| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_000913 | 3,206,435 | 0 | C | T | 25.5% | 104.2 / 19.6 | 51 | intergenic (‑179/‑28) | ttdR/ttdA | DNA‑binding transcriptional activator Dan/L(+)‑tartrate dehydratase subunit alpha |
| Reads supporting (aligned to +/- strand): ref base C (2/36); new base T (13/0); total (15/36) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 2.20e-10 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00 | |||||||||||
| Rejected as consensus: Frequency below/above cutoff threshold. | |||||||||||
AAAACATTGCCATTCCCTGCTCTGGCGCAATATTCATTCTTATTTTCGCGTTGATTCACGAACTGTTAATTACATATTCACGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGGT‑G‑AATTTTTCCA‑GACAAATACAAAAACTGGAGTTGCCATGATGAGCGAAAGTAATAAGCAACAGGCAGTGAATAAGTTGACAGAGATTGTCGCTAACTTTACCGCCATGATTTCTACCCGAATGCCTGATGACGTGGTGGA > NC_000913/3206298‑3206566 | agacagttGCCATTCCCTGCTCTGGCGCAATATTCATTCTTATTTTCGCGTTGATTCACGAACTGTTAATTACATATTCACGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGGT‑G‑AATTTTTCCa < 1:1025450/134‑1 (MQ=255) cATTGCCATTCCCTGCTCTGGCGCAATATTCATTCTTATTTTCGCGTTGATTCACGAACTGTTAATTACATATTCACGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGGT‑G‑AATTTTTCCA‑GACa < 2:885525/140‑1 (MQ=255) aTTGCCATTCCCTGCTCTGGCGCAATATTCATTCTTATTTTCGCGTTGATTCACGAACTGTTAATTACATATTCACGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGGT‑G‑AATTTTTCCA‑GACaa < 1:61565/140‑1 (MQ=255) tGCCATTCCCTGCTCTGGCGCAATATTCATTCTTATTTTCGCGTTGATTCACGAACTGTTAATTACATATTCACGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGGT‑G‑GATTTTTTCA‑GACaaaa > 2:941537/1‑139 (MQ=255) ccATTCCCTGCTCTGGCGCAATATTCATTCTTATTTTCGCGTTGATTCACGAACTGTTAATTACATATTCACGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGGT‑G‑AATTTTTCCA‑GACAAATAc < 1:990182/140‑1 (MQ=255) ccATTCCCTGCTCTGGCGCAATATTCATTCTTATTTTCGCGTTGATTCACGAACTGTTAATTACATATTCACGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGGT‑G‑AATTTTTCCA‑GACAAATAc < 2:594967/140‑1 (MQ=255) tGCTCTGGCGCAATATTCATTCTTATTTTCGCGTTGATTCACGAACTGTTAATTACATATTCACGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGGG‑G‑GATTTTTTCA‑Caaaaaaaaaaaatttt > 2:1025450/1‑136 (MQ=255) gCTCTGGCGCAATATTCATTCTTATTTTCGCGTTGATTCACGAACTGTTAATTACATATTCACGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGGT‑G‑AATTTTTCCA‑GACAAATACAAAAACTgg < 1:1138414/140‑1 (MQ=255) cAATATTCATTCTTATTTTCGCGTTGATTCACGAACTGTTAATTACATATTCACGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGGT‑G‑AATTTTTTCA‑GACAAATACAAAAACTGGAGTTGCCat > 1:953431/1‑140 (MQ=255) attcattcTTATTTTCGCGTTGATTCACGAACTGTTAATTACATATTCACGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGGT‑G‑AATTTTTCCA‑GACAAATACAAAAACTGGAGTTGCCatgatg < 2:642698/140‑1 (MQ=255) ttcattcTTATTTTCGCGTTGATTCACGAACTGTTAATTACATATTCACGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGGT‑G‑AATTTTTCCA‑GACAAATACAAAAACTGGAGTTGCCAtgatga < 2:295727/140‑1 (MQ=255) cattcTTATTTTCGCGTTGATTCACGAACTGTTAATTACATATTCACGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGTGGT‑G‑AATTTTTCCA‑GACAAATACAAAAACTGGAGTTGCCATGATGAGc < 2:627364/140‑1 (MQ=255) cattcTTATTTTCGCGTTGATTCACGAACTGTTAATTACATATTCACGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGGT‑G‑GATTTTTTCC‑AAAAAAAAAAAAAAATGGAGGTTGCATTATGAgg > 2:704484/1‑139 (MQ=255) ttcTTATTTTCGCGTTGATTCACGAACTGTTAATTACATATTCACGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGGT‑G‑AATTTTTCCA‑GACAAATACAAAAACTGGAGTTGCCATGATGAGCGa < 1:562430/140‑1 (MQ=255) ttcTTATTTTCGCGTTGATTCACGAACTGTTAATTACATATTCACGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGGT‑G‑AATTTTTCCA‑GACAAATACAAAAACTGGAGTTGCCATGATGAGCGa < 1:24402/140‑1 (MQ=255) tATTTTCGCGTTGATTCACGAACTGTTAATTACATATTCACGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGGT‑G‑AATTTTTTCATAACAAAACAAAAAAATGAAGTTGCCA‑GATGAggggaaaa > 1:1387400/1‑133 (MQ=255) tATTTTCGCGTTGATTCACGAACTGTTAATTACATATTCACGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGGT‑G‑AATTTTTTCA‑GACAAAAAAAAAAAACTGAGTTGTCATGATGAGCGAAAga > 1:769463/1‑139 (MQ=255) ttttCGCGTTGATTCACGAACTGTTAATTACATATTCACGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGGT‑G‑AATTTTTTCA‑GACAAAAACAAAAAATGGAGTTGCCATGATGAGCGAAAGgaa > 1:582860/1‑140 (MQ=255) ttttCGCGTTGATTCACGAACTGTTAATTACATATTCACGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGGT‑G‑AATTTTTTCA‑GACAAAAAAAAAAAATGGAGTTGCCATGATGAGCGGAAGaaa > 1:4523/1‑140 (MQ=255) ttttCGCGTTGATTCACGAACTGTTAATTACATATTCACGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGGT‑G‑AAATTTTTCA‑GACAAAAAAAAAAAATTGAGGTGTCATGATTAGGGGAAGtaa > 2:178486/1‑140 (MQ=255) tttCGCGTTGATTCACGAACTGTTAATTACATATTCACGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGGT‑G‑AATTTTTCCA‑GACAAATACAAAAACTGGAGTTGCCATGATGAGCGAAAGtaat < 2:536892/140‑1 (MQ=255) tttCGCGTTGATTCACGAACTGTTAATTACATATTCACGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGGT‑G‑AATTTTTCCA‑GACAAATACAAAAACTGGAGTTGCCATGATGAGCGAAAGtaat < 2:1127499/140‑1 (MQ=255) cgcgTTGATTCACGAACTGTTAATTACATATTCACGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGGT‑G‑AAATTTTTCA‑GACAAATACAAAAAATGGAGTTGCCATGATGAGCGAAAAgaataaa > 1:1082721/1‑139 (MQ=255) aTTCACGAACTGTTAATTACATATTCACGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGGT‑G‑AATTTTTCCA‑GACAAATACAAAAACTGGAGTTGCCATGATGAGCGAAAGTAATAAGCAACAgg < 2:912862/140‑1 (MQ=255) aTTCACGAACTGTTAATTACATATTCACGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGGT‑G‑AATTTTTCCA‑GACAAATACAAAAACTGGAGTTGCCATGATGAGCGAAAGTAATAAGCAACAgg < 1:704484/140‑1 (MQ=255) ttCACGAACTGTTAATTACATATTCACGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGGT‑G‑AACTTTTCCA‑GACAAATACAAAAACTGGAGTTGCCATGATGAGCGAAAGTAATAAGCAACAGGc < 2:809380/140‑1 (MQ=255) tCACGAACTGTTAATTACATATTCACGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGGT‑G‑AATTTTTCCA‑GACAAATACAAAAACTGGAGTTGCCATGATGAGCGAAAGTAATAAGCAACAGGCa < 2:953431/140‑1 (MQ=255) aaCTGTTAATTACATATTCACGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGGT‑G‑AATTTTTCCA‑GACAAATACAAAAACTGGAGTTGCCATGATGAGCGAAAGTAATAAGCAACAGGCAGTGaa < 1:941537/140‑1 (MQ=255) aaCTGTTAATTACATATTCACGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGGT‑G‑AATTTTTCCA‑GACAAATACAAAAACTGGAGTTGCCATGATGAGCGAAAGTAATAAGCAACAGGCAGTGaa < 2:918076/140‑1 (MQ=255) cTGTTAATTACATATTCACGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGGT‑G‑AATTTTTCCA‑GACAAATACAAAAACTGGAGTTGCCATGATGAGCGAAAGTAATAAGCAACAGGCAGTGAATa < 1:843111/140‑1 (MQ=255) cTGTTAATTACATATTCACGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGGT‑G‑AATTTTTCCA‑GACAAATACAAAAACTGGAGTTGCCATGATGAGCGAAAGTAATAAGCAACAGGCAGTGAATa < 2:1381773/140‑1 (MQ=255) tGTTAATTACATATTCACGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGGT‑G‑AATTTTTCCA‑GACAAATACAAAAACTGGAGTTGCCATGATGAGCGAAAGTAATAAGCAACAGGCAGTGAATaa < 1:1338686/140‑1 (MQ=255) gTTAATTACATATTCACGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGGT‑G‑AATTTTTCCA‑GACAAATACAAAAACTGGAGTTGCCATGATGAGCGAAAGTAATAAGCAACAGGCAGTGAATAAg < 2:1048556/140‑1 (MQ=255) gTTAATTACATATTCACGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGGT‑G‑AATTTTTCCA‑GACAAATACAAAAACTGGAGTTGCCATGATGAGCGAAAGTAATAAGCAACAGGCAGTGAATAAg < 1:716621/140‑1 (MQ=255) aaTTACATATTCACGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGGT‑G‑AATTTTTCCA‑GACAAATACAAAAACTGGAGTTGCCATGATGAGCGAAAGTAATAAGCAACAGGCAGTGAATAAGTTg < 1:292344/140‑1 (MQ=255) atatTCACGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGGT‑G‑AATTTTTTCA‑GACAAAAAAAAAAAATGGAGGTGCCATGATGAGCGAAAGTAAAAAACAAAAAGCAGGGAATAAATTGAAagag > 2:764185/1‑140 (MQ=255) atTCACGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGGTGG‑AATTTTTCC‑‑TACAAAAAAAAAAAAAGGAGGTGTCAGGATGAGGGAAAATAAAAAAAAAAAAGGAGAGAAAAAGTTGACAGAGAt > 2:292344/1‑140 (MQ=255) atTCACGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGGT‑G‑AATTTTTTCA‑GACAAAAAAAAAAAATGGAGGTGGCATGATGAGCGAAAAGAAAAAAAAACAAGCAGTGAATAAATTGACAgagaa > 2:970775/1‑139 (MQ=255) atTCACGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGGT‑G‑AATTTTTTCA‑GACAAAAAAAAAAAATGGAGGTGCCCTGATGAGCGAAAAGAAAAAACAAAAAGGAGAGAATAAAATTACAgagaa > 2:716621/1‑139 (MQ=255) atTCACGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGGT‑G‑AATTTTTTCA‑GACAAAAAAAAAAAAATGAGTTGGCATGATGAGCGGAAATAAAAAAAAAAAAGCAATGAATAAATTGACAgagaa > 2:82475/1‑139 (MQ=255) atTCACGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGGT‑G‑AATTTTTTCA‑GACAAAAAAAAAAAAAGGAGGTGCCCTGATGAGGGAAAGAAAAAAAAAAAAAGCAGAGAAAAAGTTGACAgagaa > 2:369738/1‑139 (MQ=255) atTCACGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGGT‑G‑AATTTTTTCA‑AACAAAAAAAAAAAATAGAGTTTGCATGATGAGCGGAAAAAATAAAAAACAAGCAGAGAATAAATTGACAgagaa > 2:951642/1‑139 (MQ=255) atTCACGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGGG‑G‑AATTTTTTTA‑GACAAATACAAAAAAAGGAGGTGCCCTGATGGGCGAAAAAAAAAAAAAACAAGCAGTGAAAAAATTGAAAgagaa > 2:661512/1‑139 (MQ=255) aCGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGGT‑G‑AATTTTTCCA‑GACAAATACAAAAACTGGAGTTGCCATGATGAGCGAAAGTAATAAGCAACAGGCAGTGAATAAGTTGACAGAGATTGTc < 1:694144/140‑1 (MQ=255) cGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGGT‑G‑AATTTTTCCA‑GACAAATACAAAAACTGGAGTTGCCATGATGAGCGAAAGTAATAAGCAACAGGCAGTGAATAAGTTGACAGAGATTGTCg < 2:644859/140‑1 (MQ=255) gCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGGT‑GAAATTTTTCCA‑‑ACAAAAAAAAAAAATGGAGGTGGCCTTATGAGCGAAAAAAAAAAACAACAGACAGGGAAAAAATTTACAAAGATTTTCGCTaaa > 2:504633/1‑139 (MQ=255) aaTATAAAACCACATTTTTAGTGGTAGTTTGTGGCGGT‑G‑AATTTTTCCA‑GACAAATACAAAAACTGGAGTTGCCATGATGAGCGAAAGTAATAAGCAACAGGCAGTGAATAAGTTGACAGAGATTGTCGCTAACTTTAcc < 1:1076939/140‑1 (MQ=255) aaTATAAAACCACATTTTTAGTGGTAGTTTGTGGCGGT‑G‑AATTTTTCCA‑GACAAATACAAAAACTGGAGTTGCCATGATGAGCGAAAGTAATAAGCAACAGGCAGTGAATAAGTTGACAGAGATTGTCGCTAACTTTAcc < 1:866040/140‑1 (MQ=255) ataAACCCACATTTTTAGTGGTAGTTTGTGGGGGT‑G‑ATTTTTTCCA‑GACAAATACAAAAACGGGAGTTGCCAGGATGAGCGAAAGTAAAAAGCAACAGGCAGTGAATAAGTTGACAGAGATTGTCGCTAACTTTACCGcc < 2:1387400/140‑1 (MQ=255) taAAACCACATTTTTAGTGGTAGTTTGTGGCGGT‑G‑AATTTTTTCA‑GACAAATACAAAAAATGGAGTTGCCATTATGAGAGAAAGTAATAAACAACAGGCAGTGAATAAATTGACAGAGATTTTCGCTAAATTTTCCGCCa > 2:1097409/1‑140 (MQ=255) taAAACCACATTTTTAGTGGTAGTTTGTGGCGGT‑G‑AATTTTTCCA‑GACAAATACAAAAACTGGAGTTGCCATGATGAGCGAAAGTAATAAGCAACAGGCAGTGAATAAGTTGACAGAGATTGTCGCTAACTTTACCGCCa < 1:10142/140‑1 (MQ=255) aaaCCACATTTTTAGTGGTAGTTTGTGGCGGT‑G‑ATTTTTTCCA‑GAAAAATACAAAAACTGGAGTTGCCATGATGAGCGAAAGTAATAAGCAACAGGCAGTGAATAAGTTGACAGAGATTGTCGCTAACTTTACCGCCATg < 2:769463/140‑1 (MQ=255) caTTTTTAGTGGTAGTTTGTGGCGGT‑G‑AATTTTTTCA‑GACAAAAACAAAAAATGGAGGTGTCATGATGAGCGGAAGGAAAAAAAAAAAGGCAGTGAATAAGATGACAGAGATTGTCGCTAAATTTAACGCCATGAttttt > 2:788993/1‑138 (MQ=255) caTTTTTAGTGGTAGTTTGTGGCGGT‑G‑AATTTTTTCA‑GACAAAAAAAAAAAATGGGGGTTCCCTGATGAGGGGAAATAAAAAAAAAAAAGCAGAGAAAAAAATGACAGAGATTTTCGCTAACTTTTCCCCCCTGAttttt > 2:1284572/1‑138 (MQ=255) caTTTTTAGTGGTAGTTTGTGGCGGA‑G‑TATTTTTTTA‑CACAAAAACAAAAAATGGAGGTGCCATGATGAGGGGAAAGAATAAACAACAAGCGGGGAAAAAGATGACAGAGATTGTTGCTCAACTTATCGCCAACAttttt > 2:842671/1‑138 (MQ=255) tAGTGGTAGTTTGTGGCGGT‑G‑AATTTTTCCA‑GACAAATAAAAAAACTGGAGTTGCCATGATGAGCGAAAGTAATAAGCAACAGGCAGTGAATAAGTTGACAGAGATTGTCGCTAACTTTACCGCCATGATTTCTACCCGa < 2:582860/140‑1 (MQ=255) gTGGTAGTTTGTGGCGGT‑G‑AATTTTTCCA‑GACAAATAAAAAAACTGGAGTTGCCATGATGAGCGAAAGTAATAAGCAACAGGCAGTGAATAAGTTGACAGAGATTGTCGCTAACTTTACCGCCATGATTTCTACCCGAAt < 1:970775/140‑1 (MQ=255) gTAGTTTGTGGCGGT‑G‑AATTTTTCCA‑GACAAATACAAAAACTGGAGTTGCCATGATGAGCGAAAGTAATAAGCAACAGGCAGTGAATAAGTTGACAGAGATTGTCGCTAACTTTACCGCCATGATTTCTACCCGAATGcc < 1:764185/140‑1 (MQ=255) gT‑G‑AATTTTTCCA‑GACAAATACAAAAACTGGAGTTGCCATGATGAGCGAAAGTAATAAGCAACAGGCAGTGAATAAGTTGACAGAGATTGTCGCTAACTTTACCGCCATGATTTCTACCCGAATGCCTGATGACgtggtg > 2:1087417/1‑140 (MQ=255) g‑AATTTTTCCA‑GACAAATACAAAAACTGGAGTTGCCATGATGAGCGAAAGTAATAAGCAACAGGCAGTGAATAAGTTGACAGAGATTGTCGCTAACTTTACCGCCATGATTTCTACCCGAATGCCTGATGACGTGGTGGa < 1:951642/140‑1 (MQ=255) g‑AATTTTTCCA‑GAAAAATACAAAAACTGGAGTTGCCATGATGAGCGAAAGTAATAAGCAACAGGCAGTGAATAAGTTGACAGAGATTGTCGCTAACTTTACCGCCATGATTTCTACCCGAATGCCTGATGACGTGGTGGa < 1:178486/140‑1 (MQ=255) | AAAACATTGCCATTCCCTGCTCTGGCGCAATATTCATTCTTATTTTCGCGTTGATTCACGAACTGTTAATTACATATTCACGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGGT‑G‑AATTTTTCCA‑GACAAATACAAAAACTGGAGTTGCCATGATGAGCGAAAGTAATAAGCAACAGGCAGTGAATAAGTTGACAGAGATTGTCGCTAACTTTACCGCCATGATTTCTACCCGAATGCCTGATGACGTGGTGGA > NC_000913/3206298‑3206566 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |