Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	2,667,126	0	A	C	25.4%	124.8 / 13.2	67	Y241D (TAT→GAT)	hcaT	putative 3‑phenylpropionate transporter
Reads supporting (aligned to +/- strand):  ref base A (34/16);  new base C (0/17);  total (34/33)
Fisher's exact test for biased strand distribution p-value = 3.46e-07
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00
Rejected as consensus: Frequency below/above cutoff threshold.

GCTCCCATAATGCCCCAGCGCACTACGCCGCAAATCGCCGAGATCAACAGCATATCGCGTGCACTACAACGGCGGAAAAGTTTATTACTCAGCGCAAAGATAATGACTTCCGCCACCACGCCCAGCGACCACAAATACCCCACCGCCGAGGCCGAGTAGCCAGCTGCCTGCCAGTAAATGGCGCTAAAACCGTAATAGGCCGCATGTGCCCCCTGCAATAAACAAACGCAGGCCAGAAAGCGCCAGTTCTGGCGAACCAGCGCCAACCACGCAGAC  >  NC_000913/2666990‑2667265                                                                                                                                         |                                                                                                                                            gCTCCCATAATGCCCCAGCGCACTACGCCGCAAATCGCCGAGATCAACAGCATATCGCGTGCACTACAACGGCGGAAAAGTTTATTACTCAGCGCAAAGATAATGACTTCCGCCACCACGCCCAGCGACCACAAATAccc                                                                                                                                          >  2:622271/1‑140 (MQ=255)    cccATAATGCCCCAGCGCACTACGCCGCAAATCGCCGAGATCAACAGCATATCGCGTGCACTACAACGGCGGAAAAGTTTATTACTCAGCGCAAAGATAATGACTTCCGCCACCACGCCCAGCGACCACAAATACCCCAc                                                                                                                                       >  2:1247990/1‑140 (MQ=255)    cccATAATGCCCCAGCGCACTACGCCGCAAATCGCCGAGATCAACAGCATATCGCGTGCACTACAACGGCGGAAAAGTTTATTACTCAGCGCAAAGATAATGACTTCCGCCACCACGCCCAGCGACCACAAATACCCCAc                                                                                                                                       >  1:1139609/1‑140 (MQ=255)       aTAATGCCCCAGCGCACTACGCCGCAAATCGCCGAGATCAACAGCATATCGCGTGCACTACAACGGCGGAAAAGTTTATTACTCAGCGCAAAGATAATGACTTCCGCCACCACGCCCAGCGACCACAAATACCCCAccgc                                                                                                                                    <  1:1342655/140‑1 (MQ=255)           tGCCCCAGCGCACTACGCCGCAAATCGCCGAGATCAACAGCATATCGCGTGCACTACAACGGCGGAAAAGTTTATTACTCAGCGCAAAGATAATGACTTCCGCCACCACGCCCAGCGACCACAAATCCCCCACCgccgag                                                                                                                                <  2:502695/140‑1 (MQ=255)           tGCCCCAGCGCACTACGCCGCAAATCGCCGAGATCAACAGCATATCGCGTGCACTACAACGGCGGAAAAGTTTATTACTCAGCGCAAAGATAATGACTTCCGCCACCACGCCCAGCGACCACAAATACCCCACCgccgag                                                                                                                                <  2:1148666/140‑1 (MQ=255)               ccAGCGCACTACGCCGCAAATCGCCGAGATCAACAGCATATCGCGTGCACTACAACGGCGGAAAAGTTTATTACTCAGCGCAAAGATAATGACTTCCGCCACCACGCCCAGCGACCACAAATACCCCACCgccgaggccg                                                                                                                            >  2:1384009/1‑140 (MQ=255)                 aGCGCACTACGCCGCAAATCGCCGAGATCAACAGCATATCGCGTGCACTACAACGGCGGAAAAGTTTATTACTCAGCGCAAAGATAATGACTTCCGCCACCACGCCCAGCGACCACAAATACCCCACCgccgaggccgag                                                                                                                          >  2:931880/1‑140 (MQ=255)                 aGCGCACTACGCCGCAAATCGCCGAGATCAACAGCATATCGCGTGCACTACAACGGCGGAAAAGTTTATTACTCAGCGCAAAGATAATGACTTCCGCCACCACGCCCAGCGACCACAAATACCCCACCgccgaggccgag                                                                                                                          >  1:425742/1‑140 (MQ=255)                 aGCGCACTACGCCGCAAATCGCCGAGATCAACAGCATATCGCGTGCACTACAACGGCGGAAAAGTTTATTACTCAGCGCAAAGATAATGACTTCCGCCACCACGCCCAGCGACCACAAATACCCCACCgccgaggccgag                                                                                                                          >  1:220171/1‑140 (MQ=255)                 aGCGCACTACGCCGCAAATCGCCGAGATCAACAGCATATCGCGTGCACTACAACGGCGGAAAAG‑TTATTACTCAGCGCAAAGATAATGACTTCCGCCACCACGCCCAGCGACCACAAATACCCCACCGCCGAGGCCGAGt                                                                                                                         >  2:790848/1‑140 (MQ=255)                  gcgcACTACGCCGCAAATCGCCGAGATCAACAGCATATCGCGTGCACTACAACGGCGGAAAAGTTTATTACTCAGCGCAAAGATAATGACTTCCGCCACCACGCCCAGCGACCACAAATACCCCACCGCCGAGGCCGAGt                                                                                                                         >  1:422477/1‑140 (MQ=255)                        tACGCCGCAAATCGCCGAGATCAACAGCATATCGCGTGCACTACAACGGCGGAAAAGTTTATTACTCAGCGCAAAGATAATGACTTCCGCCACCACGCCCAGCGACCACAAATACCCCACCGCCGAGGCCGAGTAGCCAg                                                                                                                   <  1:622271/140‑1 (MQ=255)                         aCGCCGCAAATCGCCGAGATCAACAGCATATCGCGTGCACTACAACGGCGGAAAAGTTTATTACTCAGCGCAAAGATAATGACTTCCGCCACCACGCCCAGCGACCACAAATACCCCACCGCCGAGGCCGAGTAGCCAGc                                                                                                                  >  2:107915/1‑140 (MQ=255)                            ccgcAAATCGCCGAGATCAACAGCATATCGCGTGCACTACAACGGCGGAAAAGTTTATTACTCAGCGCAAAGATAATGACTTCCGCCACCACGCCCAGCGACCACAAATACCCCACCGCCGAGGCCGAGTAGCCAGctgc                                                                                                               >  1:962393/1‑140 (MQ=255)                             cgcAAATCGCCGAGATCAACAGCATATCGCGTGCACTACAACGGCGGAAAAGTTTATTACTCAGCGCAAAGATAATGACTTCCGCCACCACGCCCAGCGACCACAAATCCCCCACCGCCGAGGCCGAGTAGCCAGctgcc                                                                                                              <  2:413809/140‑1 (MQ=255)                             cgcAAATCGCCGAGATCAACAGCATATCGCGTGCACTACAACGGCGGAAAAGTTTATTACTCAGCGCAAAGATAATGACTTCCGCCACCACGCCCAGCGACCACAAATACCCCACCGCCGAGGCCGAGTAGCCAGctgcc                                                                                                              >  1:824658/1‑140 (MQ=255)                             cgcAAATCGCCGAGATCAACAGCATATCGCGTGCACTACAACGGCGGAAAAGTTTATTACTCAGCGCAAAGATAATGACTTCCGCCACCACGCCCAGCGACCACAAATACCCCACCGCCGAGGCCGAGTAGCCAGctgcc                                                                                                              >  2:391560/1‑140 (MQ=255)                                aaaTCGCCGAGATCAACAGCATATCGCGTGCACTACAACGGCGGAAAAGTTTATTACTCAGCGCAAAGATAATGACTTCCGCCACCACGCCCAGCGACCACAAATACCCCACCGCCGAGGCCGAGTAGCCAGctgcctgc                                                                                                           <  1:391560/140‑1 (MQ=255)                                              aaCAGCATATCGCGTGCACTACAACGGCGGAAAAGTTTATTACTCAGCGCAAAGATAATGACTTCCGCCACCCCGCCCAGCGTCCACAAATCCCCCCCCGCCGAGGCCGAGTAGCCAGCTGCCTGCCAGTAAATGGCGCt                                                                                             <  2:525598/140‑1 (MQ=255)                                              aaCAGCATATCGCGTGCACTACAACGGCGGAAAAGTTTATTACTCAGCGCAAAGATAATGACTTCCGCCACCACGCCCAGCGACCACAAATCCCCCACCGCCGAGGCCGAGTAGCCAGCTGCCTGCCAGTAAATGGCGCt                                                                                             <  1:964755/140‑1 (MQ=255)                                                cAGCATCTCGCGTGCACTACAACGGCGGAAAAGTTTATTACTCAGCGCAAAGATAATGCCTTCCCCCACCACGCCCAGCGACCACAAATCCCCCACCGCCGAGGCCGAGTAGCCAGCTGCCTGCCAGTAAATGGCGCTaa                                                                                           <  2:886101/140‑1 (MQ=255)                                                  gCATATCGCGTGCCCTACAAGGGCGGAAAGTTTTATCTCGCGGCGCAAAGATAATGGCTGCCCCCACCGCGCCTACCAACCAAAAATCCCCCGCCGCCGAGGCCGAGTAGCCAGCTGCCTGCCAGTAAATGGCGCTaaaa                                                                                         <  2:422477/140‑1 (MQ=255)                                                  gCATATCGCGTGCACTACAACGGCGGAAAAGTTTATTACTCAGCGCAAAGATAATGACTTCCGCCCCCACGCCCAGCGACCACAAATACCCCACCGCCGAGGCCGAGTAGCCAGCTGCCTGCCAGTAAATGGCGCTaaaa                                                                                         <  1:1247990/140‑1 (MQ=255)                                                  gCATATCGCGTGCACTACAACGGCGGAAAAGTTTATTACTCAGCGCAAAGATAATGACTTCCGCCACCACGCCCAGCGACCACAAATACCCCACCGCCGAGGCCGAGTAGCCAGCTGCCTGCCAGTAAATGGCGCTaaaa                                                                                         <  1:107915/140‑1 (MQ=255)                                                  gCATATCGCGTGCACTACAACGGCGGAAAAGTTTATTACTCAGCGCAAAGATAATGACTTCCGCCACCACGCCCAGCGACCAAAAATACCCCACCGCCGAGGCCGAGTAGCCAGCTGCCTGCCAGTAAATGGCGCTaaaa                                                                                         <  1:487034/140‑1 (MQ=255)                                                        cgcgTGCACTACAACGGCGGAAAAGTTTATTACTCAGCGCAAAGATAATGACTTCCGCCACCACGCCCAGCGACCACAAATCCCCCACCGCCGAGGCCGAGTAGCCAGCTGCCTGCCAGTAAATGGCGCTAAAACCGTaa                                                                                   <  2:1010842/140‑1 (MQ=255)                                                        cgcgTGCACTACAACGGCGGAAAAGTTTATTACTCAGCGCAAAGATAATGACTTCCGCCACCACGCCCAGCGACCACAAATACCCCACCGCCGAGGCCGAGTAGCCAGCTGCCTGCCAGTAAATGGCGCt                                                                                             >  2:276448/1‑130 (MQ=255)                                                        cgcgTGCACTACAACGGCGGAAAAGTTTATTACTCAGCGCAAAGATAATGACTTCCGCCACCACGCCCAGCGACCACAAATACCCCACCGCCGAGGCCGAGTAGCCAGCTGCCTGCCAGTAAATGGCGCt                                                                                             <  1:276448/130‑1 (MQ=255)                                                        cgcgTGCACTACAACGGCGGAAAAGTTTATTACTCAGCGCAAAGATAATGACTTCCGCCACCACGCCCAGCGACCACAAATACCCCACCGCCGAGGCCGAGTAGCCAGCTGCCTGCCAGTAAATGGCGCTAAAACCGTaa                                                                                   >  1:1010842/1‑140 (MQ=255)                                                          cgTGCACTACAACGGCGGAAAAGTTTATTACTCAGCGCAAAGATAATGACTTCCGCCACCACGCCCAGCGACCACAAATACCCCACCGCCGAGGCCGAGTAGCCAGCTGCCTGCCAGTAAATGGCGCTAAAACCGTAATa                                                                                 >  1:875344/1‑140 (MQ=255)                                                           gTGCACTACAACGGCGGAAAAG‑TTATTACTCAGCGCAAAGATAATGACTTCCGCCACCACGCCCAGCGACCACAAATACCCCACCGCCGAGGCCGAGTAGCCAGCTGCCTGCCAGTAAATGGCGCTAAAACCGTAATAgg                                                                               <  1:790848/140‑1 (MQ=255)                                                             gCACTCCACCGGCGGAAAAGTTTATTCCTCAGCGCAAAGATAATGACTGCCGCCACCACGCCCAGCGCCCACAAATACCCCACCGCCGAGGCCGAGTAGCCAGCTGCCTGCCAGTAAATGGCGCTAAAACCGTAATAGGc                                                                              <  2:661832/140‑1 (MQ=255)                                                             gCACTACAACGGCGGAAAAGTTTATTACTCAGCGCAAAGATAATGACTTCCGCCACCACGCCCAGCGACCACAAATACCCCACCGCCGAGGCCGAGTAGCCAGCTGCCTGCCAGTAAATGGCGCTAAAACCGTAATAGGc                                                                              <  2:962393/140‑1 (MQ=255)                                                             gCACTACAACGGCGGAAAAGTTTATTACTCAGCGCAAAGATAATGACTTCCGCCACCACGCCCAGCGACCACAAATACCCCACCGCCGAGGCCGAGTAGCCAGCTGCCTGCCAGTAAATGGCGCTAAAACCGTAATAGGc                                                                              <  2:824658/140‑1 (MQ=255)                                                               aCTACAACGGCGGAAAAGTTTATTACTCAGCGCAAAGATAATGACTTCCGCCACCGCGCCCAGCGACCACAAATACCCCACCGCCGAGGCCGAGTAGCCAGCTGCCTGCCAGTAAATGGCGCTAAAACCGTAATAGGCCg                                                                            <  1:7725/140‑1 (MQ=255)                                                               aCTACAACGGCGGAAAAGTTTATTACTCAGCGCAAAGATAATGACTTCCGCCACCACGCCCAGCGACCACAAATCCCCCACCGCCGAGGCCGAGTAGCCAGCTGCCTGCCAGTAAATGGCGCTAAAACCGTAATAGGCCg                                                                            <  2:1142634/140‑1 (MQ=255)                                                                      cggcggAAAAGTTTATTACTCAGCGCAAAGATAATGACTTCCGCCACCACGCCCAGCGACCACAAATACCCCACCGCCGAGGCCGAGTAGCCAGCTGCCTGCCAGTAAATGGCGCTAAAACCGTAATAGGCCGCATGTGc                                                                     >  1:1319819/1‑140 (MQ=255)                                                                       ggcggAAAAGTTTATTACTCAGCGCAAAGATAATGACTTCCGCCACCACGCCCAGCGACCACAAATACCCCACCGCCGAGGCCGAGTAGCCAGCTGCCTGCCAGTAAATGGCGCTAAAACCGTAATAGGCCGCATGTGcc                                                                    <  2:1139609/140‑1 (MQ=255)                                                                         cggAAAAGTTTATTCCTCAGCGCAAAGATAATGACTCCCGCCACCACGCCCAGCGACCACAAATCCCCCACCGCCGAGGCCGAGTAGCCAGCTGCCTGCCAGTAAATGGCGCTAAAACCGTAATAGGCCGCATGTGcccc                                                                  <  2:834834/140‑1 (MQ=255)                                                                           gAAAAGTTTATTACTCAGCGCAAAGATAATGACTTCCGCCACCACGCCCAGCGACCACAAATACCCCACCGCCGAGGCCGAGTAGCCAGCTGCCTGCCAGTAAATGGCGCTAAAACCGTAATAGGCCGCATGTGCCCCCt                                                                >  2:973678/1‑140 (MQ=255)                                                                              aaGTTTATTACTCATCGCAAAGATAATGACTTCCGCCACCACGCCCAGCGACCACAAATCCCCCACCGCCGAGGCCGAGTAGCCAGCTGCCTGCCAGTAAATGGCGCTAAAACCGTAATAGGCCGCATGTGCCCCCTGCa                                                             <  1:342796/140‑1 (MQ=255)                                                                               aGTTTATTACTCAGCGCAAAGATAATGACTTCCGCCACCACGCCCAGCGACCACAAATCCCCCACCGCCGAGGCCGAGTAGCCAGCTGCCTGCCAGTAAATGGCGCTAAAACCGTAATAGGCCGCATGTGCCCCCTGCaa                                                            <  1:908071/140‑1 (MQ=255)                                                                                    tttccccAGCGCAAAGATAATGAGTTCCCCCACCACGCCCAGCGACCACAAATCCCCCACCGCCGAGGCCGAGTAGCCAGCTGCCTGCCAGTAAATGGCGCTAAAACCGTAATAGGCCGCATGTGCCCCCTGCAATaaac                                                       <  2:1319819/134‑1 (MQ=255)                                                                                        cTCAGCGCAAAGATAATGACTTCCGCCACCACGCCCAGCGACCACGAATACCCCACCGCCGAGGCCGAGTAGCCAGCTGCCTGCCAGTAAATGGCGCTAAAACCGTAATAGGCCGCATGTGCCCCCTGCAATaaacaaac                                                   >  1:981281/1‑140 (MQ=255)                                                                                         tCAGCGCAAAGATAATGACTTCCGCCCCCACGCCCAGCGACCACAAATCCCCCACCGCCGAGGCCGAGTAGCCAGCTGCCTGCCAGTAAATGGCGCTAAAACCGTAATAGGCCGCATGTGCCCCCTGCAATAAACAAACg                                                  <  1:1317859/140‑1 (MQ=255)                                                                                         tCAGCGCAAAGATAATGACTTCCGCCACCACGCCCAGCGACCACAAATCCCCCACCGCCGAGGCCGAGTAGCCAGCTGCCTGCCAGTAAATGGCGCTAAAACCGTAATAGGCCGCATGTGCCCCCTGCAATAAACAAACg                                                  <  1:367025/140‑1 (MQ=255)                                                                                         ccAGCCCAAAGATAATGCCTTCCGCCACCACGCCCAGCGACCACAAATCCCCCACCGCCGAGGCCGAGTAGCCAGCTGCCTGCCAGTAAATGGCGCTAAAACCGTAATAGGCCGCATGTGCCCCCTGCAATAAACAAACg                                                  <  2:220171/139‑1 (MQ=255)                                                                                           aGCGCAAAGATAATGACTTCCGCCACCACGCCCAGCGACCACAAATACCCCACCGCCGAGGCCGAGTAGCCAGCTGCCTGCCAGTAAATGGCGCTAAAACCGTAATAGGCCGCATGTGcccc                                                                  <  1:70374/122‑1 (MQ=255)                                                                                           aGCGCAAAGATAATGACTTCCGCCACCACGCCCAGCGACCACAAATACCCCACCGCCGAGGCCGAGTAGCCAGCTGCCTGCCAGTAAATGGCGCTAAAACCGTAATAGGCCGCATGTGcccc                                                                  >  2:70374/1‑122 (MQ=255)                                                                                           aGCGCAAAGATAATGACTTCCGCCACCACGCCCAGCGACCACAAATACCCCACCGCCGAGGCCGAGTAGCCAGCTGCCTGCCAGTAAATGGCGCTAAAACCGTAATAGGCCGCATGTGCCCCCTGCAATAAACAAACGCa                                                >  2:711363/1‑140 (MQ=255)                                                                                           aGCGCAAAGATAATGACTTCCGCCACCACGCCCAGCGACCACAAATACCCCACCGCCGAGGCCGAGTAGCCAGCTGCCTGCCAGTAAATGGCGCTAAAACCGTAATAGGCCGCATGTGCCCCCTGCAATAAACAAACGCa                                                >  2:564181/1‑140 (MQ=255)                                                                                             cgcAAAGATAATGACTTCCGCCACCACGCCCAGCGACCACAAATACCCCACCGCCGAGGCCGAGTAGCCAGCTGCCTGCCAGTAAATGGCGCTAAAACCGTAATAGGCCGCATGTGCCCCCTGCaa                                                            >  2:763896/1‑126 (MQ=255)                                                                                             cgcAAAGATAATGACTTCCGCCACCACGCCCAGCGACCACAAATACCCCACCGCCGAGGCCGAGTAGCCAGCTGCCTGCCAGTAAATGGCGCTAAAACCGTAATAGGCCGCATGTGCCCCCTGCaa                                                            <  1:763896/126‑1 (MQ=255)                                                                                             cgcAAAGATAATGACTTCCGCCACCACGCCCAGCGACCACAAATACCCCACCGCCGAGGCCGAGTAGCCAGCTGCCTGCCAGTAAATGGCGCTAAAACCGTAATAGGCCGCATGTGCCCCCTGCAATAAACAAACGCAgg                                              >  1:1029073/1‑140 (MQ=255)                                                                                                            ttCCGCCACCACGCCCAGCGACCACAAATACCCCACCGCCGAGGCCGAGTAGCCAGCTGCCTGCCAGTAAATGGCGCTAAAACCGTAATAGGCCGCATGTGCCCCCTGCAATAAACAAACGCAGGCCAGAAAGCGCCAGt                               >  1:1010726/1‑140 (MQ=255)                                                                                                              ccGCCACCACGCCCAGCGACCACAAATACCCCACCGCCGAGGCCGAGTAGCCAGCTGCCTGCCAGTAAATGGCGCTAAAACCGTAATAGGCCGCATGTGCCCCCTGCAATAAACAAACGCAGGCCAGAAAGCGCCAGTTc                             >  1:17017/1‑140 (MQ=255)                                                                                                              ccGCCACCACGCCCAGCGACCACAAATACCCCACCGCCGAGGCCGAGTAGCCAGCTGCCTGCCAGTAAATGGCGCTAAAACCGTAATAGGCCGCATGTGCCCCCTGCAATAAACAAACGCAGGCCAGAAAGCGCCAGTTc                             >  2:642276/1‑140 (MQ=255)                                                                                                              ccGCCACCACGCCCAGCGACCACAAATACCCCACCGCCGAGGCCGAGTAGCCAGCTGCCTGCCAGTAAATGGCGCTAAAACCGTAATAGGCCGCATGTGCCCCCTGCAATAAACAAACGCAGGCCAGAAAGCGCCAGTTc                             >  1:677774/1‑140 (MQ=255)                                                                                                              ccGCCACCACGCCCAGCGACCACAAATACCCCACCGCCGAGGCCGAGTAGCCAGCTGCCTGCCAGTAAATGGCGCTAAAACCGTAATAGGCCGCATGTGCCCCCTGCAATAAACAAACGCAGGCCAGAAAGCGCCAGTTc                             >  2:713742/1‑140 (MQ=255)                                                                                                                     cacGCCCAGCGACCACAAATCCCCCACCGCCGAGGCCGAGTAGCCAGCTGCCTGCCAGTAAATGGCGCTAAAACCGTAATAGGCCGCATGTGCCCCCTGCAATAAACAAACGCAGGCCAGAAAGCGCCAGTTCTGGCGaa                      <  1:1021646/140‑1 (MQ=255)                                                                                                                      acGCCCAGCGACCACAAATACCCCACCGCCGAGGCCGAGTAGCCAGCTGCCTGCCAGTAAATGGCGCTAAAACCGTAATAGGCCGCATGTGCCCCCTGCAATAAACAAACGCAGGCCAGAAAGCGCCAGTTCTGGCGAAc                     >  1:666617/1‑140 (MQ=255)                                                                                                                          ccggCGACCACAAATCCCCCACCGCCGAGGCCGAGTAGCCAGCTGCCTGCCAGTAAATGGCGCTAAAACCGTAATAGGCCGCATGTGCCCCCTGCAATAAACAAACGCAGGCCAGAAAGCGCCAGTTCTGGCGAACCAgc                 <  1:816979/137‑1 (MQ=255)                                                                                                                          ccAGCGACCACAAATACCCCACCGCCGAGGCCGAGTAGCCAGCTGCCTGCCAGTAAATGGCGCTAAAACCGTAATAGGCCGCATGTGCCCCCTGCAATAAACAAACGCAGGCCAGAAAGCGCCAGTTCTGGCGAACCAgc                 <  1:81895/140‑1 (MQ=255)                                                                                                                               gACCACAAATCCCCCACCGCCGAGGCCGAGTAGCCAGCTGCCTGCCAGTAAATGGCGCTAAAACCGTAATAGGCCGCATGTGCCCCCTGCAATAAACAAACGCAGGCCAGAAAGCGCCAGTTCTGGCGAACCAGCGCCaa            <  2:666617/140‑1 (MQ=255)                                                                                                                               gACCACAAATACCCCACCGCCGAGGCCGAGTAGCCAGCTGCCTGCCAGTAAATGGCGCTAAAACCGTAATAGGCCGCATGTGCCCCCTGCAATAAACAAACGCAGGCCAGAAAGCGCCAGTTCTGGCGAACCAGCGCCaa            >  1:169816/1‑140 (MQ=255)                                                                                                                                  cacaAATCCCCCACCGCCGAGGCCGAGTAGCCAGCTGCCTGCCAGTAAATGGCGCTAAAACCGTAATAGGCCGCATGTGCCCCCTGCAATAAACAAACGCAGGCCAGAAAGCGCCAGTTCTGGCGAACCAGCGCCAACCa         <  2:103702/140‑1 (MQ=255)                                                                                                                                  cacaAATACCCCACCGCCGAGGCCGAGTAGCCAGCTGCCTGCCAGTAAATGGCGCTAAAACCGTAATAGGCCGCATGTGCCCCCTGCAATAAACAAACGCAGGCCAGAAAGCGCCAGTTCTGGCGAACCAGCGCCAACCa         >  1:1116535/1‑140 (MQ=255)                                                                                                                                  cacaAATACCCCACCGCCGAGGCCGAGTAGCCAGCTGCCTGCCAGTAAATGGCGCTAAAACCGTAATAGGCCGCATGTGCCCCCTGCAATAAACAAACGCAGGCCAGAAAGCGCCAGTTCTGGCGAACCAGCGCCAACCa         >  1:1065482/1‑140 (MQ=255)                                                                                                                                  cacaAATACCCCACCGCCGAGGCCGAGTAGCCAGCTGCCTGCCAGTAAATGGCGCTAAAACCGTAATAGGCCGCATGTGCCCCCTGCAATAAACAAACGCAGGCCAGAAAGCGCCAGTTCTGGCGAACCAGCGCCAACCa         >  1:86783/1‑140 (MQ=255)                                                                                                                                         aCCCCACCGCCGAGGCCGAGTAGCCAGCTGCCTGCCAGTAAATGGCGCTAAAACCGTAATAGGCCGCATGTGCCCCCTGCAATAAACAAACGCAGGCCAGAAAGCGCCAGTTCTGGCGAACCAGCGCCAACCACGCAGAc  >  1:1370936/1‑140 (MQ=255)                                                                                                                                         |                                                                                                                                            GCTCCCATAATGCCCCAGCGCACTACGCCGCAAATCGCCGAGATCAACAGCATATCGCGTGCACTACAACGGCGGAAAAGTTTATTACTCAGCGCAAAGATAATGACTTCCGCCACCACGCCCAGCGACCACAAATACCCCACCGCCGAGGCCGAGTAGCCAGCTGCCTGCCAGTAAATGGCGCTAAAACCGTAATAGGCCGCATGTGCCCCCTGCAATAAACAAACGCAGGCCAGAAAGCGCCAGTTCTGGCGAACCAGCGCCAACCACGCAGAC  >  NC_000913/2666990‑2667265

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑