Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	3,556,205	0	A	C	25.0%	169.9 / 23.4	76	S215R (AGT→AGG) ‡	rtcA	RNA 3'‑terminal phosphate cyclase
Reads supporting (aligned to +/- strand):  ref base A (55/2);  new base C (0/19);  total (55/21)
Fisher's exact test for biased strand distribution p-value = 5.49e-16
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00
Rejected as consensus: Frequency below/above cutoff threshold.

CTCGGCACTGACGCGCTTTTCACCGACGACAAAAAAGCGTTCGGTGATATTTTCACTTTCGACTTCAAGC‑GAGACGGTATTACCCGGCCCCTGGTC‑GCGCGGCAGGTTATGAATATTCTGTTCATGCAGGGAAAAACTCCCCGCCAGTGTAGCGATTTCACGCTCAGCAACATGGCGCGGCACACCAGCTAATAGAACTTCTCCACGCATCTGCACAATGTTCCCGCGCTCGCCAAGTTGCAAGGTGTTAAACGATGCCACGGGCGAGACTTCC  >  NC_000913/3556070‑3556343                                                                                                                                          |                                                                                                                                           cTCGGCACTGACGCGCTTTTCACCGACGACAAAAAAGCGTTCGGTGATATTTTCACTTTCGACTTCAAGC‑GAGACGGTATTACCCGGCCCCTGGTC‑GCGCGGCAGGTTATGAATATTCTGTTCATGCAGGGAAAAACTcc                                                                                                                                        >  1:1915191/1‑140 (MQ=255)         tGACGCGCTTTTCACCGACGACAAAAAAGCGTTCGGTGATATTTTCACTTTCGACTTCAAGC‑GAGACGGTATTACCCGGCCCCTGGTC‑GCGCGGCAGGTTATGAATATTCTGTTCATGCAGGGAAAAACTCCCCGCCAgt                                                                                                                                >  2:246879/1‑140 (MQ=255)         tGACGCGCTTTTCACCGACGACAAAAAAGCGTTCGGTGATATTTTCACTTTCGACTTCAAGC‑GAGACGGTATTACCCGGCCCCTGGTC‑GCGCGGCAGGTTATGAATATTCTGTTCATGCAGGGAAAAACTCCCCGCCAgt                                                                                                                                >  1:1838479/1‑140 (MQ=255)         tGACGCGCTTTTCACCGACGACAAAAAAGCGTTCGGTGATATTTTCACTTTCGACTTCAAGC‑GAGACGGTATTACCCGGCCCCTGGTC‑GCGCGGCAGGTTATGAATATTCTGTTCATGCAGGGAAAAACTCCCCGCCAgg                                                                                                                                >  1:1918913/1‑139 (MQ=255)           aCGCGCTTTTCACCGACGACAAAAAAGCGTTCGGTGATATTTTCACTTTCGACTTCAAGC‑GAGACGGTATTACCCGGCCCCTGGTC‑GCGCGGCAGGTTATGAATATTCTGTTCATGCAGGGAAAAACTCCCCGCCAgtgt                                                                                                                              >  1:367665/1‑140 (MQ=255)                     acccGACGAAAAAAAAGGTTTGGGTCTTTTTTTCTCTTTC‑ACTTACAGCAAAGACTTAATCACCCGGCCCTGGGTC‑GGGGGGCAGTTTATGAATATTTTGTCGATGCAGAAAAAACCCCCCCGCCAGTGTAGCGATTTCa                                                                                                                    <  2:2422036/138‑1 (MQ=255)                       ccGACGACAAAAAAGCGTGGGGTGATTTTTCCGTTTTGGTCTTCAAGA‑GAGAGGGTTTTCCCCGGCCCCTGGTC‑GCGCGGCAGTTTATGAATTTTCTGTTCAGGAAGGGAAAACCCCCCCGCCAGTGTAGCGATTTCACg                                                                                                                  <  2:314321/140‑1 (MQ=255)                       ccGACAAAAAAAAGGCGTGGGGTGATTTTTTCACCCTCGATTTAAAGG‑GAGACGGTTTCCCCCGCCCCCTGGCC‑GCGCGGCAGTTTATGAATTTTCTTTTAATGAAGGAAAAACCTCCCCGCCAGTGTAGCGATTTCACg                                                                                                                  <  2:92321/140‑1 (MQ=255)                       ccGACAAAAAAAAAGCTTTCGGTGATATTTTCCCTTTCGACTTCAAGG‑GAGACGATATCCCCCGCCCCCTGGTC‑CCGCGGGAGGTTATAAATATTCTTTTCATGCAGGAAAAACCCCCCCGCCAGTGTAGCGATTTCACg                                                                                                                  <  2:442731/140‑1 (MQ=255)                           cgacAAAAAAGCGTTCGGTGATATTTTCACTTTCGACTTCAAGC‑GAGACGGTATTACCCGGCCCCTGGTC‑GCGCGGCAGGTTATGAATATTCTGTTCATGCAGGGAAAAACTCCCCGCCAGTGTAGCGATTTCACGCTCa                                                                                                              >  2:1333589/1‑140 (MQ=255)                             acAAAAAAGCGTTCGGTGATATTTTCACTTTCGACTTCAAGC‑GAGACGGTATTACCCGGCCCCTGGTC‑GCGCGGCAGGTTATGAATATTCTGTTCATGCAGGGAAAAACTCCCCGCCAGTGTAGCGATTTCACGCTCAGc                                                                                                            >  1:515671/1‑140 (MQ=255)                                      ggTTGGGTTATTTTTTCATTTTCGACTAAAAGC‑GAGACGTTATT‑CCCGGCCCCTGGGCGGCGGGGACATTTGTGAATATTAGTTAGATGGAGGGAAAACCCCTCCGCCAGTGTAGCGATTTCACGCTCAGCAACATGgcg                                                                                                   <  2:1915191/139‑1 (MQ=255)                                           ggTGATTTTTCCCCTTTCAACTTAAAGC‑GAGACGTTATCCCCCGCCCCCGGGCC‑CCGCGGCAGTTTATAAATATTCTGTCCATGCAGAAAAAACCCCCCCGCCAGTGTAGCGATTTCACGCTCAGCAACATGGCGCGGca                                                                                              <  2:115553/140‑1 (MQ=255)                                             tGATATTTTCACTTTCGACTTCAAGC‑GAGACGGTATTACCCGGCCCCTGGTC‑GCGCGGCAGGTTATGAATATTCTGTTCATGCAGGGAAAAACTCCCCGCCAGTGTAGCGATTTCACGCTCAGCAACATGGCGCGGcaca                                                                                            >  1:2124443/1‑140 (MQ=255)                                                ttttttCTCTTTCGACTTCAAGC‑GAGACGTTATTCCCCGCCCCCTGGTC‑GGGCGGCAGTTTATAAATATTCTGTTCATGCAGGAAAAACCCCCCCGCCAGTGTAGCGATTTCACGCTCAGCAACATGGCGCGGCACACCa                                                                                         <  2:515671/138‑1 (MQ=255)                                                  ttttCTCTTTCGACTTAAAGA‑GAGACGGTTTTCCCCGCCCCCTGGTC‑GCGCGGCGGTTTATGAATTTTCTTTTCATGGAGGAAAAACCCCCCCGCCAGTGTAGCGATTTCACGCTCAGCAACATGGCGCGGCACACCAGc                                                                                       <  2:1713994/140‑1 (MQ=255)                                                     tctttttCGACTTAAAGC‑GAGAGGTTTTTCCCCGGCCCCTGGTC‑GCGCGGCAGTTTATAAATTTTCTTTTCAGGCAGGAAAAACCCCCCCGCCAGTGTAGCGATTTCACGCTCAGCAACATGGCGCGGCACACCAGCTaa                                                                                    <  2:1030857/136‑1 (MQ=255)                                                     tCACTTTCGACTTCAAGC‑GAGACGGTATTACCCGGCCCCTGGTC‑GCGCGGCAGGTTATGAATATTCTGTTCATGCAGGGAAAAACTCCCCGCCAGTGTAGCGATTTCACGCTCAGCAACATGGCGCGGCACACCAGCTaa                                                                                    >  1:1342257/1‑140 (MQ=255)                                                       aCTTTCGACTTCAAGC‑GAGACGGTATTACCCGGCCCCTGGTC‑GCGCGGCAGGTTATGAATATTCTGTTCATGCAGGGAAAAACTCCCCGCCAGTGTAGCGATTTCACGCTCAGCAACATGGCGCGGCACACCAGCTAATa                                                                                  >  1:516820/1‑140 (MQ=255)                                                         tttCGACTTCAAGC‑GAGACGGTATTACCCGGCCCCTGGTC‑GCGCGGCAGGTTATGAATATTCTGTTCATGCAGGGAAAAACTCCCCGCCAGTGTAGCGATTTCACGCTCAGCAACATGGCGCGGCACACCAGCTAATAGa                                                                                >  1:1740531/1‑140 (MQ=255)                                                         tttCGACTTCAAGC‑GAGACGGTATTACCCGGCCCCTGGTC‑GCGCGGCAGGTTATGAATATTCTGTTCATGCAGGGAAAAACTCCCCGCCAGTGTAGCGATTTCACGCTCAGCAACATGGCGCGGCACACCAGCTAATAGa                                                                                >  1:956708/1‑140 (MQ=255)                                                         tttCGACTTCAAGC‑GAGACGGTATTACCCGGCCCCTGGTC‑GCGCGGCAGGTTATGAATATTCTGTTCATGCAGGGAAAAACTCCCCGCCAGTGTAGCGATTTCACGCTCAGCAACATGGCGCGGCACACCAGCTAATAGa                                                                                >  1:1625088/1‑140 (MQ=255)                                                         tttCGACTTCAAGC‑GAGACGGTATTACCCGGCCCCTGGTC‑GCGCGGCAGGTTATGAATATTCTGTTCATGCAGGGAAAAACTCCCCGCCAGTGTAGCGATTTCACGCTCAGCAACATGGCGCGGCACACCAGCTAATAGa                                                                                >  1:1542155/1‑140 (MQ=255)                                                          ttCGACTTCAAGC‑GAGACGGTATTACCCGGCCCCTGGTC‑GCGCGGCAGGTTATGAATATTCTGTTCATGCAGGGAAAAACTCCCCGCCAGTGTAGCGATTTCACGCTCAGCAACATGGCGCGGCACACCAGCTAATAGaa                                                                               >  1:2252431/1‑140 (MQ=255)                                                            cGACTTCAAGC‑GAGACGGTATTACCCGGCCCCTGGTC‑GCGCGGCAGGTTATGAATATTCTGTTCATGCAGGGAAAAACTCCCCGCCAGTGTAGCGATTTCACGCTCAGCAACATGGCGCGGCACACCAGCTAATAGAACt                                                                             >  1:2396777/1‑140 (MQ=255)                                                                 tCAAGC‑GAGACGGTATTACCCGGCCCCTGGTC‑GCGCGGCAGGTTATGAATATTCTGTTCATGCAGGGAAAAACTCCCCGCCAGTGTAGCGATTTCACGCTCAGCAACATGGCGCGGCACACCAGCTAATAGAACTTCTcc                                                                        >  1:1912240/1‑140 (MQ=255)                                                                  cAAGC‑GAGACGGTATTACCCGGCCCCTGGTC‑GCGCGGCAGGTTATGAATATTCTGTTCATGCAGGGAAAAACTCCCCGCCAGTGTAGCGATTTCACGCTCAGCAACATGGCGCGGCACACCAGCTAATAGAACTTCTCCa                                                                       >  1:2167547/1‑140 (MQ=255)                                                                  aaaGG‑GAGACGGTATCCCCCGCCCCCGGGTC‑GCGGGGCAGTTTATAAATTTTCTTTTCAGGAAGGAAAAACCCCCCCGCCAGTGTAGCGATTTCACGCTCAGCAACATGGCGCGGCACACCAGCTAATAGAACTTCTCCa                                                                       <  2:1838479/139‑1 (MQ=255)                                                                  aaaGC‑GAGAGGGTATTACCCGCCCCTTGGTC‑CGGCGGCGGTTTATAAATTTTCTTTCCAGGCAGGAAAAACCCCCCCGCCAGTGTAGCGATTTCACGCTCAGCAACATGGCGCGGCACACCAGCTAATAGAACTTCTCCa                                                                       <  2:1542155/139‑1 (MQ=255)                                                                  aaaGC‑GAGACGTTATTCCCCGCCCCCTGGCC‑GCGCGGCAGTTTATAAATTTCCTTTTCAGGAAGGAAAAACCCCCCCGCCAGTGTAGCGATTTCACGCTCAGCAACATGGCGCGGCACACCAGCTAATAGAACTTCTCCa                                                                       <  2:1912240/139‑1 (MQ=255)                                                                       ggagaCGTTATTCCCCGCCCCTTGGCC‑GCGCGGCAGTTTATAAATATTCTGTACATGCAGGAAAAACCCCCCCGCCAGTGTAGCGATTTCACGCTCAGCAACATGGCGCGGCACACCAGCTAATAGAACTTCTCCACGCa                                                                   <  2:2396777/139‑1 (MQ=255)                                                                        gagaGGTTATTCCCCGCCCCCGGGTC‑GCGGGGAAGTTTATGAATTTTCTTTTCAGGCAGGAAAAACCCCCCCGCCAGTGTAGCGATTTCACGCTCAGCAACATGGCGCGGCACACCAGCTAATAGAACTTCTCCACGCAt                                                                  <  2:2124443/140‑1 (MQ=255)                                                                        gagaCGGTATCCCCCGGCCCCTGGTC‑GCGGGGCAGTTTATAAATTTTCTGTTCAGGCAGGAAAAACCCCCCCGCCAGTGTAGCGATTTCACGCTCAGCAACATGGCGCGGCACACCAGCTAATAGAACTTCTCCACGCAt                                                                  <  2:2434952/140‑1 (MQ=255)                                                                          gaCGGTATTACCCGGCCCCTGGTC‑GCGCGGCAGGTTATGAATATTCTGTTCATGCAGGGAAAAACTCCCCGCCAGTGTAGCGATTTCACGCTCAGCAACATGGCGCGGCACACCAGCTAATAGAACTTCTCCACGCATCt                                                                >  2:2057372/1‑140 (MQ=255)                                                                           aCGGTATTACCCGGCCCCTGGTC‑GCGCGGCAGGTTATGAATATTCTGTTCATGCAGGGAAAAACTCCCCGCCAGTGTAGCGATTTCACGCTCAGCAACATGGCGCGGCACACCAGCTAATAGAACTTCTCCACGCATCTg                                                               >  1:2434952/1‑140 (MQ=255)                                                                             tttatcccccGCCCCGGGGTC‑GCGCGGCGGGTTATTAATTTTCTGTTCAGACGGGAAAAAACCCCCCGCCAGTGTAGCGATTTCACGCTCAGCAACATGGCGCGGCACACCAGCTAATAGAACTTCTCCACGCATCTGca                                                             <  2:1081212/133‑1 (MQ=255)                                                                                       ggCCCCTGGTC‑GCGCTGCAGGTTATGAATATTCTGTTCATGCAGGGAAAAACTCCCCGCCAGTGTAGCGATTTCACGCTCAGCAACATGGCGCGGCACACCAGCTAATAGAACTTCTCCACGCATCTGCACAATGTTccc                                                   >  2:1999830/1‑140 (MQ=255)                                                                                       ggCCCCTGGTC‑GCGCGGCAGGTTATGAATATTCTGTTCATGCAGGGAAAAACTCCCCGCCAGTGTAGCGATTTCACGCTCAGCAACATGGCGCGGCACACCAGCTAATAGAACTTCTCCACGCATCTGCACAATGTTccc                                                   >  2:2347652/1‑140 (MQ=255)                                                                                       ggCCCCTGGTC‑GCGCGGCAGGTTATGAATATTCTGTTCATGCAGGGAAAAACTCCCCGCCAGTGTAGCGATTTCACGCTCAGCAACATGGCGCGGCACACCAGCTAATAGAACTTCTCCACGCATCTGCACAATGTTccc                                                   >  1:960534/1‑140 (MQ=255)                                                                                       ggCCCCTGGTC‑GCGCGGCAGGTTATGAATATTCTGTTCATGCAGGGAAAAACTCCCCGCCAGTGTAGCGATTTCACGCTCAGCAACATGGCGCGGCACACCAGCTAATAGAACTTCTCCACGCATCTGCACAATGTTccc                                                   >  1:2180911/1‑140 (MQ=255)                                                                                        gCCCCTGGTC‑GCGCGGCAGGTTATGAATATTCTGTTCATGCAGGGAAAAACTCCCCGCCAGTGTAGCGATTTCACGCTCAGCAACATGGCGCGGCACACCAGCTAATAGAACTTCTCCACGCATCTGCACAATGTTCCcg                                                  >  2:2148270/1‑140 (MQ=255)                                                                                         ccccTGGTC‑GCGCGGCAGGTTATGAATATTCTGTTCATGCAGGGAAAAACTCCCCGCCAGTGTAGCGATTTCACGCTCAGCAACATGGCGCGGCACACCAGCTAATAGAACTTCTCCACGCATCTGCACAATGTTCCcgc                                                 >  1:1081212/1‑140 (MQ=255)                                                                                         ccccTGGTC‑GCGCGGCAGGTTATGAATATTCTGTTCATGCAGGGAAAAACTCCCCGCCAGTGTAGCGATTTCACGCTCAGCAACATGGCGCGGCACACCAGCTAATAGAACTTCTCCACGCATCTGCACAATGTTCCcgc                                                 >  2:1830833/1‑140 (MQ=255)                                                                                         ccccTGGTC‑GCGCGGCAGGTTATGAATATTCTGTTCATGCAGGGAAAAACTCCCCGCCAGTGTAGCGATTTCACGCTCAGCAACATGGCGCGGCACACCAGCTAATAGAACTTCTCCACGCATCTGCACAATGTTCCcgc                                                 >  1:348570/1‑140 (MQ=255)                                                                                         ccccTGGTC‑GCGCGGCAGGTTATGAATATTCTGTTCATGCAGGGAAAAACTCCCCGCCAGTGTAGCGATTTCACGCTCAGCAACATGGCGCGGCACACCAGCTAATAGAACTTCTCCACGCATCTGCACAATGTTCCcgc                                                 >  2:1978181/1‑140 (MQ=255)                                                                                         ccccGGGCC‑GCGCGGCAGTTTATAAATTTTCTTTTCATGAAGGAAAAACCCCCCCGCCAGTGTAGCGATTTCACGCTCAGCAACATGGCGCGGCACACCAGCTAATAGAACTTCTCCACGCATCTGCACAATGTTCCcgc                                                 <  2:2167547/140‑1 (MQ=255)                                                                                              ggTC‑GCGCGGCAGGTTATGAATATTCTGTTCATGCAGGGAAAAACTCCCCGCCAGTGTAGCGATTTCACGCTCAGCAACATGGCGCGGCACACCAGCTAATAGAACTTCTCCACGCATCTGCACAATGTTCCCGCGCTCg                                            >  1:1606171/1‑140 (MQ=255)                                                                                              ggTC‑GCGCGGCAGGTTATGAATATTCTGTTCATGCAGGGAAAAACTCCCCGCCAGTGTAGCGATTTCACGCTCAGCAACATGGCGCGGCACACCAGCTAATAGAACTTCTCCACGCATCTGCACAATGTTCCCGCGCTCg                                            >  1:246130/1‑140 (MQ=255)                                                                                                    cgcgGCAGGTTATGAATATTCTGTTCATGCAGGGAAAAACTCCCCGCCAGTGTAGCGATTTCACGCTCAGCAACATGGCGCGGCACACCAGCTAATAGAACTTCTCCACGCATCTGCACAATGTTCCCGCGCTCGCCAAg                                       >  2:168886/1‑140 (MQ=255)                                                                                                    cgcgGCAGGTTATGAATATTCTGTTCATGCAGGGAAAAACTCCCCGCCAGTGTAGCGATTTCACGCTCAGCAACATGGCGCGGCACACCAGCTAATAGAACTTCTCCACGCATCTGCACAATGTTCCCGCGCTCGCCAAg                                       >  1:1108435/1‑140 (MQ=255)                                                                                                    cgcgGCAGGTTATGAATATTCTGTTCATGCAGGGAAAAACTCCCCGCCAGTGTAGCGATTTCACGCTCAGCAACATGGCGCGGCACACCAGCTAATAGAACTTCTCCACGCATCTGCACAATGTTCCCGCGCTCGCCAAg                                       >  1:1750470/1‑140 (MQ=255)                                                                                                    cgcgGCAGGTTATGAATATTCTGTTCATGCAGGGAAAAACTCCCCGCCAGTGTAGCGATTTCACGCTCAGCAACATGGCGCGGCACACCAGCTAATAGAACTTCTCCACGCATCTGCACAATGTTCCCGCGCTCGCCAAg                                       >  1:600750/1‑140 (MQ=255)                                                                                                         gggtttgtGAATATTTTGTTCAAGCGGGAAAAACCCCCCCGCCAGTGTAGCGATTTCACGCTCAGCAACATGGCGCGGCACACCAGCTAATAGAACTTCTCCACGCATCTGCACAATGTTCCCGCGCTCGCCAAGTTGCa                                  <  2:960534/133‑1 (MQ=255)                                                                                                         gagtttATAAATTTTTTGTTCATGCAGGAAAAACCCCCCCGCCAGTGTAGCGATTTCACGCTCAGCAACATGGCGCGGCACACCAGCTAATAGAACTTCTCCACGCATCTGCACAATGTTCCCGCGCTCGCCAAGTTGCa                                  <  2:1342257/136‑1 (MQ=255)                                                                                                         cAGTTTATAAATTTTTTTTTCATGCAGGAAAAACCCCCCCGCCAGTGTAGCGATTTCACGCTCAGCAACATGGCGCGGCACACCAGCTAATAGAACTTCTCCACGCATCTGCACAATGTTCCCGCGCTCGCCAAGTTGCa                                  <  2:1606171/140‑1 (MQ=255)                                                                                                          gggTTATAAATATTCTGTTCAGGAAGGAAAAACCCCCCCGCCAGTGTAGCGATTTCACGCTCAGCAACATGGCGCGGCACACCAGCTAATAGAACTTCTCCACACATCTGCACAATGTTCCCGCGCTCGCCAAGTTGCaa                                 <  1:246879/139‑1 (MQ=255)                                                                                                          acagtATGAATTTTCTGTTCAGGCAGGAAAAACCCCCCCGCCAGTGTAGCGATTTCACGCTCAGCAACATGGCGCGGCACACCAGCTAATAGAACTTCTCCACGCATCTGCACAATGTTCCCGCGCTCGCCAAGTTGCaa                                 <  2:645032/136‑1 (MQ=255)                                                                                                                     atTCTGTTCATGCAGGGAAAAACTCCCCGCCAGTGTAGCGATTTCACGCTCAGCAACATGGCGCGGCACACCAGCTAATAGAACTTCTCCACGCATCTGCACAATGTTCCCGCGCTCGCCAAGTTGCAAGGTGTTAAACg                      >  1:608863/1‑140 (MQ=255)                                                                                                                      ttCTGTTCATGCAGGGAAAAACTCCCCGCCAGTGTAGCGATTTCACGCTCAGCAACATGGCGCGGCACACCAGCTAATAGAACTTCTCCACGCATCTGCACAATGTTCCCGCGCTCGCCAAGTTGCAAGGTGTTAAACGa                     >  2:1326714/1‑140 (MQ=255)                                                                                                                      ttCTGTTCATGCAGGGAAAAACTCCCCGCCAGTGTAGCGATTTCACGCTCAGCAACATGGCGCGGCACACCAGCTAATAGAACTTCTCCACGCATCTGCACAATGTTCCCGCGCTCGCCAAGTTGCAAGGTGTTAAACGa                     >  2:744417/1‑140 (MQ=255)                                                                                                                      ttCTGTTCATGCAGGGAAAAACTCCCCGCCAGTGTAGCGATTTCACGCTCAGCAACATGGCGCGGCACACCAGCTAATAGAACTTCTCCACGCATCTGCACAATGTTCCCGCGCTCGCCAAGTTGCAAGGTGTTAAACGa                     >  2:2246905/1‑140 (MQ=255)                                                                                                                      ttCTGTTCATGCAGGGAAAAACTCCCCGCCAGTGTAGCGATTTCACGCTCAGCAACATGGCGCGGCACACCAGCTAATAGAACTTCTCCACGCATCTGCACAATGTTCCCGCGCTCGCCAAGTTGCAAGGTGTTAAACGa                     >  1:761688/1‑140 (MQ=255)                                                                                                                       tCTGTTCATGCAGGGAAAAACTCCCCGCCAGTGTAGCGATTTCACGCTCAGCAACATGGCGCGGCACACCAGCTAATAGAACTTCTCCACGCATCTGCACAATGTTCCCGCGCTCGCCAAGTTGCAAGGTGTTAAACGAt                    >  2:1364919/1‑140 (MQ=255)                                                                                                                       tCTGTTCATGAAGGGAAAAACTCCCCGCCAGTGTAGCGATTTCACGCTCAGCAACATGGCGCGGCACACCAGCTAATAGAACTTCTCCACGCATCTGCACAATGTTCCCGCGCTCGCCAAGTTGCAAGGTGTTAAACGAt                    >  1:1173068/1‑140 (MQ=255)                                                                                                                         tGTTCATGCAGGGAAAAACTCCCCGCCAGTGTAGCGATTTCACGCTCAGCAACATGGCGCGGCACACCAGCTAATAGAACTTCTCCACGCATCTGCACAATGTTCCCGCGCTCGCCAAGTTGCAAGGTGTTAAACGCTgt                  >  1:645032/1‑139 (MQ=255)                                                                                                                          tttCAGGCAGGAAAAACCCCCCCGCCAGTGTAGCGATTTCACGCTCAGCAACATGGCGCGGCACACCAGCTAATAGAACTTCTCCACGCATCTGCACAATGTTCCCGCGCTCGCCAAGTTGCAAGGTGTTAAACGATGcc                 <  2:1108435/139‑1 (MQ=255)                                                                                                                          gTTCATGCAGGGAAAAACTCCCCGCCAGTGTAGCGATTTCACGCTCAGCAACATGGCGCGGCACACCAGCTAATAGAACTTCTCCACGCATCTGCACAATGTTCCCGCGCTCGCCAAGTTGCAAGGTGTTAAACGATGcc                 >  1:371090/1‑140 (MQ=255)                                                                                                                          gTTCATGCAGGGAAAAACTCCCCGCCAGTGTAGCGATTTCACGCTCAGCAACATGGCGCGGCACACCAGCTAATAGAACTTCTCCACGCATCTGCACAATGTTCCCGCGCTCGCCAAGTTGCAAGGTGTTAAACGATGcc                 >  1:977806/1‑140 (MQ=255)                                                                                                                          gTTCATGCAGGGAAAAACTCCCCGCCAGTGTAGCGATTTCACGCTCAGCAACATGGCGCGGCACACCAGCTAATAGAACTTCTCCACGCATCTGCACAATGTTCCCGCGCTCGCCAAGTTGCAAGGTGTTAAACGATGcc                 >  2:1401615/1‑140 (MQ=255)                                                                                                                           ttCATGCAGGGAAAAACTCCCCGCCAGTGTAGCGATTTCACGCTCAGCAACATGGCGCGGCACACCAGCTAATAGAACTTCTCCACGCATCTGCACAATGTTCCCGCGCTCGCCAAGTTGCAAGGTGTTAAACGATGCCa                >  1:1827480/1‑140 (MQ=255)                                                                                                                           ttCATGCAGGGAAAAACTCCCCGCCAGTGTAGCGATTTCACGCTCAGCAACATGGCGCGGCACACCAGCTAATAGAACTTCTCCACGCATCTGCACAATGTTCCCGCGCTCGCCAAGTTGCAAGGTGTTAAACGATGCCa                >  1:1340731/1‑140 (MQ=255)                                                                                                                           ttCATGCAGGGAAAAACTCCCCGCCAGTGTAGCGATTTCACGCTCAGCAACATGGCGCGGCACACCAGCTAATAGAACTTCTCCACGCATCTGCACAATGTTCCCGCGCTCGCCAAGTTGCAAGGTGTTAAACGATGCCa                >  1:2119225/1‑140 (MQ=255)                                                                                                                           ttCATGCAGGAAAAACCCCCCCGCCAGTGTAGCGATTTCACGCTCAGCAACATGGCGCGGCACACCAGCTAATAGAACTTCTCCACGCATCTGCACAATGTTCCCGCGCTCGCCAAGTTGCAAGGTGTTAAACGATGCCa                <  1:225645/140‑1 (MQ=255)                                                                                                                           ttCATGCAGGAAAAACCCCCCCGCCAGTGTAGCGATTTCACGCTCAGCAACATGGCGCGGCACACCAGCTAATAGAACTTCTCCACGCATCTGCACAATGTTCCCGCGCTCGCCAAGTTGCAAGGTGTTAAACGATGCCa                <  1:1333589/140‑1 (MQ=255)                                                                                                                            tCATGCAGGGAAAAACTCCCCGCCAGTGTAGCGATTTCACGCTCAGCAACATGGCGCGGCACACCAGCTAATAGAACTTCTCCACGCATCTGCACAATGTTCCCGCGCTCGCCAAGTTGCAAGGTGTTAAACGATGCCAc               >  2:328960/1‑140 (MQ=255)                                                                                                                             cATGCAGGGAAAAACTCCCCGCCAGTGTAGCGATTTCACGCTCAGCAACATGGCGCGGCACACCAGCTAATAGAACTTCTCCACGCATCTGCACAATGTTCCCGCGCTCGCCAAGTTGCAAGGTGTTAAACGATGCCACg              >  1:906501/1‑140 (MQ=255)                                                                                                                              aTGCAGGGAAAAACTCCCCGCCAGTGTAGCGATTTCACGCTCAGCAACATGGCGCGGCACACCAGCTAATAGAACTTCTCCACGCATCTGCACAATGTTCCCGCGCTCGCCAAGTTGCAAGGTGTTAAACGATGCCACgg             >  1:2263478/1‑140 (MQ=255)                                                                                                                               tGCAGGGAAAACCCCCCCGCCAGTGTAGCGATTTCACGCTCAGCAACATGGCGCGGCACACCAGCTAATAGAACTTCTCCACGCATCTGCACAATGTTCCCGCGCTCGCCAAGTTGCAAGGTGTTAAACGATGCCACggg            <  1:1401615/140‑1 (MQ=255)                                                                                                                               ggaaGGAAAAACCCCCCCGCCAGTGTAGCGATTTCACGCTCAGCAACATGGCGCGGCACACCAGCTAATAGAACTTCTCCACGCATCTGCACAATGTTCCCGCGCTCGCCAAGTTGCAAGGTGTTAAACGATGCCACggg            <  2:516820/137‑1 (MQ=255)                                                                                                                                gCAGGGAAAAACTCCCCGCCAGTGTAGCGATTTCACGCTCAGCAACATGGCGCGGCACACCAGCTAATAGAACTTCTCCACGCATCTGCACAATGTTCCCGCGCTCGCCAAGTTGCAAGGTGTTAAACGATGCCACGGGc           >  1:1595570/1‑140 (MQ=255)                                                                                                                                gCAGGGAAAAACTCCCCGCCAGTGTAGCGATTTCACGCTCAGCAACATGGCGCGGCACACCAGCTAATAGAACTTCTCCACGCATCTGCACAATGTTCCCGCGCTCGCCAAGTTGCAAGGTGTTAAACGATGCCACGGGc           >  1:1253228/1‑140 (MQ=255)                                                                                                                                gCAGGAAAAACCCCCCCGCCAGTGTAGCGATTTCACGCTCAGCAACATGGCGCGGCACACCAGCTAATAGAACTTCTCCACGCATCTGCACAATGTTCCCGCGCTCGCCAAGTTGCAAGGTGTTAAACGATGCCACGGGc           <  1:2090372/140‑1 (MQ=255)                                                                                                                                    ggAAAAACCCCCCGCCAGTGTAGCGATTTCACGCTCAGCAACATGGCGCGGCACACCAGCTAATAGAACTTCTCCACGCATCTGCACAATGTTCCCGCGCTCGCCAAGTTGCAAGGTGTTAAACGATGCCACGGGCgaga       <  1:1364919/140‑1 (MQ=255)                                                                                                                                     gAAAAACTCCCCGCCAGTGTAGCGATTTCACGCTCAGCAACATGGCGCGGCACACCAGCTAATAGAACTTCTCCACGCATCTGCACAATGTTCCCGCGCTCGCCAAGTTGCAAGGTGTTAAACGATGCCACGGGCGAGAc      >  2:2144934/1‑140 (MQ=255)                                                                                                                                      aaaaaCTCCCCGCCAGTGTAGCGATTTCACGCTCAGCAACATGGCGCGGCACACCAGCTAATAGAACTTCTCCACGCATCTGCACAATGTTCCCGCGCTCGCCAAGTTGCAAGGTGTTAAACGATGCCACGGGCGAGACt     <  1:2347652/140‑1 (MQ=255)                                                                                                                                        aaaCTCCCCGCCAGTGTAGCGATTTCACGCTCAGCATCATGGCGCGGAACACCAGCTAATAGAACTTCTCCACGCATCTGCACAATGTTCCCGCGATCGCCAAGTTGCAAGGTGATAAACGATTCCACGGGCGAGACTTc   >  2:1264610/1‑140 (MQ=255)                                                                                                                                         aaCTCCCCGCCAGTGTAGCGATTTCACGCTCAGCAACATGGCGCGGCACACCAGCTAATAGAACTTCTCCACGCATCTGCACAATGTTCCCGCGCTCGCCAAGTTGCAAGGTGTTAAACGATGCCACGGGCGAGACTTcc  <  2:977806/140‑1 (MQ=255)                                                                                                                                          |                                                                                                                                           CTCGGCACTGACGCGCTTTTCACCGACGACAAAAAAGCGTTCGGTGATATTTTCACTTTCGACTTCAAGC‑GAGACGGTATTACCCGGCCCCTGGTC‑GCGCGGCAGGTTATGAATATTCTGTTCATGCAGGGAAAAACTCCCCGCCAGTGTAGCGATTTCACGCTCAGCAACATGGCGCGGCACACCAGCTAATAGAACTTCTCCACGCATCTGCACAATGTTCCCGCGCTCGCCAAGTTGCAAGGTGTTAAACGATGCCACGGGCGAGACTTCC  >  NC_000913/3556070‑3556343

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑