTTACCGCGCTGGTGCCACCCGCAGTCAGCCTGTGGTTGCAGGCGCTGAACGAAGGCGAAAGCCGGGCGCAGCTTGCCTCGCTGAAACTGTTACAGGTCGGCGGCGCACGTCTTTCTGCCACCCtt                                                             <  2:467965/140‑1 (MQ=255)                                                                         aTCAGGTTAACGTTACCGCGCTGGTGCCACCCGCAGTCAGCCTGTGGTTGCAGGCGCTGAACGAAGGCGAAAGCCGGGCGCAGCTTGCCTCGCTGAAACTGTTACAGGTCGGCGGCGCACGTCTTTCTGCCCCCCTTgcg                                                          <  2:53221/140‑1 (MQ=255)                                                                         aTCAGGTTAACGTTACCGCGCTGGTGCCACCCGCAGTCAGCCTGTGGTTGCAGGCGCTGAACGAAGGCGAAAGCCGGGCGCAGCTTGCCTCGCTGAAACTGTTACAGGTCGGCGGCGCACGTCTTTCTGCCACCCTTgcg                                                          <  2:792549/140‑1 (MQ=255)                                                                         aTCAGGTTAACGTTACCGCGCTGGTGCCACCCGCAGTCAGCCTGTGGTTGCAGGCGCTGAACGAAGGCGAAAGCCGGGCGCAGCTTGCCTCGCTGAAACTGTTACAGGTCGGCGGCGCACGTCTTTCTGCCACCCTTgcg                                                          <  2:1199245/140‑1 (MQ=255)                                                                             ggTTAACGTTACCGCGCTGGTGCCACCCGCAGTCAGCCTGTGGTTGCAGGCGCTGAACGAAGGCGAAAGCCGGGCGCAGCTTGCCTCGCTGAAACTGTTACAGGTCGGCGGCGCACGTCTTTCTGCCACCCTTgcg                                                          <  2:995769/136‑1 (MQ=255)                                                                             ggTTAACGTTACCGCGCTGGTGCCACCCGCAGTCAGCCTGTGGTTGCAGGCGCTGAACGAAGGCGAAAGCCGGGCGCAGCTTGCCTCGCTGAAACTGTTACAGGTCGGCGGCGCACGTCTTTCTGCCACCCTTgcg                                                          >  1:995769/1‑136 (MQ=255)                                                                              gTTAACGTTACCGCGCTGGTGCCACCCGCAGTCAGCCTGTGGTTGCAGGCGCTGAACGAAGGCGAAAGCCGGGCGCAGCTTGCCTCGCTGAAACTGTTACAGGTCGGCGGCGCACGTCTTTCTGCCACCCTTGCGGcgcg                                                     >  1:687677/1‑140 (MQ=255)                                                                                 aaCGTTACCGCGCTGGTGCCACCCGCAGTCAGCCTGTGGTTGCAGGCGCTGAACGAAGGCGAAAGCCGGGCGCAGCTTGCCTCGCTGAAACTGTTACAGGTCGGCGGCGCACGTCTTTCTGCCACCCTTGCGGCGCGTAt                                                  <  1:1255518/140‑1 (MQ=255)                                                                                  aCGTTACCGCGCTGGTGCCACCCGCAGTCAGCCTGTGGTTGCAGGCGCTGAACGAAGGCGAAAGCCGGGCGCAGCTTGCCTCGCTGAAACTGTTACAGGTCGGCGGCGCACGTCTTTCTGCCACCCTTGCGGCGCGTAtt                                                 >  2:1249134/1‑140 (MQ=255)                                                                                        ccGCGCTGGTGCCACCCGCAGTCAGCCTGTGGTTGCAGGCGCTGAACGAAGGCGAAAGCCGGGCGCAGCTTGCCTCGCTGAAACTGTTACAGGTCGGCGGCGCACGTCTTTCTGCCACCCTTGCGGCGCGTATTCCCGCt                                           <  1:69146/140‑1 (MQ=255)                                                                                            gcTGGTGCCACCCGCAGTCAGCCTGTGGTTGCAGGCGCTGAACGAAGGCGAAAGCCGGGCGCAGCTTGCCTCGCTGAAACTGTTACAGGTCGGCGGCGCACGTCTTTCTGCAACCCTTGCGGCGCGTATTCCCGCTgaga                                       >  1:1383872/1‑140 (MQ=255)                                                                                             cTGGTGCCACCCGCAGTCAGCCTGTGGTTGCAGGCGCTGAACGAAGGCGAAAGCCGGGCGCAGCTTGCCTCGCTGAAACTGTTACAGGTCGGCGGCGCACGTCTTTCTGCCACCCTTGCGGCGCGTATTCCCGCTGAGAt                                      >  2:17819/1‑140 (MQ=255)                                                                                               ggTGCCACCCGCAGTCAGCCTGTGGTTGCAGGCGCTGAACGAAGGCGAAAGCCGGGCGCAGCTTGCCTCGCTGAAACTGTTACA‑GTCGGCGGCGCACGTCTTTCTGCCACCCTTGCGGCGCGTATTCCCGCTGAGATTgg                                   <  2:594065/140‑1 (MQ=255)                                                                                                gTGCCACCCGCAGTCAGCCTGTGGTTGCAGGCGCTGAACGAAGGCGAAAGCCGGGCGCAGCTTGCCTCGCTGAAACTGTTACAGGTCGGCGGCGCACGTCTTTCTGCCACCCTTGCGGCGCGTATTCCCGCt                                           >  1:1030135/1‑132 (MQ=255)                                                                                                gTGCCACCCGCAGTCAGCCTGTGGTTGCAGGCGCTGAACGAAGGCGAAAGCCGGGCGCAGCTTGCCTCGCTGAAACTGTTACAGGTCGGCGGCGCACGTCTTTCTGCCACCCTTGCGGCGCGTATTCCCGCt                                           <  2:1030135/132‑1 (MQ=255)                                                                                                        cGCAGTCAGCCTGTGGTTGCAGGCGCTGAACGAAGGCGAAAGCCGGGCGCAGCTTGCCTCGCTGAAACTGTTACAGGTCGGCGGCGCACGTCTTTCTGCCACCCTTGCGGCGCGTATTCCCGCTGAGATTGGCTGTCAGt                           <  1:826389/140‑1 (MQ=255)                                                                                                        cGCAGTCAGCCTGTGGTTGCAGGCGCTGAACGAAGGCGAAAGCCGGGCGCAGCTTGCCTCGCTGAAACTGTTACAGGTCGGCGGCGCACGTCTTTCTGCCACCCTTGCGGCGCGTATTCCCGCTGAGATTGGCTGTCAGt                           <  2:257614/140‑1 (MQ=255)                                                                                                          cAGTCAGCCTGTGGTTGCAGGCGCTGAACGAAGGCGAAAGCCGGGCGCAGCTTGCCTCGCTGAAACTGTTACAGGTCGGCGGCGCACGTCTTTCTGCCACCCTTGCGGCGCGTATTCCCGCTGAGATTGGCTGTCAGTTg                         >  2:1238059/1‑140 (MQ=255)                                                                                                          cAGTCAGCCTGTGGTTGCAGGCGCTGAACGAAGGCGAAAGCCGGGCGCAGCTTGCCTCGCTGAAACTGTTACAGGTCGGCGGCGCACGTCTTTCTGCCACCCTTGCGGCGCGTATTCCCGCTGAGATTGGCTGTCAGTTg                         <  2:36986/140‑1 (MQ=255)                                                                                                          cAGTCAGCCTGTGGTTGCAGGCGCTGAACGAAGGCGAAAGCCGGGCGCAGCTTGCCTCGCTGAAACTGTTACAGGTCGGCGGCGCACGTCTTTCTGCCACCCTTGCGGCGCGTATTCCCGCTGAGATTGGCTGTCAGTTg                         >  1:474402/1‑140 (MQ=255)                                                                                                           aGTCAGCCTGTGGTTGCAGGCGCTGAACGAAGGCGAAAGCCGGGCGCAGCTTGCCTCGCTGAAACTGTTACAGGTCGGCGGCGCACGTCTTTCTGCCACCCTTGCGGCGCGTATTCCCGCTGAGATTGGCTGt                               <  1:151005/133‑1 (MQ=255)                                                                                                           aGTCAGCCTGTGGTTGCAGGCGCTGAACGAAGGCGAAAGCCGGGCGCAGCTTGCCTCGCTGAAACTGTTACAGGTCGGCGGCGCACGTCTTTCTGCCACCCTTGCGGCGCGTATTCCCGCTGAGATTGGCTGt                               >  2:151005/1‑133 (MQ=255)                                                                                                              cAGCCTGTGGTTGCAGGCGCTGAACGAAGGCGAAAGCCGGGCGCAGCTTGCCTCGCTGAAACTGTTACAGGTCGGCGGCGCACGTCTTTCTGCCACCCTTGCGGCGCGTATTCCCGCTGAGATTGGCTGTCAGTTgcagc                     <  1:72966/140‑1 (MQ=255)                                                                                                              cAGCCTGTGGTTGCAGGCGCTGAACGAAGGCGAAAGCCGGGCGCAGCTTGCCTCGCTGAAACTGTTACAGGTCGGCGGCGCACGTCTTTCTGCCACCCTTGCGGCGCGTATTCCCGCTGAGATTGGCTGTCAGTTgcagc                     <  1:249350/140‑1 (MQ=255)                                                                                                              cAGCCTGTGGTTGCAGGCGCTGAACGAAGGCGAAAGCCGGGCGCAGCTTGCCTCGCTGAAACTGTTACAGGTCGGCGGCGCACGTCTTTCTGCCACCCTTGCGGCGCGTATTCCCGCTGAGATTGGCTGTCAGTTgcagc                     <  2:77368/140‑1 (MQ=255)                                                                                                               aGCCTGTGGTTGCAGGCGCTGAACGAAGGCGAAAGCCGGGCGCAGCTTGCCTCGCTGAAACTGTTACAGGTCGGCGGCGCACGTCTTTCTGCCACCCTTGCGGCGCGTATTCCCGCTGAGATTGGCTGTCAGTTgcagca                    >  2:562089/1‑140 (MQ=255)                                                                                                                gCCTGTGGTTGCAGGCGCTGAACGAAGGCGAAAGCCGGGCGCAGCTTGCCTCGCTGAAACTGTTACAGGTCGGCGGCGCACGTCTTTCTGCCACCCTTGCGGCGCGTATTCCCGCTGAGATTGGCTGTCAGTTGcagcag                   >  2:303154/1‑140 (MQ=255)                                                                                                                 ccTGTGGTTGCAGGCGCTGAACGAAGGCGAAAGCCGGGCGCAGCTTGCCTCGCTGAAACTGTTACAGGTCGGCGGCGCACGTCTTTCTGCCACCCTTGCGGCGCGTATTCCCGCTGAGATTGGCTGTCAGTTGCAGCAgg                  >  2:196136/1‑140 (MQ=255)                                                                                                                 ccTGTGGTTGCAGGCGCTGAACGAAGGCGAAAGCCGGGCGCAGCTTGCCTCGCTGAAACTGTTACAGGTCGGCGGCGCACGTCTTTCTGCCACCCTTGCGGCGCGTATTCCCGCTGAGATTGGCTGTCAGTTGCAGCAgg                  >  2:644155/1‑140 (MQ=255)                                                                                                                 ccTGTGGTTGCAGGCGCTGAACGAAGGCGAAAGCCGGGCGCAGCTTGCCTCGCTGAAACTGTTACAGGTCGGCGGCGCACGTCTTTCTGCCACCCTTGCGGCGCGTATTCCCGCTGAGATTGGCTGTCAGTTGCAGCAgg                  >  2:53366/1‑140 (MQ=255)                                                                                                                     tgGTTGCAGGCGCTGAACGAAGGCGAAAGCCGGGCGCAGCTTGCCTCGCTGAAACTGTTACAGGTCGGCGGCGCACGTCTTTCTGCCACCCTTGCGGCGCGTATTCCCGCTGAGATTGGCTGTCAGTTGCAGCAGGTGtt              >  1:1106967/1‑140 (MQ=255)                                                                                                                       gTTGCAGGCGCTGAACGAAGGCGAAAGCCGGGCGCAGCTTGCCTCGCTGAAACTGTTACAGGTCGGCGGCGCACGTCTTTCTGCCACCCTTGCGGCGCGTATTCCCGCTGAGATTGGCTGTCAGTTGCAGCAGGTGTTTg            >  1:7637/1‑140 (MQ=255)                                                                                                                           cAGGCGCTGAACGAAGGCGAAAGCCGGGCGCAGCTTGCCTCGCTGAAACTGTTACAGGTCGGCGGCGCACGTCTTTCTGCCACCCATGCGGCGCGTATTCCCGCTGAGATTGGCTGTCAGTTGCAGCAGGTGTTTGGCAt        >  1:1202221/1‑140 (MQ=255)                                                                                                                               cgcTGAACGAAGGCGAAAGCCGGGCGCAGCTTGCCTCGCTGAAACTGTTACAGGTCGGCGGCGCACGTCTTTCTGCCACCCTTGCGGCGCGTATTCCCGCTGAGATTGGCTGTCAGTTgcag                      >  2:388799/1‑122 (MQ=255)                                                                                                                               cgcTGAACGAAGGCGAAAGCCGGGCGCAGCTTGCCTCGCTGAAACTGTTACAGGTCGGCGGCGCACGTCTTTCTGCCACCCTTGCGGCGCGTATTCCCGCTGAGATTGGCTGTCAGTTgcag                      <  1:388799/122‑1 (MQ=255)                                                                                                                               cgcTGAACGAAGGCGAAAGCCGGGCGCAGCTTGCCTCGCTGAAACTGTTACAGGTCGGCGGCGCACGTCTTTCTGCCACCCTTGCGGCGCGTATTCCCGCTGAGATTGGCTGTCAGTTGCAGCAGgtgt               >  1:255838/1‑129 (MQ=255)                                                                                                                               cgcTGAACGAAGGCGAAAGCCGGGCGCAGCTTGCCTCGCTGAAACTGTTACAGGTCGGCGGCGCACGTCTTTCTGCCACCCTTGCGGCGCGTATTCCCGCTGAGATTGGCTGTCAGTTGCAGCAGgtgt               <  2:255838/129‑1 (MQ=255)                                                                                                                                 cTGAACGAAGGCGAAAGCCGGGCGCAGCTTGCCTCGCTGAAACTGTTACAGGTCGGCGGCGCACGTCTTTCTGCCACCCTTGCGGCGCGTATTCCCGCTGAGATTGGCTGTCAGTTGCAGCAGGTGTTTGGCATGGCGGa  <  2:7637/140‑1 (MQ=255)                                                                                                                                     |                                                                                                                                        TTCTTGCCGGAGGAACGGTTGTTCTGGCGGCCGATCCCAGCGCCACGCTCTGTTTCCCATTGATTGAAAAACATCAGGTTAACGTTACCGCGCTGGTGCCACCCGCAGTCAGCCTGTGGTTGCAGGCGCTGATCGAAGGCGAAAGCCGGGCGCAGCTTGCCTCGCTGAAACTGTTACAGGTCGGCGGCGCACGTCTTTCTGCCACCCTTGCGGCGCGTATTCCCGCTGAGATTGGCTGTCAGTTGCAGCAGGTGTTTGGCATGGCGGA  >  NC_000913/626809‑627076

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑